Biomedical Engineering and Bioengineering Commons^™

Radiopaque Femnn-Mo Composite Drawn Filled Tubing Wires For Braided Absorbable Neurovascular Devices, Adam J. Griebel, Petra Maier, Henry D. Summers, Benjamin Clausius, Isabella Kanasty, Weilue He, Nicholas Peterson, Carolyn Czerniak, Alexander A. Oliver, David F. Kallmes, Ramanathan Kadirvel, Jeremy E. Schaffer, Roger J. Guillory

Michigan Tech Publications

Flow diverter devices are small stents used to divert blood flow away from aneurysms in the brain, stagnating flow and inducing intra-aneurysmal thrombosis which in time will prevent aneurysm rupture. Current devices are formed from thin (∼25 μm) wires which will remain in place long after the aneurysm has been mitigated. As their continued presence could lead to secondary complications, an absorbable flow diverter which dissolves into the body after aneurysm occlusion is desirable. The absorbable metals investigated to date struggle to achieve the necessary combination of strength, elasticity, corrosion rate, fragmentation resistance, radiopacity, and biocompatibility. This work proposes and …

Multi-Functional Gelatin-Dithiolane Hydrogels For Tissue Engineering, Saad Asim, Cody Tuftee, Asma Talib Qureshi, Rachel Callaghan, Moira L. Geary, Mithun Santra, Vaibhav Pal, Ilayda Namli, Gary Hin Fai Yam, Ibrahim T. Ozbolat, Muhammad Rizwan

Michigan Tech Publications

Biomaterials that integrate multiple functionalities, mimic the extracellular matrix (ECM) microenvironment to support cellular growth, and adhere robustly to damaged tissues are highly needed to advance tissue engineering. Protein-based biomaterials are promising due to their inherent biocompatibility, biomimicry, biodegradation, and cell-supportive properties. Herein, by leveraging the unique ability of dithiolanes to generate on-demand in situ thiols, a new class of dithiolane-modified, protein-based biomaterial that combines unique, seemingly opposing functions for tissue engineering is developed. Dithiolane-modified gelatin, a model protein used herein, enabled photoinitiator-free photo-crosslinking to form multi-functional gelatin-dithiolane (GelDT) hydrogels, which displayed exceptional long-term stability in cell culture media ( …

Salicylhydroxamic Acid Containing Structural Adhesive, Md Saleh Akram Bhuiyan, Kan Wang, Seyedehfatemeh Razaviamri, Bruce Lee

Michigan Tech Publications

The feasibility of utilizing salicylhydroxamic acid (SHAM) as a new adhesive molecule for designing structural adhesives is investigated in this study. SHAM-containing polymers were prepared with a hydroxyethyl methacrylate (HEMA) or methoxyethyl acrylate (MEA) backbone and mixed with polyvinylidene fluoride (PVDF). PVDF was included to increase the cohesive property of the adhesive through hydrogen bond (H-bond) formation with the adhesive polymers. SHAM-containing adhesive demonstrated lap shear adhesion strength (S ) greater than 0.9 MPa to glass, metal, and polymeric surfaces. Adhesive formulations with elevated SHAM-content also demonstrated increased adhesive properties with S values reaching as high as 4.8 MPa. Due …

High-Throughput Analysis Of Topographical Cues For The Expansion Of Murine Pluripotent Stem Cells, Abigail Anne Conner, Yuan Yao, Sarah W. Chan, Deepak Jain, Suzanne M. Wong, Evelyn K. Yim, Muhammad Rizwan

Michigan Tech Publications

The expansion of pluripotent stem cells (PSCs) in vitro remains a critical barrier to their use in tissue engineering and regenerative medicine. Biochemical methods for PSC expansion are known to produce heterogeneous cell populations with varying states of pluripotency and are cost-intensive, hindering their clinical translation. Engineering biomaterials to physically control PSC fate offers an alternative approach. Surface or substrate topography is a promising design parameter for engineering biomaterials. Topographical cues have been shown to elicit profound effects on stem cell differentiation and proliferation. Previous reports have shown isotropic substrate topographies to be promising in expanding PSCs. However, the optimal …

