Biomedical Engineering and Bioengineering Commons^™

Single-Molecule Orientation And Localization Microscopy, Sophie Brasselet, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Single-molecule localization microscopy (SMLM) offers enhanced spatial resolution in optical microscopy, providing detailed insights into the spatial organization of proteins in cells at the nanoscale. Over the past decade, SMLM has progressively incorporated the capability to retrieve the orientations of single molecules using their polarized dipolar emission pattern. Here we explore recent advancements in single-molecule orientation and localization microscopy (SMOLM), which yields super-resolved images of molecular three-dimensional (3D) orientations, wobble and 3D positions. This advancement opens possibilities to explore the nanoscale organization and conformation of biological molecules as well as to monitor and design local 3D optical fields in nanophotonics. …

Mitigating Melanin-Induced Bias In Pulse Oximetry: Optical, Algorithmic, Engineering, Hardware And Modeling Tools, Mckenzie Bradley, Sydnee Barrett, Ty Mckelvey, Jeremiah Carpenter, Delphine Dean

Publications

Melanin, the primary determinant of skin pigmentation, absorbs light at wavelengths that can have significant impact on the accuracy of pulse oximetry and other optical biosensing methods. This narrative review examines key factors influencing melanin-dependent pulse oximetry inaccuracies, including optical interference in transmission and reflectance modes. These inaccuracies further highlight the need for use of standardized skin tone metrics in device testing and design such as the Monk Skin Tone scale and Individual Typology Angle for performance stratification. There are several approaches in development that hope to address the errors in pulse oximetry measurements on melanin-rich skin. These include algorithmic …

Measuring Neck Morphologies Using Upright Magnetic Resonance Imaging, Narayan Yoganandan, Mahmudur Rahman, Ali Warriach, Karthik Banurekha Devaraj, Brian D. Stemper, Aditya Vedantam, Christopher Wolfla, Peter Le

Biomedical Engineering Faculty Research and Publications

Introduction

Because the head supported mass worn by military pilots adds loading to the head-neck complex, morphometrical characteristics of neck muscles are expected to be different from those that do not wear the added mass. To accurately determine segmental neck loads using computational whole-body human or head-neck models, it is important to include accurate morphometrical properties of cervical muscles from the population of interest, in this case military pilots. Routine radiographic imaging (x-rays and computed tomography scans) is less optimal, while magnetic resonance imaging (MRI) is appropriate. Although MRI scanners are generally available in clinical settings, recumbent scanning does not …

Comprehensive Review Of In Vitro Approaches For Environmental Heavy Metal Exposure, Manas Warke, Madeline English, Camila Padilla, Lexie Gasco, Wendy Leisner, Rupali Datta, Smitha Rao

Michigan Tech Publications

Heavy metals are ubiquitous environmental pollutants, contaminating air, soil, and water via the erosion of natural deposits, as well as originating from anthropogenic sources, such as agriculture, industries, transportation, and landfills. The increasing utilization of heavy metals over the years, combined with the persistent nature of metals in the environment poses a direct threat to human and environment health. Although regulatory limits have been established for toxic metals, assessing the associated health risks using real-life exposure scenarios remains challenging. In this review, we summarize the development and use of in vitro models based two- and three-dimensional cell culture systems, focusing …

Tissue Engineered Combinatorial Therapeutics For Spinal Cord Injury Repair, Inha Baek

Graduate Theses and Dissertations

Traumatic spinal cord injury (SCI) lead to temporary or permanent sensorimotor deficit due to the complex and multifaceted features of the injury site, making the treatments ineffective. Recent research emphasizes the potential of combinatorial therapeutics approaches combining different therapeutics, such as biomaterials and stem cell transplantation. In this context, nerve composite hydrogels, fabricated from decellularized sciatic nerve (dSN) and spinal cord (dSC) extracellular matrices (ECM), might be promising platforms due to their biocompatibility and ability to mimic native microenvironments. In this study, we developed and characterized nerve mimetic composite hydrogels embedded with human adipose-derived stem cells (hASCs) and investigated their …

