Life Sciences Commons^™

Consumer Evaluation Of Low Sodium Mozzarella Cheese And Development Of A Novel Method For Evaluating Emotions, Lauren Alyse Collinsworth

Master's Theses

Mozzarella cheese is currently the highest consumed cheese in the United States. The popularity of mozzarella cheese is typically attributed to the high consumption rates of pizza cheese and string cheese; both of which are low moisture part skim (LMPS) mozzarella cheese. A single serving of LMPS mozzarella cheese contains approximately 8% of the daily value (DV) for sodium, a mineral which is currently consumed in excess among most Americans. On average, one in three Americans has hypertension. This condition is strongly associated with excessive sodium intake, and it is a leading risk factor for cardiovascular disease and stroke in …

The Comparative Morphology Of The Pectoral Free Rays In Scorpaenoid Fishes (Perciformes: Scorpaenoidea)), Jeremy Peter Harris

Master's Theses

The pectoral fins of many benthic taxa have evolved many unique features used to interact with the substrate. In one such group, the searobins (Triglidae: Scorpaenoidea), three of the pectoral fin rays have become separated from the fin as a whole, allowing them to operate as a separate appendage from the fin as a whole. The anatomical structure of these "free rays" was investigated and several previously unreported muscle and bone variations were found. This method was extended to include members of four other scorpaenoid families which possess free rays, which revealed important differences in the muscle, bone, and nervous …

Analysis Of The Minimal Promoter From The Hatching Enzyme A Gene, Anna Matejczyk

Master's Theses

Hatching, defined as a biochemical or biophysical mechanism that allows the embryo to leave its protective envelope, is found in most animals. In fish, reptiles and amphibians, mostly oviparous animals, this means the emergence of the embryo from an egg. In mammals, viviparous animals, hatching is performed by the blastocyst in order to shed the zona pellucida.

Fish, an oviparous animal, takes advantage of a biochemical mechanism in order to hatch and emerge from their chorion, or egg envelope. The mechanism includes the use of hatching enzymes that are secreted in order to digest the envelope membrane. The genes controlling …

The Effect Of Vitamin D On Human T Regulatory Cell Differentiation, Stephanie Anne Chapman

Master's Theses

Regulatory T cells (Tregs) are required for the induction and maintenance of immune homeostasis. Singh et al. demonstrated that depletion of Tregs in mice results in a loss of self-tolerance that manifests in the development of autoimmune diseases such as Inflammatory Bowel Disease (IBD). In humans, autoimmune diseases such as IBD, Multiple Sclerosis (MS), and Type 1 Diabetes are thought to occur due to a deficiency in the number or function of Tregs. The importance of Tregs in modulating the human immune system is perhaps best exemplified by IPEX (immune dysregulation, polyendocrinopathy, enteropathy, x-linked syndrome), a severe autoimmune disease characterized …

Assessing The Effects Of Titanium Dioxide Nanoparticles On Microbial Communities In Stream Sediment Using Artificial Streams And High Throughput Screening, Alexandra Ozaki

Master's Theses

Nano-titanium dioxide (nano-TiO2) is an engineered nanomaterial used in a wide array of commercial products. The production and use of large amounts of nano-TiO2 is resulting in the unintended release to the environment. Nano-TiO2 is known to be cytotoxic due primarily to its ability to generate reactive oxygen species, and negative impacts on a variety of organisms have been demonstrated, but the effects of nano-TiO2 on complex microbial communities under ecologically relevant conditions have rarely been tested. We conducted a controlled manipulative experiment using recirculating model streams dosed with a one-time amendment of 1mg L-1 nano-TiO2 (specifically P25). Within one …

Behavior And Ecosystem Effects Of The Invasive Asian Clam (Corbicula Fluminea) In Urban Streams, Kayla Turek

Master's Theses

Invasive species can be detrimental to freshwater ecosystems. By completing laboratory and field studies to observe processes and behaviors of the invasive Asian Clam (Corbicula fluminea), I documented pathways whereby this invasive species impacts aquatic ecosystems under conditions typical of urbanized streams I found. The predominant pathways by which clams impacted nitrogen (N) cycling was through excretion thus increasing ammonium (NH4+) flux out of sediment and through bioturbation, which increased nitrate (NO3-) diffusion to the sediment and dinitrogen gas (N2) production (i.e., denitrification). The effect was greater under urban conditions, where C. fluminea population density and water column NO3- were …

