Life Sciences Commons^™

Effects Of Utilizing A Myofascial Foam Roll On Hamstring Flexibility, Elizabeth Sherer

Masters Theses

The purpose of this study was to identify if using a foam roller on the hamstring muscles would have positive effects and in tum increase hamstring flexibility. Hamstring injuries are prevalent in sports today. Lack of flexibility is seen as a possible cause for these injuries, so this study focused on a potential way of increasing hamstring flexibility. Self-induced myofascial release, using a foam roller, was the type of intervention used to try to have an effect on hamstring muscle flexibility. The use of a foam roller is becoming a popular tool utilized in many settings but there has been …

Cellular And Molecular Mechanisms Underlying The Interaction Of Apoe And Estrogen In Olfactory Neuronal Growth-Potential Relevance To Alzheimer's Disease, Minh Luong

Masters Theses

In recent years, there have been many studies looking at estrogen therapy (ET) as a treatment for Alzheimer's disease. Literature review suggests a close relationship between estrogen and apolipoprotein E (apoE) in the central nervous system. The mechanism underlying the interaction of apoE genotype with estrogen in the nervous system is not yet known. Therefore, the objectives of this research are 1) to determine the effects of estradiol treatment on basal cell proliferation and neuronal differentiation and 2) to identify the estrogen receptor subtype (ERα and ERβ) that mediates the increase of apoE levels, neurite outgrowth and neuronal numbers.

Results …

Survey Of Medicinal Herbs Of Central Illinois Prairie And Woodlands, Debra Ann Welch

Masters Theses

Illinois has been named the "Prairie State" for good reason. When the pioneers discovered a vast sea of grasses and forbs, they thought the prairie infertile. However, they soon found out that the blacksoil prairie was composed of a rich diversity of plant species that could provide much of the medical and nutritional needs of everyday life. The medicinal uses of many prairie species have not only been tested throughout history, but today modern science has also discovered their therapeutic importance.

The purpose of this study was to survey prairie species growing in the central Illinois counties of Coles and …

Molecular Mechanisms Involved In Anhydrobiosis Of Insect Cells, Diyagama Arachchi Ralalage Dilini Sewwandi Samarajeewa

Masters Theses

Animals possessing tolerance to extreme water stress are termed anhydrobiotes. Many desiccation tolerant organisms respond to water stress by intracellular accumulation of selected sugars such as trehalose and larger macromolecules such as Late Embryogenesis Abundant (LEA) proteins and thereby maintain the cell viability. Evidence indicates that the presence of trehalose and Late Embryogenesis Abundant (LEA) proteins may work synergistically to confer cellular protection during drying in eukaryotic cells. We evaluated any increase in cellular desiccation tolerance by expressing different LEA proteins in a non-desiccation tolerant insect cell line Drosophila melanogaster (fruit fly) (Kc167 cells) in the presence of trehalose.

Transgenic …

Broad-Spectrum Antibacterial Properties Of Metal-Ion Doped Borate Bioactive Glasses For Clinical Applications, Megan Ottomeyer

Masters Theses

"Bioactive glasses with antimicrobial properties can be implemented as coatings on medical devices and implants, as well as a treatment for tissue repair and prevention of common hospital-acquired infections such as MRSA A borate-containing glass, B3, is also undergoing clinical trials to assess wound-healing properties. The sensitivities of various bacteria to B3, B3-Ag, B3-Ga, and B3-I bioactive glasses were tested. In addition, the mechanism of action for the glasses was studied by spectroscopic enzyme kinetics experiments, Live-Dead staining fluorescence microscopy, and luminescence assays using two gene fusion strains of Escherichia coli.

It was found that gram-positive bacteria were more …

Early Arrest Mutant Ogre: A Defect In Cytokineis Caused By A Mutation In The Gene Racgap1, April Wicklund

Masters Theses

The early arrest mutant ogre, isolated in the Tübingen screen, has a block in cytokinesis (Kane et al., 1996). Here I use genetic and molecular techniques to demonstrate that the ogre phenotype is caused by a lesion in the gene encoding Racgap1. I performed fine mapping studies using microsatellite markers to a 0.15 cM region containing the ogre locus. DNA sequence data revealed a nonsense mutation in the Racgap1 gene of ogre mutants, and in situ hybridization experiments showed a loss of racgap1 mRNA at later stages of development, likely by nonsense mediated decay. This evidence supports the abolishment …

