Life Sciences Commons^™

Atp Defendent Na+ Transport And The Characterization Of Na+ / Nh4+ Exchange In Basolateral Vesicles Of The Blue Crab, Callinectes Sapidus, Gill, Linda Ann Fuhrman

Master's Theses

ATP dependent Na⁺ transport was denonstrated in purified ba.solateral vesicles of the blue crab (Callinectes sapidus) gill. Active Na⁺ uptake was dependent upon the intravesicular presence of either K⁺ or NH; as a counterion. Final membrane preparations consist of inside-out basolateral vesicles, evidenced by intravesicular ouabain (1 rrM) and extravesicular vanadate (1 rnM) ²²Na ⁺ uptake inhibition. Na⁺/NH₄⁺exchange rates were investigated by detennining the NH; concentration in the recovered extravesicular nedium and by using the kinetic capabilities of a DU7 spectrophotareter. These purified basolateral membranes, rich in Na⁺,K^{+ …}

The Effects Of Stress On The Blood Calcium Level In The Male White Rat (Rattus Norvegicus), Howard Perry Cobb

Master's Theses

THE EFFECTS OF STRESS ON THE BLOOD CALCIUM LEVEL IN THE MALE WHITE RAT (Rattus norvegicus) by Howard Perry Cobb I I I was writ ten as part of the requirements for a Master of Science degree in Biology at the University of Richmond (May, 1985). The present experiment was designed to determine whether parathyroid hormone (PTH) can be considered a "stress" hormone. Parathyroidectomized (PX) male rats (160-200 g) were injected with 10, 20, or 30 USP units of PTH per 100 g body weight and subjected to confinement/UHF stress for a 1.5-h period. Serum calcium levels of these PX …

A Macrofaunal Survey Of Three Contrasting Habitats Of The Littoral Zone In Cedar Lake, Illinois, Laura L. Holt

Master's Theses

No abstract provided.

The De Novo Biosynthesis Of Cytokinins By Cell-Free Extracts Of Baker's Yeast, Debra A. Tonetti

Master's Theses

No abstract provided.

Survey Of Intestinal Parasites In Zoo Populations Of Two Central Illinois Zoos And Study Of Current Anti-Parasitic Drugs And Prophylactic Techniques, Verona A. Barr

Master's Theses

No abstract provided.

Regulation Of Nitrate Reductase Activity In Soybeans, Luke Curtis

Master's Theses

No abstract provided.

Factors Affecting Germ Tube Formation In Candida Albicans, Walter A. Tatarowicz

Master's Theses

No abstract provided.

Observations On The Adherence Of Bacteroides Bivius To Female Genital Tract And Oral Epithelial Cells, Susan V. Meade

Master's Theses

No abstract provided.

Effects Of Adrenalectomy On Hemoconcentration And Blood Ph, Urea And Electrolytes In The Mongolian Gerbil (Meriones Unguiculatus), Magally Aellos De Cova

Master's Theses

The effects of adrenalectomy on some blood profiles of the Mongolian gerbil, Meriones unguiculatus, were studied in order to clarify the possible causes of its short survival after adrenal ablation. Measurements of hematocrit, pH, urea and electrolytes were made 18, 24 and 30 hours after surgery. Results show the expected alterations: hemoconcentration, metabolic acidosis, high values of urea and disturbances in electrolytes. While hyponatremia was significant only after 24 hours, important hypochloremia was not present at any time. Hyperkalemia was the most significant alteration observed. It occurred as early as 18 hours after surgery, suggesting that it may be an …

Characteristics Of A Ca++ Atpase Enzyme In The Epidermis Of Molting Blue Crabs, Callinectes Sapidus, Joanne Elizabeth Lapetina

Master's Theses

Epidermal Ca⁺⁺-activated ATPase was investigated in relation to the molt cycle of the blue crab, Callinectes sapidus. Premolt and postmolt tissue samples demonstrated significant elevations in Ca⁺⁺-activated ATPase activity in comparison to intermolt tissue samples. These results support the suggestion that this enzyme may participate in demineralization of the old cuticle, as well as calcification of the new cuticle.

The enzyme is localized in the heavy particulate-nuclear fraction and is distinguished from mitochondrial Ca⁺⁺ ATPase found in other tissues by differences in sensitivity to mersalyl acid. 0 The enzyme is most active at 49 C …

A Survey Of The Living Hermatypic Corals On The West Fore Reef Of Discovery Bay, Jamaica After The Occurence Of Hurricane Allen, James Arthur Crawford

Master's Theses

No abstract provided.

