Life Sciences Commons^™

Dsrna As A Potential Tool For Cercospora Leaf Blight Management And Elucidation Of A Factors That Contribute To Late Disease Onset In The Reproductive Growth Stage Of Soybean, Marija Zivanovic

LSU Doctoral Dissertations

Cercospora leaf blight (CLB) of soybean (Glycine max L. Merr.), an important disease in Louisiana, is primarily caused by Cercospora cf. flagellaris. The pathogen produces cercosporin, a toxin necessary for fungal virulence. So far, there are few commercial cultivars of soybean with CLB resistance and few effective fungicides to manage this disease. Consequently, alternative management approaches are needed. With that in mind, the first objective was to develop a screening method to compare the effectiveness of bacterially-expressed double stranded (ds) RNAs corresponding to genes associated with cercosporin accumulation (Avr4, CTB1, CTB8, AY-1 and HN-2 …

Eye-Hand Coordination Varies According To Changes In Cognitive-Motor Load And Eye Movements Used, Matthew Alan Yeomans

LSU Doctoral Dissertations

In this dissertation three studies were used to help improve the understanding of eye- hand coordination control of visuomotor reaching tasks with varying cognitive loads. Specifically, we considered potential performance differences based on eye-movements, postural influences, as well as fitness level of the young adult participants. A brief introduction in chapter 1 is followed by a detailed literature review in chapter 2. Results from the three studies presented in chapter’s 3-5 further advance our knowledge of the integrated control used for goal-directed visually-guided reaches. In the first study (chapter 3), the additional cost associated with the use of smooth pursuit …

Insights To Gray Seal (Halichoerus Grypus) Foraging Ecology From Stable Isotope And Dna Metabarcoding Analyses, Keith Michael Hernandez

LSU Doctoral Dissertations

The enactment of wide-ranging conservation laws in the United States enabled the recovery of many marine mammal species. However, as many species have surpassed predicted recovery goals, there is an increasing number of marine mammal-human interactions. For example, in the northeast US, the recovery of gray seals (Halichoerus grypus atlantica), coupled with declines in commercially important fishery species, has prompted discussions of revised management, and potentially lethal control measures. Much of this concern stems from seal-fisheries interactions, which necessitates an understanding of seal diets and foraging ecology. However, existing research is out of date and reliant primarily on …

Louisiana Agriculture Summer, 2020, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Sperm Decondensation And Male Pronuclear Formation In Bovine Intracytoplasmic Sperm Injection, Lauren Gatenby

LSU Master's Theses

This study assessed the effects both DTT and progesterone have on bovine spermatozoa to induce decondensation or the acrosome reaction in order to facilitate male pronuclear formation after ICSI. Sperm prepared by swim-up in a 5 mM concentration of DTT displayed time dependent morphological changes resulting in decondensation over a 6-hour period. An estimated 90% of treated sperm displayed early changes in morphology after the second hour of incubation. Sperm displaying partial decondensation of the nucleus was estimated as 42% by the 3^rd hour and increased to 62% by the 5^th hour of incubation. Fully decondensed sperm or …

Assessing Breastfeeding Knowledge, Attitudes And Intention Among Adult Female Undergraduate And Graduate Students, Kritee Niroula

LSU Master's Theses

This study measured the breastfeeding knowledge, attitude and intention of adult female students of Louisiana State University. Participants (n=1039; mean age 20.12 ± 3.122 years) were recruited to complete the questionnaire. Racial/ethnic representation was White (76.1%), African American (14.3%), Asian (5.5%), American Indian/ Alaska Native or Native Hawaiian/ Pacific Islander (0.8%) and Multi-racial (3.3%). The questionnaire was used to deliver a set of knowledge, attitude and intention items. Participants were evaluated based on their scores in each section. The questionnaire was pilot tested among five students for comprehensibility and understandability and necessary changes were made according to their suggestions. The …