Laboratory And Field Performance Of The Biosand Point Of Use Water Filtration System In The Artibonite Valley, Haiti, Andrew John Sisson

Masters Theses

The research presented here is summary two years of studying the Biosand filter (BSF) both in Haiti during March 2011 and in the laboratory. In Chapter 2, we examined the long term use and sustainability 55 BSF systems near Deschapelles, Haiti and 47% were found to be no longer in use. Interviews with BSF owners revealed problems related to intermittent filter use. A review of 17 BSF field studies also was included to compare and substantiate observations made in Haiti. Together, previous field studies and our observations point toward the importance of providing culturally appropriate technologies and education materials explaining …

The Rise And Fall Of Kin Structure In The Neotropical, Family-Living Buff-Breasted Wren (Cantorchilus Leucotis) In Gamboa, Panama, Sarah C. Alessi

Masters Theses

Family-living species provide an exciting model to examine how natal dispersal and kin structure influence genetic structuring within local populations. Juvenile buff-breasted wrens (Cantorchilus leucotis) of both sexes delay dispersal and exhibit short-distance natal dispersal, which should lead to kin-structured populations in which relatives of both sexes occupy neighboring territories. Blood samples collected from juvenile and adult wrens in Gamboa, Panama were analyzed using microsatellite markers to determine whether related individuals are spatially clustered on neighboring territories, spatial clusters of relatives change over time, and if kin structure is sex-specific. Global and local spatial autocorrelation analyses detected genetic …

Origin Of Great Lakes Brown Trout, Salmo Trutta: A Phylogeographic Analysis Using Mtdna Sequence Variation, David Baisch

Masters Theses

The brown trout, Salmo trutta, was first introduced to the Laurentian Great Lakes in 1887 from European broodstocks to found a recreational salmonid fishery; however, the origins of these progenitor lineages remain largely unknown. Trout from these regions are very specialized to their native habitats and matching North American stocks to similar watersheds may help increase survivability by introducing the stock to a more appropriate environment. The objective of this study was to determine the European origins of brown trout found in the Great Lakes. We analyzed 144 brown trout from ten watersheds across Michigan and Wisconsin and identified …

Generation Of Tanapoxvirus Ankyrin Repeat Gene Deletion Mutant For Evaluating Its Role In Cell Tropism, Yogesh Suryawanshi

Masters Theses

The host range of poxviruses is highly regulated and is limited to certain hosts. A number of genes in different poxviruses have been identified that contribute to cell tropism. The ankyrin (ANK) repeat genes encode for proteins characterized by the repetitive motifs in its amino acid sequence. These genes have been found to play a role in determining the ability of poxviruses to replicate in certain cell types. Tanapoxvirus (TPV) Kenya genome analysis indicated the presence of six ANK repeat genes of which T146R, T147R and T148R are present in the terminal part of the TPV-Kenya genome. To understand the …

Catalytic Engineering Of The Flagellin Protein, Alexandra M. Haase

Masters Theses

Flagellin is the protein monomer that comprises the bacterial flagella for most bacteria, including Salmonella typhimurium. This protein has attracted attention for protein engineering because it is exported out of the cell, polymerized into stable fibers, is produced in large quantities and is relatively simple to purify. Using rational design with computer modeling, potential active sites in the flagellin protein structure were modeled after the human carbonic anhydrase II tetrahedral zinc binding site. In total, three locations were selected as potential active sites and the necessary mutations were successfully introduced. Flagella formation for the flagellin variants was demonstrated through …

Winter Habitat Selection And Genetic Relatedness Of Southern Flying Squirrels In Southwest Michigan, Sheila M. Miara

Masters Theses

Few studies have examined winter habitat selection for southern flying squirrels (Glaucomys volans) in northern latitudes where winter conditions are harsher than southern latitudes and resource availability is particularly limiting. Winter den tree characteristics and use by 19 southern flying squirrels (10 females and 9 males) were investigated from November through February in 2010-2011 and 2011-2012. The mean home range size using 100% minimum convex polygon for 18 individuals was 3.40 ha (±0.46 0.46 SE) and there was no significant difference between genders (P= 0.56) or ages (P=0.50). The mean 95% fixed kernel home range size was 0.15 …

From A Rodent To A Rhetorician: An Ideological Analysis Of George Alexander Kennedy's Comparative Rhetoric, James Begley

