Life Sciences Commons^™

Silage Yield And Quality Of Different Grass Pea (Lathyrus Sativus L.) Genotypes, Murat Azak, Erdem Gülümser

Turkish Journal of Agriculture and Forestry

This study aimed to determine the silage yield and quality of different 12 grass pea (Lathyrus sativus L.) genotypes (Gap Mavisi, İptaş, Karadağ, 1603, 2006, 2401, 4301, 4403, 5001, 6408, 6410, and S3). The trial was established in Bilecik’s ecological conditions in 2022 and 2023. It was carried out according to the randomized block design with four replications, and the plants were harvested at the full flowering stage. The harvested plants were chopped to 2 cm in size and ensiled in vacuum bags and left to ferment at 25 ± 2 °C for 45 days. The silage yield, pH, dry …

Effect Of Different Mulch Materials Applications On Weed Control, Plant Characteristics, And Yield In Garlic (Allium Sativum L.), Ayşe Usanmaz Bozhüyük

Turkish Journal of Agriculture and Forestry

This study was conducted in plots under controlled conditions in the Silvan district of Diyarbakır Province in 2023. The local garlic genotype (Allium sativum L.) from Diyarbakır was used as the plant material. Three different organic mulching materials (sawdust, pine, and straw) were applied at a thickness of 5 cm. The effects of these mulching materials on leaf number, root length, plant height, number of cloves per plant, plant density, bulb diameter, dry and fresh weight of garlic, dry and fresh weight of weeds, weed density, and yield were analysed. A total of 34 weed species from 15 families were …

Appraisal Of Germination Behavior And Activity In Newly Developed Rice Lines, Muhammad Usman Akbar, Muhammad Aqeel, Maryam M. Alomran, Ijaz Haider, Omar Mahmoud Al-Zoubi, Ali Noman

Turkish Journal of Agriculture and Forestry

Rice is a fundamental food source for more than half of the global population. This study evaluated the germination capacity of four rice lines [Sakh (V1), Campena (V2), Kharamana (V3), and Binicol (V5)] and one local variety, KSK-282 (V5). The findings revealed significant differences in the germination attributes among the rice lines. V1 and V3 showed superior germination performance, while V5 consistently exhibited the lowest values. The one-way analysis of variance (ANOVA) showed significant differences (p ≤ 0.05) across multiple parameters, including the coefficient of velocity of germination (CVG), germination index, mean germination rate (MGR), and synchronization index. A significant …

Digital Image Processing In Determining The Degree Of Turnip Yellows Virus Infection In Oilseed Rape, Alina-Ștefania Stanciu, Teodor Rusu, Alin Cristian Teușdea, Olimpia Smaranda Mintaș, Mihai Marcel Cărbunar, Cristian Oneț

Turkish Journal of Agriculture and Forestry

In agricultural crops, leaves play crucial roles in plant physiology, providing information on both the quantity and quality of future production. They also serve as indicators for diagnosing foliar diseases. Detecting plant diseases through image reading and computer vision in a specific format allows for both quantitative and qualitative studies, which is a key step in diagnosing diseases using images of leaves exhibiting symptoms specific to a pathogen. In this study, we present an approach that integrates digital image processing to assess the extent of turnip yellows virus (genus Polerovirus, family Solemoviridae) infection in oilseed rape. This study focuses on …

Seasonal Dynamics Of Biomass Productivity, Combustion Quality, And Nutrient Translocation In Bulbous Canary Grass (Phalaris Aquatica L.) In A Semiarid Mediterranean Environment, Recep İrfan Nazli, Mi̇hrab Polat, Asi̇ye Sena Çavdar, Veyi̇s Tansi

Turkish Journal of Agriculture and Forestry

In order to achieve sustainable biomass production from perennial grasses, seasonal changes in biomass yield, combustion quality, and nutrient translocation need to be monitored in detail. In this study, the effects of six distinct disruptive harvests (November, January, March, May, July, and September) on the dry biomass yield, ash, moisture, and mineral contents, as well as the composition of the cell walls and the translocation of nutrients in bulbous canary grass, were investigated in a semiarid Mediterranean climate. The September harvest significantly reduced aboveground ash and mineral contents, while their contents in belowground biomass exhibited a significantly increasing trend from …