Identifying And Characterizing The Degradative Pathway Of The Retroviral Restriction Factor Rhtrim5Α, Rachel Nelson

Master's Theses

Human immunodeficiency virus 1 (HIV-1) is a lentivirus that progresses to acquired immunodeficiency syndrome (AIDS). The protein TRIM5alpha from rhesus macaques (rhTRIM5alpha) restricts HIV-1 by blocking infection after entry of the virion into cells. Treatment of rhTRIM5alpha expressing cells with inhibitors to a cellular degradation pathway, the proteasome, partially relieves restriction but does not inhibit rhTRIM5alpha protein turnover. The role of a second degradation pathway, the autophagy-lysosomal pathway, in TRIM5alpha mediated restriction has not been explored.

In the present study, we demonstrate that rhTRIM5alpha is degraded by chaperone mediated autophagy (CMA). Inhibition of CMA alters rhTRIM5alpha localization and turnover, while …

Evidence For Renewal And Reconstitution Of Marginal Zone Macrophages In Young And Aged Mice, Erika Bahamon

Master's Theses

Aging in humans and mice correlates with decline in immune health, affecting both innate and adaptive immunity. Response against blood-borne bacterial pathogens is compromised because of the deterioration of the marginal zones of the spleen and decrease in frequency of marginal zone macrophages (MZM). This thesis asked if low cell turnover is the cause for the decrease of MZM, using cell proliferation to indicate cell turnover in spleens of mice. First, evidence showed MZM proliferation occurred in spleens of young mice and was decreased in the MZM from aged mice. Second, transfer of young bone marrow into old mice replenished …

Aquatic Macroinvertebrate Response To Invasion And Subsequent Removal Of Typha × Glauca From Great Lakes Coastal Marshes, Kimberly Anne Greene

Master's Theses

Aquatic macroinvertebrates are important in wetland ecosystems; many fish and wildlife species depend upon them for food resources and they aid in nutrient cycling. Wetland macroinvertebrate communities are influenced by plant community composition; as such, this study examined two implications of invasive hybrid cattail, Typha × glauca (Typha), on aquatic macroinvertebrate communities in northern Great Lakes coastal wetlands: 1) how the presence of nearly monotypic stands of Typha alters wetland aquatic macroinvertebrate communities and 2) how manual removal of Typha, via mowing and manual tilling, impacts these invertebrate communities along with wetland plant communities. I found that …

Characterization Of Inducible Regulatory T Cells: An Umbilical Cord Blood Model, Rebecca Anne Krier

Master's Theses

The immune system is a group of structures and processes that protect us from disease. To function properly it must recognize a wide variety of pathogens such as viruses and bacteria. T cells play a crucial role in an immune response; however, an overactive immune response can lead to autoimmune diseases, therefore it is important that the immune system has the ability to negatively regulate an immune response. In the periphery, regulatory T cells (CD4⁺CD25⁺Foxp3⁺) are involved in the maintenance of self-tolerance and immune homeostasis. Mechanisms involved in the induction of iTregs from naïve …

Activity Patterns And Spatial Resource Selection Of The Eastern Garter Snake (Thamnophis Sirtalis Sirtalis), Matthew Gregory Most

Master's Theses

Snake species usually exhibit seasonal variations in activity patterns, home-range size and the use of respective habitat. Using mark-recapture protocols I marked 96 individual Thamnophis sirtalis sirtalis specimens in Lake Forest, IL to determine the independent variables that best explained habitat selection in a population of Eastern Garter Snakes (T. s. sirtalis). Specifically, I focused on the relationship between seasonal movement and home range size of male and female garter snakes. I analyzed the habitat preferences and spatial ecology of Eastern Garter Snakes during the 2011 sampling season and modeled the relationship between preferred or avoided habitat, in conjunction with …

Exosomes: Antiviral Agents In The Human Lung, Jennifer Alexandra Novak

Master's Theses

Hundreds of interferon effectors comprise the immediate host response to virus infection. One family of interferon stimulated genes is the IFITM (Interferon-Induced Transmembrane) proteins. IFITM3, in particular, has been shown to block virus cell entry of Influenza A Virus, SARS Coronavirus, and West Nile Virus, among others. IFITM3 has also been identified as an essential factor for host protection against Influenza A Virus in mice and humans. However, the mechanism by which IFITM3 functions remains unclear.

