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Articles 1 - 30 of 39
Full-Text Articles in Other Biomedical Engineering and Bioengineering
Impact Of Cysteine And Tyrosine Dipeptides On Cho Cell Performance In A Perfusion Mimic, Corrin L. Pruitt
Impact Of Cysteine And Tyrosine Dipeptides On Cho Cell Performance In A Perfusion Mimic, Corrin L. Pruitt
All Theses
Perfusion cell cultures typically achieve higher cell densities and generate higher volumetric productivities than fed-batch cultures due to continuous nutrient supply and spent media removal. Despite these advantages, perfusion cell cultures remain underutilized for licensed product manufacturing, primarily due to the lack of small-scale perfusion models for high-throughput process development and the complexities involved in media formulation capable of supporting high cell densities during long-term continuous operation. To avoid nutrient limitation at high cell densities, current perfusion processes rely on using higher perfusion rates, which results in significant media wastage and downstream product dilution. Therefore, there has been an effort …
Investigating The Role Of Fluid Shear Stress During Metastasis On Enhanced Breast Cancer Extravasation, Alejandra M. Ham
Investigating The Role Of Fluid Shear Stress During Metastasis On Enhanced Breast Cancer Extravasation, Alejandra M. Ham
LSU Master's Theses
In 2022, ~288,000 American women were diagnosed with metastatic breast cancer which was the leading cause of patient mortality with ~43,000 American women succumbing to the disease. Women can be diagnosed with four different molecular subtypes of breast cancer: luminal A, luminal B, human epithelial growth receptor type 2 enriched (HER2+), and triple-negative breast cancer (TNBC). TNBC accounts for ~12% of all breast cancers, yet it has the worst outcomes. TNBC is highly invasive, with ~46% of TNBC patients developing metastatic disease. A further understanding of metastatic TNBC is needed to provide a greater understanding of how to treat this …
Physiologically Relevant Fluid Shear Stress Induced Receptor Conversion In Hormone Receptor-Positive (Hr+) Breast Cancer, Jonathan James Cuccia
Physiologically Relevant Fluid Shear Stress Induced Receptor Conversion In Hormone Receptor-Positive (Hr+) Breast Cancer, Jonathan James Cuccia
LSU Doctoral Dissertations
Breast cancer is the most common type of cancer among all people in the United States as of 2023 and often has devastating impacts on afflicted patients. Although some treatments show great efficacy against primary breast tumors, none have been able to significantly reduce the mortality rate of breast cancer once it has metastasized to distant sites. In primary, hormone receptor-positive (HR+) breast cancers, endocrine therapies are often used as first-line treatments and are generally well-tolerated. However, as the disease progresses, these previously sensitive cancers can become resistant to endocrine therapies and continue proliferating despite them. Therefore, there is a …
Antimicrobial Activity And Mechanism Of Amyloid Proteins And Synthetic Conjugated Polyelectrolytes, Fahimeh Maghsoodi
Antimicrobial Activity And Mechanism Of Amyloid Proteins And Synthetic Conjugated Polyelectrolytes, Fahimeh Maghsoodi
Nanoscience and Microsystems ETDs
Although the exact cause of Alzheimer’s disease (AD) is still unknown, it is widely considered that the accumulation of amyloid plaques composed of the amyloid-β (Aβ) peptide in the brain is linked to neurodegeneration. Co-localization of viral DNA with Aβ plaques, the association of brain infection and AD, and research indicating the protective effect of Aβ against bacteria and fungi in mice and human cells have led to the hypothesis that Aβ expression and deposition may be central to its function as an antimicrobial peptide (AMP). In my thesis research, we seek to elucidate how Aβ functions as an AMP …
Investigating Myosin Ensemble Force Generation Using Optical Tweezers, Janie Watts
Investigating Myosin Ensemble Force Generation Using Optical Tweezers, Janie Watts
Honors Theses
Myosin is a motor protein that facilitates muscle contraction and movement by stepping along actin filaments using energy from ATP hydrolysis. If myosin or other motors are disrupted throughout the body, it can have many harmful effects. Hypertrophic cardiomyopathy is a disease caused by gene mutations that affect myosin heavy chains in the heart. The tissue of the heart becomes abnormally thick, which can make it more difficult to pump blood or block blood flow out of the heart. Our goal is to discern the mechanistic difference function of a healthy and diseased heart. To accomplish this, we construct model …
