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Biomedical Engineering and Bioengineering Commons™
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Articles 151 - 155 of 155
Full-Text Articles in Biomedical Engineering and Bioengineering
Polystyrene Microsphere And 5-Fluorouracil Release From Custom Designed Wound Dressing Films, Maryam Mobed-Miremadi, Raki Komarla Nagendra, Sujana Lakshmi Ramachandruni, Jason James Rook, Mallika Keralapura, Michel Goedert
Polystyrene Microsphere And 5-Fluorouracil Release From Custom Designed Wound Dressing Films, Maryam Mobed-Miremadi, Raki Komarla Nagendra, Sujana Lakshmi Ramachandruni, Jason James Rook, Mallika Keralapura, Michel Goedert
Bioengineering
Custom-designed wound dressing films of chitosan and alginate have been prepared by a casting/solvent evaporation method for hydrophobic therapeutic agent encapsulation. In this parametric study, the propylene glycol (PG) and calcium chloride (CaCl2) concentrations were varied for chitosan and alginate films, respectively. Mechanical and chemical inter-related responses under observations included thickness (th), elasticity (E), tensile strength (TS), sorption ability (S%) and kinetics of in-vitro drug release, specifically in terms of membrane time to burst (tB) and duration of release (tR). As shown by results of a one tailed t-test significance testing at the 95% confidence interval (α = …
Soft Microenvironments Promote The Early Neurogenic Differentiation But Not Self-Renewal Of Human Pluripotent Stem Cells, Albert Keung, Prashanth Asuri, Sanjay Kumar, David Schaffer
Soft Microenvironments Promote The Early Neurogenic Differentiation But Not Self-Renewal Of Human Pluripotent Stem Cells, Albert Keung, Prashanth Asuri, Sanjay Kumar, David Schaffer
Bioengineering
Human pluripotent stem cells (hPSCs) are of great interest in biology and medicine due to their ability to self-renew and differentiate into any adult or fetal cell type. Important efforts have identified biochemical factors, signaling pathways, and transcriptional networks that regulate hPSC biology. However, recent work investigating the effect of biophysical cues on mammalian cells and adult stem cells suggests that the mechanical properties of the microenvironment, such as stiffness, may also regulate hPSC behavior. While several studies have explored this mechanoregulation in mouse embryonic stem cells (mESCs), it has been challenging to extrapolate these findings and thereby explore their …
Directed Evolution Of Adeno-Associated Virus For Enhanced Gene Delivery And Gene Targeting In Human Pluripotent Stem Cells, Prashanth Asuri, Melissa Bartel, Tandis Vazin, Jae-Hyung Jang, Tiffany Wong, David Schaffer
Directed Evolution Of Adeno-Associated Virus For Enhanced Gene Delivery And Gene Targeting In Human Pluripotent Stem Cells, Prashanth Asuri, Melissa Bartel, Tandis Vazin, Jae-Hyung Jang, Tiffany Wong, David Schaffer
Bioengineering
Efficient approaches for the precise genetic engineering of human pluripotent stem cells (hPSCs) can enhance both basic and applied stem cell research. Adenoassociated virus (AAV) vectors are of particular interest for their capacity to mediate efficient gene delivery to and gene targeting in various cells. However, natural AAV serotypes offer only modest transduction of human embryonic and induced pluripotent stem cells (hESCs and hiPSCs), which limits their utility for efficiently manipulating the hPSC genome. Directed evolution is a powerful means to generate viral vectors with novel capabilities, and we have applied this approach to create a novel AAV variant with …
An Evolved Adeno-Associated Viral Variant Enhances Gene Delivery And Gene Targeting In Neural Stem Cells, Jae-Hyung Jang, James Koerber, Jung-Suk Kim, Prashanth Asuri, Tandis Vazin, Melissa Bartel, Albert Keung, Inchan Kwan, David Schaffer
An Evolved Adeno-Associated Viral Variant Enhances Gene Delivery And Gene Targeting In Neural Stem Cells, Jae-Hyung Jang, James Koerber, Jung-Suk Kim, Prashanth Asuri, Tandis Vazin, Melissa Bartel, Albert Keung, Inchan Kwan, David Schaffer
Bioengineering
Gene delivery to, and gene targeting in, stem cells would be a highly enabling technology for basic science and biomedical application. Adeno-associated viral (AAV) vectors have demonstrated the capacity for efficient delivery to numerous cells, but their application to stem cells has been limited by low transduction efficiency. Due to their considerable advantages, however, engineering AAV delivery systems to enhance gene delivery to stem cells may have an impact in stem cell biology and therapy. Therefore, using several diverse AAV capsid libraries—including randomly mutagenized, DNA shuffled, and random peptide insertion variants—we applied directed evolution to create a “designer” AAV vector …
Preparation And Characterization Of Protein-Nanotube Conjugates, Jonathan S. Dordick, Dhiral A. Shah, Ravindra C. Pangule, Shyam Sundhar Bale, Prashanth Asuri, Amit Joshi, Akhilesh Banerjee, David Vance, Ravi S. Kane
Preparation And Characterization Of Protein-Nanotube Conjugates, Jonathan S. Dordick, Dhiral A. Shah, Ravindra C. Pangule, Shyam Sundhar Bale, Prashanth Asuri, Amit Joshi, Akhilesh Banerjee, David Vance, Ravi S. Kane
Bioengineering
This chapter describes methods of immobilizing proteins on carbon nanotubes, using two different routes—physical adsorption and covalent attachment. We also provide an overview on how such conjugates can be characterized with the help of various techniques, such as Raman, Fourier transform infrared (FT-IR), circular dichroism (CD), and fluorescence spectroscopies, in addition to the standard enzyme kinetic analyses of activity and stability. Both the attachment routes—covalent and noncovalent—could be used to prepare protein conjugates that retained a significant fraction of their native structure and function; furthermore, the protein conjugates were operationally stable, reusable, and functional even under harsh denaturing conditions. These …