Life Sciences Commons^™

A Community Ecology And Stable Isotope Study Of Plankton, Macroinvertebrate, And Fish Communities Surrounding Groundwater Vents In Lake Huron, Michigan, Thomas Garrison Sanders Jr.

Masters Theses

Extreme environments can create ecotones with uncharacteristic physicochemical conditions and ecological communities. Recently, several submerged sinkholes were discovered in Lake Huron where hypoxic groundwater that is rich with dissolved ions intrudes into surrounding lake water. Researchers have documented large growths of cyanobacteria and chemosynthetic bacteria surrounding these groundwater vents, but little is known if this production is utilized by upper trophic level organisms and how the environmental conditions affect local community density and diversity. My objectives were threefold: (1) I wanted to confirm the presence and describe the nature and extent of chemical and physical gradients in the sublacustrine sinkhole …

From In-Vitro To In-Vivo: Corporate Development And Efficacy Of A Topical Hair Growth Agent Derived From Natural Extracts, Kelly Michael Glynn

Masters Theses

Androgenetic alopecia (male pattern baldness) affects up to 50% of the world’s population, propelling the development for a possible treatment. The hair follicle is influenced by several genetic and physiologic factors, which, when gone awry, lead to androgenetic alopecia. Vascular endothelial and keratinocyte growth factors are believed to be promoters of hair growth, as is inhibition of the proteasome complex. The cytokine IL-1α is also known to regulate follicle dynamics. The research objective described herein was an attempt to develop a botanical blend, which could mediate the above biomarkers, be successfully incorporated into a safe topical product and be evaluated …

Sediment Remediation Impacts On Macroinvertebrate Community Structure: Assessing The Success Of Urban Stream Restoration, Laurie Beth Nederveld

Masters Theses

Land use practices altering the natural landscape have resulted in the widespread degradation of stream ecosystems and the need for urban stream restorations. While a number of studies have evaluated the success of these stream restoration efforts, few have assessed the recovery of macroinvertebrate communities following the remediation of contaminated sediments. The purpose of my study was to evaluate the impact of sediment remediation activities on macroinvertebate abundance, diversity, and richness to determine the success of stream restoration in Ruddiman Creek, a small stream in the Muskegon Lake watershed. During my investigation, macroinvertebrate samples were collected from all available habitat …

Oxidative Stress, Calcium Homeostasis, And Altered Gene Expression In Human Lung Epithelial Cells Exposed To Zno Nanoparticles, Chuan-Chin Huang

Masters Theses

"The influence of 20 nm ZnO nanoparticles on oxidative stress, intracellular calcium homeostasis, and gene expression was studied in human bronchial epithelial cells (BEAS-2B). ZnO caused a concentration- and time-dependent cytotoxicity while elevating oxidative stress and causing membrane damage (cellular LDH release). There was a remarkably steep relationship between concentration and toxicity at concentrations from 5 to 10 μg/ml. Exposure to ZnO increased intracellular calcium levels in a concentration- and time-dependent manner. Treatment with the antioxidant N-acetylcysteine prevented cell loss and diminished the increase in intracellular calcium concentration, suggesting oxidative stress mediated cytotoxicity. Exposure to a sublethal concentration of ZnO …

The Effect Of Coalmine Effluent On The Parasite Assemblages Of Lepomis Spp. In The Saline River Basin, Andrew Claxton

Masters Theses

To determine the efficacy of using parasite assemblages as bioindicators of coalmine effluent, a survey of gastro-intestinal helminths from the gut of sunfish, Lepomis spp, was performed. Five species of Lepomis, L. cyanellus, L. nwcrochirus, L. megalotis, L. microlophus, and L. gulosus, were collected from six locations near the Palzo mine, an abandoned coalmine that actively discharges into the lower reach of Sugar Creek. Sunfish were collected from three sites which did not receive effluent from the Palzo mine (Sugar Creek (37° 37'/ 88° 50'), South Fork(37° 41 '/ 88° 52'), and the Little Saline River (37° 38'/ 88° 38')) …

