Digital Commons Network^™

The Effect Of Capsicum Frutescens-L To Transient Receptor Potential Vinaloid-1, Toll Like Receptors (Tlr-4) And Interleukin 1 Beta (Il-1β) On Periodontitis, Jenny Sunariani, Latief Mooduto, Yuliati

Journal of Dentomaxillofacial Science

No abstract provided.

The Frequency Of Wearing Protective Eyewear And Face Shield At Educational Hospital And Public Service Hospital In Makassar, Rasmidar Samad, Ummul Rawiyah, Cisilia Septiany

Journal of Dentomaxillofacial Science

No abstract provided.

Effect Of Mangrove Leaves Extract (Avicennia Marina) Concentration To Streptococcus Mutans And Candida Albicans Growth, Mohammad Dharmautama, Richard Tetelepta, Muhammad Ikbal, Andi Ea. Warti

Journal of Dentomaxillofacial Science

No abstract provided.

Informal, Inquisitorial, And Accurate: An Empirical Look At A Problem-Solving Housing Court, Jessica K. Steinberg

GW Law Faculty Publications & Other Works

Substantive justice is often seen as elusive in courts dominated by low-income individuals. Complex court rules, coupled with pervasive lack of counsel, can make it difficult for the traditional adversary process to identify and redress legitimate grievances. This Article takes on the social problem of substandard housing and examines whether inquisitorial procedure has the potential to produce accurate outcomes in a tribunal dominated by the unrepresented.

Relying on in-court observations of nearly 300 hearings, and a longitudinal review of nearly 75 cases, this Article surfaces the regularized procedures utilized by a purported “problem-solving” housing court, and theorizes that the inquisitorial …

A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi

Turkish Journal of Veterinary & Animal Sciences

Ticks are the main hematophagous arthropods and obligatory ectoparasites that are considered a vector of serious pathogens for animals and humans. The aims of the present survey were to assess the prevalence and diversity of ticks in cattle in Southeast Iran and to determine Coxiella burnetii in the ticks. From May 2014 to April 2015, 583 ticks were collected randomly from 257 cattle. Nested trans-PCR was performed to detect C. burnetii. The overall prevalence of hard tick infestation on cattle was 56.8%. In total, seven different species were identified: Rhipicephalus sanguineus sensu lato (32.6%), Hyalomma anatolicum (28.8%), Hyalomma excavatum (19%), …

Paleodemographic And Biochemical Analysis Of Urbanization, Famine, And Mortality, Brittany S. Walter

Theses and Dissertations

Urbanization is a transitional period often associated with deteriorating population health and increased mortality, as the rapid increase of population density in urban centers facilitates the transmission of infectious diseases, unsanitary living conditions, and precarious food supplies. Research on the transition to an urban environment in the past offers a temporal depth to our understanding of the consequences of urbanization that cannot be accomplished through examination of contemporary populations. This project integrates paleodemographic (hazard analysis) and biochemical (stable isotope analysis) approaches to examine the health and diet of inhabitants in late medieval England (c. 1120-1539 CE), specifically the relationship between …

Analysis Of Food Exposures In Foodborne Disease Outbreaks, Alecia T. Alianell

Theses and Dissertations

Foodborne illness remains a serious public health problem in the United States in general as well as South Carolina in particular. Obtaining good food ingestion histories as well as possible risky environmental exposures is one of the earliest, most important tasks to complete in any foodborne outbreak investigations. Because time is of the essence in investigations, we have evaluated a rarely used biostatistical method, Random Forests, to data obtained from DHEC. Random Forests has the potential to facilitate more rapid identification of foods or environmental exposures that may be associated with outbreaks. We also examined previous cases of salmonellosis using …

Tuberculosis Screening In New Healthcare Employees: A Comparison Of Quantiferon®-Tb Gold In-Tube Test And Tuberculin Skin Test, Mary C. Giovannetti

Theses and Dissertations

Background: Streamlining onboarding processes for new hires to maximize efficiency and reduce costs while meeting regulatory requirements is a constant challenge for healthcare systems’ Employee Health staff. Health screening is a required step and includes obtaining a detailed health history, tuberculosis screening, drug screens, immunizations, fit for duty examinations, obtaining medical records, clarification of disability accommodations, pre-work screens, and other tests which are time consuming and result in delays in hire dates. Faced with a high volume of potential new employee hires a major southeast healthcare system was concerned about delays in new hire start dates. The two-step tuberculin skin …

