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Articles 211 - 240 of 2796
Full-Text Articles in Civil and Environmental Engineering
Quantitative Sustainable Design (Qsd) For The Prioritization Of Research, Development, And Deployment Of Technologies: A Tutorial And Review, Yalin Li, John T. Trimmer, Steven Hand, Xinyi Zhang, Katherine G. Chambers, Hannah A. C. Lohman, Rui Shi, Diana M. Byrne, Sherri M. Cook, Jeremy S. Guest
Quantitative Sustainable Design (Qsd) For The Prioritization Of Research, Development, And Deployment Of Technologies: A Tutorial And Review, Yalin Li, John T. Trimmer, Steven Hand, Xinyi Zhang, Katherine G. Chambers, Hannah A. C. Lohman, Rui Shi, Diana M. Byrne, Sherri M. Cook, Jeremy S. Guest
UK CARES Faculty Publications
The pursuit of sustainability has catalyzed broad investment in the research, development, and deployment (RD&D) of innovative water, sanitation, and resource recovery technologies, yet the lack of transparent and agile methodologies to navigate the expansive landscape of technology development pathways remains a critical challenge. This challenge is further complicated by the higher levels of uncertainty that are intrinsic to early-stage technologies. In this work, we review and synthesize published literature on the sustainability analyses of water and related technologies to present quantitative sustainable design (QSD) – a methodology to expedite and support technology RD&D. With a shared lexicon and a …
Accuracy And Uncertainty In Traffic And Transit Ridership Forecasts, Jawad Mahmud Hoque
Accuracy And Uncertainty In Traffic And Transit Ridership Forecasts, Jawad Mahmud Hoque
Theses and Dissertations--Civil Engineering
Investments of public dollars on highway and transit infrastructure are influenced by the anticipated demands for highways and public transportations or traffic and transit ridership forecasts. The purpose of this study is to understand the accuracy of road traffic forecasts and transit ridership forecasts, to identify the factors that affect their accuracy, and to develop a method to estimate the uncertainty inherent in those forecasts. In addition, this research investigates the pre-pandemic decline in transit ridership across the US metro areas since 2012 and its influence on the accuracy of transit forecasts.
The sample of 1,291 road projects from the …
Evaluation Of Scoring Methods For Prioritizing Pedestrian And Bicycle Projects, Daria Korostina
Evaluation Of Scoring Methods For Prioritizing Pedestrian And Bicycle Projects, Daria Korostina
Theses and Dissertations--Civil Engineering
To increase the number of pedestrian and bicycle facilities and to grow the number of people using those facilities in Kentucky more such projects need to be implemented. The Strategic Highway Investment Formula for Tomorrow (SHIFT) is a data-driven approach that Kentucky uses for prioritizing projects in the state, but its focus is auto-centric. The purpose of this study was to develop and evaluate a prioritization scoring approach for pedestrian and bicycle projects that could be implemented into SHIFT. The study used the SHIFT–2022 pedestrian and bicycle projects to develop and evaluate different scoring scenarios. After scoring each project on …
Identifying And Evaluating The Perceptions Of Near-Miss Reporting Within The Kentucky Transportation Cabinet, Seth Atkins
Identifying And Evaluating The Perceptions Of Near-Miss Reporting Within The Kentucky Transportation Cabinet, Seth Atkins
Theses and Dissertations--Civil Engineering
In the field of construction, most safety data and practices focus on preventing and mitigating serious incidents resulting in injuries or fatalities. However, on construction sites, near-miss events occur more frequently than said serious incidents and, under marginally different conditions, could potentially lead to damages, injuries, or fatalities. Therefore, near-miss reporting can serve as a useful tool for managing safety as it allows for workers to identify and managers to address potential risk factors within construction sites. While most construction companies have implemented some method for reporting near-miss events, many organizations, such as the Kentucky Transportation Cabinet (KYTC), struggle with …
Evidence Of Coal Conversion In The Majuba Underground Coal Gasification Pilot Plant, South Africa, L.S. Mokhahlane, N.J. Wagner, D. Vermeulen
Evidence Of Coal Conversion In The Majuba Underground Coal Gasification Pilot Plant, South Africa, L.S. Mokhahlane, N.J. Wagner, D. Vermeulen
Coal Combustion and Gasification Products Journal Archive
Underground coal gasification (UCG) is a coal conversion method that permits coal resources to be exploited in situ using high-temperature conversion reactions. An understanding of the chemical, mineralogical, and petrographic properties of the coal, and resultant unburned carbon, degasified coal, and ash (minerals) in the UCG gasification zone, is fundamental to determining conversion rates, gas composition, and environmental and groundwater risk assessments. This study aims to provide a mineralogical and petrographic characterization of UCG residues obtained from the Eskom Majuba UCG pilot plant site in South Africa. Samples were selected from a verification borehole (VH3) drilled at the site following …