Thrombogenicity Of Biodegradable Metals, D. E.J. Anderson, H. H. Le, H. Vu, J. Johnson, J. E. Aslan, J. Goldman, M. T. Hinds

Michigan Tech Publications

Biodegradable metals offer a promising means to ameliorate many of the long-term risks associated with vascular devices made of conventional biostable stent metals. While numerous biodegradable metal alloys have been developed and characterized in animal models, knowledge of their blood reactivity and thrombogenicity remains unknown. Metal hemocompatibility is particularly valuable because current generation drug-eluting stents pose a significant long-term thrombosis risk. In this study, four pure metals, widely used as degradable base materials (Fe, Zn, Mg, and Mo), and three alloys commonly used in cardiovascular devices [NiTi, CoCr, and stainless steel (SS)] were evaluated. This work examined how each of …

Editorial: Mitral Valve Disease: Mechanisms To Therapies, Maria Carmo Nunes, Hoda Hatoum, Alison M. Pouch, Muralidhar Padala

Michigan Tech Publications

No abstract provided.

Aerobic Exercise Improves Depressive Symptoms In The Unilateral 6-Ohda-Lesioned Rat Model Of Parkinson's Disease, Hannah Loughlin, Jacob Jackson, Chloe Looman, Alayna Starll, Jeremy Goldman, Zhiying Shan, Chunxiu Yu

Michigan Tech Publications

Aerobic exercise has been shown to have established benefits on motor function in Parkinson's disease (PD). However, the impact of exercise on depressive symptoms in PD remains unclear. This study aimed to investigate the effects of regular exercise, specifically using a forced running wheel, on both motor performance and the prevalence of depression in a unilateral 6-OHDA-lesioned rat model of PD. The behavioral outcomes of exercise were assessed through the rotarod test (RT), forelimb adjusting step test (FAST), sucrose consumption test (SCT), and novelty sucrose splash test (NSST). Our data revealed evident depressive symptoms in the PD animals, characterized by …

Association Of Bovine Arch Anatomy With Incident Stroke After Transcatheter Aortic Valve Replacement, Gerardo V. Lo Russo, Hasan S. Alarouri, Abdulah Al-Abcha, Brennan Vogl, Abdulah Mahayni, Agata Sularz, Hoda Hatoum, Jeremy Collins, Juan A. Crestanello, Mohamad Alkhouli

Michigan Tech Publications

BACKGROUND: Acute ischemic stroke complicates 2 % to 3 % of transcatheter aortic valve replacements (TAVRs). This study aimed to identify the aortic anatomic correlates in patients after TAVR stroke. METHODS AND RESULTS: This is a single-center, retrospective study of patients who underwent TAVR at the Mayo Clinic between 2012 and 2022. The aortic arch morphology was determined via a manual review of the pre-TAVR computed tomography images. An "a priori" approach was used to select the covariates for the following: (1) the logistic regression model assessing the association between a bovine arch and periprocedural stroke (defined as stroke within …

Low Shear In Short-Term Impacts Endothelial Cell Traction And Alignment In Long-Term, Mohanish Chandurkar, Nikhil Mittal, Shaina P. Royer-Weeden, Steven D. Lehmann, Yeonwoo Rho, Sangyoon J. Han

Michigan Tech Publications

Within the vascular system, endothelial cells (ECs) are exposed to fluid shear stress (FSS), a mechanical force exerted by blood flow that is critical for regulating cellular tension and maintaining vascular homeostasis. The way ECs react to FSS varies significantly; while high, laminar FSS supports vasodilation and suppresses inflammation, low or disturbed FSS can lead to endothelial dysfunction and increase the risk of cardiovascular diseases. Yet, the adaptation of ECs to dynamically varying FSS remains poorly understood. This study focuses on the dynamic responses of ECs to brief periods of low FSS, examining its impact on endothelial traction-a measure of …