Investigating Novel Methods Of Interaction With Pharmaceutically Relevant Enzymes, Cory T. Reidl

Dissertations

Metalloproteins requiring one or more metal ions for normal function make up 30% of all known proteins, and many critical biological pathways contain at least one metallo-enzyme. Di-nuclear metallo-proteins constitute a large class of these proteins yet we currently lack effective methods of inhibiting these enzymes for the development of new medical therapies, particularly for the discovery of new antibiotics. Our work has focused on developing novel functionalities that selectively interact with di-nuclear catalytic centers, and we are targeting three separate di-zinc-metallo-enzymes that are unique to bacteria and play key roles in their growth and development. These enzymes are DapE, …

Vector Competence Of Aedes Sierrensis And Culex Pipiens Complex (Diptera: Culicidae) For Dirofilaria Immitis (Spirurida: Onchocercidae) In Northern California, Jeffrey Allan Kurosaka

University of the Pacific Theses and Dissertations

Dirofilaria immitis Leidy (dog heartworm) is a life-threatening parasite transmitted by mosquitoes to domestic dogs. Endemic in the eastern United States, cases have become more prevalent over the last few decades. While prevalence in California is generally low, Lake and San Joaquin Counties have reported rates comparable to the East Coast at 3.73% and 0.71%(CAPC 2017), respectively. Aedes sierrensis is thought to be responsible for transmission in California, but in some cases, it exists in inadequate quantities and temporal ranges to explain parasite activity. Based on Huang et al. (2013) and Tran (2016), bloodfeeding patterns, and other vector criteria, Culex …

The Presumptions Of Classical Liberal Constitutionalism, Matthew J. Lindsay

All Faculty Scholarship

Richard A. Epstein’s The Classical Liberal Constitution is an imposing addition to the burgeoning body of legal scholarship that seeks to “restore” a robust conception of economic liberty and limited government to its rightful place at the center of American constitutionalism. Legislators and judges operating within a “classical liberal conception of government,” Epstein explains, would approach skeptically “[a]ll [regulatory] proposals that deviate from the basic common law protections of life, liberty, and property.” Classical liberal constitutional courts would thus renounce the toothless rational basis review of the post-New Deal “progressive mindset,” and instead subject to exacting scrutiny the government’s “purported …

The Impacts Of Wheat Gluten Products And Short-Chain Fructooligosaccharides On The Health And Production Of Juvenile Rainbow Trout (Oncorhynchus Mykiss), Samuel W. Voller

Other Faculty of Science and Engineering Theses

The impacts of wheat gluten products and short-chain fructooligosaccharides on the health and production of juvenile rainbow trout (Oncorhynchus mykiss) Samuel W. Voller Through the implementation of in vivo feeding trials, the efficacy of three wheat gluten (WG) products, vital (Amytex®), hydrolysed (Merripro®) and soluble hydrolysed (Solpro®) wheat gluten as replacement of soy protein concentrate, and scFOS prebiotic (Profeed®) supplementation were analysed to assess their impacts on intestinal health and production of juvenile rainbow trout. Microbial community analysis in experiment one revealed a degree of diet based modulation with 7.5% and 15% inclusions of wheat gluten (WG) products. Bacterial species …

Healthy Performance - A Case Study On Performance Management, Jeremy Walding

Other Faculty of Arts, Humanities and Business Theses

The following thesis has set out to identify the current gaps in knowledge, literature and research surrounding performance management within the English National Health Service (NHS). A literature review and qualitative case study were conducted on the factors associated with organisational and strategic performance management within the English NHS. The study sets out to close the gaps in knowledge surrounding performance management to identify an alternative approach towards delivery. Current literature indicates that performance management frameworks within the NHS that may have been influenced by the impact of New Public Management movement approaches are very much based on a ‘command …

The Use Of Employment/Vocational Rehabilitation Services For Persons With Hiv/Aids And Substance Abuse: A Potential Health Benefit, Abdoulaye Diallo, Chia Vang, Belinda Rivas, Antonio Aguirre, Carl Flowers

