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Articles 6601 - 6630 of 8951
Full-Text Articles in Engineering
Enabling Low Cost Wifi-Based Traffic Monitoring System Using Deep Learning, Sayan Sahu
Enabling Low Cost Wifi-Based Traffic Monitoring System Using Deep Learning, Sayan Sahu
Electronic Theses and Dissertations
A traffic monitoring system (TMS) is an integral part of Intelligent Transportation Systems (ITS) for traffic analysis and planning. However, covering huge miles of rural highways (119,247 miles in U.S.) with a large number of TMSs is a very challenging problem due to the cost issue. This paper aims to address the problem by developing a low-cost and portable TMS called DeepWiTraffic based on COTs WiFi devices. The proposed system enables accurate vehicle detection (counting) and classification by exploiting the unique WiFi Channel State Information (CSI) of passing vehicles. Spatial and temporal correlations of CSI amplitude and phase data are …
Evaluation Of The Moisture-Induced Damage Potential Of Asphalt Mixes And Asphalt Binder-Aggregate Systems, Rajan Acharya
Evaluation Of The Moisture-Induced Damage Potential Of Asphalt Mixes And Asphalt Binder-Aggregate Systems, Rajan Acharya
Electronic Theses and Dissertations
The current thesis presents the findings of a study conducted on asphalt mixes to evaluate their moisture-induced damage potential through testing of the plant produced asphalt mixes in South Dakota using local aggregates and commonly used asphalt binders. The moisture-induced damage potentials of the asphalt mixes were evaluated by conducting tensile strength ratio (TSR) and modified Semicircular Bend (SCB) tests. The effect of moisture on asphalt binder-aggregate adhesion was evaluated by conducting binder bond strength (BBS) test on binder-aggregate systems. The asphalt mixes tested in this study included a hot mix asphalt (HMA) containing hydrated lime (1% by the weight …
Application Of Drainage Water Management And Saturated Buffers For Conservation Drainage In South Dakota, Abhinav Sharma
Application Of Drainage Water Management And Saturated Buffers For Conservation Drainage In South Dakota, Abhinav Sharma
Electronic Theses and Dissertations
Edge of field practices such as drainage water management (DWM) and saturated buffers can reduce nutrient transport from croplands to surface waterbodies. DWM uses stackable weir boards in a control structure to manipulate the water table depth throughout the cropping season and reduce the amount of nutrient rich water draining out from the field. Saturated buffers, on the other hand, use a control structure to divert water draining out from the cropland to a vegetative strip via a subsurface tile installed parallel to a waterway. For the saturated buffer systems, a combination of natural denitrification, nitrogen mineralization, and plant uptake …
Cross Calibration And Validation Of Landsat 8 Oli And Sentinel 2a Msi, M. M. Farhad
Cross Calibration And Validation Of Landsat 8 Oli And Sentinel 2a Msi, M. M. Farhad
Electronic Theses and Dissertations
This work describes a proposed radiometric cross calibration between the Landsat 8 Operational Land Imager (OLI) and Sentinel 2A Multispectral Instrument (MSI) sensors. The cross calibration procedure involves i) correction of the MSI data to account for spectral band differences with the OLI; and ii) correction of BRDF effects in the data from both sensors using a new model accounting for the view zenith/azimuth angles in addition to the solar zenith/view angles. Following application of the spectral and BRDF corrections, standard least-squares linear regression is used to determine the cross calibration gain and offset in each band. Uncertainties related to …
A Quantitative Environmental Assessment Of Incorporating Torrefaction Into Farming Enterprises In Eastern South Dakota, Dinesh Fuyal
A Quantitative Environmental Assessment Of Incorporating Torrefaction Into Farming Enterprises In Eastern South Dakota, Dinesh Fuyal
Electronic Theses and Dissertations
The use of renewable energy sources has been increasing in the recent years due to population growth and environmental concerns. Biomass is a promising energy source that can be used to produce biofuels or torrefied pellets. Torrefied biomass may be used in power plants, industrial and residential heating, feedstocks for gasification, air and water filtrating, and soil amendment. The interest of torrefied pellets as energy sources for various applications has been increased in the recent years due to the concerns about energy security and environmental issues. This study focuses on the economic and environmental assessment of agricultural feedstocks like corn …
