Digital Commons Network^™

Construction Of Recombinant E Coli. Of Producing Β-Phenylalanine Aminomutase And Optimization Of Fermentation Conditions, Ge Fei, Tang Yao, Zhu Longbao, Li Yiwen, Duan Zhenchao, Ma Qisen, Tao Yugui

Food and Machinery

The genetic engineering strain with recombinant plasmid pET-28a-pam was expressed in E.coli BL21. The fermentation conditions of initial pH, shaking speed, inoculation time and fermentation time were optimized with the PAM activity by using single factor. The results showed that the optimal fermentation conditions were initial pH 7.0, 200 r/min, the tenth hour broth and liquid volume 50 mL/500 mL, fermented for 24 h. Furthermore, the three factors were optimized by Box-Behnken design, and the results showed that inoculation time, initial pH and fermentation time are main affecting factors and their optimal levels are 12.8 h, 6.7 and 25.2 h. …

Effect Of Nano-Sio2 Modified Ldpe Film On Postharvest Quality Of Gonggan, Song Mubo, Fang Fang, Luo Zishen, Li Dong, Chen Zhenlin, Xuan Liting, Shuai Liang, Duan Zhenhua

Food and Machinery

In order to study the effect of nano-SiO₂ modified low density polyethylene (LDPE) film on Gonggan storage, the influences of unpacked, LDPE film and nano-SiO₂ LDPE film packaging treatments on postharvest quality of Gonggan were investigated. The results showed that both of the packing materials could improve the postharvest quality of Gonggan, and nano-SiO₂ LDPE film was better in slowing down the increase of the decay rate and weight loss ratio, and the decrease of firmness. Moreover, the nano-SiO₂ LDPE film was found to be better in inhibited the accumulation of malonaldehyde (MDA), and maintained higher …

Application Of A New Type Of Fluorine Free Refrigerant Hcr22 In Food Refrigeration System, Liu Jinguang, Xiong Xubo, Wang Shiqing, Luan Mingchuan, Zhang Yan, Jiang Wenli

Food and Machinery

In order to explore the feasibility of the application of new fluorine free refrigerant in the existing refrigeration system, a traditional one based on R600a refrigeration, a testing equipment of new refrigeration system was designed. This new testing equipment was dominated by a cooling equipment, temperature tester and pressure detector. In this study, the refrigerant R600a replaced by a new type of fluorine free refrigerant HCR22, and the coefficient of performance and cold storage capacity were taken as indexes, and conducted an experiment for 12 h, the refrigerating capacity of two refrigerants in different quantity of charging ratio was studied. …

Extraction And Antioxidant Activity In Vitro Of Flavonoids In Cold Pressed Cake Of Zanthoxylum Armatum Dc. Prodr., Wang Chunxia, Pu Biao, Jiang Yan, Fu Benning

Food and Machinery

Response surface methodology was used to optimize the microwave- assisted extraction of flavonoids from cold pressed cake in Zanthoxylum armatum DC. Prodr， and the antioxidant activity in vitro of flavonoids were analyzed. The results showed that the optimum extraction process of flavonoids were： microwave power 506 W, microwave treatment time 256 seconds, ethanol concentration 75%, ratio of liquid to material 501. Under this condition, extraction ratio of flavonoids was 0.40%±0.04% ( in terms of rutin). The reducing power, the hydroxyl free radicals, DPPH free radicals, scavenging capacity of flavonoid extracts were increased gradually with the increasing of the mass concentration, …

Study On Isolation And Antioxidant Activity Of The Polysaccharide From Ganoderma Lucidum, Xu Xuefeng, Li Guijuan, Yan Hao, Zhang Yu, Zhang Jingchang

Food and Machinery

Isolation, purification and antioxidant activity in vitro of polysaccharide from Ganoderma lucidum were studied. Polysac-charide was extracted from G. lucidum, and three fractions were isolated through ion exchange chromatography and allyl dextran gel chromatography from them, including GLPa-2, GLPb-1, and GLPc. The weight-average molecular weight was detected by gel permeation chromatographic, and then monosaccharide composition was detected by gas chromatography. The results were described as follows. The component parts of polysaccharides were composed of glucose, arabinose, xylose and mannose, with different containing rates, and the average molecular weight of GLPa-2, GLPb-1, GLPc was detected to be 3.65×10⁵ Da, 3.87×10 …