Low Shear In Short-Term Impacts Endothelial Cell Traction And Alignment In Long-Term, Mohanish K. Chandurkar, Nikhil Mittal, Shaina P. Royer-Weeden, Steven D. Lehmann, Yeonwoo Rho, Sangyoon J. Han

Michigan Tech Publications

Within the vascular system, endothelial cells (ECs) are exposed to fluid shear stress (FSS), a mechanical force exerted by blood flow that is critical for regulating cellular tension and maintaining vascular homeostasis. The way ECs react to FSS varies significantly; while high, laminar FSS supports vasodilation and suppresses inflammation, low or disturbed FSS can lead to endothelial dysfunction and increase the risk of cardiovascular diseases. Yet, the adaptation of ECs to dynamically varying FSS remains poorly understood. This study focuses on the dynamic responses of ECs to brief periods of low FSS, examining its impact on endothelial traction—a measure of …

Polymerizing Actin Regulates Myosin-Independent Mechanosensing By Modulating Actin Elasticity And Flow Fluctuation, Nikhil Mittal, Etienne Michels, Kathleen Pakenas, Shaina P. Royer-Weeden, Sangyoon J. Han

Michigan Tech Publications

The stiffness of the extracellular matrix induces differential tension within integrin-based adhesions. However, it has been unclear if the stiffness-dependent differential tension is induced solely by myosin activity. Here, we report that in the absence of myosin contractility, 3T3 fibroblasts still transmit stiffness-dependent differential levels of traction. This myosin-independent differential traction is regulated by polymerizing actin assisted by actin nucleators Arp2/3 and formin where formin has stronger contribution than Arp2/3. Interestingly, we report a four-fold reduction in traction of cells when both Arp2/3 and myosin were inhibited, compared to cells with only myosin inhibition, while there was only a slight …

Myosin-Independent Stiffness Sensing By Fibroblasts Is Regulated By The Viscoelasticity Of Flowing Actin, Nikhil Mittal, Etienne B. Michels, Andrew E. Massey, Yunxiu Qiu, Shaina P. Royer-Weeden, Bryan R. Smith, Alexander X. Cartagena-Rivera, Sangyoon J. Han

Michigan Tech Publications

The stiffness of the extracellular matrix induces differential tension within integrin-based adhesions, triggering differential mechanoresponses. However, it has been unclear if the stiffness-dependent differential tension is induced solely by myosin activity. Here, we report that in the absence of myosin contractility, 3T3 fibroblasts still transmit stiffness-dependent differential levels of traction. This myosin-independent differential traction is regulated by polymerizing actin assisted by actin nucleators Arp2/3 and formin where formin has a stronger contribution than Arp2/3 to both traction and actin flow. Intriguingly, despite only slight changes in F-actin flow speed observed in cells with the combined inhibition of Arp2/3 and myosin …

Modular Open-Source Design Of Pyrolysis Reactor Monitoring And Control Electronics, Finn K. Hafting, Daniel G. Kulas, Etienne Michels, Sarvada Chipkar, Stefan Wisniewski, David Shonnard, Joshua M. Pearce

Michigan Tech Publications

Industrial pilot projects often rely on proprietary and expensive electronic hardware to control and monitor experiments. This raises costs and retards innovation. Open-source hardware tools exist for implementing these processes individually; however, they are not easily integrated with other designs. The Broadly Reconfigurable and Expandable Automation Device (BREAD) is a framework that provides many open-source devices which can be connected to create more complex data acquisition and control systems. This article explores the feasibility of using BREAD plug-and-play open hardware to quickly design and test monitoring and control electronics for an industrial materials processing prototype pyrolysis reactor. Generally, pilot-scale pyrolysis …

Perfusability And Immunogenicity Of Implantable Pre-Vascularized Tissues Recapitulating Features Of Native Capillary Network, Dhavan Sharma, Archita Sharma, Linghao Hu, Te An Chen, Sarah Voon, Kayla J. Bayless, Jeremy Goldman, Alex J. Walsh, Feng Zhao