Feathers As Bioindicators Of Pcb Exposure In Clapper Rails, Jay W. Summers

Masters Theses

In this study we used feathers as a biomonitor for exposure to the polychlorinated biphenyl (PCB) Aroclor 1268. Clapper rails have been used as an indicator species of environmental damage for the LCP superfund site located in Brunswick, GA USA. LCP is contaminated with Aroclor 1268, which has been used in limited amounts elsewhere and therefore can be used as a contaminant marker. Aroclor 1268, including congener profiles, were quantified using gas chromatography (GC). Concurrently each sample was quantified for total Aroclor 1268 using an enzyme-linked immunosorbant assay (ELISA) and compared to the GC results to determine if ELISA was …

Behavioral Response Of Japanese Beetles (Popillia Japonica) To Sex Pheromone: Exploring Factors Of Social Situation And Recent Mating Experience, Carissa A. Schoenick

Masters Theses

Insects in have several methods of advertising for mates; one important and common method is via the use of chemical pheromones. Insects release pheromones into the environment to communicate information about their sex, mating status and receptivity to others. For example, Japanese beetles (Popillia japonica) produce a very volatile sex pheromone conspecifics can readily detect. This pheromone is released by virgin females only, and triggers scramble competition among the males that detect and follow the signal for access to these females. Because Japanese beetles are an invasive species and an important pest of ornamental and agricultural plants, there is strong …

Photopigments As Descriptors Of Phytoplankton Assemblages For Biotic Assessment Of Illinois Lakes And Reservoirs: An Hplc Aided Analysis, Robert J. Krenz Iii

Masters Theses

The Illinois Environmental Protection Agency (IEPA) and U.S. Environmental Protection Agency (USEP A) identify needs to monitor aquatic resources by quantitative means using biocriteria in conjunction with routine environmental assessment to identify sources of impairment, and determine the effectiveness of management protocols (IEP A 2007; USEPA 2007). Though bioassessment protocols have been implemented within lotic systems and lakes of natural origin, investigation of their application to functionally unique reservoir habitat, which dominates the Midwest, has been limited. This study .evaluated the utility of photopigment profiles, attained using high-performance liquid chromatography (HPLC), for development ofbioassessment protocols for Illinois lakes and reservoirs. …

Nona-Arginine Peptides Facilitate Cellular Entry Of Semiconductor Nanocrystals: Mechanisms Of Uptake, Yi Xu

Masters Theses

"Luminescent semiconductor quantum dots (QDs) have recently been used for delivering and monitoring biomolecules, such as drugs and proteins. However, QDs alone have a very low efficiency of transport across the plasma membrane. In order to increase the efficiency of QD delivery, synthetic nona-arginine (sR9) was used, a cell penetrating peptide, to facilitate uptake. Data demonstrated that sR9 could significantly increase the cellular uptake of QDs by noncovalent binding between QDs and sR9. Furthermore, the mechanisms of QD/sR9 cellular internalization were investigated. Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/sR9, indicating that internalization is an energy-dependent process. …

Electricity Generation And Ethanol Production Using Iron-Reducing, Haloalkaliphilic Bacteria, Varun Paul

Masters Theses

"Microbial life in extreme environments has been studied primarily for their metabolic activities. Very few commercial or industrial applications have been known from these systems. In this study, the metabolic pathways and properties of bacteria from a haloalkaliphilic environment of Soap Lake, Washington were employed in two research applications related to energy production. In the first study, the bacterial cultures that were known to reduce iron (III) were used in a Microbial Fuel Cell (MFC) reactor. Iron reducing bacteria have shown to shunt their electrons on to the surface of a carbon electrode of an MFC, and the electrons can …

Dynamics Of Aggregation Formation In Japanese Beetles, Popillia Japonica, Katelyn A. Kowles

Masters Theses

For most insect aggregations to form, they need to be started by an initial individual (the pioneer) andjoined by later individuals (the joiners). Pioneers and joiners may differ with regard to characteristics such as sex and body size. We carried out three field experiments to examine the characteristics of Japanese beetles, Popillia japonica, pioneering and joining aggregations on host plants. Individual beetles were captured as they arrived on uninhabited grape plants, as well as plants designed to simulate aggregations with model beetles and feeding damage. For all experiments and treatments, the beetles arriving were significantly female-biased, with pioneer females having …

