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2011

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Climate Services To Support Sustainable Tourism And Adaptation To Climate Change, D.J. Scott, Christopher J. Lemieux, Leslie Malone Jan 2011

Climate Services To Support Sustainable Tourism And Adaptation To Climate Change, D.J. Scott, Christopher J. Lemieux, Leslie Malone

Geography and Environmental Studies Faculty Publications

Tourism is one of the largest global economic sectors, is a vital contributor to the economy of many nations, and is highly promoted as an important means of future development and poverty reduction in developing countries. The interface between climate and tourism is multifaceted and complex, with broad significance for tourist decision-making and expenditures, as well as industry marketing and operations worldwide. With the close relationship of tourism to the environment and climate, the integrated effects of climate change are anticipated to markedly affect tourism businesses and destinations, as well as the destination choices and mobility of individual tourists in …

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How Mri Can Contribute To The Diagnosis Of Acute Demyelinating Encephalomyelitis In Children, Emna Daoud, Imen Chabchoub, Henda Neji, Nozha Toumi, Rim B. Abdallah, Hajer Aloulou, Fatma Kammoun, Abdelmajid Mahfoudh, Mongia Hachicha, Jamel Mnif, Kheireddine B. Mahfoudh, Zeineb Mnif Jan 2011

How Mri Can Contribute To The Diagnosis Of Acute Demyelinating Encephalomyelitis In Children, Emna Daoud, Imen Chabchoub, Henda Neji, Nozha Toumi, Rim B. Abdallah, Hajer Aloulou, Fatma Kammoun, Abdelmajid Mahfoudh, Mongia Hachicha, Jamel Mnif, Kheireddine B. Mahfoudh, Zeineb Mnif

Neurosciences

OBJECTIVE: To illustrate through 10 pediatric cases, the clinical features, course, and importance of neuroimaging (especially MRI) in guiding the diagnosis of acute disseminated encephalomyelitis (ADEM) and controlling patients after treatment. METHODS: A retrospective review of 10 pediatric cases of ADEM, with special regard to the MRI features, presenting to the Pediatric Departments, Hedi Chaker Hospital, Sfax, Tunisia between January 2002 and December 2008. RESULTS: Children with ADEM presented with variable and multiple neurological signs most often occurring after an infectious episode, especially after upper respiratory tract infection. The MRI permitted confirmation of the diagnosis by showing demyelinating lesions either …

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Evaluation Of Viral Antibodies In Iranian Multiple Sclerosis Patients, Mohsen Khaki, Ali Ghazavi, Keyvan Ghasami, Mohammad Rafiei, Mohammah-Ali Payani, Ehsanollah Ghaznavi-Rad, Ghasem Mosayebi Jan 2011

Evaluation Of Viral Antibodies In Iranian Multiple Sclerosis Patients, Mohsen Khaki, Ali Ghazavi, Keyvan Ghasami, Mohammad Rafiei, Mohammah-Ali Payani, Ehsanollah Ghaznavi-Rad, Ghasem Mosayebi

Neurosciences

OBJECTIVE: To evaluate the viral antibodies in new Iranian multiple sclerosis (MS) patients. METHODS: In a cross-sectional study, sera from 61 MS patients and 60 healthy individuals were collected from January 2009 to March 2010 in the Immunology Department of Arak University of Medical Sciences, Arak, Iran, and examined for the presence of the anti-Epstein-Barr virus (EBV), human herpes virus 6 (HHV-6), measles, mumps, and para-influenza viruses IgG and IgM using an enzyme-linked immunosorbent assay or immunofluorescence. RESULTS: There were significant differences between the MS patients and the healthy individuals (controls) in the seroprevalence of anti-HHV-6 IgM (odds ratio [OR]=4.3, …

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The Characterization Of Pg0228 In Porphyromonas Gingivalis W83, Courtney Schlenker Jan 2011

The Characterization Of Pg0228 In Porphyromonas Gingivalis W83, Courtney Schlenker

