Life Sciences Commons^™

In Vivo Emergence And Seedling Growth Of Ornamental Pepper Seeds Under Diatomaceous Earth Treatments, Deni̇z Hazar

Turkish Journal of Agriculture and Forestry

Propagation by seeds is very useful in ornamental horticulture, especially for flowering plant production. Seed germination and seedling growth are the most sensitive plant developmental stages and, in nurseries, are under the influence of many biotic and abiotic stress factors. Diatomaceous earth is made from the fossilized remains of tiny aquatic organisms called diatoms, and diatomaceous earth as supplemental Si has been shown to be beneficial for plant growth, flowering, and nutrient content under both well-watered and water-stressed conditions. This study aimed to determine the effects of different methods and doses of Si-containing diatomite on in vivo seed emergence and …

A Comprehensive Review Of Fermentation Technologies In Food Processing: From Traditional To Cutting Edge, Pragati Singh, Niharika Arya, Shalini Sharma, Ankita Sharma, Prasad Rasane, Supriya Singh Gaur, Sawinder Kaur, Mahendra Gunjal, Hasan Kiliçgün, Jyoti Singh

Turkish Journal of Agriculture and Forestry

Fermentation technologies play crucial roles in food processing, offering various advantages, including enhanced flavor profiles, extended shelf life, and improved nutritional qualities. This paper provides a comprehensive overview on the major types of fermentation as well as effects of various factors such as substrate composition, temperature, pH, oxygen availability, and microbial strain selection on the process. Despite its widespread application, traditional fermentation processes face limitations related to yield optimization, contamination risks, and process consistency. Recent advancements have significantly enhanced fermentation efficiency, product quality, and scalability, ultimately expanding the applications beyond food and beverage production into pharmaceuticals, nutraceuticals, biofuels, and waste …

Assessment Of Phenolic Composition, Sugars, And Antioxidant Capacity In Turkish Fig (Ficus Carica L.) Cultivars, Ghassan Zahid, Haşi̇m Kelebek, Ayzi̇n Küden, Flavien Shimira, Yildiz Aka Kaçar

Turkish Journal of Agriculture and Forestry

Fig fruits (Ficus carica L.) have significant health benefits. Türkiye is the leading fig producer in the world. Despite their diversity and rich medicinal properties, the biochemical fingerprints and nutritional quality of local figs are poorly understood. This study aimed to analyze the peels and pulps of eight Turkish figs with varying colors for their total phenolic composition, sugars, and antioxidant capacity. Thirty-one phenolic compounds (PCs) were detected using HPLC-MS/MS. The dark-purple 01-1N-58 genotype had the highest amount of colored PCs in fruit peels and pulps (151.25 and 31.57 mg/100 g dw, respectively). Likewise, the maximum quantity of colorless PCs …

Antiinflammatory Mechanisms Of Barley-Based Functional Foods Beyond Diabetes Management, Hafiz Ghulam Muhu Din Ahmed, Adnan Noor Shah, Muhammad Ahtasham Mushtaq, Xiaomeng Yang, Jiazhen Yang, Xia Li, Li’E Yang, Xiaoying Pu, Yawen Zeng

Turkish Journal of Agriculture and Forestry

This review article investigated the antiinflammatory properties of barley, emphasizing its benefits beyond diabetes management. Barley, historically valued for its nutritional and medicinal properties, is rich in bioactive compounds such as beta-glucans, phenolic acids, and flavonoids. These compounds are known for their significant antioxidant and antiinflammatory activities. This study highlights the multifaceted mechanisms through which barley reduces systemic inflammation, including the enhancement of gut microbiota composition, the production of beneficial short-chain fatty acids, and the modulation of immune responses. Beta-glucans in barley lower cholesterol, and regulate glucose levels, while phenolic acids and flavonoids inhibit proinflammatory enzymes and cytokines, thereby reducing …

