Engineering Commons^™

Field Evaluation Of Arbuscular Mycorrhizal Fungal Colonization In Bacillus Thuringiensis Toxin-Expressing (Bt) And Non-Bt Maize, Tanya E. Cheeke, Mitchell B. Cruzan, Todd N. Rosenstiel

Biology Faculty Publications and Presentations

The cultivation of genetically engineered Bacillus thuringiensis toxin-expressing (Bt) maize continues to increase worldwide, yet the effects of Bt crops on arbuscular mycorrhizal fungi (AMF) in soil are poorly understood. In this field experiment, we investigated the impact of seven different genotypes of Bt maize and five corresponding non-Bt parental cultivars on AMF and evaluated plant growth responses at three different physiological time points. Plants were harvested 60 days (active growth), 90 days (tasseling and starting to produce ears), and 130 days (maturity) after sowing, and data on plant growth responses and percent AMF colonization of roots at each harvest …

A Morphological Analysis Of A Hybrid Swarm Of Native Ulmus Rubra And Introduced U. Pumila (Ulmaceae) In Southeastern Nebraska, Christian Elowsky, Ingrid E. Jordon-Thaden, Robert B. Kaul

Nebraska Center for Biotechnology: Faculty and Staff Publications

The parental species and hybrid swarm of native Ulmus rubra Muhl. and the introduced, naturalized and weedy U. pumila L. were investigated in a 65-km transect in Lancaster, Saunders, and Butler counties in Nebraska. Thirty-two trees of U. rubra, 32 of U. pumila, and 50 of the hybrid swarm were sampled for leaves and buds and subsampled for flowers and fruits. Leaves were measured for petiole length, blade length, width, primary and secondary teeth per cm, number of secondary teeth per primary tooth, and texture. Buds were scored for color and distribution of trichomes. Flowers were sampled for stamen counts …

New Types Of Morpho-Physiological Changes In Cells Exposed To Nanosecond Pulsed Electric Field, Mikhail A. Rassokhin

Theses and Dissertations in Biomedical Sciences

Exposure of cells to a pulsed electric field (PEF) is the basis of multiple techniques and treatments. Nanosecond pulsed electric field (nsPEF) poses unique characteristics to induce subtle cellular effects while preserving cell integrity. Improving understanding of the mechanisms triggered by nsPEF in cells inspires new applications for the nanosecond pulse technology. Although many effects of nsPEF remain unknown, they can be inferred from morpho-physiologic changes, or cell reshaping, that accompany nsPEF exposure. During the exposure cells undergo reshaping that is manifested in swelling and diffuse blebbing. Recently we identified two new distinct forms of reshaping, pseudopod-like blebbing and microvesiculation, …

Preliminary Development Of A Subject Specific Finite Element Model Of The Hindfoot, Liang Dong

Mechanical & Aerospace Engineering Theses & Dissertations

The human hindfoot is a complex structure and its medical treatment can vary from patient to patient. If physicians have access to a patient specific computer model, the optimal treatment option can be explored on the model before preforming it on the patient. The purpose of this study was to create a quasi-static finite element model and to show the stress contour in the cartilage between the bones. Ankle joint contact pressure was collected on one cadaver limb with a 40 lbs ( 178 N) axial load applied and tendon loads applied to simulate the ratio of tendon loading during …

Influence Of Media Composition On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus Utilized For Co2 Mitigation, Czarena L. Crofcheck, Xinyi E, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to increasing combustion of fossil fuels. One possible CO₂ mitigation strategy is the use of microalgae for mitigation of CO₂. This paper focuses on the influence of media composition on the growth rate of two microalgae strains, Chlorella vulgaris and Scenedesmus actus. A KNO₃ based medium was found to work better for Chlorella, while a urea based equivalent worked better for Scenedesmus. The urea based media investigated here resulted in growth similar to that found with previously reported KNO₃ based media. …

Design, Construction And Cost Analysis Of Manufacturing Horse Bits, Erica L. Timmermans

BioResource and Agricultural Engineering

This report discusses the design, fabrication and cost analysis of two unique horse bits. On the market today, there are many different kinds of bits to serve a multitude of purposes. However, this project explored the option of having two different shanks types made of stainless steel in addition to three mouthpieces that are able to fit in both types of shanks.

