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Articles 271 - 300 of 10915
Full-Text Articles in Life Sciences
Bacteria Recovered From Ear Swab Samples Of Cats With Otitis Externa And Their Antimicrobial Resistance Profiles In Kayseri, Türkiye, Emre Karakaya, Doğancan Yarim, Özgür Güran, Cansu Güran, Umut Alpman, İzzet Burçi̇n Saticioğlu, Gülteki̇n Atalan, Seçi̇l Abay, Fuat Aydin
Bacteria Recovered From Ear Swab Samples Of Cats With Otitis Externa And Their Antimicrobial Resistance Profiles In Kayseri, Türkiye, Emre Karakaya, Doğancan Yarim, Özgür Güran, Cansu Güran, Umut Alpman, İzzet Burçi̇n Saticioğlu, Gülteki̇n Atalan, Seçi̇l Abay, Fuat Aydin
Turkish Journal of Veterinary & Animal Sciences
This study aimed to isolate and identify bacteria from ear swab samples from cats with otitis externa and to detect the antimicrobial resistance profiles of the recovered isolates. For this purpose, ear swab samples from 100 domestic cats that were detected to have otitis externa were used. The samples collected from the animals were inoculated onto 7% defibrinated sheep blood agar and MacConkey agar, and the media were incubated at 37°C for 24-72h under aerobic/microaerobic conditions. The definition of the isolates was carried out by phenotypic tests, MALDI-TOF MS analysis, and 16S rRNA gene sequencing. Antimicrobial susceptibilities of the obtained …
Growth Curve And Serum Calcium And Magnesium Concentrations In Duck Breeders (Anas Boschas) From Hatching To The Prelaying Period Reared Under Subtropical Conditions, Vanessa Peripolli, Fabiano Dahlke, Ellen Karen Dos Santos, Maila Palmeira, Stelamaris Dezen, Robert Lenock, Elizabeth Schwegler, Juahil Martins De Oliveira Júnior, Ivan Bianchi, Paula Gabriela Da Silva Pires, Betina Raquel Cunha Dos Santos, Andressa Fernanda Campos, Fabiana Moreira
Growth Curve And Serum Calcium And Magnesium Concentrations In Duck Breeders (Anas Boschas) From Hatching To The Prelaying Period Reared Under Subtropical Conditions, Vanessa Peripolli, Fabiano Dahlke, Ellen Karen Dos Santos, Maila Palmeira, Stelamaris Dezen, Robert Lenock, Elizabeth Schwegler, Juahil Martins De Oliveira Júnior, Ivan Bianchi, Paula Gabriela Da Silva Pires, Betina Raquel Cunha Dos Santos, Andressa Fernanda Campos, Fabiana Moreira
Turkish Journal of Veterinary & Animal Sciences
Optimizing duck production requires understanding growth variability and monitoring serum calcium and magnesium levelsas health indicators. This study aimed to analyze the growth curve, mortality, and serum calcium (Ca) and magnesium (Mg) levels in White Mallard ducks from hatching to the prelaying period in subtropical conditions. A total of 1100 females and 270 males were raised for 146 days. At hatch, day 6 (D6), D13, D23, D34, D50, D64, D80, D94, D111, and D146, ducks were weighed separately to assess body weight (BW). Blood samples were collected at hatch, D50, D94, and D146 to determine serum calcium and magnesium levels. …
A Morphological Study On Acanthocephalan Species From Wild Birds In Northern Türkiye, Mehmet Öztürk, Şi̇nasi̇ Umur
A Morphological Study On Acanthocephalan Species From Wild Birds In Northern Türkiye, Mehmet Öztürk, Şi̇nasi̇ Umur
Turkish Journal of Veterinary & Animal Sciences
The phylum Acanthocephala contains approximately 1300 acanthocephalan species. So far, only nine acanthocephalan species of wild birds have been reported in Türkiye. To study the context of acanthocephalan diversity in wild birds in Türkiye, we examined 193 birds from 69 species belonging to 16 orders. Acanthocephalans were found in seven bird species: Centrorhynchus aluconis in Buteo buteo and Buteo rufinus, Centrorhynchus spinosus in Buteo buteo and Strix aluco, Mediorhynchus papillosus in Iduna icterina, Mediorhynchus tenuis in Luscinia svecica, Plagiorhynchus cylindraceus in Erithacus rubecula and Turdus merula, Polymorphus contortus in Anas platyrhynchos, and Sphaerirostris turdi in Turdus merula. Except for Plagiorhynchus …
Thionine Modulates Tau Phosphorylation In An Alzheimer’S Disease Cell Culture Model, Seda Önder, Kevser Bi̇beroğlu, Özden Tacal
Thionine Modulates Tau Phosphorylation In An Alzheimer’S Disease Cell Culture Model, Seda Önder, Kevser Bi̇beroğlu, Özden Tacal