IFITM3 may function both intracellularly and extracellularly to block virus infection in the human lung. The extracellular milieu contains microscopic vesicles termed exosomes which …

Selective Expansion Of B Cells By Intestinal Microbiota, Karina Ochoa

Master's Theses

In rabbits, antibody diversity and B cell expansion are generated in gut-associated lymphoid tissues (GALT), in an antigen- and T cell-independent mechanism, and require interaction with intestinal microbiota. I investigated, in vitro, the mechanism by which commensals drive GALT reactions. Bacteria were isolated from the GALT of rabbits and identified by 16sRNA sequencing. I found that the commensals can bind to Ig, independently of their specificity. In addition, a ~20kDa bacterial molecule was immunoprecipitated with recombinant Ig molecules. Stimulation of B cells with selected bacterial molecules induced the activation of B cells. Stimulation of B cells through TLR2 induced the …

Antibodies To Inhibit Clostridium Difficile Adhesion To Human Gut Epithelial Cell Line, Wonbeom Paik

Master's Theses

Clostridium difficile infection (CDI) is a major hospital-acquired diarrhea that can cause life-threatening complications such as pseudomembranous colitis. CDI is caused by colonization of host with C. difficile, a Gram positive, anaerobic bacterium known to produce toxins that cause disease. Normally, the gut microbiota protects the host from CDI, but disruption of the microbial composition through antibiotic treatment can leave one vulnerable for CDI. To date, no vaccines for preventing CDI are available.

In this study, the potential of antibodies directed against specific surface molecules of C. difficile to block bacterial adherence to host gut epithelial cells was studied, in …

Forest Regeneration On The Osa Peninsula, Costa Rica, Manette E. Sandor

Master's Theses

Woody species diversity of secondary forest has the potential to converge with that found in old growth forest. This study is the first to examine multiple aspects of species and reproductive trait diversity, and their relationship to each other, across a successional chronosequence. Species richness and species diversity increases with increasing age of forest. Diaspore size and diversity as well as fruit size generally increased with increasing age of forest, but fruit size diversity did not. Abundance of animal-dispersed species increased whereas wind-dispersed species decreased in abundance over succession. Insect-pollinated individuals were most abundant overall. Diaspore diversity, pollination diversity, and …

Detection Of Genomic Inversion From Single End Read, Pankaj Ghimire

Master's Theses

Structural Variations (SVs) are genomic rearrangements that include both copy-number variants,such as insertion,deletions, duplications and balanced variants like inversion and translocations. These SVs are getting more attentions for research and investigation because of their role on human phenotype, genetic diseases and genomic rearrangements. Evolution of Next-generation Sequencing has provided golden opportunities to investigate these variants and make their wider and clear spectrum in human genome. This investigation includes identification of type of SVs and their breakpoints at base pair level. For their effective identification and breakpoint resolution, many techniques are devised mainly based on paired end read. With relatively low …

Effects Of Neonatal Hypoxic Ischemic Brain Injury On Spatial Working Memory, Amanda L. Smith

Master's Theses

Children born prematurely or at very low birth weight (VLBW) have an increased risk for hypoxic ischemic brain injury (HI). HI refers to a lack of adequate blood and oxygen flow in the brain. HI can also occur in the term infant due to birth complications such as prolonged labor, placental dysfunction, or cord prolapse. In both populations (though exact patterns of neuropathology vary) brain damage is likely to occur in the form of decreased hippocampal and cortical volume, and enlargement of the ventricles (Kesler et al., 2004, Nagy et al., 2009). Resulting neuropathology can in turn lead to cognitive …

Tremulous Jaw Movements Induced By The Vmat2 Inhibitor Tetrabenazine, Samantha J. Podurgiel