Development Of Light Actuated Chemical Delivery Platform On A 2-D Array Of Micropore Structure, Hojjat Rostami Azmand, Hojjat Rostami Azmand
Development Of Light Actuated Chemical Delivery Platform On A 2-D Array Of Micropore Structure, Hojjat Rostami Azmand, Hojjat Rostami Azmand
Dissertations and Theses
Localized chemical delivery plays an essential role in the fundamental information transfers within biological systems. Thus, the ability to mimic the natural chemical signal modulation would provide significant contributions to understand the functional signaling pathway of biological cells and develop new prosthetic devices for neurological disorders. In this paper, we demonstrate a light-controlled hydrogel platform that can be used for localized chemical delivery in a high spatial resolution. By utilizing the photothermal behavior of graphene-hydrogel composites confined within micron-sized fluidic channels, patterned light illumination creates the parallel and independent actuation of chemical release in a group of fluidic ports. The …
Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian
Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian
Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research
The development of recombinant-based liquid fibrin tissue sealants having enhanced hemostatic and wound healing properties will involve understanding as yet not well characterized interactions between fibrinogen, fibrin (Fbn) factor XIII, thrombin and fibronectin. We study these phenomena in the context of comparing plasma derived fibrinogen to recombinant fibrinogen (rFI) produced in the milk of transgenic cows. An abundance of purified γγ and γγ’ FI subspecies enables detailed study of γγ or γγ’ biomonomer and their respective Fbn biopolymer formation as having different substrate behaviors of activated plasma derived factor XIII (pFXIIIa2b2). High pressure size exclusion (HPSEC) …
Intensification Of Gas Absorption In A Downward Flow Microbubble Bioreactor, Manizheh Ansari
Intensification Of Gas Absorption In A Downward Flow Microbubble Bioreactor, Manizheh Ansari
Dissertations and Theses
Bioreactors are of interest for value-upgrading of stranded or waste industrial gases, such as CO, CH4, or syngas. Process economics requires reduction of reactor cost and size while maintaining high production rate via rapid delivery of gas feedstock to the liquid phase which in turn requires a high volumetric mass transfer rate (). One strategy to achieve this goal is to increase interfacial area density, a, to >3000 m2 m-3, while minimizing use of energy. Here we show a novel reactor column design that uses a micro-jet array to break feedstock at ambient pressure …
Effects Of Micro-Features On Cell Detachment From Poly(N-Isopropylacrylamide) Coated Polydimethylsiloxane Membranes, Luke Webel
Williams Honors College, Honors Research Projects
The tested hypothesis was that features on polydimethylsiloxane (PDMS) surfaces coated with a poly(N-isopropylacrylamide)/aminopropyltriethoxysilane or pNIPAAM/APTES thin film would accelerate cell detachment than the film coated on a unfeatured surface. This project tested samples with features generated by molds, wrinkling, and sandpaper roughened substrates. Surface feature generation methods were limited to mechanical means, and characterized by microscopy and strain rates. 50/50 mixtures of 1.5 wt.% pNIPAAM/ APTES were used to coat thin films (30-40 nm) on PDMS membranes by spin-coating, and the coated membranes were thermally annealed to chemically graft pNIPAAm/APTES on the membrane and their thermo-responsive property was assessed …
Direct Quantification Of Deubiquitinating Enzyme Activity In Single Intact Cells, Nora Safabakhsh
Direct Quantification Of Deubiquitinating Enzyme Activity In Single Intact Cells, Nora Safabakhsh
LSU Doctoral Dissertations
Challenges in drug efficacy occur during the treatment of most types of cancer due to the heterogeneity of the tumor microenvironment. This has led to the development of personalized medicine. Due to the clinical success of the proteasome inhibitors Bortezomib and Carfilzomib in treatment of multiple myeloma, interest has shifted towards molecularly-targeted chemotherapeutics for ubiquitin-proteasome system (UPS). Deubiquitinating enzymes (DUBs) are an essential part of this pathway which have been found to promote Bortezomib resistance in multiple myeloma patients. Unfortunately, there is a lack of specific, high throughput biochemical assays to characterize DUB activity in patient samples before and after …
Distribution And Localization Of Novel Iodine Nanoparticles In The Human Glioma 1242 Growing In The Brains Of Mice, Benjamin Billings
