Life Sciences Commons^™

Revealing The Native Status Of Conyza Bonariensis: Specialization Of Insect Herbivores Associated With Conyza And Erigeron Spp. (Asteraceae) In Louisiana, Texas, And Mississippi, Carlos Wiggins

LSU Master's Theses

Flaxleaf fleabane [Conyza bonariensis (L.) Cronquist] (Asterales: Asteraceae) is an annual herb which grows up to 1.0 m in height and is native to South America. Due to introduction and subsequent development of herbicide resistance, C. bonariensis has become a major agricultural weed in Australia. The purpose of this study was to catalog herbivorous insects associated with local populations of C. bonariensis in Louisiana, Texas and Mississippi in order to establish comparisons with the Australian fauna and identify potential biological control agents. Leaves, roots, stems, and flowers of C. bonariensis were inspected for signs of insects and pathogens. Results …

The Use Of Rna Interference To Investigate The Role Of Zmpr10 In Salt Stress Response And Mtl/Nld/Zmpla1 In The Improvement Of Haploid Induction In Maize, Max Carl Miller

LSU Master's Theses

RNA interference (RNAi) is a useful tool in gene characterization and its use is expanding into more practical fields. Two RNAi techniques were used in this case. The studies conducted in the chapter two use transgenic maize lines containing an RNAi construct targeting ZmPR10 to characterize its potential role in growth and development in response to salinity stress. T₁ transgenic plants from events A44S1-35-3 and A44S1-3-4, which had significantly reduced ZmPR10 expression compared to the non-transgenic controls, showed no differences compared to the controls in heights after 30 and 60 days, nor did the T₂ transgenic from these …

Evaluating Consumers’ Perception Of Gluten-Free/Clean-Label Muffins, Vanesa Chicaiza

LSU Master's Theses

The gluten-free-natural-ingredient (GF/NCL) product demand is globally increasing and driven by the growing prevalence of celiac-diseases. Nevertheless, the quality of GF product is often inferior compared to wheat-flour counterparts due to the removing of gluten, which, compromises the appearance, structure, and nutritional value. The addition of fiber into GF/NCL products has been considered to improve nutritional values while maintaining quality attributes. According to the US-FDA, a product containing ≥2.5 grams of fiber/serving can be claimed as a “good-source-of-fiber.” However, adding dietary fiber to baked products affects their qualities, and hence possibly lowering product acceptability.

The main objective of this research …

Effects Of Freshwater Input On Nutrient Loading, Toxic Cyanobacteria Bloom Development And Cyanotoxin Production In Lake Pontchartrain Estuary, Louisiana, Callie L. Snow

LSU Master's Theses

Estuaries in the northern Gulf of Mexico (GoM) are undergoing profound alterations due to changes in climate and implementation of coastal restoration activities and these may lead to the increasing frequency of harmful algal blooms (HABs). The introduction of large volumes of nutrient-rich, fresh Mississippi River water into nutrient-poor Lake Pontchartrain Estuary is known to substantially change the chemistry and ecology of the estuary, and warm temperatures paired with the persistent nutrient loading into the estuary supports the proliferation of potentially toxic cyanobacteria blooms (CyanoHABs). Throughout 2019, a long-term opening of the Bonnet Carré Spillway (BCS), initially from February to …

Hemp Microgreen Mineral Content, Cannabinoids, Total Phenolics, And Antioxidants, Cheston T. Schayot

LSU Master's Theses

Hemp (Cannabis sativa) L. microgreens were grown to 7 and 12 days with and without supplemental lighting and/or fertilizer. The principal macronutrient found in hemp microgreens was nitrogen (N), followed by potassium (K), phosphorus (P), magnesium (Mg), calcium (Ca), and sulfur (S). The principal micronutrient found in hemp microgreens was iron (Fe), followed by manganese (Mn), zinc (Zn), boron (B), and copper (Cu). Hemp microgreens were similar to other commonly grown microgreen species in minerals except for P and Mg, where they could potentially be an excellent source, and Zn, where they fell below previously reported amounts. The …

