Incorporation Of Biorationals And Trap Crop For Stink Bug Management In Soybeans, Kukuh Hernowo

LSU Doctoral Dissertations

Stink bugs are one of the most important seed sucking pests impacting soybean production in Louisiana. Exploration of chemicals that exhibit attraction or repellent activities toward this major pest would be beneficial to develop a push-pull strategy against this pest complex. Spinosad and neem are two commercially available natural insecticides that were claimed to have attractant and repellent activities against stink bugs. To test the potential of these chemicals, a series of experiments was conducted to investigate the olfactory, tactile, feeding, and oviposition preference of stink bugs toward different commercial spinosad products and neem. Additionally, two years of field experiments …

Sweetpotato Virus C And Its Contribution To The Potyvirus Complex In Sweetpotato (Ipomoea Batatas), Favio E. Herrera Eguez

LSU Doctoral Dissertations

In Louisiana, sweetpotato (Ipomoea batatas) is infected in Louisiana by the four ubiquitous potyviruses: Sweetpotato feathery mottle virus (SPFMV), Sweetpotato virus G (SPVG), Sweetpotato virus 2 (SPV2) and the strain of SPFMV previously known as the common strain, recently renamed as Sweetpotato virus C (SPVC). These four viruses belong to the Potyviridae family, with single stranded RNA of ~11kb. In this group of plant viruses, a single polyprotein is coded entirely but later cleaved into ten mature proteins: P1, HC-pro, P3, 6K1, CI, 6K2, NIa-VPg, NIa-Pro Nib and Coat Protein (CP). In sweetpotato potyviruses, two additional open reading …

Alteration Of Gut Microbiota And Other Properties In Zucker Diabetic Fatty Rats After Antibiotics, Diet And Donor Transplant, Ryan Page

LSU Master's Theses

The compositions of bacterial communities inhabiting the human gut are associated with regulating energy balance, immune response, and neurological functions. Many of these benefits are attributed to byproducts of bacteria that feed on fermentable fibers. Research has shown that obese or lean host can transfer their phenotype to germ-free recipients, via microbiota transplant, without alteration in recipient diet. However, transfer may be attenuated by established microbial communities in conventionally-raised rodents or other species. A knockdown of recipient communities may be required before a transplant for microbiota of donors to populate gut of recipient. This study examined whether obese Zucker Diabetic …

Effect Of Hot Water Conditioning On Microbial Safety And Quality Of In-Shell Pecans, Karuna Kharel

LSU Master's Theses

Hot water conditioning of in-shell pecans is one common practice followed by industries to facilitate shelling. It also acts as a preventive control for potential microbiological contamination that might occur during pre- and post-harvest processes. However, heat treatment may have an effect on the eating quality of nuts. The main objectives of the study were to develop a post-harvest hot water treatment intervention as a kill step to destroy foodborne pathogens on in-shell pecans and evaluate the effect of treatments on physicochemical properties, consumer acceptance and purchase intent of dehulled and roasted pecans. The time (1-5 min) and temperature (70, …

Ecology And Biology Of The Redbanded Stink Bug, Piezodorus Guildinii (Westwood) In Louisiana, Anup Bastola

LSU Doctoral Dissertations

The redbanded stink bug, Piezodorus guildinii (Westwood) is an invasive stink bug species that was first documented as a soybean pest in Louisiana during the year 2000. This species continues to expand northward in the MidSouth but information is lacking on its biology and ecology in the U.S. In order to determine P. guildinii’s northern range, studies were designed to investigate the cold tolerance ability of this species. The mean supercooling points of adult P. guildinii ranged from highest -8.3 ± 0.2°C in March to the lowest of -11.0 ± 0.2°C in January. Evaluation of lethal exposure time …

The Role Of Histone H2a.Z Abundance In Modulating Responses To Phosphorus Deficiency In Rice, Sara Zahraeifard

LSU Doctoral Dissertations

Histone variants contribute to chromatin complexity by creating specialized nucleosomes. Here, to investigate the pattern of H2A.Z histone variant distribution and its role in modulating gene expression in rice (Oryza sativa L.), we performed genome-wide profiling of ARP6-dependent H2A.Z deposition in rice seedlings. We demonstrated that under controlled conditions, the majority of H2A.Z deposition is within protein-coding genes (PCG), and the most highly expressed genes are enriched with H2A.Z at their 5’ ends near the transcription start site (TSS), whereas the medium- to low-expressed genes contain H2A.Z across the entire gene. Based on H2A.Z deposition, we categorized genes into …

Biased Genetic Screen Identifies Novel Genes Involved In Antiviral Defense, Tianyun Long

LSU Doctoral Dissertations

ABSTRACT

RNA interference (RNAi) mediates potent antiviral response across kingdoms. In Caenorhabditis elegans nematodes, antiviral RNAi requires a virus sensor that is conserved in mammals and is amplified by secondary small interfering RNAs that are produced in a Dicer-independent manner.