Distribution And Localization Of Novel Iodine Nanoparticles In The Human Glioma 1242 Growing In The Brains Of Mice, Benjamin Billings
Honors Scholar Theses
Observing and designing the in vivo distribution and localization of therapeutic nanoparticles is an essential aspect of developing and understanding novel nanoparticle- based medical treatments. This study investigates novel PEGylated Iodine-based nanoparticles (INPs), an alternate composition to the more widely researched gold nanoparticles (AuNPs), which may help avoid adverse effects associated with AuNPs, such as potential toxicity and skin discoloration, when used in similar applications. Determining the localization of the novel INPs within murine brains containing human glioma U-1242MG cells is critical in assisting the development of radiation dose enhancement therapy for this aggressive cancer. Radiation dose enhancement utilizes the …
Preparation Of Supramolecular Amphiphilic Cyclodextrin Bilayer Vesicles For Pharmaceutical Applications, Kate E. Frischkorn
Preparation Of Supramolecular Amphiphilic Cyclodextrin Bilayer Vesicles For Pharmaceutical Applications, Kate E. Frischkorn
Master's Theses
Recent pharmaceutical developments have investigated using supramolecular nanoparticles in order to increase the bioavailability and solubility of drugs delivered in various methods. Modification of the carbohydrate cyclodextrin increases the ability to encapsulate hydrophobic pharmaceutical molecules by forming a carrier with a hydrophobic core and hydrophilic exterior. Guest molecules are commonly added to these inclusion complexes in order to add stability and further increase targeting abilities of the carriers. One such guest molecule is adamantine combined with a poly(ethylene glycol) chain. Vesicles are formed by hydrating a thin film of amphiphilic cyclodextrin and guest molecules in buffer solution that mimics physiological …
Development Of An In Vitro Platform For The Exploration Of Skeletal Muscle Cell Mechanobiology, Sydney Spradlin
Development Of An In Vitro Platform For The Exploration Of Skeletal Muscle Cell Mechanobiology, Sydney Spradlin
Biomedical Engineering Undergraduate Honors Theses
Muscles have the natural ability to regenerate when damaged. This works to keep the body functioning, unless this ability is overwhelmed. Volumetric muscle loss (VML) occurs when a substantial amount of a muscles mass is lost. This leads to the development of scar tissue, and often a loss of function. In order to find a solution for this condition, in vitro models are needed to explore the mechanobiology of skeletal muscle cells. Two platforms were developed in this study, to explore 3 factors: orientation, strain, and supporting structure. One platform used fibronectin stamped lanes on PDMS to encourage cell growth …
Characterization Of Molecular Communication Based On Cell Metabolism Through Mutual Information And Flux Balance Analysis, Zahmeeth Sayed Sakkaff
Characterization Of Molecular Communication Based On Cell Metabolism Through Mutual Information And Flux Balance Analysis, Zahmeeth Sayed Sakkaff
School of Computing: Dissertations, Theses, and Student Research
Synthetic biology is providing novel tools to engineer cells and access the basis of their molecular information processing, including their communication channels based on chemical reactions and molecule exchange. Molecular communication is a discipline in communication engineering that studies these types of communications and ways to exploit them for novel purposes, such as the development of ubiquitous and heterogeneous communication networks to interconnect biological cells with nano and biotechnology-enabled devices, i.e., the Internet of Bio-Nano Things. One major problem in realizing these goals stands in the development of reliable techniques to control the engineered cells and their behavior from the …
Subcellular And In-Vivo Nano-Endoscopy, Surya Venkatasekhar Cheemalapati, John Winskas, Hao Wang, Karthik Konnaiyan, Arseny Zhdanov, Alison Roth, Swamy Rakesh Adapa, Andrew Deonarine, Rays H. Y. Jiang, Anna Pyayt
Subcellular And In-Vivo Nano-Endoscopy, Surya Venkatasekhar Cheemalapati, John Winskas, Hao Wang, Karthik Konnaiyan, Arseny Zhdanov, Alison Roth, Swamy Rakesh Adapa, Andrew Deonarine, Rays H. Y. Jiang, Anna Pyayt
Chemical, Biological and Materials Engineering Faculty Publications
Analysis of individual cells at the subcellular level is important for understanding diseases and accelerating drug discovery. Nanoscale endoscopes allow minimally invasive probing of individual cell interiors. Several such instruments have been presented previously, but they are either too complex to fabricate or require sophisticated external detectors because of low signal collection efficiency. Here we present a nanoendoscope that can locally excite fluorescence in labelled cell organelles and collect the emitted signal for spectral analysis. Finite Difference Time Domain (FDTD) simulations have shown that with an optimized nanoendoscope taper profile, the light emission and collection was localized within ~100 nm. …