Effect Of Total Flavonoids From Perennial Lablab Sp. Hull On Immune Function In Mice, Cao Baiying, Jiang Xiujuan, Qi Yingxin, Chang Youquan

Food and Machinery

The effect of total flavonoids from Perennial Lablab sp. Hull(FPLH) on the immune function and antioxidant activity in vivo were studied. With index of immune organ, spleen lymphocyte proliferation function, phagocytic activity of macrophages, cytokine levels in serum(IFN-γ and IL-4), the activity of alkaline phosphatase in serum, the activity of SOD, GSH-Px and the level of MDA in serum as the evaluated indices, the immune function and antioxidant activities of FPLH in mice was studied. The results showed that the thymus index and spleen index of FPLH was higher than the control group by 12.34% and 8.67% respectively; the spleen …

Ultrasonic Enhancement Of Tartary Buckwheat Germination And The Production Of Nutrients In Sprouts, Wang Shunmin, Wang Jianfei

Food and Machinery

Tartary buckwheat (Fagopyrum tataricum) seeds were treated using ultrasonic at different power and time and temperature. The exposed grains were germinated, then harvested after incubation on the 2rd, 4th, and 6th days. The effects of ultrasonic on the germination rate of the tartary buckwheat seeds and some nutrient compounds in the sprouts were detected, and the DPPH radical-scavenging activities of the seedlings were investigated. The results showed that with treatment of 280 W ultrasonic, at 20 ℃ for 35 min, the highest rates of initial (88.0%) and final (100.0%) germination were obtained after incubation. With the treatment of 240 W …

Optimization On Extrusion Process Ofaloe-Maize Blend By Linear Interpolation Method, Wei Hongyu, Luo Shaoming, Zhu Lixue, Zhou Yumei

Food and Machinery

Aiming at the problem of low efficiency of by-product in aloe processing, the aloe leaves were dried，crushed and mixed with maize flour, and then were extruded to make aloe-maize health food. Single factor and five factors four levels orthogonal test were used to study the influence of process parameters on characteristics of the extruded products. Linear interpolation method was used to evaluate the products characteristics, and the process parameters were optimized: the feeding rate is 30 r/min, the screw speed is 115 r/min, the water content is 14%, the aloe content is 4% and the extrusion temperature is 150 ℃

Study On Technology Of Coagulated By Fermented Soybean Processing Waste Water, Xie Linglai, Zhao Liangzhong, Yin Lebin, Chen Chuqi, Zhou Juan, Zhu Dan

Food and Machinery

Fermented soybean processing waste water was used as coagulant to produce tofu. On the basis of single factor experiments, the total acid of fermented soybean processing waste water, amount of fermented soybean processing waste water and the temperature of pointing soybean milk were main factors, taking the sensory evaluation score and springiness, and the coagulation conditions were optimized through response surface methodology. Results showed that the optimum coagulation parameters were as follows: the total acid of fermented soybean processing waste water 5.3 g/kg, the amount of fermented soybean processing waste water 29% and the temperature of pointing soymilk 76 ℃. …

Optimization Of Technological Conditions For Enzymatic Hydr Olysis Of Sweet Corn Pulp, Lu Manxia, Zhang Ling, Lin Zhisang, Wang Zeqi, Huang Xiaocui, Xie Huxi

Food and Machinery

The sweet corn juice was prepared by using double enzymes method, i.e. catalyzed by medium temperature α-amylase and saccharifying enzyme. The soluble solid matter content was analyzed as the basis of evaluation. The liquefaction process was investigated in solid-liquid ratio, medium temperature α-amylase dosage, enzymatic hydrolysis temperature and time on the amylolysis effect, and then the effects of the amount of saccharifying enzyme, saccharification temperature and time on the enzyme solution were also studied. On the basis of single factor experiments, the optimum technological conditions of double enzyme hydrolysis for sweet corn were optimized by orthogonal experiment. The results showed …

