Life Sciences Commons^™

The Development Of Diving Capabilities In Weddell Seal (Leptonychotes Weddellii) Pups Throughout Early Ontogeny, Emma Weitzner

Master's Theses

Weddell seals (Leptonychotes weddellii) are among the deepest diving pinnipeds (i.e., seals, sea lions, and walrus) and one of the best studied marine mammals in the world; as such, these seals are considered a model species for the study of diving physiology and behavior. Adult Weddell seal dive physiology is rather comprehensively understood, yet previous research has excluded an examination of pups’ initial independent diving attempts, beginning instead with the diving capabilities of near-weaning individuals at four to five weeks of age. This is beyond the point many pups have attempted their first independent dives; pups begin to …

An Analysis Of Changes In Stream Temperature Due To Forest Harvest Practices Using Dhsvm-Rbm, Julia B. Ridgeway

Master's Theses

Forest harvesting has been shown to cause various changes in water quantity and water quality parameters, highlighting the need for comprehensive forest practice rules. Studies show a myriad of impacts to ecosystems as a result of watershed level changes, such as forest harvesting. Being able to better understand the impact that forest harvesting can have on stream temperature is especially critical in locations where federally threatened or endangered fish species are located. The overall goal of this research project is to assess responses in stream temperature to various riparian and forest harvest treatments in a maritime, mountainous environment. The results …

Compact Farms: A Step Towards Sustainability, Chad A. Porter

Agricultural Education: Graduate Internship Reports

This project report accounts for the potential benefits compact farms can have for agriculture and sustainability. The report details the design, implementation, and management of Whistle Stop Farm, a 1-acre, diverse farm producing vegetables, fruits, and gourmet mushrooms for direct markets in Atascadero, California. The farm focuses on economic and environmental sustainability. A large part of the farm revolves around soil science and how to manage soils to promote a healthy soil ecosystem.

Graduate Internship Report - Watsonville High School, Savanah D. Rhine

Agricultural Education: Graduate Internship Reports

This internship and project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Dinuba High School conducted by representatives of the California Department of Education. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Watsonville High School, the agriculture program and the community.

Aged 539: Internship In Agriculture Education, Veronica Lemus

Agricultural Education: Graduate Internship Reports

This internship and project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Fillmore High School conducted by representatives of the California Department of Education. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Fillmore High School, the agriculture program and the community.

Graduate Internship Report- Orestimba High School, Briana Vincent

Agricultural Education: Graduate Internship Reports

This Graduate Internship report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are currently used for the Agriculture Incentive Grant review process at Orestimba High School.The internship project includes the improvement of the recruitment for Orestimba High School. The included information and supporting materials are required to receive local and state funding. An overview of the Orestimba High School Program can be found in the Comprehensive Program Plan that is included in this document.

Availability Of Microtrash Materials And Selectivity By California Condors, Marie G. Solis

Biological Sciences

California condors consume non-food items, including anthropogenic waste, and provision it to their young. Consuming “microtrash” can cause mortality, especially in nestlings. There are several hypotheses explaining why condors might consume microtrash. These hypotheses assume condors exhibit a preference for the type of material. Therefore, we test the assumption that when California condors take microtrash, they display material selectivity. We did this by sampling microtrash from roadside pullouts in the Los Padres National Forest. Those samples were compared to condor necropsy and nest microtrash data, categorized by material type: glass, plastic, metal, and other. We find that in our analysis …

Effects Of Barotrauma On Four Species Of Pacific Rockfishes (Sebastes Spp.), Lesley Salter

Master's Theses

Physoclistic fish, such as Pacific rockfishes (genus Sebastes), have closed swim bladders that help them regulate their buoyancy. When anglers catch these fish and reel them to the surface, gases within their swim bladder expand due to the decrease in pressure. This can cause their swim bladder to over inflate––a condition known as barotrauma. Overly buoyant fish experiencing barotrauma often struggle to swim back to dwelling depth if released at the ocean’s surface. These fish may experience high rates of mortality by thermal shock caused by the warmer surface temperatures, starvation, predation, or vision problems caused by barotrauma. Assisted …

Study Of Paper Microbial Fuel Cells For Use In On-Site Wastewater Testing, William A. Tolmasoff

Master's Theses

This study demonstrated a technique for fabricating simple, low-cost Paper Microbial fuel cells (PMFC’s) in the model of a previous study to, for the first time, produce voltage from wastewater effluent. The PMFC’s were created by stacking and gluing the main components of an MFC together: reservoir layer; anode; cation exchange membrane (CEM); air cathode. A wax printer was used to create the hydrophobic borders of the PMFC’s on filter paper, and graphite paint was applied to the paper to create the anode. The CEM’s considered were filter paper, wax, and Nafion, with Nafion being the most efficient. Finally, the …

