Life Sciences Commons^™

Parthenocissus Tricuspidata (Siebold & Zucc.) Planch., Gordon C. Tucker

Specimens by Name

No abstract provided.

Advanced Instrumentation And Methodology Related To Cryoultramicrotomy: A Review, H. Sitte

Scanning Microscopy

This review is concerned with the considerable progress in the field of cryo-ultramicrotomy (cryofixation, cryosectioning, investigation and analysis of cryosections) during recent years. This progress includes both more efficient instrumentation and methodology. The article is mainly directed to the investigation and analysis of frozen-hydrated sections in the low dose cryo-transmission electron microscopy (TEM) and cryo-energy filtered TEM (EFTEM). A general survey is followed by an evaluation of the different relevant procedures. Both cryo-ultramicrotomy for macromolecular cytochemistry (Tokuyasu technique) and cryo-ultramicrotomy for element analysis are only shortly mentioned without discussion of the chemical and analytical approach. Because of lack of first …

Cytoskeletal Changes During Radiation-Induced Neoplastic Transformation Of Human Prostate Epithelial Cells, S. C. Prasad, P. J. Thraves, J. S. Rhim, M. R. Kuettel

Scanning Microscopy

We recently reported tumorigenic transformation of SV 40-immortalized neonatal human prostate epithelial cells (267Bl) by exposure to fractionated doses of X-rays. Altered morphology and anchorage independence were observed following two successive fractions of 2 Gy each (F3-SAC). Additional 2 Gy treatments to these non-tumorigenic cells to a total dose of 30 Gy resulted in radiation-transformed tumorigenic colonies (267Bl-SXR). Malignant transformation of parental 267B 1 cells was also achieved by consecutive 2 Gy exposures to a total dose of 30 Gy (267Bl-XR). This study discusses the cytoskeletal changes in the F3-SAC, 267Bl-XR and 267Bl-SXR derivatives of these human prostate epithelial cells. …

Evaluation Of A Rapid-Screening Toxicity Test Using The Ciliate, Colpoda Inflata (Stokes): Sensitivity And Bioavailability To Model Compounds, Daria Galina Mochan

Dissertations and Theses

Standard toxicity tests often require high costs for maintaining healthy cultures, so few test species are employed in routine ecotoxicological analysis. An alternative is the "battery of tests" approach involving using rapid toxicity tests for screening. Rapid-screening toxicity tests must display organism sensitivity, similarity in responses to other test organisms, relevancy to many circumstances, and repeatability. Protozoa are ideal candidates for rapid-screening bioassays. They are cosmopolitan, play important roles in ecosystems, and have high reproductive rates. Many protozoa can form a resting stage (cyst) that remains viable during adverse conditions, eliminating the need for maintaining continuous cultures for testing.

The …

Hexapod Herald - Vol. 8, No. 11, August 7, 1996

Hexapod Herald and Other Entomology Department Newsletters

Grants

Graduate student news

Welcome

Farewell

Congratulations

From Cheri's desk

Calendar of events

Thermal Preference Of Spring And Fall Chinook Salmon (Oncorhynchus Tshawytscha) During Smoltification, Sally T. Sauter

Dissertations and Theses

Innate species-specific temperature preferences of fish are subjected to fluctuations under a variety of environmental, physiological, and developmental conditions. The temperature preference patterns of two ecologically distinct races of chinook salmon (Oncorhynchus tshawytscha) were investigated as laboratory held animals underwent smoltification. Smoltification is a distinct developmental stage in the life history of anadromous salmonids when juvenile fish undergo profound behavioral, morphological, and physiological differentiation prepatory to seawater entry. A group of spring and fall chinook salmon were held under identical conditions of increasing water temperature over the course of smoltification. Another group of spring and fall chinook salmon were held …

Cropwatch No. 96-18, Aug. 2, 1996, Lisa Brown Jasa

Crop Watch

Inside

Evapotranspiration data ...............126

Pest update...............126

Use care near bees ............... 127

European corn borers...............128

Armyworms in Perkins County...............129

Cercospora in sugar beets...............130

Mutations In Two Ku Homologs Define A Dna End-Joining Repair Pathway In Saccharomyces Cerevisiae, George Todd Milne, Shengfang Jin, Katie Shannon, David T. Weaver