Effects Of Commiphora Myrrha Resin Extract/Nanosilver On Immune Cells, Pathogen, And Breast/Colon Cancer Cell Line Growth, Mohammad Y. Alshahrani, Essam H. Ibrahim, Abeer S. Alahmari, Mona Kilany, Ramadan Taha, Haitham I. El-Mekkawy, Amr Ahmed El-Arabey, Ahmed Ezzat Ahmed

Turkish Journal of Agriculture and Forestry

Commiphora myrrha grows in various places of the world and is used for many purposes. The objectives of this study were to examine the biological influences of C. myrrha resin extract (MRE) alone or with its biogenic nanosilver on microbial, immune, and cancer cell growth. C. myrrha resin was boiled to prepare the MRE. Functional groups in the MRE were explored using Fourier-transform infrared spectroscopy (FTIR). MRE was used to synthesize nanosilver that was characterized using an ultraviolet-visible (UV-Vis)-spectrophotometer and X-ray diffraction. In vivo experiments included the untreated group (G1) and MRE-orally treated group (G2). Cancer cell lines, HCT-116 (colon) …

Nitrate And Nitrite Levels In Cattle Feed, Drinking Water, Rumen Content, And Blood Methemoglobin Levels In Burdur Province Of Türkiye, Reyda Kiyici, Hasan Altan Akkan, Mehmet Karaca, Esra Zeybek, Bayram Kudret Karaayvaz, Fi̇rdevs Mor Savaş, Yahya Öztürk, Asim Kart, Ramazan Yildiz, Murat Bayezi̇t

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the risk factors associated with nitrate/nitrite poisoning in cattle in the Burdur region. Feed and water samples were collected from 10 different farms randomly selected from 9 districts. A total of 33 feed samples, 13 water samples, 21 rumen contents, and 20 blood samples were collected for analysis. Nitrate and nitrite levels in feed, drinking water, and rumen fluid, as well as methemoglobin levels in blood samples, were determined using a spectrophotometric method. The average nitrate and nitrite levels in feed samples were 3.06 mg/kg and 3.54 mg/kg, respectively. The mean nitrate and nitrite …

Discrimination Of Sprat Species (Clupeonella Spp.) In The Caspian Sea And The Sea Of Marmara Using Otolith Shape Analysis, Mohammad Reza Shariati, Mohammad Sadegh Alavi-Yeganeh, Mehdi Ghanbarifardi, Gülşah Saç, Müfi̇t Özuluğ, Erdoğan Çi̇çek

Turkish Journal of Zoology

This study evaluates the discriminating power of sagittal otolith shape among three species of the genus Clupeonella endemic to the Caspian Sea (Clupeonella grimmi, Clupeonella caspia, and Clupeonella engrauliformis) and one species from the Sea of Marmara (Clupeonella Cultriventris). Identification of these species is challenging due to their morphological similarities, and the taxonomic validity of C. caspia and C. cultriventris remains ambiguous because of their resemblance. Scanning electron microscope (SEM) images were utilized to examine the morphological characteristics of otoliths. Stereomicroscope images were used to calculate six shape indices: rectangularity, roundness, ellipticity, circularity, aspect ratio, and form factor. The outline …

Assessment Of Pyriproxyfen 0.5% (Juvenile Hormone Analog) Against Mosquito (Diptera: Culicidae) Larvae: A Multiscale Study From Laboratory To Field, Neetu Kachhwaha, Vinod Kumari, Rajbala Verma, Nirmala Sharma, Asif Khan, Mohit Singh, Sanyam Nimoria, Priyadarshini Sharma

Turkish Journal of Zoology

Vector control strategies increasingly focus on nonconventional chemicals with high insecticidal efficacy, persistence, and low toxicity to nontarget organisms. The present multiphase study, conducted in laboratory, semifield, and field conditions in Jaipur, Rajasthan, India, evaluated the efficacy of pyriproxyfen (0.5%) (juvenile hormone analog) in controlling mosquito emergence. Laboratory bioassays demonstrated significant susceptibility of Culex tritaeniorhynchus Giles, 1901 (Diptera: Culicidae) larvae, with the first and second instars exhibiting 100% mortality at 1 ppm and third and fourth instars showing emergence inhibition (IE) of 86.11% and 65.28%, respectively. Probit analysis determined lower LC₅₀, LC₉₀, and LC₉₉.₉ values for the first instar larvae …

