Life Sciences Commons^™

In Vivo Evaluation Of Microfibrous Bioactive Borate Glasses For Use In Wound Healing, Yinan Lin

Masters Theses

"This project consisted of two parts focused on in vivo evaluation of microfibrous bioactive borate glasses for use in wound healing. Part one of the project involved subcutaneous implantation of two types of borate glass microfibers and 45S5 glass micro fibers in the dorsum of rats and histologically evaluating the soft tissues at the implant sites at 2 to 4 weeks post surgery. The primary objective was to assess the angiogenic response to the implanted materials. The 13-93B3 and 13-93B3Cu glass microfibers were found to significantly increase the microvascular density in soft tissue, an outcome that suggests these simple and …

Ancient Mitochondrial Dna From Pre-Historic Southeastern Europe: The Presence Of East Eurasian Haplogroups Provides Evidence Of Interactions With South Siberians Across The Central Asian Steppe Belt, Jeremy R. Newton

Masters Theses

Studies of mitochondrial DNA (mtDNA) polymorphism have provided valuable insights for understanding patterns of human migration and interaction. The ability to recover ancient mtDNA sequence data from post-mortem bone and tissue samples allows us to view snapshots of historic gene pools firsthand, provided that great care is taken to prevent sample contamination. In this study, we analyzed the DNA sequence of the first hypervariable segment (HVSI) of the mtDNA control region, as well as a portion of the coding region, in 14 individuals from three collective burials from the Neolithic Dnieper-Donetz culture and three individuals from Bronze Age Kurgan burials, …

Study Of Metamerism And Inconstancy Of Basic Color System Used In Wood Grain Laminates, Awadhoot Vijaykant Shendye

Masters Theses

In product gravure printing, many spot color inks are used. Because the use of spot colors tends to be customer specific, they are typically mixed in house by the gravure printer to satisfy the requirements of a specific customer order. Printers are often concerned about the best utilization of inks, and their recyclability, if possible, to make the production more sustainable. Spot colors may exhibit metameric behavior. This tendency causes problems in color matching, as these products are viewed in a standardized environment, using light source D₅₀ or D₆₅ , but are viewed under fluorescent lighting at the …

Social Dimensions Of Lake Ecology: Stakeholder Perception Surveys Used To Design Effective Lake Management Plans, Laurie Nannini

Masters Theses

Lake conservation management plans have exhibited varying levels of success through out Illinois. Recent research, in eutrophic lakes shows that pollution at any level stabilizes when stakeholder cooperation is enhanced by high conformist tendency and social concern. Involving stakeholders in preliminary stages of lake management plans creates more legitimacy and connectedness with the local government as well as stakeholder involvement with management plans. Public opinion data also allows policy makers to sustainably involve stakeholders in management plans. Members of the Illinois Lake Management Association were surveyed on their perceived threats to lakes, preferences on lake facilities and activities. Responses were …

Is Anthropogenic Habitat Change The Driving Force Of Rapid Evolution Of Southeastern U.S. Coastal Deer Populations?, Nicole L. Storm

Masters Theses

Many factors, such as genetics, food availability and the exact food species, can affect the growth rate and asymptotic size of animals. Since the I 960's, the coastal areas and islands in Georgia, North Carolina, and South Carolina have become vacation destinations with large and lavish resort communities built to accommodate the large number of visitors to areas that were once called home by just a small number of residents. These developments brought with them non-native plants and improved soil quality for both native and non-native plants. During this same time period, the whitetailed deer ( Odocoileus virginianus) native to …

Mutation Of The Hof1 Pest Domain Affects Cytokinesis In Budding Yeast, Katherine E. Stockstill

Masters Theses

"Cytokinesis is the division of a cell into two daughter cells. Cytokinesis failure results in polyploidy, which may contribute to tumorigenesis or cause cell death. The Saccharomyces cerevisiae protein Hof1, which is required for efficient cytokinesis, is phosphorylated during mitosis. The hypothesis that MEN (mitotic exit network) dependent phosphorylation regulates Hof1 degradation, triggering completion of cytokinesis was tested. Hof1 contains a PEST sequence, which is rich in proline, glutamic acid, serine, and threonine, and is believed to be involved in protein degradation. To test the hypothesis, we created two non-phosphorylatable mutants of the Hof1 PEST domain tagged with GFP. The …

