Life Sciences Commons^™

Vineyard Age Effect On Juice Chemistry, Anthocyanins, And Total Phenolics, Eric L. Camera

Wine and Viticulture

Grapevines (cv Zinfandel) planted in the same vineyard 100 years apart were analyzed for juice chemistry and phenolic content of the fruit. The vineyard was dry farmed, and vines from the original 1890 vineyard were compared to vines inter-planted in 1990. As “old vine” wines can command higher prices in the market due to a perceived difference in fruit characteristics. This study attempted to observe if there was a measurable difference in fruit characteristics, or if it is simply an effective marketing strategy. There has been little research conducted in the wine industry attempting to observe a difference of old …

Influences Of Anthropogenic Noise On Flight Initiation Distance, Foraging Behavior, And Feeder Community Structure Of Wild Birds, Alissa R. Petrelli

Master's Theses

Throughout the world, birds represent the primary type of wildlife that people experience on a daily basis. However, a growing body of evidence suggests that alterations to the acoustic environment can negatively affect birds as well as humans in a variety of ways, and altered acoustics from noise pollution has the potential to influence human interactions with wild birds. In this thesis, I investigated how anthropogenic noise impacts daily behavior as well as community structure of wild birds. In the first component of this thesis, I assessed the distance at which a bird initiates flight or escape behavior (i.e., flight …

Agricultural Communications Lesson Plans For High School Agriculture Programs, Caitlin Anne Stanton

Agricultural Education and Communication

In today’s workforce, individuals are expected to be able to communicate effectively through all means of communication. This includes video, graphics, emails, social media, and in person. In the agriculture industry, these means of communication have become crucial to promoting agricultural literacy throughout the world. It is important for young people to have a grasp on professionally utilizing these communication methods so that they can use them in future careers as well as advocating for the agriculture industry. The role of technology and social media is rapidly increasing in everyday life and becoming a key learning tool in secondary education. …

Does Breastfeeding Shape Food Preferences? Links To Obesity, Alison K. Ventura

Kinesiology and Public Health

The first 2 years of life have been recognized as a critical window for obesity prevention efforts. This period is characterized by rapid growth and development and, in a relatively short period of time, a child transitions from a purely milk-based diet to a more varied solid-food diet. Much learning about food and eating occurs during this critical window, and it is well-documented that early feeding and dietary exposures predict later food preferences, eating behaviors, and dietary patterns. The focus of this review will be on the earliest feeding experiences - breast- and formula-feeding - and the unique role of …

Associations Between Bottle-Feeding Intensity And Maternal Encouragement Of Bottle-Emptying, Alison K. Ventura, Patsy Garcia, Andrew A. Schaffner

Kinesiology and Public Health

Objective: To explore longitudinal associations between bottle-feeding and maternal encouragement of infant bottle-emptying during the first 6 months of infancy.

Design: Mothers completed questionnaires during the third trimester of pregnancy, then monthly during the first 6 months postpartum. Questionnaires assessed family demographics, maternal and infant weight status, infant feeding patterns and maternal encouragement of infant bottle-emptying.

Setting: The Infant Feeding Practices Study 2, conducted by the US Centers for Disease Control and Prevention and the Food and Drug Administration.

Subjects: Mothers (n 1776).

Results: Repeated-measures regression was used to explore associations between bottle-feeding intensity (BFI; defined as the percentage …

Spatial And Temporal Heterogeneity Of Microbial Life In Artificial Landscapes, Roopkamal Kaur, Aditi Sengupta, Peter A. Troch

STAR Program Research Presentations

The Landscape Evolution Observatory (LEO) project at Biosphere 2 consists of three replicated artificial landscapes which are sealed within a climate-controlled glass house. LEO is composed of basaltic soil material with low organic matter, nutrients, and microbes. The landscapes are built to resemble zero-order basins and enables researchers to observe hydrological, biological, and geochemical evolution of landscapes in a controlled environment. This study is focused on capturing microbial community dynamics in LEO soil, pre- and post-controlled rainfall episodes. Soil samples were collected from six different positions and at five depths in each of the three slopes followed by DNA extraction …