Distribution Pattern And Abundance Dynamics Of Asian Openbill (Anastomus Oscitans) In Rice Field Throughout Peninsular Malaysia, Badrulhadza Amzah, Rosli Ramli, Noorhidayah Mamat

Turkish Journal of Zoology

The Asian Openbill (Anastomus oscitans) plays a crucial role in rice field ecosystems by preying on invasive apple snails (Pomacea spp.), potentially reducing the reliance on chemical molluscicides. This study investigated the distribution and abundance dynamics of the stork across major rice granaries in Peninsular Malaysia. Surveys were conducted at 111 sampling points within 30 localities across 11 states during five consecutive rice seasons (2022–2024), with bird counts obtained through binoculars and digital photography. Results indicate that storks are highly concentrated on the northern and central west coasts, where rice fields provide abundant foraging opportunities. In the northern regions (Perlis, …

A Singular Case Of Early-Stage Long-Distance Dispersal Of The Smooth Newt, Lissotriton Vulgaris (Linnaeus, 1758) (Amphibia, Caudata), Simone Costa, Francesco Paolo Faraone, Mario Lo Valvo

Turkish Journal of Zoology

To date, the global introduction of alien amphibians and reptiles has likely been facilitated by the expanding plant nursery trade. Here, we report in detail a singular case of unintentional introduction of Lissotriton vulgaris in Sicily. A newt in the final stages of its larval development was found in a private garden pond in north-western Sicily, where aquatic plants from a nursery in Veneto (northeastern Italy) had been introduced two months earlier. The analysis of the mithochondrial markers ND2 and ND4 revealed a shared haplotype between the Sicilian newt and a sample from Treviso, the province of the putative source. …

Molecular Phylogeny And Population Structure Of Eurygaster Integriceps (Hemiptera: Scutelleridae) In The Southeastern Anatolia Region Of Türkiye, Mehmet Sali̇h Özgökçe, Merve Doğaç, Hi̇lmi̇ Kara

Turkish Journal of Zoology

The aim of this study was to determine the identity and distribution of the sunn pest Eurygaster spp. and to quantify the population density in wheat fields across Southeastern Anatolia, Türkiye. A total of 309 samples were collected from 76 fields in 54 districts across all provinces within the study region, where wheat production is intensive. A combination of morphological and molecular techniques was employed to identify the collected specimens. Morphological identification was based on male genitalia, and PCR amplification of the COI mitochondrial gene region was used for DNA barcoding. Both morphological and molecular analyses confirmed that all collected …

Protein Composition Of The Foam Nest Of Rhacophorus Reinwardtii (Schlegel, 1840) (Anura: Rhacophoridae), With Notes On Breeding Sites Characteristics And Biomedical Applications, Muhammad Asyraf Rijalullah, Nia Kurniawan, Nia Kurnianingsih

Turkish Journal of Zoology

Rhacophorus reinwardtii is endemic to Java Island and is currently experiencing a population decline. Therefore, the sustainability of its offspring is important to prevent species extinction. Foam nest as a biosurfactant structure plays a crucial role as egg protection through major protein components function. However, studies on the functional proteins of rhacophoridae foam nests are limited. Thus, this study aims to uncover the protein-specific components and functions of the R. reinwardtii foam nest. Additionally, the characteristics of breeding sites, including abiotic factors, were also assessed. Total protein concentration of the R. reinwardtii foam nest was determined to be 747.7 μg/mL …

Molecular Crosstalk Of Transcription Factors And Hormones In Drought Tolerance Genes In Plants, Cengi̇z Kaya