Biological Sciences Faculty Research & Creative Works

DNA double-strand break (DSB) repair in mammalian cells is dependent on the Ku DNA binding protein complex. However, the mechanism of Ku-mediated repair is not understood. We discovered a Saccharomyces cerevisiae gene (KU80) that is structurally similar to the 80-kDa mammalian Ku subunit. Ku80 associates with the product of the HDF1 gene, forming the major DNA end-binding complex of yeast cells. DNA end binding was absent in ku80Δ, hdf1Δ, or ku80Δ hdf1Δ strains. Antisera specific for epitope tags on Ku80 and Hdf1 were used in supershift and immunodepletion experiments to show that both proteins …

The Bacillus Thuringiensis Insecticidal Toxin Binds Biotin-Containing Proteins, Cheng Du, Kenneth Nickerson

Papers in Microbiology

Brush border membrane vesicles from larvae of the tobacco hornworm, Manduca sexta, contain protein bands of 85 and 120 kDa which react directly with streptavidin conjugated to alkaline phosphatase. The binding could be prevented either by including 10 μM biotin in the reaction mixture or by prior incubation of the brush border membrane vesicles with an activated 60- to 65-kDa toxin from Bacillus thuringiensis HD-73. The ability of B. thuringiensis toxins to recognize biotin-containing proteins was confirmed by their binding to pyruvate carboxylase, a biotin-containing enzyme, as well as to biotinylated ovalbumin and biotinylated bovine serum albumin but not …

Interleukin-10 Inhibits Tumor Metastasis Through An Nk-Cell Dependent Mechanism, Li-Mou Zheng, David M. Ojcius, F. Garaud, C. Roth, E. Maxwell, Z. Li, H. Rong, J. Chen, X. Y. Wang, J. J. Catino, I. King

All Dugoni School of Dentistry Faculty Articles

Interleukin-10 (IL-10) is a recently described pleiotropic cytokine secreted mainly by type 2 helper T cells. Previous studies have shown that IL-10 suppresses cytokine expression by natural killer (NK) and type 1 T cells, thus down-regulating cell-mediated immunity and stimulating humoral responses. We here report that injected IL-10 protein is an efficient inhibitor of tumor metastasis in experimental (B16-F10) and spontaneous (M27 and Lox human melanoma) metastasis models in vivo at doses that do not have toxic effects on normal or cancer cells. Histological characterization after IL-10 treatment confirmed the absence of CD8+ and CD4+ T cells and macrophages at …

Outdoor Living Barn: A Specialized Windbreak, Kris Irwin, Jerry Bratton

Agroforestry Notes (USDA-NAC)

In April of 1987, a spring blizzard swept through northern Kansas and southern Nebraska killing nearly 60,000 newborn calves and other winter stressed animals. This tremendous loss could have been lessened had protection, such as outdoor living barns (OLB), been provided to reduce the windchill. An outdoor living barn is a specialized windbreak, typically composed of trees and shrubs, and strategically located in open grasslands, center pivot irrigation corners, and pasture areas to protect livestock during severe weather situations.

The purpose of an OLB is to: 1) defuse and deflect cold winds away from livestock, moderating the windchill; and 2) …

Mr400: Assessing Compliance With Bmps On Harvested Sites In Maine: Final Report, Russel D. Briggs, Alan J. Kimball, Janet Cormier

Miscellaneous Reports

The research was designed to help answer three questions: (1) What are the documented types of impacts on water quality from forestry activities in Maine? (2) Do timber harvesters use the best management practices recommended by the state to control water pollution? (3) Are these practices effective when they are used?

Lupin Logic Number 73

Lupin Logic

Contents

Lupins in Chile Erik von Baer

- 1995-96 season

- Outlook for 1996/97

- The future

CMV resitance in yellow lupins

Aphid outlook

Lupin market

Pulse conference

The First Step In The Case For Great Ape Equality: The Argument For Other Minds, Kristin Andrews

Psychology Collection

No abstract provided.

Indicators For Determining The Sources And Extent Of Fecal Contamination In Coastal Waters: An Annotated Bibliography, Donald S. Mccorquodale Jr., Curtis M. Burney, Molly Row

Marine & Environmental Sciences Faculty Reports

No abstract provided.