Michigan Tech Publications

Vascularization is a key pre-requisite to engineered anatomical scale three dimensional (3-D) constructs to ensure their nutrient and oxygen supply upon implantation. Presently, engineered pre-vascularized 3-D tissues are limited to only micro-scale hydrogels, which meet neither the anatomical scale needs nor the complexity of natural extracellular matrix (ECM) environments. Anatomical scale perfusable constructs are critically needed for translational applications. To overcome this challenge, we previously developed pre-vascularized ECM sheets with long and oriented dense microvascular networks. The present study further evaluated the patency, perfusability and innate immune response toward these pre-vascularized constructs. Macrophage-co-cultured pre-vascularized constructs were evaluated in vitro to …

Differential Impact Of Blood Pressure Control Targets On Epicardial Coronary Flow After Transcatheter Aortic Valve Replacement, Brennan Vogl, Alejandra Chavez-Ponce, Adam Wentworth, Eric Erie, Pradeep Yadav, Vinod H. Thourani, Lakshmi Prasad Dasi, Brian Lindman, Mohamad Alkhouli, Hoda Hatoum

Michigan Tech Publications

Background: The cause for the association between increased cardiovascular mortality rates and lower blood pressure (BP) after aortic valve replacement (AVR) is unclear. This study aims to assess how the epicardial coronary flow (ECF) after AVR varies as BP levels are changed in the presence of a right coronary lesion. Methods: The hemodynamics of a 3D printed aortic root model with a SAPIEN 3 26 deployed were evaluated in an in vitro left heart simulator under a range of varying systolic blood pressure (SBP) and diastolic blood pressure (DBP). ECF and the flow ratio index were calculated. Flow index value …

S-Net: A Multiple Cross Aggregation Convolutional Architecture For Automatic Segmentation Of Small/Thin Structures For Cardiovascular Applications, Nan Mu, Zonghan Lyu, Mostafa Rezaeitaleshmahalleh, Cassie Bonifas, Jordan Gosnell, Marcus Haw, Joseph Vettukattil, Jingfeng Jiang

Michigan Tech Publications

With the success of U-Net or its variants in automatic medical image segmentation, building a fully convolutional network (FCN) based on an encoder-decoder structure has become an effective end-to-end learning approach. However, the intrinsic property of FCNs is that as the encoder deepens, higher-level features are learned, and the receptive field size of the network increases, which results in unsatisfactory performance for detecting low-level small/thin structures such as atrial walls and small arteries. To address this issue, we propose to keep the different encoding layer features at their original sizes to constrain the receptive field from increasing as the network …

Bioinspired Materials For Underwater Adhesion With Pathways To Switchability, Chanhong Lee, Huiqi Shi, Jiyoung Jung, Bowen Zheng, Kan Wang, Ravi Tutika, Rong Long, Bruce Lee, Grace X. Gu, Michael D. Bartlett

Michigan Tech Publications

Strong adherence to underwater or wet surfaces for applications like tissue adhesion and underwater robotics is a significant challenge. This is especially apparent when switchable adhesion is required that demands rapid attachment, high adhesive capacity, and easy release. Nature displays a spectrum of permanent to reversible attachment from organisms ranging from the mussel to the octopus, providing inspiration for underwater adhesion design that has yet to be fully leveraged in synthetic systems. Here, we review the challenges and opportunities for creating underwater adhesives with a pathway to switchability. We discuss key material, geometric, modeling, and design tools necessary to achieve …

The Effect Of Slow Strain Rate Tension And Cyclic Loading On Biodegradable Zn–2%Fe–0.8%Mn Alloy In A Simulated Physiological Environment, Lital Ben Tzion-Mottye, Adi Bahar, Tomer Ron, Galit Katarivas Levy, Jeremy Goldman, Dan Eliezer, Eli Aghion