Comparison Of Historical Precipitation For Aberdeen, Ipswich, And Eureka, South Dakota, Uday Singh Kshatriya
Comparison Of Historical Precipitation For Aberdeen, Ipswich, And Eureka, South Dakota, Uday Singh Kshatriya
Electronic Theses and Dissertations
The availability of water resources varies from time to time with the climate conditions. Keeping track of climate conditions would eventually benefit for the future predictions. The objective of this research was to compare and contrast the climate especially for Aberdeen, Ipswich and Eureka, South Dakota utilizing the historical monthly precipitation records and to develop the techniques that are reliable to estimate the missing data. For these necessary statistical analyses were performed using the monthly precipitation data from High Plains Regional Climate Center (HPRCC) for the annual and 8-Year periods for Aberdeen, Ipswich and Eureka, South Dakota. 63 water years …
An Integrated Method For Forecasting Well Deliverability In Gas Condensate Reservoirs With Bottom Aquifer Drive, Abdulaziz Ellafi, Ralph E. Flori, Shari Dunn-Norman
An Integrated Method For Forecasting Well Deliverability In Gas Condensate Reservoirs With Bottom Aquifer Drive, Abdulaziz Ellafi, Ralph E. Flori, Shari Dunn-Norman
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
Gas condensate reservoirs constitute a significant portion of global hydrocarbon reserves. In these reservoirs, liquids develop in the pore space once bottomhole pressure falls below dew point. This results in the formation of a liquid bank near the wellbore region which decreases gas mobility, which then reduces gas inflow. In such complex reservoirs, it is important to correctly describe PVT impacts, adjustments to well test analysis and inflow performance, and then combine all effects in the reservoir analysis. The literature contains many references to individual adjustments of PVT analysis, well testing, or inflow performance for gas condensate reservoirs, but few …
Miscible Gases Based Eor In Unconventional Liquids Rich Reservoirs: What We Can Learn, Dheiaa Alfarge, Mortadha Alsaba, Mingzhen Wei, Baojun Bai
Miscible Gases Based Eor In Unconventional Liquids Rich Reservoirs: What We Can Learn, Dheiaa Alfarge, Mortadha Alsaba, Mingzhen Wei, Baojun Bai
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
Over the last decade, Unconventional Liquids Rich Reservoirs (ULR) have become the main target for oil and gas investors as conventional formations started to deplete and diminish in numbers. These unconventional plays have a huge oil reserve; however, the primary oil recovery factor is predicted to be less than 10%. Unconventional Improved Oil Recovery (UIOR) techniques are still a new concept in the oil industry since there is no commercial project reported for any IOR technique yet. Miscible gas based EOR technique might be the most potential strategy to improve oil recovery in such complex plays. In this study, a …
New Insights Of Low Salinity Water Flooding In Low Permeability-Low Porosity Clay Rich Sandstone Reservoir, Ali K. Alhuraishawy, Baojun Bai, Mingzhen Wei, Waleed H. Al-Bazzaz
New Insights Of Low Salinity Water Flooding In Low Permeability-Low Porosity Clay Rich Sandstone Reservoir, Ali K. Alhuraishawy, Baojun Bai, Mingzhen Wei, Waleed H. Al-Bazzaz
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
One of the EOR technologies is low-salinity water flooding (LSWF) is an EOR method that operates at a lower cost than other EOR methods, which makes it a preferred area of interest for oil industry economists, who continue to call for EOR costs to come down. The aim is to minimize the possible cost to recover as much oil as possible. The performance of low salinity waterflooding in decreasing residual oil saturation in low permeability-porosity clay-rich sandstone reservoir. Porosity test, core flooding test, XRD and scanning electron microscopy (SEM) tests were achieved in this study. Salinity effect on the porosity, …
Application Of Integrated Reservoir Geomechanics For Well Planning Of High-Angled Wells In A Mature Oil Field, Ahmed K. Abbas, Ralph E. Flori, Yousif M.B. Al-Asadi, Mortadha Alsaba, Nasser A. Al-Hamad
Application Of Integrated Reservoir Geomechanics For Well Planning Of High-Angled Wells In A Mature Oil Field, Ahmed K. Abbas, Ralph E. Flori, Yousif M.B. Al-Asadi, Mortadha Alsaba, Nasser A. Al-Hamad