Antimicrobial Strategies For Topical Applications, Kelsey Marie Lopez

LSU Doctoral Dissertations

Bacteria can thrive in diverse environments and are often harmless or beneficial. Bacteria in digestive tracts is one example of beneficial bacteria; however, bacteria can be harmful and when this type proliferates, it can cause infections within hosts. Bacterial infections are easily treated with antibiotics in most cases. However, bacteria are also capable of developing mutations which could cause them to become multi-drug resistant and eventually, “superbugs.” Therefore, the development of novel antimicrobial agents and materials capable of combating drug-resistant bacteria is necessary. Research presented in this dissertation focuses on different strategies for minimizing and preventing topical bacterial infections using …

Reconsidering The Obstetrical Dilemma: Correlations Between Head And Pelvic Size, Kelsey Catrice Fox

LSU Master's Theses

The Obstetrical Dilemma (OD) theory has become canon in biological anthropology. The OD posits that i] dystocia results from bipedal mothers and encephalized infants, ii] contrasting selection for bipedality and obstetrics hinders locomotive efficiency, and iii] the contradicting requirement of the fetus being small enough to pass through the birth canal yet being cognitively advanced enough to cling to its mother after birth. Females, theoretically, exhibit deficient gait efficiency for the sake of successful childbirth. An obstetric advantage theory has been posited where taller individuals with a larger head size have larger pelves. If the distance between the acetabulae increases …

Estimating The Onset And Extent Of Dieback Of Phragmites Australis Using The Normalized Difference Vegetation Index And Remotely Sensed Land Cover Classifications, Aimee M. Beaudette

LSU Master's Theses

Phragmites australis is cosmopolitan plant species with an invasive variety present throughout most of North America. In the Balize Delta, Louisiana, USA, P. australis plays an important role in combatting subsidence, maintaining navigation channels, and protecting interior fish and wildlife habitat from waves and storm surge. In 2016 a dieback of P. australis was reported by wetland managers, coinciding with the appearance of an invasive Asian scale insect (Nipponaclerda biwakoensis), though the specific cause is still unknown. Two previous efforts attempted to identify the onset of dieback conditions met with limited success. Using Landsat images from 1985 to …

Coastal Taxodium Distichum Growth Responses To Marine Influences And Stand Density, Stephanie Moothart

LSU Master's Theses

Ecological processes controlling transition areas of coastal wetlands between marshes (not forested) and swamps (forested) are poorly understood, despite important ecosystem services provided and strong interest in managing for ecosystem structure and function. The objective of this work was to identify how soil chemistry and stand density control individual-tree growth of the important coastal species baldcypress (Taxodium distichum (L.) Rich.) and pondcypress (Taxodium distichum var. imbricarium (Nutt.) Croom) along a coastal salinity gradient. Tree cores, soil samples, and stand density measurements were collected for sixty cypress trees at the coastal Joyce Wildlife Management Area, Louisiana, which is a …

Cover Cropping In Soybean-Corn Rotation System: Economic, Agronomic, And Soil Fertility Impact, Jose Rodolfo Mite

LSU Doctoral Dissertations

Planting winter-annual cover crops prevent soil erosion, reduces water runoff, and improves soil structure and soil quality. This research was conducted from 2017 to 2019 to evaluate the nutrient turnover of different species of cover crops in soils under different row crop production systems in Northeast and Central Louisiana. In Northeast Louisiana (Site 1, 2, and 3), treatments (cover crops and no cover crop) were arranged in a strip trial with three replications. At the Ben Hur Research Station, the treatments included three planting dates (September, October, and November) with [7 kg ha^-1 of phosphorus (P) and potassium (K)] …

Metabolic Network Analysis Of Filamentous Cyanobacteria, Daniel Alexis Norena-Caro

