Engineering Commons^™

Numerical Simulation Of Terahertz Wave Interaction With Breast Cancer Tumor Tissue Sections, Abayomi Omotola Omolewu

Graduate Theses and Dissertations

This thesis presents numerical simulation of terahertz (THz) wave interaction with breast cancer tumor tissue sections. The obtained results are expressed in THz images of heterogeneous material that mimics the excised breast cancer tissue sections. The finite-element software package ANSYS High Frequency Structural Simulator (HFSS) was used in this work. HFSS is a full wave frequency domain three-dimensional (3D) electromagnetic simulation package. In this work, four breast cancer tissue models based on pathology images were simulated and images of the models were obtained at 1 THz. An incident Gaussian beam was raster scanned over tissue model configurations and the reflected …

Using Peptoids To Build Robust, Efficient Microarray Systems, Dhaval Sunil Shah

Graduate Theses and Dissertations

Recent studies have shown microarrays to be indispensable for various biological applications, allowing for high-throughput processing and screening of biological samples such as RNA, DNA, proteins and peptides using a small sample volume (< 1 µL). Peptoids (poly-N-substituted glycine oligomers) can be used as a substitute for antibodies as capture molecules, as well as coatings for slides in antibody microarrays. The ease of synthesis of peptoids, high customizability with desired bioactivity, and speed of synthesis allows us to build a diagnostic system with a large dynamic range that can detect biomolecules from a minimal sample size. In this study, peptoid-based antibody mimics are designed to have both structural and functional features similar to those of antibodies, including a stable constant region (scaffolding) and a variable region for protein recognition. Peptoids previously screened via combinatorial library synthesis to be specific to bind Mdm-2 (mouse double minute 2 homolog) and GST (gluthathione S-transferase), have been synthesized. The protein recognition peptoids have been conjugated to PEG (polyethylene glycol) molecules with modified end groups; an amine group on one end that allows for immobilization and orientation on the slide, and an azide group on the other end that will allow for attachment to the peptoid through “click chemistry”. The number of capture molecules printed on the slides can be increased by making the available surface area of the slide larger via coating with microspheres. We have determined that partially water soluble peptoids that are also helical, can self-assemble into microspheres. Sequences have been developed that can consistently produce uniform microsphere coatings on slides that increase the overall surface area. A high surface area corresponds to a higher number of binding sites, and therefore a more sensitive system. The work done has shown that slides may be successfully coated in order to potentially improve the detection system.

Deletion Of Nfnab In Thermoanaerobacterium Saccharolyticum And Its Effect On Metabolism, Jonathan Lo, Tianyong Zheng, Daniel G. Olson, Natalie Ruppertsberger, Shital Tripathi, Adam Guss, Lee Lynd

Dartmouth Scholarship

NfnAB catalyzes the reversible transfer of electrons from reduced ferredoxin and NADH to 2 NADP⁺. The NfnAB complex has been hypothesized to be the main enzyme for ferredoxin oxidization in strains of Thermoanaerobacterium saccharolyticum engineered for increased ethanol production. NfnAB complex activity was detectable in crude cell extracts of T. saccharolyticum. Activity was also detected using activity staining of native PAGE gels. The nfnAB gene was deleted in different strains of T. saccharolyticum to determine its effect on end product formation. In wild-type T. saccharolyticum, deletion of nfnAB resulted in a 46% increase in H₂ formation but …

Slides: Wrapping Up The Big Horn Adjudication: Lessons After 38 Years And 20,000 Claims, Ramsey L. Kropf

Innovations in Managing Western Water: New Approaches for Balancing Environmental, Social and Economic Outcomes (Martz Summer Conference, June 11-12)

Presenter: Ramsey L. Kropf, Deputy Solicitor for Water Resources, Office of the Solicitor, U.S. Department of the Interior

34 slides

Agenda: Innovations In Managing Western Water: New Approaches For Balancing Environmental, Social, And Economic Outcomes, University Of Colorado Boulder. Getches-Wilkinson Center For Natural Resources, Energy, And The Environment

Innovations in Managing Western Water: New Approaches for Balancing Environmental, Social and Economic Outcomes (Martz Summer Conference, June 11-12)

Many aspects of western water allocation and management are the product of independent and uncoordinated actions, several occurring a century or more ago. However, in this modern era of water scarcity, it is increasingly acknowledged that more coordinated and deliberate decision-making is necessary for effectively balancing environmental, social, and economic objectives. In recent years, a variety of forums, processes, and tools have emerged to better manage the connections between regions, sectors, and publics linked by shared water systems. In this event, we explore the cutting edge efforts, the latest points of contention, and the opportunities for further progress.