Lung Injury Risk Curves From Behind Armor Blunt Trauma Using A Live Swine Model, Narayan Yoganandan, Lewis Somberg, Danielle Wilson, Alok Shah, Jared Michael Koser, Brian D. Stemper, Valeta Carol Chancey, Joseph Mcentire

Biomedical Engineering Faculty Research and Publications

Introduction

From structural, anatomical, and functional perspectives, components of the thoracoabdominal region are heterogeneous and physiologically and functionally different. Although their tolerances to injury are expected to be different, the current Roma Plastilina No. 1 clay penetration criterion for behind armor blunt trauma (BABT) is not specific to the body region. It is important to develop regional injury criteria to ensure its specificity. The objective of the study is to conduct impact tests on the lung region using a live animal model and develop injury risk curves using velocity and deflection metrics via parametric survival analysis.

Materials and Methods

Live …

Low-Cost Cutaneous Protoporphyrin Ix (Ppix) Detection (Cpd) Device For Follow-Up Monitoring Of Patients After Photodynamic Therapy, Md Asaduzzaman Rasel

Graduate Masters Theses

Background: Photodynamic Therapy (PDT) utilizes specific wavelengths of light to activate photosensitizing chemical compounds, known as photosensitizers, which induce the generation of cytotoxic reactive oxygen species (ROS) for the targeted destruction of cancer cells. Among various photosensitizers for PDT, Protoporphyrin IX (PpIX) is widely employed in oncology and dermatology due to its natural in situ generation via the metabolic conversion of 5- aminolevulinic acid (ALA), a non-phototoxic prodrug. Systemic administration of ALA after 3-6 hr drug delay leads to peak PpIX accumulation in tissues, facilitating therapeutic and diagnostic applications. However, PpIX can persist in the skin for 24–48 hours post-treatment, …

Design And Development Of A Standalone Digital Holographic Microscope Employing Phase-Driven Reconstruction And Classification For Biomedical Imaging And Optical Diagnostics, Charlotte Kyeremah

Graduate Doctoral Dissertations

Access to advanced biomedical imaging technologies remains a significant challenge in resource-limited settings, especially for early disease detection and monitoring of diseases such as malaria, HIV, and other blood-borne diseases. Although point-of-care (POC) devices have gained popularity in global health, many rely on antibody-based tests, lateral flow strips, or optical readouts that often lack quantitative capabilities, sensitivity to early infections, or versatility in different diagnostic targets. In addition, these systems are typically dependent on disposable reagents or manual interpretation, which limits their effectiveness in remote areas. Digital Holographic Microscopy (DHM) presents a promising alternative as a label-free imaging method capable …

Predicting The Response Of Tendon-Driven Prosthetic Finger With Hyperelastic Joints, Lucas Gallup, Mohamed Trabia, Brendan O'Toole

Mechanical Engineering Faculty Research

Properly designed prosthetics hands can enhance the quality of life for those suffering from limb loss. Recently, 3D-printed prosthetic hands are becoming common. In these prostheses, fingers flex through the tendons that are activated by motion of the wrist. To provide spring action, thermoplastic polyurethane (TPU) hyperelastic joints are used to connect digits to each other as well as to the wrist. While these designs are common, no model for the relationship between tendon tension and joint flexion is available. This work has developed a quasi-static virtual work-based model to predict the relationship between tendon forces and the flexion of …

Sar Analog-To-Digital Converters For Point-Of-Care Biosensors: A Comparison, Eithar Alammari, Joud Alamro, Sara Alashwali, Ghadah S. Alyami, Aziza I. Hussein

Effat Undergraduate Research Journal

The SAR ADC, recognized for its low power consumption, moderate resolution, and satisfactory processing speed, stands as an ideal choice for ultra-low power biomedical applications. Recent efforts have been concentrated on enhancing the power efficiency of the SAR ADC sub-components through intricate refinements and innovative techniques. These efforts aim to minimize energy consumption without compromising the ADC's performance. Therefore, this paper aims to thoroughly examine various implementation approaches for the main components of the SAR ADC, highlighting their individual strengths, weaknesses, and limitations within wireless biomedical applications.