Understanding The Transition From Benthic Egg To Dispersive Larvae: Observations On The Intra-Capsular Growth And Development Of A Marine Snail (Kelletia Kelletii), Megan N. Wilson

Biological Sciences

It has long been understood that the larval life stage is responsible for the dispersion of many marine organisms across their biogeographic range. Such organisms have a bipartite life cycle, existing in the water column and subject to oceanographic processes as planktonic larvae before settling to suitable habitat along the benthos where they grow and mature. Previous studies have demonstrated that larval growth rate and behavior in the water column can alter larval position in relation to ocean currents and affects their dispersal pathway. However, there is a paucity of information regarding the growth rate of the earliest larval stage …

Forest Densification Over 85 Years In A Sierra Nevada Mixed-Conifer Forest Decreases Conifer Regeneration And Limits Survival, Marissa A. Vossmer

Master's Theses

Forest densification in response to a century of fire suppression in Sierra Nevada mixed conifer forests has decreased conifer regeneration and survival. Increases in overstory biomass and decreases in canopy heterogeneity, along with decreases in shrub cover in the understory, has created unfavorable establishment site conditions for conifer species. Establishment site conditions are key in promoting germination and establishment of conifers seeds and in determining the survival of these seedlings into the overstory. These changes in establishment site suitability resulting from the removal of disturbance from these forests has decreased conifer regeneration and survival into other age classes. In Sierra …

Proteomic Analysis Of The Crustacean Molting Gland (Y-Organ) Over The Course Of The Molt Cycle, Talia B. Head

Master's Theses

Molting in crustaceans is a highly complex physiological process involving negative regulation by two paired endocrine glands, the X-organ/sinus gland complex (XO/SG) and the Y-organ (YO). The XO/SG complex is responsible for making molt-inhibiting hormone (MIH) which negatively regulates synthesis of the molting hormones, ecdysteroids, by the YO. Analysis of gene expression in the XOs and YOs has led to the development of a proposed molecular signaling pathway which regulates ecdysteroidogenesis and subsequent molting in crustaceans. In this study, changes in protein abundance in the YO were characterized over the course of a molt cycle (intermolt, early premolt, mid premolt, …

Assessing The Effect Of Nutritional Interventions On Body Composition Changes During Pregnancy In Moderately Malnourished Malawian Women, Megan K. Kitagawa

Master's Theses

Background: Malnutrition can affect the body composition changes that occur in healthy pregnancy, resulting in adverse effects to both the mother and infant. Supplementary foods with high quality protein may result in more favorable changes in lean mass and fat mass in pregnant malnourished women.

Objective: To retrospectively assess changes in body composition in moderately malnourished pregnant Malawian women in response to one of three nutritional interventions.

Methods: Height, weight, MUAC, and TSF were measured in pregnant women with MUAC >20.6 cm and19) were compared using ANOVA. If differences were detected, differences between groups were tested using the Tukey HSD …

Characterization Of Reagent Pencils For Deposition Of Reagents Onto Paper-Based Microfluidic Devices, Cheyenne H. Liu, Isabelle C. Noxon, Leah E. Cuellar, Amanda L. Thraen, Chad Immoos, Andres W. Martinez, Philip J. Costanzo

Chemistry and Biochemistry

Reagent pencils allow for solvent-free deposition of reagents onto paper-based microfluidic devices. The pencils are portable, easy to use, extend the shelf-life of reagents, and offer a platform for customizing diagnostic devices at the point of care. In this work, reagent pencils were characterized by measuring the wear resistance of pencil cores made from polyethylene glycols (PEGs) with different molecular weights and incorporating various concentrations of three different reagents using a standard pin abrasion test, as well as by measuring the efficiency of reagent delivery from the pencils to the test zones of paper-based microfluidic devices using absorption spectroscopy and …

Designing An Authentic Behavioral Research Experience In The Classroom Using Siamese Fighting Fish, Betta Splendens, Gianne Souza, Ronald M. Coleman