Turkish Journal of Biology
Background/aim: Tau protein, which is crucial for sustaining the cytoskeletal network by assisting microtubule construction, contributes significantly to the pathophysiology of Alzheimer’s disease (AD). The hyperphosphorylation of tau causes it to detach from microtubules (MTs), leading to the formation of neurofibrillary tangles (NFTs) in neurons, which ultimately results in cell death. Thionine (TH), a cationic phenothiazine-structured compound, has been the topic of extensive research due to its interesting physicochemical properties. It is a common biological dye, especially useful in histology due to its strong affinity for biological membranes. Furthermore, TH serves as a photosensitizer in phototherapy. It has a phenothiazine …
Different Hypoxic Response Of Human Cavii And Caenorhabditis Elegans Homologous Genes Cah-3, Cah-4, Cah-5, Sümeyye Aydoğan Türkoğlu, Aysu Bozkurt, Fatma Poyrazli, Derya Okuyan
Different Hypoxic Response Of Human Cavii And Caenorhabditis Elegans Homologous Genes Cah-3, Cah-4, Cah-5, Sümeyye Aydoğan Türkoğlu, Aysu Bozkurt, Fatma Poyrazli, Derya Okuyan
Turkish Journal of Biology
Background/aim: A number of carbonic anhydrase (CA) family proteins have been implicated in cancer. They contribute to the hypoxic microenvironment. CAVII is often downregulated in colorectal carcinoma and it has been associated with increased tumor size, node metastasis, and adverse clinical outcomes. In this study, we aimed to investigate the effect of hypoxia on CAVII protein in human colon cancer and prostate cancer cells. In addition, the regulation of CAH genes in Caenorhabditis elegans was examined. These are homologous to CAVII in humans.
Materials and methods: CAVII expression was analyzed in different cell lines such as human colon cancer (SW480 …
Kabasura Kudineer Choornam, A Medicinal Polyherbal Formulation, Modulates Human Macrophage Polarization And Phagocytic Function, Asli Korkmaz, Duygu Ünüvar, Si̇nem Günalp, Derya Göksu Helvaci, Duygu Sağ
Kabasura Kudineer Choornam, A Medicinal Polyherbal Formulation, Modulates Human Macrophage Polarization And Phagocytic Function, Asli Korkmaz, Duygu Ünüvar, Si̇nem Günalp, Derya Göksu Helvaci, Duygu Sağ
Turkish Journal of Biology
Background/aim: Kabasura kudineer choornam (KKC) is a polyherbal formulation of 15 ingredients. It has antiinflammatory and antimicrobial properties and are effective in managing the symptoms of H1N1 swine flu and COVID-19. However, its mechanism of action is not fully understood. In this study, we examined the effect of KKC on the polarization and function of primary human macrophages.
Materials and methods: Human monocyte-derived macrophages (M0 macrophages) pretreated with KKC extract were polarized into M1, M2a, or M2c subtypes. The expression of the M1/M2 polarization markers was analyzed using qPCR, flow cytometry, and ELISA, and the phagocytosis capacity of …
Mir-9-5p Alleviates The Development Of Abdominal Aortic Aneurysm By Regulating The Differentiation Of Cd4+Il-10+T Cells Via Targeting The Crosstalk Between Nrf2 And Nf-Κb Signaling Pathways, Hongfu Liu, Jinyi Zhang, Lubin Li, Benxiang Yu, Chunlei Zhang, Wenqiang Niu, Yawen Cheng, Hengyang Dong, Yukun Zhang, Xinlin Luo, Yanlian Xiong, Yueming Wang
Mir-9-5p Alleviates The Development Of Abdominal Aortic Aneurysm By Regulating The Differentiation Of Cd4+Il-10+T Cells Via Targeting The Crosstalk Between Nrf2 And Nf-Κb Signaling Pathways, Hongfu Liu, Jinyi Zhang, Lubin Li, Benxiang Yu, Chunlei Zhang, Wenqiang Niu, Yawen Cheng, Hengyang Dong, Yukun Zhang, Xinlin Luo, Yanlian Xiong, Yueming Wang
Turkish Journal of Biology
Background: Abdominal aortic aneurysm (AAA), a gradual segmental dilatation of the abdominal aorta, is associated with a high mortality rate. The pathophysiological molecular mechanisms underlying AAA remain unclear. In recent years, changes in miRNA levels have been reported to be involved in the development and treatment of AAA. This study aimed to investigate the potential targets and underlying mechanisms of miR-9-5p in attenuating AAA progression by modulating the inflammatory response.
