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Bacteriophages Phi Mr299-2 And Phi Nh-4 Can Eliminate Pseudomonas Aeruginosa In The Murine Lung And On Cystic Fibrosis Lung Airway Cells, Aidan Coffey, Et. Al.

Department of Biological Sciences Publications

Pseudomonas aeruginosa is a common cause of infection in the lungs of patients with cystic fibrosis (CF). In addition, biofilm formation and antibiotic resistance of Pseudomonas are major problems that can complicate antibiotic therapy. We evaluated the efficacy of using bacteriophages to kill the pathogen in both biofilms and in the murine lung. We isolated and characterized two phages from a local wastewater treatment plant, a myovirus (ϕNH-4) and a podovirus (ϕMR299-2). Both phages were active against clinical isolates of P. aeruginosa. Together, the two phages killed all 9 clinical isolate strains tested, including both mucoid and nonmucoid strains. An …

Learning Without Being Taught: A Look At How Schools, The Home And The Neighborhood Influence "Race" Conceptualization, Owen Christopher Gaither

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Where do we get our ideas about the concept of `race'? The conceptualization of `race' has long been a topic of interest in the social sciences and society in general. The word `race' has been used and defined in different ways and different purposes throughout U.S. history. The definition of `race' therefore is arbitrary, changing according to the situation, but the consequences of how the word `race' is used are concrete and effect peoples lives daily. This research, in accord with much of the literature on the topic, shows that public schools play a major role in the conceptualization …

Investigation Into The Mechanism Of Salicylate-Associated Genotypic Antibiotic Resistance In Staphylococcus Aureus, Nada Salah Helal

USF Tampa Graduate Theses and Dissertations

Growth of Staphylococcus aureus with the NSAID salicylate increases phenotypic resistance (SAPAR), and the frequency at which heritable resistance occurs to various antibiotics (SAGAR). This study describes the effect of salicylate on heritable and phenotypic resistance to a set of antibiotics for laboratory and multi-drug resistant strains of S. aureus and investigates the link between resistance and SAGAR. Drug gradient plates were used to determine phenotypic resistance to antibiotics targeting DNA replication, transcription, translation and the cell wall in the presence or absence of salicylate. To measure heritable resistance, mutation frequencies were determined for each antibiotic in the presence and …

Towards A Better Specification Of The Store Environment Stimulus: An Augmented Stimulus-Organism-Response (Sor) Model That Captures Brand Expressiveness, John Murray

Conference proceedings

Abstract: This paper proposes an augmented SOR model which facilitates design/architect practitioners when they review store concepts. The paper contributes to the knowledge base of designers/architects when making deliberate brand expressions in development of the store environment. The global nature of the SOR model, it is argued, does not allow for discrimination between consumer interpretations of store brands; nor does it propose a realistic means of engaging design-architect practitioners at the concept proofing stage of development. This conceptual paper argues that retail branding studies benefit from inclusion of more flexible frameworks founded on separable and integral design-architecture and brand communicative …

Engraved In The Mind: The Significance Of Smell, Jane Levi

Dublin Gastronomy Symposium

No abstract provided.

Some Avenues For Feeling, Adam Frank

Criticism

The Particulars of Rapture: An Aesthetics of the Affects (Charles Altieri)
Compassion: The Culture and Politics of an Emotion (ed. Lauren Berlant)
Disgust: The Theory and History of a Strong Sensation (Winfried Menninghaus)
Touching Feeling: Affect, Pedagogy, Performativity (Eve Kosofsky Sedgwick)

Introduction: What Is Reenactment?, Vanessa Agnew

Criticism

No abstract provided.

Introduction: Materia Media, Cannon Schmitt

Criticism

No abstract provided.