STAR Program Research Presentations

Recently there have been major shifts in biology education towards emphasizing the teaching of the scientific process to more closely reflect actual scientific practices. Authentic research experiences require the development of many higher order cognitive skills. Creating an authentic research experience that appropriately scaffolds the development of these complex skills while engaging individual student motivation remains challenging in classroom settings. Some common challenges include limited time available for working with large numbers of students, cramped working areas, and limited funding and materials resources. Using the Siamese Fighting Fish, Betta splendens, we have created an economically viable authentic animal behavioral …

Ecological And Organic Life Safety Concerns Of Hfo-1234yf, Alberni V. Ruiz

STAR Program Research Presentations

While at NOAA my research was directed by the inquiry of whether or not HFO-1234yf, a new compound being introduced into coolant system and replacing greenhouse gases HFC, is harmful to organic life. Using the computer program Matlab I was able to develop figures that represented the concentration of HFO-1234yf (HFO) in 12 different tower locations throughout the country. Comparing and contrasting concentrations of this HFO compound at each site led me to the question the possible harmful side effects this compound could bring to organic life due to the fact that concentration levels are increasing, with one factor being …

Strategies For Increasing The Release Of Pigments In Red Wine, Briana Heywood

Master's Theses

The perception of wine’s quality is directly influenced by its color. Anthocyanin molecules are responsible for imparting color to red wines. They are extracted from grape skins during alcoholic fermentation. This work compares the effects of three parameters: berry integrity, enzyme addition, and fermentation temperature, on phenolic compound extraction (total phenol, tannin and anthocyanin) during the production of Paso Robles’ Cabernet Sauvignon wine. Analyses on phenolic compounds were completed during alcoholic fermentation and barrel aging over the course of eighteen months. Berry integrity compared the degree of berry crushing (whole destemmed berries versus fully crushed berries). Results showed that phenolic …

Maternal Distraction During Breast- And Bottle Feeding Among Wic And Non-Wic Mothers, Alison K. Ventura, Simone Teitelbaum

Kinesiology and Public Health

Objective: To explore the prevalence and correlates of maternal distraction during infant feeding within a sample of mothers enrolled or not in the Special Supplemental Nutrition Program for Woman, Infants, and Children (WIC).

Design: Mothers kept diaries of their infants’ feeding patterns.

Participants: Mothers (n = 75) with infants aged ≤6 months.

Main Outcome Measures: Within the diaries, mothers recorded what else, if anything, they did during the feeding. Mothers also completed questionnaires on demographics, feeding styles, and infant temperament and eating behaviors.

Analysis: Mothers’ responses were coded into thematic categories. Feedings were classified as distracted when the mothers reported …

Heavy Metals In Seaweeds From A Polluted Estuary In Coastal Maine, Ian D. Medeiros, Arthur C. Mathieson, Nishanta Rajakaruna

Biological Sciences

Seaweeds (macroalgae) accumulate heavy metals from seawater and can therefore serve as biological monitors of marine pollution. In this paper, we present data on the tissue chemistry of seaweeds from the area of Callahan Mine, an intertidal copper and zinc mine in Brooksville, ME, USA, abandoned in the 1970s and now designated as a Superfund site. The mine is located on the Goose Pond estuary, which drains into the ocean via a tidal waterfall at Goose Falls. Seaweed tissue samples from this estuary had the same relative concentrations of metals as had been reported previously in water samples from this …

A Comparative Analysis Of The Nutrient Composition And Digestibility Of California Perennial And Annual Grasses At Four Stages Of Growth, Elaina Cromer

Master's Theses

Beef products represent the fourth largest agricultural commodity in the state of California, valuing more than $3 billion from 2013 to 2015 (CDFA, 2016) and procure 90% of the income for the range livestock industry (FRAP, 2003). Forages found on California’s coastal, desert, foothill, and mountain ranges are the basis of the state’s beef cattle industry. Understanding their nutritional quality of these forages is important for their effective use (George et al., 2001a; Waterman et al., 2014). The objectives of this research were to investigate the nutritional characteristics, and in situ digestbilities in Angus beef cattle, of common California annual …

