Biomedical Engineering and Bioengineering Commons^™

A Method To Assess Adherence In Inhaler Use Through Analysis Of Acoustic Recordings Of Inhaler Events, Shona D’Arcy, Elaine Machale, Jansen Seheult, Martin S. Holmes, Cian Hughes, Imran Sulaiman, Deirdre Hyland, Conor O’Reilly, Senan Glynn, Thekra Al-Zaabi, John Mccourt, Terence Taylor, Frank Keane, Isabelle Killane, Richard B. Reilly, Richard W. Costello

Articles

Rationale: Poor adherence to inhaler use can be due to poor temporal and/or technique adherence. Up until now there has been no way of reliably tracking both these factors in everyday inhaler use. Objectives: This paper introduces a device developed to create time stamped acoustic recordings of an individual’s inhaler use, in which empirical evidence of temporal and technique adherence in inhaler use can be monitored over time. The correlation between clinical outcomes and adherence, as determined by this device, was compared for temporal adherence alone and combined temporal and technique adherence. Findings: The technology was validated by showing that …

Investigation Of Cardiac Electrophysiology In Human Ventricular Tissue, Kathleen Brownson

Theses and Dissertations--Biomedical Engineering

Individuals with cardiomyopathy are at higher risk to die from sudden cardiac arrest than those with non-failing (NF) hearts. This study examined the differences in electrical properties of failing and NF human hearts in terms of cardiac memory through explicit control of diastolic intervals in a sinusoidal fashion, restitution of action potential duration (APD) through standard and dynamic pacing protocols, maximum rate of depolarization and APD alternans. Recordings of transmembrane potentials were made in tissues extracted from patients with heart failure and one donor NF heart. Computational simulations were performed using the O’Hara Rudy model for generating surrogates of control …

Prefrontal Responses To Digit Span Memory Phases In Patients With Post-Traumatic Stress Disorder (Ptsd): A Functional Near Infrared Spectroscopy Study, Fenghua Tian, Amarnath Yennu, Alexa Smith-Osborne, F. Gonzalez-Lima, Carol S. North, Hanli Liu

Bioengineering Faculty Publications - Archive

Neuroimaging studies of post-traumatic stress disorder (PTSD)-related memory impairments have consistently implicated abnormal activities in the frontal and parietal lobes. However, most studies have used block designs and could not dissociate the multiple phases of working memory. In this study, the involvement of the prefrontal cortex in working memory phases was assessed among veterans with PTSD and age-/ gender-matched healthy controls. Multichannel functional near infrared spectroscopy (fNIRS) was utilized to measure prefrontal cortex hemodynamic activations during memory of neutral (i.e., not trauma-related) forward and backward digit span tasks. An event-related experimental design was utilized to dissociate the different phases (i.e., …

The Effects Of Body Armor On Lower Back And Knee Biomechanics During Basic And Military Inspired Tasks, Megan P. Phillips

Theses and Dissertations--Biomedical Engineering

With increased military personal protection equipment, body armor, comes the addition of carried load. Such person protection in recent history has been instrumental in combating the imminent threats (e.g., improvised explosive devices) of hostile environments, preventing otherwise lethal injuries. However, body armor has been suggested to degrade warfighters’ performance and compound the risk of musculoskeletal injuries. Both performance and risk of injury are intensely related to joint biomechanics. Therefore the objective of this project was set to determine the immediate and prolonged effects of wearing body armor on biomechanics of the lower back and knee. A randomized cross-over study design, …

Interpolation-Based Super-Resolution Reconstruction: Effects Of Slice Thickness, Amir Pasha Mahmoudzadeh, Nasser H. Kashou

Biomedical, Industrial & Human Factors Engineering Faculty Publications

Standard clinical magnetic resonance imaging (MRI) is acquired in two-dimensions where the in-plane resolution is higher than the slice select direction. These acquisitions include axial, coronal, and sagittal planes. To date, there have been few attempts to combine the information of these three orthogonal orientations. This paper aims to take advantage of the different in-plane resolution acquired from each plane orientation and combine them into one volume in order to attain a higher resolution image. This combination of MRI data will allow the detection of smaller areas that would otherwise be missed using only one slice orientation. A comparison of …

Biom 4110/6110: The Science Of Medicine (Syllabus), Bradford D. Pendley

Biomedical Engineering Syllabi Archive

Course Description: Integration of fundamental principles from physics, chemistry, biology and mathematics, and applications of these principles to solve problems in medicine.