Low-Density And High-Strength Fracking Proppant Made By High Alumina Fly Ash, Shuqiang Ding, Jinder Jow
Low-Density And High-Strength Fracking Proppant Made By High Alumina Fly Ash, Shuqiang Ding, Jinder Jow
Coal Combustion and Gasification Products Journal Archive
High-alumina fly ash is defined to have alumina (Al2O3) content of no less than 40%, according to the Chinese power industry standard DL/T 2297. Specification for resource utilization categorization of fly ash from coal-fired power plant (Standard No. DL/T 2297-2021), and is considered a valuable raw material to replace bauxite for making aluminum, refractory materials, and fracking proppants, etc. A low-density and high-strength proppant, conventionally made with bauxite, has been successfully made by high-alumina fly ash and commercially used in the market. This new high-alumina f ly-ash–derived proppant has some advantages compared with the bauxite-derived proppant: (1) lower raw material …
Optimizing Commercial Vehicle Enforcement Investments And Activities To Improve Safety And Increase Revenue Collections, Brian Howell, Jennifer Walton, Andrew Martin
Optimizing Commercial Vehicle Enforcement Investments And Activities To Improve Safety And Increase Revenue Collections, Brian Howell, Jennifer Walton, Andrew Martin
Kentucky Transportation Center Research Report
The Kentucky Transportation Cabinet (KYTC) owns and maintains 14 fixed weigh stations for commercial vehicle enforcement. The Kentucky State Police (KSP) is responsible for staffing these facilities to conduct enforcement, while KYTC is responsible for constructing and maintaining these facilities. Like many states, Kentucky has experienced a decline of enforcement personnel to operate weigh stations limiting its ability to conduct inspections for safety enforcement and revenue collection. This study analyzes three CMV facilities for their impact on safety enforcement and revenue collection and determines their viability for potential replacement. All three existing weigh stations in Hardin, Fulton, and Henderson counties …
Development Of A Decision-Making Tool For Prediction Of Rainfall-Induced Landslides, Faisal Shakib Ahmed
Development Of A Decision-Making Tool For Prediction Of Rainfall-Induced Landslides, Faisal Shakib Ahmed
Theses and Dissertations--Civil Engineering
Landslides are frequently observed in mountainous places following prolonged periods of rain, frequently resulting in substantial topography changes. They pose a significant risk to human lives and the built environment globally, particularly in areas prone to excessive rainfall. While slope failures can occur because of human-caused factors such as slope loading or toe cutting for construction purposes, many failures occur because of rainfall penetrating an otherwise stable slope. A greater understanding of the characteristics and mechanics of landslides is consequently critical for geotechnical research, particularly in evaluating prospective mitigation strategies. The potential of slope failure is a primary consideration when …
Adapting Through Organizational Change In Capital Projects Organizations, Mahmoud El Jazzar
Adapting Through Organizational Change In Capital Projects Organizations, Mahmoud El Jazzar
Theses and Dissertations--Civil Engineering
Organizational change can be defined as an alteration of a core aspect of an organization’s operation, structure, or culture. Previous research on organizational change mainly has focused on different types of technical changes, such as alternative project delivery methods and strategies to adopt the design-build approach successfully. Also, previous studies have focused on the project level changes instead of organizational level responses. Here I show using a proposed change model and performing thematic, and analysis, that there exist common responses, and challenges across different types of capital projects organizations studied. The results show that responses to change will differ as …
Incorporating Speed Into Crash Modeling For Rural Two-Lane Highways, Fahmida Rahman
Incorporating Speed Into Crash Modeling For Rural Two-Lane Highways, Fahmida Rahman
Theses and Dissertations--Civil Engineering
Rural two-lane highways account for 76% in mileages of the total paved roads in the US. In Kentucky, these roads represent 85 % of the state-maintained mileages. Crashes on these roads account for 40% of all crashes, 47% of injury crashes, and 66% of fatal crashes on state-maintained roads. These statistics draw attention to the need to investigate the crashes on these roads. Several factors such as road geometries, traffic volume, human behavior, etc. contribute to crashes on a road. Recently, studies have identified speed as one of the key factors of crashes as well as the severity associated with …