Materials and methods: Biochemical kits were used to measure the levels of inflammatory factors, antioxidant enzyme activity, and serum oxidative stress in normal and AAA model mice. miR-9-5p overexpression was …
Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang
Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang
Turkish Journal of Biology
Aim: Neuronal cell death plays a critical role in the development of neurological disorders associated with aging. This study aimed to evaluate the beneficial effects of heat-killed lactic acid bacteria (hkLAB) on neuroblastoma cells and Caenorhabditis elegans.
Materials and methods: We pretreated heat-killed Lactobacillus fermentum CNU384 (hkCNU384), L. brevis CNU386 (hkCNU386), L. helveticus CNU395 (hkCNU395), and L. paracasei CNU396 (hkCNU396) at a concentration of 109 CFU/mL to investigate their neuroprotective effects on glutamate-induced SH-SY5Y cells and their impact on the lifespan of 6-hydroxydopamine (6-OHDA)-induced C. elegans.
Results: Our findings indicate that hkCNU395 and hkCNU396 protected against glutamate-induced cell death …
Chelidonium Majus L.-Containing Gel May Improve Diabetic Wound Healing By Modulating Mmp-2, Mmp-9, And Collagen Levels, Kaan Kaltalioğlu, Eli̇f Naz Gürsoy, Barbaros Balabanli, Zeki̇ Aytaç, Şule Coşkun Cevher
Chelidonium Majus L.-Containing Gel May Improve Diabetic Wound Healing By Modulating Mmp-2, Mmp-9, And Collagen Levels, Kaan Kaltalioğlu, Eli̇f Naz Gürsoy, Barbaros Balabanli, Zeki̇ Aytaç, Şule Coşkun Cevher
Turkish Journal of Biology
Background/aim: Delayed wound healing in diabetic patients is a significant complication that reduces quality of life, prompting the continuous investigation of new therapeutic agents. This study designed to explore the dose-dependent effects of different parts of Chelidonium majus L. (CM), a medicinal plant traditionally used for skin disorders, on diabetic skin wounds.
Materials and methods: In diabetic rats, full-thickness excisional wounds were formed. CM-containing gels (aerial parts at 3%, 6%, 9% and root at 0.5%, 1%, 1.5%) were developed and applied to the wounds. After the treatment period, the rats were sacrificed, and wound healing activity was assessed macroscopically, histopathologically, …
Anatomical And Palynological Studies On Turkish Primula Taxa (Primulaceae) And Their Taxonomic And Phylogenetic Significance, Deni̇z Ulukuş, Esengül Özkaymakoğlu, Rebecca Stubbs, Hüseyi̇n Baykal, Kami̇l Coşkunçelebi̇, Osman Tugay, Barbara Keller, Elena Conti, Ferhat Celep
Anatomical And Palynological Studies On Turkish Primula Taxa (Primulaceae) And Their Taxonomic And Phylogenetic Significance, Deni̇z Ulukuş, Esengül Özkaymakoğlu, Rebecca Stubbs, Hüseyi̇n Baykal, Kami̇l Coşkunçelebi̇, Osman Tugay, Barbara Keller, Elena Conti, Ferhat Celep
Turkish Journal of Botany
Anatomical (scape, pedicel, leaf lamina, and leaf midrib) and pollen morphological features of 11 taxa belonging to the genus Primula subgen. Primula (P. vulgaris subsp. vulgaris, P. vulgaris subsp. rubra, and P.megaseifolia, P. meyeri,P. elatior subsp. pallasii, P. pseudoelatior, P. veris subsp. columnae, P. veris subsp. macrocalyx) and subgen. Aleurita (P. longipes, P. algida and P. auriculata) were examined using light and scanning electron microscopy to shed light on the existing taxonomic and phylogenetic relationships. The anatomical and palynological features of the majority of the taxa studied were revealed for the first time in this study. In anatomical studies, the …
Comparative Mitogenomics Of Clitellata Reveals Mitogenome Organization Can Affect The Mode Of Phylogeny, Deni̇z Mercan, Austin Horenkamp, Merve Nur Aydemi̇r, Habeş Bi̇lal Aydemi̇r, Kurt Neubig, Nai̇me Arslan, Frank E. Anderson
Comparative Mitogenomics Of Clitellata Reveals Mitogenome Organization Can Affect The Mode Of Phylogeny, Deni̇z Mercan, Austin Horenkamp, Merve Nur Aydemi̇r, Habeş Bi̇lal Aydemi̇r, Kurt Neubig, Nai̇me Arslan, Frank E. Anderson