Local Delivery Of Ctla-4 Blockade Inhibits Growth Of Pancreatic Tumors, Jack Baltz
Local Delivery Of Ctla-4 Blockade Inhibits Growth Of Pancreatic Tumors, Jack Baltz
Biomedical Engineering Undergraduate Honors Theses
Immune checkpoint blockade has demonstrated great potential in activating antitumor immunity. Ipilimumab is a monoclonal antibody which targets cytotoxic T-lymphocyte antigen-4. CTLA-4 belongs to the CD28 class of receptors and is found on the surface of CD4+ and CD8+ T cells. CTLA-4 acts to suppress the immune system when bound to CD80 and CD86 receptors on antigen presenting cells. Ipilimumab, or anti-CTLA-4, has shown to be effective in significantly extending the survival of patients with metastatic melanoma. However, systemic delivery of Ipilimumab also induces significant side effects such as: colitis, dermatitis, uveitis, and hypophysitis. In order to minimize …
Characterization Of Novel Chitosan/Polyelectrolyte Nanoparticles, Michaela Wynne Mertz
Characterization Of Novel Chitosan/Polyelectrolyte Nanoparticles, Michaela Wynne Mertz
Biomedical Engineering Undergraduate Honors Theses
Chitosan-based nanoparticles are under investigation as delivery vehicles for drugs, genes, and proteins. Due to the biocompatibility, mucoadhesive nature, and biodegradable nature of chitosan and binding qualities, anionic nature, and biocompatible qualities of heparin, chitosan/heparin nanoparticles (CHNPs) are a promising platform for the delivery of labile proteins. However, the literature lacks a definitive process to control the size, efficiency, and yield of CHNPs at higher concentrations of reagents. Additionally there is a need to improve loading efficiency of CHNPs. In this study CHNPs have been synthesized and characterized for size, yield, protein loading efficiency, and release. The CHNPs were prepared …
Evaluating The Viability Of Graphene Oxide For The Removal Of Ni(Ii) Ions From Waste Water, Cale M. White
Evaluating The Viability Of Graphene Oxide For The Removal Of Ni(Ii) Ions From Waste Water, Cale M. White
Biomedical Engineering Undergraduate Honors Theses
When levels of heavy metal ions such as nickel (Ni), copper (Cu), lead (Pb), Zinc (Zn), and Cobalt (Co) exceed the tolerance values for industrial wastewater, it can devastate the environment as well as the health of the individuals consuming the water. Heavy metal ions are not biodegradable and will accumulate in organic matter.(1) This accumulation leads to toxic levels in the blood and hence damage to nerve tissue, kidneys, the liver, and other vital organs. (2) Effective removal processes of these heavy metals from water must be developed. This study explores a method to adsorb nickel from water with …
Fractionation Of Chitosan Using A Novel Precipitation Method, Davis J. Ward
Fractionation Of Chitosan Using A Novel Precipitation Method, Davis J. Ward
Biomedical Engineering Undergraduate Honors Theses
Chitosan is a derivative of chitin, a biopolymer found in the exoskeletons of crustaceans. Chitosan is under heavy investigation in the field of drug delivery due to its wide versatility. One such delivery mechanism is the use of hydrogels in cancer immunotherapy delivery. The viscosity of chitosan in its use as a hydrogel depends on the length of chitosan’s N-acetyl D-glucosamine chains, which in turn determines the molecular weight of chitosan. Because there is such a wide range of viscosity in a typical sample of chitosan, there is a need for a simple technique to fractionate the chitosan based on …
The Investigation Of Chitosan-Based Hydrogels For Intratumoral Delivery Of Immunotherapeutic Cytokines, Ethan Drew Lowry
The Investigation Of Chitosan-Based Hydrogels For Intratumoral Delivery Of Immunotherapeutic Cytokines, Ethan Drew Lowry
Biomedical Engineering Undergraduate Honors Theses
Controlled and localized delivery of Interleukin 12 (IL-12) is critical to effective and non-toxic cancer immunotherapy. Current delivery protocols for pre-clinical anti-tumor trials utilize solutions of chitosan, a mucoadhesive and bioeliminable biopolymer, to slow the release of IL- 12 into the tumor microenvironment. In order to further increase IL-12 retention, we have developed and characterized two chitosan-based in situ gelling hydrogels for IL-12 immunotherapy. One hydrogel, composed of heparin and chitosan, is herein described from initial conception through multiple stages of characterization including isolation of a range of suitable hydrogel formulations and observational physical and chemical properties. A second hydrogel …