Theses and Dissertations

Periodontitis affects 10 to 15 percent of most adult populations and can contribute to numerous systemic diseases. Porphyromonas gingivalis, a gram-negative anaerobic bacterium, is a recognized prime causative agent in periodontitis. Studies have shown a number of small non-coding RNAs (sRNAs) have been related to bacterial virulence. Many of these sRNAs require the facilitation of the bacterial Sm-like protein, Hfq, for optimum function. Hfq is a RNA chaperone involved in RNA stability, sRNA function, and polyadenylation. Mutants lacking in Hfq often show pleiotropic phenotypes, although the extent and severity of hfq null phenotypes is often species-specific. Hfq has been encoded …

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Acquired Cytogenetic Changes In Adult Twins Discordant For A History Of Childhood Sexual Abuse, Jenni Brumelle Jan 2011

Acquired Cytogenetic Changes In Adult Twins Discordant For A History Of Childhood Sexual Abuse, Jenni Brumelle

Theses and Dissertations

The primary study aim was to evaluate the latent biological effect of childhood sexual abuse (CSA) on adults by quantifying acquired cytogenetic changes and cortisol levels in identical twins who were discordant (N=22) or concordant (N=2) for a history of CSA. Although the difference scores for cortisol values between discordant identical co-twins were not significantly different from zero, a trend was observed for the twins exposed to intercourse, the most severe form of CSA, to have a blunted cortisol awakening response. Acquired cytogenetic changes were assessed by scoring telomere lengths and somatic cell abnormality frequencies via a cytokinesis-block micronucleus (MN) …

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Synthesis, Screening And Cocrystallization Of Adenosine Based Inhibitors With Methyltransferases, Ermc' And Ksga, Matthew Baker Jan 2011

Synthesis, Screening And Cocrystallization Of Adenosine Based Inhibitors With Methyltransferases, Ermc' And Ksga, Matthew Baker

Theses and Dissertations

Antibiotic resistance threatens to throw mankind back into an era when infectious disease was the predominant cause of death. In an effort to mitigate this danger, we targeted ErmC’ and KsgA. Both methylate N6-adenosine of ribosomal RNA, though each serve different roles in their bacterial host. ErmC’ dimethylates A2058 on 23S rRNA, conferring resistance to MLSB antibiotics (macrolides, lincosamides, streptogramin B). KsgA aids in ribosome assembly, binding inactive 30S until dimethylating A1518/A1519 of 16S rRNA. Like most methyltransferases, ErmC’ and KsgA use cofactor S-adenosylmethionine (SAM) as their methyl source, which binds adjacent to their specific adenosine substrate. ErmC’ inhibitors could …

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Analogous Mechanisms Of Resistance To Benzothiazinones And Dinitrobenzamides In Mycobacterium Smegmatis, Ana Luisa Ribeiro, Giulia Degiacomi, Fanny Ewann, Silvia Buroni, Maria Loreto Incandela, Laurent R. Chiarelli, Giorgia Mori, Jaeseung Kim, Monica Contreras-Dominguez, Young-Sam Park, Sung-Jun Han, Priscille Brodin, Giovanna Valentini, Menico Rizzi, Giovanna Riccardi, Maria Rosalia Pasca Jan 2011

Analogous Mechanisms Of Resistance To Benzothiazinones And Dinitrobenzamides In Mycobacterium Smegmatis, Ana Luisa Ribeiro, Giulia Degiacomi, Fanny Ewann, Silvia Buroni, Maria Loreto Incandela, Laurent R. Chiarelli, Giorgia Mori, Jaeseung Kim, Monica Contreras-Dominguez, Young-Sam Park, Sung-Jun Han, Priscille Brodin, Giovanna Valentini, Menico Rizzi, Giovanna Riccardi, Maria Rosalia Pasca