The Effects Of Agricultural Waste-Based Compost Amendments In Organic Pest Management, Gregg T. Stephenson

Master's Theses

Soil-borne pests and pathogens, such as Verticillium dahliae, can devastate a wide range of annual and perennial crops. Current management options for organic production are limited and sustainable management of pathogens, weeds, and arthropods is important for staying profitable and reducing the use of harmful chemicals. Organic soil amendments play an important role in supplying some of the nutritional needs of vegetable crops and improving soil structure, while also contributing to pest control. The objective of this research was to determine the effects of grape and olive-based composts on soil pathogen load, arthropod communities, and weed biomass and diversity. …

Carbonate Chemistry Characterization In A Low-Inflow Estuary With Recent Seagrass Loss, Jolie Higgins

Master's Theses

Estuaries are dynamic environments that are strongly affected by natural variability, as well as direct and indirect anthropogenic impacts. A better understanding of the drivers of carbon fluxes and biogeochemical variability in estuarine systems is needed, particularly with the increasing threat of ocean acidification. Morro Bay in Central California is a small nationally protected estuary, with seasonally low freshwater inputs. Since 2007, the bay has experienced a significant loss of native seagrass, Zostera marina, which is an important component of the marine ecosystem. Because seagrass photosynthesis decreases carbon dioxide and increases oxygen in the water column, the loss of seagrass …

Strawberries And Gut Health In Postmenopausal Women, Morgan T. Macneill

Master's Theses

The gut microbiota has been implicated in both health and disease. As such, diet is a significant determinant of gut health, whereby diet induced dysbiosis is associated with cardiometabolic risk. Interestingly, a higher proportion of Firmicutes and a lower proportion of Bacteroidetes are implicated in obesity. Strawberry polyphenols have been shown to reduce cardiovascular disease risk in addition to exhibiting prebiotic activity by increasing probiotic bacteria in the gut. Polyphenols have also been shown to reduce the ratio of Firmicutes to Bacteroidetes. Therefore, dietary modifications such as strawberry consumption may help improve health outcomes through the gut. The objective of …

Saponin Removal From Quinoa By Abrasion Processing, Luke Lundberg

Master's Theses

Quinoa is coated with a thin layer of saponins, glycosylated triterpenoids, that produce a bitter flavor when consumed. The average saponin content in commercial varietals from Bolivia average around 2.7% saponins and organoleptic testing shows the threshold for noticing a bitter flavor is below 0.12% (Medina-Meza et al., 2016). Current industrial processing methods use a combination of abrasion and turbulent water flow to remove saponin. This study will address the following research question: How will grain-to-grain and grain-to-surface abrasion affect the processing time to remove saponin from quinoa? In particular, can effective saponin removal be achieved in less than 10 …

Dna And Protein Sequence Analysis Of Neuronal Markers Neuronal Nuclei (Neun) And Doublecortin (Dcx) In The Northern Pacific Rattlesnake (Crotalus Oreganus) And Western Fence Lizard (Sceloporus Occidentalis)., Brett M. Vassar

Master's Theses

Neuronal Nuclei (NeuN) and Doublecortin (DCX) are neuron specific proteins that are used in histological studies of brain structure in a variety of vertebrate taxa.Antibodies against NeuN (anti-NeuN) bind to the Fox-3 protein, an RNA binding protein common in mature neurons. Anti-DCX labels a microtubule-associated protein expressed in actively dividing neural progenitor cells and migrating neurons. The DCX gene encodes a protein that is well conserved across mammalian, avian, and a few reptilian species, therefore anti-DCX staining has been used successfully across a range of vertebrate taxa. Successful neuronal staining using anti-NeuN has been demonstrated in mammals, birds, and the …

The Proteomic Response Of Northern Elephant Seal (Mirounga Angustirostris) Pups To Physiological Stress During Development, Melissa P. Voisinet

Master's Theses

Background: Northern elephant seals transition from terrestrial nursing pups to pelagic foraging juveniles in a short period of just 8-12 weeks. During the post-weaning period, pups rely solely on the energy reserves gained during nursing for their caloric demands and water supply. The prolonged absence of food after weaning is the first of many fasts for which the seals have evolved adaptations such as decreased urine production and increased blubber reserves. The stressors experienced from learning to dive for the first time are also stressors that they will experience frequently as an adult and for which they have evolved adaptations. …

Exploring Correlates Of Infant Clarity Of Cues During Early Feeding Interactions, Alison K. Ventura, Sierra Sheeper, Jordyn Levy

Kinesiology and Public Health

Background Recommendations aimed at reducing infants’ risk for rapid weight gain primarily focus on promoting caregivers’ use of responsive feeding practices and styles. These recommendations are grounded in the belief that infants will effectively signal hunger and satiation to their caregivers. To date, few studies have explored how variability in infants’ communication of hunger and satiation may contribute to feeding interactions.

