Life Sciences Commons^™

Utilization Of Geographic Information System For Research, Management, And Education In The Natural Resources Management Department At California Polytechnic State University, San Luis Obispo, David In Yun

Master's Theses

Geographic Information System (GIS) is “an organized collection of computer hardware, software, geographic data, and personnel designed to efficiently capture, store, update, manipulate, analyze, and display all forms of geographically referenced information” (ESRI, 1997a). The Natural Resources Management Department at California Polytechnic State University, San Luis Obispo, California, hereafter NRM, was one of the early users of GIS technology. Although GIS was primarily used as an educational tool, it was also used for cataloging and displaying resource information for management planning and research projects. As the computing technology advanced, GIS became more powerful and easier to use. NRM faculty and …

The Role Of T-Box (Tbx) 2 In Osteoblast Function, Nidhish Francis

Master's Theses

No abstract provided.

The Proteomic Response Of Sea Squirts (Genus Ciona Congeners) To Heat Stress: Evidence For Differential Thermal Sensitivities, Loredana Serafini

Master's Theses

The sea squirts Ciona intestinalis and C. savignyi have disparate distribution patterns, which may result from differences in their thermal tolerance limits. Because C. intestinalis, an almost cosmopolitan species, has a more widespread distribution, it is thought that it is better adapted to endure a wide range of temperatures. In order to compare the heat stress response between these two congeners, we studied global changes in protein expression, using a proteomics approach. To characterize the response to extreme heat stress, animals of both species were exposed to temperatures of 22°C, 25°C, and 28°C for 6 h, and then were …

Investigation Of A Pharmaceutical Compound With Artificial Streams: Effects Of The Antihistamine Cimetidine On Stream Ecosystem Function., Paul David Hoppe

Master's Theses

Pharmaceutical compounds have been widely detected in surface waters but their effects on stream ecosystems are unknown. Cimetidine (Tagamet®), a widely used H2 histamine antagonist has been detected in surface waters. To measure chronic effects of cimetidine on stream invertebrates, I conducted a long-term (83d) artificial-stream experiment. A range of cimetidine concentrations (0.07 μg L-1 to 70.0 μg L-1) were added to streams supporting populations of the amphipod Gammarus fasciatus and beetle Psephenus herricki. P. herricki individual growth rates were reduced in the presence of cimetidine, but G. fasciatus individual growth rates were not different among treatments. G. fasciatus size …

Stream Restoration In A Post-Agricultural System: Indirect Effects On Density And Secondary Production Of Aquatic Insects, Lyle Stanley Dandridge Jr

Master's Theses

Restoration of stream habitats, with the goal of increasing biodiversity through increasing habitat heterogeneity, has been an ongoing trend in recent decades. Current investigations suggest most of those projects fail to significantly influence ecological structure and function when evaluated in light of their affect on species richness. In order to assess the <&ldquo>success of restoration on a prairie stream in northern Illinois traditional metrics such as community composition and density were examined in addition to macroinvertebrate secondary production. Restoration of Nippersink Creek, McHenry County, Illinois was completed in 2000 and this study was conducted 8 years post-restoration. Benthic samples were …

Notch-1 Specifically Activates Erk1/2 In Multiple Breast Cancer Subtypes, Allison Schuyler Rogowski

Master's Theses

Notch-1 is a cell fate regulatory protein and a potent breast oncogene. Notch-1 and its ligand Jagged-1 are over-expressed in human breast cancers that are associated with poor overall survival (Reedijk, Odorcic et al. 2005). Deregulated Notch signaling may contribute to tumorigenesis by increasing proliferation, inhibiting differentiation, and preventing apoptosis (Miele, Golde et al. 2006). The mitogen-activated protein kinase (MAPK) pathway is a critical cell signaling pathway that has been implicated in the development and progression of cancer (Hanahan and Weinberg 2000). Four major MAPK pathways are involved in both cell growth and apoptosis. The regulation of these pathways is …