Turkish Journal of Zoology
Clitellata is a major clade of Annelida that includes almost all freshwater and terrestrial annelids as well as many marine species. Aquatic oligochaetes include the union of the two multispecies families: Naididae and Tubificidae. There has been much debate regarding the phylogeny and classification of the cosmopolitan and diverse Naididae. In this study, the total mitogenome sequences of Potamothrix hammoniensis (Naididae: Tubificinae), Stylaria fossularis, Stylaria lacustris, Chaetogaster diaphanus, Chaetogaster diaphanus sp. B, and Slavina appendiculata (Naididae: Naidinae) were sequenced and characterized and can be accessed from NCBI under accession numbers OQ654101, PP909790, PP909791, PP909792, PP909793, and PP893275, respectively. For comparative …
Insights Into Therapeutic Discovery Through The Kelch Domain Structure Of Keap1 At Ambient Temperature, Merve Yilmaz, Belgi̇n Sever, Yi̇ği̇t Kutlu, Mehmet Gül, Ceren Okuducu, Serra Tavli, Masami Otsuka, Mikako Fujita, Turkan Hali̇loğlu, Hali̇li̇brahi̇m Çi̇ftçi̇, Hasan Demi̇rci̇
Insights Into Therapeutic Discovery Through The Kelch Domain Structure Of Keap1 At Ambient Temperature, Merve Yilmaz, Belgi̇n Sever, Yi̇ği̇t Kutlu, Mehmet Gül, Ceren Okuducu, Serra Tavli, Masami Otsuka, Mikako Fujita, Turkan Hali̇loğlu, Hali̇li̇brahi̇m Çi̇ftçi̇, Hasan Demi̇rci̇
Turkish Journal of Biology
Background/aim: The Kelch-like-ECH associated protein 1 (Keap1) is an integral component of the E3-ubiquitin ligase complex, which binds to Nuclear factor erythroid 2-related factor 2 (Nrf2) and facilitates its degradation by the 26S proteasome. The Kelch domain of Keap1, composed of six repeated structural motifs, plays a key role in this interaction. This study aims to investigate the dimeric structure of the Keap1 Kelch domain at ambient temperature and to examine its implications for conformational dynamics, particularly in relation to the DMF and Nrf2 binding sites.Materials and methods: The dimeric crystal structure of the Keap1 Kelch domain was determined at …
Antiinflammatory Effects Of Cucurbitacins And Sorafenib In Hepg2 Cells By Modulating The Iκb/Nf-Κb/Cox-2 Pathway Through Akt Signaling, Muhammed Mehdi̇ Üremi̇ş, Yusuf Türköz, Nuray Üremi̇ş
Antiinflammatory Effects Of Cucurbitacins And Sorafenib In Hepg2 Cells By Modulating The Iκb/Nf-Κb/Cox-2 Pathway Through Akt Signaling, Muhammed Mehdi̇ Üremi̇ş, Yusuf Türköz, Nuray Üremi̇ş
Turkish Journal of Biology
Aim: Cucurbitacins possess antitumor, antiproliferative, and antiinflammatory properties. This study aims to examine the antiinflammatory effects of CuD, CuI, and CuE on NF-κB, iNOS, COX-2, and Akt and compare their cytotoxic and antiinflammatory effects on HepG2 cells with sorafenib, the primary chemotherapeutic agent used in HCC treatment. Methods: Cytotoxic effects of cucurbitacins and sorafenib on HepG2 cells were evaluated using MTT and LDH assays, along with Annexin V, MMP, and comet assays. Levels of proteins related to the Akt/NF-κB pathway and COX-2, iNOS, and NO were also measured. Results: Our study showed that CuD, CuI, CuE, and sorafenib have antiproliferative …
Fda-Approved Drugs As Potential Covalent Inhibitors Of Key Sars-Cov-2 Proteins: An In Silico Approach, Murat Seri̇lmez, Anwar Abuelrub, İsmai̇l Erol, Serdar Durdaği
Fda-Approved Drugs As Potential Covalent Inhibitors Of Key Sars-Cov-2 Proteins: An In Silico Approach, Murat Seri̇lmez, Anwar Abuelrub, İsmai̇l Erol, Serdar Durdaği
Turkish Journal of Biology