Sewer Vapor Intrusion Pathway: Simulation Of Indoor Piping Systems And Operation Of Aroma-Voc Gas Analyzer, Hong Cheng Tay
Sewer Vapor Intrusion Pathway: Simulation Of Indoor Piping Systems And Operation Of Aroma-Voc Gas Analyzer, Hong Cheng Tay
Theses and Dissertations--Civil Engineering
Vapor Intrusion (VI) is a transport phenomenon that occurs when contaminants migrate from sub-surface sources into indoor spaces. Sewer tunnels, plumbing connections, and piping infrastructures can be important contributors to an increased potential of VI in a site, which results in indoor concentrations of volatile organic compounds (VOC). In the present work, the topic of sewer VI pathways were investigated through a prototype made from various types of indoor plumbing pipe fittings. This prototype simulates indoor piping systems and determine whether air and VOC were escaping through the pipe fittings. Moreover, this study is the first effort to use a …
Investigation Of Pfas Exposure Risks In Kentucky Using Mapping Tools, Sweta Ojha
Investigation Of Pfas Exposure Risks In Kentucky Using Mapping Tools, Sweta Ojha
Theses and Dissertations--Civil Engineering
Per- and polyfluoroalkyl substances (PFAS), a group of environmentally-persistent compounds, are environmentally ubiquitous and challenging to remediate. Several studies show that PFAS are detected in drinking water systems when sampling is conducted, making human exposure via drinking water an important health consideration. This research: 1) develops a mapping tool for prioritizing sampling locations; 2) establishes a method for making GIS data and meta(data) in the mapping tool accessible; 3) fosters decision making by integrating knowledge brokering and the alignment interest and influence matrix (AIIM). The tool developed is this research is a geospatial and statistical PFAS hot-spot screening model that …
Load And Pressure Distribution As A Function Of Dynamic Contact Stress At The Railroad Crosstie-Ballast Interface, Habib Abdil Unluoglu
Load And Pressure Distribution As A Function Of Dynamic Contact Stress At The Railroad Crosstie-Ballast Interface, Habib Abdil Unluoglu
Theses and Dissertations--Civil Engineering
Excessive crosstie wear and abrasion and ballast wear and fouling are two of the fundamental problems contributing to inadequate railroad track performance. This adversely affects the attainment and long-term maintenance of desired track geometric requirements. The magnitudes and distribution of the stresses at the crosstie-ballast (CT-B) interface must be known to determine the stress distribution on the ballast. However, the stresses at the top of the ballast often vary significantly. This study examines a new approach to predicting dynamic contact pressures at the interface of crosstie and ballast using the ‘square wave theory.’ A data set of in-track CT-B interfacial …
Understanding The Differences Between Industry Objectives And Institutional Learning, Kristen Schell
Understanding The Differences Between Industry Objectives And Institutional Learning, Kristen Schell
Theses and Dissertations--Civil Engineering
To combat understaffing in the construction industry, it is necessary to employ the best candidates possible. By identifying the most desirable skills in a construction employee from an industry perspective, institutional learning can better prepare graduates for the construction workforce. Currently there exists an information gap in the objectives of the construction industry and institutional learning. This gap produces graduates, professors, and employers with expectations that do not align. Reducing this gap will aid in the success of hiring recently graduated construction students who can meet the ever-changing demands of the industry. Construction students who are fresh out of college …
Optimizing The Potential Of Highway Safety Investment, Riana Tanzen
Optimizing The Potential Of Highway Safety Investment, Riana Tanzen
Theses and Dissertations--Civil Engineering
Highway safety management aims to prevent crashes and reduce the resulting frequency and severity within the limit of available resources. The identification of potentially hazardous sites and investment on safety treatments have been fundamental to fulfill this goal. However, having many highway safety improvement projects in hand, safety professionals need to evaluate several different alternatives and allocate the limited funds to the ones that would provide the highest return on investment. Hence, prioritizing the safety projects based on their potential to achieve the greatest safety benefit is crucial. Ineffective prioritization can distribute funds to locations with less potential for improvement …
Geophysics-Based Ground Control And Excavation Design Methodology Based On Acceptable Damage, Jorge Romana Giraldo
Geophysics-Based Ground Control And Excavation Design Methodology Based On Acceptable Damage, Jorge Romana Giraldo