School of Rehabilitation Services & Counseling Faculty Publications

This study investigates Vocational Rehabilitation (VR) services related to competitive employment closure among non-Hispanic Blacks (NHB), non-Hispanic Whites (NHW), and Hispanics with HIV/AIDS and substance use disorder (SUD). Data of 4150 was extracted from the Rehabilitation Services Administration (RSA-911) database. Descriptive statistics and multi-variate analysis showed consumers who received SSI and Medicare were less likely to be employed than those who did not, and, NHW who received rehabilitation counseling and guidance benefited the most compared to NHB who did not. Rehabilitation counselors can assist minorities with HIV/AIDS and SUD secure employment given the positive effects of employment for this population. …

Surgical Plume And Its Implications: A Review Of The Risk And Barriers To A Safe Work Place, E. Tan, K. Russell

Nursing Papers and Journal Articles

Every year thousands of health care professionals worldwide are exposed to surgical smoke. There is evidence that this smoke consists of toxic gases, pathogens and particulate matter that is a hazard for patients and the perioperative team. Past research indicates that perioperative staff inconsistently comply with smoke evacuation recommendations. The aim of this study was to identify, review and discuss the issues related to surgical plume and its implications for patients and perioperative staff. The findings of this review relate to: surgical smoke content, its risks to the health of the perioperative staff, preventative measures, infection control measures, compliance with …

Using Tcr Transgenic, Gp100 Reactive T Cells And Checkpoint Inhibition To Target Lymphangioleimyomatosis, Christian Ankney

Master's Theses

Lymphangioleiomyomatosis (LAM) is a low-grade neoplastic disease affecting primarily women. It is characterized by cystic lung disease as well as renal and retroperitoneal tumors called angiomyolipomas and lymphangiomas. Tumor cells have smooth muscle features as well as neuroendocrine cell surface markers, and the disease can be diagnosed by HMB45 staining of tumor cells. We questioned whether expression of melanocytic antigens, specifically gp100, creates an opportunity to treat LAM by adoptive T cell transfer. LAM lung lesions demonstrate poor immune surveillance, therefore adoptive T cell transfer could offer benefits in this disease.

A link was made between melanoma and depigmenting vitiligo …

The Novel Characterization Of Extracellular Vesicles Containing Proteins Which Have Been Implicated In The Pathogenesis Of Hiv Associated Neurocognitive Disorders, Grace Ispas

Master's Theses

Although Anti-Retroviral Therapy (ART) is practiced, HIV-1 positive individuals still experience HIV-Associated Neurocognitive Disorders (HAND), collectively described as the presentation of neurocognitive, behavioral and motor dysfunctions that decrease the quality of life, while increasing the mortality in ART suppressed HIV-1 positive patients.

Current literature suggests that extracellular vesicles are involved in the pathogenesis of HAND as they are believed to be transferring HIV-1 proteins to nearby neuronal cells. Although most studies assume homogeneity among populations, characterizing cellular proteins or RNA levels in bulk, we hypothesize that distinct populations of extracellular vesicles are released and that environmental conditions, including viral infections, …

Optimizing Clean Catch Urine Collection And Its Applications In Urinary Microbiome Studies, Danielle Johansen

Master's Theses

Clean catch was developed before the advancement of more sensitive assays for culturing urinary bacteria. I reassessed clean catch, endeavoring to optimize this method. Periurethral swabs and voided urines were routinely collected, while analyzing different aspects of the method. I determined that midstream urine contained the least amount of vulvo-vaginal contamination and that the time of collection did not impact the results. I also determined that the use of antiseptic wipes prior to void increased the level of vulvo-vaginal contamination. A small cohort of females collected daily urines over a 17-day period and kept a strict alcohol diary. Females who …

Dexamethasone Treatment Effects On H3k27me3 Chromatin Organization Is Related To Nk Cell Immune Dysregulation, Michael Sarafeno Misale

Master's Theses

It is well-established that psychological stress reduces natural killer (NK) cell immune function. This reduction is mediated by stress-induced release of glucocorticoids (GC), which can suppress immune function. Associated with suppression of a particular immune function are GC induced histone-epigenetic marks. Histone-epigenetic marks are responsible for the organization and compartmentalization of genomes into transcriptionally active euchromatin domains that are localized to the interior of the nucleus. Transcriptionally silent heterochromatic domains are enriched with methylated epigenetic marks and are localized to the nuclear periphery. The purpose of this investigation was to assess the influence of GC on H3K27me3 chromatin organization by …