Background/aim: The COVID-19 pandemic caused by SARS-CoV-2 necessitated rapid development of effective therapeutics, prompting this study to identify potential inhibitors targeting key viral and host proteins: RNA-dependent RNA polymerase (RdRp), main protease (Mpro), transmembrane serine protease 2 (TMPRSS2), and angiotensin-converting enzyme 2 (ACE2). Methods: We used covalent docking and molecular dynamics (MD) simulations to screen FDA-approved compounds against these targets using diverse covalent reaction mechanisms. Top-ranking compounds underwent further evaluation through MD simulations to assess binding stability and conformational dynamics. Results: Several promising drug repurposing candidates were identified: bremelanotide, lanreotide, histrelin, and leuprolide as potential RdRp inhibitors; azlocillin, cefiderocol, and …
Screening Of Ccdc43 Molecular Partners By Bioid2-Based Proximity Labeling, Merve Tuzlakoğlu Öztürk
Screening Of Ccdc43 Molecular Partners By Bioid2-Based Proximity Labeling, Merve Tuzlakoğlu Öztürk
Turkish Journal of Biology
Background/aim: CCDC43 (coiled-coil domain containing protein 43) is a eukaryotic protein that contains alpha-helical domains in its structure, consistent with the family to which it belongs. It is predominantly located in the cytosol of the cell. CCDC43 protein has been shown to play a role in cell proliferation, invasion, metastasis, and epithelial–mesenchymal transition in gastric and colorectal cancer types. The fundamental function and cellular interaction network of this protein, which is known to have varying expression levels in various cancer types, has not yet been fully elucidated. Here, we conducted a proximity-dependent biotin identification (BioID2) screening approach for CCDC43 to …
Butyrylcholinesterase (Bche) Downregulation In Taxane Resistance: Implications For Prostate Cancer, Buse Cevatemre, Arda Işiklar, İpek Bulut, Ezgi̇ Karyemez, Hamzah Syed, Ceyda Açilan
Butyrylcholinesterase (Bche) Downregulation In Taxane Resistance: Implications For Prostate Cancer, Buse Cevatemre, Arda Işiklar, İpek Bulut, Ezgi̇ Karyemez, Hamzah Syed, Ceyda Açilan
Turkish Journal of Biology
Background/aim: Despite advancements in chemotherapeutic strategies, drug resistance remains a major barrier to effective cancer treatment. While primary prostate cancers (PC) can be treated with surgery and radiotherapy, treatment options for recurrent PC are limited. Upon progression to the castration-resistant (CR) phase, therapies rely on taxanes (chemotherapeutic drugs) like docetaxel (Dtx) and cabazitaxel (Cbz); however, resistance to taxanes is common in CRPC, highlighting the importance of identifying underlying molecular mechanisms or targets in resistant cells.Materials and methods: In transcriptome (RNA sequencing) analyses comparing taxane-resistant PC cells (resistant to both Dtx and Cbz) with parental (nonresistant, sensitive) cells, butyrylcholinesterase (BChE) was …
Proteomic Profiling Of Escherichia Coli Cytoplasmic Proteins Under Sublethal Boric Acid Stress, Beki̇r Çöl, Begüm Hazar Çi̇ftçi̇, Merve Sezer Kürkçü, Esra Di̇bek
Proteomic Profiling Of Escherichia Coli Cytoplasmic Proteins Under Sublethal Boric Acid Stress, Beki̇r Çöl, Begüm Hazar Çi̇ftçi̇, Merve Sezer Kürkçü, Esra Di̇bek
Turkish Journal of Biology
Background/aim: Boron is an essential micronutrient for plants and certain bacteria, where it plays critical roles in cellular processes at low concentrations. However, elevated levels of boron-containing compounds, such as boric acid, exhibit antimicrobial toxicity. Although the physiological effects of boric acid on bacteria have been partially characterized, its proteome-wide impacts remain poorly elucidated. This study employs a 2D-PAGE-based proteomic approach to investigate how sublethal boric acid stress alters the cytoplasmic proteome of Escherichia coli BW25113. Materials and methods: E. coli BW25113 cultures were grown to mid-log phase in tryptic soy broth (TSB) and exposed to 70 mM boric acid …
Novel Mrsa-Targeting Phage Metb16: Genomic Features, Structural Insights, And Therapeutic Applications, Berna Erdoğdu, Senanur Dokuz, Görkem Güngör, Wei Lin, Yigang Tong, Tüli̇n Özbek