Master's Theses

Parkinsonism is a movement disorder characterized by several cardinal motor symptoms: resting tremor, akinesia, bradykinesia, rigidity, and postural instability. Parkinsonian resting tremor can be modeled in rodents using the tremulous jaw movement model. Tremulous jaw movements (TJMs) are defined as “rapid vertical deflections of the lower jaw that resemble chewing but are not directed at any particular stimulus.” TJMs occur in a frequency range of 3-7 Hz and are induced by a number of pharmacological manipulations that parallel those seen in human Parkinsonism including dopamine (DA) depleting agents, DA antagonists, and cholinomimetic administration. Additionally, TJMs can be attenuated using antiparkinsonian …

Theta Dynamics: Speed, Acceleration And Contribution To Cognition, Lauren L. Long

Master's Theses

No abstract provided.

An Analysis Of Human Disturbance To Rocky Intertidal Communities Of San Luis Obispo County, Grant Tyler Waltz

Master's Theses

ABSTRACT

An Analysis Of Human Disturbance To Rocky Intertidal Communities Of San Luis Obispo County

Grant Tyler Waltz

The number of coastal areas open to public access in California and San Luis Obispo County is increasing due to the acquisition by California State Parks of land previously owned by private entities. For example, California State Parks acquired property from the Hearst Corporation in 2005, which included 18 miles of coastline. California State Parks is responsible for providing public access in these newly acquired areas and also for maintaining the health of the natural systems found on these properties. Part of …

Effects Of Silvicultural Management On Coast Redwood Forest Composition, Density And Structure In Santa Cruz And San Mateo Counties, Michael J. Papa

Master's Theses

The multiple-objective exploratory study investigates effects of various silvicultural management regimes commonly applied to coast redwood (Sequoia sempervirens [D. Don] Endl.) forests in Santa Cruz and San Mateo Counties, California, USA. A temporary forest inventory was installed in 24 harvest origin stands and 4 natural origin stands throughout the study area (sample area = 1189 acres). Data from the systematic sample of 233 one-quarter acre nested cluster plots (sample intensity = 4.9%) rendered overall forest descriptions in terms of species composition, density, and structure. The common forestry measures of trees per acre (TPA), basal area per acre (BA), and …

Identifying And Characterizing Non-Coding Rnas In The Dinoflagellate Karenia Brevis, Helen Namataka

Master's Theses

When algal cells proliferate and accumulate in marine and fresh water systems, they form algal blo'oms. The majority of these blooms are beneficial, but a significant number are detrimental and are known as harmful algal blooms (HABs). A number of negative effects, including closing of recreational beaches and economic loss, are observed during HABs. Predictably, the longer a bloom persists, the greater its effects on human, environmental and economic health. Karenia brevis, a mixotrophic dinoflagellate, forms HABs, and blooms caused by this organism have been known to remain several months after formation. For these reasons, research has been conducted to …

Fungal Biofilm Colonization And Succession On Artificial Reefs In The North-Central Gulf Of Mexico, Amy Leigh Salamone

Master's Theses

This study represents the first characterization of natural mixed-species fungal biofilm communities on artificial reefs in the marine environment. Previous fungal biofilm studies have failed to observe the naturally-occurring biodiversity in coastal areas, which comprise the most utilized zone of the marine environment. Artificial reefs can increase fishing yields, amplify oyster habitats, contribute to shoreline stabilization, promote good water quality, and deter pollution. Proper management practices of artificial reefs require knowledge of the colonization of these new habitats by marine organisms and their subsequent succession. Fungi, along with bacteria, archaea, protists, algae, and diatoms, quickly form a complex biofilm on …

Mortality Assessment Of Redwood And Mixed Conifer Forest Types In Santa Cruz County Following Wildfire, Steve R. Auten

Master's Theses

On August 12, 2009, the Lockheed Fire ignited the west slope of the Santa Cruz Mountains burning approximately 7,819 acres. Foresters and other land managers were left with challenging decisions on how to evaluate tree mortality. Big Creek Lumber Company, California Polytechnic State University, San Luis Obispo (Cal Poly)’s Swanton Pacific Ranch (SPR), and other resource professionals familiar with this region teamed up to develop a method for evaluating damage and thereby mortality for redwood, California nutmeg, live oak, tanoak, California bay, Pacific madrone, big leaf maple, Douglas-fir, Monterey pine, and knobcone pine. Quantitative damage criteria were used to design …