Determination Of An Enzyme Deficiency In The Saccharomyces Cerevisiae Mutant Mod5-1, Lacking Isopentenylated Trnas, Sopiah Suid

Master's Theses

No abstract provided.

Development Of A Dna Probe For The Rapid Detection Of Cytomegalovirus, Nell Lurain

Master's Theses

No abstract provided.

The Effects Of Photoperiod And Melatonin Injections On The Reproductive System Of The Male Mongolian Gerbil (Meriones Unguiculatus), Luis Jose Cova Marchan

Master's Theses

This work was undertaken to study the effects of photoperiod and melatonin on fertility in male Mongolian gerbils (Meriones unguiculatus). Four groups of fifteen animals each, were placed in different photoperiods (ambient, ranging from 14L:10D to 11.3L:12.3D; 24L:10D; 14L:10D; and 1L:23D). After 35 days, three gerbils from each photoperiod condition were sacrificed and the testes, epididymides, seminal vesicles, prostate, kidneys, adrenals, thymus and hypophysis were weighed. The testes and epididymides were placed in Bouin's fixative and used for histological studies. Also, two males from each photoperiod condition were paired individually with a female. The remaining animals of each photoperiod condition …

Non-Visual Feeding Behavior Of The Mottled Sculpin Cottus Bairdi: Ecological And Evolutionary Implications, Denise Maxine Denham Hoekstra

Master's Theses

No abstract provided.

The Role Of Adrenomedullary Hormones In Survival Of The Adrenalectomized Mongolian Gerbil, Meriones Unguiculatus, Carol B. Ussery

Master's Theses

Thee effects of adrenalectomy and catecholamine replacement therapy on survival were studied in Meriones unguiculatus, the Mongolian gerbil. Adrenalectomized gerbils that received epinephrine lived significantly longer than the controls untreated adrenalectomized gerbils) and animals that received either norepinephrine or a combination of epinephrine and norepinephrine. Results also indicated that these epinephrine-treated animals lost more depot fat that either controls or other groups receiving treatment. In spite of the short survival benefit given by epinephrine, the mechanisms governing the critical adrenal dependency in this species remain unknown.

The Role Of The Adrenal Cortex In Fat Metabolism Of Meriones Unguiculatus, The Mongolian Gerbil, Saffia Baggia

Master's Theses

The effects of adrenalectomy and corticoid replacement therapies on survival, weight loss, depot fat and blood free fatty acid were studied in Meriones unguiculatus, the Mongolian gerbil. Adrenalectomized gerbils that received cortisol and aldosterone lived significantly longer and lost significantly more weight than the controls (adrenalectomized gerbils not receiving any treatment) and the sham-operated groups. Cortisol and aldosterone treatment were also effective in mobilizing significantly greater amounts of depot fat into free fatty acid. Cortisol and aldosterone treated groups had significantly less blood free fatty acid than the control and the sham-operated groups.

Some Effects Of Naturally Occurring Amygdalin On Female C3h/Hej Mice, Gareth Eli Shemesh

Master's Theses

No abstract provided.

The Role Of Lh Pulses On In Vitro Follicular Development In The Metestrous Rat Ovary, Shirley Jane Luttmer

Master's Theses

No abstract provided.

An Ecological Succession Model Applied To Environmental Management, Diane Rosenberg

Master's Theses

No abstract provided.

Prevalence Of Baylisascaris Procyonis In Northern Illinois And Larval Migration In The Mongolian Gerbil (Meriones Unguiculatus), Diane Mary Sudduth

Master's Theses

No abstract provided.

An Ultrastructural Study Of The Encapsulation Response Of The American Cockroach, Periplaneta Americana, Candace Ann Ennesser

Master's Theses

No abstract provided.

Reproductive Biology Of The Rat Lungworm Angiostrongylus Cantonensis (Metastrongyloidea: Angiostrongylidae), Grace Spatafora Harris

Master's Theses

Twenty rats (Rattus norvegicus) received infective inocula of 5, 10, 20, and 40 third-stage larvae of Angiostrongylus cantonensis. The effects of varying doses of larvae on prepatent period larval production, worm establishment, worm size, and fertility were studied. The aquatic gastropod Biomphalaria glabratta was the experimental intermediate host.

