Life Sciences Commons^™

Generation Of High Efficiency Ssdna Hybridization Probes By Linear Polymerase Chain Reaction (Lpcr), Gregory W. Konat

Scanning Microscopy

The polymerase chain reaction (PCR) methodology can be employed to produce DNA hybridization probes. The major advantages of this paradigm over other techniques include superior specific activity of the probes, the versatility of sequence selection, the ability to produce short probes, and the simplicity of the procedure. We have further improved the efficiency of PCR probes by generating single stranded (ssDNA) probes that do not reanneal with themselves in solution, and hence, their availability for the interaction with the complementary sequences of the target is profoundly increased. Protocols for ³²P-dCTP labeled and digoxigenin-dUTP labeled probes have been elaborated to …

Lippia Nodiflora (L.) Michx., John E. Ebinger

Specimens by Name

No abstract provided.

Meeting Minutes, July 9th, 1996, National Smokejumper Association Executive Committee

National Smokejumper Association Meeting Minutes

Agenda: April, May, & June minutes are not typed, can not be approved.; Treasurers Report; Membership Report; Historians Report; Newsletter; NSA/NFSM/MMF Joint Venture; Life Membership; Next Reunion in 2000; Poster Copy Rights & Membership List Sales; Old Business & Other Business; Inter Net user fees ; Selection of the newsletter printer; Newsletter Advertisements may jeopardize non-profit bulk mailing rates; Help with membership; Video Report;

Calopogon Tuberosus (L.) Britton, Sterns & Poggenb., Vern Lagesse

Specimens by Name

No abstract provided.

Hexapod Herald - Vol. 8, No. 10, July 9, 1996

Hexapod Herald and Other Entomology Department Newsletters

Grants

Congratulations

Graduate student news

Departmental travelers

News from the office

Miscellaneous

Calendar of events

Feeding Habits Of Juvenile Largemouth Bass Micropterus Salmoides (Lacepede), Thomas R. Goodwill

Honors Theses

The stomachs of 110 juvenile largemouth bass, Micropterus salmoides (Lacepede) were examined to learn the early feeding habits of bass in a brackish environment. Bass were found to have five primary food sources: Taphromysis bowmani, Cloeon sp., Gammarus mucronatus, Micropterus salmoides, and Lepidophthalmus louisianum. Three feeding trends were observed during the course of the study. First, bass seemed to display prey selection. Second, bass length increased as prey size increased. Third, the types of prey eaten by the bass changed as the bass increased in size.

Expression Of A Bovine Κ-Cn Cdna In The Mammary Gland Of Transgenic Mice Utilizing A Genomic Milk Protein Gene As An Expression Cassette, Alfonso Gutiérrez, Harry M. Meade, Paul Ditullio, Daniel Pollock, Merry Harvey, Rafael Jiménez-Flores, Gary B. Anderson, James D. Murray, Juan F. Medrano

Dairy Science

Transgenic mice were produced by microinjection of a DNA construct composed of the bovine κ-casein (κ-CN) cDNA under the control of the goat β-CN 5' promoter elements and 3' flanking regions into pronuclear-stage embryos. The gene construct targeted the expression of bovine κ-CN RNA to the mammary gland and secretion of bovine κ-CN in the milk. In the three lines studied (BC-7, BC-31 and BC-67) the transgene was stably integrated and propagated as a Mendelian locus. Expression of the bovine protein in lactating mice from the three transgenic lines was demonstrated by northern and western blots. In ten different tissues …

Lupin Logic Number 72

Lupin Logic

Contents

Post-emergence broadleaf weed control in lupins Terry Piper

- Grass weeds

Lupin production in Germany Peter Roemer, Suedwestdeutsche Saatzucht

Forecasting aphid outbreaks and virus epidemics Dr Debbie Thackeray

Vital reading

South Dakota Farm And Home Research, South Dakota State University

South Dakota Farm and Home Research: 1949 -1998

Director's comments: We are still pioneers [p] 1
NIRS allows fast, accurate, non-destructive grain analysis [p] 2
Meat snacks are another wayto add value to beef [p] 4
Tailoring a grain marketing plan for highest revenue [p] 6
South Dakota banks in good shape and ready to serve ag [p] 8
Bringing South Dakota State University to you [p] 10
Study seeks best rotations and tillage methods for southeast South Dakota [p] 12
Rough soil surface can double forage yield [p] 14
'Skinny cow disease' poses on-going probleD1 for cattle Industry [p] 16
Risk of E. call 0157:H7 in South …

Santa Clara Magazine, Volume 38 Number 3, Summer 1996, Santa Clara University

Santa Clara Magazine

6 - THE BEATEN TRACK SCU Psychologist Stephanie Etukudo and Senior Lecturer in English Jeffrey Zorn debate whether ability groupings serve students' best interests.