Dielectric Characterization Of Coastal Cartilage Chondrocytes, Michael W. Stacey, Ahmet C. Sabuncu, Ali Beskok

Bioelectrics Publications

BACKGROUND: Chondrocytes respond to biomechanical and bioelectrochemical stimuli by secreting appropriate extracellular matrix proteins that enable the tissue to withstand the large forces it experiences. Although biomechanical aspects of cartilage are well described, little is known of the bioelectrochemical responses. The focus of this study is to identify bioelectrical characteristics of human costal cartilage cells using dielectric spectroscopy.

METHODS: Dielectric spectroscopy allows non-invasive probing of biological cells. An in house computer program is developed to extract dielectric properties of human costal cartilage cells from raw cell suspension impedance data measured by a microfluidic device. The dielectric properties of chondrocytes are …

Delivery Of Interleukin-15 To B16 Melanoma By Electroporation Leads To Tumor Regression And Long-Term Survival, Bernadette Marrero, Shawna Shirley, Richard Heller

Bioelectrics Publications

Electroporation (EP) is a method used to physically deliver therapeutic molecules such as plasmid DNA directly to tissues. It has been used safely and successfully in clinical studies and preclinical cancer models to deliver genes to a variety of tissues. In cancer research cytokine therapy is emerging as a promising tool that can be used to boost the host response to tumor antigens. The delivery of cytokines as recombinant proteins can result in toxicity and other adverse effects; however the delivery of cytokine genes using EP has been shown to be safe and effective. Interleukin 15 (IL-15) is a cytokine …

Temporal Modulation Of Plasma Species In Atmospheric Dielectric Barrier Discharges, Aijun Yang, Xiaohua Wang, Dingxin Liu, Mingzhe Rong, Michael G. Kong

Bioelectrics Publications

The atmospheric pressure dielectric barrier discharge in helium is a pulsed discharge in nature and the moment of maximum species densities is almost consistent with peak discharge current density. In this paper, a one-dimensional fluid model is used to investigate the temporal structure of plasma species in an atmospheric He-N₂ dielectric barrier discharge (DBD). It is demonstrated that there exist microsecond delays of the moments of the maximum electron and ion densities from the peak of discharge current density. These time delays are caused by a competition between the electron impact and Penning ionizations, modulated by the N_{2 …}

Novel Technologies For The Detection And Mitigation Of Drowsy Driving, Samuel Lawoyin

Theses and Dissertations

In the human control of motor vehicles, there are situations regularly encountered wherein the vehicle operator becomes drowsy and fatigued due to the influence of long work days, long driving hours, or low amounts of sleep. Although various methods are currently proposed to detect drowsiness in the operator, they are either obtrusive, expensive, or otherwise impractical. The method of drowsy driving detection through the collection of Steering Wheel Movement (SWM) signals has become an important measure as it lends itself to accurate, effective, and cost-effective drowsiness detection. In this dissertation, novel technologies for drowsiness detection using Inertial Measurement Units (IMUs) …

Rheological Model Of Force Transmission Through The Helmet And Concussion Sensitivity, Isiah Kendall, Anthony Vicini, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

In contact sports, head-to-head collisions can lead to concussions, which pose serious health risks to players. This research aims to understand the force transfer from the helmet to the brain that causes concussions in collisions using a rheological model. Experimental data was gathered from players in the National Football League and testing of one type of helmet and padding. The rheological model was verified with published data and good correlation was achieved. Further sensitivity analysis of concussion risk was performed with respect to force, body weight, mass, and impact duration fit to normal and Weibull distributions using Monte Carlo simulations …

Chemosensitive Neurons Of The Locus Coeruleus And The Nucleus Tractus Solitarius: Three Dimensional Morphology And Association With The Vasculature, Cathy D. Graham

Browse all Theses and Dissertations

We studied the 3 dimensional structure of LC and cNTS neurons from neonatal rats. Neurons were identified as either chemosensitive or nonchemosensitive based on their firing rate response to hypercapnia, using whole-cell patch clamping and were passively loaded with Lucifer Yellow. The identified, loaded neurons were reconstructed and analyzed using Neurolucida Neuron Tracing Software. Chemosensitive LC neurons have less rounded soma than nonchemosensitive neurons, and they have dendrites arising from two poles to produce a fusiform appearance. The dendritic branches extend a greater distance from the soma of chemosensitive than nonchemosensitive neurons. Conversely, chemosensitive cNTS neurons were found to have …