Theses and Dissertations--Civil Engineering
This dissertation is structured around a novel conceptual framework for designing deep excavation retaining systems and using geophysical data to estimate the mechanical response of soft soil deposits. It begins with presenting an approach to design excavation retaining walls based on limiting damage to adjacent infrastructure. In this approach, the damage is defined based on critical distortions of an idealized laminate beam model used for representing the adjacent building deformations. The wall and support elements of the support system are then designed such that the system yields the limiting ground deformations. The resulting excavation support system limits damage to adjacent …
Induced Travel Demand: Measuring The Contribution Of Additional Lane Miles On The Increase In U.S. Vehicle Miles Traveled From 1980 To 2019, Brandon Ivanchak
Induced Travel Demand: Measuring The Contribution Of Additional Lane Miles On The Increase In U.S. Vehicle Miles Traveled From 1980 To 2019, Brandon Ivanchak
Theses and Dissertations--Civil Engineering
Induced travel demand is the effect of increasing the amount of vehicle miles traveled because of an increase in roadway capacity. It is explained by the idea that increasing capacity makes driving on those roads more desirable, thereby causing more people to use them. In1962, Robert Downs postulated that “On urban commuter expressways, peak hour traffic congestion rises to meet maximum capacity,” referring to this as the law of peak hour traffic congestion. Since then, there have been ongoing debates about the effectiveness and environmental impact of roadway expansion projects, and efforts to quantify induced demand to inform those debates. …
Sediment Nitrogen Dynamics In Backwater Wetland Confluences Of A Regulated River, Gina Degraves
Sediment Nitrogen Dynamics In Backwater Wetland Confluences Of A Regulated River, Gina Degraves
Theses and Dissertations--Biosystems and Agricultural Engineering
As harmful algal blooms in regulated river systems have increased in the past decade, the importance of understanding sediment nutrients has also increased. Research linking nutrient processes and fine sediment dynamics to harmful algal blooms in confluence wetlands along regulated rivers has recently become apparent. However, the relationship between sediment nutrient dynamics in confluence wetlands has been understudied. Utilization of sediment fingerprinting, high-frequency water quality monitoring, and tracer unmixing mass-balance modeling, was able to suggest sediment N mineralization in Appalachia confluence riparian wetland was not a dominate source of nitrate downstream. Further measures of supplementary tracers and additional sediment sources …
Classifying And Mapping Aquatic Vegetation In Heterogeneous Stream Ecosystems Using Visible And Multispectral Uav Imagery, Rozalia Agioutanti
Classifying And Mapping Aquatic Vegetation In Heterogeneous Stream Ecosystems Using Visible And Multispectral Uav Imagery, Rozalia Agioutanti
Theses and Dissertations--Biosystems and Agricultural Engineering
The need for assessment and management of aquatic vegetation in stream ecosystems is recognized given the importance in impacting water quality, hydrodynamics, and aquatic biota. However, existing approaches to monitor are laborious and its currently not feasible to track spatial and temporal differences at broad scales. The objective of this study was therefore to map and classify aquatic vegetation of a shallow stream with heterogenous mixtures of emergent and submerged aquatic vegetation. Data was collected in the Camden Creek watershed within the Inner Bluegrass Region of central Kentucky. The use of unmanned aerial vehicles (UAVs) was employed and both visible …
Assessing Machine Learning Utility In Predicting Hydrologic And Nitrate Dynamics In Karst Agroecosystems, Timothy Mcgill
Assessing Machine Learning Utility In Predicting Hydrologic And Nitrate Dynamics In Karst Agroecosystems, Timothy Mcgill
Theses and Dissertations--Biosystems and Agricultural Engineering
Seasonal hypoxia in the Gulf of Mexico and harmful algal blooms experienced in many inland freshwater bodies is partially driven due to excessive nitrogen loading seen from agricultural watersheds. Within the Mississippi/Atchafalaya River Basin, many areas are underlain with karst features, and efforts to reduce nitrogen contributions from these areas have had varying success, due to lacking a complete understanding of nutrient dynamics in karst agricultural systems. To improve the understanding of nitrogen cycling in these systems, 35 months of high resolution in situ water quality and atmospheric data were collected and fed into a two-hidden layer extreme learning machine …
Using Stream Restoration To Mitigate Stormwater Runoff In An Urban Watershed: A Case Study, Jonathan M. Brantley
Using Stream Restoration To Mitigate Stormwater Runoff In An Urban Watershed: A Case Study, Jonathan M. Brantley