Response Of Roseau Cane (Phragmites Australis) To Two Biotic Stresses: Hyalopterus Pruni And Bipolaris Yamadae, Heather E. Cizek

LSU Master's Theses

Roseau cane (Phragmites australis) is considered an invasive plant because of its ability to replace native plant species. However, in Louisiana it plays an important role protecting coastal infrastructure and being part of the marsh ecosystem in the lower Mississippi River Delta. In recent years, Roseau cane has been affected by a die-off, a problem that has also been reported in some European countries. Possible biotic and abiotic factors that have been associated with the die-off include scale insects, climate change, pollution, salinity levels, and pathogens.

In this research, the individual and combined effect of a foliar disease …

3-Dimensional Structural Determination Of Epidermal Growth Factor-Like Repeat 27 From Mouse Notch1 Using Nuclear Magnetic Resonance Spectroscopy, Justin Grennell

LSU Doctoral Dissertations

NOTCH1 has been associated with a variety of human ailments, such as cancer and heart disease. NOTCH1 is a transmembrane protein that contains 36 epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding proteins on neighboring cells. Each repeat contains about 40 amino acids and has to be folded in a specific manner to function properly. The amino acid sequence, carbohydrate pattern, and calcium-binding sites of EGF-like repeats vary, but the pattern along the 36-repeat chain is conserved. The Abruptex region (EGF24-29) of NOTCH1 is a highly glycosylated (having undergone sugar addition) and flexible region, which gained its …

Improving The Deployment Of Insecticidal Seed Treatments In Louisiana Rice In Accordance With Integrated Pest Management, Megan Marie Mulcahy

LSU Doctoral Dissertations

Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is the most destructive insect pest of rice in the United States. Lissorhoptrus oryzophilus larvae feed on rice roots, reducing yields by up to 25 %. Lepidopteran stem borers, in the family Crambidae, are also economically important pests of rice in Louisiana.

Currently, insecticidal seed treatments are used throughout the U.S. Mid-south rice industry to prophylactically control L. oryzophilus. Chlorantraniliprole seed treatments are also effective against stem borers. Thus, insecticidal seed treatments are used on >80 % of Louisiana rice acreage. Although seed treatments reduce damage inflicted by these pests, they are applied preventatively …

Investigation Of Bovine Herpesvirus-1 Determinants Of Neuronal Infectivity In Novel Bovine Neuronal Cell Systems For Development Of A Safer Viral Vaccine Vector, Jared Rudd

LSU Doctoral Dissertations

Bovine herpesvirus-1 (BoHV-1) is the causative agent of infectious bovine rhinotracheitis and a component of the bovine respiratory disease complex. BoHV-1 is a significant burden on the cattle industry in the United States causing severe respiratory disease, abortions, loss of calves (“shipping fever”). BoHV-1 belongs to the alphaherpesvirus subfamily of Herpesviridae. Alphaherpesviruses enter axons of sensory nerves and establish life-long latency in trigeminal and dorsal root ganglionic neurons after primary infection of the oronasal or genital epithelium respectively. Modified live attenuated vaccines for BoHV-1 are commercially available but are limited in their protection while retaining the ability to establish latency …

Responses Of Wetland Soil Microbial Communities To Different Types Of Anthropogenic Stress, Grace Ann Cagle

LSU Doctoral Dissertations

Microorganisms are the major drivers of biogeochemical transformations of carbon and nitrogen in wetlands. These processes are crucial for ecological sustainability and are heavily impacted by human activities. How anthropogenic stress affects the microbial drivers of carbon storage and nutrient cycling in wetlands is not well understood. I investigated the response of wetland soil microbial communities to three different types of anthropogenic stress using DNA sequencing. First, I studied the effect of warming on microbial carbon processing in a peatland underlaid by permafrost by comparing the abundances of specific genes between sites subjected to five years of warming and controls. …