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
Zubair oil reservoir has a significant potential to contribute to the petroleum supply in Iraq. Drilling highly deviated wells is a commonly used approach to enhance the production in mature fields. Drilling these wells in the Zubair Formation has been a challenge due to severe wellbore instability issues. This study presents how integrated reservoir geomechanics was used for efficient well planning and safe drilling of nearly horizontal wells (75° Inclination) through Zubair depleted sandstone oil reservoir. In this study, an integrated workflow was implemented mainly to build a geomechanical model using offset well data. The in-situ principal stresses and their …
Learning From Experience: An Automatic Ph Neutralization System Using Hybrid Fuzzy System And Neural Network, Ethar H.K. Alkamil, Seaar Al-Dabooni, Ahmed K. Abbas, Ralph Flori, Donald C. Wunsch
Learning From Experience: An Automatic Ph Neutralization System Using Hybrid Fuzzy System And Neural Network, Ethar H.K. Alkamil, Seaar Al-Dabooni, Ahmed K. Abbas, Ralph Flori, Donald C. Wunsch
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
In oil and gas industry, the pH level is one of the most important indicators of mud contamination while drilling. Although the process has simple components, the pH neutralization process is complicated in the mud circulation system. This difficulty is due to the high nonlinearity of the process. In this paper, the fuzzy neural network (FNN) is integrated to the fuzzy logic controller (FLC) to create a system which identifies pH fluctuation into a drilling mud system, assesses and signals its severity. And then, it automatically actuates a chemical treating fluids valve (CTFV), such as Caustic Soda (CS), for neutralizing …
Using Managed Pressure Drilling To Reduce Stuck Pipe Problem, Ethar H.K. Alkamil, Husam R. Abbood, Ralph E. Flori, George Medley, Sagar Nauduri, Mortadha Alsaba
Using Managed Pressure Drilling To Reduce Stuck Pipe Problem, Ethar H.K. Alkamil, Husam R. Abbood, Ralph E. Flori, George Medley, Sagar Nauduri, Mortadha Alsaba
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
Differences between mud pressure in a wellbore and pore pressure in high permeability rocks can lead to differential sticking, particularly when drilling deviated wells and encountering mud losses. Several solutions, all challenging, can be used to address this problem, where the conventional mitigation method of managing the mud weight (MW) is often used. However, managed pressure drilling (MPD) offers a promising solution with a positive risk-adjusted cost and other benefits. Wells in the E oilfield in southern Iraq are typically drilled overbalanced and therefore often experience a high percentage of non-productive time (NPT) due to differential sticking. This study evaluates …
A Novel Ultra-Low Interfacial Tension Nanofluid For Enhanced Oil Recovery In Super-Low Permeability Reservoirs, Derong Xu, Baojun Bai, Ziyu Meng, Qiong Zhou, Zhe Li, Yao Lu, Hairong Wu, Jirui Hou, Wanli Kang
A Novel Ultra-Low Interfacial Tension Nanofluid For Enhanced Oil Recovery In Super-Low Permeability Reservoirs, Derong Xu, Baojun Bai, Ziyu Meng, Qiong Zhou, Zhe Li, Yao Lu, Hairong Wu, Jirui Hou, Wanli Kang
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
The exploration and development of super-low permeability reservoirs have become a global focus in recent years. However, conventional flooding systems commonly face problems of high injection pressure and poor displacement efficiency in super-low permeability reservoirs. Thus, it is imperative to find new flooding agents that tackle such problems. In this study, a novel ultra-low interfacial tension (IFT) nanofluid was formulated, consisting of surfactants to achieve ultra-low IFT and silica nanoparticles to reduce injection pressure. The compatibility test between the surfactants and silica nanoparticles in 10,000 mg/L NaCl solution at 90 °C was conducted to ensure their adaption to harsh reservoir …
Cyber-Physical Embedded Systems With Transient Supervisory Command And Control: A Framework For Validating Safety Response In Automated Collision Avoidance Systems, Daniel K. Trembley
Cyber-Physical Embedded Systems With Transient Supervisory Command And Control: A Framework For Validating Safety Response In Automated Collision Avoidance Systems, Daniel K. Trembley
Graduate Dissertations and Theses