An Investigation Of Nanos1 Function During Primordial Germ Cell Development In Xenopus Laevis., Fangfang Lai

Open Access Dissertations

Nanos is expressed in multipotent cells, stem cells, and primordial germ cells (PGCs) of organisms as diverse as jellyfish and humans. It functions together with Pumilio to translationally repress targeted mRNAs. Here we show by loss-of-function experiments that Xenopus Nanos1 is required to preserve PGC fate. Morpholino knockdown of maternal Nanos1 resulted in a striking decrease in PGCs and loss of germ cells from the gonads. Lineage tracing and TUNEL staining reveals that Nanos1 deficient PGCs fail to migrate out of the endoderm. They appear to undergo apoptosis rather than convert to normal endoderm. Whereas normal PGCs do not become …

The Pseudomonas Syringae Hrpj Protein Controls The Secretion Of Type Iii Translocator Proteins And Has A Virulence Role Inside Plant Cells, Emerson Crabill, Andrew Karpisek, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

The bacterial plant pathogen Pseudomonas syringae injects effector proteins into plant cells via a type III secretion system (T3SS), which is required for pathogenesis. The protein HrpJ is secreted by P. syringae and is required for a fully functional T3SS. A hrpJ mutant is non-pathogenic and cannot inject effectors into plant cells or secrete the harpin HrpZ1. Here we show that the hrpJ mutant also cannot secrete the harpins HrpW1 and HopAK1 or the translocator HrpK1, suggesting that these proteins are required in the translocation (injection) of effectors into plant cells. Complementation of the hrpJ mutant with secretion incompetent HrpJ …

06/05/2012 Obituary Records, Hawaii Obituaries

Obituaries

List of Obituaries for individuals who passed away on 06/05/2012. Scroll down to see the names and obituary description.

Progressive Entanglements Religious Intimacy In Japanese–Filipino Marriages, Mario Ivan Lopez

Philippine Studies: Historical and Ethnographic Viewpoints

Studies on international marriages often focus on how gender, sexuality, race, and ethnicity are constructed and articulated through the migration process. The influence of religion as one articulator in migrants’ lives and its influence on the family tend to be ignored, but arguably individuals’ faith must be included in the research agenda. Using ethnographic examples, thisarticle traces the religious practices that develop in the lives of some Japanese–Filipino couples. Thisarticle highlights not just the transnational mobility of migrants, but also the movement of their faith and its potential impact on intimate relations within migrant homes and communities postmigration.

Blog Post, T-Minus 5 Days And Counting…, 'Burg Blogs, June 5, 2012, Stephanie Lawler

USF St. Petersburg Campus News and Press Releases

No abstract provided.

High-Activity Mutants Of Butyrylcholinesterase For Cocaine Hydrolysis And Method Of Generating The Same, Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai

Pharmaceutical Sciences Faculty Patents

A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, …

Human Impact On Atolls Leads To Coral Loss And Community Homogenisation: A Modeling Study, Bernhard Riegl, Charles R. C. Sheppard, Samuel J. Purkis

Marine & Environmental Sciences Faculty Articles

We explore impacts on pristine atolls subjected to anthropogenic near-field (human habitation) and far-field (climate and environmental change) pressure. Using literature data of human impacts on reefs, we parameterize forecast models to evaluate trajectories in coral cover under impact scenarios that primarily act via recruitment and increased mortality of larger corals. From surveys across the Chagos, we investigate the regeneration dynamics of coral populations distant from human habitation after natural disturbances. Using a size-based mathematical model based on a time-series of coral community and population data from 1999–2006, we provide hind- and forecast data for coral population dynamics within lagoons …

Methods For Increasing Potency Of Adult Mesenchymal Stem Cells, Kiminobu Sugaya, Angel Alvarez

UCF Patents

Disclosed herein are methods and materials for producing a more developmentally potent cell from a less developmentally potent cell. Specifically exemplified herein are methods that comprise introducing an expressible dedifferentiating polynucleotide sequence into a less developmentally potent cell, wherein the transfected less developmentally potent cell becomes a more developmentally potent cell capable of differentiating to a less developmentally potent cell of its lineage of origin or a different lineage.

Mammalian Multipotent Stem Cells And Compositions, Methods Of Preparation And Methods Of Administration Thereof, Kiminobu Sugaya, Christopher Brannen, Xiajing Dong, Hojoong Kim, Jose Pulido, Tingyu Qu, Ankur Vaghani

UCF Patents

This invention provides methods for preparing novel mammalian multipotent stem cells (MSCs), compositions thereof, and methods of preparing and administering the cells.