Turkish Journal of Botany

Water deficit is a critical challenge to global agricultural productivity and food security, which is exacerbated by climate change and increasing water scarcity. To withstand drought stress, plants employ a variety of molecular strategies driven by complex regulatory networks. This review examines the central role of transcription factors (TFs) as master regulators that activate drought responsive genes, which in turn mediate key adaptive processes such as osmoregulation, antioxidative defense, and membrane stability. A distinctive aspect of this review is its focus on the dynamic interplay between TFs and hormonal signaling pathways, including abscisic acid, ethylene, salicylic acid, and jasmonic acid. …

Natural Microbial Consortium Construction Strategy From Rhizospheric Agricultural Soils To Improve Wheat (Triticum Durum Desf.) Seedling Growth, Ni̇da Arslan, Mi̇ne Gül Şeker, Özlem Akkaya, Yelda Özden Çi̇ftçi̇

Turkish Journal of Botany

Microbial consortia of compatible microorganisms play a key role in enhancing agricultural sustainability by optimizing nutrient uptake by promoting plant growth more effectively. This study aimed to identify local isolates with the most effective plant growth-promoting (PGP) properties and to form a consortium of isolates showing the optimal synergistic effect between plant and bacterial components to improve the growth of wheat plants. For this purpose, 48 isolates identified from 145 isolates were screened for their PGP activities. According to the results, 16S rRNA gene sequencing was performed to identify the six isolates exhibiting the most promising activity. Different consortium groups …

Taxonomic Revision Of Korean Salix Section Subviminales Based On Comparative Morphology And Complete Plastome Analysis, Young Moon Kim, Shukherdorj Baasanmunkh, Nudkhuu Nyamgerel, Won Seok Lee, Dong Chan Son, In Kyu Park, Bom Bi Jin, Hyo Sig Won, Hyeok Jae Choi

Turkish Journal of Botany

Salix section Subviminales, including only three species, is endemic to East Asia. In this study, we revised three species, S. gracilistyla, S. graciliglans, and S. blinii in Korea based on their plastomes and extensive morphological analyses to provide a comprehensive understanding of their taxonomic relationships. Based on our findings, we identified the morphological characteristics of S. blinii endemic to Korea, including its small size and distinct leaf shapes, which are well distinguished from both S. gracilistyla and S. graciliglans. Most of the morphological characteristics of S. gracilistyla and S. graciliglans overlapped, particularly leaf shape, floral structure, and growth habits. Next, …

A New Species From Türkiye: Erigeron Akaydinii (Asteraceae), Batikan Günal, Gürkan Semi̇z, Sultan Akyol

Turkish Journal of Botany

Erigeron akaydinii Günal & Semiz sp. nov., belonging to the Trimorpha Cass. (DC) section (Asteraceae), is described as a new species from Uludağ Mountain (Bursa, Türkiye). This study examines the diagnostic traits of E. akaydinii and related species, including morphological, palynological, and molecular analyses. Notes on the ecology, distribution, and conservation status of the new species are also provided, along with a diagnostic key for relevant taxa.

Ecological Assessment Of Lentic Ecosystems In Ceyhan River Basin Using Phytoplankton Indices, Abuzer Çelekli̇, Emi̇ne Yonca Gümüş, Tolga Çeti̇n

Turkish Journal of Botany

Increasing exploitation of water resources has led to declining water quality in aquatic environments. Freshwater ecosystems face various environmental pressures, such as the impacts of climate change and intensifying land-use practices. This study aimed to assess the ecological preferences of phytoplankton communities and determine the ecological status (ES) of several reservoirs and lakes in Ceyhan River Basin across different seasons. A canonical correspondence analysis was conducted to understand the associations between phytoplankton assemblages and stressors in freshwater ecosystems, revealing a strong correlation of 97%. The factors that most influenced the distribution of phytoplankton species were electrical conductivity (EC), temperature, total …

Genome-Wide Association Of Gene Regions Controlling Selenium And Magnesium Accumulation In Walnuts (Juglans Regia L.), İrem Büyükalgan, Bülent Yağmur, Erdal Orman, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş

Turkish Journal of Botany

Walnut (Juglans regia L.) is one of the most economically important species. It is of great importance to the food industry due to its nutritional value, and high content of many beneficial components such as macro and microelements. In the current study, the aim was to determine markers of single nucleotide polymorphism (SNP) associated with the gene(s) controlling the accumulation of Selenium (Se) and Magnesium (Mg) in walnuts. Se and Mg concentrations were measured in 154 genotypes of Juglans regia in 2021 and 2022. A total of 16,473 SNP markers were used for association mapping to analyze the marker-trait associations. …

A New Coralloid Arecoid Palm Root Mat From The Deccan Intertrappean Beds Of India And Its Significance, Sanchita Kumar, Robert Spicer, Mahasin Ali Khan

Turkish Journal of Botany

A new fossil-species of coralloid palm root mat under the organ genus Rhizopalmoxylon is reported from the latest Maastrichtian (Late Cretaceous) to earliest Danian (Early Palaeocene) sediments (c. 66–65Ma) of the Deccan Intertrappean Beds of Madhya Pradesh, Central India. The significant anatomical attributes, specifically an exodermis comprising 2–4 layered parenchymatous cells; a 5–8 layered homogeneous outer cortex, a highly lacunate inner cortex central diffuse layer of aerenchyma with well-developed radially elongated cells arranged in 2–4 rows, and a stele consisting of radial and exarch vascular bundles with proto and metaxylem strands reveal its close resemblance to root mats of extant …

Complete Genome Analyses Of A Novel Narlivirus Identified In The Ectomycorrhizal Fungus Amanita Vidua, Ilgaz Akata, Eda Kumru, Gülce Edi̇ş, Ertuğrul Kaya, Ergi̇n Şahi̇n

Turkish Journal of Botany

The widespread utilisation of deep sequencing has dramatically enhanced and revolutionised our understanding of virus diversity, particularly in complex environments such as soil and biological samples. This research explored mycovirus sequence analyses in the ectomycorrhizal fungus Amanita vidua, utilising deep sequencing alongside total double-stranded RNA (dsRNA) extractions. Our investigation unveiled a unique member of the proposed family, Narliviridae, designated as Amanita vidua narlivirus 1 (AvNV1), in an A. vidua specimen. AvNV1, classified under Amabiliviricetes, possesses a distinct genome structure and sequence attributes. T horough analyses indicated that AvNV1 exhibits sequence similarities with other mycoviruses from the proposed family Narliviridae and …

Unveiling The Physical Interaction Between Gsnor1 And Trx-H Isoforms, Anika Tabassum, Gary J. Loake

Turkish Journal of Botany

This study aims to investigate any possible interaction between two highly conserved regulators of cellular SNO levels: S-nitrosoglutathione reductase (GSNOR1) and thioredoxin (TRX). These two enzymes control global S-nitrosylation levels and thus impact the cellular redox state during immune responses. A mutant line (Δtrx1trx2sfa1) of Saccharomyces cerevisiae MaV203 strain was generated to explore this possibility rather than using a classical strain. We identified and characterized the physical interaction between GSNOR1 and Arabidopsis cytosolic h isoforms in a yeast two-hybrid screen. Out of the seven TRX isoforms, AtGSNOR1 displayed a high interaction with AtTRXh3, AtTRXh4, and AtTRXh5 (AtTRXh5>AtTRXh4 & AtTRXh3). …

Progression Of Electrophysiological Impairments In Diabetic Cardiomyopathy And Intervention Using Enicostemma Axillare, Victor Arokia Doss, Dharaniyambigai Kuberapandian

