Life Sciences Commons^™

Characterization Of Transgenic Plum Lines Expressing Gastrodia Antifungal Protein (Gafp)-1, Hetalben Kalariya

All Theses

The Gastrodia antifungal protein (GAFP-1) is a mannose-binding lectin originating from the Asiatic orchid Gastrodia elata. It has potential for conferring resistance to fungal and non-fungal pathogens in other plants which is currently being investigated. The goals of this research project were to determine (i) the potential movement of GAFP-1 protein from transgenic rootstocks into the non-transgenic scion of chimeric-grafted trees (ii) the levels of GAFP-1 protein in lines of the cultivar Bluebyrd expressing the gene gafp-1 under the control of the polyubiquitin promoter bul409, and (iii) the susceptibility of selected lines to the root pathogens Phytophthora cinnamomi Rands and …

Sugar-Enhanced Sweet Corn Cultivar Evaluation For Northern Indiana, 2010, Elizabeth T. Maynard

Midwest Vegetable Trial Reports

Indiana growers harvested sweet corn for fresh market sales from 6,100 acres in 2009, with an average yield of 69 cwt/acre (164 crates or 3.45 tons per acre) and total value of $16.8 million (USDA NASS, 2010). Indiana ranks 14th among states for production of fresh market sweet corn. The 2007 USDA Ag Census reported 603 Indiana farms producing sweet corn for fresh markets and 51 farms selling to processors. Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bi-color corn is most commonly grown. Varieties with improved eating quality are of interest to …

Solutions To Soil Problems: Ii. High Ph (Alkaline Soil), Loralie Cox, Rich Koenig

All Current Publications

PH is a measure of the acidity or alkalinity of a material. The pH scale ranges from 0 to 14, with 7 being neutral. A pH value below 7 indicates the soil is acidic, while values above 7 are alkaline. Each unit change in the pH scale is a 10-fold difference in acidity or alkalinity. For example, soil with a pH of 8 is ten times more alkaline than soil with a pH of 7.

Solutions To Soil Problems: Iv. Soil Structure, Rich Koenig, Teresa Cerny

All Current Publications

Soil structure refers to the combination of primary soil particles – sand, silt and clay – into larger units called aggregates or clods. Aggregates are commonly seen when a soil is tilled or disturbed.

4-H Fun With Foods - Lesson 4: Beverages, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Naomi Weeks, Debra Proctor, Ronda Olsen

All Current Publications

This publication is part of the 4-H Fun with Foods Guide, Lesson 4 provides tips and recipes for making beverages.

4-H Fun With Foods - Lesson 6: Cookies: Molded, Refrigerator & Rolled, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Naomi Weeks, Debra Proctor, Ronda Olsen

All Current Publications

This publication is part of the 4-H Fun with Foods Guide, Lesson 6 provides recipes and instructions for molded, refrigerator, and rolled cookies.

4-H Fun With Foods - Lesson 7: Sandwiches, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Ronda Olsen, Naomi Weeks, Debra Proctor

All Current Publications

This publication is part of the 4-H Fun with Foods Guide, Lesson 7 provides recipes and instructions for making a variety of sandwiches.

Dutch Oven Cooking, Susan Haws

All Current Publications

This is a how-to publication on dutch oven cooking details.

Using Mulches In Utah Landscapes And Gardens, Rich Koenig, Kitt Farrell-Poe Phd, Bruce Miller

All Current Publications

This publication discusses the use of mulches in Utah landscapes including benefits and application information.

An Evaluation Of The Risk And Return Associated With Four Cattle Feeding Alternatives In Utah, Dillon M. Feuz, Caleb Bott

All Current Publications

This publication serves as an evaluation of the risk and return associated with four cattle feeding alternatives.

Equine Vision And Its Effect On Behavior, Patricia Evans

All Current Publications

This publication describes horses vision and how to work horses to the best advantage.

Managing Sodic Soils In Utah, D.W. James

All Current Publications

This publication discusses how to manage soil with excess sodium while it's becoming more prevalent in Utah.

Diagnostic Testing For Nitrogen Soil Fertility, D.W. James

All Current Publications

This publication defines the different types of diagnostic testing for nitrogen soil fertility.

Urea: Low Cost Fertilizer, D.W. James

All Current Publications

This fact sheet briefly describes urea transformations and suggests how urea-N may be conserved with proper management in the field.

Calibrating And Operating Manure Spreaders, Process Improvement For Animal Feeding Operations, John Harrison, Rich Koenig, Kerry Goodrich

All Current Publications

This guide outlines simplified methods to calibrate manure spreaders based on determining the load and area covered by the load.

Backyard Composting In Utah, Kitt Farrell-Poe, Rich Koenig

All Current Publications

This publication explains the benefits of composting and how to successfully start a compost in your own backyard.