The ability to design and engineer complex and dynamical Cyber-Physical Systems (CPS) requires a systematic view that requires a definition of level of automation intent for the system. Since CPS covers a diverse range of systemized implementations of smart and intelligent technologies networked within a system of systems (SoS), the terms “smart” and “intelligent” is frequently used in describing systems that perform complex operations with a reduced need of a human-agent. The difference between this research and most papers in publication on CPS is that most other research focuses on the performance of the CPS rather than on the correctness …
Performance Analysis Of A Computer-Aided Detection System For Lung Nodules In Ct At Different Slice Thicknesses, Barath Narayanan, Russell C. Hardie, Temesguen Messay Kebede
Performance Analysis Of A Computer-Aided Detection System For Lung Nodules In Ct At Different Slice Thicknesses, Barath Narayanan, Russell C. Hardie, Temesguen Messay Kebede
Electrical and Computer Engineering Faculty Publications
We study the performance of a computer-aided detection (CAD) system for lung nodules in computed tomography (CT) as a function of slice thickness. In addition, we propose and compare three different training methodologies for utilizing nonhomogeneous thickness training data (i.e., composed of cases with different slice thicknesses). These methods are (1) aggregate training using the entire suite of data at their native thickness, (2) homogeneous subset training that uses only the subset of training data that matches each testing case, and (3) resampling all training and testing cases to a common thickness. We believe this study has important implications for …
Datanet: Deep Learning Based Encrypted Network Traffic Classification In Sdn Home Gateway, Pan Wang, Feng Ye, Xuejiao Chen, And Yi Qian
Datanet: Deep Learning Based Encrypted Network Traffic Classification In Sdn Home Gateway, Pan Wang, Feng Ye, Xuejiao Chen, And Yi Qian
Electrical and Computer Engineering Faculty Publications
A smart home network will support various smart devices and applications, e.g., home automation devices, E-health devices, regular computing devices, and so on. Most devices in a smart home access the Internet through a home gateway (HGW). In this paper, we propose a software-defined- network (SDN)-HGW framework to better manage distributed smart home networks and support the SDN controller of the core network. The SDN controller enables efficient network quality-of-service management based on real-time traffic monitoring and resource allocation of the core network. However, it cannot provide network management in distributed smart homes. Our proposed SDN-HGW extends the control to …
Can Public Transportation Compete With Automated And Connected Cars?, Ralph Buehler
Can Public Transportation Compete With Automated And Connected Cars?, Ralph Buehler
Journal of Public Transportation
Over the next 30 years, technological innovation will make automobile travel more convenient. Automated and connected vehicles will perform an increasing number of driving tasks without human input and will lure customers away from traditional public transportation. This paper first explores key characteristics of public transportation demand in the United States today--based on an international comparison with other Western countries. Next, the paper provides potential pathways on how public transportation agencies and local governments in the United States could respond to the emergence of automated and connected vehicles. The paper argues that space efficiency in urbanized areas and the rush …
Future Of Rural Transit, Jill Hough, Ali Rahim Taleqani
Future Of Rural Transit, Jill Hough, Ali Rahim Taleqani
Journal of Public Transportation
This paper provides a contemplative description of the future of rural public transportation. It considers emerging technologies along with their long-term implications and corresponding impacts on rural communities. The authors used their collective knowledge to identify key drivers of change in rural areas. As a result, the authors expect the future definition of rural areas to change and a new geographical classification to emerge. This classification is a continuum of population density gradient from highly populated urban areas to sparsely populated areas. The paper also suggests that automated vehicles and hologram telecommuting could dominate the U.S. transportation industry, even in …
Just Around The Corner: The Future Of U.S. Public Transportation, Steven E. Polzin
Just Around The Corner: The Future Of U.S. Public Transportation, Steven E. Polzin
Journal of Public Transportation
No abstract provided.