From Ancient Wisdom To Modern Medicine: The Cultural And Therapeutic Significance Of Ficus Carica L. (Moraceae) Extracts In Colorectal Cancer Treatment, Esra Hacimüftüoğlu, Azizeh Shadidizaji, Kağan Tolga Ci̇ni̇sli̇, Songül Karakaya

Turkish Journal of Agriculture and Forestry

Figs, mentioned in the Torah, the Bible, and the Quran, are of historical and cultural significance and have been used for medical treatment, prompting extensive research. Colorectal cancer (CRC), the second leading cause of cancer-related death worldwide, is expected to significantly increase in terms of mortality rates by 2035, with its progression closely linked to inflammatory processes. Cyclooxygenase 2 (COX-2), an enzyme encoded by the Ptgs2 gene, plays a key role in these processes by converting arachidonic acid into prostaglandin E2 (PGE2), a mediator of inflammation. In the present study, the potential of natural antiinflammatory compounds, specifically fumaric acid and …

Erratum To “Coriander (Coriandrum Sativum L.) Diversity Analysis: Insights From Germplasm Resources Leading To Crop Improvement”[Turkish Journal Of Agriculture And Forestry 48 (6) 2024 876-887], Amit Baran Sharangi, Solanki Bal, Anirban Maji, Tarun Kumar Upadhyay, Reem Binsuwaidan, Nawaf Alshammari, Nadiyah M. Alabdallah, Adesh Kolapkar, Mohd Saeed

Turkish Journal of Agriculture and Forestry

We hereby issue this erratum to address a concern regarding the affiliation section in the manuscript titled “Coriander (Coriandrum sativum L.) diversity analysis: insights from germplasm resources leading to crop improvement.” A correction is issued regarding the affiliation detail in the originally published manuscript. The affiliation number for the ninth author, Mohd SAEED, was listed as 7, whereas it should have been 6. To amend this, the affiliation section in the manuscript has been revised as follows:

Link to the original article: https://journals.tubitak.gov.tr/agriculture/vol48/iss6/6/

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.

Early Survival And Growth Of Haloxylon Ammodendron Seedlings Under Irrigation In Arid Southern Tajikistan, Jieun Ahn, Gaeun Kim, Heejae Jo, Jieun Park, Youngjin Lee, Woo-Kyun Lee, Asia Khamzina, Yowhan Son

Turkish Journal of Agriculture and Forestry

Understanding plant drought responses is crucial for effective afforestation in drylands. This study aimed to investigate the effects of irrigation (31.25 mm seedling–1 every 45 days) on the early survival and growth of 3-month-old Haloxylon ammodendron seedlings at an afforestation site established in Tajikistan in 2021. In July 2022 and June 2023, surveys were conducted to compare seedling survival rates, height, root collar diameter (RCD), stem volume per unit area, and height-to-RCD ratio between irrigation (IR) and nonirrigation (NIR) treatments. The IR treatment increased survival rates in 2022 but not in 2023. By 2023, the IR treatment had increased the …

Adapting To Climate Change In Semiarid Regions Via Conservation Measures: Climate-Smart Crop Rotations Of Food Legumes In Cool Seasons, Velibor Spalevic, Mohsen Janmohammadi, Naser Sabaghnia, Shuraik Kader

Turkish Journal of Agriculture and Forestry

The high potential of evapotranspiration, unsuitable soil conditions, irregular distribution of rainfall, and ineffective techniques of soil moisture conservation are among the limitations of semiarid regions of Central and West Asia and North Africa (CWANA), and they have intensified during recent decades with climate change. CWANA’s agroecosystems are fragile and highly vulnerable to the consequences of global warming and aggravation of water scarcity, and need to redefine agroclimatic zoning. Winter cereals are the predominant crops in the region of CWANA. The highest amount of cultivation is allocated to societies with dominant rainfed agriculture, which is often not economically profitable. The …

Remote Sensing Estimation Of Forest Ecosystem Service Values, Yu Zhang, Zhichao Guo, Guoshuang Tian, Minghua Lei

Turkish Journal of Agriculture and Forestry

A precise estimation of the value of forest ecosystem services (FESVs) has become essential due to the growing significance of the influence of global climate change on them. The primary objective of this study was to enhance the precision of forest service value monitoring and evaluation, thereby providing a scientific foundation for the promotion of sustainable forest resource management and ecological civilization construction. The study presented a remote sensing estimation (RSE) technique that combined time series characterization (TSC) and double-logistic regression (DLR) in order to achieve this. Through the integration of TSC analysis and a substantial amount of remote sensing …