Ecology And Impacts Of Lianas In Regenerating Forests, Laura Marie Ladwig

Masters Theses

Although they are important components of forest communities, the general ecology of temperate lianas, especially during forest regeneration, is largely unknown. The dependence of lianas on other plants for physical support makes them a potentially important driver of community dynamics. I examined current and past vegetation data from an old-field succession study in the Piedmont region of New Jersey, USA to determine the dynamics of liana expansion and their role in forest regeneration. Fifty years of vegetation data were utilized to determine community controls and population dynamics oflianas during expansion. Five lianas, Celastrus orbiculatus, Lonicera japonica, Parthenocissus quinquefolia, Toxicodendron radicans, …

Aging In The Mammalian Olfactory System, Stephen Clark

Masters Theses

The purpose of this investigation was to investigate genetic and age~related differences in the mammalian olfactory system. The molecule of interest is apolipoprotein E (apoE), a component oflipoprotein molecules which functions in the transport of cell membrane components (triglycerides, lipids, and cholesterol). Specifically in the nervous system, apoE is synthesized by glial cells that function in recycling dead nervous tissue components for delivery to growing or dividing neurons. ApoE is present in three protein isofon11s: apoE2, which has been associated with type III hyperlipoproteinemia; apoE3, rhe normal human isoforrn; and apoE4, which has been associated with Alzheimer's disease (AD). The …

Interaction Of Apoe With Estrogen In The Olfactory System During Nerve Maintenance And Recovery, Michael M. Tonsor Ii

Masters Theses

Estrogen replacement profoundly aids in nerve regeneration after injury in adult mammalian models. Estrogen replacement also protects against olfactory dysfunction in mammals. The mechanism underly1ng estrogen's neuroprotective effects remains ambiguous. My goal is to investigate and better clarify if apolipoprotein E (apoE ), a lipid transporting protein, is a critical intermediary to estrogen's effects on nerve maintenance and regeneration.

My hypothesis is that apoE is a mediator of estrogen's beneficial effects. This hypothesis is based on the following observations from previous studies. First, olfactory nerve regeneration was delayed in apoE-gene knockout (KO) mice as compared to wild type (WT) mice. …

Fragmentation Effects On Fitness In Five Common Prairie Species, Lydia Miramontes Loyd

Masters Theses

Midwest tallgrass prairies have suffered extensive habitat loss and as a result persist as isolated and highly fragmented remnants. In such small, isolated remnants, genetic drift and inbreeding depression can lead to a loss of genetic variation over time. Reduced genetic variation is often associated with reduced levels of growth, survival, and reproduction as well as increased susceptibility to stress. This study examined fitness traits of five common prairie species to determine whether plants in small fragmented remnants had reduced fitness as compared to those in a larger remnant. Five prairie species were chosen for this study based on their …

Nest Success, Nesting Habitat, & Nestling Feeding Rates Of Red-Headed Woodpeckers In East-Central Illinois, Nathan Cole Hudson

Masters Theses

The Red-headed Woodpecker (Melanerpes erythrocephalus) is a classic oak savanna species that has suffered significant population declines caused, in part, by extensive loss of savanna habitat and agricultural development. Despite consistent data that reveal a dramatic decline throughout much of their range, few studies have examined other possible explanations. In an effort to determine potential causes for their continued decline (as well as add to existing knowledge of nesting biology), we examined nest success and nesting micro-habitat features. We compared micro-habitat variables at: 1) golf and non-golf course sites, 2) successful and failed nest sites, and 3) nest and random …

Analysis Of Transcription Factor Promoter Binding In Rat Hepatoma/Fibroblast Hybrids, Jordan C. Angle

Masters Theses

When hepatomas (liver cancer cells) are fused with fibroblasts (undifferentiated body cells), there is complete extinguishing of all tissue-specific gene expression. Once it was discovered that many hybrid cell lines do not express needed transcription factors for liver-specific gene transcription, the factors were reintroduced and forced to express through the use of plasmids. Even if the two primary transcription factors were present in the system, downstream tissue-specific gene expression was not rescued. To examine potential mechanisms for the repression of liver-specific gene expression in hepatoma x fibroblast hybrid cells, chromatin immunoprecipitation (ChlP) was used. This procedure allows the liver-specific gene …