12 - WHAT WOULD SOLOMON SAY? Adoptions gone awry trigger controversy over how to safeguard the rights of parents while protecting the welfare of the child. By Elizabeth Fernandez '79

18 - A THIRST FOR HEALING A pilgrim to Lourdes finds that the cures offered by the blessed waters can be mostly invisible. By Dee Danna '68 (MBA '72)

24 - THE FLOWERING OF SANTA CLARA UNIVERSITY For more than a century, the Mission Gardens have …

Nebline, July 1996

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Horticulture

Rural $ense

Family Living

4-H & Youth

Environmental Focus

Community & Leadership Development

Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Nebraska Association for Family and Community Education News

and other extension news and events

Habitat Sampling And Habitat Selection By Female Wild Turkeys: Ecological Correlates And Reproductive Consequences, A. V. Badyaev, Thomas E. Martin, W. J. Etges

Wildlife Biology Faculty Publications

Habitat sampling can allow much more effective habitat selection for longterm activities such as nesting and may be directly linked to fitness. We studied the process of habitat sampling and selection in female Wild Turkeys (Meleagris gallopavo) in the Arkansas Ozarks. In particular, we tested the prediction that movements prior to selecting nesting habitat correlate with the quality of selected habitat. Our results supported the prediction that greater habitat sampling (as reflected by greater area covered prior to nesting) allows acquisition of better nesting habitat; greater movements were correlated with choice of better nesting sites with more cover that allow …

Evidence For Acquisition In Nature Of A Chromosomal 2,4-Dichlorophenoxyacetic Acid/(Alpha)-Ketoglutarate Dioxygenase Gene By Different Burkholderia Spp., V. Grace Matheson, Larry J. Forney, Yuichi Suwa, Cindy H. Nakahatsu, Alan J. Sexstone, William E. Holben

Biological Sciences Faculty Publications

We characterized the gene required to initiate the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) by the soil bacterium Burkholderia sp. strain TFD6, which hybridized to the tfdA gene of the canonical 2,4-D catabolic plasmid pJP4 under low-stringency conditions. Cleavage of the ether bond of 2,4-D by cell extracts of TFD6 proceeded by an (alpha)-ketoglutarate-dependent reaction,characteristic of TfdA (F. Fukumori and R. P. Hausinger, J. Bacteriol. 175:2083-2086, 1993). The TFD6 tfdA gene was identified in a recombinant plasmid which complemented a tfdA transposon mutant of TFD6 created by chromosomal insertion of Tn5. The plasmid also expressed TfdA activity in Escherichia coli …

Characterization Of A Chromosomally Encoded 2,4-Dichlorophenoxyacetic Acid/A-Ketoglutarate Dioxygenase From Burkholderia Sp. Strain Rasc, Yuichi Suwa, Alice D. Wright, Fumitasu Fukimori, Kathy A. Nummy, Robert P. Hausinger, William E. Holben, Larry J. Forney

Biological Sciences Faculty Publications

The findings of previous studies indicate that the genes required for metabolism of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) are typically encoded on broad-host-range plasmids. However, characterization of plasmid-cured strains of Burkholderia sp. strain RASC, as well as mutants obtained by transposon mutagenesis, suggested that the 2,4-D catabolic genes were located on the chromosome of this strain. Mutants of Burkholderia strain RASC unable to degrade 2,4-D (2,4-D- strains) were obtained by insertional inactivation with Tn5. One such mutant (d1) was shown to have Tn5 inserted in tfdA_RASC, which encodes 2,4-D/alpha-ketoglutarate dioxygenase. This is the first reported example of a …

Fisheries Research Report No 105 - The Western Rock Lobster Fishery 1986/87 To 1990/91, C. F. Chubb, E. H. Barker, R. S. Brown

Fisheries Research Reports

This report on the western rock lobster (Panulirus cygnus, George 1962) fishery covers the five seasons 1986/87 to 1990/91 and is the latest in a series of reports on the State's largest and most valuable fishery. While more detailed data bases exist, these reports form a historical record of basic data for use by both industry and research workers, now and in the future. Catch and fishing effort in the seasons (1986/87 to 1990/91) varied between 8500 and 12300 tonnes and 11.3 and 12.8 million pot lifts respectively. This report provides detail on the distribution of catches and fishing effort; …