Guanine Nucleotide Exchange Activity Of Phospholipase D2 And Its Regulation, Madhupriya Mahankali

Browse all Theses and Dissertations

Phospholipase D2 (PLD2) is a cell membrane lipase that catalyzes the break down of phosphatidyl choline (PC) to phosphatidic acid (PA) and choline. PA is one of the major lipid second messengers that modulate a wide variety of signaling pathways, which are crucial for various cellular functions. In addition to this, PLD2 via binding to a large number of proteins and thereby regulates them independent of its lipase activity. Whereas PLD2 is involved in plethora of cellular functions, high levels of protein and/or activity of PLD2 are reported in variety of disease conditions, such as cancer metastasis and chronic inflammation …

Electromyogram Synergy Control Of A Dexterous Artificial Hand To Unscrew And Screw Objects, Benjamin A. Kent, Nareen Karnati, Erik D. Engeberg

Mechanical Engineering Faculty Research

Due to their limited dexterity, it is currently not possible to use a commercially available prosthetic hand to unscrew or screw objects without using elbow and shoulder movements. For these tasks, prosthetic hands function like a wrench, which is unnatural and limits their use in tight working environments. Results from timed rotational tasks with human subjects demonstrate the clinical need for increased dexterity of prosthetic hands, and a clinically viable solution to this problem is presented for an anthropomorphic artificial hand.

Measuring Brain Iron In Healthy Controls And Accessing Venous Abnormalities In Parkinson's Disease Using Mri, Manju Liu

Wayne State University Dissertations

Iron plays a key role in a number of brain cellular processes. However, abnormal brain iron regulation correlates with a number of neurodegenerative diseases such as Parkinson's disease, multiple sclerosis and so forth. To further elucidate the involvement of iron in neurodegenerative disorders, a robust in vivo quantitative non-invasive assessment of normal brain iron deposition over time is essential. A variety of magnetic resonance imaging (MRI) methods have been used to quantify iron both in vivo and in vitro non-invasively. We first created the baseline of phase value and ∆R2^* as a function of age in seven gray matter nuclei. …

A Method To Modify Angle-Stable Intramedullary Nail Construct Compliance, Patrick Atkinson, B. P. Flanagan, D. Lecronier, M. R. Kubacki, P. Telehowski

Mechanical Engineering Publications

Traditional interlocked intramedullary (IM) nails have recently been modified to provide enhanced angular stability. These so-called ‘angle-stable’ IM nails are designed to eliminate construct toggle and also provide increased axial, bending, and torsional stiffness. While this added stability is needed for small fracture gaps to heal, angle-stable nails may be too stiff for large fracture gaps to unite. Even though relative stability is recommended for large fracture gaps, recent in vivo data indicates that traditional nails may allow for too much motion for healing to occur. The current study evaluated a modified technique for implanting an angle-stable nail which allows …

Biomechanical Performance Of Variable And Fixed Angle Locked Volar Plates For The Dorsally Comminuted Distal Radius, Patrick Atkinson, D. Martineau, J. Shorez, C. Beran, A. G. Dass

Mechanical Engineering Publications

Background

The ideal treatment strategy for the dorsally comminuted distal radius fracture continues to evolve. Newer plate designs allow for variable axis screw placement while maintaining the advantages of locked technology. The purpose of this study is to compare the biomechanical properties of one variable axis plate with two traditional locked constructs.

Methods

Simulated fractures were created via a distal 1 cm dorsal wedge osteotomy in radius bone analogs. The analogs were of low stiffness and rigidity to create a worst-case strength condition for the subject radius plates. This fracture-gap model was fixated using one of three different locked volar …

Design And Study Of The Efflux Function Of The Egfp Fused Mexab-Oprm Membrane Transporter In Pseudomonas Aeruginosa Using Spectroscopy, Feng Ding, Kerry J. Lee, Ardeschir Vahedi-Faridi, Hiroshi Yoneyama, Christopher J. Osgood, Xiao-Hong Nancy Xu

Biological Sciences Faculty Publications

Multidrug membrane transporters (efflux pumps) can selectively extrude a variety of structurally and functionally diverse substrates (e.g., chemotoxics, antibiotics), leading to multidrug resistance (MDR) and ineffective treatment of a wide variety of diseases. In this study, we have designed and constructed a fusion gene (egfp-mexB) of N-terminal mexB with C-terminal egfp, inserted it into a plasmid vector (pMMB67EH), and successfully expressed it in the Δ MexB (MexB deletion) strain of Pseudomonas aeruginosato create a new strain that expresses MexA-(EGFP-MexB)-OprM. We characterized the fusion gene using gel electrophoresis and DNA sequencing, and determined its expression in live …