Michigan Tech Publications

Zinc-based alloys have gained increased interest as biodegradable structural materials for medical applications due to their adequate biocompatibility, crucial roles in many physiological functions and attractive antibacterial properties. However, the major drawbacks of zinc alloys relate to their inadequate mechanical properties and tendency to provoke fibrous encapsulation due to relatively high standard potential. Based on the promising effect of Mn on properties of Zn-based alloys, the present study aimed at evaluating the suitability of Zn–2%Fe–0.8%Mn alloy as a potential biodegradable implant under in-vitro conditions. This evaluation focused on the passivation characteristics as determined by cyclic potentiodynamic polarization analysis, immersion test, …

Magnesium-Based Nanocomposites: A Review From Mechanical, Creep And Fatigue Properties, S. Abazari, A. Shamsipur, H. R. Bakhsheshi-Rad, J. W. Drelich, J. Goldman, S. Sharif, A. F. Ismail, M. Razzaghi

Michigan Tech Publications

The addition of nanoscale additions to magnesium (Mg) based alloys can boost mechanical characteristics without noticeably decreasing ductility. Since Mg is the lightest structural material, the Mg-based nanocomposites (NCs) with improved mechanical properties are appealing materials for lightweight structural applications. In contrast to conventional Mg-based composites, the incorporation of nano-sized reinforcing particles noticeably boosts the strength of Mg-based nanocomposites without significantly reducing the formability. The present article reviews Mg-based metal matrix nanocomposites (MMNCs) with metallic and ceramic additions, fabricated via both solid-based (sintering and powder metallurgy) and liquid-based (disintegrated melt deposition) technologies. It also reviews strengthening models and mechanisms that …

Conventional Platinum Metal Implants Provoke Restenosis Responses In Atherogenic But Not Healthy Arteries, Lea M. Morath, Roger J. Guillory Ii, Alexander A. Oliver, Shu Q. Liu, Martin L. Bocks, Galit Katarivas Levy, Jaroslaw Drelich, Jeremy Goldman

Michigan Tech Publications, Part 1

Platinum-containing stents are commonly used in humans with hypercholesterolemia, whereas preclinical stent evaluation has commonly been performed in healthy animal models, providing inadequate information about stent performance under hypercholesterolemic conditions. In this investigation, we used an ApoE−/− mouse model to test the impact of hypercholesterolemia on neointima formation on platinum-containing implants. We implanted 125 μm diameter platinum wires into the abdominal aortas of ApoE−/− and ApoE+/+ mice for 6 months, followed by histological and immunofluorescence examination of neointimal size and composition. It was found that ApoE−/− mice developed neointimas with four times larger area and ten times greater thickness than …

Biodegradable Magnesium Materials Regulate Ros-Rns Balance In Pro-Inflammatory Macrophage Environment, Maria Paula Kwesiga, Amani A. Gillette, Seyedehfatemeh Razaviamri, Margaret E. Plank, Alexia L. Canull, Zachary Alesch, Weilue He, Bruce Lee, Roger J. Guillory Ii

Michigan Tech Publications, Part 1

The relationship between reactive oxygen and nitrogen species (ROS-RNS) secretion and the concomitant biocorrosion of degradable magnesium (Mg) materials is poorly understood. We found that Mg foils implanted short term in vivo (24 h) displayed large amounts of proinflammatory F4/80+/iNOS + macrophages at the interface. We sought to investigate the interplay between biodegrading Mg materials (98.6% Mg, AZ31 & AZ61) and macrophages (RAW 264.7) stimulated with lipopolysaccharide (RAW 264.7LPS) to induce ROS-RNS secretion. To test how these proinflammatory ROS-RNS secreting cells interact with Mg corrosion in vitro, Mg and AZ61 discs were suspended approximately 2 mm above a monolayer of …

Mechanical Properties And Morphological Alterations In Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes, James Dolgin, Samerender Nagam Hanumantharao, Stephen Farias, Carl G. Simon, Smitha Rao