Is It Time For A Public Transit Renaissance?: Navigating Travel Behavior, Technology, And Business Model Shifts In A Brave New World, Susan Shaheen, Adam Cohen
Is It Time For A Public Transit Renaissance?: Navigating Travel Behavior, Technology, And Business Model Shifts In A Brave New World, Susan Shaheen, Adam Cohen
Journal of Public Transportation
Travel behavior is undergoing a period of significant change in the United States. In 2016, public transit ridership fell in almost all major U.S. metropolitan regions. While Americans are still heavily dependent on the personal automobile for mobility, technological and societal changes are transforming how mobility is accomplished. This paper reviews the convergence of five trends leading to fundamental changes in public transportation: (1) changing generational behavior toward suburbanization and automobility; (2) new attitudes toward information communications technology; (3) shifting attitudes toward sharing and mobility on demand; (4) innovative alternatives to work and non-work travel; and (5) an increasing number …
Transit In The 2000s: Where Does It Stand And Where Is It Headed?, Michael Manville, Brian D. Taylor, Evelyn Blumenberg
Transit In The 2000s: Where Does It Stand And Where Is It Headed?, Michael Manville, Brian D. Taylor, Evelyn Blumenberg
Journal of Public Transportation
U.S. public transit has experienced something of a renaissance in the 2000s, with per capita service levels increasing nationwide and public investment growing even faster—particularly expenditures on rail transit. Despite this expansion, overall transit patronage has been relatively flat, and has declined significantly since 2014. What is behind these trends, and what do they portend for the future of transit? In this paper we consider three challenges shaping transit today and in the years ahead: (1) the asymmetry of transit supply and use make it especially vulnerable to changes and disruptions; (2) many of the factors that determine transit ridership, …
To Predict With Confidence, Plan For Freedom, Jarrett Walker
To Predict With Confidence, Plan For Freedom, Jarrett Walker
Journal of Public Transportation
No abstract provided.