Regional Expansion And Evaluation Of Potential Chemical Control For Invasive Apple Snails (Pomacea Maculata) In Southwest Louisiana, Julian M. Lucero

LSU Master's Theses

The integration of monitoring and chemical control is an efficient strategy for managing invasive apple snails, Pomacea maculata, in the rice (Oryza sativa L.) and crawfish systems of southwest Louisiana. However, their current distribution, expansion rates, and susceptibility to chemical control methods in this area are not well known. This study evaluated the expansion of P. maculata in southwest Louisiana and assessed potential chemical control for P. maculata among toxicity assays using various application rates. The effects of potential chemical control were also assessed on a non-target species, the red swamp crawfish (Procambarus clarkii). P. maculata …

Brood Abundance And Invertebrate Availability In Crop-Dominated Landscapes In The Prairie Pothole Region, Catrina V. Terry

LSU Master's Theses

The Prairie Pothole Region (PPR) is the most important region for ducks in North America, producing over half the ducks on the continent, and it is a priority landscape for wetland and grassland conservation. Agricultural expansion has changed the PPR, and the majority of grasslands and potholes have been converted into row-crops. The loss of nesting habitat has directly caused nest success to decline. Most of the remaining wetlands are surrounded by row-crops, and are considered lower quality because they receive runoff of sediment and chemicals, which may decrease primary forage for young ducklings as well as inhibit wetland vegetation. …

Fish Gelatin Coating As An Antimicrobial Agent On Wild-Caught Louisiana Shrimp, Hunter S. Songy

LSU Master's Theses

Seafood has a short shelf-life because of its unstable nature. Shrimp has a shelf-life of only eight days. Edible coatings, like those made from gelatin, have been used to apply antimicrobial agents to food product surfaces. Organic acids are naturally occurring, low-cost options that have been proven to effectively reduce bacterial loads on food products and extend their shelf-life. Gelatin can be extracted from catfish skin, helping to reduce product waste. The purpose of this study was to determine the potential effectiveness of fish skin gelatin as an antimicrobial coating alone and in combination with lactic acid, and then compare …

The Predictive Influence Of Challenging Behavior On Parent Stress In Young Children With Autism Spectrum Disorder, Paige Weir

LSU Master's Theses

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by deficits in social communication, restricted interest, and repetitive patterns of behavior. Individuals with ASD also exhibit challenging behaviors that affect parent and caregiver stress directly. However, researchers have not yet examined the predictive influence of specific challenging behaviors on parent stress, particularly in young children (i.e., infants and toddlers) with ASD. Therefore, the current study expands existing literature by a) investigating the influence that challenging behaviors of young children with ASD have on parent stress and b) examining the unique contribution that each behavior (i.e., aggressive/disruptive behavior, stereotypy, and self-injurious …

Willingness Of Non-Industrial Private Forest Landowners To Obtain A Private Versus Public Consultation, Erika Largacha

LSU Master's Theses

Promoting conservation practices among family forests landowners play a critical role to enhance forest health and ecosystem services. Several certification programs recognize such efforts, but participation among landowners varies based on their characteristics, program requirements, and incentives. One crucial step of certification is to obtain a forest management plan, but acquisition among landowners remains extremely low. Several studies suggest connecting landowners with foresters as the key element towards the adoption of forest management plans. Because foresters are typically employed by public forestry agencies or as private self-employed consultants, landowners' willingness to work with either may differ. As far as we …

Investigation Of Arabidopsis Extremophyte Relatives, Schrenkiella Parvula And Eutrema Salsugineum Reveals Different Roads Leading To Salt Stress Tolerance, Kieu-Nga Thi Tran

LSU Doctoral Dissertations

How plants adapt to salt stress has been a central question in plant biology for decades. Yet we have not been able to fully understand the molecular networks and genetic mechanisms underlying this complex trait. Most of the genetic work on salinity stress has focused on understanding salt stress responses in the leading, yet a salt-sensitive model Arabidopsis thaliana. With the recent availability of genomes for wild-relatives of A. thaliana, we can now investigate how naturally salt adapted plants may have evolved modified or novel molecular networks to adapt to salt stress. Therefore, my research utilizes a comparative …