Michigan Tech Publications, Part 1

Electrospinning is a versatile tool used to produce highly customizable nonwoven nanofiber mats of various fiber diameters, pore sizes, and alignment. It is possible to create electrospun mats from synthetic polymers, biobased polymers, and combinations thereof. The post-processing of the end products can occur in many ways, such as cross-linking, enzyme linking, and thermal curing, to achieve enhanced chemical and physical properties. Such multi-factor tunability is very promising in applications such as tissue engineering, 3D organs/organoids, and cell differentiation. While the established methods involve the use of soluble small molecules, growth factors, stereolithography, and micro-patterning, electrospinning involves an inexpensive, labor …

Anti-Inflammatory And Anti-Thrombogenic Properties Of Arterial Elastic Laminae, Jeremy Goldman, Shu Q. Liu, Brandon J. Tefft

Michigan Tech Publications, Part 1

Elastic laminae, an elastin-based, layered extracellular matrix structure in the media of arteries, can inhibit leukocyte adhesion and vascular smooth muscle cell proliferation and migration, exhibiting anti-inflammatory and anti-thrombogenic properties. These properties prevent inflammatory and thrombogenic activities in the arterial media, constituting a mechanism for the maintenance of the structural integrity of the arterial wall in vascular disorders. The biological basis for these properties is the elastin-induced activation of inhibitory signaling pathways, involving the inhibitory cell receptor signal regulatory protein α (SIRPα) and Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP1). The activation of these molecules causes deactivation of …

Can We Explain Machine Learning-Based Prediction For Rupture Status Assessments Of Intracranial Aneurysms?, Nan Mu, M. Rezaeitaleshmahalleh, Z. Lyu, M. Wang, J. Tang, C. M. Strother, J. J. Gemmete, A. S. Pandey, J. Jiang

Michigan Tech Publications, Part 1

Although applying machine learning (ML) algorithms to rupture status assessment of intracranial aneurysms (IA) has yielded promising results, the opaqueness of some ML methods has limited their clinical translation. We presented the first explainability comparison of six commonly used ML algorithms: multivariate logistic regression (LR), support vector machine (SVM), random forest (RF), extreme gradient boosting (XGBoost), multi-layer perceptron neural network (MLPNN), and Bayesian additive regression trees (BART). A total of 112 IAs with known rupture status were selected for this study. The ML-based classification used two anatomical features, nine hemodynamic parameters, and thirteen morphologic variables. We utilized permutation feature importance, …

Fibroblast-Generated Extracellular Matrix Guides Anastomosis During Wound Healing In An Engineered Lymphatic Skin Flap, Alvis Chiu, Wenkai Jia, Yumeng Sun, Jeremy Goldman, Feng Zhao

Michigan Tech Publications, Part 1

A healthy lymphatic system is required to return excess interstitial fluid back to the venous circulation. However, up to 49% of breast cancer survivors eventually develop breast cancer-related lymphedema due to lymphatic injuries from lymph node dissections or biopsies performed to treat cancer. While early-stage lymphedema can be ameliorated by manual lymph drainage, no cure exists for late-stage lymphedema when lymph vessels become completely dysfunctional. A viable late-stage treatment is the autotransplantation of functional lymphatic vessels. Here we report on a novel engineered lymphatic flap that may eventually replace the skin flaps used in vascularized lymph vessel transfers. The engineered …

An Attention Residual U-Net With Differential Preprocessing And Geometric Postprocessing: Learning How To Segment Vasculature Including Intracranial Aneurysms, Nan Mu, Zonghan Lyu, Mostafa Rezaeitaleshmahalleh, Jinshan Tang, Jingfeng Jiang

Michigan Tech Publications, Part 1

Objective

Intracranial aneurysms (IA) are lethal, with high morbidity and mortality rates. Reliable, rapid, and accurate segmentation of IAs and their adjacent vasculature from medical imaging data is important to improve the clinical management of patients with IAs. However, due to the blurred boundaries and complex structure of IAs and overlapping with brain tissue or other cerebral arteries, image segmentation of IAs remains challenging. This study aimed to develop an attention residual U-Net (ARU-Net) architecture with differential preprocessing and geometric postprocessing for automatic segmentation of IAs and their adjacent arteries in conjunction with 3D rotational angiography (3DRA) images.