Turkish Journal of Biology

Earlier studies widely reported advanced ventricular impairments only at the later stages of diabetic cardiomyopathy (DCM). The present study aimed to understand the duration-based early to late stages of cardiac electrophysiological impairments using electrocardiography (ECG) and intervention using Enicostema axillare subsp. littorale. The experimental rats were streptozotocin (SZ)-induced diabetic rats that were administered E. axillare formulations as follows: (1) normal; (2) SZ - 40 mg/kg, single, i.p.); (3) SZ + insulin (2 U/day) + losartan (10 mg/kg); (4) SZ + insulin + losartan + E. axillare decoction (2 mL/day); (5) SZ + E. axillare decoction; (6) SZ + E. axillare …

A Tetracycline-Inducible Split Turboid System For Specific Biotinylation And Identification Of Nuclear Proteins From Hek293t Cells, Mehmet Sarihan, Fatma Zehra Özen, Murat Kasap, Gürler Akpinar

Turkish Journal of Biology

Background/aim: To overcome the limitations of conventional organelle isolation methods including low purity, low yield, sample degradation, scalability and the need for multiple centrifugation steps, an improved nuclear protein enrichment approach was developed using the modified Split TurboID biotin ligase enzyme.

Materials and methods: A construct was created in which the N-terminal domain of TurboID, fused to the FK506-binding protein (FKBP) was targeted to the nucleus. This construct was incorporated into a tetracycline-inducible gene expression vector. Similarly, the C-terminal domain of TurboID was fused to the rapamycin-binding domain of mTOR (FRB) and directed to the nucleus. This construct …

Apigenin Attenuates Ischemia–Reperfusion-Induced Pulmonary Ferroptosis And Fibrosis By Activating The Nrf2/Ho-1/Gpx4 Axis In Mice, Liang Zhang, Haojie Li, Shichen Xu, Hao Wen, Chaoxiao Yu

Turkish Journal of Biology

Background/aim: Acute lung injury (ALI) is a major cause of morbidity and mortality after lung ischemia-reperfusion injury (LIRI). In recent years, pulmonary ferroptosis and its associated fibrosis have been recognized as important causes of LIRI. The purpose of this study is to investigate apigenin (APG) as a potential therapeutic target for treating LIRI-induced pulmonary ferroptosis and fibrosis.

Materials and methods: A rat model of LIRI was established and the rats were randomly divided into three groups, a sham group, a LIRI group, and an APG group. The pathological changes of the lung tissue were evaluated using hematoxylin-eosin staining and …

Prooxidant Effect Of Uric Acid On Human Leukocytic Dna: An In Vitro And Ex Vivo Study, Yim Tong Savio Szeto, Vincy Sze Wing Li, Yuen Lam Pon

Turkish Journal of Biology

Background/aim: Uric acid is a major contributor to the total antioxidant capacity of human plasma. However, this endogenous substance’s antioxidant and prooxidant properties have not yet been reported.

Conclusion: In this study, the comet assay was employed in vitro to determine the effect of uric acid on DNA damage in human lymphocytes and leukocytic DNA damage in hyperuricemia patients with and without renal failure.

Results: DNA damage in lymphocytes occurred at uric acid concentrations of ≥600 μM. Adding catalase to the uric acid solution diminished the damaging effect, indicating that hydrogen peroxide mediated the prooxidant activity. Moreover, …

Intrinsic Apoptotic Effect Of Anatolian Honeybee (Apis Mellifera Anatoliaca) Venom Promoted With Mesoporous Silica Nanocarriers, Batuhan Orman, Ayli̇n Koç, Di̇dem Şen Karaman, Ayşe Nalbantsoy

Turkish Journal of Biology

Background/aim: The use of bee products or treatment with bees, as a complement to conventional medicine is attracting considerable attention in cancer research. Although discoveries related to the potential anticancer effects of bee venom are increasing, the unstable nature of venom biomolecules remains a limiting factor for their usage. In this study, we employed mesoporous silica nanocarriers (MSNs) to provide precise dosing and prevent carriers from biomolecule degradation thanks to the outstanding loading capacity provided by the pores, excellent chemical and biological robustness, and ability to improve bioavailability.