Theses and Dissertations--Biosystems and Agricultural Engineering
The goal of this case study was to evaluate the effectiveness a 275 m regenerative stream restoration design to mitigate stormwater runoff in a highly urbanized watershed. The restoration resulted in a wide, wetland-like floodplain, comprised of a rock base that was overtopped with a filtration media (approximately 30% woodchips and 70% topsoil). The creation of the floodplain-wetland complex in tandem with filtration media lead to increased storage capacity and an increase in hyporheic exchange within the system. Significant reductions were found for the storm hydrograph parameters volume, peak discharge, and time to peak. Reductions in baseflows were also noted …
Protocols And Architecture For Privacy-Preserving Authentication And Secure Message Dissemination In Vehicular Ad Hoc Networks, Shafika Showkat Moni
Protocols And Architecture For Privacy-Preserving Authentication And Secure Message Dissemination In Vehicular Ad Hoc Networks, Shafika Showkat Moni
Theses and Dissertations--Computer Science
The rapid development in the automotive industry and wireless communication technologies have enhanced the popularity of Vehicular ad hoc networks (VANETs). Today, the automobile industry is developing sophisticated sensors that can provide a wide range of assistive features, including accident avoidance, automatic lane tracking, semi-autonomous driving, suggested lane changes, and more. VANETs can provide drivers a safer and more comfortable driving experience, as well as many other useful services by leveraging such technological advancements. Even though this networking technology enables smart and autonomous driving, it also introduces a plethora of attack vectors. However, the main issues to be sorted out …
Best Practices For Performance Measurement In Transportation Operations And Maintenance, Bryan Gibson, Rachel Catchings, Chris Van Dyke, Steve Waddle, Doug Kreis
Best Practices For Performance Measurement In Transportation Operations And Maintenance, Bryan Gibson, Rachel Catchings, Chris Van Dyke, Steve Waddle, Doug Kreis
Kentucky Transportation Center Research Report
Public agencies benefit from measuring their performance as it helps to focus employee and organizational activities. State departments of transportation have become more performance-oriented over the past two decades and routinely collect data on highway safety, infrastructure condition, system operations, project delivery, winter maintenance, transit, bicycle and pedestrian facilities, and customer service. While the Kentucky Transportation Cabinet (KYTC) use performance measures in a variety of areas, the agency wants to adopt new metrics related to mobility and the responsiveness of maintenance operations. This report documents performance measurement strategies used at state transportation agencies throughout the country and proposes new performance …
Integrating Construction 4.0 Technologies: A Four-Layer Implementation Plan, Mahmoud El Jazzar, Christian Schranz, Harald Urban, Hala Nassereddine
Integrating Construction 4.0 Technologies: A Four-Layer Implementation Plan, Mahmoud El Jazzar, Christian Schranz, Harald Urban, Hala Nassereddine
Civil Engineering Faculty Publications
This research explores the current state of Construction 4.0 and discusses a four-layer implementation of Construction 4.0 in the industry. The research methodology consists of an extensive literature review to gain insights about Construction 4.0 and frame the four-layer implementation plan. A case study is also presented to showcase the proposed implementation plan. Nine Construction 4.0 technologies were discussed, their integration throughout the project lifecycle was presented in a roadmap, their integration and connectivity with one another were outlined in an interaction roadmap, and the requirements necessary for achieving the 4.0 transformation were articulated. However, the proposed implementation plan is …
Effect Of Non-Structural Components On The Dynamic Response Of Steel-Framed Floors: Tests Before And After Component Installations, Mohammad Royvaran, Onur Avci, Brad Davis
Effect Of Non-Structural Components On The Dynamic Response Of Steel-Framed Floors: Tests Before And After Component Installations, Mohammad Royvaran, Onur Avci, Brad Davis
Civil Engineering Faculty Publications
The effect of partition walls and non-structural elements on the dynamic response of floors is still not well understood, and there is a need for vibration testing of floors at various stages of construction. The best way to shed some light on the effect of non-structural components is to test additional floors (preferably the same floor) before and after the installation of non-structural elements and compare the dynamic properties. For that purpose, the authors conducted vibration testing on a building floor under construction at various stages of fit-out to quantify the effects of various non-structural elements on the vibration response. …