Flea News 52 (July 1996), Robert E. Lewis

Flea News

Editorial, Robert E. Lewis

Miscellanea

Announcement

Siphonaptera literature

New flea species

Center For Grassland Studies Newsletter, Summer 1996, Volume 2, No. 3

Center for Grassland Studies: Newsletters

Contents:

Matching the Cow with Forage Resources by Don Adams, Richard Clark, Terry Klopfenstein, and Jerry Volesky

Grassland Biodiversity, Large Grazers and Fire by Al Steuter, The Nature Conservancy

American Forage and Grassland Council Conference by Lowell Moser, Department of Agronomy, UNL

CGS Citizens Advisory Council Holds Third Meeting In Western Nebraska

Grazing Lands Conservation Initiative Exploratory Meeting

Ministerial Policy Guideline No.3: Determining A "Fit And Proper Person" For Rock Lobster Processing Authorizations, Department Of Fisheries Wa

Fisheries Administrative Guidelines

The Ministerial Policy Guidelines were drafted by a working group appointed by the Minister for Fisheries, the Hon Monty House MLA. The Minister has now determined these Guidelines and issued them to the Department to help decide applications and similar matters relating to the licensing of rock lobster processors in Western Australia.

1: "To Know Ourselves", The U.S. Department Of Energy, The Human Genome Project

Genomics: Past & Future

AT THE END OF THE ROAD in Little Cottonwood Canyon, near Salt Lake City, Alta is a place of near-mythic renown among skiers. In time it may well assume similar status among molecular geneticists. In December 1984, a conference there, co-sponsored by the U.S. Department of Energy, pondered a single question: Does modern DNA research offer a way of detecting tiny genetic mutations—and, in particular, of observing any increase in the mutation rate among the survivors of the Hiroshima and Nagasaki bombings and their descendants? In short the answer was, Not yet. But in an atmosphere of rare intellectual fertility, …

Oxidative Stress Detection With Escherichia Coli Harboring A Katg'::Lux Fusion, S. Belkin, D. R. Smulski, Amy Cheng Vollmer, T. K. Van Dyk, R. A. Larossa

Biology Faculty Works

A plasmid containing a transcriptional fusion of the Escherichia coli katG promoter to a truncated Vibrio fischeri lux operon (luxCDABE) was constructed. An E. coli strain bearing this plasmid (strain DPD2511) exhibited low basal levels of luminescence, which increased up to 1,000-fold in the presence of hydrogen peroxide, organic peroxides, redox-cycling agents (methyl viologen and menadione), a hydrogen peroxide-producing enzyme system (xanthine and xanthine oxidase), and cigarette smoke. An oxyR deletion abolished hydrogen peroxide-dependent induction, confirming that oxyR controlled katG'::lux luminescence. Light emission was also induced by ethanol by an unexplained mechanism. A marked synergistic response was observed when cells …

Female Choice In Sage Grouse: The Roles Of Attraction And Active Comparison, Robert M. Gibson

School of Biological Sciences: Faculty Publications

Previous studies of female choice in sage grouse Centrocercus urophasianus have implicated both the acoustic quality and repetition rate of the stereotyped strut display as putative cues for female choice. Stages in the choice process at which specific components of male courtship display influence female decisions were investigated using field observations of female premating behavior. Females visited a subset of territorial males and then actively chose one of these as a mate. The order in which males were visited suggested that females searched until an acceptable mate was found, rather than employing a “best-of-n” tactic. Numbers of females visiting a …

Case Study Of The Profitability Of A South Dakota Farm Using The Integrated Farm Managment Program, Charles L. Prouty, Thomas L. Dobbs

Economics Pamphlet Series (1985-2016)

This report is one of five covering case study farms in the east-central South Dakota portion of the Big Sioux Aquifer area. The other four reports are South Dakota State University Econ Pamphlets 95-1 through 95-4, published in September 1995. operators of each of the case study farms covered in the five reports were participating in some segment of the Federal farm program aimed specifically at improving the ecological sustainability of U.S. agriculture. The case farm featured in this report was participating in the Integrated Farm Management (IPM) program of the 1990 Farm Bill. Since the data collection and analyses …

Patch Diseases In Kentucky Bluegrass Lawns, Paul C. Vincelli, A. J. Powell Jr.

Agriculture and Natural Resources Publications

“Patch diseases” can be very destructive when Kentucky bluegrass is grown under intensive management. Two patch diseases with similar symptoms can occur. Necrotic ring spot often appears in early summer. Summer patch, the more common disease in Kentucky landscapes, develops in middle to late summer.