Faculty and Staff Publications

Tuberculosis is still a leading cause of death worldwide. The selection and spread of Mycobacterium tuberculosis multidrug-resistant (MDR-TB) and extensively drug-resistant strains (XDR-TB) is a severe public health problem. Recently, two different classes of chemical series, the benzothiazinones (BTZ) and the dinitrobenzamide (DNB) derivatives have been found to be highly active against M. tuberculosis, including XDR-TB strains. The target of BTZs is DprE1 protein which works in concert with DprE2 to form the heteromeric decaprenylphosphoryl-β-D-ribose 2'-epimerase, involved in Decaprenyl-Phospho-Arabinose (DPA) biosynthesis. Interestingly, it has been shown that the DNBs block the same pathway thus suggesting that both drugs could share …

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Mapping And Modeling Of Neglected Tropical Diseases In Brazil And Bolivia, Paula Mischler Jan 2011

Mapping And Modeling Of Neglected Tropical Diseases In Brazil And Bolivia, Paula Mischler

LSU Doctoral Dissertations

Accurately defining disease distributions and calculating disease risk is an important step in the control and prevention of diseases. This study used geographical information systems and remote sensing technologies within the MaxEnt ecological niche modeling program to create predictive risk maps for leprosy and Schistosomiasis in Brazil and Chagas disease in both Brazil and Bolivia. New disease cases were compiled for leprosy, Schistosomiasis, and Chagas disease from the Brazilian ministry of Health for 2001 to 2009 and the data was stratified to a 10,000 population for each municipality. Bolivian Chagas prevalence rates were calculated from 2007 to 2009 survey data. …

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Investigating Early Childhood Teachers' Stress And Social Supports: A Multi-Methods Approach, Ashley Diane Romero Jan 2011

Investigating Early Childhood Teachers' Stress And Social Supports: A Multi-Methods Approach, Ashley Diane Romero

LSU Master's Theses

Previous research has indicated that teaching is a high stress profession and that social supports may help to mediate teacher job stress. Ecological maps have been used in both research and other fields in order to study individuals’ social networks. Using a sample of ten Pre-K teachers, this study aims to answers two questions of inquiry: 1) Is the amount of reported social supports for Pre-K teachers negatively associated with reported stress?, and 2) What information does the eco-map interview give us about teacher’s social supports and stress? A multi-methods approach of both qualitative and quantitative techniques was used in …

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Development And Application Of Serogroup-Independent And Serogroup-Specific Loop-Mediated Isothermal Amplification Assays For Detecting Shiga Toxin-Producing Escherichia Coli, Fei Wang Jan 2011

Development And Application Of Serogroup-Independent And Serogroup-Specific Loop-Mediated Isothermal Amplification Assays For Detecting Shiga Toxin-Producing Escherichia Coli, Fei Wang

LSU Doctoral Dissertations

Shiga toxin-producing Escherichia coli (STEC), encompassing E. coli O157:H7 and non-O157 STEC, is a significant cause of foodborne illnesses and deaths in the United States and worldwide. Shiga toxins (encoded by stx) and intimin (encoded by eae) are important virulence factors for STEC strains causing infection. Although E. coli O157:H7 remains to be the single most common STEC causing disease, the clinical importance of non-O157 STEC is on the rise worldwide. And six major serogroups (O26, O45, O103, O111, O121, and O145) accounted for over 70% of non-O157 STEC infections in the United States. Loop-mediated isothermal amplification (LAMP) is a …

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Fully Integrated Microsystem For Bacterial Genotyping, Hui-Wen Chen Jan 2011

Fully Integrated Microsystem For Bacterial Genotyping, Hui-Wen Chen

LSU Doctoral Dissertations

Methods for bacterial detection and identification has garnered renewed interest in recent years due to the infections they may cause and the antimicrobial resistances they can develop, the potential for bioterrorism threats and possible contamination of food/water supplies. Therefore, the rapid, specific and accurate detection of pathogens is crucial for the prevention of pathogen-related disease outbreaks and facilitating disease management as well as the containment of suspected contaminated food and/or water supplies. In this dissertation an integrated modular-based microfluidic system composed of a fluidic cartridge and a control instrument has been developed for bacterial pathogen detection. The integrated system can …