Slides: Moffat Collection System Project, Travis Bray

Innovations in Managing Western Water: New Approaches for Balancing Environmental, Social and Economic Outcomes (Martz Summer Conference, June 11-12)

Presenter: Travis Bray, Project Manager, Moffat Collection System Project, Denver Water

45 slides

Slides: Ag Water Sharing: Legal Challenges And Considerations, Peter D. Nichols

Innovations in Managing Western Water: New Approaches for Balancing Environmental, Social and Economic Outcomes (Martz Summer Conference, June 11-12)

Presenter: Peter D. Nichols, Esq., Partner, Berg, Hill, Greenleaf and Ruscitti, Boulder, CO

25 slides

Spectrally-Resolved Imaging Of The Transverse Modes In Multimode Vcsels, Stephan A. Misak, Dan G. Dugmore, Kirsten A. Middleton, Evan R. Hale, Kelly R. Farner, Kent D. Choquette, Paul O. Leisher

Rose-Hulman Undergraduate Research Publications

Vertical-cavity surface-emitting lasers (VCSELs) enable a range of applications such as data transmission, trace sensing, atomic clocks, and optical mice. For many of these applications, the output power and beam quality are both critical (i.e. high output power with good beam quality is desired). Multi-mode VCSELs offer much higher power than single-mode devices, but this comes at the expense of lower beam quality. Directly observing the resolved mode structure of multi-mode VCSELs would enable engineers to better understand the underlying physics and help them to develop multi-mode devices with improved beam quality. In this work, a low-cost, high-resolution (<3 >pm) …

Work Integrated Learning In Stem In Australian Universities: Final Report: Submitted To The Office Of The Chief Scientist, Daniel Edwards, Kate Perkins, Jacob Pearce, Jennifer Hong

Higher education research

The Australian Council for Educational Research (ACER) undertook this study for the Office of the Chief Scientist (OCS). It explores the practice and application of Work Integrated Learning (WIL) in STEM, with a particular focus on natural and physical sciences, information technology, and agriculture departments in Australian universities. The project involved a detailed ‘stocktake’ of WIL in practice in these disciplines, with collection of information by interview, survey instruments, consultation with stakeholders and literature reviews. Every university in Australia was visited as part of this project, with interviews and consultation sessions gathering insight from more than 120 academics and support …

Wireless Sensor Mesh Network For Irrigation Systems, Rosana Cheruvelil, Oswaldo Garcia, Nabil Haque, Justin Ignacio, Stanley Thomas

Electrical Engineering

Pepper Oak Farms, a company that grows their own olives for olive oil and has approximately 40,000 olive trees, is in need of an efficient way to monitor the soil and atmospheric conditions that are critical to the cultivation of their trees. The company at the moment only has two sensors and has to manually place the sensors at different locations to collect data on soil moisture and temperature. This is expensive, time consuming, and a lot of effort on the owner’s part to go out on the fields to collect this data. As a solution, the company would like …

Sports For Nathan, Delaney Bales, Joseph Garrett, Chris Harter

Mechanical Engineering

Nathan Cooper is a local boy with spinal muscular atrophy. He enjoys playing games and being active. One assistive device that Nathan uses is his Standing Dani, a motorized mobile stander. Nathan enjoys playing golf and kickball, and prior to this project he had no way of playing golf and no safe way of playing kickball. The purpose of this project was to design and build a golf attachment for Nathan’s Standing Dani that allows him to putt, and a kickball attachment that allows him to play kickball safely. First, the design team researched and defined the problem in terms …

The Influence Of Neuromusculoskeletal Model Calibration Method On Predicted Knee Contact Forces During Walking, Gil Serrancolí, Allison Kinney, Benjamin J. Fregly, Josep M. Font-Llagunes