These diseases are often a concern in lawns under intensive management for two reasons:

1. Improper application of certain lawn management practices, such as close mowing, can aggravate disease development.
2. When a lawn receives significant inputs of fertilizer and irrigation and frequent mowing, the homeowner or landscape manager usually has high aesthetic expectations and tolerates …

Louisiana Agriculture Magazine, Summer 1996, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Impact Of A Parasitic Plant On The Structure And Dynamics Of Salt Marsh Vegetation, Steven C. Pennings, Ragan M. Callaway

Biological Sciences Faculty Publications

We investigated the effect of a native parasitic plant, Cuscuta salina, on the structure and dynamics of the plant community in a California salt marsh. Cuscuta was common in the middle marsh zones. The abundance of Cuscuta was positively correlated with the abundance of Limonium californicum at two sampling scales (0.25— and ≈\approx40—m² quadrats). Sampling at the scale of individual plants indicated that the dominant plant in the marsh, Salicornia virginica, was preferred by Cuscuta as a host over Arthrocnemum subterminale, Limonium californicum, and Frankenia salina. This result was confirmed with host—choice experiments in the field. Based on spatial …

The Real Cost Of Beef: A Monetary Assessment Of The Environmental Degradation Caused By Beef Production, Carolyn Babcock

UNLV Theses, Dissertations, Professional Papers, and Capstones

The environmental degradation caused by beef production is severe. The current literature assesses the ecological damage, but falls short of assigning a per pound dollar amount to reflect the real cost of beef. In the United States, most of the environmental focus has been centered around the use of public lands for grazing and the grazing fee the government considers appropriate. The fee covers the maintenance of the grazing program, which includes maintenance of the actual land. This paper will concentrate on beef-related environmental degradation and select specific damage for monetary valuation.

Morphometry Of Juvenile And Subadult Loligo Pealei And L. Plei From The Northern Gulf Of Mexico, Guillermo Sánchez, Harriet M. Perry, Christine B. Trigg, Michael Vecchione, Clyde Roper, Patricia M. Biesot

Faculty Publications

Two species of Loligo are abundant in northern Gulf waters: the long-finned squid, Loligo pealei. and the arrow squid, Loligo plei. Variability within species and similarities between the species often hamper accurate identification. The two species more closely resemble each other in areas of sympatry. and there is overlap in almost all of the diagnostic characters. Small specimens of Loligo are not easily identified. and there are few studies detailing their morphometry. Because of the taxonomic uncertainties associated with the identification of juveniles and subadults of L. pealei and L. plei, the species were differentiated by isoelectric …

Characterization Of A Newly Cloned Dna Damage- Inducible Gene From Saccharomyces Cerevisiae Strain D43, June-Home Chen

Master's Theses

The identification and characterization of DNA damage-inducible genes is an important topic in the study of the integrity of the cellular genome. Previously, a TnlO-lacZ derivative was inserted randomly into yeast cells and the resulting mutants were screened for insertions in DNA damage-inducible genes. S. cerevisiae strain D43 was identified as one such TnlO-lacZ insertion mutant. DNA sequencing results show the insertion in D43 is located within the TDH2 gene, which encodes glyceraldehyde-3-phosphate dehydrogenase. Treatment of strain D43 with DNA damaging agents and heat shock suggest TDH2 is responsive to DNA damage, but not to heat shock. Haploid mutants carrying …

Lead Activation Of A Developmentally Regulated Calcium Channel In Rat Hippocampal Nerve Terminals, Troy E. Rhodes

Theses and Dissertations in Biomedical Sciences

Low level lead (Pb²⁺) exposure may produce lasting deficits in learning and memory by altering calcium (Ca²⁺) dependent processes. Isolated presynaptic nerve terminals from rat hippocampus were loaded with the intracellular (Ca²⁺) indicator Fura-2. The changes in cytoplasmic free calcium ([Ca²⁺]_i) were measured by stopped-flow fluorescence spectroscopy following depolarization with elevated potassium on a millisecond time scale (Lentzner et al., 1992). Depolarization promoted a rapid increase in Ca²⁺_i which occured in two kinetically distinguishable phases: a fast component, representing the activity of rapidly inactivating Ca²⁺ channels (τ …

Linking Local Species Interactions To Rates Of Speciation In Communities, Mark A. Mcpeek

Dartmouth Scholarship

Regional species diversity limits the diversity of local communities by defining the pool of species that are available to colonize sites. Biogeographical processes that influence speciation and extinction rates determine the size and composition of this regional species pool. Community ecologists are beginning to recognize the importance of these biogeographical processes in influencing diversity patterns among local communities, but the potential for local interactions to influence biogeographical processes, especially speciation, has been little considered. In this paper I discuss one mechanism by which variation in the strengths of local interactions can influence the potential for population differentiation and thus for …