Study Of Gas Production From Shale Reservoirs With Multi-Stage Hydraulic Fracturing Horizontal Well Considering Multiple Transport Mechanisms, Chaohua Guo, Mingzhen Wei, Hong Liu
Study Of Gas Production From Shale Reservoirs With Multi-Stage Hydraulic Fracturing Horizontal Well Considering Multiple Transport Mechanisms, Chaohua Guo, Mingzhen Wei, Hong Liu
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
Development of unconventional shale gas reservoirs (SGRs) has been boosted by the advancements in two key technologies: horizontal drilling and multi-stage hydraulic fracturing. A large number of multi-stage fractured horizontal wells (MsFHW) have been drilled to enhance reservoir production performance. Gas flow in SGRs is a multi-mechanism process, including: desorption, diffusion, and non-Darcy flow. The productivity of the SGRs with MsFHW is influenced by both reservoir conditions and hydraulic fracture properties. However, rare simulation work has been conducted for multi-stage hydraulic fractured SGRs. Most of them use well testing methods, which have too many unrealistic simplifications and assumptions. Also, no …
Utility-Scale Solar Photovoltaic Hosting Potential Of Historic Mill Sites In Humboldt County, California, Michael Avcollie
Utility-Scale Solar Photovoltaic Hosting Potential Of Historic Mill Sites In Humboldt County, California, Michael Avcollie
Cal Poly Humboldt theses and projects
The adoption of Redwood Coast Energy Authority’s (RCEA’s) Community Choice Energy program in May of 2017 across Humboldt County has sparked interest in locally produced energy from solar photovoltaics (PV). RCEA has a goal of bringing 5 Megawatts (MW) of local, utility-scale, solar PV under contract by 2018 and 15 MW by 2023. Humboldt County’s former mill sites offer features that make them suitable for hosting PV development. This study performed an inventory and analysis of these sites to answer two questions: Can Humboldt County’s former mill sites provide the hosting capacity for15 MW of local, utility-scale solar PV? Which …
Single Camera Photogrammetry Matlab Solver Developed For Automation Of The Oil Interferometry Process, Hunter Michael Dunn
Single Camera Photogrammetry Matlab Solver Developed For Automation Of The Oil Interferometry Process, Hunter Michael Dunn
Master's Theses
Over the last 20 years, Gregory G. Zilliac of the NASA AMES Research Center has been in continuous development of a fringe-imaging skin friction PC application used in oil interferometry analysis. This application, CXWIN5G, allows users to analyze propagation of oil smears across an aerodynamic surface using photogrammetry. The purpose of this thesis is to investigate the feasibility of increasing the level of automation currently found in CXWIN5G by developing a MATLAB solver capable of determining oil smear geometry with minimal user input.
There are two main automation goals of this thesis that are reflected in the core of the …
Hydrophobic Surface Modification Of Polymethyl Methacrylate By Two-Dimensional Plasma Jet Array At Atmospheric Pressure, Feng Liu, Meiling Cai, Bo Zhang, Zhi Fang, Chunqi Jiang, Kostya Ken Ostrikov
Hydrophobic Surface Modification Of Polymethyl Methacrylate By Two-Dimensional Plasma Jet Array At Atmospheric Pressure, Feng Liu, Meiling Cai, Bo Zhang, Zhi Fang, Chunqi Jiang, Kostya Ken Ostrikov
Bioelectrics Publications
Recently developed atmospheric pressure plasma jet (APPJ) is considered as a novel and efficient technique for uneven surfaces processing and APPJ array effectively expands the treatment area of a single APPJ. In this paper, a two-dimensional (2D) APPJ array in Ar/tetramethylsilane (TMS) is used to improve the surface hydrophobicity of polymethyl methacrylate (PMMA) by depositing polymerized silicalike clusters on the jet-PMMA interface. The electrical and optical characteristics of the 2D Ar/TMS APPJ array are measured to optimize experimental conditions. The wettability of jet-PMMA interface is assessed by measuring water contact angle, which increases from 65° to a maximum value of …
Wild-Type P53 Enhances Endothelial Barrier Function By Mediating Rac1 Signalling And Rhoa Inhibition, Nektarios Barabutis, Christiana Dimitropoulou, Betsy Gregory, John D. Catravas