The Application Of Phosphate In Wheat Based Products, Wang Li, Chen Min, Zhao Junfeng, Qian Haifeng, Zhang Hui, Qi Xiguang

Food and Machinery

Phosphate is a kind of food additive, which can improve the quality of food. It is widely used in all fields of food industry and plays an important role in the production of wheat based products. In this paper, the basic characteristics of phosphate were introduced, and the application and mechanism of phosphates in flour products were also summarized. Furthermore, the problems of its application and the corresponding suggestions were proposed.

Research Progress Of Inonotusobliquus Polysaccharides, Chang Chen, Bao Yihong

Food and Machinery

Inonotus obliquus polysaccharide is one of the major constituents of active components in Inonotus obliquus, which has important function on antioxidant, anti-tumor etc. and is one kind of potential health ingredients. Study the progresses of Inonotus obliquus polysaccharide, including the extraction, isolation, purification, identification, bioactivity and composition, and offer the futher researchs and prospects of Inonotus obliquus polysaccharide.

The Origin Of Ecological Liquor-Making And Its Industrial Thought, Yu Yougui, Zeng Hao

Food and Machinery

The ecological wine products as the center have been provided, and the new model of circular economy have also been built in the industry of ecological liquor-making. Ecological liquor-making embodied in the pursuit of harmony of economic, social and ecological benefits to achieve coordinated development between the human and the nature environment, between the brewing industry and natural environment, and between social and natural environment. It was contained that the harmony thought of Taoist culture, Marx's thought of ecological ethics, the whole idea of system theory, the sustainable development's thinking of scientific development views, and the beautiful Chinese thought of …

Analysis Of Current Situation And Development Tendency Regarding Marine Food Utilization And Processing Industry, Zhang Rongbin, Tang Xu

Food and Machinery

In this paper, it was elaborated the basic situation of marine biology resources and potential of sea food development in China, and the development status of marine food industry was also analyzed. It was summarized that the product category is simple and mainly focuses on frozen seafood. Moreover, the current products feature is of low added-value and utilization rate. Therefore, we raised kinds of restraining factors of seafood industry development in China, and discussed the future trend of seafood industry development in order to promote the marine livelihood and economy.

Complexity As A Narrative, Giacomo Pala

Journal of Applied Sciences and Arts

In this paper an attempt is made to discuss how the computer has imposed new conventions to architecture over the last thirty years. The paper’s aim is to discuss how the introduction of digital media has forced architecture to find ways to deal with new technologies and to develop new disciplinary meanings. First, the paper addresses the question of complexity and chaos in the postmodern discussion by the reading of Lyotard’s theories. Therefore, the essay pinpoints attempts of developing new conceptions of “complexity” in architecture derived from the post-modern sciences of indetermination. Interengsly enough, this was doable because both these …

Spatial Control Of Cell Polarity And Cell Growth By Conserved Ndr Kinase Orb6, Illyce Nunez

Open Access Dissertations

During many cellular processes, such as cell morphogenesis, migration, and asymmetric cell division, eukaryotic cells modulate their growth and polarity. Understanding the molecular mechanisms that govern polarized cell growth has the potential to reveal important insights into diseases such as neurodegeneration, heart disease, and cancer. The model organism fission yeast Schizosaccharomyces pombe, with its cylindrical shape and bipolar growth zones, enables straightforward evaluation of changes in growth and polarity upon genetic or pharmacological manipulation. In addition, S. pombe has a characteristic response to nutrient starvation, which causes cells to alter their morphology by dividing at a shorter cell length and …

Wastewater Indicators And Exfiltration Sources In Wilson Creek, Springfield, Missouri: Final Report, Marc R. Owen, Teresa M. Carroll

OEWRI Technical Reports

No abstract provided.