Pollen Dispersal In Low-Density Populations Of Three Neotropical Tree Species, Jason D. Nason, E. A. Stacy, J. L. Hamrick, S. P. Hubbell, R. B. Foster, R. Condit

Jason D Nason

Studies of mating patterns of tropical trees, typically involving common species, have revealed that most species are outcrossed and that, in some cases, a significant reaction of outcross pollen moves long distances. We evaluated mating systems and effective pollen dispersal for three hermaphroditic insect-pollinated Neotropical tree species, Calophyllum longifolium, Spondias mombin and Turpinia occidentalis, all of which occurred at low adult densities at the study site. Mating patterns were estimated for each maternal tree within 84-ha populations of C. Longifoliuman d S. mombin in 1992 and 1993 and within a 50-ha population of T. occidentalis in 1993. Each population was …

Osteology, Morphology, And Female Choice In Species Of The Genus Cynolebias (Cyprinodontiformes: Rivulidae), Marcelo Loureiro Barrella

Master's Theses

Relationships among the species of the annual fish Cynolebias are unclear. An analysis of the utility of different osteological and morphological characters for phylogenetic analyses was done using clear and double stained specimens and measurements of morphometric parameters respectively. Scanning electron microscopy was used to study the morphology of the egg chorion. Female choice tests were also conducted to test male traits that may be preferred by females. The osteological analysis showed that some characters previously unreported may be phylogenetically useful. Head scalation patterns were found to be intraspecifically variable, making them inappropriate to use in systematics. Each morphometric parameter …

The Effect Of Thyroid Hormone On The Expression Of A7 Myosin Heavy Chain Mrna In Xenopus Laevis, Bradley Thomas Butkovich

Master's Theses

It has long been known that thyroid hormone (TH) is responsible for metamorphosis in tadpoles. It is also known that A7 MHC, an adult muscle isoform, is expressed in adult Xenopus laevis frogs, but not in larvae. Furthermore, the appearance of A7 corresponds with the onset of metamorphosis. The present study investigated the relationship between TH and the expression of A7 MHC mRNA in Xenopus laevis. Isolated tails in culture and 6-n-propyl 2-thiouracil (PTU) treated tails were treated with TH and the presence of A7 mRNA analyzed with in-situ hybridization, and RT-PCR. It was found that TH caused substantial …

The Effects Of 2,4-Dichlorophenoxyacetic Acid On The Diazotrophic Soil Microbial Community, Denise M. Brown

Master's Theses

This study measures the effects of 2,4- dichlorophenoxyacetic acid (2,4-D) on growth and nitrogen fixation capabilities of the native microbial soil community during eight field trials conducted over an eight month period. After application of 2,4-D, soil samples were analyzed for enumeration of diazotrophic and general microbial communities, and for nitrogenase activity as measured by the acetylene reduction assay (ARA). Statistical analyses were conducted using Minitab. Results show that 2,4-D significantly stimulated growth of diazotrophic microbes in 87.5% of the trials and was found to have a stimulatory growth effect on the general microbes in 62.5% of the trials. A …

Feasibility Of Stocking Grass Carp (Ctenopharyngodon Idella; Cyprinidae) To Control Hydrilla (Hydrilla Verticillata; Hydrocharitaceae) In Lake Anna, Virginia, Paul Matthew Overton

Master's Theses

The goal of this study was to demonstrate the feasibility of using triploid ·Ctenopharvngodon idella (grass carp) to control Hydrilla verticillata in the Waste Heat Treatment Facility (WHTF) in Lake Anna, Virginia. The four objectives were to determine: the frequency of occurrences of grass carp over hydrilla; the proportions of hydrilla to other aquatic macrophytes in the guts of grass carp; effects of physical and chemical factors on movement of grass carp; and a theoretical stocking rate of grass carp for the WHTF. Frequency of occurrence of grass carp over hydrilla was calculated to be 84% and correlated with impoundment …

Research Needs For The Risk Assessment Of Health And Environmental Effects Of Endocrine Disruptors: A Report Of The Us Epa-Sponsored Workshop, Rj Kavlock, Gp Daston, C Derosa, P Fennercrisp, Le Gray, S Kaattari, Et Al

VIMS Articles

The hypothesis has been put forward that humans and wildlife species have suffered adverse health effects after exposure to endocrine-disrupting chemicals. Reported adverse effects include declines in populations, increases in cancers, and reduced reproductive function. The U.S. Environmental Protection Agency sponsored a workshop in April 1995 to bring together interested parties in an effort to identify research gaps related to this hypothesis and to establish priorities for future research activities. Approximately 90 invited participants were organized into work groups developed around the principal reported health effects-carcinogenesis, reproductive toxicity, neurotoxicity, and immunotoxicity--as well as along the risk assessment paradigm--hazard identification, dose-response …

Impact Of Pesticide Residues In Composted Lawn Waste Compost On Vegetable Crops [Abstract], Cindy Stuefer-Powell, Patrick J. Shea, Laurie Hodges, Garald L. Horst

Department of Agronomy and Horticulture: Faculty Publications

To conserve space in the Lincoln (Nebraska) city landfill, a program for composting urban yard waste was initiated in 1992.