Analysis Of Extinction Of Fibroblast-Specific Gene Expression In Somatic Hepatic Hybrids Using Cdna Microarray Technology, Sushma K. Shrestha

Masters Theses

Extinction of tissue-specific genes in mammalian somatic cell hybrids is a well known epigenetic phenomenon and knowledge of this process is useful in understanding molecular mechanisms underlying tissue specific gene expression. Signaling proteins critical for the activation of transcription including transcription factors, co-activators, and repressors, play a role in the establishment and maintenance of tissue-specific phenotypes. Tissue-specific transcription factors are the key driving force of tissuespecific gene expression. However, mechanisms responsible for gene silencing are largely unknown.

Extinction of liver specific traits in somatic hybrids generated by the fusion of hepatoma and fibroblast cells has been well documented and is …

Interactions Of Diet And Behavior In A Death-Feigning Snake (Heterodon Nasicus), Andrew Michael Durso

Masters Theses

Studies of animal behavior in captivity are limited in their ability to explain the influence of a natural environment on behavioral ecology. Defensive behaviors vary among individual animals, between sexes and with age, as well as with other less well-known factors. The toxin-rich diet of many toad-eating snakes might enable or cause their passive terminal defensive behavior of death-feigning. I videotaped death-feigning behavior in wild Plains Hog-Nosed Snakes (Heterodon nasicus) subjected to standardized harassment. Using stable isotopes, I also described the diet of the same individuals subjected to behavioral analysis (n = 40). Heterodon had broader diets than other snakes …

Estrogen Promotes Neurite Outgrowth In Olfactory Epithelial Explant Cultures Through The Estrogen Receptor, Apryl E. Pooley

Masters Theses

No abstract provided.

A Functional Genomics Approach To Analyze Two Methionine-[Gamma]-Lyase Orthologs In "Ferroplasma Acidarmanus" Using In Vitro Enzyme Assays, Md. A. Wadud Khan

Masters Theses

Functional genomics predicts that ferl genome has two candidate genes for the production of two methionine-y-lyase (MGL) orthologs, which are thought to be pivotal for the production of methanethiol. These orthologs show a high level of protein sequence identity ( ~ 3 5%) and similarity (~60%) when compared to known MGLs. In addition, ClustalW2 alignment of ferl MGL orthologs and other known MG Ls showed a high level of similarity in amino acid residues, which are involved for the stabilization of 3D structure of MGL and in forming active site for the substrates. To confirm the predictive functions of these …

The Evolution Of Leaf Anatomy In Badiera (Polygalaceae), Laura Brauer

Masters Theses

Leaf samples from Polygalaceae: nine species of Badiera, two species of Bredemeyera, three species of Hebecarpa, and two species of Polygala were taken from herbarium specimens for anatomical observations. Several methods of rehydration were compared to a control of fresh material fixed in the field for four species of Badiera. Soaking in concentrated ammonium hydroxide proved to be the best overall means of rehydrating leaves of the genus Badiera and related genera. All leaves were embedded in paraffin for sectioning and cleared in 5% aqueous sodium hydroxide for examining epidermal features. Twelve anatomical characters were mapped onto a molecular phylogeny …

Isolation And Characterization Of Bacterial Symbionts From Crotalaria Spectabilis Grown On Trichloroethene Contaminated Soil, April Lyn Rocha

Masters Theses

"The discovery of several members of the Leguminosae family growing on a TCE contaminated site demonstrated that they have the capabilities to tolerate the TCE contamination. This led to an investigation of the plants' ability to metabolize TCE. Research by Dr. Lee Newman showed that nodules from Crotalaria spectabilis grown on the site had TCE metabolites present while nodules from other legumes at the site did not. The presence of TCE metabolites within the C. spectabilis nodules suggests that the bacterial symbiont may be responsible for TCE metabolism. This led us to investigate the symbiont of C. spectabilis and its …