Assessment Of Chlorophyll Levels In Navel Oranges Based On Remote Sensing Images Of Unmanned Aerial Vehicles, Hu Li, Shijiang Zhu, Wen Xu, Zhongyong Yang, Xinbo Liu, Weiqi Zhang

Turkish Journal of Agriculture and Forestry

The swift, effective, and noninvasive assessment of citrus chlorophyll levels is important for the timely diagnosis of citrus growth status, and the decision-making of orchard management. In this study, unmanned aerial vehicles (UAVs) with multispectral remote sensing imagery were applied to the mandarin orange, through which five vegetation indices [Medium Resolution Imaging Spectrometer Terrestrial Chlorophyll Index (MTCI), Red-edge Chlorophyll Index (CIre), Nitrogen Reflectance Index (NRI), Red, and Normalized Difference Vegetation Index (NDVI)], which better reflect citrus photosynthetic activity and productivity, were screened. The stepwise linear regression (SLR) method, support vector machine (SVM) regression method, and radial basis function neural network …

A Yolov7-Based Robotic Harvesting System For Agaricus Bisporus Using A Depth Camera, Fengwu Zhu, Weijian Zhang, Zhida Li, Tianshi Gao, Qinglai Zhao

Turkish Journal of Agriculture and Forestry

To achieve accurate detection of button mushrooms (Agaricus bisporus), a harvesting method based on the Robot Operating System (ROS) is proposed. This method combines a convolutional neural network named YOLO (You Only Look Once), a depth camera, and a robotic arm working in coordination. The system integrates advanced image recognition capabilities with the robotic arm to improve harvesting efficiency and accuracy. To enhance the model’s detection accuracy while considering the lightweight requirements for mobile deployment, several modules were incorporated into the You Only Look Once (YOLO)v7 network. Experimental results showed that the improved YOLOv7 network increased the average detection accuracy …

Postharvest Application Of 1-Methylcyclopropene And Modified Atmosphere Packaging Extends Shelf Life And Maintains Eating Quality Of Mango Fruit Cv. Sufaid Chaunsa, Hafiz Muhammad Usman, Sajid Hussain, Raheel Anwar, Ahmad Sattar Khan, Aman Ullah Malik, Muhammad Moaaz Ali, Khurram Ziaf, Arooba Abbas

Turkish Journal of Agriculture and Forestry

The mango (Mangifera indica L.) fruit is globally relished for its sweet taste, unique aromatic profile, and enriched nutritional value. However, its short postharvest life is a serious challenge for export to long-distance markets. 1-Methylcyclopropene (1-MCP) is an ethylene inhibitor known to delay ripening in many fruits, including mango. However, comparative research suggesting an appropriate method to treat mangoes with 1-MCP is limited. In this study, mango fruit cv. Sufaid Chaunsa were fumigated with 0.25 ppm 1-MCP or treated aqueously with 1.2 ppm 1-MCP for 5 min and then packed in modified atmosphere packaging (MAP). The control fruits were not …

Effect Of Eco-Friendly Fertilizers And Edible Coatings On Fatty Acids And Phenolics In Fresh-Cut Melon During Cold Storage, Firat İşlek

Turkish Journal of Agriculture and Forestry

The demand for convenient and ready-to-eat food has increased, including fresh-cut fruit and vegetables. At the same time, people are also becoming more health-conscious and are aware of the positive impact of healthy food choices on their well-being. For this reason, it is important to deliver fruits and vegetables to people’s tables using both environmentally and human-friendly practices, from cultivation to postharvest storage. In the current study, in order to support agricultural sustainability, humic acid, liquid vermi¬compost, and organomineral fertilizers that do not pose a risk to human health or the environment were used in melon. Furthermore, several edible coverings …

Allelic Diversity Of Azerbaijani Bread Wheat (Triticum Aestivum L.) By Ssr Markers, Javi̇d Ojaghi, Sevi̇nj Nuriyeva, Sami̇ra Salayeva, Mahammad Eldarov, Zeynal Akparov, Jamala Eldarova Mursalova