LSU Doctoral Dissertations

Cyanobacteria were the first organisms to use oxygenic photosynthesis, converting CO₂ into useful organic chemicals. However, the chemical industry has historically relied on fossil raw materials to produce organic precursors, which has contributed to global warming. Thus, cyanobacteria have emerged as sustainable stakeholders for biotechnological production. The filamentous cyanobacterium Anabaena sp. UTEX 2576 can metabolize multiple sources of Nitrogen and was studied as a platform for biotechnological production of high-value chemicals (i.e., pigments, antioxidants, vitamins and secondary metabolites). From a Chemical engineering perspective, the biomass generation in this organism was thoroughly studied by interpreting the cell as a microbial …

Insights Into Desiccation Tolerance: Properties Of Late Embryogenesis Abundant Proteins From Embryos Of Artemia Franciscana, Blase Matthew Leblanc

LSU Doctoral Dissertations

LEA proteins are a family of intrinsically disordered proteins that are expressed in various life stages of anhydrobiotic organisms and have been strongly associated with survival during water stress. The brine shrimp Artemia franciscana is the only known anhydrobiotic animal that expresses LEA proteins from Groups 1, 3, and 6. Here, I report that AfrLEA6, a novel Group 6 LEA protein, is most highly expressed in embryos during diapause and decreases throughout pre-emergence development. Notably, there is an acute drop in expression upon termination of the diapause state and the titer of AfrLEA6 during diapause is 10-fold lower than values …

Evidence Of Pathogen-Induced Immunogenetic Selection Across The Large Geographic Range Of A Wild Seabird, Hila Levy, Stephen R. Fiddaman, Julianna A. Vianna, Daly Noll, Gemma Clucas, Jasmine K. H. Sidhu, Michael J. Polito, Charles A. Bost, Richard A. Phillips, Sarah Crofts, Gary D. Miller, Pierre Pistorius, Francesco Bonnadonna, Celine Le Bohec, Andres Barbosa, Phil Trathan, Andrea Raya Rey, Laurent A. F. Frantz, Tom Hart, Adrian L. Smith

Faculty Publications

Over evolutionary time, pathogen challenge shapes the immunepheno type of the host to better respond to an incipient threat. The extent and direction of this selection pressure depend on the local pathogen composition, which is in turn determined by biotic and abiotic features of the environment. However, little is known about adaptation to local pathogen threats in wild animals. The Gentoo penguin (Pygoscelis papua) is a species complex that lends itself to the study of immune adaptation because of its circumpolar distribution over a large latitudinal range, with little or no admixture between ''different clades. Inthis study, we examine the …

Temporal And Spatial Trends Of Dead Copepods In The Northern Gulf Of Mexico In Relation To The Deepwater Horizon Oil Spill, Jessica L. Tolan

LSU Master's Theses

Zooplankton are a critical link between primary producers and higher trophic levels in the marine environment. They play a critical role in carbon cycling, are an important food source for commercially important species, and are excellent indicator genera for environmental changes that take place in marine ecosystems. This research quantified temporal and spatial patterns of dead copepods in response to the Deepwater Horizon Oil Spill (DWH) site using ZooSCAN imaging technologies. Research showed that the proportion of dead copepods (showed evidence of decay but no physical damage due to predation) increased with depth, and at time periods closest to the …

Exploring Effects Of Background Music In A Serious Game On Attention By Means Of Eeg Signals In Children, Fettah Kiran

LSU Master's Theses

Music and Serious Games are separately useful alternative therapy methods for helping people with a cognitive disorder, including Attention Deficit Hyperactivity Disorder (ADHD). The goal of this thesis is to explore the effect of background music on children with and without ADHD. In this study, a simple Tetris game is designed with Beethoven, Mozart music, and no-music. There are different brainwave techniques for recording; among others, the electroencephalography (EEG) allows for the most efficient use of BCI. We recorded the EEG brain signals of the regular and ADHD subjects who played the Tetris we designed according to our protocol that …

An Investigation Of Athletic Buoyancy In Adult Recreational And Sport Club Athletes, Jackie Rae Victoriano Calhoun

LSU Doctoral Dissertations

Athletic buoyancy is the ability of an athlete to effectively handle the daily setbacks and challenges they face during training and competition. Although buoyancy has received ample research in the academic domain, a dearth of information exists regarding buoyancy in the athletic domain. Therefore, the overall purpose of this dissertation was to investigate athletic buoyancy’s independent contribution to sport psychology while also exploring antecedents and outcomes in adult athletes.