Protein Preservation In Fossil Whale Bones Of The Miocene/Pliocene Pisco Formation, Peru, Uriel Leme Vidal

Loma Linda University Electronic Theses, Dissertations & Projects

Fossil remains have recently been the focus of considerable attention due to several independent reports of protein and soft-tissue preservation in ancient biological remains. The fossil whales from the Pisco Formation in Peru were previously reported to be very well preserved. A considerable percentage of these whales have their bones still articulated, and not a few have fossil baleen in life position. In this study we assayed bone samples from some fossil whales for ultrastructure preservation and the presence of original protein/proteinaceous materials. For analysis of fine details of structural preservation we used light microscopy and Scanning Electron Microscopy (SEM). …

2010 Final Oregon Harvest Report, Oregon Wine Board

Oregon Wine Board Documents

This harvest report for the Oregon wine industry from 2010 describes a vintage with high-quality fruit and lower yields. Warm and dry conditions late in the growing season allowed advanced ripening resulting in full flavor development, lower brix levels, great acidity, and potentially lower alcohol levels.

Cell Lines Having Enhanced Cell Longevity And Protein Expression, Bruce Allen Webb, Jeremy Kroemer

Entomology Faculty Patents

It is the object of the current invention to provide methods and compositions relating to the expression of vankyrin proteins in cell lines to increase their viability, longevity and capacity for protein production. The inventors have discovered that the expression of P-ank-1 and I²-ank-3 proteins in cell culture has increased the cells' longevity and capacity for endogenous and/or heterologous target protein production. Specifically, the present invention relates to the enhanced expression of endogenous and/or heterologous target proteins/polypeptides in recombinant cells that are also expressing P-ank-1 and/or I²-ank-3 protein compared to expression host cells that are not …

Ocean Mass From Grace And Glacial Isostatic Adjustment, D. Chambers, John Wahr, Mark E. Tamisea, R Steven Nerem

Marine Science Faculty Publications

We examine geoid rates and ocean mass corrections from two published global glacial isostatic adjustment (GIA) models, both of which have been used in previous studies to estimate ocean mass trends from Gravity Recovery and Climate Experiment (GRACE) satellite gravity data. These two models are different implementations of the same ice loading history and use similar mantle viscosity profiles. The model results are compared with each other and with geoid rates determined from GRACE during August 2002 to November 2009. When averaged over the global ocean, the two models have rates that differ by nearly 1 mm yr⁻¹ of …

Effects Of Dietary Choline, Folic Acid And Vitamin B12 On Laying Hen Performance, Egg Components And Egg Phospholipid Composition, Pradeep Krishnan Rajalekshmy

Department of Animal Science: Dissertations, Theses, and Student Research

In trial 1, a corn-soy basal diet was formulated with three levels of supplemental choline (0, 500 and 1000 ppm) and three levels of supplemental folic acid (0, 2 and 4 ppm) in a 3 x 3 factorial arrangement. Folic acid at 2 ppm increased egg production (p ≤ 0.04). Egg wt was higher at 500 ppm of choline (p ≤ 0.06) and 0 ppm (p ≤ 0.01) of folic acid supplementation. There were choline by folic acid interaction effects on feed intake (p ≤ 0.001), albumen wt (p ≤ 0.005) and yolk wt ( …

Excision Dynamics Of Vibrio Pathogenicity Island-2 From Vibrio Cholerae: Role Of A Recombination Directionality Factor Vefa, Salvador Almagro-Moreno, Michael G. Napolitano, E. Fidelma Boyd

Dartmouth Scholarship

Vibrio Pathogenicity Island-2 (VPI-2) is a 57 kb region present in choleragenic V. cholerae isolates that is required for growth on sialic acid as a sole carbon source. V. cholerae non-O1/O139 pathogenic strains also contain VPI-2, which in addition to sialic acid catabolism genes also encodes a type 3 secretion system in these strains. VPI-2 integrates into chromosome 1 at a tRNA-serine site and encodes an integrase intV2 (VC1758) that belongs to the tyrosine recombinase family. ntV2 is required for VPI-2 excision from chromosome 1, which occurs at very low levels, and formation of a non-replicative circular intermediate.

Requirement For Golgi-Localized Pi(4)P In Fusion Of Copii Vesicles With Golgi Compartments, Andres Lorente-Rodriguez, Charles Barlowe

Dartmouth Scholarship

The role of specific membrane lipids in transport between endoplasmic reticulum (ER) and Golgi compartments is poorly understood. Using cell-free assays that measure stages in ER-to-Golgi transport, we screened a variety of enzyme inhibitors, lipid-modifying enzymes, and lipid ligands to investigate requirements in yeast. The pleckstrin homology (PH) domain of human Fapp1, which binds phosphatidylinositol-4-phosphate (PI(4)P) specifically, was a strong and specific inhibitor of anterograde transport. Analysis of wild type and mutant PH domain proteins in addition to recombinant versions of the Sac1p phosphoinositide-phosphatase indicated that PI(4)P was required on Golgi membranes for fusion with coat protein complex II (COPII) …