Novel Mrsa-Targeting Phage Metb16: Genomic Features, Structural Insights, And Therapeutic Applications, Berna Erdoğdu, Senanur Dokuz, Görkem Güngör, Wei Lin, Yigang Tong, Tüli̇n Özbek
Turkish Journal of Biology
Background/aim: Recent reports have indicated that multidrug-resistant strains of S. aureus, including methicillin-resistant strains, may pose a significant threat to public health and global economic stability. Materials and methods: In this study, we present the isolation and comprehensive characterization of a novel phage, derived from clinically isolated MRSA strains. Results: MetB16 exhibited an incubation period of approximately 20 min, a lysis period of around 45 min, and a burst size of 127 Plaque Forming Units (PFU)/cell. The phage demonstrated remarkable biological stability across a pH spectrum of 4.0–9.0 and maintained integrity within a temperature range of 37 and –80 °C. …
Discovery And Characterization Of Selective Lipase-Inhibiting Polyheterocyclic Derivatives: A Combined In Silico And In Vitro Study, Madjda Benguechoua, Mebarka Imane Benguechoua, Khedidja Benarous, Houssem Boulebd, Ibtissem Kadi, Ari̇f Mermer, Yakup Şi̇ri̇n, Alaeddine Kaouka, Mohamed Yousfi
Discovery And Characterization Of Selective Lipase-Inhibiting Polyheterocyclic Derivatives: A Combined In Silico And In Vitro Study, Madjda Benguechoua, Mebarka Imane Benguechoua, Khedidja Benarous, Houssem Boulebd, Ibtissem Kadi, Ari̇f Mermer, Yakup Şi̇ri̇n, Alaeddine Kaouka, Mohamed Yousfi
Turkish Journal of Biology
Background/aim: Obesity has become a global health crisis with an increasing prevalence, necessitating the search for effective therapies. Schiff base derivatives, known for their broad pharmacological activities, have gained attention as potential antiobesity agents. This study aimed to investigate the lipase inhibitory potential of novel Schiff base derivatives and assess their drug-like properties through in vitro assays and in silico methods. Materials and methods: The lipase inhibitory activity of synthesized Schiff base derivatives was evaluated using in vitro assays, with IC50 values determined for each compound. Additionally, in silico ADMET predictions (absorption, distribution, metabolism, excretion, and toxicity), molecular docking studies, …
The Effects Of Hsa-Mir-26a-5p On Cell Proliferation, Migration, And Pi3k Inhibitor Sensitivity In Metformin-Resistant Triple Negative Breast Cancer Cells, Şahi̇ka Cingir Köker, Senem Noyan, Banu Yalçin, İrem Doğan Turaçli
The Effects Of Hsa-Mir-26a-5p On Cell Proliferation, Migration, And Pi3k Inhibitor Sensitivity In Metformin-Resistant Triple Negative Breast Cancer Cells, Şahi̇ka Cingir Köker, Senem Noyan, Banu Yalçin, İrem Doğan Turaçli
Turkish Journal of Biology
Background/aim: Metformin is commonly used to manage type 2 diabetes (T2D) and is being investigated for its potential antiproliferative effects in cancer, particularly in patients with both T2D and malignancies. Drug resistance can develop with any therapeutic agent, and metformin is no exception. As we showed in our previous study, metformin-resistant MDA-MB-468 (MET-R) cells exhibited an EMT-like phenotype. Many transcription factors, as well as miRNAs, can contribute to this altered phenotype. Our current study identifies the contribution of hsa-miR-26a-5p expression to the previously observed phenotype. Materials and methods: By utilizing bioinformatic tools, we identified hsa-miR-26a-5p, whose expression was significantly altered …
Expression Of Concern: Characterization Of Tfiie-Regulated Genes By Transcriptomeanalysis, Serdar Baysal
Expression Of Concern: Characterization Of Tfiie-Regulated Genes By Transcriptomeanalysis, Serdar Baysal
Turkish Journal of Biology
Update: This Expression of Concern has been superseded by a Retraction Notice. The Expression of Concern remains part of the permanent publication record. Please see the Retraction Notice and the original article.