Masters Theses

George Alexander Kennedy, a professor of classics at the University of North Carolina at Chapel Hill, has given birth to a new understanding of rhetorical studies: he argues for the evolution of rhetoric from animals to humans. Using Sonja Foss's methodology of "ideological criticism," this thesis examined Kennedy's case as presented in his book, Comparative Rhetoric: an Historical and Cross-Cultural Introduction. This study discovered that the book was heavily influenced by a secular, pro-evolutionary ideology which dually contributed to its selective use of scientific evidences and production of inconsistent arguments. Evaluated on the basis of Biblical principles, this thesis concluded …

Studies On Enzyme Functional Evolution In The Sabath Multigene Family Using Phylogenetic And Biochemical Approaches, Ruiqi Huang

Masters Theses

Gene duplication is believed to be the major source for providing genetic materials for the innovation and diversification of protein functions; natural selection and/or neutral drift then works on these genetic materials to guide their evolutionary directions. Here, I used the salicylic acid/benzoic acid/theobromine (SABATH) multigene family to study how natural selection acted on duplicated genes to prompt functional diversification. Members in this family methylate plant secondary metabolites by transferring the methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group or ring nitrogen of the substrates. In the Apocynaceae and Solanaceae lineage of this family, I documented three putative gene …

Effects Of Ailanthus Altissima Soil Leachates On Nodulation And Expression Of Two Genes That Regulate Nodulation Of Trifolium Pratense, Jesse Michael Lincoln

Masters Theses

Ailanthus altissima (Simaroubaceae) is an invasive tree from China that has spread over much of North America. A number of characteristics contribute to its success, notably tolerance of nutrient-poor soils, rapid growth rates, prolific production of wind dispersed seeds, escape from predation, and the production of allelopathic compounds. That Ailanthus altissima frequently co-occurs with legumes in nutrient poor soils alludes to a possible relationship between Ailanthus altissima and nitrogen fixing legumes. Legumes increase the biospheres supply of bio-available nitrogen through facilitating the fixation of atmospheric nitrogen within structures on roots called nodules. Thus, increased nodulation of legumes caused by Ailanthus …

Demographics And Seasonal Abundance Of Sportfish In Three Thermal Habitats Of Coffeen Lake, Anthony Paul Porreca

Masters Theses

Power generating facilities across Illinois provide electricity to residents of the state, while at the same time offering desirable open water fishing lakes that can be used by anglers during the winter season when other reservoirs are not accessible. Coffeen Lake represents one of the best power cooling fisheries in the state and boasts both excellent largemouth bass and channel catfish populations. The reservoir supplies water to a power station with a generating capacity of 945 megawatts of electricity, with 73% of the surface water affected by heated discharge and a cooling loop covering 4.1 miles. Sampling sport fish species …

The Effect Of An Oral Carbohydrate Rinse On Shorter-Endurance Exercise Program, Morgan Esh

Masters Theses

Research has suggested that orally rinsing with a carbohydrate solution can improve shorter-endurance exercise performance (Carter, Jeukendrup, & Jones, 2004; Chambers, Bridge, & Jones, 2009; Pottier, Bouckaert, Roels, & Derave, 2009; Rollo, Williams, & Nevill, 2011). The purpose of this study was to determine if a carbohydrate mouth rinse improves a 30 minute running distance compared to a non-carbohydrate mouth rinse in females ages 18 to 25 years. Eight female recreational runners performed two experimental rinse-trials the morning after an overnight fast. Participants performed each 30 minute treadmill run at a Borg rating of perceived exertion (RPE) of 14 and …

Population Demographics Of White Crappie In Three Illinois Reservoirs, Candice Marie Miller

Masters Theses

Crappie provide important sport-fisheries throughout the United States. Because of the propensity of this species to display growth stunting, an assessment of crappie population demographics is needed in Midwestern reservoirs. I assessed the population demographics of white crappie, Pomoxis annularis, in three Illinois reservoirs. Samples were collected by three-phase AC boat electrofishing. All crappie were weighed, measured, and the otolith and scale samples were removed for age estimation. Relative density, as measured by catch per unit of effort (CPUE; fish/hour), was different at Lake Mattoon (0.53±0.15), Lake Charleston Side Channel Reservoir (1.17±0.15), and Paradise Lake (1.04±0.18). Size structure of white …