Evaluation Of The Effectiveness Of Aeration During Washing To Dislodge Microorganisms From Cucumbers And Bell Peppers, Julysa Benitez

LSU Master's Theses

Microorganisms may attach firmly to produce matrices and could affect the efficacy of disinfectants. The purpose of this study is to evaluate the effectiveness of aeration to reduce microbial risk during washing and sanitizing of fresh produce. Bell peppers and cucumbers were spot inoculated with Listeria innocua, E. coli O157:H7, and Salmonella; after drying in the biosafety cabinet for 1h, these were washed with water or sanitized with 100 ppm of chlorine for 3 min with or without aeration. Bell pepper and cucumber samples were placed in sterile bags with PBS after treatment. Bell peppers were processed in the stomacher …

Corn Nitrogen Management Under Different Tillage Systems, Akshara Athelly

LSU Master's Theses

Adoption of no-tillage (NT) and cover crops (CC) significantly effect soil physicochemical properties and nutrient cycling that necessitates modified nutrient management to maximize crop yields. Two field experiments were conducted to evaluate N-sources Urea Ammonium Nitrate (UAN), urea, and urea+ N-stabilizer (urea+ stab) and N-split applications 100%N at V2 (S1), 25%N at V2+75%N at V6 (S2), 25%N at V2+ 50%N at V6+ 25%N at VT (S3) in a sandy loam soil and three CC wheat (Triticum aestivum), hairy vetch (Vicia villosa) , and radish (Raphanus sativus) and their combinations along with three N-rates ( …

Insights Into Abscisic Acid And Phosphatidic Acid Signal Transduction Dynamics In Arabidopsis Thaliana, Ruth Wanjiru Ndathe

LSU Doctoral Dissertations

Abscisic acid (ABA) is a plant hormone that triggers a signaling transduction in response to drought, salinity, and cold. The core components in the ABA signaling pathway that lead to downstream ABA responses have been identified.

Phosphatidic acid (PA) is a membrane phospholipid that is involved in the signaling pathway for ABA-induced stomatal closure. We tested whether PA is involved in the signaling pathway for ABA-induced gene expression as well, using model plant Arabidopsis thaliana. We selected the RD29A gene which is a canonical drought gene that is induced by ABA. We found that PA is not involved in …

Genetic Structuring And Community Assembly In Neotropical Birds, Oscar Johnson

LSU Doctoral Dissertations

The factors that influence how species form is of fundamental interest to biology. Under the framework of the speciation cycle, the process begins by population subdivision creating structured populations that then diverge until two new species form. Following speciation, however, continued divergence is necessary for these new species to be able to coexist without adverse interactions, known as the transition to secondary sympatry. At this point the speciation cycle is complete and the process can begin anew. Many of the factors influencing these processes remain poorly understood. Here, I explore the factors that influence speciation and community assembly in Neotropical …

Using Uav Technology Paired With Multispectral Cameras To Assess Crown Rust Epidemics In Oats, Turner A. Graham

LSU Master's Theses

Crown rust, caused by Puccinia cornonata f. sp. avenae, is a common disease of oats (Avena sativa) found virtually everywhere oats are cultivated. This disease has caused yield losses of 10 to 40% worldwide. Early detection is important for effective management. A more recently utilized technology in agriculture is unmanned aerial vehicles (UAVs). UAVs, or drones, equipped with cameras are now being used as a resource to take images of fields to identify pests and other issues that may be occurring. Normalized differentiated vegetative index (NDVI) is a numerical indicator used to determine the vegetative health of …

Evaluating Substrate Stratification Practices For Enhanced Water Resource Management In Nursery Production, Kristopher S. Criscione