The Editors of Turkish Journal of Biology wish to issue an Expression of Concern regarding the article entitled: “Characterization of TFIIE-regulated genes by transcriptome analysis” by Serdar Baysal published on 20.12.2024. Following the publication of this article, concerns have been raised regarding• Potential violations of academic ethical standards in the published study• Potential intellectual property infringement, including plagiarism• An academic sanction reportedly imposed on the author by …
In Vitro Regeneration Potential Of Pistacia Integerrima And Pistacia Khinjuk Rootstocks: A Modeling Approach For Optimal Medium Formulation, Heydem Eki̇nci̇, Beki̇r Erol Ak
In Vitro Regeneration Potential Of Pistacia Integerrima And Pistacia Khinjuk Rootstocks: A Modeling Approach For Optimal Medium Formulation, Heydem Eki̇nci̇, Beki̇r Erol Ak
Turkish Journal of Agriculture and Forestry
Micropropagation involves the development of whole plants from small meristematic tissues by culturing them in vitro under aseptic conditions on a nutrient medium. However, identifying a single nutrient medium suitable for propagating Pistacia, a recalcitrant species, remains challenging. While some species exhibit good growth on Murashige and Skoog (MS) medium, Pistacia species generally yield insufficient results. To address this, a five-dimensional experimental design was developed using Design- Expert software (Stat-Ease Inc., Minneapolis, MN, USA). Thirty-three experimental databases were generated by the software using the modified I-optimal criterion. MS medium served as the control treatment. A response surface model (RSM) employing …
Deep Reinforcement Learning-Driven Decision Support System Design For Precision Agriculture: Optimizing Farming And Resource Use, Qingshan Bai
Turkish Journal of Agriculture and Forestry
This study focuses on enhancing the efficiency of agricultural decision support systems by integrating deep reinforcement learning techniques to optimize the path planning of agricultural vehicles. By decomposing the system into dynamics, action, strategy, reward, and discount factor components, a robust framework is established. A value-based reinforcement learning algorithm is introduced, enriched with policy gradient (PG) methodology to enable continuous and adaptive state-to-action decision-making process. A deep neural network (DNN) was used to approximate the state-action value function, resulting in the innovative DNN-PG-Q-learning algorithm. This algorithm leverages an empirical playback pool to enable efficient and intelligent path planning for agricultural …
Accurate Crop Yield Prediction Via Temporal Convolutional Network And Variational Autoencoder, Lei Liu, Xiaoling Song
Accurate Crop Yield Prediction Via Temporal Convolutional Network And Variational Autoencoder, Lei Liu, Xiaoling Song
Turkish Journal of Agriculture and Forestry
With the advancement of technology, smart agriculture assumes a pivotal role in enhancing the efficiency and sustainability of agricultural production. Nevertheless, accurately forecasting crop yields and understanding their determining factors often faces limitations inherent in conventional data processing techniques. To address this challenge, this study proposes a TCN-VAE framework, amalgamating temporal convolutional networks (TCNs) and variational autoencoders (VAEs), to tackle the crop yield prediction task in smart agricultural systems. The framework intricately models four principal categories of features—meteorological conditions, soil conditions, agricultural management, and environmental conditions—via TCN and compensates for missing data using VAE, thereby achieving a comprehensive analysis and …
Comprehensive Characterization Of Purified Naringinase: Effect On Naringin Content, Antioxidant Activity, Phenolic Profile, And Grapefruit Juice Quality, Arzu İmece, Memnune Şengül, Bülent Çeti̇n, Ömer İrfan Küfrevi̇oğlu, Arzu Öztürk Kesebi̇r
Comprehensive Characterization Of Purified Naringinase: Effect On Naringin Content, Antioxidant Activity, Phenolic Profile, And Grapefruit Juice Quality, Arzu İmece, Memnune Şengül, Bülent Çeti̇n, Ömer İrfan Küfrevi̇oğlu, Arzu Öztürk Kesebi̇r
Turkish Journal of Agriculture and Forestry
Bitterness arising from naringin is a problem in citrus juices, especially grapefruit juice, in terms of consumer preference. Naringinase enzyme is used to eliminate the bitterness. In this study, naringinase was extracellularly extracted, isolated, and characterized from an Aspergillus niger strain in different media. The extracted enzyme was added to grapefruit juice at different concentrations (0.1%, 0.5%, 1%) (0.078, 0.39 and 0.78 EU) and stored at different temperatures (25, 35, and 45 °C) for 5 h. At the end of the first and fifth h, the amount of naringin, physicochemical properties, phenolic profiles, and antioxidant activities of the grapefruit juice …
Human Settlement Environment And Architectural Ecological Design Based On Artificial Intelligence Technology, Mingxing Xu, Xiongjun Tao
Human Settlement Environment And Architectural Ecological Design Based On Artificial Intelligence Technology, Mingxing Xu, Xiongjun Tao
Turkish Journal of Agriculture and Forestry