Desiccation Tolerance In Insect Cells, John Marcus Anderson

Masters Theses

In anhydrobiotic animals a reoccurring strategy to survive water stress involves the accumulation of low molecular weight solutes (e.g., trehalose) and highly hydrophilic macromolecules [e.g., late embryogenesis abundant (LEA) proteins]. We utilized two different water-stress models (spin-drying, and hyperosmotic dehydration) to investigate the impact of transgenic expression of a trehalose transporter [BT003466 (TRET)] and of a group 1 LEA protein [ACX81198 variant (LEA1)] on membrane integrity in cell lines from Spodoptera frugiperda (Sf-21) and Drosophila melanogaster (Kc167), respectively. Kc167 cells were challenged with sucrose or trehalose concentrations ranging from 0 to 200 mM supplemented in standard culture medium. Significant inhibition …

Feeding Behavior Of Nestling Eastern Bluebirds (Sialia Sialis) In East-Central Illinois, Amanda Lamb

Masters Theses

I observed feeding of nestlings in Eastern Bluebirds in nest boxes in east-central Illinois. Provisioning rate of males and females were compared and early observation periods (younger nestlings) were compared with late observation periods (older nestlings). I also compared the average food size brought to the nestlings by males and females and during early and late observations. No significant differences were found between average food sizes throughout the study. In addition, food size was not significantly correlated with mean nestling weights within a brood. However, males had significantly fewer confirmed feedings than females for the last observation period. In addition, …

Linking The Colonization Rate Of Vesicular Arbuscular Mycorrhizae (Vam) To Species Dynamics In Succession, Jie Luo

Masters Theses

Vesicular arbuscular mycorrhizae (YAM) are the most common mycorrhizal fungi that form symbiotic associations with plant species. They form hyphae, vesicles and arbuscules which expand root surface area and exchange nutrients within root cells. Greater abundance of YAM can result in more nutrient uptake and disease resistance to the host plant. While mycorrhizae are often important to the growth and reproduction of individual species, their role in regulating plant community dynamics is less clear. Previous research has shown that YAM are tightly related to plant traits such as relative growth rate, foliar N and P concentrations, and responses to soil …

Effects Of Apolipoprotein E, Sex, And Estrogen On The Neuroplasticity Of Olfactory Receptor Neurons In Mice Following Olfactory Bulbectomy, John P. Boyce Iii

Masters Theses

Apolipoprotein E (apoE) is well studied for its role in cholesterol transportation and metabolizing lipoproteins. ApoE is found in the olfactory system especially in the olfactory nerve and glomeruli of the olfactory bulb. Studies from our laboratory indicate that apoE is a required component for timely regeneration and olfactory receptor neuron maturation. The mechanism for which apoE contributes to neuron function and developing late onset Alzheimer's disease is still being studied. A mutant form of apoE (apoE 4) is believed to be a prominent genetic risk factor for this neurodegenerative disease. Over sixty percent of Alzheimer's disease patients in the …

Effect Of Reservoir Discharge And Sanitary Effluent On An Urban Stream, Daniel Hiatt

Masters Theses

Eutrophication of aquatic ecosystems may occur from several sources, primarily from anthropogenic inputs. Excess nutrient deposition can result in eutrophication and community compositional shifts in organisms located in impacted ecosystems. In the Sangamon River located outside Decatur, IL, discharge from the local reservoir, which receives inputs from both a city landscape and agricultural landscape and a wastewater treatment plant (WWTP). These may result in a large influx of nutrients into this system. Increased nutrient levels have the potential to shift benthic periphyton, and macroinvertebrate communities and cause extinction of low tolerance native species. To examine the impacts of inputs for …

Analysis Of A Wound-Induced Gene Family In Glycine Max, Gena Robertson

Masters Theses

Gene families in plants are important in understanding genome evolution indicating when and where genome duplications and segmental duplications occurred as well as subsequent divergence and subfunctionalization. A gene family in Glycine max that encodes a WI12 protein, wound-induced protein, was found to consist of ten genes on five chromosomes. Wound-induced proteins are activated in response to wounding in plants, and the WI12 protein in particular is thought to be involved in cell wall modifications at the wound site. A variety of bioinformatics tools have been used to analyze the expansion of this family in soybean as well as identify …

Physiochemical Characteristics Contributing To The Cytotoxicity Of Transition Metal Oxides, Chi-Heng Wu