Materials and methods: MSNs were synthesized and physicochemical characterizations were …

Resveratrol Modulates Triosephosphate Isomerase And Mineralization In Osteosarcoma Cells: Potential Target For Novel Therapeutic Strategies, Gonca Tuna, Si̇bel Çinar Asa, Eli̇f Ertürk, Yaren Yildiz, Ferda Ari

Turkish Journal of Biology

Background/aim: Osteosarcoma, a primary malignant bone tumor, is challenging to treat due to its aggressive nature and limited therapies. Resveratrol (RES), a natural polyphenol, has potential anticancer properties. Hence, we investigated RES’s impact on osteosarcoma cells, focusing on triosephosphate isomerase (TPI) and related mechanisms.

Materials and methods: RES was applied to osteosarcoma (SaOS-2) and healthy fetal osteoblast (hFOB 1.19) cells for 48 h, and cell viability was measured by SRB assay. The mode of cell death was examined using Hoechst 33342/annexin V/propidium iodide. TPI and methylglyoxal (MG) enzyme levels were determined by ELISA. The effect of RES on …

N-Cadherin Mimetic Hydrogels Drive Superior Regenerative And Paracrine Responses In 3d Cultures Of Adipose-Derived Mesenchymal Stem Cells, Demet Kaçaroğlu, Alper Murat Ulaşli, Aybüke Di̇denur Sak, Seher Yaylaci

Turkish Journal of Biology

Background: Cadherin-based biomaterials play a pivotal role in influencing the fate of mesenchymal stem cells (MSC). Enhancing the adhesion of adipose tissue-derived MSCs has been shown to augment their paracrine effects while N-cadherin biomaterials have been suggested to regulate the paracrine effects of MSCs via specific growth factors although the precise mechanisms underlying this regulation remain insufficiently understood. This study aims to compare the effects of a 3D N-cadherin mimetic environment on cell viability, apoptosis, extracellular matrix regulation, and growth factor expression with those observed in traditional 2D and 3D spheroid cultures. Additionally, the study seeks to evaluate the …

Knockdown Of Plexin C1 Induces Epithelial-To-Mesenchymal Transition And Confers Resistance To Multikinase Inhibitors In Hepatocellular Carcinoma Cells, Gamze Güngör Topcu, Arzu Aysan, Şevval Di̇k, Mai̇de Şeker, Meli̇ke Bi̇nnur Bahçekapili, Sude Topkaraoğlu, Di̇lek Yavuzer, Tamer Yağci

Turkish Journal of Biology

Background/aim: HCC is a common and lethal malignancy and multi-kinase inhibitors (MKIs) are among the therapeutic options for unresectable cases. However, response rates to MKIs remained variable, necessitating the identification of predictive biomarkers. Plexin C1 (PLXNC1), a receptor involved in cell signaling, has emerged as a potential candidate to regulate tumor responses. This study aims to evaluate the impact of PLXNC1 expression on the sensitivity of HCC cells to MKI therapy.

Materials and methods: shRNA-mediated PLXNC1 knock-down and control clones of HCC cell lines PLC/PRF/5 and Hep3B were generated, and downregulation of PLXNC1 was confirmed using Western blotting. …

Sustainable Forage Yield Improvement Through Perennial Grass–Legume Intercropping In Degraded Grasslands, Alina-Ștefania Stanciu

Turkish Journal of Agriculture and Forestry

Grasslands constitute one of the largest terrestrial biomes, providing essential ecosystem services such as biodiversity preservation, food production, and climate change mitigation. Globally, the desertification and degradation of grasslands have been accelerated by intensified land use and climate change. It is crucial to conserve and restore grasslands to address climate and biodiversity challenges. However, due to their diverse nature and undervalued benefits for humans, grasslands have often been overlooked in terms of preservation, conservation, and restoration compared to forests. In sustainable agriculture, intercropping grasses with legumes can enhance crop nutritional quality, increase productivity, and impact soil microbiological activity, ultimately leading …