Conditions Associated With Clostridium Sporogenes Growth As A Surrogate For Clostridium Botulinum In Non-Thermally Processed Canned Butter, Reed H. Taylor

Theses and Dissertations

Shelf-stable canned butter is currently available in retail stores, and many home-preservationists promote home-canning of butter. Non-cultured butter is a low-acid canned food, which would presumably require thermal processing. The lack of a thermal process step in canned butter products raises questions of potential safety, because they are hermetically sealed and generally exhibit anaerobic growth conditions, which are optimal for Clostridium botulinum growth. Without thermal processing, low-acid canned foods (LACF) must have inhibitory factors present to prevent C. botulinum growth. Some potential intrinsic inhibitory factors, or "hurdles", within butter include: reduced water activity (aw), acidity (pH) in cultured products, elevated …

A Bayesian Shared Component Model For Genetic Association Studies, Juan J. Abellan, Carlos Abellan, Juan R. Gonzalez

COBRA Preprint Series

We present a novel approach to address genome association studies between single nucleotide polymorphisms (SNPs) and disease. We propose a Bayesian shared component model to tease out the genotype information that is common to cases and controls from the one that is specific to cases only. This allows to detect the SNPs that show the strongest association with the disease. The model can be applied to case-control studies with more than one disease. In fact, we illustrate the use of this model with a dataset of 23,418 SNPs from a case-control study by The Welcome Trust Case Control Consortium (2007) …

Substrates Of The Arabidopsis Thaliana Protein Isoaspartyl Methyltransferase 1 Identified Using Phage Display And Biopanning, Tingsu Chen, Nihar Nayak, Susmita Maitra Majee, Jonathan Lowenson, Kim R. Schäfermeyer, Alyssa C. Eliopoulos, Taylor D. Lloyd, Randy Dinkins, Sharyn E. Perry, Nancy R. Forsthoefel, Steven G. Clarke, Daniel M. Vernon, Zhaohui Sunny Zhou, Tomas Rejtar, A. Bruce Downie

Horticulture Faculty Publications

The role of protein isoaspartyl methyltransferase (PIMT) in repairing a wide assortment of damaged proteins in a host of organisms has been inferred from the affinity of the enzyme for isoaspartyl residues in a plethora of amino acid contexts. The identification of PIMT target proteins in plant seeds, where the enzyme is highly active and proteome long-lived, has been hindered by large amounts of isoaspartate-containing storage proteins. Mature seed phage display libraries circumvented this problem. Inclusion of the PIMT co-substrate, S-adenosylmethionine (AdoMet), during panning permitted PIMT to retain aged phage in greater numbers than controls lacking co-substrate or when …

Minimum Description Length And Empirical Bayes Methods Of Identifying Snps Associated With Disease, Ye Yang, David R. Bickel

COBRA Preprint Series

The goal of determining which of hundreds of thousands of SNPs are associated with disease poses one of the most challenging multiple testing problems. Using the empirical Bayes approach, the local false discovery rate (LFDR) estimated using popular semiparametric models has enjoyed success in simultaneous inference. However, the estimated LFDR can be biased because the semiparametric approach tends to overestimate the proportion of the non-associated single nucleotide polymorphisms (SNPs). One of the negative consequences is that, like conventional p-values, such LFDR estimates cannot quantify the amount of information in the data that favors the null hypothesis of no disease-association.

We …

Guide To Woody Plants Of Nebraska, Justin R. Evertson, Kim Todd, David Mooter, Kate Schumacher

Nebraska Forest Service: Publications

Species diversity is the cornerstone of a healthy community forest. In an effort to increase species diversity, this publication provides a nearly complete list of woody plants that can be grown in Nebraska communities. Each plant is keyed to the hardiness zone map on this page to show where it will grow best in Nebraska. It should be noted, however, that many plants need extra care and protection to survive certain climates. Conversely, many plants may do well outside of their recommended zone if the proper microclimate exists.

How To Manage Your Woodlands For Sustained And Maximum Benefits, Steven D. Rasmussen

Nebraska Forest Service: Publications

Nebraska's forest lands comprise less than 2 percent of the total land base in the state (7 18,300 acres). However, on an acre by acre comparison, woodlands provide more associated benefits for society, the environment and our quality of life than most other land uses. Trees provide soil protection from wind and water erosion. Woodlands help protect the quality of adjacent water resources by eliminating contaminates, shading for cooler water temperatures and contributing organic matter for use by aquatic life at all levels. Trees and shrubs also trap snow for spring moisture. Woodlands are necessary areas for many wildlife species …

Nebraska Forest Service: Storm Damage Series, Nebraska Forest Service Storm Damage Bulletins

Nebraska Forest Service: Publications

Immediate Care for Storm Damaged Trees; How to Select an Arborist or Tree Service; Pruning Storm Damaged Trees; Large Tree Pruning and Care; Don’t Top Trees; Recognizing and Correcting Tree Hazards; Tree Selection and Placement; Tree Planting; Care of Newly Planted Trees; Storm Damage Resources;