The Effects Of Kettlebell Training On Aerobic Capacity, Jonathan Asher Falatic

Master's Theses

The purpose of this study was to determine the effects of a kettlebell training program on aerobic capacity. Seventeen female NCAA Division I collegiate soccer players (age 19.7 +1.0 years, height 166.1 +6.4 cm, weight 64.2 +8.2 kg) completed a graded exercise test to determine maximal oxygen consumption (VO₂max). Participants were placed into a kettlebell intervention (KB) group (n = 9) or a circuit weight training control (CWT) group (n = 8). Participants in the KB group completed a kettlebell snatch test to determine individual snatch repetitions. Both groups trained 3 days per week for 4 weeks in …

Detection Of The Sire1 Env-Like Protein, A Potential Retroviral Protein In Soybean, Garen Gaston

Master's Theses

Long terminal repeat (LTR) retroelements are mobile DNAs that are ubiquitous in eukaryotic genomes and include retroviruses, endogenous retroviruses and retrotransposons. Though retrotransposons, which are noninfectious, can make up as much as 80% of plant genomes, no traces of retroviral elements have been confirmed in plants. However, the 2000-copy soybean retrotransposon SIRE1 has an extra open reading frame, that has been described as env-like, because its conceptual translation product is predicted to have features of retroviral envelope proteins, which sponsor infectivity. Demonstrating that this region encodes an envelope protein would extend the range of endogenous retroviruses to plants. Demonstrating also …

The Role Of T-Type Calcium Channels In Vascular Smooth Muscle Cell Cycle Regulation, Abigail Prier

Master's Theses

Results from previous experiments have provided evidence for T-type channel expression in adult pulmonary artery smooth muscle cells (PASMCs) using electrophysiology and qRT-PCR. Studies have also shown that when T-type channels are blocked, either pharmacological or with small hairpin RNA targeted against T-type channel Cav3.1 subunit, mRNA levels are reduced and cell proliferation is inhibited. These results suggest a connection between T-type calcium channels and cell proliferation. In this thesis, Cav3.1 and its possible interplay with cyclin D, an early marker of the cell cycle, was investigated. The time course of cyclin D's translocation to the nucleus to begin the …

Cattail (Typha X Glauca) Invasion In Wetlands Of The Great Lakes Region: Are Impacts Time-Dependent?, Mark Edwin Mitchell

Master's Theses

Some species introduced into new regions have the potential to greatly impact native diversity and ecosystem functioning. The invasive hybrid cattail, Typha X glauca, has established in wetlands across the Great Lakes region decreasing native plant diversity and altering soil and microsite characteristics. We utilized 80 years of historical aerial photographs from the Illinois Beach State Park wetland complex to map the spread and determine the age of T. X glauca stands. Floristic, edaphic, and environmental data were collected from plots across an invasion-age gradient to determine the impacts of T. X glauca and time since invasion on environmental variables. …

Community Structure And Secondary Production Of Aquatic Macroinvertebrates In Coastal Wetland Ponds Of The West Copper River Delta, Alaska, Following Tectonic Uplift, Ryan Glen Van Duzor

Master's Theses

The Great Alaska Earthquake of 1964 (magnitude 9.2) greatly altered the coastal landscape in southcentral Alaska and had particularly dramatic effects on the Copper River Delta (CRD), an ecologically and economically important area within the Chugach National Forest. The earthquake caused tectonic uplift (up to 3.5m) of the CRD coastal tidal marsh and transformed it into a perched freshwater marsh. Copper River Delta ponds, which are crucial habitat to a myriad of migrating songbirds, shorebirds, and waterfowl, are of particular interest to wildlife managers in the CRD and along the Pacific coasts of North, Central and South America. This study …

The River Continuum Redux: Aquatic Insect Diets Reveal The Importance Of Autochthonous Resources In The Salmon River, Idaho, Kathryn Vallis

Master's Theses

Along a river network, changes in the physical characteristics of a stream - changes articulated by the River Continuum Concept (RCC) - have been predicted to influence stream food webs, particularly the resources supporting aquatic macroinvertebrates. Although diets of macroinvertebrates can track resource availability along the longitudinal gradient of streams, insect diets were not assessed in the original RCC study; rather, insects were grouped into functional feeding groups (FFGs). In addition, global climate change is increasing the frequency and spatial extent of wildfire and beetle outbreaks throughout the western United States and could affect riparian vegetation and the amount of …