Developing Deep Learning Methods For Cognitive Impairment Detection Based On Non-Invasive Data, Muath Alsuhaibani

Electronic Theses and Dissertations

Cognitive impairment detection is on the rise to help reduce the burden of healthcare costs on institutions and individuals. Mild Cognitive Impairment (MCI) is an early stage of cognitive decline progressing to Alzheimer’s disease (AD) or AD-related Dementia (ADRD). Detecting the early stages of AD/ADRD is crucial for early interventions among older adults to mitigate cognitive decline over time. However, the current diagnostic methods are often costly and/or invasive, such as MRI and PET scans. Thus, the search for non-invasive and cost-effective screening tools for the early detection of cognitive impairment using speech, language, visual, and motor data is growing. …

Surgeon Ergonomics While Performing Total Knee Arthroplasty, Cameron Dahman

Electronic Theses and Dissertations

One of the most prevalent hazards to orthopedic surgeons are musculoskeletal disorders with 97% of arthroplasty surgeons reporting procedural-related musculoskeletal pain and 66% of orthopedic surgeons reporting procedural-related musculoskeletal injury [1], [2], [3]. With the annual number of total knee arthroplasties (TKA) on the rise, orthopedic surgeons are taking on larger annual caseloads and retiring later in life [4], [5]. The goal of this study was to quantify postural demand for 11 discrete surgical steps of TKA to identify ergonomically unfavorable positions surgeons experience while operating. Four orthopedic surgeons performed TKA while their motions were recorded using marker-based motion capture. …

Influence Of Obesity On Knee Joint Mechanics: A Subject-Specific Musculoskeletal Modeling Approach, Ryan C. Knowles

Electronic Theses and Dissertations

Obesity has been consistently linked to knee osteoarthritis (KOA), a debilitating joint disease. While prior work has shown that obese individuals often walk with altered biomechanics compared to normal-weight (NW) individuals, which may accelerate KOA progression, less is known about how these mechanics affect load distribution between the medial and lateral tibiofemoral (TF) compartments, particularly during higher-demand activities. This study developed subject-specific musculoskeletal models of five obese and five NW individuals, incorporating CT-based bone measurements and individualized joint contact points. Simulations were driven by six degree-of-freedom (6DOF) kinematics obtained from high-speed stereo radiography (HSSR) to predict TF loading and associated …

Investigation Of A Divergent Triangle Construct For Internal Fixation Of Vertical Femoral Neck Fractures Using Experimental And Finite Element Analysis Methods, Hannah Rainey

Masters Theses

Hip fractures are a significant public health issue as they are a commonly encountered problem and have a high rate of mortality. The fracture of the femoral neck is difficult to treat due to the geometry of the proximal femur and the weight-bearing capacity that the structure must comply with. Internal fixation of the femoral neck is often accomplished with three screws inserted along the axis of the femoral neck. The optimal configuration and number of these screws is not agreed upon in the literature. The purpose of this study was to conduct a pilot investigation on the biomechanical performance …

Ceramic Waste As Sustainable Material In Self-Compacting Concrete: Experimental Study And Ann Predictive Modeling, Abderrezak Bouziane, Houssam Slimanou, Mohamed Amin Bouzidi, Nedjima Bouzidi

Journal of Sustainable Construction Materials and Technologies

Recycling of ceramic waste in the construction sector is a promising option for reducing the environmental impacts of the concrete industry. The main objective of this study is the elaboration of a solid predictive model for ceramic waste based self-compacting (SCC) concrete properties, using artificial neural networks (ANN). This further, reduces its environmental impact without compromising satisfactory performance. An experimental approach was adopted with different substitution ratios: 0 to 30 wt% and particle sizes ranging from 3-8 mm and 8-15 mm. Tested properties of modified SCC as compressive strength at 7 and 28 days (fc7, fc28), spread (L), sieve stability …

Comparative Study On The Performance Of Ggbs, Uggbs, And Rha-Incorporated Cement Mortar Mixes, Devansh Goel, Sudarshan D. Kore