To better understand worm antiviral RNAi, I carried out a biased genetic screen, aiming to identify novel antiviral RNAi genes. To speed up the gene discovery process, the reporter worms used for this genetic screen were engineered to contain extra copies of 4 known antiviral RNAi genes. Therefore, genetic alleles derived from these 4 genes will be automatically rejected during …

The Characterization Of Alpha-Carbonic Anhydrases Through Mutagenesis And Subcellular Localization In Arabidopsis Thaliana, Lance E. Pounds

LSU Master's Theses

Carbonic anhydrases (CAs) are zinc metalloenzymes which catalyze the interconversion of carbon dioxide and bicarbonate. It is hypothesized that CA activity facilitates CO₂ flux in leaf mesophyll cells to maintain optimal rates of photosynthesis or to maintain a pool of inorganic carbon for other metabolic processes. There are three classes of CAs (α, β, and γ) in the model plant Arabidopsis thaliana. The βCAs and γCAs have been well studied, but the αCAs have received relatively little study. There are eight αCA genes in Arabidopsis. This study investigates which αCAs are expressed in shoots, their tissue-specific expression, their …

Incidence Of Aphelenchoides Besseyi In Rice In Louisiana And Host Status Of The Most Widely Planted Cultivars, Felipe Mendes Carvalho Godoy

LSU Master's Theses

Aphelenchoides besseyi, the causal agent of white tip disease of rice, has been considered a minor pest of rice during the past 50 years in the United States. Recently this nematode has been found in a number of quarantine samples in Louisiana and Arkansas. Objectives of this research were to determine incidence of this nematode in commercial seed sold to producers in Louisiana and to determine the host status of major cultivars currently produced in the state. During 2015-2016, a total of 216 seed samples representing 3 medium grain, 18 long grain, and 4 long grain hybrid cultivars were …

Heat, Acid And Osmotic Stress Tolerance Of Leuconostoc Mesenteroides As Influenced By Prior Exposure To Various Mild Stress Conditions, Ingrid C. Osorio Rodriguez

LSU Master's Theses

The importance of Leuconostoc mesenteroides is recognized for its contribution to taste in cultured dairy products. It has been cited for its potential role as a probiotic. Consumer demand exists for new dairy products with health benefits. In the manufacture of probiotic-products survival of Leuconostoc mesenteroides would depend on its ability to tolerate challenging processing and storage conditions. Improved viability by prior exposure to mild stresses can increase stress tolerance toward a more severe stress. Furthermore, it can result in cross-protection due to connection of several proteins in response to stresses. Objective was to evaluate the effect of prior …

Virulence Phenotypes Of Rotylenchulus Reniformis: Evaluation Of Host Status Of Cotton And Utility Of Single Nucleotide Polymorphisms (Snps) For Identification, Churamani Khanal

LSU Doctoral Dissertations

Comparative reproduction and pathogenicity of reniform nematode (Rotylenchulus reniformis) populations derived from single-egg masses and collected form West Carroll (WC), Rapides (RAP), Morehouse (MOR), and Tensas (TEN) parishes in Louisiana were evaluated in microplot and greenhouse trials. Data from microplot trials showed significant differences among isolates of reniform nematode in both reproduction and pathogenicity on upland cotton (Gossypium hirsutum) cultivars Phytogen 499 WRF, Deltapine 1133 B2RF, and Phytogen 333 WRF. Across all cotton cultivars, MOR and RAP isolates had the greatest and the least reproduction values of 331.8 and 230.2, respectively. Reduction in plant dry weight, …

Pathogenicity And Reproduction Of Isolates Of Reniform Nematode, Rotylenchulus Reniformis, From Louisiana On Soybean And Utility Of Single Nucleotide Polymorphisms To Evaluate Genetic Variability, Herath Mudiyanselage Kularathna