The main purpose of this paper was to use artificial intelligence (AI) technology to improve the coordination between architectural design and the natural environment, so as to optimize resource utilization and reduce negative environmental impacts and improve the quality of human settlements. To do this, multiple types of data covering the environment, buildings, and user feedback were first collected and preprocessed. Based on these data, the long short-term memory model was used to predict future environmental changes, and the particle swarm optimization algorithm was used for multiobjective optimization of architectural design. The experimental results showed that the AI-driven design scheme …
The Effects Of Melatonin And Pgpr Applications On Phenolic Compounds, Organic Acids, And Agromorphological Characteristics In Strawberry (Fragaria × Ananassa Duch.) Fruits By Multivariate Analysis, Akgül Taş
Turkish Journal of Agriculture and Forestry
In this study, melatonin, plant growth promoting rhizobacteria (PGPR), and PGPR + melatonin were applied to the Festival and Kabarla strawberry varieties and the effects of these applications on fruit quality were investigated. The parameters of fruit weight, fruit width and length, soluble solids content (SSC), pH, titratable acidity (TA), phenolic compounds, organic acids, and vitamin C parameters were examined. The analysis shows that significantly better results were obtained in terms of fruit weight, fruit width, fruit length, and SSC values in all fruits treated with melatonin, PGPR, and PGPR + melatonin, although there was variation between the strawberry varieties. …
Application Of Vermicompost Extract To Improve The Phytochemical Composition Of Berry Fruits, Jelena Tomić, Žaklina Karaklajić-Stajić, Marijana Pešaković, Svetlana M. Paunović, Boris Rilak, Maja Mikulic-Petkovsek, Metka Hudina, Robert Veberic, Nesi̇be Ebru Kafkas, Sezai̇ Erci̇şli̇, Jerneja Jakopic
Application Of Vermicompost Extract To Improve The Phytochemical Composition Of Berry Fruits, Jelena Tomić, Žaklina Karaklajić-Stajić, Marijana Pešaković, Svetlana M. Paunović, Boris Rilak, Maja Mikulic-Petkovsek, Metka Hudina, Robert Veberic, Nesi̇be Ebru Kafkas, Sezai̇ Erci̇şli̇, Jerneja Jakopic
Turkish Journal of Agriculture and Forestry
The growing market demand for environmentally friendly products for plant nutrition has led to the development of new fertilizers, particularly liquid compost-based products such as teas, extracts, and leachates. The aim of this research was to compare the effect of chemical fertilization (CF) and its combination with liquid fertilizer based on vermicompost and beneficial microorganisms (CF + VP) on the phytochemical profile of strawberry (Senga Sengana), blackberry (Čačanska Bestrna) and blueberry (Aurora). The results showed that CF + VP positively affected fruit weight (8.8 g), soluble solids (8.9°Bx), total sugars (65.5 g/kg FW), and the sum of all phenolic groups …
Green Biosynthesis Of Cobalt Oxide Nanoparticles Using Saccharum Munja Leaf Extract As A Catalyst For Callus Induction In Carnation (Dianthus Caryophyllus L.) Plants, Ishtiaq Ahmad, Sameed Arshad Chaudhry, Maryam Maryam, Aysha Arif Chahel, Riaz Ahmad, Mehmet Ramazan Bozhüyük, İbrahi̇m Bozkurt, Magda Abdel-Rahman, Konstantin Korolev
Green Biosynthesis Of Cobalt Oxide Nanoparticles Using Saccharum Munja Leaf Extract As A Catalyst For Callus Induction In Carnation (Dianthus Caryophyllus L.) Plants, Ishtiaq Ahmad, Sameed Arshad Chaudhry, Maryam Maryam, Aysha Arif Chahel, Riaz Ahmad, Mehmet Ramazan Bozhüyük, İbrahi̇m Bozkurt, Magda Abdel-Rahman, Konstantin Korolev
Turkish Journal of Agriculture and Forestry
The common name of a wild grass native to Pakistan with both edible and medicinal properties is Saccharum munja Roxb. (S. munja). Prior research has investigated various plant species for the green biosynthesis of nanoparticles to circumvent issues related to their physical and chemical production. This study presents the first evidence of synthesizing cobalt oxide nanoparticles (Co3O4NPs) using S. munja leaf extract (SMLE) as both a reducing and a capping agent. This study investigated their ability to induce in vitro callus formation in Dianthus caryophyllus L. (carnation). In this study, Co3O4NPs were synthesized using varying quantities of SMLE to achieve …
Precision Crop Yield Prediction Model Based On Deep Learning And Multimodal Data Fusion, Zijin Mo, Yanxiong Wu
Precision Crop Yield Prediction Model Based On Deep Learning And Multimodal Data Fusion, Zijin Mo, Yanxiong Wu
Turkish Journal of Agriculture and Forestry
More precise crop yield prediction methods are essential in advancing precision agriculture, ensuring food security, and promoting sustainable agricultural practices. Deep learning technologies have significantly improved prediction accuracy by allowing the analysis of large-scale agricultural datasets. However, existing approaches still face challenges in effectively integrating multisource data, such as meteorological, soil, and remote sensing information, and optimizing model parameters. To address these issues, this study introduces TCGNet, a novel deep learning architecture that combines a time convolutional network (TCN), convolutional long short-term memory (ConvLSTM), and the grey wolf optimization (GWO) algorithm. The TCN is employed for time series analysis, ConvLSTM …