Synthesis And Toxicity Studies Of Imidazolium-Based Ionic Liquids, Ninu Madria

Masters Theses

"There is a considerable recent interest in the applications of room temperature ionic liquids (RTILs) as green solvents in organic synthesis and as nonvolatile electrolytes in lithium ion batteries. A variety of commercially available imidazolium based RTILs have been used for these applications, mainly due to their negligible vapor pressures, high thermal stabilities, wide liquid range, wide electrochemical windows and high anodic stability. The state of the art lithium ion batteries require the use of electrolytes that have relatively low viscosities at temperatures as low as -35°C. This thesis describes the synthesis of novel oxygenated and fluorinated imidazolium based RTILs …

Bat Habitat Use And Roost Tree Selection For Northern Long-Eared Myotis (Myotis Septentrionalis) In North-Central Ohio, Timothy J. Krynak

Masters Theses

Understanding roosting behavior and habitat use of bats is an important component when unraveling life histories and their ecology. Ohio remains under-represented in published information of bats compared to surrounding states. This large scale survey in 2002–2003 and Myotis septentrionalis radio telemetry study in 2005 is one of the few conducted within the state and is the first in Ohio’s North-Central region. It is also the first study conducted where net sites were chosen randomly and spatially distributed to adequately survey bat populations within Cuyahoga Valley National Park and Cleveland Metroparks. This survey documented seven species (n = 668), with …

The Seasonality Of Parasites In Illinois House Sparrows (Passer Domesticus): Effect Of Stress On Infection Parameters, Tiffany C.M. Gibson

Masters Theses

Parasitism often shows seasonal variation in their hosts and has been associated with host densities, host stress levels or transmission strategies (Moller et al 2003, Andersen and May 1982 ). This study was designed to evaluate the role of direct versus indirect life cycle transmission strategies, using Isospora and haemosporidians; and the impact of proposed ecological (winter) and physiological (breeding) stresses on parasitism levels. Coccidia have direct life cycles and infect gut tissues and are passed by fecal-oral transmission. Haemosporidians are blood parasites with an indirect life cycle that requires blood-feeding insects for transmission. If parasitemias are influenced by host …

Multiple Endpoints Of Endocrine Disruption In Gravid Watersnakes (Colubridae:Nerodia) As A Function Of Ingestion Of A Common Herbicide, Lorin Anne Neuman-Lee

Masters Theses

Ecotoxicological studies that focus on a single endpoint might not accurately represent the true ecological effects of a contaminant. Exposure to atrazine, a widelyused herbicide, disrupts endocrine function and sexual development in amphibians, but studies involving reptilian species are lacking. This study examines several effects of atrazine ingestion on gravid females and neonates exposed in utero of the Northern Watersnake (Nerodia sipedon). Twenty-five gravid N. sipedon were collected and kept in the lab for the entirety of their gestation period. Each snake received one of four doses of atrazine: control, 2, 20, or 200 ppb. Blood samples were drawn each …

A Spatially Explicit Model To Predict Radiocesium Body Burdens In White-Tailed Deer On The Department Of Energy's Savannah River Site, Christopher W. Bobryk

Masters Theses

A spatially explicit exposure model was developed to interpolate and predict radiocesium (137Cs) body burdens found in white-tailed deer (Odocoileus virginianus) on the U.S. Department of Energy's Savannah River Site (SRS) in west-central South Carolina. Since 1965 hunting has been allowed from permanent stands as a mechanism to manage the herd and all animals have been monitored in the field for gross beta/gamma activity levels providing a long-term spatially explicit dataset. Until now no study has attempted to model deer 137Cs at the hunt-stand level, but rather at the compartment level (1,000 - 5,200 ha). The models described here use …

The Spatial Distribution Of Japanese Beetles, Popillia Japonica, In Soybean Fields, Stacey A. Sara

Masters Theses

The Japanese beetle, Popilliajaponica Newman, is a serious pest of many agricultural and horticultural plants. Little is known about the distributions of Japanese beetles in agricultural fields which makes pest managyment more difficult. We studied the spatial distribution of Japanese be~tles in soybean fields. In particular, we examined how distance from an edge, edge direction, and edge type affected the distribution and abundance of beetles. Twenty-five soybean fields were transected parallel with the field edges at varying distances from the field edges and sampled for beetles. An edge effect for density was discovered; beetle numbers decreased significantly with increasing distance …

Prey Preference As A Function Of Feeding Experience And Prey Type In Neonate Gartersnakes (Colubridae: Thamnophis), Kathryn B. Hale