Turkish Journal of Agriculture and Forestry

The objectives of this research entailed studying genetic variation in 50 Azerbaijani wheat accessions from 6 different botanical varieties using simple sequence repeat (SSR) markers. On the basis of the seven SSR primers used in this work, 42 different alleles were observed among the wheat accessions studied with an average of six alleles per locus. Polymorphism information content (PIC) ranged from 0.428 to 0.772, revealing the existence of rich genetic diversity in Azerbaijani wheat accessions. The highest PIC values were calculated with the Xgwm190, Xgwm337, and Xgwm261 SSR primers with a mean value of 0.561. Cluster analysis representing the Nei …

Exploring The Impact Of Planting Density And Environmental Variables On Maize Yield Productivity, Bojan Drašković, Bojan Mitrović, Velimir Mladenov, Ivana Mitrović, Milena Janković, Miroslav Zorić, Nenad Đurić

Turkish Journal of Agriculture and Forestry

Our study investigated the performance of various maize hybrids across diverse environments and planting densities, with the aim of understanding the complex interaction between genotypes, environmental conditions, and planting practices. A set of 13 experimental maize hybrids and two commercial check hybrids was tested over a 3-year period at eight different locations and under two distinct plant densities using a split-plot design. The results revealed significant variations in grain yield (GY) among hybrids across different environments, suggesting a substantial genotype-by-environment (G × E) interaction effect, particularly of the crossover type. This variability was attributed to fluctuations in environmental factors such …

Predicting The Suitable Habitats Of Alosimus Marginicollis (Haag-Rutenberg, 1880) (Coleoptera: Meloidae) And Evaluating Its Potential Distribution In Relation To Geographical And Climatic Barriers, Muhammed Ari̇f Demi̇r, Mahmut Kabalak

Turkish Journal of Zoology

The main objective of this study was to predict the ecological requirements of Alosimus marginicollis (Haag-Rutenberg, 1880), for which limited ecological information is available, and to predict possible suitable habitats under current conditions and different future scenarios. An ensemble model-based approach, combining multiple algorithms (GAM, GLM, MARS, MaxEnt), was used to predict suitable habitats for this species using bioclimatic, topographic and land cover variables. The models indicated that the elevation variable among the topography variables, BIO15 (precipitation seasonality) and BIO4 (temperature seasonality) among the bioclimatic variables, and the cropland variable (cultivated and managed vegetation cover) among the land cover variables …

Morphological, Linear, And Geometric Morphometric Differences (Skull, Mandible, And Pelvis) Among Subspecies Of Talpa Levantis (Eulipotyphla: Talpidae) With Molecular Divergence (Mtdna And Nuclear Dna), Ahmet Yesari̇ Selçuk, Alaetti̇n Kaya, Haluk Kefeli̇oğlu

Turkish Journal of Zoology

In this study, we conducted comprehensive analysis to determine the morphological, linear morphometric, and geometric morphometric differences between three subspecies of Talpa levantis: T. levantis levantis, T. levantis dogramacii, and T. levantis transcaucasica. Molecular analyses including mitochondrial DNA (complete cyt b gene) and nuclear DNA (BRCA-2) were also used. The subspecies of T. levantis showed similarities in the parastyle of the first upper molar and the mesostyle of the second and third upper molars, as well as in the morphology of the pelvis and linear measurements of the ventral skull, mandible, and pelvis. T. levantis dogramacii and T. levantis levantis …

Did Raja Asterias Ever Occur In The Sea Of Marmara Or Were Previous Records Just Cases Of Misidentified Raja Clavata?, Hakan Kabasakal, Uğur Uzer, Fi̇rdes Saadet Karakulak

Turkish Journal of Zoology

The current inventories of elasmobranchs in the Sea of Marmara have some taxonomic problems that need to be solved. One problematic species in the elasmobranch fauna of the region is Raja clavata Linnaeus, 1758, and atypically coloured specimens of this skate species may have been misidentified in the past as congeners or closely related species. The present results show that R. asterias may be a misidentified species of the family Rajidae due to confusion with the atypically coloured R. clavata. As taxonomy is central to the integration of data sources in the context of biodiversity research and management of any …

Integrative Taxonomy Reveals The Existence Of A New Species Of Fat-Tailed Scorpions, Androctonus (Buthidae), In Iran, Hossein Barahoei, Omid Mirshamsi, Masoumeh Amiri, Asma Moeinadini, Ehsan Rakhshani