Vif-Negative Human Immunodeficiency Virus Type 1 Persistently Replicates In Primary Macrophages, Producing Attenuated Progeny Virus, Iqbal Chowdhury, Wei Chao, Mary Jane Potash, Pavel Sova, Howard Gendelman, David J. Volsky

Nebraska Center for Virology: Faculty Publications

The vif gene of human immunodeficiency virus type 1 (HIV-1) is required for efficient infection of primary T lymphocytes. In this study, we investigated in detail the role of vif in productive infection of primary monocyte-derived macrophages (MDM). Viruses carrying missense or deletion mutations in vif were constructed on the background of the monocytotropic recombinant NLHXADA-GP. Using MDM from multiple donors, we found that vif mutants produced in complementing or partially complementing cell lines were approximately 10% as infectious as wild-type virus when assayed for incomplete, complete, and circularized viral DNA molecules by quantitative PCR amplification or for viral core …

Density-Dependent Settler-Recruit-Juvenile Relationships In Blue Crabs, Aj Pile, R Lipcius, J Van Montfrans, R J. Orth

VIMS Articles

Current theory on the population dynamics of marine species with complex life history patterns posits that a suite of physical and biotic forces (e.g., habitat structure and density-dependent predation or emigration) control survival and abundance in early life history, particularly after settlement. We have conducted a long-term sampling effort accompanied by a series of field and laboratory experiments examining the joint effects of habitat type, body size, and population density upon abundance and survival of early juveniles of the blue crab, Callinectes sapidus. In addition, the chance occurrence of a tropical storm during one set of experiments provided an opportunity …

Kentucky Warbler (Vol. 72, No. 3), Kentucky Library Research Collections

Kentucky Warbler

No abstract provided.

An Animal Model For Development Of Pulpal Inflammation, John W. Masters

Loma Linda University Electronic Theses, Dissertations & Projects

Several animal models have been proposed to develop pulpal inflammation. Procedures recommended in these models have many variables and have not produced consistent results. The purpose of this study was to create pulpal inflammation using the Nd:YAG laser.

Forty-five cuspids of 12-18 month old beagle dogs were lased at 5, 7.5, and 10 watts (W) for 20 seconds with a 600 micron contact probe. Five cuspids were not lased but were used as controls. Following laser application, the teeth were extracted at intervals of 3, 7, and 30 days. They were then fixed in 10% formalin, decalcified, sectioned, and stained …

Nebline, August 1996

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

I Care For My Animals
The garden fence
August garden hints
An easy way to dry flowers
Harvesting fruits and vegetables
The Herb Garden
Hungry hoards of hoppers, again!
Recycle your pesticide containers at the 1996 Lancaster County Fair!
Special field day and tour on specialty crops
August plantings of alfalfa and grass
Grazing drought-stressed pasture
Bug bites
Cicada killers are common now
Bug zappers—good, bad, electrifying?
Let’s manufacture monster bubbles!
EnviroShorts
Yow! Watch out for yellow jackets!
Watersheds & water quality
New items accepted at recycling sites
Ants are public enemy #1
Nebraska State 4-H Camp NatureLink weekend
Family …

Ard News August 1996

Agricultural Research Division: News and Annual Reports

CONTENTS:

COMMENTS FROM THE ASSOCIATE DEAN
ARDC SUPERFUND UPDATE
WIDAMAN TRUST DISTINGUISHED GRADUATE ASSISTANT AWARD
HARDIN DISTINGUISHED GRADUATE FELLOWSHIP FOR 1996-1997
INNOVATIVE AND HIGH RISK RESEARCH PROGRAM
BURLINGTON NORTHERN ENDOWMENT
NATIONAL RESEARCH INITIATIVE
PROPOSALS SUBMITTED FOR FEDERAL GRANTS
NEW OR REVISED PROJECTS
PROJECTS APPROVED BY THE COMMODITY BOARDS
GRANTS AND CONTRACTS RECEIVED JUNE AND JULY, 1996

A Protocol For Ecosystem Management, Jack A. Stanford, G. C. Poole

Biological Sciences Faculty Publications

No abstract provided.

Abiotic Stress And The Relative Importance Of Interference And Facilitation In Montane Bunchgrass Communities In Western Montana, John T. Greenlee, Ragan M. Callaway

Biological Sciences Faculty Publications

No abstract provided.