The duration of the prepatent period varied from 37 to 45 days. The shortest prepatent period was observed in animals that received the highest infective inoculum. The prepatent period showed a significant inverse relationship with increasing inoculum size. Larval production increased logarithmically, followed by a plateau phase. The number of larvae per …

An Electrophoretic Comparison Of Gene Loci In Etheostoma Nigrum And Etheostoma Olmstedi From The James River Drainage, Virginia, Randall Keith Falls

Master's Theses

As many as five different populations of Etheostoma nigrum and Etheostoma olmstedi have been identified in the James River drainage, Virginia. Genetic variation of these populations was analyzed with starch gel electrophoresis and revealed only three distinct populations. Nine loci encoding five enzyme systems and two loci encoding muscle proteins were examined in tissue homogenates. All populations were monomorphic with the exception of the esterase-3 (Est-3), phosphoglucomutase (Pgm-1) and phosphoglucose isomerase (Pgi) loci. Two alleles of Est-3 exhibited activity in the homozygous and heterozygous conditions. Pgm-1 occurred as two alleles with a high degree of heterozygosity (46% for E. nigrum …

The Effects Of Osmotic Stress On Sodium, Potassium, And Chloride Metabolism In The Mongolian Gerbil, Meriones Unguiculatus, Mark William Hagood

Master's Theses

The effects of osmotic stress on sodium, potassium, and chloride metabolism and the physiological mechanisms governing these ions were studied in the Mongolian gerbil, Meriones unguiculatus. Animals were subjected to dietary regimens ranging from sodium-free to high-sodium diets for sixty days. Results indicate the gerbils were not physiologically stressed by the imposed diets. Apparently the animals regulate ion metabolism via intrarenal mechanisms and pituitary antidiuretic hormone while behavior and adrenal cortical activity are insignificant.

Chemical And Ultrastructural Characterization Of Trichophyton Mentagrophytes Arthrospore Walls And Septa, Jordan H. Pollack

Master's Theses

No abstract provided.

Home Range, Primary Home Range, Swimming Speeds And Habitat Preference Of The Largemouth Bass, The Bluegill, The Yellow Perch And The Pumpkinseed As Determined Via Radio Telemetry, Pamela A. Fish

Master's Theses

No abstract provided.

The Spatial Distribution Of Phytoplankton In Westhampton Lake, Richmond, Virginia, James William Newlin

Master's Theses

The hypothesis is that spatial distributions of phytoplankton standing crop and photosynthetic capacity in Westhampton Lake are homogeneous was tested. Distributions were analyzed with respect to horizontal and vertical planes separately and combined. The study was divided into three time period: 1) late summer stratification, 2) early fall mixing, and 3) fall turnover, based on isothermal lines of the lake at the deepest station. Phytoplankton standing crop was measured by spectrophotometric and fluorometric methods. The photosynthetic capacity was measured flurometrically. Combined horizontal and vertical spatial distribution of standing crop was clumped for the entire lake and during each time period. …

The Effects Of Photoperiod And Melatonin Injections On The Reproductive System Of Pinealectomized Male Mice Mus Musculus, Icr Strain, John Earnest Constantine

Master's Theses

One hundred two male mice , Mus musculus, ICR strain, age four weeks, were obtained. Fifty-one mice were pinealectomized and 51 were sham operated. The animals were divided into three photoperiod groups: 1.5L:22.5D, 14L:10D, 24L; 0D. The mice were subcutaneously injected daily with 10 ug melatonin or control solution from post-operative day J to post-operative day 60, On post-operative day 60 representatives of each photo­ period-surgery-injection regimen were paired with females. The remaining animals were killed and several organs were removed and weighed, Melatonin injections were progonadal in pinealectomized mice and inhibitory in sham mice in several instances. Histological examination …

Population Stability Of Three Species Of Zooplankton In Westhampton Lake, Richmond, Virginia, James Christopher Conyers

Master's Theses

Populations of zooplankton, Keratella cochlea's (Rotifera) Bosmina longirostris (Cladocera), and Mesocyclopsedax (Copepoda) were sampled in Westhampton Lake from June 30 to December 22, 1978. Population densities were estimated over time and analyzed for stability relative to the occurrence of rainfall. Two aspects of stability were used for analysis and given operational definitions as follows: Resistance - a population was resistant if its density was not more variable during periods of rain compared with periods of little or no rain; Persistence - a population was persistent if its density during periods of rain was not different from its density in periods …