LSU Doctoral Dissertations

Experiments were conducted to evaluate soybean, Glycine max (L.) Merr., responses to indigenous isolates of the reniform nematode (Rotylenchulus reniformis) in Louisiana and to understand the genetic variability of these native isolates. Microplot and greenhouse experiments were conducted to evaluate the comparative reproduction and pathogenicity of single egg-mass populations of R. reniformis isolated from West Carroll (WC), Rapides, Tensas and Morehouse (MOR) parishes of Louisiana. Data from full-season microplot trials, displayed significant differences in reproduction and pathogenicity of the nematode with the commercial soybean cultivars REV 56R63, Pioneer P54T94R, and Dyna-Gro 39RY57. Significantly low population density was observed …

Evaluation Of Winter Management Methods To Enhance Survival Of The Giant Salvinia Weevil, Cyrtobagous Salviniae, Lori Robyn Moshman

LSU Master's Theses

Giant salvinia (Salvinia molesta Mitchell) is a noxious aquatic weed in the southeastern United States. Dense plant mats create ecological and economic consequences by displacing native species and impacting freshwater industries. Biological control programs using the salvinia weevil (Cyrtobagous salviniae Calder and Sands, Coleoptera: Curculionidae) have limited efficacy in giant salvinia’s northernmost range due to the weevil’s low cold tolerance and long-term establishment rates. Spunbonded polypropylene (SBP) fabrics and manipulation of plant density were evaluated as two strategies for managing giant salvinia mats during winter. Greenhouse, laboratory, and field experiments were conducted to determine the suitability of these …

Multigeneration Effects Of Chronic Exposure To Polycyclic Aromatic Hydrocarbons In Gulf Killifish (Fundulus Grandis), Andrea Yammine

LSU Master's Theses

The goal of this study was to examine potential adaptation within two generations of Gulf killifish exposed to polycyclic aromatic hydrocarbons (PAH) while also investigating the use of mucus as an effective and non-lethal alternative to determine concentrations of 11-ketotestosterone (11-KT) and vitellogenin (VTG) of F. grandis. Multiple biomarkers were used to assess adaptation within these fish, such as lysozyme (enzyme critical in innate immunity), and 11-ketotestosterone and vitellogenin (endocrine hormones often affected by PAHs).

Mucal concentrations of 11-KT and VTG were determined and evaluated for effectiveness through comparative assays with blood plasma. This method was then applied to …

Interaction Of Cocoa Powder With Intestinal Microbiota, Martha M. Escoto Sabillon

LSU Master's Theses

Cocoa is the fully fermented and dried seed of the cacao tree (Theobroma cacao L.) which has prebiotic properties, due to their high concentration of polyphenols. Therefore, the ingestion of cocoa could cause changes in the proportions of the intestinal microbiota that can influence the intestinal immune response. The objective of this study was to determine the effect of alkalization process of the cocoa bean in the diversity of the gut microbiota. The samples were “lavado” unprocessed cocoa powder, “natural” unprocessed cocoa powder, “D-11-S” as alkalized cocoa powder, “D-11-B” heavily alkalized cocoa powder, and raw cocoa “shells” and a control …

Giant Salvinia, Salvinia Molesta (Salviniaceae): Evaluation Of Sub-Optimum Temperatures On Survival Of The Giant Salvinia Weevil, Cyrtobagous Salviniae (Coleoptera: Curculionidae) And Integration Of Management Practices With Aquatic Herbicides, Lauren W. Cozad

LSU Master's Theses

Salvinia molesta is one of the world’s worst aquatic weeds and has cost Louisiana nearly $7 million worth of damage and economic impact each year. While aquatic herbicides and the biological control agent, Cyrtobagous salviniae, are the most efficacious control methods, there are limitations with each technology. Therefore, studies were conducted to evaluate insect mortality at upper and lower lethal temperatures and investigate integrated pest management (IPM) with insects and herbicides. Three Louisiana populations of C. salviniae were tested to evaluate cold tolerance and found that at 0°C, the Bayou Nicholas population was 1.3- and 1.4-times more cold tolerant …

Statistical And Technical Methodologies For Duplicated Multiple-Samples Preference And Attribute Intensity Sensory Ranking Test, Kennet Mariano Carabante Ordonez

LSU Doctoral Dissertations

Ranking tests are important preference and attribute difference tools for sensory evaluation. Replicated testing is used widely to reduce the number of panelists required in other sensory methods such as discrimination. The information regarding replications sensory ranking is limited. This research evaluated important statistical and technical aspects for the development of the foundation for duplicated sensory ranking tests. Three studies were accomplished: 1) A study of nonparametric analyses on real preference ranked data; 2) a sensitivity study of two samples serving protocols for duplicated visual ranking, and 3) protocols comparison in taste. In study 1, 125 panelists ranked in duplicates …