Design And Development Of A Stair Ascension Assistive Device For Transfemoral Amputees, Casey Michael Barbarino

Master's Theses

Transfemoral amputees around the world experience increased difficulty in climbing stairs due to lack of muscle, balance, and other factors. The loss of a lower limb greatly diminishes the amount of natural force generation provided that is necessary to propel oneself up stairs. This study investigated possible solutions to the problem of stair ascension for transfemoral amputees by the means of designing and developing an externally attachable device to a prosthesis. The number of amputations from military service has greatly increased since 2008, which shows there is a clear need for assistive devices (Wenke, Krueger, & Ficke, 2012). With the …

Settling Performance In Wastewater Fed High Rate Algae Ponds, Elliott Blake Ripley

Master's Theses

Although high rate algae ponds (HRAPs) are a proven wastewater treatment technology with numerous environmental, social, and economic benefits, their widespread use has been hindered by inconsistent and unreliable settling performance. Hence, the goal of this thesis is to investigate how specific operational parameters affect the settling performance of HRAPs. Nine HRAPs (30 m² surface area, 0.3 m depth) were operated as three triplicate sets, with each set run on either a 2, 3, or 4 day HRT continuously from January 25, 2012 through April 11, 2013. Settling performance was determined (i) by measuring the TSS of Imhoff cone …

The Santa Clara, 2013-05-23, Santa Clara University

The Santa Clara

No abstract provided.

The Santa Clara, 2013-05-16, Santa Clara University

The Santa Clara

No abstract provided.

The Santa Clara, 2013-05-09, Santa Clara University

The Santa Clara

No abstract provided.

The Santa Clara, 2013-05-02, Santa Clara University

The Santa Clara

No abstract provided.

Controlling Bacterial Persister Cells With Low Level Electric Currents, Henry Lars Peterson

Renée Crown University Honors Thesis Projects - All

Bacterial persister cells present a growing concern as they inherit the ability to tolerate high concentrations of antibiotics and repopulate after an antibiotic treatment leading to chronic diseases. Pseudomonas aeruginosa causes many human infections including skin infections and those associated with burn injuries, and implanted medical devices, and are associated with Cystic Fibrosis. Recently, the Ren Lab developed a novel approach to eliminate persister cells of P. aeruginosa, including those in biofilms, using low level electric currents. To evaluate the safety of this method and to better understand how the underlying elements, this study focused on the cytotoxicity of …

Biomass Gasification: An Alternative Solution To Animal Waste Management, Hanjing Wu

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The overall goal of this research was to evaluate gasification of animal waste as an alternative manure management strategy, from the standpoints of syngas production and biochar application.

To meet the overall objective, the thermogravimetric characteristics of dairy manure, as a thermochemical conversion feedstock, were studied firstly. Then, gasification technology was applied to dairy manure and feedlot manure using a fluidized-bed laboratory-scale gasifier. In addition, biochar derived from the feedlot manure was examined for its effects on nutrient leaching as a soil amendment. Finally, a life cycle assessment was conducted to evaluate greenhouse gas emissions of two feedlot manure management …

Predicting Groundwater Trading Participation In The Upper Republican River Natural Resource District, Elizabeth M. Juchems

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The goal of this thesis is to predict participation in groundwater trading and the directions of trades among participants. Specifically, the paper considers both formal and informal trading of groundwater used for crop irrigation purposes and attempts to identify those characteristics that predict the probability of trade participation and whether an individual is a buyer or seller of groundwater rights. While the public benefits from efficient use of groundwater include adequate stream flow in hydrologically connected areas and future use of groundwater supplies, there are significant private benefits to landowners especially in water-short areas. Groundwater trading can help move water …

The Geometry And Sensitivity Of Ion-Beam Sculpted Nanopores For Single Molecule Dna Analysis, Ryan Connor Rollings

Graduate Theses and Dissertations

In this dissertation, the relationship between the geometry of ion-beam sculpted solid-state nanopores and their ability to analyze single DNA molecules using resistive pulse sensing is investigated. To accomplish this, the three dimensional shape of the nanopore is determined using energy filtered and tomographic transmission electron microscopy. It is shown that this information enables the prediction of the ionic current passing through a voltage biased nanopore and improves the prediction of the magnitude of current drop signals when the nanopore interacts with single DNA molecules. The dimensional stability of nanopores in solution is monitored using this information and is improved …

Delayed Thrombus Resolution And Fibroproliferative Vascular Wound Healing From Deficiency Of Type Iii Collagen: A Paradoxical Mechanism For Tissue Fragility, Amy J. Reid

Dissertations and Theses (Open Access)

Vascular Ehlers-Danlos syndrome is a heritable disease of connective tissue caused by mutations in COL3A1, conferring a tissue deficiency of type III collagen. Cutaneous wounds heal poorly in these patients, and they are susceptible to spontaneous and catastrophic rupture of expansible hollow organs like the gut, uterus, and medium-sized to large arteries, which leads to premature death. Although the predisposition for organ rupture is often attributed to inherent tissue fragility, investigation of arteries from a haploinsufficient Col3a1 mouse model (Col3a1^+/-) demonstrates that mutant arteries withstand even supraphysiologic pressures comparably to wild-type vessels. We hypothesize that injury …

Bio-Separation Process Improvement Via Genomic Manipulation: Development Of Novel Strains For Use In Immobilized Metal Affinity Chromatography (Imac), Ryan Curtis Haley

Graduate Theses and Dissertations

The dissertation is comprised of three parts. Part I describes proteomic analysis of native bacterial proteins from Escherichia coli (E.coli) that bind during Immobilized Metal Affinity Chromatography (IMAC). Part II describes the value in exploiting proteome based data as a tool toward the design an E. coli expression strain that is particularly useful when Immobilized Metal Affinity Chromatography is employed as the initial capture step of a homologous protein purification process. Part III describes a methodology of chromosomal mapping of all contaminant gene products.