Role For Histone Deacetylases In Glucocorticoid Receptor Mediated Transpression Of Natural Killer Cell Activity, Kristin Bush

Master's Theses

During periods of psychosocial distress glucocorticoids (GCs) are known to reduce the lytic activity of natural killer cells (NKCA). Glucocorticoid treatment also reduces acetylation of histone residues; however, the glucocorticoid receptor (GR) lacks deacetylase activity. GR is known to interact with histone deacetylases (HDACs) and with corepressors that mediate gene transrepression. In this investigation, GC induced histone deacetylation was demonstrated to be due to GR recruitment of HDAC1 and the corepressor complex SMRT. These data show that reduced acetylation of immune functional genes associated with NKCA is likely due to histone deacetylation by HDAC1 and transrepression of those genes by …

Wee1 Is A Biological Target Of The Mir-17-92 Cluster In Leukemia, Sonia Susan Olikara

Master's Theses

MicroRNAs are noncoding RNAs that bind to the 3' untranslated region of their mRNA targets, which causes downregulation of target gene expression. Previous studies have shown that the miR-17-92 cluster, which encodes six miRNAs, is overexpressed in leukemias arising from chromosomal translocations of the Mixed Lineage Leukemia (MLL) gene. In the present study, prediction algorithms identified WEE1, a kinase that inhibits cell cycle progression, as a possible target of five of the six miRNAs. Through luciferase reporter assays, we found that miR-17, miR-20a, and miR-18a specifically target nucleotides 465 to 487 of the 3' UTR of WEE1, while miR-19a and …

Role Of Type Ii Transmembrane Serine Proteases In Coronavirus Production, Gitanjali Cuckemane Subramanya

Master's Theses

Proteolytic cleavage of Coronavirus spike proteins at the appropriate time and location results in efficient virus entry activation. Type II transmembrane serine proteases (TTSPs), specifically, transmembrane protease serine 2 (TMPRSS2) when expressed on target cells, enhance SARS coronavirus entry by activating spike cleavage. This study investigated the effect of TTSPs expressed in Coronavirus producer cells. Murine Hepatitis Virus strain A59 (MHV A59) viruses produced in the absence of TMPRSS2 required target cell protease activity - either cell surface serine proteases or endosomal acidophilic proteases - for entry. MHV A59 viruses produced in the presence of TMPRSS2 were less infectious overall, …

The Effects Of Nutrient Availability And Season On The Somatotropic Axis In Free-Ranging Alaskan Moose (Alces Alces), Amanda A. Parillo

Master's Theses

No abstract provided.

Investigating Wood Welding Parameters Using A Prototype Welding Machine, Timothy R. Melin

Master's Theses

Understanding how different processing variables influence wood welded bonds is vital if the technique will ever be used to create engineered lumber without using adhesives. A variation of vibration welding, wood welding uses pressure and friction to bond materials together. During welding, heat causes a softening in the wood, a naturally occurring composite material. This softening leads to fiber entanglement and a bond forms upon cooling.

The goal of this research was to investigate several processing aspects of the wood welding procedure. A prototype wood welding machine, designed and fabricated from the ground up, was used to investigate the effects …

Effect Of Sodium Chloride Addition During Diafiltration On The Solubility Of Milk Protein Concentrate, Scott J. Gualco

Master's Theses

There is considerable interest among food manufacturers to incorporate protein into food products in both developed and developing countries. Dairy proteins are excellent choices for many different applications, as they are known to have several nutritional and functional benefits. Membrane filtration techniques are often utilized as the preferred method of fractionation, due to the high throughput and continuous nature of the process. One such product produced from membrane filtration of skim milk is called milk protein concentrate. This product is valued for its high protein content, but it has historically exhibited poor solubility when reconstituted into water, which severely restricts …

Development And Validation Of A Light Weight, Energy Dense, Ready To Eat (Rte) Bar, Jacob Wilhelm-Maria Heick

Master's Theses

DEVELOPMENT AND VALIDATION OF A LIGHT WEIGHT, ENERGY DENSE, READY TO EAT (RTE) BAR

Jacob Wilhelm-Maria Heick

Providing additional calories in the form of an RTE bar to endurance athletes will increase performance and muscle re-synthesis, reduce muscle breakdown, and shorten recovery time. An RTE bar containing a blend of dairy proteins and carbohydrates will create a product with superior functionality, including bioactive and immunity enhancing properties from dairy derived ingredients. The protein will provide benefits in the form of easily digestible calories, essential amino acids and physical satiate.