Masters Theses

"In the past ten years nanotechnology has not only evolved to play a prominent role in our economy but also increased the concern over potential adverse effects caused by nanomaterials to human health and the environment. Nanotoxicity is to understand the nature and origin of the toxicity imposed by nanomaterials. Studies from our laboratory have shown that nanoparticle induces oxidative stress, perturbs calcium homeostasis, alter gene expression, and produces pro-inflammatory responses. We also identified a trend of toxicity: TiO₂ < Cr₂O₃ < Fe₂O₃ < Mn₂O₃ < NiO < ZnO < CuO. We then asked a question: what are physiochemical factors of transition metal nanoparticles that contributed to this increasing cytotoxicity. In this thesis I investigated the correlation between physicochemical properties and toxicity of the transition metal oxides in the 4th Period of the Periodic Table of Elements. Particle size, BET surface area, point of zero charge, metal dissolution, and degree of surface adsorption of transition metal oxide nanoparticles were measured. There were no increasing trends in both particle size and specific surface area. The point of zero charge showed an increasing trend as TiO₂ < Cr₂O₃ < Fe₂O₃ < NiO < CuO = ZnO < Mn₂O₃. The number of available binding sites of nanoparticle showed an increasing trend as Cr₂O₃ > ZnO > CuO > NiO > Fe₂O₃ > Mn₂O₃ > TiO₂. The degree of adsorption on the surface of nanoparticles showed an increasing trend with atomic …

Social Identity Theory In Campus Recreation: Examining Organizational And Exercise Identification Among Recreation Center Users, Kiel Emberton

Masters Theses

Social identity theory is often used in commercial settings to provide a basis for psychographic market segmentation. This usage has led to the development of organization identification in traditional business settings. Campus recreation is unique in that the primary product of consumption is exercise. In describing exercise behavior, social identity theory has been applied to explain the unique psychological attachments exercisers have to their exercise behavior. This application of social identity theory created the exercise identity construct. The purpose of this study was to examine social identity theory in campus recreation. Specifically, this study investigated the correlation between frequency of …

Whatchu Breathin'?: Sick Building Syndrome And Seasonal Occurrence Of Fungi, Jessica Stapleton

Masters Theses

This study was designed to evaluate the presence of sick building syndrome in a classroom at Unity High School (Mendon, Illinois) and contrast differences in the number of fungal spores per cubic meter of air in different rooms in the building and between seasons. Air and surface samples were taken to analyze the number of fungal spores per cubic meter and to evaluate generic fungal diversity. Factors influencing the number of fungal spores per cubic meter and genera of fungal isolates were year-round temperature control in the classroom and the presence or absence of students. Some of the fungi isolated …

Bioavailability, Bioaccumulation, And Trophic Transfer Of Trace Metals In The Tims Branch-Steed Pond Tributary, Paul Gethyn Edwards

Masters Theses

The Department of Energy's (DOE) Savannah River Site (SRS) is a 777 km² former nuclear weapon material production and current research facility located along the Savannah River in west-central South Carolina. In 1954, direct environmental discharges were released from "M-Area", a fuel and aluminum-clad Uranium (U) nuclear reactor target manufacturing facility through a drainage ditch into the Tims Branch-Steed Pond water system. The wastewater discharges released into Tims Branch contained large volumes of inorganic wastes including depleted and natural U, aluminum (Al), and Nickel (Ni) along with smaller quantities of mercury (Hg), lead (Pb), copper (Cu), chromium (Cr), and …

Egg Recognition And Cowbird Egg Ejection Behavior In The American Robin (Turdus Migratorius), Allison Karlien Lang

Masters Theses

American Robins (Turdus migratorius) typically eject parasitic cowbird eggs from their nests. In order to successfully remove parasitic eggs, robins must first be able to differentiate between their own and foreign eggs and then remove the unwanted egg(s). This study addressed these behaviors. A robin's ability to recognize its own eggs was tested by adding artificial cowbird eggs to robin nests at various parasite-tohost egg ratios which created three treatments: (1) a majority of robin eggs in the nest, (2) an equal number of robin and cowbird eggs, and (3) a majority of cowbird eggs. At 89% of nests (51 …

Comparison Of Silver Carp Population Demographics In An Impounded And A Free Flowing River, Jason G. Stuck

Masters Theses

The silver carp, Hypophthalmichthys molitrix, is an invasive species that has received increased attention due to the proximity of the population in the Illinois River to the economically important Great Lakes and the species ability to dominate the aquatic biomass of an ecosystem. We sought to assess the population demographics of silver carp on two different river ecosystems, the Illinois River and Wabash River. The Illinois River is channelized and dammed for commercial barges, whereas the Wabash River is the longest free flowing river east of the Mississippi River and is still connected to its natural flood plain. Silver carp …