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Winter 2017 Gsbms Alumni Connections, New York Medical College

Graduate School of Biomedical Sciences

• The Latest Issue of Chironian Magazine is Now Online
• Department of Microbiology and Immunology Hosts Research Forum
• STAR Alumnus Keeps Up the Good Work

Hplc-Uv Quantitation Of Folate Synthesized By Rickettsia Endosymbiot Ixodes Pacificus (Reip), Junyan Chen

Cal Poly Humboldt theses and projects

Ticks are the most important vector of many infectious diseases in the United States. Understanding the nature of the relationship between Rickettsia endosymbiont Ixodes pacificus (REIP) and Exudes pacificus will help develop strategies for the control of tick-borne diseases, such as Lyme disease, and Rocky Mountain spotted fever. Folate, also known as vitamin B9, is a necessary vitamin for tick survival, and plays a central role in one-carbon metabolism in cells. Folate exist as a large family of structurally related forms that transfer one-carbon groups among biomolecules that are important to cell growth, differentiation, and survival. In Dr. Zheng’s lab, …

Do Antibiotics In Early Life Contribute To Obesity?, Shimon Kadosh

The Science Journal of the Lander College of Arts and Sciences

In recent years, science has made great strides in its understanding of the microbiome, discovering that it plays a role in regulating many body processes. One area of study is the microbiomes interaction and influence on host metabolic processes. Studies using both mice and humans have established a clear correlation between obesity and the composition of the microbiome, identifying a microbiome unique to obese individuals. Furthermore, experiments with germ-free mice have shown that the microbiome effects host metabolism, causing germ free mice to increase in mass when inoculated with normal microbiota. Inoculation with microbiota from obese mice yielded greater increases …

Termite Gut Microbes As Tools And Targets For Termite Control, Chinmay Vijay Tikhe

LSU Doctoral Dissertations

The Formosan subterranean termite (FST), Coptotermes formosanus, is an invasive urban pest in the United States. Colonies of the FST are dependent on the symbiotic gut protozoa for cellulose digestion in the workers’ guts, and the gut bacterial community is known to provide essential nutrients to the termite. The objectives of this PhD research were to develop and evaluate paratransgenesis and phage therapy for termite control. During this study, a termite gut bacterium: Trabulsiella odontotermitis was genetically engineered and was evaluated as a ‘Trojan horse’ for paratransgenesis. We proved that T. odontotermitis can tolerate 50 times more concentration of ligand-Hecate …

Quantitation Of Anti-Pythium Insidiosum Antibodies Before And After Immunotherapy In Healthy Dogs, Carmen Beatriz Arsuaga

LSU Master's Theses

Pythium insidiosum is an aquatic oomycete that causes invasive, progressive granulomatous lesions of the skin in dogs, horses, and cats, and of the gastrointestinal tract in dogs. Quantitation of anti-P. insidiosum IgG antibodies can be used in dogs to both confirm a suspected diagnosis and to monitor response to therapy. Recently, an immunotherapeutic product (IP) has been marketed for the treatment of pythiosis in dogs, horses, and people. The aim of this study was to evaluate the effect of administration of this product on anti-P. insidiosum IgG concentrations in dogs. The IP was administered to seven, healthy hound mixes on …

On-Site Sperm Cryopreservation For Aquatic Repository Development And User Group Linkage, William Martin Childress

LSU Master's Theses

Although on-site cryopreservation of aquatic species sperm in nitrogen-vapor shipping dewars has been used for more than 30 yr, many limitations remain. Due to the size of shipping dewars, most studies are small scale and can only produce tens of samples. The freezing temperatures that can be achieved are also affected by the size of the dewar and the number of samples being frozen due to the heat load. In addition, often due to timing or remote location these samples cannot be shipped, and if they are shipped, quality can be lost and the samples must be discarded. The goal …

Louisiana Birds Act As Reservoirs For Antibiotic-Resistant Bacteria, Collin T. Brown

LSU Master's Theses

Wild birds carry diverse microbial communities, including antibiotic-resistant bacteria (ARB). With the ever-increasing use of antibiotics in agricultural and clinical settings, genes that code for antibiotic resistance in bacteria have been selected for. These bacteria persist in the environment in a culturable state, but little is understood about communities of antibiotic-resistant bacteria in the environment. Birds with predictable behaviors may serve as useful indicators of these communities, and provide insights into how bacterial communities spread and evolve in the environment. To understand the utility of birds as indicators of the presence of antibiotic-resistant bacteria in the environment, we collected bacterial …