Minimal Cut Sets As Computational Tool In Metabolic Engineering, Steffen Klamt

Metabolic Engineering IX

No abstract provided.

Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan Greenhagen

Plant and Soil Sciences Faculty Patents

The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.

Effect Of Low Temperature Thermal Treatment On Soils Contaminated With Pentachlorophenol And Environmentally Persistent Free Radicals, Albert Leo N.Dela Cruz, Robert L. Cook, Slawomir M. Lomnicki, Barry Dellinger

Faculty Publications

The effect of low temperature thermal treatment on soils from a former Superfund wood-treating site contaminated with pentachlorophenol (PCP) and the environmentally persistent free radical (EPFR), pentachlorophenoxyl, was determined. The pentachlorophenoxyl EPFRs' and the PCP molecules' chemical behavior were simultaneously monitored at temperatures ranging from 25 to 300 °C via electron paramagnetic resonance (EPR) spectroscopy and GC-MS analysis, respectively. Two types of thermal treatment were employed: a closed heating (oxygen-starved condition) where the soil was heated under vacuum and an open heating system (oxygen-rich conditions), where the soil was heated in ambient air. EPR analyses for closed heating indicated the …

Human Genome-Wide Association And Mouse Knockout Approaches Identify Platelet Supervillin As An Inhibitor Of Thrombus Formation Under Shear Stress., Leonard C. Edelstein, Elizabeth J. Luna, Ian B. Gibson, Molly Bray, Ying Jin, Altaf Kondkar, Srikanth Nagalla, Nacima Hadjout-Rabi, Tara C. Smith, Daniel Covarrubias, Stephen N. Jones, Firdos Ahmad, Moritz Stolla, Xianguo Kong, Zhiyou Fang, Wolfgang Bergmeier, Chad Shaw, Suzanne M. Leal, Paul Bray

Department of Medicine Faculty Papers

BACKGROUND: High shear force critically regulates platelet adhesion and thrombus formation during ischemic vascular events. To identify genetic factors that influence platelet thrombus formation under high shear stress, we performed a genome-wide association study and confirmatory experiments in human and animal platelets.

METHODS AND RESULTS: Closure times in the shear-dependent platelet function analyzer (PFA)-100 were measured on healthy, nondiabetic European Americans (n=125) and blacks (n=116). A genome-wide association (P

CONCLUSIONS: We show for the first time that (1) platelets contain supervillin; (2) platelet thrombus formation in the PFA-100 is associated with human SVIL variants and low SVIL expression; and (3) …

(−)-Epigallocatechin Gallate Derivatives For Inhibiting Proteasome, Tak-Hang Chan, Wai-Har Lam, Larry Ming-Cheung Chow, Qing Ping Dou, Deborah Joyce Kuhn, Aslamuzzaman Kazi, Sheng Biao Wan, Kristin R. Landis-Piwowar

USF Patents

(−)-EGCG, the most abundant catechin, was found to be chemopreventive and anticancer agent. However, (−)-EGCG has at least one limitation: it gives poor bioavailability. This invention provides compounds of generally formulae below, wherein R11, R12, R13, R21, R22, R2, R3, and R4 are each independently selected from the group consisting of —H, and C1 to C10 acyloxyl group; and R5 is selected from the group consisting of —H, C1-C10-alkyl, C2-C10-alkenyl, C2-C10-alkynyl, C3-C7-cycloalkyl, phenyl, benzyl and C3-C7-cycloalkenyl, whereas each of the last mentioned 7 groups can be substituted with any combination of one to six halogen atoms; at least one of …

Microwave-Assisted Formation Of Sulfonium Photoacid Generators, Kevin Belfield, Ciceron Yanez

UCF Patents

We developed a fast, efficient method to prepare commercially important sulfonium salt photoacid generators (PAGs) using microwave-assisted reactions.