Journal of Sustainable Construction Materials and Technologies

The environmental footprint of cement production has accelerated the pursuit of sustainable alternatives in the construction industry. Supplementary cementitious materials (SCMs), particularly those derived from industrial and agricultural waste, offer a promising approach to reducing cement consumption while maintaining performance. This study evaluates the fresh and mechanical behavior of binary, ternary, and quaternary cementitious mortar blends incorporating ground granulated blast-furnace slag (GGBS), ultrafine GGBS (UGGBS), and rice husk ash (RHA) as partial replacements for cement. A total of 32 mortar mixes were designed with water-to-binder (w/b) ratios ranging from 0.3 to 0.5 and varied replacement levels of RHA (0-20%), GGBS …

Correlations Between Hemodynamics And Radiomic Features In Thrombosed Intracranial Aneurysms, Zonghan Lyu, Andres Gudino, Navami Shenoy, Carlos Dier, Elena Sagues, Jingfeng Jiang, Edgar A. Samaniego

Michigan Tech Publications

Purpose: Evaluating intracranial aneurysm (IA) rupture risk is essential for guiding management. Although intrasaccular thrombosis (IST) is less common, it can contribute to aneurysm growth, mass effect, and rupture. Aneurysm wall enhancement (AWE) on high-resolution MRI (HR-MRI) offers valuable insight into IST and IA progression. Using radiomics, we extracted spatial information of the aneurysm wall to characterize AWE. This study aimed to explore correlations between radiomics-based AWE profiles and gross hemodynamic parameters, integrating imaging and flow dynamics to better understand IST. Methods: Radiomic analysis was conducted on a cohort of 3T HR-MRI scans from IA with IST. Three-dimensional vascular reconstructions …

Impact Analysis On Silicone Breast Implants Using In Vitro Experiments And Finite Element Analysis, Brenna Marie Tiernan

Masters Theses

Silicone breast implants are commonly used in breast augmentation and reconstruction procedures. Mentor introduced two newer models of silicone gel-filled implants, the MemoryGel^TM Xtra and MemoryGel^TM Boost, designed to increase the gel fill and projection of the implants while reducing wrinkling and rippling of the shell. Modifications to the shape and fill of these implants may result in different mechanical responses under compressive or impact loading conditions. To investigate whether these structural differences influence implant behavior, in vitro drop testing and finite element analysis (FEA) simulations were conducted, focusing on impact forces, internal stresses, and deformation patterns experienced …

Palpation Characteristics Of An Instrumented Virtual Cricothyroidotomy Simulator, Melih Turkseven, Trudi Di Qi, Ganesh Sankaranarayanan, Suvranu De

Engineering Faculty Articles and Research

Cricothyroidotomy (CCT) is a critical, life-saving procedure requiring the identification of key neck landmarks through palpation. Interactive virtual simulation offers a promising, cost-effective approach to CCT training with high visual realism. However, developing the palpation skills necessary for CCT requires a haptic interface with tactile sensitivity comparable to human fingers. Such interfaces are often represented by plastic partial mannequins, which require further adaptation to integrate into virtual environments. This study introduces an instrumented physical palpation interface for CCT, integrated into a virtual surgical simulator, and tested on 10 surgeons who practiced the procedure over a training period. Data on haptic …

Facile Fabrication Of Protein-Polysaccharide Conjugate Multifilament Nerve Guidance Conduit For Peripheral Nerve Regeneration, Preethy Amruthavarshini R

Theses and Dissertations

Long segmental defects in peripheral nerves impair sensory and motor function by disrupting neural impulse transmission. End-to-end autologous grafting with proper fascicular complementation has demonstrated functional recovery per the Medical Research Council Classification (MRCC). However, obtaining donor nerve tissue exceeding 30 mm remains a significant challenge. Artificial hollow conduits serve as an alternative but are limited to defects smaller than 30 mm due to inadequate innervation across the lumen.