Masters Theses

Several species of potential prey have been introduced into the geographic range of the Two-striped Gartersnake, Thamnophis hammondii. Some of these prey species possess defenses that present novel challenges to the snake predator. Despite novel predator defense mechanisms (including secretions from skin glands), adult T. hammondii ingest several of these prey species without adverse effects. Although ~dult T hammondii are dietary generalists, almost nothing is known about prey preferences in newborn individuals that lack feeding experience. My study determined whether or not T. hammondii innately exhibits a preference among native and introduced prey types. Furthermore, I compared the degree of …

A Model Cost Analysis Comparing Solar, Wind And Geothermal Energies, Including Alternatives To Changing Energy Sources, Using Texas Households And Weather, Kimberly Speed

Masters Theses

The Emergency Economic Stabilization Act of2008 (P.L. 110-343) provides several incentives and tax credits for Americans interested in investing in alternative energy resources. Solar, wind, and. geothermal systems receive the most tax credits due to their low carbon output, but a determination must be made as to which resource is the most accessible and economically feasible. The State of Texas was used as a model for comparing the three renewable energy resources through analysis of weather variables, geological features, resource availability, amount of energy harvested in relation to equipment, and installation and maintenance costs.

All three energy systems have positive …

Analysis Of Eurycea Hybrid Zone In Eastern Missouri, Bonnie Jean Beasley

Masters Theses

"Evolutionary mechanisms are often difficult to observe in action because evolution generally works slowly over time. Hybrid zones provide a unique opportunity to observe many evolutionary processes, such as reinforcement, because of the rapid changes that tend to occur in these zones. Salamanders provide an ideal model for examining the rapid changes in populations that result from hybridization because many closely-related species lack reproductive barriers. In Missouri, a well-documented hybridization zone exists among the two subspecies Eurycea longicauda longicauda (long-tailed salamander) and E. L. melanopleura (dark-sided salamander). These salamanders inhabit caves, limestone creek beds, and abandoned mine shafts. A closely …

The Capacity Of Diatom Species To Survive Ingestion By The Algivorous Minnow, Pimephales Notatus, Paul Gregory Grubach

Masters Theses

The capacity of diatom species to survive gastrointestinal passage through the algivorous minnows Pimephales notatusand Campostoma anomalum was studied. From a site on the Grand River in northeastern Ohio, 27 minnows and 7 epilithic diatom samples were taken. In order to determine whether diatom taxa varied in digestibility, live/dead ratios of diatom cells taken from the minnows’ feces were compared with live/dead ratios of cells taken from immersed rocks. Diatoms that were live/undigested at the time of collection were differentiated from dead/digested cells under light microscopy by noting the presence of chloroplasts and/or lipid droplets. Seventy-seven percent of the …

The Role Of Skeletal Development In Body Size Evolution Of Two North American Frogs, Sarah Beth Havens

Masters Theses

"In order to better understand the evolution of miniaturization in Acris blanchardi, a North American Hylid with a unique life history and of ecological interest in the United States. The development and ossification sequences of 48 larvae, 5 juveniles and 5 adult A. blanchardi were examined. The adult was described by Maglia et al. (2007) to be a miniature and to display morphological novelties. In addition, 44 larvae, 2 juvenile and 5 adults of Pseudacris crucifer a closely related frog that has been suggested to be a miniature, were examined. The cranial and postcranial adult elements of this species, as …

Zinc Oxide Nanoparticle Disruption Of Store-Operated Calcium Entry In A Muscarinic Receptor Signaling Pathway, Hsiu-Jen Wang

Masters Theses

"The influences of ZnO nanoparticles on cellular responses to activation of muscarinic receptors were studied in Chinese hamster ovary cells expressing the human M3 muscarinic acetylcholine receptor. ZnO particles (20 nm) induced cytotoxicity in a time and concentration-dependent manner: following a 24 h exposure, toxicity was minimal at concentrations below 20 µg/ml but virtually complete at concentrations above 28 µg/ml. ZnO particles did not affect antagonist binding to M3 receptors or allosteric ligand effects, but increased agonist binding affinity while eliminating guanine nucleotide sensitivity. At a noncytotoxic concentration (10 µg/ml), ZnO increased resting [Ca²⁺]i from 40 to 130 nM without …