Orientation And Characteristics Of Subject Service In Basic Natural Science Based On The Investigation In Wuhan University, China, Hu Yongsheng

Proceedings of the IATUL Conferences

After investigating service requirements in basic natural science disciplines in Wuhan University, China, we analyze the characteristics according to the survey results, then provide the service strategies suitable for the science subject service. Five colleges in Wuhan University, including physics, chemistry, biology, mathematics, and environment, were investigated. The questionnaires include the frequency of science users using the library or the department reference room, how to get the resources for research, main document types used in the library or the department reference room, satisfaction rate in subject resources, training demand of science users, choice of service pattern, user’s expectation of subject …

Med5(Nut1) And Med17(Srb4) Are Direct Targets Of Mediator Histone H4 Tail Interactions, Zhongle Liu, Lawrence C. Myers

Dartmouth Scholarship

The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. In addition to its canonical role in transcriptional activation, recent studies have demonstrated that S. cerevisiae Mediator can interact directly with nucleosomes, and their histone tails. Mutations in Mediator subunits have shown that Mediator and certain chromatin structures mutually impact each other structurally and functionally in vivo. We have taken a UV photo cross-linking approach to further delineate the molecular basis of Mediator chromatin interactions and help determine whether the impact of certain Mediator mutants on chromatin is direct. Specifically, by using histone …

New Rfid-Apps: Hf-Based Stock Management By Mobile Solution And Producing Usage Statistics On Non-Lending Colleges (E.G. Periodicals), Hardy Zissel, Jan Kissig, Frank Seeliger

Proceedings of the IATUL Conferences

With this contribution we will provide an outline on new applications of RFID-utilization which go beyond the typical applications such as self-check service to clients and security tasks and may give a perspective of new ideas.

The German market of RFID-related tenders in the library context has an annual value of estimated at ten million Euro on average. It is our belief that innovative ideas and insights from within the library world itself are needed to advance the technological support and improve our goals, services and workflows in the small and smart world of the internet of things.

Against the …

A Genetic Study Of Ssv1, The Prototypical Fusellovirus, Eric Iverson, Kenneth M. Stedman

Biology Faculty Publications and Presentations

Viruses of thermophilic Archaea are unique in both their structures and genomic sequences. The most widespread and arguably best studied are the lemon-shaped fuselloviruses. The spindle-shaped virus morphology is unique to Archaea but widespread therein. The best studied fusellovirus is SSV1 from Beppu, Japan, which infects Sulfolobus solfataricus. Very little is known about the function of the genes in the SSV1 genome. Recently we have developed genetic tools to analyze these genes. In this study, we have deleted three SSV1 open reading frames (ORFs) ranging from completely conserved to poorly conserved: VP2, d244, and b129. Deletion of the universally conserved …

Toward A Working Theory Of Neurorhetorics, Jeffrey L. Honnold

USF Tampa Graduate Theses and Dissertations

This piece makes the claim that rhetoric is first philosophy--before philosophy, epistemology, ontology, or any other field--or that rhetoric is, at the least, on equal footing as these fields because:

empathy--and thusly the impulse for communication--is physiologically hardwired into humans; special distinctions between human and animal are largely artificial constructions, as is evidenced by neurosciences; "hard" science, in the form of neurosciences, is providing entrance points & opportunities for rhetoric to raise its status within the academy; and said neurosciences, in addition to empathy studies, have shown strong evidence supporting linguistic and evolutionary links between humans and other species, thereby …

Northern Ireland Food Culture: Moving Beyond Spuds, Aoibeann Walsh, Roy Nelson

Dublin Gastronomy Symposium

No abstract provided.

Transcendental Thermodynamics, Richard E. Morel, George Fleck

Kahn Institute Projects

Thermodynamics is often viewed as a narrow, introspective discipline, trapped by its origins in the 18th and 19th centuries. By dramatic contrast, we show that the Fourth Law of Thermodynamics provides explanations and interpretations of all natural events, extending across artificial boundaries of tradition- al academic disciplines. The Fourth Law of Thermodynamics states that far-from-equilibrium systems increase entropy at the maximum rate available to them. This broadly inclusive paradigm applies to systems from molecules, to organisms, to the biosphere. The Fourth Law is the Law of Evolution. All systems that communicate with their environment exhibit self-organization and self-optimization, enabling the …