Multifunctional Regulatory T Cells From Human Umbilical Cord Blood And The Role Of Tumor Necrosis Factor In Immune Homeostasis, Alexander Nelson

Master's Theses

Regulatory T cells (Tregs) are required to suppress inflammation and prevent autoimmunity. During fetal development Tregs are crucial to maintain tolerance between mother and child. After birth, neonates require tolerance to avoid harmful immune responses to foreign antigens in food and allow colonization with commensal microbes. We demonstrate a propensity for T cells in human umbilical cord blood to differentiate into Tregs in response to antigen receptor stimulation ex vivo. Cord blood-derived Tregs potently suppress T cell proliferation, but also produce pro-inflammatory cytokines known to activate innate immune responses. These results suggest that antigen exposure during early life results in …

Characterization Of Inhibitory Molecules Produced By Lactobacillus Crispatus, Giuseppe Anthony Pistone

Master's Theses

Lactobacillus species are widely accepted as beneficial bacteria of the human microbiota1-8. Lactobacilli spp. are well documented to inhibit pathogens by production and secretion of hydrogen peroxide (H2O2), organic acids, and/or proteinaceous bacteriocins into their environment. Most research attributes bactericidal activity of cell free supernatant (CFS) to H2O2 and/or lactic acid. Here, I demonstrate that CFS from a clinical isolate of Lactobacillus crispatus contains a molecule(s) that inhibits uropathogenic Escherichia coli (UPEC) colony formation independently of H2O2 and organic acids. Physiologic concentrations of H2O2 and organic acids produced by L. crispatus do not inhibit UPEC colony formation. Incubation of UPEC …

High Resolution Melting: A Useful Field-Deployable Method To Measure Dhfr And Dhps Drug Resistance In Both Highly And Lowly Endemic Plasmodium Populations, Yaye Dié Ndiaye, Cyrille K. Diédhiou, Amy K. Bei, Baba Dieye, Aminata Mbaye, Nasserdine Papa Mze, Rachel F. Daniels, Ibrahima M. Ndiaye, Awa B. Déme, Amy Gaye, Mouhamad Sy, Tolla Ndiaye, Aida S. Badiane, Mouhamadou Ndiaye, Zul Premji, Dyann F. Wirth, Souleymane Mboup, Donald Krogstad, Sarah K. Volkman, Ambroise D. Ahouidi, Daouda Ndiaye

Pathology, East Africa

Background: Emergence and spread of drug resistance to every anti-malarial used to date, creates an urgent need for development of sensitive, specifc and feld-deployable molecular tools for detection and surveillance of validated drug resistance markers. Such tools would allow early detection of mutations in resistance loci. The aim of this study was to compare common population signatures and drug resistance marker frequencies between two populations with diferent levels of malaria endemicity and history of anti-malarial drug use: Tanzania and Sénégal. This was accomplished by implementing a high resolution melting assay to study molecular markers of drug resistance as compared to …

Reference Intervals For Thyroid Stimulating Hormone And Free Thyroxine Derived From Neonates Undergoing Routine Screening For Congenital Hypothyroidism At A University Teaching Hospital In Nairobi, Kenya: A Cross Sectional Study, Geoffrey Omuse, Ali Kassim, Francis Kiigu, Ra'ana Hussain, Mary Limbe

Pathology, East Africa

Background: In order to accurately interpret neonatal thyroid function tests (TFTs), it is necessary to have population specific reference intervals (RIs) as there is significant variation across different populations possibly due to genetic, environmental or analytical issues. Despite the importance of RIs, globally there are very few publications on RIs for neonatal TFTs primarily due to ethical and technical issues surrounding recruitment of neonates for a prospective study. To the best of our knowledge, this is the first report from Africa on neonatal RIs for TFTs.

Methods: We used hospital based data largely derived from neonates attending the wellness clinic …

Protective Effect Of Anti-Phosphatidylserine Antibody In A Guinea Pig Model Of Advanced Hemorrhagic Arenavirus Infection, John M. Thomas, Philip E. Thorpe

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Objective: Host derived markers on virally infected cells or virions may provide targets for the generation of antiviral agents. Recently, we identified phosphatidylserine (PS) as a host marker of virions and virally-infected cells.