Marine Biomass Analyzer, Kai Jay Staal, Brian Paris, Tyler Cottle

Mechanical Engineering

This report documents the design, fabrication, and testing for a marine biomass analyzer. The goal of the project and constructed system was to determine the validity and efficacy of a process that could flatten benthic macrofauna to a consistent thickness such that a biomass for the collected sample could be accurately determined. The system that was built and tested consists of a drive train that turns a mill subassembly where the organisms are flattened, supply and collection spools that hold the white fabric and clear film used to capture the organisms, a collection zone where the sample is injected, and …

Peromyscus Boylii And Peromysus Californicus Differ In Physical Characteristic Measurements, Puja M. Kasondra

Biological Sciences

I provide a comparison of physical characteristics between Peromyscus boylii and Peromyscus californicus. All mice compared were captured in the dominant regions of the Los Padres National Forest during three different years – 2005, 2011, and 2014. The dominant regions of the Los Padres National Forest include Riparian, Woodlands, and Chaparral. All characteristics, ear (from notch), head and body, hind foot, tail, and weights, were compared between males of each species and then between females of each species. All data were analyzed in JMP 12. Out of all the characteristics, tail and weight had the greatest difference between the …

Linking Large Scale Ocean-Atmospheric Patterns With Recruitment In Kellet’S Whelk (Kelletia Kelletii), Katherine N. Rodriguez

Biological Sciences

Global warming influences the biogeography of many marine and terrestrial species. Understanding species range shifts is ecologically and socioeconomically important when guiding management decisions for ecosystems exposed to a rapidly changing climate. In this natural experiment, I follow Danielle Zacherl’s methods (Zacherl et al. 2003) to study the effects of large-scale ocean-atmospheric patterns on recruitment of a marine snail, Kellet’s whelk (Kelletia kelletii); these organisms recently expanded their range North past the geographic barrier of Point Conception to Monterey, CA, USA. I use shell length data collected at 32 subtidal rocky reef kelp forest sites in 2015 (before …

Incorporating Instruction Into The Huasna Valley 4-H Beef Group, Carly P. Chandless

Agricultural Education and Communication

The purpose of this project was to design a manual to be used by 4-H leaders and members. The author wants to develop an interesting and intriguing manual that will demonstrate how to raise a market steer. This manual will contain step by step instructions on how fit a steer for show and how to halter break. These illustrations will help guide a 4-H member through the process of handling their steer. The Huasna Valley 4-H Steer Manual will provide beneficial information, to the leader and member, from the moment the project is purchased to sale. More importantly, the manual …

Hanford Ffa Ornamental Horticulture Unit Master Plan, Savanah Diane Rhine, Russell Alan Zimmerman

Agricultural Education and Communication

This project is intended to develop a more functional plan for the Ornamental Horticulture (OH) Unit at the Hanford Agriculture Program. The ag program combines all three high schools in Hanford resulting in over 1,000 students; however, a majority of these students are not in the OH Career Pathway due to a lack of a constant, fully functional unit. As a comprehensive agriculture program, it is crucial that it incorporates all aspects of agriculture, not just animal science and agricultural mechanics.

This project will provide the Hanford agriculture program with a plan to be implemented so that they may have …

Working Bodies: A Photo Essay On Tulare County Citrus Workers, Isamar Hernandez-Ramos

Agricultural Education and Communication

Many a times, when people think about their food, they think about origins. Where did it come from? Was it hygienic? Were the animals treated humanely? They seldom include the workers in their thought process, though the workers of the Ag industry provide what is arguably a vital part of the Ag industry. They do back-breaking labor in often lethal conditions for little pay (NFWM). The families of these workers often suffer economically, physically, and emotionally. In Tulare County, one of the top Agricultural counties in the country, the agricultural worker is often below the poverty line (Taylor, 2015). They …

Cost Of Feed Per Pound Of Gain In Cal Poly's Dairy Calves, Kayle Anna Phelan

Agricultural Education and Communication

California is ranked one of the top five states in the United States for dairy production. It produces approximately 40 billion pounds of milk per year for US production. California’s dairy industry is not only the largest milk producer in the nation, but it is also the most financially prominent in the agricultural industry. This industry lead is purely because of its careful management practices. The way calves are raised depend on the resources used, the natural environment present, and the operation of the dairy farm. Essentially, there are numerous ways to care for a cow and calf. However, the …

Assessing Soft Skills At The Cal Poly State Agriculture Ambassador Conference, Mary Luann Allen

Agricultural Education and Communication

In today’s society, there is a disconnect with the agriculture industry, agriculture schools, and the public. Students need to acquire soft skills to aid in furthering the agriculture industry through their careers. Overall, hiring managers found soft skills such as communication, leadership, ownership, and teamwork were missing in this new crop of workers (Payscale/Future Workplace, 2016).