Turkish Journal of Zoology

Two species of Androctonus have been reported to date from Iran: Androctonus crassicauda (Olivier, 1807) and A. sistanus Barahoei and Mirshamsi, 2022, which is distributed only in the southeast of Iran. Investigating and identifying the distribution range of dangerous species is critically important for scorpion management. A total of 105 specimens of Androctonus were collected from representative locations in Esfahan, Yazd, Kerman, Hormozgan, Hamadan, and Khuzestan provinces. These samples were studied morphologically, morphometrically, molecularly (COI gene sequencing), and through species distribution modeling (SDM) using the MaxEnt model. Four Androctonus species were identified through the morphological and molecular studies: A. crassicauda, …

Corrigendum To “ Phylogenomics Of The Tribe Leonureae Reveals A New Genus: Paraleonurus (Lamiaceae, Lamioideae) , [Turkish Journal Of Botany 48 (Si-1) 2024 427-440] “, Mei-Lin Qian, Ya-Ping Chen, Tuncay Di̇rmenci̇, Yue Zhao, Guo-Xiong Hu, Fei Zhao, Ferhat Celep, Bo Li, Chunlei Xiang

Turkish Journal of Botany

This corrigendum is written to correct an error in our previous publication. The term “ Yi Mu Cao Shu (益母草属; Chinese name).” was incorrectly spelled as “Jia Yi Mu Cao Shu (假益母草属; Chinese name)” in the article. The correct term should be “Yi Mu Cao Shu (益母草属; Chinese name)”. The authors apologize for any inconvenience caused and state that the scientific conclusions are unaffected.

Link to the original article: https://doi.org/10.55730/1300-008X.2825

Complete Genome Characterizations Of Two Novel Mitoviruses Hosted By The Hypogeous, Mycorrhizal Fungi Statesia Pompholyx And Tuber Sp., Ilgaz Akata, Gülce Edi̇ş, Eda Kumru, Emre Keski̇n, Ergi̇n Şahi̇n

Turkish Journal of Botany

The study of viral diversity in uncultivated fungi, including those involved in mycorrhizal relationships, alongside research on viral communities in phytopathogenic and economically important fungi, is crucial for a comprehensive understanding of mycovirus diversity and evolution. Mycorrhizal fungi host various viruses, contributing to our understanding of virus diversity and evolution. Here, we report on the identification and complete genome characterization of two novel mitoviruses that naturally infect the hypogeous, mycorrhizal fungi Statesia pompholyx and Tuber sp. The identified viruses, denominated as “Statesia pompholyx mitovirus 1” (SpMV1) and Tuber mitovirus 3 (TruMV3), have genome lengths of 2549 and 3292 nucleotides and …

Benzothiadiazole Maintains Redox Balance And Promotes Defense Against Sitobion Avenae (F.) In Wheat By Fine-Tuning The Antioxidant System, Secondary Metabolism, And Osmolyte Accumulation., Fayyaz Ahmed, Samina Tanwir, Jam Nazeer Ahmad, Aysha Kiran, Faiz Ahmad Joyia

Turkish Journal of Botany

Aphids are destructive cereal pests causing serious yield losses in the wheat crop. Since wheat is a staple food for more than 75% of the human population, therefore, proper management of insect pests is crucial to enhance its productivity and ensure food security. Aphid herbivory induces highly coordinated defense responses in plants at the cellular and molecular level. This innate plant defense system is activated by a signal transduction pathway mediated by plant hormones like Salicyclic acid (SA). In the present investigation an attempt was made to explore the impacts of Benzothiadiazole (BTH), an analogue of SA on wheat plants …

Erratum To "A New Fossil Wood Species Of Ziziphus From The Middle Miocene Of Türkiye And Its Palaeoenvironmental Evaluation, [Turkish Journal Of Botany 48 (2) 2024 91-104] ", Ünal Akkemi̇k, Özlem Toprak

Turkish Journal of Botany

This erratum addresses an issue with the first author’s address in the original published version of this article. T he author regrets to note the omission of a word in the university name in the original address. To rectify this oversight and ensure the accuracy of the published work, the complete university name is provided below for your reference: Ünal AKKEMİK1,*Özlem TOPRAK2 1Department of Forest Botany, Faculty of Forestry, İstanbul University-Cerrahpaşa, İstanbul, Turkiye 2 Tokat Vocational School, Tokat Gaziosmanpaşa University , Tokat, Turkiye