The Effect Of Various Pathologies On Bone Quality, Daniel S. Porter

Theses and Dissertations--Biomedical Engineering

Bone’s ability to resist fracture is often ignored until a low-energy fracture occurs. Patients with Chronic Kidney Disease (CKD) or osteoporosis are at an increased risk of low-energy fracture. Generally, fracture risk is evaluated by using a bone mineral density (BMD) test. BMD values; however, do not fully predict bone’s ability to resist fracture. This suggests that other parameters may be involved. Bone quality is the term used to describe these parameters, which are categorized into three groups: structural, material, and microdamage. The aim of this dissertation research was to examine whether bone quality was altered in patients who: 1) …

Ph Regulation And The Assessment Of Renal Injury Biomarkers In A Warm Perfusion Renal Allograft Preservation System, Aaron Meyer

Legacy Theses & Dissertations (2009 - 2024)

A shortage of viable kidneys available for transplantation exists today, as the number of individuals waiting for a kidney transplant continues to grow while the number of kidneys available each year for transplantation has remained stagnant. The development of technology that will allow for transplantation of organs that currently may be considered too damaged for transplant will provide access to a large number of donors who have experienced traumatic injury deaths resulting in loss of cardiac function. These potential donors account for the majority of all traumatic injury deaths in intensive care units, however their organs have experienced a degree …

Expanding Applications Of The Nano Intravital Device As A Platform For Exploring Tumor Microenvironments, Michael Padgen

Legacy Theses & Dissertations (2009 - 2024)

The tumor microenvironment has been demonstrated to be a key determinant in the progression of cancer. Unfortunately, the mechanisms behind the different microenvironments (cytokine gradients, hypoxia, hypoglycemia, etc) have not been fully elucidated. Identifying these mechanisms can lead to targeted, individualized therapy to prevent metastasis. The Nano Intravital Device (NANIVID) is a microfabricated, implantable device designed to initiate specific microenvironments in vivo so that the time course of the effects can be observed. With both spatial and temporal control over the induced environments, the affected regions of the tumor can be compared to the rest of the tumor. The NANIVID …

Biomacromolecule Conjugated Nanofiber Scaffold For Salivary Gland Tissue Engineering, Kavitha Jayarathanam

Legacy Theses & Dissertations (2009 - 2024)

ABSTRACT :

Pretreatment Of Scenedesmus Sp. Biomass As A Potential Anaerobic Digestion Substrate, Xinyi E, Czarena Crofcheck

Biosystems and Agricultural Engineering Faculty Publications

Algal biomass can be a potential substrate for anaerobic digestion. However, raw algae cells show a resistance to biological degradation, resulting in a slower methane production rate. Varying thermal and chemical pretreatments of algal biomass were investigated in an attempt to increase soluble organic matter (SOM) yield, which would result in enhanced methane production during subsequent anaerobic digestion. Scenedesmus sp. was harvested using three different procedures: with flocculation, with flocculation and drying, and without flocculation or drying. For all pretreatments and algae types, fluorescence micrographs were obtained to visually confirm the degradation of the algal cell walls. A complete 2 …

Autologous Platelet Rich Plasma (Platelet Gel): An Appropriate Intervention For Salvaging Cardiac Myocytes Under Oxidative Stress After Myocardial Infarction, Barbara Y. Hargrave

Bioelectrics Publications

Background: The prompt restoration of blood flow (reperfusion) to the ischemic myocardium after an acute myocardial infarction is critical to the survival of non damaged heart tissue. However, reperfusion is responsible for additional myocardial damage. Our objective was to investigate the role of autologous platelet rich plasma or platelet gel prepared using nanosecond pulsed electric fields (nsPEFs) in improving left ventricular mechanical function after ischemic reperfusion.