Assessment Of Deteriorated Structural Concrete To Provide Durable Repairs, Theodore Hopwood, Christopher Goff, Sudhir Palle
Assessment Of Deteriorated Structural Concrete To Provide Durable Repairs, Theodore Hopwood, Christopher Goff, Sudhir Palle
Kentucky Transportation Center Research Report
Most of the structural elements on Kentucky bridges are made of reinforced concrete. Many of these elements deteriorate as a result of corrosion of the reinforcing steel caused by carbonation and — primarily — applications of chlorides by the Kentucky Transportation Cabinet (KYTC). Corrosion of reinforcing steel is reviewed along with assessment procedures that leverage nondestructive evaluation (NDE) methods and related laboratory and field tests. Several maintenance procedures are discussed, including the application of sealers and coatings, patch and major concrete repairs, and electrochemical methods. Incorporating NDE and test results into maintenance procedure selection is discussed. Recommendations are provided on …
Design Mythbusters, Christopher Van Dyke, Steve Waddle, Doug Kreis
Design Mythbusters, Christopher Van Dyke, Steve Waddle, Doug Kreis
Kentucky Transportation Center Research Report
When highway project designs depart from design values found in the Kentucky Transportation Cabinet (KYTC) Highway Design Manual and AASHTO’s A Policy on Geometric Design of Highways and Streets, project managers at the agency must obtain either a design exception or design variance. While designers are more comfortable with exceptions and variances than they were 10 or 20 years ago, some hesitancy remains, especially among the Cabinet’s consultants. Misperceptions about what exceptions or variances entail or about their performance may underlie this reluctance. Exceptions and variances are best conceptualized as formal justifications for design decisions. Project managers merely need …
A Review Of Kentucky’S Extended-Weight Hauling Programs, P. Gayle Marks, Jon Wilcoxson, Bryan Gibson, Chris Van Dyke, Andrew Martin, Jennifer Walton, Doug Kreis
A Review Of Kentucky’S Extended-Weight Hauling Programs, P. Gayle Marks, Jon Wilcoxson, Bryan Gibson, Chris Van Dyke, Andrew Martin, Jennifer Walton, Doug Kreis
Kentucky Transportation Center Research Report
Kentucky established its Extended Weight Coal or Coal By-products Haul Road System (EWCHRS) to increase the state’s competitiveness within the coal industry and reduce financial burdens on coal haulers. A new extended-weight haul system for unrefined petroleum products will come online in 2022. To facilitate enforcement of weight limits throughout Kentucky, this report surveys literature on how overweight vehicles affect roads and bridges, describes statutes and regulations governing weight limits in the state, discusses policies and strategies used throughout the US to handle overweight vehicles, and makes recommendations for improving extended-weight policies in Kentucky. It is apparent that pavements and …
Observed Risk And User Perception Of Road Infrastructure Safety Assessment For Cycling Mobility, Salvatore Cafiso, Giuseppina Pappalardo, Nikiforos Stamatiadis
Observed Risk And User Perception Of Road Infrastructure Safety Assessment For Cycling Mobility, Salvatore Cafiso, Giuseppina Pappalardo, Nikiforos Stamatiadis
Civil Engineering Faculty Publications
The opportunities for data collection in smart cities and communities provide new approaches for assessing risk of roadway components. This paper presents and compares two different methodological approaches for cycling safety assessment of objective and perceived risk. Objective risk was derived from speed and direction profiles collected with Global Navigation Satellite System (GNSS) and camera installed on an instrumented bicycle. Safety critical events between cyclists and other road users were identified and linked to five different roadway components. A panel of experts was asked to score the severity of the safety critical events using a Delphi process to reach consensus. …
Co-Carriage Of Metal And Antibiotic Resistance Genes In Sewage Associated Staphylococci, Atena Amirsoleimani, Gail M. Brion, Patrice Francois
Co-Carriage Of Metal And Antibiotic Resistance Genes In Sewage Associated Staphylococci, Atena Amirsoleimani, Gail M. Brion, Patrice Francois
Civil Engineering Faculty Publications
Controlling spread of resistance genes from wastewater to aquatic systems requires more knowledge on how resistance genes are acquired and transmitted. Whole genomic sequences from sewage-associated staphylococcus isolates (20 S. aureus, 2 Staphylococcus warneri, and 2 Staphylococcus delphini) were analyzed for the presence of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs). Plasmid sequences were identified in each isolate to investigate co-carriage of ARGs and MRGs within. BLASTN analysis showed that 67% of the isolates carried more than one ARG. The carriage of multiple plasmids was observed more in CC5 than CC8 S. aureus strains. Plasmid …