Mechanical and Aerospace Engineering Faculty Publications

This study explored the influence of three model calibration methods on predicted knee contact and leg muscle forces during walking. Static optimization was used to calculate muscle activations for all three methods. Approach A used muscle-tendon model parameter values (i.e., optimal muscle fiber lengths and tendon slack lengths) taken directly from literature. Approach B used a simple algorithm to calibrate muscle-tendon model parameter values such that each muscle operated within the ascending region of its normalized force-length curve. Approach C used a novel two-level optimization procedure to calibrate muscle-tendon, moment arm, and neural control model parameter values while simultaneously predicting …

Multiphysics Modeling To Enhance Understanding Of Microwave Heating Of Food In Domestic Ovens, Jiajia Chen

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Nonuniform heating is the biggest issue in the microwave heating of prepared meals. Multiphysics based models are promising tools to improve microwave heating uniformity by properly designing the food product. However, limited availability of accurate temperature-dependent material properties, inadequate model prediction accuracy, and high computational power and complexity in model development are three gaps that greatly limited the application of these models in the food industry.

To fill in the gaps, firstly, we developed a multitemperature calibration protocol to measure temperature-dependent dielectric properties (dielectric constant and loss factor). The temperature-dependent dielectric and thermal (thermal conductivity and specific heat capacity) properties …

Photosynthetic Performance And Anti-Oxidative Response Of Cornus Controversa Seedlings Under Cadmium And Lead Stress, Xinyu Huang, Yonglei Jiang, Xiaomao Cheng, Lilan Deng, Xuncheng Liu

Faculty Publications

The photosynthetic efficiency of Cornus controversa leaves was decreased significantly under Cd treatment while it was not affected by Pb exposure. Cd decreased while Pb treatment increased the chlorophyll contents of Cornus controversa leaves. Furthermore, the peroxidase (GPX) activities were decreased after Cd treatment while elevated by Pb exposure in Cornus controvera seedlings. In addition, both Cd and Pb exposures increased the malondialdehyde (MDA) and proline contents and elevated the superoxide dismutase (SOD) activities of Cornus controvera seedlings. Collectively, these results indicated that Cornus controversa may be more tolerant to Pb than Cd toxicity. This finding will contribute to the …

Swift Fox Abundance Along The Heartland Expressway Corridor In Nebraska, Marc Albrecht

Nebraska Department of Transportation: Research Reports

No abstract provided.

Predictive Modeling Of Oxygen Transmission Through Micro-Perforations For Packaging Applications, Ayman Abdellatief, Bruce A. Welt, Jason Butler, Eric Mclamore, Arthur Teixeira, Sanjay Shukla

Journal of Applied Packaging Research

Methods for creating precise perforations in respiring produce packaging are being increasingly adopted. Knowledge of oxygen transfer through perforated packaging and oxygen distribution in packages is necessary for successful packaging design of fresh produce. An approach to modeling perforated packaging performance was developed using a cylindrical chamber with precision perforations using Fick’s second law. The model was simulated using two techniques including Finite Element Method (FEM) using commercially available software and Finite Volume Method (FVM) through programming. Perforations were approximated as a source term in the second method. Both simulation techniques showed trends similar to experimental data.

Cofactor Specificity Of The Bifunctional Alcohol And Aldehyde Dehydrogenase (Adhe) In Wild-Type And Mutant Clostridium Thermocellum And Thermoanaerobacterium Saccharolyticum, Tianyong Zheng, Daniel G. Olson, Liang Tian, Yannick J. Bomble, Michael Himmel, Jonathan Lo, Shuen Hon, A. Joe Shaw, Johannes P. Van Dijken, Lee Lynd

Dartmouth Scholarship

Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are thermophilic bacteria that have been engineered to produce ethanol from the cellulose and hemicellulose fractions of biomass, respectively. Although engineered strains of T. saccharolyticum produce ethanol with a yield of 90% of the theoretical maximum, engineered strains of C. thermocellum produce ethanol at lower yields (∼50% of the theoretical maximum). In the course of engineering these strains, a number of mutations have been discovered in their adhE genes, which encode both alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) enzymes. To understand the effects of these mutations, the adhE genes from six strains of C. …