Building A Paradise? On The Quest For The Optimal Human Habitat, Anu Besson

Contemporary Aesthetics (Journal Archive)

Do humans have a natural habitat? If yes, is it the original habitat of early hominids or the most optimal environment for today’s humans? Are these two the same thing and, if not, what does ‘optimal habitat’ mean? I examine the concept of the optimal habitat from four viewpoints: 1) paradise; 2) urban design based on environmental psychology; 3) favorite places; and 4) environment as an invitation for action. I conclude that an optimal habitat is not a collection of more or less fixed elements but an environment that can be experienced as a beneficial feedback loop based on and …

Readiness For Antimicrobial Resistance (Amr) Surveillance In Pakistan; A Model For Laboratory Strengthening, Dania Khalid Saeed, Rumina Hasan, Mahwish Naim, Afia Zafar, Erum Khan, Kauser Jabeen, Seema Irfan, Imran Ahmed Haq, Mohammad Zeeshan, Zabin Wajidali, Joveria Farooqi, Sadia Shakoor, Abdul Chagla, Jason Roy

Department of Pathology and Laboratory Medicine

Background: Limited capacity of laboratories for antimicrobial susceptibility testing (AST) presents a critical diagnostic bottleneck in resource limited countries. This paper aims to identify such gaps and to explore whether laboratory networks could contribute towards improving AST in low resource settings.
Methods: A self-assessment tool to assess antimicrobial susceptibility testing capacity was administered as a pre-workshop activity to participants from 30 microbiology laboratories in 3 cities in Pakistan. Data from public and private laboratories was analyzed and capacity of each scored in percentage terms. Laboratories from Karachi were invited to join a support network. A cohort of five laboratories that …

Mucosal Fluid Glycoprotein Dmbt1 Suppresses Twitching Motility And Virulence Of The Opportunistic Pathogen Pseudomonas Aeruginosa, Jianfang Li, Matteo E. O. Metruccio, David J. Evans, Suzanne M. J. Fleiszig

Faculty Publications & Research of the TUC College of Pharmacy

It is generally thought that mucosal fluids protect underlying epithelial surfaces against opportunistic infection via their antimicrobial activity. However, our published data show that human tear fluid can protect against the major opportunistic pathogen Pseudomonas aeruginosa independently of bacteriostatic activity. Here, we explored the mechanisms for tear protection, focusing on impacts of tear fluid on bacterial virulence factor expression. Results showed that tear fluid suppressed twitching motility, a type of surface-associated movement conferred by pili. Previously, we showed that twitching is critical for P. aeruginosa traversal of corneal epithelia, exit from epithelial cells after internalization, and corneal virulence. Inhibition …

A Rare Case Of Erythema Elevatum Diutinum Presenting As Diffuse Neuropathy, G H. Nguyen, E L. Guo, D Norris

Faculty, Staff and Students Publications

No abstract provided.

Multigenic Dna Vaccine Induces Protective Cross-Reactive T Cell Responses Against Heterologous Influenza Virus In Nonhuman Primates, Merika T. Koday, Jolie A. Leonard, Paul Munson, Adriana Forero, Michael Koday, Debra L. Bratt, Karen Duus

College of Osteopathic Medicine (TUN) Publications and Research

Recent avian and swine-origin influenza virus outbreaks illustrate the ongoing threat of influenza pandemics. We investigated immunogenicity and protective efficacy of a multi-antigen (MA) universal influenza DNA vaccine consisting of HA, M2, and NP antigens in cynomolgus macaques. Following challenge with a heterologous pandemic H1N1 strain, vaccinated animals exhibited significantly lower viral loads and more rapid viral clearance when compared to unvaccinated controls. The MA DNA vaccine induced robust serum and mucosal antibody responses but these high antibody titers were not broadly neutralizing. In contrast, the vaccine induced broadly-reactive NP specific T cell responses that cross-reacted with the challenge virus …

Fibroblast Growth Factor 21 Is A Novel Protein Sensor In Pregnancy, Elizabeth Frost Sutton

LSU Doctoral Dissertations

The twenty-first century has experienced a shift in cause of death worldwide from communicable diseases to noncommunicable diseases. Interestingly, many of these implicated chronic diseases, such as cancer, diabetes, and cardiovascular disease, have been shown to be programmed in the womb. As first posited by the Barker Hypothesis, adverse exposures in utero can increase an individual’s risk for chronic disease later in life. Therefore, pregnancy is an opportune time for intervention to improve the health of future generations. Studies of exposures known to negatively impact infant health, e.g. states of overnutrition (obesity, diabetes, excess gestational weight gain) and undernutrition (starvation, …