Imperfectly Known Or Socially Constructed? What Is Truth Again?, Scott C. Campanario, Paul R. Yost

SPU Works

Contemporary psychology is once again at an inflection point with regard to its philosophical foundation. In this paper, we evaluate two prominent philosophies of science within the field of psychology—post-positivism and social constructionism—that are logically incompatible but often treated as equally valid by theorists, researchers, and practitioners. We discuss what each philosophy of science offers in terms of ontology, epistemology, and pragmatic justifications using the structure of a proposed argument, counterargument, and rebuttal. From this evaluation, we contend that post-positivism is a logically preferable philosophy of science for both the progress of collective knowledge and the sustainability of psychology as …

Sustained Epidermal Powder Drug Delivery Via Skin Microchannels, Yan Cao, Prateek Kakar, Md. Nazir Hossen, Mei X. Wu, Xinyuan Chen

Biomedical and Pharmaceutical Sciences Faculty Publications

Transdermal delivery of hydrophilic drugs is challenging. This study presents a novel sustained epidermal powder delivery technology (sEPD) for safe, efficient, and sustained delivery of hydrophilic drugs across the skin. sEPD is based on coating powder drugs into high-aspect-ratio, micro-coating channels (MCCs) followed by topical application of powder drug-coated array patches onto ablative fractional laser-generated skin MCs to deliver drugs into the skin. We found sEPD could efficiently deliver chemical drugs without excipients and biologics drugs in the presence of sugar excipients into the skin with a duration of ~ 12 h. Interestingly the sEPD significantly improved zidovudine bioavailability by …

Biology Grad Student Authors Article On Shigella Dysenteriae Pathogenesis, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Usft Scholar Commons Annual Report: Fy 2015-2016, Chelsea Johnston, Carol Ann Davis, Jason Boczar

Digital Commons @ USF Publications

In fall 2011, the Academic Resources Unit of the University of South Florida (USF) Library launched Scholar Commons, an institutional repository (IR) intended to serve as a virtual showcase for USF’s research and creative energies. Scholar Commons provides digital preservation and free open access (OA) to scholarly works produced by the USF Community. Our vision is to become a vehicle to drive research communication across academic disciplines.

Through our dedication to and outreach of scholarly communications, we have generated many positive outcomes. This report chronicles our initiatives during the 2015/2016 fiscal year (FY), and includes:

• A review of the purpose, …

Biology Alum Is Hospitalist At Dearborn County Hospital, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Examining Constructs Of The Health Belief Model As Predictors Of Haitian Men's Intention Regarding Prostate Cancer Screening, Jhonii Price Louis Ii

Dissertations

The purpose of this study was to determine which of these selected constructs of the Health Belief Model (perceived susceptibility, perceived benefits, and perceived barriers) are predictors to the intention of Haitian men regarding prostate cancer screening. Other modifying variables were also considered as predictors to the outcome variable..

Migration And The Post-Fledging Period: Examining Two Critical Stages Of The Cerulean Warbler Life Cycle, Douglas William Raybuck

Student Theses and Dissertations

To reverse declining population trends for migratory birds, a full annual and life cycle conservation approach is necessary. However, two life history stages of relatively high mortality—the migratory and the post-fledging periods—are not well-understood for most declining bird species, including the Cerulean Warbler (Setophaga cerulea), a declining species considered vulnerable to extinction. Here, I explored the possibility of using geolocators to obtain migration data for Cerulean Warblers and examined potential negative effects of applying these devices to this species, finding no effects during the breeding season of deployment but lowered annual return rates compared with color-banded control counterparts. I also …

Cover Crops And Tillage Management For Enhanced Sustainability In Corn/Soybean Production In The Mississippi Delta Region Of Arkansas, David Scott Patton

Student Theses and Dissertations

A field experiment was established during 2014 in Northeast Arkansas to evaluate the benefits of cover crop and tillage management for enhanced yield and soil quality. Cover crop management included cereal rye, wheat and crimson clover, and a winter fallow. Tillage management included tillage and no-tillage. In the 2015 and 2016 seasons yield was not significantly changed by different tillage treatments. Cover crop out yielded no cover crop at the A-State site in the 2016 season. Organic matter and active carbon were significantly influenced by tillage treatments. Physical and biological properties were significantly increased with cover crops compared with no …