A formulation was developed and optimized, resulting in a final product …

Reproductive Life History Of Fundulus Jenkensi And Comparative Development Of Five Sympatric Fundulid Species, Eric Thomas Lang

Master's Theses

Fundulus jenkinsi is recognized federally and within the state of Mississippi as a Species of Concern. Little is known about the life history of this coastal killifish, but a detailed reproductive histology study of F. jenkinsi and a diagnostic key of the early life stages of select members of Fundulidae can provide the foundation needed to accurately identify it and quantify reproductive parameters in this rare species in need of conservation. Monthly gonadosomatic index (GSI) of male and female F. jenkinsi were documented, and spawning phases and oocyte stages were examined using reproductive histology. In addition, various stages of …

Explaining The Variability Of Free-Living And Attached Bacterioplankton Abundances In The Bay Of St. Louis, Mississippi, Allison K. Mojzis

Master's Theses

The Bay of St. Louis (BSL) is a shallow and vertically well-mixed estuary on the Gulf Coast of Mississippi and supports the recreational and commercial activities of the surrounding towns of Bay St. Louis, Diamondhead, DeLisle, and Pass Christian. In addition to residential communities, various industrial and commercial sites inhabit the watershed and coastline. The Hollywood casino, DuPont titanium dioxide plant, and multiple sew_age treatment plants, as well as nonpoint sources of pollution, contribute to poor water quality. To evaluate spatial and temporal changes in BSL water quality, freeliving and attached bacterioplankton concentrations were enumerated by epifluorescence microscopy. Nine stations …

Comparisons Of Fish Species Inside And Outside Of Marine Protected Areas Off The South Central Coast Of California, David E. Rasmussen

Master's Theses

COMPARISONS OF FISH SPECIES INSIDE AND OUTSIDE OF MARINE PROTECTED AREAS OFF THE SOUTH CENTRAL COAST OF CALIFORNIA

David E. Rasmussen

From 2004-2008 and 2008-2009 Cal Poly University researchers conducted two separate tag and recapture studies of nearshore fish populations along California’s central coast. Three locations were sampled that have experienced different degrees of fishing pressure. Big Creek MPA has been closed to all fishing from 1993 onwards and is the farthest from port of all locations. Cambria lies closest to port of the three locations and because of this has the highest fishing pressure. Piedras Blancas lies midway between …

Effects Of Changing Attentional Focus Reminder Rates On Learning To Throw Darts, Arya Alami

Master's Theses

The purpose of the study was to examine the effects of an external focus of attention on the learning of dart throwing at three different focus reminder frequencies (every two, every four and every ten trials). Twenty-four male and female subjects were randomly assigned to one of three groups. Subjects threw darts at a circular target 60 times over two days while getting a reminder of their intended external focus at the three different reminder frequencies. Five-day delayed retention and transfer tests were conducted to assess learning, each consisting of five trials. The target was comprised of five concentric circles, …

Dietary Protein Intake, Body Composition And Self-Reported Physical Functioning In U.S. Adults, Kerri Beth Parker

Master's Theses

Sarcopenia is a growing health problem in this country as more Americans are living well into old age. It has been thought that a higher protein intake may be related to greater lean muscle mass as well as greater muscular strength and physical functioning. The purpose of this research was to examine protein intake and its relationship to lean body mass and physical functioning in a nationally representative sample of U.S. adults.