Anaemia Of Chronic Kidney Disease: What We Know Now, Anoushka R. Krishnan, Debbie Trinder, Anita C. G. Chua, Aron Chakera, Grant A. Ramm, John K. Olynyk

Research outputs 2014 to 2021

Our understanding of the pathophysiology of the anaemia of chronic kidney disease (CKD) has improved considerably in the last decade with the discovery of the iron regulatory peptide hepcidin. Reduced clearance of hepcidin and the presence of a chronic inflammatory state contribute to elevated hepcidin levels in kidney disease. The recent discovery of the various factors and signalling pathways regulating hepcidin has opened up an exciting avenue for research into the development of newer agents that could treat anaemia of CKD. This review highlights our current understanding of iron metabolism in health, the regulators of hepcidin, issues associated with the …

Longer-Term Impact Of Highand Low Temperature On Mortality: An International Study To Clarify Length Of Mortality Displacement, Ben Armstrong, Michelle Bell, Micheline De Sousa Zanotti Stagliorio Coelho, Yue Liang Leon Guo, Yuming Guo, Patrick J. Goodman, Masahiro Hashizume, Yasushi Honda, Ho Kim, Eric Lavigne, Paola Michelozzi, Paulo Hilario Nascimento Saldiva, Joel Schwartz, Matteo Scortichini, Francesco Sera, Aurelio Tobias, Shilu Tong, Chang Fu Wu, Antonella Zanobetti, Ariana Zeka, Antonio Gasparrini

Articles

In many places, daily mortality has been shown to increase after days with particularly high or low temperatures, but such daily time-series studies cannot identify whether such increases reflect substantial life shortening or short-term displacement of deaths (harvesting).

Pcr-Ribotype Distribution Of Clostridium Difficile In Irish Pigs, Katharina Stein, Sarah Egan, Helen Lynch, Celine Harmanus, Lorraine Kyne, Celine Herra, Sinead Mcdermott, Ed Kuijper, Fidelma Fitzpatrick, Susan Fitzgerald, Lynda Fenelon, Denise Drudy

Articles

Clostridium difficile is an important enteric pathogen in humans causing infections in the healthcare environment and the community. Carriage of C. difficile and C. difficile-related enterocolitis has been reported in piglets worldwide. The aim of this study was to investigate the rates of C. difficile isolation from pigs in Ireland. Faecal samples from piglet litters and sows were collected from six farms in 2015. The sows were non-diarrhoeal at the time of sampling. The diarrhoeal status of the piglets was unknown. C. difficile was isolated from 34/44 (77%) of piglet litter samples and from 33/156 (21%) of sow samples. The …

Habitat Usage Of Tri-Colored Bats (Perimyotis Subflavus) In Western Kentucky And Tennessee Post-White Nose Syndrome, Katherine Schaefer

Murray State Theses and Dissertations

The tri-colored bat (Perimyotis subflavus) has declined severely across its range since 2006 due to white nose syndrome, a fungal disease causing massive bat mortality in North America. My objective was to determine distinguishing characteristics of roost trees and habitat used by tri-colored bats so that the species’ needs can be considered in management plans. I mist-netted for tri-colored bats in western Kentucky and Tennessee during the summers of 2015 and 2016, and attached a radio transmitter to each captured adult bat. I tracked 15 bats to their day roosts and collected habitat data at 38 roost areas …

A Presence-Only Species Distribution Model Comparison Predicting The Distribution Of The Amphibian Chytrid Fungus (Batrachochytrium Dendrobatidis), Melanie L. Torres

Murray State Theses and Dissertations

Global anthropogenic impacts have incited devastating effects on both human and wildlife populations. Emerging infectious diseases (EID) are one consequence of these impacts. One particular EID, chytridiomycosis, is a threat to global amphibian populations, causing massive die-offs and several species extinctions. While predictive modeling has helped the general understanding of where the aquatic pathogen causing chytridiomycosis, Batrachochytrium dendrobatidis (Bd), could spread to, most models are on continental or countrywide scales. This coarse-scale modeling makes local management and conservation planning for imperiled amphibians difficult. In addition, modeling efforts can vary depending on location and species, making it necessary to test the …