Methods: The hearts of nine adult female New Zealand White rabbits were perfused using the Langendorff model. The hearts were exposed to global ischemia for 30 min and reperfused for 60 min. The hearts …

Information Literacy Skills As A Critical Thinking Framework In The Undergraduate Engineering Curriculum, James E. Van Loon, Heather L. Lai

Library Scholarly Publications

Information Literacy (IL) instruction embedded into the engineering design curriculum can provide a framework for the development of critical thinking skills which are essential for students to master to solve open-ended engineering problems. At Wayne State University, a lecturer in biomedical engineering (BME) and a science librarian are collaborating in an ongoing effort to integrate IL instruction into the BME undergraduate design curriculum. The paper will provide a vision and rationale for integrating IL instruction into the engineering design curriculum, and discuss aspects of the Wayne State effort to effect this integration. A review of the place of critical thinking …

Neuroimage Analysis Of Traumatic Brain Injury In Human Patients, Hardik Doshi

Wayne State University Theses

CEREBRAL HEMODYNAMIC CHANGES IN MILD TRAUMATIC BRAIN INJURY AT THE ACUTE STAGE

by

Hardik J. Doshi

May 2014

Advisor: Dr. Zhifeng Kou

Major: Biomedical Engineering

Degree: Master of Science

There are more than 1.7 million reported traumatic brain injury (TBI) cases every year in the United States alone. Out of all these, more than 85% are mild TBI (mTBI) and it has significant socio-economic impacts. The motivation towards this study is to measure changes in the arterial cerebral blood flow (CBF) and venous oxygen saturation by combining Arterial Spin Labeling (ASL) and Susceptibility Weighted Imaging and Mapping (SWIM) techniques and …

Diffusion Tensor Imaging Of The Spinal Cord: Insights From Animal And Human Studies, Aditya Vedantam, Michael B. Jirjis, Brian D. Schmit, Marjorie C. Wang, John L. Ulmer, Shekar N. Kurpad

Biomedical Engineering Faculty Research and Publications

Diffusion tensor imaging (DTI) provides a measure of the directional diffusion of water molecules in tissues. The measurement of DTI indexes within the spinal cord provides a quantitative assessment of neural damage in various spinal cord pathologies. DTI studies in animal models of spinal cord injury indicate that DTI is a reliable imaging technique with important histological and functional correlates. These studies demonstrate that DTI is a noninvasive marker of microstructural change within the spinal cord. In human studies, spinal cord DTI shows definite changes in subjects with acute and chronic spinal cord injury, as well as cervical spondylotic myelopathy. …

Benefits Of Industry Involvement In Multidisciplinary Capstone Design Courses, Jay R. Goldberg, Vikram Cariapa, George Corliss, Kate Kaiser

Biomedical Engineering Faculty Research and Publications

Opportunities for industry involvement in capstone design courses go beyond industry sponsorship of capstone design projects. Representatives from industry can serve as guest lecturers, curriculum advisors, and design project sponsors and team mentors. Since 2000, industry participation has been a core part of the capstone design course at Marquette University. Practicing engineers provide a relevant, practical real-world perspective of their topic, reinforcing its importance to professional engineering practice. Students and faculty benefit from the up-to-date treatment of the topic provided by guest speakers from industry who have expertise in the topic and are willing to share their experiences with students. …

Age-Related Differentiation Of Sensorimotor Control Strategies During Pursuit And Compensatory Tracking, Megan L. Heenan, Robert A. Scheidt, Scott A. Beardsley

Biomedical Engineering Faculty Research and Publications

Motor control deficits during aging have been well-documented. Various causes of neuromotor decline, including both peripheral and central neurological deficits, have been hypothesized. Here, we use a model of closed-loop sensorimotor control to examine the functional causes of motor control deficits during aging. We recruited 14 subjects aged 19-61 years old to participate in a study in which they performed single-joint compensatory and pursuit tracking tasks with their dominant hand. We found that visual response delay and visual noise increased with age, while reliance on visual feedback, especially during compensatory tracking decreased. Increases in visual noise were also positively correlated …

Submovements During Reaching Movements After Stroke, Lucia S. Simo, Davide Piovesan, Jozsef Laczko, Claude Ghez, Robert A. Scheidt

Biomedical Engineering Faculty Research and Publications

Neurological deficits after cerebrovascular accidents very frequently disrupt the kinematics of voluntary movements with the consequent impact in daily life activities. Robotic methodologies enable the quantitative characterization of specific control deficits needed to understand the basis of functional impairments and to design effective rehabilitation therapies. In a group of right handed chronic stroke survivors (SS) with right side hemiparesis, intact proprioception, and differing levels of motor impairment, we used a robotic manipulandum to study right arm function during discrete point-to-point reaching movements and reciprocal out-and-back movements to visual targets. We compared these movements with those of neurologically intact individuals (NI). …