This research used cross-sectional data from the NHANES 2003-2004 to examine the relationship between protein intake, as an average from two 24h recalls, and dual X-ray absorptiometry (DXA) …

Effects Of Wildland Urban Interface Fuel Treatments On Fire Behavior And Ecosystem Services In The Klamath Mountains Of California, Jonathan A. Large

Master's Theses

Greater numbers of people are moving into wildland-urban interface (WUI) areas, increasing the number of people at risk to large wildfires. To mitigate the hazard, emphasis is often placed on fuel treatments used to reduce fuel loads and subsequent fire behavior. This approach overlooks the additional benefits provided by vegetation, including carbon storage and sequestration along with air pollutant removal. This study aimed to calculate and compare differences in representative values by examining a study site in the Klamath Mountains of Northern California. Fire behavior simulations were done under various weather scenarios to illustrate both the impact of weather on …

Burrowing Behavior Of The Fiddler Crab Uca Panacea In Relation To Food Availability, Jennifer A. Mraz

Master's Theses

Much of fiddler crab behavior is regulated by the tides and centers around their burrows. Field and laboratory studies were conducted to assess the effect of food availability on burrowing in the Gulf coast fiddler crab, Vea panacea. In the field, crabs were observed for droving behavior through visual observations; evidence for this behavior was assessed further by determining sediment organic content. Although the organic content did increase significantly as distance increased from the edge of the water, fiddler crabs did not exhibit droving behavior at my study site. Field burrows were cast and measured for depth, diameter and volume …

Effect Of Feeding Zilpaterol Hydrochloride For 20 Days To Calf-Fed Holstein Steers With A 3 Or 10 Day Withdrawal Period Antemortem On Carcass Characteristics And Tenderness, Andrew D. Hosford

Master's Theses

The effect of feeding Zilpaterol hydrochloride (ZH) with a 3 or 10 d withdrawal (WD) period to calf-fed Holstein steers (N=2993) on carcass characteristics and tenderness were evaluated in a feed lot experiment. Cattle were fed 0 or 8.3 mg/kg of ZH for the final 20 d of the feeding period, each treatment level was assigned a WD period of either 3 or 10 d. Treatment groups consisted of Control 3 d WD (C3) and 10 d WD (C10), and ZH fed 3 day WD (Z3) and 10 d WD (Z10). Cattle were slaughtered at a commercial facility, carcasses chilled …

Determining The Fate Of Hybridized Genomes In The Allopolyploid Brassica Napus, Tina Y. Wang

Master's Theses

Polyploidy is widely acknowledged as a widespread mechanism in the evolution and speciation of the majority of flowering plants. Allopolyploid forms through interspecific hybridization and whole genome duplication. While allopolyploids may display increased vigor relative to their progenitors, they can also face challenges to fertility following hybridization. Genetic changes in allopolyploids result from recombination between the hybridized subgenomes, which can influence phenotype and ultimately determine fitness of future generations. To study dynamic changes that follow allopolyploid formation, Brassica napus lineages were derived by hybridizing Brassica oleracea and Brassica rapa. Two lineages of B. napus were analyzed for genetic and phenotypic …

A Comparative Study Of Three Growing Media And Four Plant Groups Under Extensive Green Roof Conditions In San Luis Obispo, Ca, Todd Alan Nelson

Master's Theses

ABSTRACT A Comparative Study of Three Growing Media and Four Plant Groups Under Extensive Green Roof Conditions in San Luis Obispo, California Todd A. Nelson Green roof growing media and plant species are critical factors when deciding on the best materials for a project. Growing media must be chosen in accordance with load bearing capacities of structures and water requirements of the plant material. Plant species must be able to thrive in the climate of the green roof and withstand a green roof’s harsh environment. The objectives of this research were: (1) to determine which of three extensive green roof …

Taxonomy, Biogeography And Pest Status Of Marmara Sp. In Northern Mexico And California Citrus Production, Paul Semet

Master's Theses

A novel lepidopteran sex pheromone lure which was thought to be species-specific to the citrus pest Marmara gulosa (Lepidoptera: Gracillariidae) Guillén (McElfresh et al. 2009) has attracted male moths exhibiting two distinct morphologies. The morphological features examined were the sclerotized parts that make up the male genitalia; which include the valvular lobes, costal lobes, cucullar lobes, and the aedeagus. The sclerotized parts were methodically dissected and mounted on microscope slides. Digital images captured of the slide-mounted specimens proved to be inconsistent with the illustrations provided in the original Marmara gulosa species description (Guillén et al. 2001).

The morphologies of all …