Does Evolutionary Exposure Mediate Allelopathic Effects?, Nikki Leigh Pisula

Masters Theses

Allelopathy most commonly refers to chemically mediated plant interference, though the effects may range from beneficial to inhibitory. Allelopathy has been suggested as a potential mechanism for invasion success because invasive species may produce allelochemicals that are evolutionarily novel to recipient communities. To test for evolutionary mediation of allelopathy, six target species were grown in a factorial experiment with and without activated carbon or a native allelopathic species, Solidago canadensis (Canada goldenrod). I used activated carbon to remove allelochemicals to separate allelopathic effects from resource competition. The species represented pairs of evolutionary experienced (native - N) and evolutionarily na'ive (non-native …

Effects Of Forest Edges On Population Dynamics In A Successional System, Kimberly Anne Lang

Masters Theses

Abandoned agricultural land is often bound by remnant woodlands which may locally influence successional dynamics. The primary objective of this study was to examine the effect of an old-growth forest edge on plant population dynamics within a successional system. Data from the Buell-Small Succession Study (BSS) in New Jersey were used to analyze population dynamics in relation to the forest edge during the first 50 years of succession following agriculture. It was hypothesized that edge responses would be driven by either differential colonization (dispersal and establishment) or differential performance (increase within plots).

By looking at individual species' edge responses, I …

Soil Variability And Its Influence On Plant Performance, Jeremy R. Klass

Masters Theses

During recorded history, nearly all temperate deciduous forests in eastern North America have seen extensive agricultural land-use conversions. Persistent influences of agricultural disturbance continue to shape the diversity and composition of the plant community long after agriculture has ceased. While some measures of ecosystem functioning may recover relatively quickly from agricultural disturbance, other characteristics such as levels of organic matter, carbon, and phosphorus, may remain different from primary forests for much longer periods. The alteration of the original ecosystem due to agriculture practices has far reaching impacts on biotic and nutrient dynamics along with causing significant shifts in community composition …

Population Ecotoxicology Of The Common Snapping Turtle In Agriculturally Impacted Lotic Ecosystems, Derrick L. Douros

Masters Theses

Anthropogenic impacts on lotic systems, including pollutants, have had both direct and indirect negative effects on the aquatic habitats of the world. Goals of this study are: (1) Quantify atrazine, the atrazine metabolite diaminochlorotriazine (DACT), and glyphosate burdens in common snapping turtle (Chelydra serpentina) tissue from individuals collected within the Embarras River in Coles County, Illinois. (2) Quantify the atrazine, DACT, and glyphosate loads in water from the aquatic habitats in which common snapping turtles reside. (3) Quantify the relative abundance of all turtle species and movement patterns of common snapping turtles in this lotic ecosystem. Hoop nets and modified …

Laryngeal Apparatus And Call Structure In North American Hylids, Barbara Ann Catherine Fears

Masters Theses

"Although the ecological implications and structure of frog calls have been the subject of much study, little is known about the association between the laryngeal apparatus morphology and call structure in North American hylids. In this study linear measurements of the laryngeal apparatus were captured and compared to the call structures of thirteen species of North American hylids. Species examined included: Pseudacris crucifer, P. triseriata, P. ocularis, Acris crepitans blanchardi, Hyla avivoca, H. cinerea, H. gratiosa, H. chrysoscelis, H. versicolor, H. squirella, H. femoralis, H. arenicolor, and H. eximia. Six homologous landmark points were identified, and the lengths between them …

Fish Assemblages In Manistee River Tributaries: Longitudinal Distribution Analysis, Seasonal Variation, And Riparian Improvement Evaluation, Nicholas J. Gressick

Masters Theses

Sedimentation affects both stream physical and biological integrity. Improperly designed stream passage accompanied with sedimentation and altered hydrology can impede fish passage and reduce fish assemblage integrity. The purpose of this study was to: 1) quantify impacts of poorly constructed road stream crossings and eroding banks on fish assemblages, and 2) assess these sites as sediment sources and connectivity breaks on entire fish assemblages and individual fish species. Electrofishing was conducted during spring and fall 2004 and 2005. A total of 29 electrofishing reaches were sampled which included 5 road-stream and streambank restoration sites. Sickle Creek (1st order) had reduced …