Three-Dimensional Ideal Gas Reference State Based Energy Function, Avdesh Mishra

LSU New Orleans Theses and Dissertations

Energy functions are found to be a key of protein structure prediction. In this work, we propose a novel 3-dimensional energy function based on hydrophobic-hydrophilic properties of amino acid where we consider at least three different possible interaction of amino acid in a 3-dimensional sphere categorized as hydrophilic versus hydrophilic, hydrophobic versus hydrophobic and hydrophobic versus hydrophilic. Each of these interactions are governed by a 3-dimensional parameter alpha used to model the interaction and 3-dimensional parameter beta used to model weight of contribution. We use Genetic Algorithm (GA) to optimize the value of alpha, beta and Z-score. We obtain three …

Photocatalytic Mineralization Of Phenol On Fluidized Titanium Oxide-Coated Silica Gel, Guillermo J. Rincon

LSU New Orleans Theses and Dissertations

A bench-scale tubular reactor with recirculation was built in order to study the efficiency of the photocatalytic oxidation of phenol on fluidized titanium oxide-coated silica gel beads. A UV-C lamp placed along the central vertical axes of the reactor was used as source of photons. A bed of silica gel beads was fluidized by means of fluid recirculation and forced to follow upward helical flow around the lamp. Anatase was successfully synthetized on silica gel particles of average diameters 224, 357 and 461 µm, as confirmed by scanning electron micrographs, through a sol-gel technique using a titanium (iv)isopropoxide / hydrochloric …

Bio-Inspired Structural Colors Produced Via Self-Assembly Of Synthetic Melanin Nanoparticles, Ming Xiao, Yiwen Li, Michael C. Allen, Dimitri D. Deheyn, Xiujun Yue, Jiuzhou Zhao, Nathan C. Gianneschi, Matthew D. Shawkey, Ali Dhinojwala

Polymer Science Faculty Research

Structural colors arising from interactions of light with submicron scale periodic structures have been found in many species across all taxa, serving multiple biological functions including sexual signaling, camouflage, and aposematism. Directly inspired by the extensive use of self-assembled melanosomes to produce colors in avian feathers, we set out to synthesize and assemble polydopamine-based synthetic melanin nanoparticles in an effort to fabricate colored films. We have quantitatively demonstrated that synthetic melanin nanoparticles have a high refractive index and broad absorption spanning across the UV–visible range, similar to natural melanins. Utilizing a thin-film interference model, we demonstrated the coloration mechanism of …

3d Printing In Healthcare, Caleb Branch

The Review: A Journal of Undergraduate Student Research

Technology is everywhere. Technology surrounds every aspect of 21^st century life. It is in the cell phones we use, the cars we drive, and even the food we eat. A large portion of modern technology used is taken for granted and overlooked. Despite this, some technology fields continue to grow. Biomedical engineering, specifically 3D printing’s applications to healthcare, has been often overlooked until. Regardless of its status in the mainstream, 3D printing is prosperous in healthcare and its future looks bright. This piece analyzes 3D printing in healthcare. It hones in on the finer details of each specific topic, …

Biofilm-Related Materials As Total N-Nitrosamine (Tono) Precursors And Hydroxylamine-Based Interferences In Tono And N-Nitrosodimethylamine (Ndma) Measurements, David Allen Meints

Graduate Theses and Dissertations

In this study, a chemiluminescence-based total N-nitrosamine (TONO) assay was adapted to include a solid-phase extraction (SPE) step to assess the role of biologically derived materials as N-nitrosamine precursors. Methanol was determined to be a suitable solvent for the SPE-TONO assay, with N-nitrosodimethylamine (NDMA) losses estimated to be 30% by SPE alone and an additional 15% from concentration of the methanol extracts by N2-gas blowdown. Three biofilm-derived materials - poly-N-acetylglucosamine (PNAG), Pseudomonas aeruginosa, and tryptophan - were individually chloraminated and dose-response relationships were observed with the SPE-TONO assay, indicating biofilm are potential N-nitrosamine precursors. The role of hydroxylamine - a …

Synthesis, Functionalization, And Characterization Of Metallic And Organic Nanoparticles For Biomedical Applications, Christopher Brian Sylvester

Honors Capstones

No abstract provided.

Design, Programming, And User-Experience, Kaila G. Manca

Honors Scholar Theses

This thesis is a culmination of my individualized major in Human-Computer Interaction. As such, it showcases my knowledge of design, computer engineering, user-experience research, and puts into practice my background in psychology, com- munications, and neuroscience.