Multichannel nerve guidance conduits (mNGCs) have emerged as a promising solution by enhancing fascicular complementation similar to autografts. This study presents a two-step approach for fabricating nerve conduits with precise fascicular …

Functional Biopolymers Applied To Sustainable Technologies In The Environment And Healthcare, Fengjie He

UNLV Theses, Dissertations, Professional Papers, and Capstones

Biodegradable polymeric materials (biopolymers) are naturally derived materials known for their excellent biocompatibility, biodegradability, sustainability, and versatile chemical functionality. They have attracted increasing attention in various applications as alternative to synthetic materials ranging from food packaging to tissue engineering. Meanwhile, with intrinsic advantages, biopolymers have also emerged as promising materials in addressing contemporary challenges in both biomedical and environmental fields. Motivated by the significant potential of biopolymers and the growing need for sustainable materials, my research focuses on the design and engineering of biodegradable polymers with novel modification methods and application directions. In this work, two representative biopolymers are selected: …

Validity Of Body Composition Estimates In Women Assessed By A Multifrequency Bioelectrical Impedance Device, Mitchell Evan Zaplatosch, Juliana F. Meireles, Janeen S. Amason, Sadaf Dabeer, Brian M. Kliszczewicz, Gerald Mangine, Valene G. Barry, Barbara A. Gower, Katherine H. Ingram

Faculty Articles

Background: Multifrequency bioelectrical impedance devices such as the InBody 770 (IB770) offer faster measurements and lower costs compared with other body composition assessments. This study validated measures from IB770 against the deuterium oxide dilution technique (D2O) and DXA and compared a four-compartment (4C) model using total body water (TBW) derived from IB770 compared with D2O. Methods: A total of 55 adult females (mean ± SD, age: 21.1 ± 2.6 years) completed IB770 and DXA scans and the D2O protocol. Lin’s concordance correlation coefficients (CCCs), Bland–Altman analyses, and other equivalence tests evaluated agreement between IB770 and the criterion for measures of …

Effect Of Arm Movement On Lower Body Muscle Contributions To Standing Long Jump Performance, Abigail Douglas

Masters Theses

Previous jumping studies have proven that jumping with arm motion enhances performance compared to jumping without arm motion. The purpose of this study was to investigate the effect of arm motion on the muscle contributions of the lower extremities during the standing long jump using muscle-actuated modeling informed by experimental data. Specifically investigating how arm motion enhances performance at the muscle level and how the muscle contributions of the lower extremities differ in jumps with and without arm motion. Three male and three female participants performed three jumps with arms and three jumps without arms. The longest jumps with and …

Fourier-Feature Mlp Toolkit For Gpu-Accelerated Cardiac-Mri 4dcmr Strain Analysis, Aarnav T. Sabale, Marco A. Prado, Craig J. Goergen

Discovery Undergraduate Interdisciplinary Research Internship

This paper explores the embedding of a Fourier-Feature—enhanced multiplayer perceptron(MLP-FEE) at the heart of a newly refactored python workflow for four-dimensional cardiac-MRI strain quantification demonstrating how a single, compact network can outperform traditional convolution and spline-based methods. The original code, capable of orientation normalization, displacement tracking, and finite-difference strain computation, has been translated and consolidated into pytorch. By injecting sinusoidal positional encodings at the network’s input layer supplied a rich set of high-frequency basis functions hence enabling multilayer MLP to resolve gradients that cubic splines and conventional CNNs typically blur or struggle with. Profiling on an Apple-silicon GPU shows interactive …

Advancing Single-Molecule Nanopore Sensing: From Protein Conformation And Metal Binding To Dna Transport Via Hybrid Nanostructures, Matthew J. Odonohue

Multidisciplinary Studies Theses and Dissertations

This dissertation presents the development and application of solid-state and hybrid nanopore platforms for single-molecule analysis of biomolecular interactions. First, a silicon nitride nanopore was employed to characterize the conformational states of the transferrin receptor (TfR), enabling discrimination between its monomeric and dimeric forms, as well as from its ligand, human serum transferrin (hSTf), despite their similar molecular weights. In a separate study, the same nanopore configuration was used to track the real-time binding of titanium ions to transferrin, demonstrating single-molecule sensitivity to metal-induced conformational changes. A hybrid microtubule–solid-state nanopore (MT–SSN) was then engineered, repurposing microtubules as electrostatically anchored conduits …

Novel Nanomaterials For Developing Bone Scaffolds And Tissue Regeneration, Nazim Uddin Emon, Lu Zhang, Shelby Dawn Osborne, Mark Allen Lanoue, Yan Huang, Z. Ryan Tian