Impact Of Preemergent Herbicides, Mowing Height, And Fertilization On Centipedegrass Root Architecture And Drought Tolerance, Mike J. Adams

LSU Master's Theses

Mowing, N fertilization and the application of preemergence herbicides (PRE) are cultural practices performed on centipedegrass to improve overall turf quality. It is posited that increasing mowing height and application of fertilizers can lead to greater turfgrass rooting and increase drought survival; while the application of PRE is reported to potentially have negative impacts on rooting. At two locations in Louisiana, studies examining the effects of these practices on centipedegrass rooting and drought tolerance were conducted.

In the first 11-week study conducted, centipedegrass was treated with dithiopyr, pendimethalin, prodiamine, simazine, or indaziflam at the manufacturers’ labeled rates. During the 11-wk …

Modifiable And Non-Modifiable Factors Related To The Macular Pigment Optical Density (Mpod) In A Population Of College-Aged Adults, Genesis Gisselle Guerra Gaitan

LSU Master's Theses

Age-related macular degeneration (AMD) is one of the leading causes of blindness among the elderly worldwide. Retinal problems, specifically in the macula, hold potential for development of AMD. Macular pigment optical density (MPOD) gives a measure of the thickness of the macula and therefore health of the macula. Some of the populations at risk for development of AMD include being white, female, and having light eye color. Factors related to the development of the disease have been divided into modifiable and non-modifiable; non-modifiable include a genetic predisposition. Control of modifiable factors, including body mass index (BMI) and diet [dietary intake …

Impact Of Pre-Bloom Square Loss On Yield And Lint Quality In Louisiana Cotton Cropping Systems, Cory L. Cole

LSU Master's Theses

During the 2016 growing season, research was conducted at three locations in the state of Louisiana to evaluate the impact of pre-bloom square loss on cotton lint yield and fiber quality. Two cotton varieties, Phytogen 499 WRF and Phytogen 222 WRF were chosen up which to imitate early season square loss due to tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) and/or cotton fleahopper, Pseudatomoscelis seriatus (Reuter) feeding or unfavorable weather conditions. Thirty plants within each plot were selected and squares were counted. Squares were assigned numbers, and numbers were then randomized using a computerized number generator. To simulate intervals …

Optimization Of Overhead Irrigation In Relation To Irrigation Water Use Efficiency In The Louisiana Nursery Industry, Mark A. Wilson

LSU Master's Theses

Irrigation efficiency is an essential component of nursery production in the United States. To increase productivity of Louisiana growers, a series of studies were conducted to evaluate current nursery water practices. The objectives were to determine current irrigation practices; evaluate actual water usage; and compare nursery media for water utilization. A mailed survey determined that the majority of growers used can yard production (63%), overhead irrigation (79%), had no irrigation manager (82%) and did not collect/reuse water(61%); 50% do not test/calibrate their irrigation system. Based on this information, five nurseries using overhead irrigation were selected to determine water application and …

Isolation, Detection, And Prevention Of Foodborne Bacteria In Irrigation Water And Dairy Food, Mohammed Saleemallah Alhejaili

LSU Doctoral Dissertations

Foodborne microorganisms can be beneficial to humans or cause disease. Salmonella is one of the most causative foodborne disease outbreaks in the U.S.A. Salmonella has been detected in surface water used in irrigation, and can survive for weeks to years in water. The purpose of the study was to detect low levels of Salmonella spp. by comparing surfactant modified zeolite (SMZ) filtration and Activated Carbon (AC) filtration with the Environmental Protection Agency (EPA) method 1200. Also, to determine the generic E.coli counts and if there was a direct correlation of Salmonella to generic E. coli in irrigation water. The EPA …

Development And Evaluation Of Environmentally-Sensitive Genetic Male Sterile Lines For Louisiana Hybrid Rice, Federico Molina Casella

LSU Doctoral Dissertations

The Louisiana rice industry generates ~ 400 million dollars each year toward our state economy. Louisiana varieties are typically bred as pure lines, but hybrid (F₁) varieties have the potential to increase grain yields 15% or more over conventional sources. A primary goal of the LSU AgCenter Hybrid Breeding program is to breed elite cross combinations (hybrid varieties) through development of environmentally sensitive male sterile and fertile pollinator lines. Extensive multi-location yield trials in Louisiana have demonstrated high grain and head rice yields for candidate hybrids, but improvements in maturity, lodging, and grain chalk are needed. To address …