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Arbovirus Phenotype Alters Transmission Potential, Rebecca C. Christofferson Jan 2011

Arbovirus Phenotype Alters Transmission Potential, Rebecca C. Christofferson

LSU Doctoral Dissertations

Extrinsic and environmental factors are known to affect the transmission of arthropod-borne viruses (arboviruses), including variations in the arthropod vector populations. Differences among these factors have been associated with differential transmission and are sometimes used to control the spread of an arbovirus through a vertebrate population in an effort to prevent or disrupt an outbreak. However, diversity in intrinsic viral populations, such as genetic and phenotypic variability, is not often accounted for when considering alterations in transmission. Presented in this dissertation are four experimental studies that explore the contribution of viral intrinsic factors, especially phenotypic variability, to the transmission potential …

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An Examination Of Oral Fed Post-Trauma Patients For Calorie And Protein Consumption As A Percentage Of Estimated Needs, And How It Impacts Length Of Hospital Stay And Wound Healing, Jamie Marchio Jan 2011

An Examination Of Oral Fed Post-Trauma Patients For Calorie And Protein Consumption As A Percentage Of Estimated Needs, And How It Impacts Length Of Hospital Stay And Wound Healing, Jamie Marchio

UNF Graduate Theses and Dissertations

Due to rising costs of healthcare, clinicians are responsible for decreasing costs by providing care that moves the patient from injury, to recovery, to discharge within a matter of just a few days. The Registered Dietitian is responsible for the nutrition assessment and nutrition diagnosis, nutrition prescription and plan, and for monitoring the patient to achieve nutritional repletion within this short time frame. Concerns for patients who require nursing staff and caregivers for feeding are evident, especially when increased demands are being placed on the nursing staff. This study aims to determine whether or not “feeder patients” are at a …

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Food-Borne Ultimatum: Proposing Federal Legislation To Create Humane Living Conditions For Animals Raised For Food In Order To Improve Human Health, The, Lynn M. Boris Jan 2011

Food-Borne Ultimatum: Proposing Federal Legislation To Create Humane Living Conditions For Animals Raised For Food In Order To Improve Human Health, The, Lynn M. Boris

Journal of Law and Health

In order to reduce the large number of human health risks associated with reckless farming practices, Congress must enact federal legislation that requires humane living conditions for farm animals and declares a moratorium on the routine use of unnecessary antibiotics. Part II of this Note will briefly review traditional farming and animal husbandry practices and examine the shift to the modern practices used by producers of animal products today. Part II will also present several farming practices utilized today that are particularly dangerous to human health. Part III of this Note will explore the immense human suffering that is occurring …

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Disease, Medicine And Health, Diego Armus, A. López Denis Jan 2011

Disease, Medicine And Health, Diego Armus, A. López Denis

History Faculty Works

This article focuses on three overlapping trends in the historical study of human responses to illness, labeled as “new history of medicine, history of public health, and sociocultural history of disease.” The topics range from colonial epidemiology and pharmacopoeia to twentieth-century public health institutions and urban hygiene. But a consistent focus on the social, cultural, and symbolic components of diseases and cures unifies this historiography and distinguishes it from the narrower scope of the long-established field of the history of medicine.

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Outlook Magazine, Spring 2011 Jan 2011

Outlook Magazine, Spring 2011

Outlook Magazine

No abstract provided.

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Humboldt Journal Of Microbiology: Volume 13, Number 1- May 2011, Press Humboldt Jan 2011

Humboldt Journal Of Microbiology: Volume 13, Number 1- May 2011, Press Humboldt

Humboldt Journal of Microbiology

No abstract provided.