Methods

The …

An Assessment Of Blood Vessel Remodeling Of Nanofibrous Poly(Ε-Caprolactone) Vascular Grafts In A Rat Animal Model, Jana Horakova, Tereza Blassova, Zbynek Tonar, Connor Mccarthy, Katerina Strnadova, David Lukas, Petr Mikes, Patrick Bowen, Roger J. Guillory Ii, Megan C. Frost, Jeremy Goldman

Michigan Tech Publications, Part 1

The development of an ideal vascular prosthesis represents an important challenge in terms of the treatment of cardiovascular diseases with respect to which new materials are being considered that have produced promising results following testing in animal models. This study focuses on nanofibrous polycaprolactone-based grafts assessed by means of histological techniques 10 days and 6 months following suturing as a replacement for the rat aorta. A novel stereological approach for the assessment of cellular distribution within the graft thickness was developed. The cellularization of the thickness of the graft was found to be homogeneous after 10 days and to have …

Conductive 3d Nano-Biohybrid Systems Based On Densified Carbon Nanotube Forests And Living Cells, Roya Bagheri, Alicia K. Ball, Masoud Kasraie, Aparna Chandra, Xinqian Chen, Ibrahim Miskioglu, Zhiying Shan, Parisa Pour Shahid Saeed Abadi

Michigan Tech Publications

Conductive biohybrid cell-material systems have applications in bioelectronics and biorobotics. To date, conductive scaffolds are limited to those with low electrical conductivity or 2D sheets. Here, 3D biohybrid conductive systems are developed using fibroblasts or cardiomyocytes integrated with carbon nanotube (CNT) forests that are densified due to interactions with a gelatin coating. CNT forest scaffolds with a height range of 120–240 µm and an average electrical conductivity of 0.6 S/cm are developed and shown to be cytocompatible as evidenced from greater than 89% viability measured by live-dead assay on both cells on day 1. The cells spread on top and …

Non-Antibiotic Antimicrobial Polydopamine Surface Coating To Prevent Stable Biofilm Formation On Satellite Telemetry Tags Used In Cetacean Conservation Applications, Ariana Smies, Jeremy Wales, Maureen Hennenfent, Laura Lyons, Caleigh R. Dunn, Jooke Robbins, Bruce Lee, Alexandre Zerbini, Rupak Rajachar

Michigan Tech Publications, Part 1

Satellite telemetry tags, used to monitor the migratory behavior of cetaceans, have the potential to be a vehicle for infection due to their invasive nature. Antibiotic coatings have been previously employed to reduce the chances of infection via the formation of a stable biofilm on the surface of the tags. However, increased use of antibiotics has the potential to lead to the development of antibiotic-resistant pathogens. To prevent the formation of antibiotic-resistant pathogens, a polydopamine surface coating that, when exposed to oxygen, releases low doses ( ~ 40-100 µ M ) of hydrogen peroxide over a prolonged period ( > 24 …

Electrochemical Behaviour And Direct Cell Viability Analysis Of Hybrid Implants Made Of Ti-6al-4v Lattices Infiltrated With A Bioabsorbable Zn-Based Alloy, Noa Gabay Bass, Galit Katarivas Levy, Tomer Ron, Razi Vago, Jeremy Goldman, Amnon Shirizly, Eli Aghion

Michigan Tech Publications, Part 1

Biodegradable metals are being developed for biomedical implants or components of implants. Biodegradable zinc-based materials, in particular, have been shown to promote bone regeneration in orthopaedic applications. Here, we investigated the potential of a hybrid Ti-Zn system, comprising a Ti-6Al-4V biostable lattice produced by additive manufacturing (AM) infiltrated by a bioabsorbable Zn-2%Fe alloy, to serve as an osseointegrated implant for dental and orthopaedic applications. The osseointegration of implants can be enhanced by a porous implant structure that facilitates bone ingrowth to achieve superior bonding between the bone tissue and the implant. The hybrid material was evaluated in terms of microstructure …