Cell and Molecular Biology Faculty Publications and Presentations

Nanotechnologies bring a rapid paradigm shift in hard and soft bone tissue regeneration (BTR) through unprecedented control over the nanoscale structures and chemistry of biocompatible materials to regenerate the intricate architecture and functional adaptability of bone. This review focuses on the transformative analyses and prospects of current and next-generation nanomaterials in designing bioactive bone scaffolds, emphasizing hierarchical architecture, mechanical resilience, and regenerative precision. Mainly, this review elucidated the innovative findings, new capabilities, unmet challenges, and possible future opportunities associated with biocompatible inorganic ceramics (e.g., phosphates, metallic oxides) and the United States Food and Drug Administration (USFDA) approved synthetic polymers, including …

Utilizing Wearable Technology To Improve Pots Diagnostic Testing, Monique Watson, Damen Wilson, Matthew Ward

Discovery Undergraduate Interdisciplinary Research Internship

Postural orthostatic tachycardia syndrome (POTS) is a form of dysautonomia characterized by a heart rate (HR) increase ≥30 beats/minute (bpm) within 10 minutes of standing from a supine position, without a significant blood pressure drop. POTS can diminish quality of life with symptoms including tachycardia, headaches, and fatigue. Despite established diagnostic criteria, POTS is often underrecognized or misdiagnosed due to its episodic nature. Symptoms may not consistently be present during diagnostic testing, leading to false negatives during in-office testing. Wearables offer a promising solution through continuous physiological monitoring. Individuals with hypermobile Ehler-Danlos Syndrome (hEDS), a population in which POTS is …

Volumetric Imaging Of Cellular Dynamics Using Light-Sheet Microscopy: From Subcellular Structures To Whole Organisms, Md Nasful Huda Prince

Optical Science and Engineering ETDs

Advancing biomedical imaging requires platforms that combine high-resolution, speed, and stability across biological scales. This dissertation presents three innovations designed to address limitations of conventional light-sheet and oblique plane imaging. First, we introduce Reflected Inline Detection in Epi-Oblique Plane Microscopy (RIDE-OPM), a compact and drift-resistant system that eliminates a tertiary detection path while maintaining alignment flexibility, enabling robust long-term live imaging. Second, we present a high-speed Axially Swept Light-Sheet Microscopy (ASLM) platform for cleared tissues, using dual foci and synchronized sweeping to achieve isotropic sub-micron resolution at up to 40 frames-per-second, a fourfold improvement over standard ASLM. Integrated with a …

Measurements Of Spinal Posture During Common Strawberry Picking Positions Using Inertial Measurement Units, Madeline E. Everson

Master's Theses

Physically demanding professions, such as agriculture, put people at an increased

risk for experiencing back pain. This is due to the awkward and harmful postures sustained

throughout their workday. While studies have been done demonstrating that back pain is

prevalent amongst this community, most do not collect motion capture (MoCap) data,

leaving the specifics of the kinematics, such as the amount of time spent in harmful

postures, unknown. Unfortunately, MoCap data is typically limited to that of a lab or

facility where the cameras are housed. Therefore, this study aimed to mitigate the

inaccessibility of traditional MoCap by validating that …

Axicons For Improved Resolution And Depth Of Focus In Adaptive Optics Scanning Light Ophthalmoscopy, Ruth E. Woehlke, Robert F. Cooper

Biomedical Engineering Faculty Research and Publications

Adaptive optics scanning light ophthalmoscopes are instrumental for studying the eye, yet they use truncated Gaussian illumination and are limited by diffraction, restricting resolution and depth of focus (DOF). Non-diffractive Bessel beams have emerged as an alternative. Here, we use two axicons configured as: (1) an extended DOF beam for resolving multiple retinal layers; and (2) an annular beam for increasing lateral resolution. We compare both approaches to truncated Gaussian illumination using a variety of metrics. Using Configuration 1, we successfully observed multiple retinal layers simultaneously. Using Configuration 2, we observed enhanced lateral resolution but decreased signal-to-noise ratio.