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Malaria, Intestinal Parasitic Infection, Anemia, And Malnourishment In Rural Cameroonian Villages With An Assessment Of Early Interventions, D. J. Richardson, K. R. Richardson, K. E. Richardson, J. Gross, P. Tsekeng, B. Dondji, S. Foulefack Jan 2011

Malaria, Intestinal Parasitic Infection, Anemia, And Malnourishment In Rural Cameroonian Villages With An Assessment Of Early Interventions, D. J. Richardson, K. R. Richardson, K. E. Richardson, J. Gross, P. Tsekeng, B. Dondji, S. Foulefack

Journal of the Arkansas Academy of Science

Malaria, water-borne diarrheal diseases, and geohelminth infections, combined with severe malnutrition ravage entire villages throughout subSaharan Africa. The Bawa Health Initiative (BHI) is a 501c(3) non-profit organization with the goal of implementing a comprehensive public health program in an attempt to address these problems in a series of rural villages located in the West Province of Cameroon, Africa. Interventions include the provision of permethrin-treated bed nets to reduce the transmission of malaria, the installation of biosand water filters to reduce the prevalence of water-borne diseases, and a geohelminth control program utilizing mass treatment with albendazole. This study details the results …

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Ambivalence And Activism: Employment Discrimination In China, Timothy Webster Jan 2011

Ambivalence And Activism: Employment Discrimination In China, Timothy Webster

Vanderbilt Journal of Transnational Law

Chinese courts have not vigorously enforced many human rights, but a recent string of employment discrimination lawsuits suggests that, given the appropriate conditions, advocacy strategies, and rights at issue, victims can vindicate constitutional and statutory rights to equality in court. Specifically, carriers of the hepatitis B virus (HBV) have used the 2007 Employment Promotion Law to ground legal challenges against employers who discriminate against them in the hiring process. Plaintiffs' relatively high success rate suggests official support for making one prevalent form of discrimination illegal. Central to these lawsuits is a broad network of lawyers, activists, and scholars who actively …

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Three Structural Changes For A New System Of International Climate Change Mitigation Agreements Based On The Wto Model, Samara Spence Jan 2011

Three Structural Changes For A New System Of International Climate Change Mitigation Agreements Based On The Wto Model, Samara Spence

Vanderbilt Journal of Transnational Law

Past policy approaches to achieving international climate change mitigation have restricted the means for achieving mitigation to broad emissions caps. These policies have ignored the true nature of the climate change mitigation problem and have failed. This Note proposes a new design for a climate change mitigation system. It begins by analyzing the basic assumptions of the current cursory approach and by reviewing structural problems with those assumptions. It then reviews the successful World Trade Organization (WTO) model as a possible alternative structure and uses realities of the climate change problem to show why such an alternative could work in …

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Improving The Influenza Vaccination Rate Of Health Care Professionals In Long Term Care, Linda Laughlin Jan 2011

Improving The Influenza Vaccination Rate Of Health Care Professionals In Long Term Care, Linda Laughlin

Doctor of Nursing Practice (DNP)

No abstract provided.

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Comparative Genomic Analysis Of Vibrio Cholerae And Its Colonization Factors / Cindy Teh Shuan Ju., Cindy Shuan Ju Teh Jan 2011

Comparative Genomic Analysis Of Vibrio Cholerae And Its Colonization Factors / Cindy Teh Shuan Ju., Cindy Shuan Ju Teh

Student Works (2010-2019)

Vibrio cholerae, the causative agent of cholera, is endemic in Malaysia and is spread through the ingestion of contaminated food or water. The aims of this study were to develop a multiple PCR assay for differentiation of V. cholerae from other Vibrio species; to compare the efficiency of PCR assay with conventional biochemical tests and API 20E.; to characterize the strains based on their biotypes, serogroups, and virulotype by using a multiplex PCR assay; to determine the genetic relatedness of strains by using genotyping methods such as RAPD-PCR, ERIC-PCR, REP-PCR, MLVA, PFGE, MLST and MVLST; to study the virulence factors …

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Manipulation Of Probiotics Fermentation Of Milk By Cinnamon Zeylanicum, Glycyrrhiza Glabra Or Allium Sativum And Their Effects On Inhibition Of Helicobacter Pylori Growth In Vitro / Sara Behrad, Sara Behrad Jan 2011