LSU Master's Theses

The nursery and greenhouse industries have continued to increase in production and sales over the past two decades. This increase in horticultural production necessitates a greater demand for finite natural resources, specifically water. Sustainable water resource management will be critical to the success and continual growth of the nursery industry. Strategic irrigation scheduling and innovative substrate management practices can improve nursery resource efficiency. Soilless substrates were originally utilized by the industry to increase substrate air-filled porosity, to ensure adequately drained container systems. Thus, the implementation of these soilless substrates can lead to an inefficient use of water and fertilizer. Substrate …

Casting A Critical Lens On Global Development: A Multimethod Investigation Of The Masculine Hegemonic Forces That Marginalize Women In Agriculture, Morgan Alyse Richardson Gilley

LSU Doctoral Dissertations

A critical link between gender equality and agricultural development has been established in the literature. However, disparities between genders have persisted in many areas of the developing world. One strategy that has been used to reduce gender inequalities has been educational outreach opportunities through agricultural extension, which has provided women a critical pathway to empowerment. Despite this, a lack of knowledge on the experiences of women in agriculture throughout Southeast Asia has served as a barrier to the advancement of such work. This investigation sought to better understand the issues facing this region’s fight to attain gender equality. To achieve …

Consumers' Perception Of Food Containing Insect Protein Affected By Serving Condition And Questionnaire Protocol, Yupeng Gao

LSU Master's Theses

Edible insects are a new sustainable protein source compared to meat. Western consumers show aversion to the prospect of insects as food due to unfamiliarity, food neophobia, and negative emotions. This dissertation research explored consumers’ emotional perception of insect-based food products presented under different testing conditions and examined the influence of other people’s emotional expressions towards consumers’ purchase decisions. To identify and rate emotion intensities, tests were conducted using three presenting formats including text, image, and an actual product; all of which were applied for both cookies containing insects and without insects. In addition to these presenting formats, news of …

High Precision Tissue Sampling For Mass Spectrometry, Chao Dong

LSU Doctoral Dissertations

A high precision infrared laser ablation sampling system was developed for localized tissue proteomics. A Schwarzschild reflective objective with 10 cm working distance and 0.3 numerical aperture was used to focus a mid-IR laser at 3 µm wavelength to a beam diameter of approximately 6 µm. A laser ablation spot size of 10 µm was achieved on thin ink films and tissue sections. The sampling system was used to ablate and capture rat brain tissue sections in transmission geometry. Captured tissue protein was extracted, digested, and analyzed with offline liquid chromatography tandem mass spectrometry (LC-MS/MS) for peptide and protein identification. …

Ontogeny And Resource Use Of Invasive Lionfish, Pterois Volitans, In Shallow Water Habitats Surrounding Summerland Key, Emily Shallow

LSU Master's Theses

The invasive red lionfish, Pterois volitans, has been shown to considerably reduce reef biomass via predation and competition in non-native ranges. Primary maintenance of lionfish is through human removal (e.g., culling), which does not sufficiently control the population due to deep-water spawning, recolonization of recently culled reefs, and ontogenetic migrations to mesophotic waters. Here, I investigated the diets and primary energy pathways during ontogeny in lionfish from the Florida Keys using stomach contents and stable isotope (δ¹³C and δ¹⁵N) analysis to better understand their role in the trophic food web. Additionally, isotopic overlap between lionfish …

How Object Storage Skills Relate To Language Development, Jessica Laurent

Honors Capstones

No abstract provided.

Parent Play Correlates With Infants’ Object Construction Skill, Makayla Barber

Honors Capstones

No abstract provided.

Comparing Expression And Localization Of Syntaxin-1a And The Cystic Fibrosis Transmembrane Regulator In Adult Gallus Gallus Retinas, Rachel Lauren Butz

Honors Capstones

No abstract provided.

Exploration Of Staphylococcus Aureus Immune Inhibitory Proteins’ Structures And Investigation Of Their Effects On Monocyte And T Cell Adhesion, Jena Elizabeth Bordelon

Honors Capstones

No abstract provided.