Link to the original article: https://doi.org/10.55730/1300-008X.2798

Hypericum Chishuiense (Hypericaceae, Sect. Ascyreia), A New St. John's Wort Species From Guizhou, China, Ruizhi Bai, Zheng-Lin He, Ferhat Celep, Lu Wang, Chunlei Xiang

Turkish Journal of Botany

Hypericum chishuiense (Hypericaceae), a new species from Chishui County, Guizhou Province, China, is described and illustrated. Molecular phylogenetic analyses, based on five plastid DNA markers (psbA-trnH, psbC-trnS, rbcL, trnL-trnF, and trnS-trnG), place the new species within H. sect. Ascyreia. Morphologically, the new species is closely related to H. stellatum and H. rotundifolium, but it can be distinguished by several features, including broadly ovate, ovate-elliptic to subcircular leaves (vs. oblong-lanceolate or lanceolate to narrowly ovate leaves of H. stellatum) and broadly elliptic, ovate to triangular-ovate sepals (vs. narrowly lanceolate and rare markedly leaf-like sepals H. stellatum), and it can be distinguished …

Erratum To “Acetylcholine Treatments Enhance Growth, Photosynthesis Efficiency And Stress Resilience In Maize Seedlings Exposed To Arsenic, [Turkish Journal Of Botany 49 (1) 2025 13-24] “, Büşra Arikan Abdulveli̇

Turkish Journal of Botany

This erratum addresses an issue regarding the page headers of the article. We regret to note that an error occurred in the page headers of the originally published version of this article. Although Büşra ARIKAN ABDULVELİ was the sole author, the page headers were mistakenly typeset as “ARIKAN ABDULVELİ et al.”. To rectify this oversight and ensure the accuracy of the published work, the page headers have been corrected as follows: “ARIKAN ABDULVELİ

Link to the original Article: https://doi.org/10.55730/1300-008X.2838

Noccaea Bulutii (Brassicaceae): A New Species From Northeast Anatolia, Bariş Özüdoğru, Hayri̇ Duman

Turkish Journal of Botany

Noccaea bulutii is described as a new species from Artvin, in NE Anatolia. It resembles N. phyrgia, N. aptera, N. kovatsii and N. ochroleuca, but easily distinguished from all these species by its dentate basal leaves and elongated sterile shoots. Phylogenetic analyses using the nuclear-encoded ribosomal internal transcribed spacer (ITS) region placed N. bulutii and closely related species in a large polytomic clade, but none of these species formed a subclade with N. bulutii, demonstrating that these species are clearly distinct from each other. The species is described with diagnostic characters, detailed photographs, and taxonomic comments provided. The morphological similarities …

Effects Of Humic Acid And Salt Stress On Growth, Physiological Parameters And Mineral Substance Uptake In Artemisia Dracunculus L. (Tarragon), Serhat Seven, Şener Akinci

Turkish Journal of Botany

In this study, the physiological changes in Artemisia dracunculus L. plants under salinity stress and the remediative roles of leonardite-derived liquid humic acid (HA) were investigated. Salt stress caused a significant decrease in measured growth parameters (plant height, fresh weight of leaves and stems, dry weight of leaves and stems, number of leaves and leaf area). HA had a positive effect on growth parameters at 75 mM NaCl concentration, except for leaf number, but its effect was limited at 150 mM NaCl concentration. The total amount of protein was found to be lower in the leaves of seedlings grown under …

A New Species From Türkiye:Ferula Erzincanica (Ferulinae, Apiaceae), Mehmet Sağiroğlu, Bariş Bani̇, Hayri̇ Duman

Turkish Journal of Botany

In this study, Ferula erzincanica, a new species from East Anatolia, is described and illustrated. The new species shows significant differences from F. rigidula and F. divaricata, particularly by in the dissection of basal leaves, the length and orientation of the inflorescence branches, the length of the pedicels, the undulation of the dorsal wings of the mericarps, and the number of commissural vittae, as well as in the micromorphology of fruits as analyzed by SEM (Scanning Electron Microscopy). In addition, Maximum Likelihood (ML) and Bayesian Inference (BI) phylogenetic analyses based on the nrDNA ITS gene region were performed to determine …