Manipulation Of Probiotics Fermentation Of Milk By Cinnamon Zeylanicum, Glycyrrhiza Glabra Or Allium Sativum And Their Effects On Inhibition Of Helicobacter Pylori Growth In Vitro / Sara Behrad, Sara Behrad

Student Works (2010-2019)

Dairy products containing probiotics (e.g. Lactobacillus ssp. and Bifidobacterium) and certain herbs have inhibitory effects on the growth of Helicobacter pylori. The objectives of the present study were to determine the effects of herbs traditionally used for the treatment of gastric ulcer on yogurt fermentation characteristics, probiotic bacteria and the growth of H. pylori in vitro. Cinnamon zeylanicum (cinnamon), Glycyrrhiza glabra (licorice) or Allium sativum (garlic) was individually mixed with milk and the mixtures were fermented by probiotic bacteria to form herbal-yogurts. Changes in pH, titratable acididity, anti oxidant activity and the viable cell count of Lactobacillus ssp. and Streptococcus …

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Differentiation Of Two Taxa Of Caulerpa Based On Tufa Marker / Maryam Dalili Shofaei, Maryam Dalili Shofaei Jan 2011

Differentiation Of Two Taxa Of Caulerpa Based On Tufa Marker / Maryam Dalili Shofaei, Maryam Dalili Shofaei

Student Works (2010-2019)

The Caulerpa is an economical important genus among chlorophyta, which was initially described by Lamouroux in 1809, since then some 70 species of Caulerpa have been identified. The Caulerpa species have complex morphology that provides some characters that can be used in taxonomy, but extensive plasticity makes species boundaries difficult to be defined. With advancement in the field of molecular biology, it provides some alternatives for better understanding of taxonomy. In this study, PCRbased molecular techniques were used in an attempt to differentiate two taxa of Caulerpa based on tufA marker. In this research work, specimens were collected from Pulau …

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Screening Selected Actinobacteria For Antifungal Activity / Ezzatul Syahadah Zainordin, Zainordin Ezzatul Syahadah Jan 2011

Screening Selected Actinobacteria For Antifungal Activity / Ezzatul Syahadah Zainordin, Zainordin Ezzatul Syahadah

Student Works (2010-2019)

Actinobacteria are well known for their production of an extensive array of chemically diverse and medically important secondary metabolites. Fifty two strains of actinobacteria used in this study were divided into 20 colour groups based on their aerial and substrate mycelium colour as well as diffusible pigment production on Yeast Extract Malt Extract (ISP) medium. Antifungal activity was determined against Candida albicans, Candida parapsilosis, Fusarium oxysporum, Ganoderma boninense, Schizosaccharomyces pombe and Saccharomyces cerevisiae by the agar plug-diffusion method. Ten of the fifty two actinobacterial strains showed strong antifungal activity. Two strains (MA04119 and RHRS) were active against S. pombe, four …

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Genetic Diversity Of Plasmodium Falciparum Isolated From Yemen Based On The Genes Of Merozoite Surface Proteins (Msp-1 And Msp-2) / Nazeh Mohammed Al-Abd Ali, Nazeh Mohammed Al-Abd Ali Jan 2011

Genetic Diversity Of Plasmodium Falciparum Isolated From Yemen Based On The Genes Of Merozoite Surface Proteins (Msp-1 And Msp-2) / Nazeh Mohammed Al-Abd Ali, Nazeh Mohammed Al-Abd Ali

Student Works (2010-2019)

Malaria is a major health problem causing substantial morbidity and mortality with Plasmodium falciparum being the causative agent of the most severe and deadly form of malaria in humans. In Yemen Malaria is one of the most serious health problems. About 60% of the populations live in areas with malaria transmission. P. falciparum is the predominant species and accounts for more than 90% of malaria cases. Information on the nature and extent of genetic diversity within P. falciparum is essential for understanding the mechanism underlying the pathology of malaria, the acquisition of immunity, the spread of drug resistance and the …