I provided full-service design and development for a web application to be used by the Digital Media and Design Department and their students.This process involved several iterations of user-experience research, testing, concepting, branding and strategy, ideation, and design. It lead to two products.

The first product is full-scale development and optimization of the web appli- cation.The web application adheres to best practices. It was …

Novel Bioinformatic Approaches For Analyzing Next-Generation Sequencing Data, Yan Peng

Dissertations

In general, DNA reconstruction is deemed as the key of molecular biology since it makes people realize how genotype affects phenotypes. The DNA sequencing technology emerged exactly towards this and has greatly promoted molecular biology’s development. The traditional method, "Sanger," is effective but extremely expensive on a cost-per-base basis. This shortcoming of Sanger method leads to the rapid development of next-generation sequencing technologies. The NGS technologies are widely used by virtue of their low-cost, high-throughput, and fast nature. However, they still face major drawbacks such as huge amounts of data as well as relatively short read length compared with traditional …

Selection Methods For Genetically-Modified T Cells: In Support Of Translational Therapy, David Rushworth

Dissertations and Theses (Open Access)

T cells are blood cells which organize the immune system of the host. These cells are necessary for the host to respond appropriately to threats from foreign organisms and cancerous growth. However, in the case of certain infections and cancer, T cells are unable to respond appropriately to a threat and establish immunity. This leads to disease when the infection or cancer is not sufficiently eliminated. On the other hand, T cells can lack tolerance for healthy tissue and perceive healthy tissue as infected. The ensuing over-reactive immune response also leads to disease. A delicate balance must exist between immunity …

Manipulating The Mass Distribution Of A Golf Putter, Paul J. Hessler Jr.

Senior Honors Projects

Putting may appear to be the easiest but is actually the most technically challenging part of the game of golf. The ideal putting stroke will remain parallel to its desired trajectory both in the reverse and forward direction when the putter head is within six inches of the ball. Deviation from this concept will cause a cut or sidespin on the ball that will affect the path the ball will travel.

Club design plays a large part in how well a player will be able to achieve a straight back and straight through club head path near impact; specifically the …

In Vitro Studies Of Gold And Gold Silica Nanoparticle Radiosensitization With Kilovoltage X-Rays, Gregory Colarch

UNLV Theses, Dissertations, Professional Papers, and Capstones

Technological advances in the ability to construct and manipulate nanoscale particles have opened up the possibility of using solid metallic nanoparticles and mixed metal nanoshells as a means to increase dose enhancement and treatment efficacy to tumors. In order for nanoparticles to be an effective form of treatment, they must be delivered to tumors in sufficient concentrations so that there is a dose enhancement factor due to ionizing radiation, as well as being essentially non-toxic to healthy cells. Gold nanoparticles and silica-gold nanoshells fit these requirements. Gold has a high atomic number (Z=79), which gives a larger cross section for …

Developing An Application For Evolutionary Search For Computational Models Of Cellular Development, Nicolas Scott Cornia

Boise State University Theses and Dissertations

VPEvolve is a free and open source application that utilizes a Visual Programming Environment (VPE) for the setup of the Genetic Algorithm (GA), for optimization of computational models. Specifically, the User Interface uses connected glyphs to represent the genetic operators of mutation, reproduction, fitness and selection. These glyphs give the user an intuitive way to set the parameters for the GA, and better visualization of the population's flow through these operators.

VPEvolve is currently being developed alongside research being done in Biocomputing to create models of cellular regeneration based on the regenerative properties of Planaria or flatworms. Since these models …

Mapping Regional Inundation With Spaceborne L-Band Sar, Bruce Chapman, Kyle Macdonald, Masanobu Shimada, Ake Rosenqvist, Ronny Schroeder, Laura Hess

Publications and Research

Shortly after the launch of ALOS PALSAR L-band SAR by the Japan Space Exploration Agency (JAXA), a program to develop an Earth Science Data Record (ESDR) for inundated wetlands was funded by NASA. Using established methodologies, extensive multi-temporal L-band ALOS ScanSAR data acquired bi-monthly by the PALSAR instrument onboard ALOS were used to classify the inundation state for South America for delivery as a component of this Inundated Wetlands ESDR (IW-ESDR) and in collaboration with JAXA’s ALOS Kyoto and Carbon Initiative science programme. We describe these methodologies and the final classification of the inundation state, then compared this with results …