Post-Fire Mortality And Response In A Redwood/ Douglas-Fir Forest, Santa Cruz Mountains, California, Garren M. Andrews

Master's Theses

We investigated how fire severity impacts the survival and response (sprouting/seeding) of multiple species in the Santa Cruz Mountains of coastal California, including coast redwood (Sequoia sempervirens), Douglas-fir (Pseudotsuga menziesii), tanoak (Lithocarpus densiflorus), and Pacific madrone(Arbutus menziesii). During August 2009 the Lockheed Fire burned nearly 3,160ha of mixed-conifer stands with variable severity. Data from 37 Continuous Forest Inventory (CFI) plots were collected immediately before and for 2 successive years following the 2009 Lockheed Fire.

This research entails three objectives. First, we quantified post-fire mortality of trees that vary in species, size, and …

Low Cost Neurochairs, Frankie Pike

Master's Theses

Electroencephalography (EEG) was formerly confined to clinical and research settings with the necessary hardware costing thousands of dollars. In the last five years a number of companies have produced simple electroencephalograms, priced below $300 and available direct to consumers. These have stirred the imaginations of enthusiasts and brought the prospects of "thought-controlled" devices ever closer to reality. While these new devices were largely targeted at video games and toys, active research on enabling people suffering from debilitating diseases to control wheelchairs was being pursued. A number of neurochairs have come to fruition offering a truly hands-free mobility solution, but whether …

House Finches, Carpodacus Mexicanus: Hormones, Stress, And Song Control Regions, Katherine Olivia Ganster

Master's Theses

Song production in songbirds is controlled by parts of the brain known as the song control regions (SCRs). During spring, gonads increase in size, males sing to attract mates, and SCRs become larger. This neuroplasticity is controlled by the change in day length and increased plasma testosterone (T) levels. Plasma T can be reduced by stress through the production of corticosterone (CORT), through the production of beta-endorphin, or through direct effects on the testes via the nervous system. We determined the T, estradiol, and CORT hormonal profiles of wild House Finches by capturing and sampling blood from the finches every …

Biochemical Evaluation Of Srh Analogs As Potential Luxs Inhibitors, Richa Gupta

Master's Theses

Quorum sensing in bacteria is a process of cell-to-cell communication facilitated by small molecules called autoinducers. Interspecies quorum sensing is facilitated by the autoinducer AI-2. The enzyme LuxS catalyzes the formation of AI-2 from S-ribosyl homocysteine (SRH) in a wide variety of bacterial species. Inhibition of LuxS would therefore inhibit interspecies quorum sensing. The goal of this project is to establish biochemical assays for the evaluation of small molecules as potential LuxS inhibitors. The first assay is a conventional colorimetric assay that utilizes Ellman’s reagent to quantify the homocysteine byproduct of DPD production by LuxS. For this assay purified …

The Relationship Between Liv-1 And E-Cadherin (Cdh1) In Prostate Cancer Cells, Lei Cao

Master's Theses

BACKGROUND

The zinc importer LIV-1 is widely distributed, mainly in hormonally controlled tissues. Attention has focused on its role in breast cancer, especially its regulation by estrogen and epidermal growth factor (EGF) and its link to the Epithelial–Mesenchymal Transition (EMT) marker, E-cadherin (CDH1). EMT is important for tumor progression and metastasis. CDH1 expression is under complex control, including by two transcriptional repressors, Snail and Slug. Contrast to CDH1, elevated MMP-9 expression has been linked to increased metastasis and tumor stage.

METHODS

The role of LIV-1 in prostate cancer cell lines, LNCaP and DU145 was investigated. Cells were treated with or …

Green Tea Extract Protects Against Fibrogenesis Associated With Nonalcoholic Fatty Liver Disease In Diet-Induced Obese Rats, Allyson M. Bower

Master's Theses

No abstract provided.