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Antioxidant And Cytotoxic Investigations Of Beta Vulgaris L / Jaime Stella A/P Moses Richardson, Jaime Stella Moses Richardson Jan 2011

Antioxidant And Cytotoxic Investigations Of Beta Vulgaris L / Jaime Stella A/P Moses Richardson, Jaime Stella Moses Richardson

Student Works (2010-2019)

Beta vulgaris has been commonly consumed and traditionally used for various medicinal purposes. The present study scientifically evaluates the antioxidant and cytotoxic potential of the plant using various bioassays. The root part of Beta vulgaris was collected and extracted using methanol and then fractionated with hexane, ethyl acetate and water. The fresh root of Beta vulgaris was juiced and then subjected to evaporation under reduced pressure to form an extract. Chemical investigations were then directed to the ethyl acetate fraction and juice extract; fraction ET1, ET2 and ET3 were obtained from ethyl acetate fraction by isolation using high performance liquid …

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Design And Synthesis Of A Potential Inhibitor For Den2 Ns2b/Ns3 Serine Protease / Lee Yean Kee, Yean Kee Lee Jan 2011

Design And Synthesis Of A Potential Inhibitor For Den2 Ns2b/Ns3 Serine Protease / Lee Yean Kee, Yean Kee Lee

Student Works (2010-2019)

This work involves searching and designing of inhibitors for DEN2 NS2B/NS3 serine protease. It comprises three phases: modeling, synthesis and screening. Homology model construction of DEN2 NS2B/NS3 serine protease was carried out using HCV NS3/NS4A as a template. The model was then evaluated using server-based structural verification from UCLA-DOE Institute for Genomics and Proteomics server (http://nihserver.mbi.ucla.edu/SAVES/) and PROCHECK, VERIFY3D and ERRAT. The results revealed the homology model have reasonable protein fold compared to the crystal structure of DEN2 NS3 without the cofactor of NS2B bound within. The work then continued with in silico protein-ligand docking experiment using AUTODOCK 3.05, where …

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Biological Activities Of Penicilliopsis Sp. / Nadia Iryani Binti Najri, Najri Nadia Iryani Jan 2011

Biological Activities Of Penicilliopsis Sp. / Nadia Iryani Binti Najri, Najri Nadia Iryani

Student Works (2010-2019)

This study was carried out to evaluate the biological activities of Penicilliopsis sp. (KUM60280). The methanol and dichloromethane crude extracts were evaluated for their antimicrobial, antioxidant, cytotoxic and anti-human papillomavirus (HPV) activities. The amounts of total phenolic compound in the extracts were also evaluated. The antibacterial capacity was screened against Gram positive bacteria (Bacillus subtilis, B. cereus, Staphylococcus aureus, Streptococcus mutans, S. mitis, S. sanguis) as well as Gram negative bacteria (Escherichia coli, Pseudomonas auruginosa and P. vulgaris). Antifungal potentials on the other hand, were evaluated against Candida albicans, C. parasilopsis and Saccharomyces pombe by the disc diffusion method. The …

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Isolation And Purification Of A Bioactive Protein From Indigenous Basidiomycete Ganoderma Australe (Fr.) Pat / Nurulaina Binti Saidin, Nurulaina Saidin Jan 2011

Isolation And Purification Of A Bioactive Protein From Indigenous Basidiomycete Ganoderma Australe (Fr.) Pat / Nurulaina Binti Saidin, Nurulaina Saidin

Student Works (2010-2019)

The present study attempted to purify bioactive protein from mycelia of Ganoderma culture using organic extraction method (dichloromethane and 50% ethanol) and selection of chromatographic techniques. By using crude ethanolic extract, four strains of indigenous Ganoderma australe and one from Ganoderma tsugae were assessed for their antagonistic activity against human pathogenic bacteria and yeast. All Ganoderma strains tested exhibited different levels of antibacterial activity against both Gram-positive and Gram-negative bacteria. The ethanolic extract from G. australe strain KUM60813 was selected for further protein purification on the basis of intense antibacterial activity compared to proteins from other strains tested. The protein …

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