Study 1 compared athletic buoyancy to other cognate constructs, or similar constructs, to determine their conceptual boundaries. A one-time, online questionnaire was distributed to 294 recreational athletes (M age = …

The Evolution Of Bivalve Shell Matrix Proteins, Mark Ira Duhon Ii

LSU Doctoral Dissertations

This dissertation focuses on the molecular underpinnings surrounding the evolution of the biomineralized shells of marine bivalves. Bivalve molluscs synthesize remarkably complex shells from calcium carbonate and an organic matrix of proteins secreted from the dorsal edge of the mantle. Molecular analyses of shell matrix proteins (SMPs) have suggested high rates of gene turnover despite the conserved nature of the shell itself. Here, I used proteomic and transcriptomic data to identify the SMPs and other biomineralization proteins from seven bivalve species that diverged 3-513 Mya. Contrary to previous studies that identified only a few shared biomineralization transcripts across the Bivalvia, …

The Effects Of Watermelon Juice Supplementation On Tissue Oxygen Saturation During Hyperglycemia, Sarah Elizabeth Kemp

Honors Capstones

No abstract provided.

The Nucleic Acid Binding Activity Of Human Mitochondrial Matrix Protein Miner2, Munira Khaled

Honors Capstones

No abstract provided.

Development Of High-Fiber Belgian Waffles And Evaluation Of Product Acceptance, Product-Elicited Emotion, And Purchase Intent By Millennial Consumers, 2253299685 Velasquez

LSU Master's Theses

Dietary fiber may help reduce LDL cholesterol levels and prevent cardiovascular diseases. The US-FDA recommends 14g of fiber/1000kcal consumed, but Americans consume/day. A product containing ≥5 grams of fiber/serving can be claimed as a high-fiber product. Adding dietary fiber to food affects their physicochemical properties and sensory quality, possibly leading to less product acceptability. This study was performed to assess selected physicochemical properties and consumer perception of “high-fiber” Belgian waffles. Waffles were formulated to have fiber contents of

Characterizing The Epidemiology Of Bluetongue And Epizootic Hemorrhagic Disease Virus In Louisiana, Michael Edward Becker

LSU Doctoral Dissertations

Bluetongue virus (BTV) and Epizootic Hemorrhagic Disease Virus (EHDV) are arthropod-borne viruses (Reoviridae; Orbivirus) transmitted by biting midges in the genus Culicoides. These viruses cause hemorrhagic disease and induce serious morbidity and mortality rates in wild and captive deer. Cattle are normally asymptomatic but serve as the reservoir/amplifying host for these viruses and play an important role in the transmission cycle. The objectives of this study were to characterize the epidemiology of these viruses as they relate to white-tailed deer and cattle, evaluate different trap types for collecting Culicoides midges, and determine if these viruses can be detected …

Assembly Of The Photosystem Ii Reaction Center, Annaliesa Renee Fanguy

Honors Capstones

No abstract provided.

Dna Methylation Analysis Of Ecr3 Of Oxytocin Receptor Gene, Amanda Husein

Honors Capstones

No abstract provided.