Louisiana Agriculture Fall, 2017, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Inheritance Study Of Resistance To The Herbicide Quizalofop-P-Ethyl In Rice And Development Of Provisia Hybrid Rice Germplasm, Jose Roberto Camacho Montero

LSU Doctoral Dissertations

In 2017, Provisia^TM quizalofop-p-ethyl (QFE) resistant rice became a new alternative for the chemical control of in-season red rice (Oryza sativa L. sp. spontaneum) infestations in Louisiana rice farms. Until then, Clearfield^® imidazolinone resistant rice was the only alternative that US farmers had for the efficient in-season chemical control of red rice. Provisia^TM rice is a BASF Corporation patented technology that can serve as an alternative for rotation with Clearfield^® rice. This strategy seeks to prevent outcrosses between red rice and herbicide resistant varieties, and consequently extend the life span of both technologies. This …

Effects Of Elicitor Induced Host Plant Resistance On Lepidopteran Insecticide Efficacy, Abigail Cox

LSU Master's Theses

Soybean looper (SBL), Chrysodeixis includens (Walker), is an important defoliating Lepidopteran pest of southern U.S. soybean and utilizes other agronomic crops and weeds as hosts. With increasing resistance to insecticides, alternative control strategies such as induced host plant resistance were evaluated against SBL. Jasmonic acid (JA) is an elicitor of host plant resistance, and was selected to determine its fit in an IPM plan for SBL. JA was applied to the top of meridic SBL diet and fed to SBL; no effects were found. JA applied as an exogenous elicitor to cotton, sweet potato, okra, cowpea, and soybean did result …

Rice Hull Bioreactor For Recirculating Aquaculture, Marlon A. Greensword

LSU Doctoral Dissertations

The engineering of floating media biofilters has been optimized over the years. The backwashing process has made them more energy and water efficient. Likewise, moving bed bioreactors (MBBR) are gaining interest and popularity because they are relatively affordable to build. Yet, developing countries’ aquaculture production remains largely excluded from the advances made in recirculating aquaculture systems (RAS). This discrepancy is partially driven by the high costs of media such plastic beads and Kaldnes (KMT) media, commonly used in MBBR.

This dissertation evaluates the usability and profitability of rice hulls (RH), an abundant by-product in many developing nations, as a sinking …

An Examination Of Agriculture Faculty Members' Involvement In And Perceptions Of Study Abroad Programs, Shelli Elizabeth Danjean

LSU Doctoral Dissertations

Globalization and internationalization are not new concepts. In the context of higher education, globalization can be viewed as the environment in which contemporary institutions function, while internationalization refers to the actions taken by universities and colleges in response to the changing environment. While internationalizing education has become popular rhetoric among institutions of higher education, is necessary to reflect on the actualization of internationalization goals in the closing of the decade. Can we hope to provide mechanisms for student engagement on an international level without continuing engagement of university faculty? As suggested by prior research, the answer is no. Faculty involvement …

A Preliminary Study To Evaluate Issues Involved Under In Vivo Conditions Of A Delivery System Containing Immobilized Lactobacillus Plantarum Ncimb 8826 On Rice Bran Fiber Extract, Chen Liu

LSU Master's Theses

Gut diseases like diarrhea, irritable bowel symptoms, and inflammatory bowel disease (IBD) can be treated with probiotics therapy. Probiotics have been recognized for their ability to promote the development of gut health and improve immune system. However, a difficulty involved with the use of probiotics is the need for and the ability to deliver a sufficient concentration of viable probiotic to the appropriate site. This can be difficult since the most common (and probably easiest) method of delivering the probiotic is orally. As such, the probiotic must be capable of surviving the hostile conditions of passage through the G.I. tract. …

A Comparison Of Soils And Their Associated Microbial Communities As Affected By Sugarcane Cultivation, Adam Francis Bigott

LSU Master's Theses

In Louisiana, sugarcane has been grown in the same soils for over 200 years. A phenomenon wherein soils with a long-term sugarcane cropping history produce decreased yields compared to adjacent land without a recent history of sugarcane cultivation has been documented in multiple sugarcane growing regions. Research in both Louisiana and internationally has shown positive plant growth responses when soils with a long-term cultivation history are sterilized or treated with selective biocides, suggesting there is a biological component to the underlying soil health issue. In this study, soil microbial ecology was compared for paired sites with short and long-term sugarcane …