College Agriculture Ambassadors advocate for agriculture, higher education, and their respective schools all across the United States. The Cal Poly Agriculture Ambassadors club provides leadership, communication, and personal development to its members through meetings, activities and responsibilities.

The Cal Poly Agriculture Ambassador club hosted …

Rabbit Showmanship Guidebook, Mitchell B. Liwanag

Agricultural Education and Communication

Rabbit Showmanship Guidebook

A Senior Project

Presented to

The Faculty of the Agricultural Education and Communication Department

California Polytechnic State University, San Luis Obispo

In Partial Fulfillment

of the Requirements for the Degree

Bachelor of Science

By

© Mitchell Liwanag

June 2017

Introduction: Every year 4-H, FFA, and Independent exhibitors compete in rabbit showmanship at hundreds of county and state fairs as well as rabbit shows. It is the author’s opinion with the proper guidance, showmanship can be simple and easy to learn. However, without the help of someone knowledgeable, showmanship is complicated. By creating a showmanship guide new leaders …

Developing Visual Aids For Ffa Promotion In The Classroom, Audrey Y. Lent

Agricultural Education and Communication

The purpose of this project is to create visual materials for a classroom that the teacher can use as a supplement for information taught about FFA activities and programs shared with students.

The FFA advisor whose chapter would be the beneficiary of the project was consulted to discuss the best subject material for the posters. One poster was created for Supervised Agricultural Experience (SAE) projects, one for Career and Leadership Development Events (CDEs and LDEs), one for the California FFA State Conference, and one for career opportunities in the agricultural industry. These four posters were developed using an online program …

Consumer Agricultural Education: Starting At The High School Level Health Class, Cecilia Teresa Acosta

Agricultural Education and Communication

The purpose of this project is to analyze and understand the communication gap between the farmer and consumer in order to create a dialogue about misconceptions in the media. Often rumors in the media worry or mislead the consumer in his or her buying choices. In order for consumers to understand what they are buying, and critically analyze media information, it is necessary to teach agricultural education at the high school level health class. This project will showcase how to start this education in order to bridge the communication gap starting with five crucial and common food-packaging labels. These labels …

The Recovery Of Protein From Egg Yolk Protein Extraction Granule Byproduct, Irene Jennifer Kaufman

Master's Theses

In addition to proving an excellent source of nutrients, eggs are used in the food, cosmetic, and biotechnology industries for their rheological and bioactive properties. Much of the potential for the added value is in individual components of the egg, rather than the whole egg. At low speed centrifugation, yolk separates into two distinct fractions—granules and plasma. It is becoming increasingly popular in the industry to remove the plasma fraction of the egg yolk to use for its livetins, particularly immunoglobulin Y, leaving behind a granule by-product (“yellow cake”). Previous research has shown potential added-value from the granule fraction, especially …

Pink And Dude Chefs: Efficacy Of An Online Train-The-Trainer Mechanism And Student Program Outcomes, Jacqueline Chen

Master's Theses

Background: The rate of overweight and obesity among adolescents aged 12-19 years has quadrupled since 1980. Reducing obesity is a key public health priority, as obesity is associated with individual and population-level health and economic consequences. Afterschool-based obesity prevention programs that aim to develop nutrition and culinary self-efficacy have shown promise. However, the level of aptitude among program instructors is directly correlated with student success, suggesting the importance of effective train-the-trainer (TTT) mechanisms for implementing and scaling up these strategies.

Pink and Dude Chefs (PDC) is an afterschool nutrition education and culinary skills program for middle-school adolescents aged between 11-14 …