Long-Term Assessment Of Predatory Fish Removal On A Pond-Breeding Amphibian Community In Central Illinois, Lee M. Gross

Masters Theses

Habitat loss and fragmentation are important contributors to population declines in many species, so maintaining suitable habitat has become a priority for wildlife management. Removal of introduced fish can help restore suitable habitats for amphibian populations, although long-term assessments of such removals are lacking at the community level. In 2001, drift fences and pitfall traps were constructed around the majority of four ponds to monitor an amphibian community in a nature preserve in central Illinois. Rotenone™ was applied to ponds containing introduced fish in 2001, and again, in 2003. I collected data from 2005-2007 to determine the long-term impacts of …

Habitat Selection And Niche Partitioning Of Fox And (Sciurus Niger) And Gray (Sciurus Carolinensis) Squirrels In Charleston, Il, Katelyn Marti

Masters Theses

Two species of tree squirrels inhabit Charleston, IL; fox squirrels (Sciurus niger) and gray squirrels (Sciurus caro/inensis). Although these species are sympatric throughout much of the eastern U.S., urban settings are usually occupied by one or the other. In the few localities where they co-occur, little is known about how they partition habitat. Therefore, the objectives of my study were to determine: (1) the relative abundance of each species, (2) whether habitat use differs between species, (3) if habitat use shifts with season, time of day, domestic predator abundance, or mast availability, and (3) observe interspecific and intraspecific interactions. The …

Survival Strategies Of The Endangered Physaria Ludoviciana (Silvery Bladderpod; Brassicaceae), Marissa Catherine Jernegan Grant

Masters Theses

Physaria ludoviciana (Nuttal) O'Kane and Al-Shehbaz is an endangered species in Illinois and Minnesota sand prairies, but has many individual populations in western states. Areas where P. ludoviciana occurs have high summer temperatures, a sandy soil with low water holding capacity and high drainage, the frequent disturbance of blowing sand and little canopy vegetation, giving it a full sun environment. This habitat is where few other plants are able to establish, providing P. ludoviciana with little competition. Physaria ludoviciana exists in a niche where it is adapted and able to establish and persist. The focus of this study was to …

In Vitro Evaluation Of Bioactive Glass Scaffolds And Modified Bioactive Glasses With An Osteogenic Cell Line, Vernon C. Modglin

Masters Theses

"This investigation consisted of two parts focused on bioactive glasses and scaffolds for in vitro growth of bone cells for tissue engineering and bone repair. The first part involved evaluation of the ability of two new types of porous 13-93 bioactive glass scaffolds to support the growth and differentiation of mouse MLO-AS cells, an established osteogenic cell line used as a model of developing bone tissue. The two scaffold types tested included 13-93 glass fiber and trabecular-like scaffolds seeded with MLO-AS cells and cultured for intervals of 2 to 12 days. Collectively, in vitro results indicate that the 13-93 glass …

Effect Of Calendering On Flexographically Printed Silver Flake Ink, Sujay Pandkar

Masters Theses

Interest in the printed electronics is increasing on a day-to-day basis. To utilize the concept of printed electronics on a wider range, printed circuit needs to perform more and more efficiently. The simplest printed circuit components include conductive traces, which can function as interconnects or contact electrodes. There are still some limitations to the conductivity of printed traces. Previous results showed an improvement in the conductivity of printed traces with a post cure treatment of 4 hours at 105°C. The present research explores a different approach towards improving conductivity using a post calendering treatment on-line.

The effects of calendering temperature, …

The Possibility Of Producing Biodiesel Fuel From Wood Pulping By Products, Yan Pu

Masters Theses

Biodiesel fuel produced from biomass material is a renewable and promising substitute fuel for petroleum diesel fuel, and it is relatively environmentally friendly. The Pulping process involves a huge amount of lignocellulosic materials. There may be a great potential of combining this fact with the biodiesel production, as well as the present biodiesel technologies.