Objective Our aim was to explore variability in, and correlates of, infant clarity of cues during feeding interactions.

Design This was a cross-sectional study.

Participants/setting Mother-infant dyads (n = 86) were video-recorded during a typical feeding interaction within laboratory-based …

Graduate Internship Report- Dinuba High School, Nicole R. Borba

Agricultural Education: Graduate Internship Reports

This graduate internship report includes the documentation required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Dinuba High School conducted by representatives of the California Department of Education. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Dinuba High School, the agriculture program and the community.

Graduate Internship Report - Bakersfield Christian High School, Jennifer Michelle Downs

Agricultural Education: Graduate Internship Reports

This internship and project report includes documentation required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Bakersfield Christian High School conducted by representatives of the California Department of Education. The supporting material includes information to receive charter status as a private high school, outline the goals and objectives of the program, along with an overview of Bakersfield Christian High School, the agriculture program and the community.

Comparative Thermal Ecology Of Coastal And Inland Pacific Rattlesnakes (Crotalus Oreganus), Hayley Layne Crowell

Master's Theses

Global biodiversity is declining as a direct result of anthropogenic climate change. Ectothermic species have become focal organisms for studying the ecological effects of altered climates due to the clear relationship between environmental temperatures and ectotherms’ basic physiological functions. Historically, examinations of these effects have focused heavily on heliothermic lizards, and most others have tended to focus on single populations or sympatric species within a single community. Addressing the longterm energetic implications of environmental temperature variation will provide valuable insight into the cascading physiological effects that certain populations or species may experience as a result of altered climates.

In this …

Graduate Internship Report - Highland High School, Meagan Dunlap

Agricultural Education: Graduate Internship Reports

This internship and project report includes documentation required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Highland High School conducted by representatives of the California Department of Education. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Highland High School, the agriculture program and the community.

Graduate Internship Report - Fowler High School, Victor Evans

Agricultural Education: Graduate Internship Reports

This internship/project report includes validation documents required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Fowler High School conducted by representatives of the California Department of Education. The internship included the construction of a facility to house and care for fair livestock. Accompanying the project report are photos illustrating the construction steps and educational processes utilized to complete the project.

Graduate Internship Report - Foothill High School, Bakersfield, Donald Vernon Clark

Agricultural Education: Graduate Internship Reports

This document focuses on addressing the 11 quality criterias of the Bakersfield Foothill agriculture department. Current status, ideas for imporovment, and areas to improve on are addressed in each criteria. The supporting documents are concurrently used for the Agriculture Incentive Grant review process at Foothill High School. After the completion of this project the Bakersfield Foothill Agriculture Department is in compliance with the quality criteria of the Agriculture Incentive Grant expectations.

Effects Of Varying Doses Of Oral Bisphenol A Consumption On Type 2 Diabetes Risk Markers In Healthy Adults, Todd Hagobian, Alyssa Bird, Sean Stanelle, Dana Williams, Andrew Schaffner, Suzanne Phelan

Kinesiology and Public Health

Objective

To determine the effects of varying doses of orally administered BPA on indices of glucose metabolism.

Methods

Eleven college students (21.0 ± 0.8 years; 24.2 ± 3.9 kg/m²) were randomized in a double-blinded, crossover fashion separated by >1 week to placebo (PL), deuterated BPA at 4 µg/kg body weight (BPA-4), and deuterated BPA at 50 µg/kg body weight (BPA-50). Total BPA, glucose, insulin, and C-peptide were assessed at baseline, minutes 15, 30, 45, 60, and every 30 minutes for 2 hours in response to a glucose tolerance test.

Results

There was a significant condition × time interaction …

The Effect Of Alcohol And Bitterness Levels On Brewing Yeast Viability, Nichole Elizabeth Bryant

Master's Theses

Two of the most popular beer styles within the craft brewing industry are India Pale Ales (IPA’s) and those with high alcohol by volume (ABV). Production of these styles requires high gravity fermentation and high amounts of bittering hops in order to reach the required values for ABV and International Bitterness units (IBU) respectively. The aim of this study was to determine the impact of high gravity fermentation and high IBU levels on yeast viability and repitching cycles.