The objective of Part I was to identify all E. coli proteins that bind to Co(II), …

Expression, Production, And Purification Of Novel Therapeutic Proteins, Mckinzie Shea Fruchtl

Graduate Theses and Dissertations

Interest in the production of recombinant proteins consisting of collagen binding domain (CBD) fused to a bioactive material has increased due to the targeting/attachment capabilities of CBD. For example, CBD fusions can be applied to the reversing of bone density loss and the repair of the eardrum, specifically, by choosing an appropriate fusion partner (parathyroid hormone or epidermal growth factor). The production of CBD fusions was examined using batch and fed-batch culturing of Escherichia coli to express the fusion proteins, and affinity chromatography to isolate the final product.

Different medium formulations, feeding strategies, and induction methods were tested in order …

The Santa Clara, 2013-04-25, Santa Clara University

The Santa Clara

No abstract provided.

2013 - The Eighteenth Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Eighteenth Annual Symposium of Student Scholars, held on April 25, 2013. Includes abstracts from the presentations and posters.

The Santa Clara, 2013-04-18, Santa Clara University

The Santa Clara

No abstract provided.

12th Annual Undergraduate Student Symposium, Farquhar Honors College

Undergraduate Student Symposium

The Undergraduate Student Symposium, sponsored by the Farquhar College of Arts and Sciences, presents student projects through presentations, papers, and poster displays. The event serves as a “showcase” demonstrating the outstanding scholarship of undergraduate students at NSU. The Symposium is open to undergraduate students from all disciplines. Projects cover areas of student scholarship ranging from the experimental and the applied to the computational, theoretical, artistic, and literary. They are taken from class assignments as well as from independent projects. The projects do not have to be complete; presentations can represent any stage in the concept’s evolution, from proposal and literature …

Wright State University Celebration Of Research, Scholarship And Creative Activities Book Of Abstracts Friday, April 12, 2013, Wright State University Office Of Undergraduate Research And Stemm Activities

Celebration of Undergraduate & Graduate Research, Scholarship, and Creative Activities Materials

The student abstract booklet is a compilation of abstracts from students' oral and poster presentations at Wright State University's second annual Celebration of Research, Scholarship and Creative Activities on April 12, 2013.

The Santa Clara, 2013-04-11, Santa Clara University

The Santa Clara

No abstract provided.

The Santa Clara, 2013-04-04, Santa Clara University

The Santa Clara

No abstract provided.

Instrumental Music Ensemble At Tennessee State University: The Early Years (1912-1946), Reginald A. Mcdonald

The Journal of Tennessee State University

No abstract provided.

The Resilience Of One Tennessee State University Professor In Providing A Path To Excellence For Youths, Sean Daniels

The Journal of Tennessee State University

No abstract provided.

Multiscale Modeling Of Enzyme-Catalyzed Methanol Production By Particulate Methane Monooxygenase, Katherine K. Bearden

Doctoral Dissertations

In this work, the conversion of methane to methanol by the particulate Methane Monooxygenase (pMMO) enzyme is investigated using a multi-scale modeling approach. This enzyme participates in carbon cycling and aids in the removal of harmful atmospheric methane, converting it to methanol. The interaction between pMMO and a neighboring enzyme that is present in the same organism is studied, and the unknown pMMO active site is elucidated and tested for methane oxidation towards the production of methanol.

Fundamental knowledge of pMMO's mechanism is not fully understood. Understanding how this enzyme works in nature will provide information towards designing efficient synthetic …

Runoff Of Silvicultural Herbicides Applied Using Best Management Practices, Matthew W. Mcbroom, Jeff Louch, R Scott Beasley, Mingteh Chang, George G. Ice

Faculty Publications

Nine small (2.2 to 2.9 ha) and four large (70 to 135 ha) watersheds in East Texas, USA, were instrumented to compare herbicide runoff under different silvicultural systems with best management practices (BMPs). Two treatments were evaluated: conventional, with clearcutting, aerial herbicide site preparation, and hand-applied banded herbaceous release; and intensive, in which subsoiling, aerial fertilization, and a second-year aerial herbicide application were added. Herbicides were applied as operational tank mixes. The highest imazapyr concentration found in stream water was 39 mg L^‑1 during the first storm after application (23 days after treatment, DAT) and in-stream concentrations …