This thesis focuses on the esterification reaction behavior of three main components of tall oil fatty acids, which are byproducts of paper manufacture. They are stearic acid, palmitic acid and oleic acid. Three levels of both catalyst and methanol were employed in the esterification for each …

Effect Of High Temperature Drying Of Paper On Heat Transfer Rates And Sheet Properties, Dania Awni Al-Said

Masters Theses

The objective of this research is to study the effect of high temperature on paper drying rates and properties of both virgin pulp and recycled paper. Process variables as shell temperature, moisture content, refining, basis weight and fiber recycling can affect the drying rate and paper properties. In addition, high surface temperature can affect the heat transfer rates from the dryer shell to the paper. The Gas Heated Paper Dryer (GHPD) is a high temperature dryer where internal shell temperature can reach 300° C, which significantly increases the drying rate. However, there are many concerns about the effect of this …

Community Response To Habitat Restoration In Sickle And Bear Creeks, With Emphasis On Mottled Sculpin In Sickle Creek, Jason Andrew Deboer

Masters Theses

Habitat restoration is employed by biologists and managers to improve the natural functionality and value of aquatic resources. Systems suffer impairment from many sources, including excessive fine sediment, which negatively affects substrate composition, channel morphology, aquatic invertebrate habitat, and fish reproduction and recruitment. Primary objectives included monitoring the biophysical response to sediment abatement in the Big Manistee River watershed. Secondary objectives included (1) placing the biophysical response to the restoration in the context of a much larger watershed plan, (2) quantifying seasonal mottled sculpin movement and habitat use in Sickle Creek for 1-year, and (3) determining habitat variables which may …

A Genetic Assessment Of The Red Squirrel In Illinois: Natives, Immigrants, Or Exotics?, William Beatty

Masters Theses

A small population ofred squirrels (Tamiasciurus hudsonicus) has existed in Iroquois County, Illinois since the 1970' s. The origin of this population is unknown, but some accounts suggest they are the offspring of released red squirrels from Minnesota. Alternatively, they may be a remnant Illinois population or more recent immigrants from Indiana. Finally, this population could reflect a mixed ancestry of the above scenarios. Mitochondrial DNA (mtDNA) was utilized to determine the genetic relationships among red squirrel populations in Illinois, Indiana, Michigan, Minnesota, and Wisconsin. My results showed low levels of haplotype diversity and nucleotide diversity in the Illinois sample. …

Beneficial Effects Of Estradiol Are Mediated Through Apoe, Aseem Hussain

Masters Theses

Estradiol is the primary premenopausal hormone circulating in women. It is a powerful hormone in the female body since it sends signals to the brain, regulates ovulation and menstrual cycle and acts on the nervous syste~ heart, breast and many other tissues in the body. However all estradiol production is stopped after menopause. Post menopausal women can develop a host of neurodegenerative diseases such as Alzheimer's disease. If the onset of progressive dementia is after menopause during loss of estradiol, it seems logical to assume that the lack of estradiol should have a big role to play in the pathogenesis …

Tracing Sperm In Multiply-Mated Female Anastrepha Suspensa, (Diptera:Tephridae), Preeti Dhakal

Masters Theses

Anastrepha suspensa females have four different sites of sperm storage in their reproductive tract, may mate with more than one male, and store sperm in differing amounts in each organ after copulation. In order to study the dynamics of sperm storage patterns and subsequent paternity outcomes, a method was developed for both genotyping and quantifying sperm in the spermathecae. Spermathecae were isolated and sonicated to remove maternal cells, and subsequently crushed between two microslides to release sperm from the capsule. After DNA isolation, microsatellite loci could be amplified by PCR for fragment analysis. A newly developed method was then used …

Artificial Riffles As A Stream Remediation Technique, Bethany S. Harrington

Masters Theses

No abstract provided.

Influence Of Seed Source On Vegetative And Reproductive Performance Of Three Common Prairie Grasses Common In Grassland Restoration, Matt Burmeister

Masters Theses

Though tallgrass prairie once covered 250 million acres of the midwestem U.S, now less than one percent· of this original area is left. In recent years the restoration and reconstruction of tallgrass prairie has become more popular throughout the prairie region. There is evidence that local and distant populations may both be practical seed sources for ecological restoration projects. To address this basic issue in restoration, I tested the how performance of three grass species from different locations was affected by the distance from the restored area. Three species, Andropogon gerardii, Sorghastrum nutans and Panicum virgatum, of perennial grasses common …