An initial experiment on high gravity fermentations was done in order to assess the effects this variable had alone. Successive five day …

Effectiveness Of Windrow Composting Methodology In Killing A Thermo-Tolerant Species Of Salmonella During Mortality Composting, Spencer Gabriel Myers

Master's Theses

In a large agricultural operation, such as the one at Cal Poly San Luis Obispo, disposal of deceased animals is an immense issue. The cost of transporting and rendering every dead animal is inhibitory to the general function of the agricultural operations and their thin budget. Therefore, we propose that composting mortalities could be an economical alternative. Composting is a recognized method for taking animal waste products along with carbon waste and turning it into a pathogen-free, nutrient-rich topsoil. Carcass composting is in fact performed in other countries and states to varying degrees of success. However, the California EPA limits …

Fabrication Of Miniaturized Paper-Based Microfluidic Devices (Micropads), E. Brandon Strong, Spencer A. Schultz, Andres Martinez, Nathaniel W. Martinez

Chemistry and Biochemistry

Microfluidic paper-based analytical devices (microPADs) are emerging as cost-effective and portable platforms for point-of-care assays. A fundamental limitation of microPAD fabrication is the imprecise nature of most methods for patterning paper. The present work demonstrates that paper patterned via wax printing can be miniaturized by treating it with periodate to produce higher-resolution, high-fidelity microPADs. The optimal miniaturization parameters were determined by immersing microPADs in various concentrations of aqueous sodium periodate (NaIO₄) for varying lengths of time. This treatment miniaturized microPADs by up to 80% in surface area, depending on the concentration of periodate and length of the reaction …

An Albino Mutant In Brassica Rapa Maps To The Pdx2 Locus On Chromosome 10, Beatriz M. Garcia

STAR Program Research Presentations

We have identified an albino mutant in the plant Brassica rapa. This recessive mutation could provide insights into the photosynthetic pathways of all plants. Previously the mutation was mapped to chromosome 10 of Brassica rapa. To narrow down the chromosomal region containing the albino mutation, we grew a segregating population of plants. DNA was extracted from albino and wildtype (green) plants and genotyped using PCR markers on chromosome 10. The genotype detected using the INDEL2 marker perfectly predicted whether a plant was albino or WT. This indicates that the INDEL2 marker is tightly linked to the albino mutation. INDEL2 is …

An Albino Mutant In Brassica Rapa Maps To The Pdx2 Locus On Chromosome 10, Cezar L. Sanchez, Beatriz M. Garcia, Edward Himelblau, Nick Diaz

STAR Program Research Presentations

We have identified an albino mutant in the plant Brassica rapa. This recessive mutation could provide insights into the photosynthetic pathways of all plants. Previously the mutation was mapped to chromosome 10 of Brassica rapa. To narrow down the chromosomal region containing the albino mutation, we grew a segregating population of plants. DNA was extracted from albino and wildtype (green) plants and genotyped using PCR markers on chromosome 10. The genotype detected using the INDEL2 marker perfectly predicted whether a plant was albino or WT. This indicates that the INDEL2 marker is tightly linked to the albino mutation. INDEL2 is …

Cichlid Size Vs Flow Rate, Amy E. Naumovski

STAR Program Research Presentations

Cichlids are a diverse group of fish that are known for their prenatal and postnatal care of their eggs and frys (baby fish). This unique characteristic creates a limitation on the amount of eggs a cichlid can lay and protect, which is particulalry effected by the size of the eggs. Cichlids have a large variance in their egg size, which correlates to a large variance in their fry size. This project explores the trade offs cichlids make in egg size and fry size by specifically testing the ability of varying sized cichlids to swim in different flow rates. This could …

Comparing Orientation Behavior And Prey Preference Of Leptasterias Spp. Between Microhabitats, Jenna C. Sanders, Margaret W. Johnson, C Sarah Cohen

STAR Program Research Presentations

Leptasterias spp. is a species complex of small, six rayed, predatory sea stars that inhabit rocky intertidal communities along the Pacific coast of North America. A developmental mode of brooding their young, rather than broadcasting for planktonic development, limits dispersal away from the natal area, and may also result in fine scale local adaptation of populations. Local adaptation may lead to morphological and behavioral differences among populations, specifically towards the most available prey. Within the rocky intertidalthere are zones of high and low wave impact which create microhabitats with their own selective forces. In areas of high wave impact, stars …