Methods and Materials: Under normal physiological conditions, PS is maintained on the inner leaflet of the plasma membrane facing the cytosol. Following viral infection, activation or pre-apoptotic changes cause PS to become externalized. We have previously shown that bavituximab, a chimeric human-mouse antibody that binds PS complexed with β2-glycoprotein I (β2GP1), protected rodents against lethal Pichinde virus and cytomegalovirus infections.

Results: Here, we determined the antiviral activity …

Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep

Chulalongkorn University Theses and Dissertations (Chula ETD)

Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …

The Protective Effect Of Hydroxyxanthone On The Leakiness Of Intestinal Epithelia By The Proinflammatory Cytokine Related To Bacterial Endotoxin, Wannaporn Chayalak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Leaky gut characterized as decreased transepithelial electrical resistance and increased paracellular permeability to macromolecules is associated with several fatal diseases including sepsis. Pro-inflammatory cytokine IL-1β is a critical mediator of underlying mechanism through activation of myosin light chain kinase pathway. Blocking the inducible phosphorylated myosin light chain (p-MLC) indicates the successful treatment. Xanthones predominantly found in mangosteen (Garcinia mangostana) has been indicated as a potent anti-inflammatory action. Hydroxyxanthones (HDX), the major natural xanthones, have been recently synthesized in various forms. This study aimed to examine and screen the action of various forms of synthetic HDXs, in preventing the increased paracellular …

Mathematical Models For Treatment Time Of Pathogenic Infection In Blood After Antibiotics Administration, Panit Suavansri

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective in this thesis is to create a mathematical model to predict the period of time needed to clear out the pathogens from the bloodstream after the patient receives certain antibiotics. The result will lead to the time where the drug administration can be terminated. This research is to compute the densities of bacteria and malaria in bloodstream at any given time and to compare this level with the actual results of the patient's blood sampling, which was sent to the laboratory for investigation. The laboratory process of the blood sampling will usually take more than 2-3 days to …

Prevalence And Molacular Genetic Analysis Of Human Respiratory Syncytial Virus And Enterovirus 68 Among Children With Acute Lower Respiratory Tract Infection In Thailand, I-Lada Thongpan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Respiratory syncytial virus (RSV) causes acute lower respiratory tract infection in infants and young children worldwide. A clear description of local RSV molecular epidemiology, evolution, and transmission requires detailed sequence data and can inform new strategies for virus control and vaccine development. To investigate the RSV burden in Thailand over six consecutive years (January 2012 to December 2017). There were two different methods to detect RSV in the present study. First, we screened 3306 samples obtained from children ≤5 years old with acute respiratory tract infection using semi-nested reverse-transcription polymerase chain reaction (RT-PCR) during January 2012 to December 2015. Second, …

Antibacterial Agents From Dracaena Cochinchinensis (Lour.) S.C.Chen And Eleutherine Americana (Aubl.) Merr. Ex K. Heyne, Ritbey Ruga

Chulalongkorn University Theses and Dissertations (Chula ETD)

Fifteen compounds including two new anthraquinones (118-119), two naturally occurring new compounds (116-117) and eleven known compounds were successfully isolated from the CH2Cl2 extracts of Dracaena cochinchinensis and Eleutherine americana i.e. dihydrochalcones (5 and 23), pterostilbene (27), homoisoflavanones (15 and 82-84), naphthalenes (85 and 116), pyranonaphthoquinone (86) and anthraquinones (99-100 and 117-119). Among isolated compounds, (+)-eleutherin (86) was the most potent antibacterial agent against Propionibacterium acnes KCCM 41747, Staphylococcus aureus ATCC 25923, Streptococcus sobrinus KCCM 11898, Streptococcus mutans ATCC 25175 and Salmonella typhi ATCC 422 with inhibition zones of 14.7 to 18.0 mm and MIC values of 0.24 to 7.8 …

New Edta-Based Drug Delivery Carrier, Nathapong Pangkom

Chulalongkorn University Theses and Dissertations (Chula ETD)

Most of the anticancer drugs are insoluble in water, unspecified to target site and degradable under acidic conditions in the stomach, resulting in decreasing their activities and increasing the side effects. To overcome these problems, drug delivery carriers were used to overcome these problems. Most of the drug delivery carriers were prepared by polymeric micelle systems. However, that of polymeric micelle systems are difficult to synthesize into the random polymers, where different units are aligned on a polymer chain in a random manner, immature drug-incorporation technology and possible chronic liver toxicity due to slow metabolic process. Therefore, the enhancement of …