Effects Of Exposure To Quantum Dots In The External And Internal Environments Of Organisms, Kenton Layne Leigh

Student Theses and Dissertations

The use of nanotechnology in research and consumer products is increasing, but there are few studies regarding the safety of nanoparticles (NPs). NPs’ small size and increased surface area allow for unique physical and chemical properties not seen in bulk counterparts of the same material. Thus, NP interactions within environmental and biological matrices are not well understood. Specifically, quantum dots (QDs) have garnered increased attention due to their superior optical properties. However, little research on the effects of QDs on the environment is available. The studies described herein provide data regarding interactions of QDs in aquatic and terrestrial environments using …

The Hidden Morbidity Of Cancer: An Exploratory Study Of Burden In Caregivers Of Cancer Patients With Brain Metastases, Marlon Garzo Saria

Dissertations

The aim of this dissertation was to explore the antecedents and outcomes of caregiver burden within the context of brain metastases using the Comprehensive Health Seeking and Coping Paradigm as a framework to describe the highly interactive iii relationship among the variables. The first paper derived from this dissertation is a literature review that describes the challenges of caring for a patient with brain metastases and highlights the implications for healthcare professionals. The second paper presents the analysis of the relationships between caregiver burden and the affective disorders anxiety and depression. It describes the higher risk of screening positive for …

Sodium-Myoinositol Cotransporter-1, Smit1, Mediates The Production Of Reactive Oxygen Species Induced By Hyperglycemia In The Heart, Anne Van Steenbergen, Magali Balteau, Audrey Ginion, Laura Ferté, Sylvain Battault, Christophe De Meester De Ravenstein, Jean-Luc Balligand, Evangelos-Panagiotis Daskalopoulos, Patrick Gilon, Florin Despa, Sanda Despa, Jean-Louis Vanoverschelde, Sandrine Horman, Hermann Koepsell, Gerard Berry, Louis Hue, Luc Bertrand, Christophe Beauloye

Pharmacology and Nutritional Sciences Faculty Publications

Hyperglycemia (HG) stimulates the production of reactive oxygen species in the heart through activation of NADPH oxidase 2 (NOX2). This production is independent of glucose metabolism but requires sodium/glucose cotransporters (SGLT). Seven SGLT isoforms (SGLT1 to 6 and sodium-myoinositol cotransporter-1, SMIT1) are known, although their expression and function in the heart remain elusive. We investigated these 7 isoforms and found that only SGLT1 and SMIT1 were expressed in mouse, rat and human hearts. In cardiomyocytes, galactose (transported through SGLT1) did not activate NOX2. Accordingly, SGLT1 deficiency did not prevent HG-induced NOX2 activation, ruling it out in the cellular response to …

Perceptions Of The Arkansas Association Of Chiefs Of Police Regarding Preferred Educational Curricula For Law Enforcement Training In Higher Education, Dawn Deneice Phillips

Student Theses and Dissertations

The purpose of this research project was to examine perceptions of Arkansas Chiefs of Police (AACP) regarding preferred curricula for law enforcement training in higher education. Respondents were asked if national core curricula, mandated for Law Enforcement Training Academies located on colleges and universities would, improve police professionalism; serve to legitimize the police professionalism; improve police productivity; and, reduce incidents of police misconduct. Respondents were then asked what curricula should be utilized to best prepare law enforcement personnel. Finally, quantitative analysis was performed to determine if a relationship exist between curricula perceptions and the independent variables of experience, education, region, …

Discovering Similarities, Larry Burgess

Materials from the University of Dayton Magazine Website

Samantha Monendob (from left), first-year pre-med major from Kalamazoo, Michigan, Ben Bratton, first-year computer science major from Ann Arbor, Michigan, and Tijaun Kelly, sophomore psychology major from Chicago, learn about the evolutionary relationships of animal skeletons in a biology lab in the Science Center. Here they compare the bones of a large bird's skeleton with other animal bones.