Fluorescent Imaging On A Microfluidics Chip For Quantification Of Leukocyte Count, Aneeka Majid
Fluorescent Imaging On A Microfluidics Chip For Quantification Of Leukocyte Count, Aneeka Majid
Biomedical Engineering Undergraduate Honors Theses
We have demonstrated a method for conducting a leukocyte count in whole blood using a microfluidics chip and epi-fluorescence setup. Leukocyte counts provide physicians with a wealth of information about a patient’s medical condition and as such are routinely completed for many hospital visits. Miniaturization of this diagnostic tool may enable physicians to provide healthcare in resource-limited settings, where patients would otherwise not receive this test. The microfluidics chip was fabricated in polydimethylsiloxane (PDMS) using soft-film lithography. Following further processing and cleaning, the PDMS mold is exposed to UV-ozone for surface activation, and then sealed with a glass coverslip to …
Monitoring The Effects Of Mmp Inhibitors On Extracellular Matrix Degradation For Use In Implant Protection, Jake D. Jones
Monitoring The Effects Of Mmp Inhibitors On Extracellular Matrix Degradation For Use In Implant Protection, Jake D. Jones
Biomedical Engineering Undergraduate Honors Theses
In order to lengthen the functional time of implantable ECM, the mechanism of degradation must be inhibited. The matrix metalloproteases are the primary method by which ECM is broken down, and the TIMP family of proteins exists as proof that inhibition of these proteases is possible naturally. In particular, the glycoprotein TIMP-1 plays an inhibitory role with most of the MMPs including the collagenases. Furthermore, there exist synthetic or chemical molecules that likewise inhibit the catalytic actions of MMPs. One such molecule is Batimastat (BB-94) which has been proven to be a dose dependent inhibitor of MMPs during the inflammatory …
Development Of An In Vitro Myogenesis Assay, Anna Arnaud
Development Of An In Vitro Myogenesis Assay, Anna Arnaud
Biomedical Engineering Undergraduate Honors Theses
The objective of this study was to explore the interaction between mouse C2C12 cells and the extracellular matrix, particularly the process of myoblasts converting to myocytes. This study aimed to create a myogenesis assay that presents a process to effectively monitor the development of mouse C2C12 myoblasts into differentiated skeletal myotubes through detection of the protein MyoD. Myogenesis, the development of muscle tissue, occurs when muscle progenitor cells, myoblasts, fuse to form multinucleated myotubes, followed by cell fusion and resulting in a myofiber capable of contraction. An in vitro myogenesis assay would enable further research to efficiently test the effect …
Multiplexed Microfluidic Loop-Mediated Isothermal Amplification Of The 16s Rrna Gene For The Diagnosis Of Neonatal Sepsis In Resource-Limited Environments, Griffin Thomas Sonaty
Multiplexed Microfluidic Loop-Mediated Isothermal Amplification Of The 16s Rrna Gene For The Diagnosis Of Neonatal Sepsis In Resource-Limited Environments, Griffin Thomas Sonaty
Biomedical Engineering Undergraduate Honors Theses
Bacterial infection, or sepsis, places a disproportionately high burden on newborns in developing countries. This is due in part to a lack of diagnostic tools suitable for sustainable use in resource-limited nurseries. One potential vehicle for a new diagnostic assay is loop-mediated isothermal amplification (LAMP), a high-yield DNA amplification method. LAMP has been used to detect single genes from bacteria in blood serum samples to aid in sepsis diagnosis. While specific, this approach can only provide detection for one species at a time. LAMP could be adapted to detect a broader set of bacteria, while retaining a degree of specificity …
Intracellular Uptake Of Chitosan Nanoparticle-Based Vaccines, Katherine E. Wilson
Intracellular Uptake Of Chitosan Nanoparticle-Based Vaccines, Katherine E. Wilson
Biomedical Engineering Undergraduate Honors Theses
Particle-based delivery systems are a promising approach to enhance antigen-specific immunity for use in vaccines. Chitosan, a natural polysaccharide derived from the exoskeletons of crustaceans, shows great potential as a vaccine carrier due to its favorable properties as a biomaterial, including biocompatibility and biodegradability. In this study, the intracellular uptake of chitosan nanoparticles is explored and compared to that of other commonly used particle-based delivery vehicles, such as poly (lactic-co-glycolic acid) (PLGA). Fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) was used as a model protein antigen and encapsulated in both chitosan particles via precipitation-coacervation and PLGA particles via double emulsion solvent …
Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes
Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes
Master's Theses
The American Cancer Society predicts that 1,665,540 people will be diagnosed with cancer, and 585,720 people will die from cancer in 2014. One of the most common types of cancer in the United States is skin cancer. Melanoma alone is predicted to account for 10,000 of the cancer related deaths in 2014. As a highly mobile and aggressive form of cancer, melanoma is difficult to fight once it has metastasized through the body. Early detection in such varieties of cancer is critical in improving survival rates in afflicted patients. Present methods of detection rely on visual examination of suspicious regions …
Validation Of A Diffuse Reflectance Microendoscope With Polydimethylsiloxane Optical Phantoms And Monte Carlo Modeling, Gage Joseph Greening
Validation Of A Diffuse Reflectance Microendoscope With Polydimethylsiloxane Optical Phantoms And Monte Carlo Modeling, Gage Joseph Greening
Biomedical Engineering Undergraduate Honors Theses
We have introduced a method for creating thin-film PDMS optical phantoms that can be used to validate a bench-top optical device called a Diffuse Reflectance Microendoscope (DRME). The DRME is acquires data on cell surface morphology and sub-surface diffuse reflectance, which may provide clinicians with the tools to diagnose oral cancer at earlier stages. Phantoms were created with PDMS. Optical properties of the phantoms were controlled with the addition of nigrosin and titanium dioxide. Depth of phantoms was controlled by a spin coater. The DRME was built with a combination of filters and dichroic mirrors to separate electromagnetic radiation in …
Poly (Glycerol Sebacate) Nanofibers And Nanofilms For Tissue Engineering Application, Stephanie Grace Cone
Poly (Glycerol Sebacate) Nanofibers And Nanofilms For Tissue Engineering Application, Stephanie Grace Cone
Biomedical Engineering Undergraduate Honors Theses
In the field of tissue engineering, the development of biodegradable scaffolds that provide both structure and functionality is a major challenge. The use of biological implants, such as decellularized tissues, provides a complete matrix with full tissue functionality, however, problems with immunogenicity often arise when implants from other organisms are used in treatments. As such, there are many benefits to the use of polymeric constructs in tissue engineering. The ability to customize the material, design, and size of an implant provides opportunities for increased structural support and controlled rates of biodegradation. The selection of polymers for tissue engineering applications requires …
Classification And Quantification Of Phenotypic Characteristics Of Normal And Dysplastic Oral Cells, Jackson W. Boice
Classification And Quantification Of Phenotypic Characteristics Of Normal And Dysplastic Oral Cells, Jackson W. Boice
Biomedical Engineering Undergraduate Honors Theses
Introduction Oral cancer accounts for 2.5% of all cancer cases in the United States. The 5 year survival rate or stage 1 or localized oral cancer is 82%, and the survival rate of unstaged oral cancer is only 50%; therefore, early diagnosis is key. Current clinical practices for determining malignancy of tissue include physical examination and surgical biopsy, which can be plagued by high variability between observers and are invasive for the patient. Proflavine intercalates between base pairs of nucleic acids and has excitation and emission peaks at 455 (+/- 20) nm and 515 nm respectively; therefore, it can be …
Optimizing In Vitro Extracellular Matrix Production Using Polymer Scaffolds With Targeted Pore Size, Addison M. Walker
Optimizing In Vitro Extracellular Matrix Production Using Polymer Scaffolds With Targeted Pore Size, Addison M. Walker
Biomedical Engineering Undergraduate Honors Theses
Extracellular matrix (ECM) has been shown to provide the framework needed for healthy tissue to grow after experiencing an injury. The optimization of growing ECM is a pressing concern in the tissue regeneration field. From prior research, an in vitro approach for harvesting ECM has been created using polyurethane (PU) foams seeded with cells. Our approach aimed at discovering if the pore size of the foams increased the amount of recovered ECM. Scaffolds of targeted pore size were created by separating sugar granules using a sieve, packing the sugar into a cylindrical mold, then pouring PU over the sugar. Three …