Wild-Type P53 Enhances Endothelial Barrier Function By Mediating Rac1 Signalling And Rhoa Inhibition, Nektarios Barabutis, Christiana Dimitropoulou, Betsy Gregory, John D. Catravas
Bioelectrics Publications
Inflammation is the major cause of endothelial barrier hyper-permeability, associated with acute lung injury and acute respiratory distress syndrome. This study reports that p53 "orchestrates" the defence of vascular endothelium against LPS, by mediating the opposing actions of Rac1 and RhoA in pulmonary tissues. Human lung microvascular endothelial cells treated with HSP90 inhibitors activated both Rac1- and P21-activated kinase, which is an essential element of vascular barrier function. 17AAG increased the phosphorylation of both LIMK and cofilin, in contrast to LPS which counteracted those effects. Mouse lung microvascular endothelial cells exposed to LPS exhibited decreased expression of phospho-cofilin. 17AAG treatment …
Alteration Of Metabolite Profiling By Cold Atmospheric Plasma Treatment In Human Myeloma Cells, Dehui Xu, Yujing Xu, Ning Ning, Qingjie Cui, Zhijie Liu, Xiaohua Wang, Dingxin Liu, Hailan Chen, Michael G. Kong
Alteration Of Metabolite Profiling By Cold Atmospheric Plasma Treatment In Human Myeloma Cells, Dehui Xu, Yujing Xu, Ning Ning, Qingjie Cui, Zhijie Liu, Xiaohua Wang, Dingxin Liu, Hailan Chen, Michael G. Kong
Bioelectrics Publications
BACKGROUND: Despite new progress of chemotherapy in multiple myeloma (MM) clinical treatment, MM is still a refractory disease and new technology is needed to improve the outcomes and prolong the survival. Cold atmospheric plasma is a rapidly developed technology in recent years, which has been widely applied in biomedicine. Although plasma could efficiently inactivate various tumor cells, the effects of plasma on tumor cell metabolism have not been studied yet.
METHODS: In this study, we investigated the metabolite profiling of He plasma treatment on myeloma tumor cells by gas-chromatography time-of-flight (GC-TOF) mass-spectrometry. Meanwhile, by bioinformatic analysis such as GO and …
Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma And Induces Innate And Adaptive Memory Immune Mechanisms That Prevent Recurrence, Brittany P. Lassiter, Siqi Guo, Stephen J. Beebe
Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma And Induces Innate And Adaptive Memory Immune Mechanisms That Prevent Recurrence, Brittany P. Lassiter, Siqi Guo, Stephen J. Beebe
Bioelectrics Publications
Nano-pulse stimulation (NPS), previously called nsPEFs, induced a vaccine-like effect after ablation of orthotopic N1-S1 hepatocellular carcinoma (HCC), protecting rats from subsequent challenges with N1-S1 cells. To determine immunity, immune cell phenotypes were analyzed in naïve, treated and protected rats. NPS provides a positive, post-ablation immuno-therapeutic outcome by alleviating immunosuppressive T regulatory cells (Treg) in the tumor microenvironment (TME), allowing dendritic cell influx and inducing dynamic changes in natural killer cells (NKs), NKT-cells and T-lymphocytes in blood, spleen and liver. NPS induced specific increases in NKs and NKT-cells expressing CD8 and activation receptors CD314-NKG2D and CD161 (NK1.1) in the TME …
Tumor Cell Death After Electrotransfer Of Plasmid Dna Is Associated With Cytosolic Dna Sensor Upregulation, Katarina Znidar, Masa Bosnjak, Nina Semenova, Olga N. Pakhomova, Loree Heller, Maja Cemazar
Tumor Cell Death After Electrotransfer Of Plasmid Dna Is Associated With Cytosolic Dna Sensor Upregulation, Katarina Znidar, Masa Bosnjak, Nina Semenova, Olga N. Pakhomova, Loree Heller, Maja Cemazar
Bioelectrics Publications
Cytosolic DNA sensors are a subgroup of pattern recognition receptors (PRRs) and are activated by the abnormal presence of the DNA in the cytosol. Their activation leads to the upregulation of pro-inflammatory cytokines and chemokines and can also induce cell death. The presence of cytosolic DNA sensors and inflammatory cytokines in TS/A murine mammary adenocarcinoma and WEHI 164 fibrosarcoma cells was demonstrated using real time reverse transcription polymerase chain reaction (RT-PCR), western blotting and enzyme-linked immunosorbent assay (ELISA). After electrotransfer of plasmid DNA (pDNA) using two pulse protocols, the upregulation of DNA-depended activator of interferon regulatory factor or Z-DNA binding …