Feeding Ecology Of Red Snapper (Lutjanus Campechanus) And Vermilion Snapper (Rhomboplites Aurorubens) Coexisting At The Louisiana Shelf-Edge Banks, Katherine M. Ellis

LSU Master's Theses

Niche partitioning, the process by which competing species use different subsets of the available resources, is commonly used to explain the coexistence of closely related species. In the northwest Gulf of Mexico on the shelf-edge banks, red snapper (Lutjanus campechanus) and vermilion snapper (Rhomboplites aurorubens) are two coexisting closely related species. Yet, little is known about how these species partition resources. In this study, niche partitioning of red snapper and vermilion snapper was investigated using gut contents and stable isotopes. While dietary niche partitioning was apparent, the species relied upon similar prey and displayed isotopic niche overlap, …

Physical And Biological Factors Controlling The Fate Of Nitrate In A Louisiana Coastal Deltaic Floodplain, Alexandra Christensen

LSU Doctoral Dissertations

The Mississippi River Delta is threatened by a growing pressure to support large human populations in the United States both with food production, navigation systems, and urban development in the Mississippi River Basin. Nitrate-nitrogen load in the Mississippi River, up to 100 Tg N yr^-1 from agricultural and urban runoff, leads to phytoplankton blooms and hypoxia across the Louisiana continental shelf, creating dead zones of low dissolved oxygen threatening a significant commercial fishery. Along the coast and river corridors, floodplain ecosystems have the capacity to retain and remove nitrate. This dissertation explores the role of productive, actively growing coastal …

Gomcl: A T Gomcl: A Toolkit T Oolkit To Cluster O Cluster, Evaluate, And Extr Aluate, And Extract Non-R Act Non-Redundant Edundant Associations Of Gene Ontology-Based Functions, Guannan Wang, Dong-Ha Oh, Maheshi Dassanayake

Faculty Publications

Background Functional enrichment of genes and pathways based on Gene Ontology (GO) has been widely used to describe the results of various -omics analyses. GO terms statistically overrepresented within a set of a large number of genes are typically used to describe the main functional attributes of the gene set. However, these lists of overrepresented GO terms are often too large and contains redundant overlapping GO terms hindering informative functional interpretations. Results We developed GOMCL to reduce redundancy and summarize lists of GO terms effectively and informatively. This lightweight python toolkit efficiently identifies clusters within a list of GO terms …

Simulating Southern Rust Damage In Corn Through Defoliation, Michael Keith Shannon

LSU Master's Theses

The importance of leaf area to corn for grain production beginning at silking is well documented. However, being able to predict yield loss due to defoliators such as foliar plant diseases and insects that progressively increase in defoliation over time has been difficult to quantify. To address this issue, a leaf removal study was conducted at the Dean Lee Research and Extension Center located near Alexandria, Louisiana in 2017 and 2018. Two hybrids, differing in relative maturity were evaluated in this study. An early maturing hybrid (108 days in 2017 and 107 days in 2018) and a later maturing hybrid …

Louisiana Beef Cattle Marketing Strategies, Adam Barrilleaux

LSU Master's Theses

The purpose of this study was to estimate the financial costs and returns of selected marketing strategies for cattle producers in the state of Louisiana. Cattle for the project were from the Louisiana State University Agricultural Center and commercial producers. Cattle were grouped by weight and sex and vaccinated, castrated, and handled using standard best management practices. The standard marketing practice for weaned calves in Louisiana of selling weaned animals at local stockyards was compared against alternative marketing of preconditioned calves through video auctions or after feedlot finishing. Feedlot cattle were sold both on live weight value and carcass weight …

An Ecopath With Ecosim Analysis On Offshore Petroleum Platform Influences On Gulf Of Mexico Red Snapper, Valentin Gomez

LSU Master's Theses

Offshore oil and gas platforms have had a significant presence in the Gulf of Mexico since the 1950s. An important secondary function of these structures is that they provide artificial habitat to fisheries, most notably Red snapper. Policy changes intended to reduce the risk associated with aging infrastructure have reduced the number of standing platforms from 4044 to 1867 from 2001 to 2018. The effect this loss of habitat has on Red snapper was tested by creating three scenarios of platform changes and modeling the perturbation from 2005 to 2050. The simulation was accomplished using the ecological model Ecopath with …