Digital Commons Network^™

Navigating The Global Health Terrain: Mapping Global Health Diplomacy, David Fidler

Articles by Maurer Faculty

This article engages in mapping thinking and practice on global health diplomacy. Increased interest in “global health diplomacy” and “health diplomacy” heightens the need for more rigorous descriptive, conceptual, analytical, and practical approaches to these phenomena. This article discusses why more rigor is needed with respect to global health diplomacy, provides a way to describe global health diplomacy that provides a foundation for further analysis, explores conceptual underpinnings of global health diplomacy to deepen the mapping exercise, and offers a simple but flexible analytical template for use in mapping different aspects of global health diplomacy. The article concludes with thoughts …

Thoughts In A Disaster, Sean Kivlehan

Quill & Scope

This paper discusses Sean’s experiences in Haiti during his psychiatry clerkship.

Military Forces, Global Health, And The International Health Regulations (2005), David P. Fidler

Articles by Maurer Faculty

Security, economic, development, and humanitarian threats created by infectious diseases have heightened the importance of military forces to national and global public health responses. This article explores the increasing need for military involvement in public and global health surveillance and response to infectious disease threats, and focuses on how military forces can more effectively support implementation of the World Health Organization’s International Health Regulations (2005) (IHR (2005)). The article explains the major changes made in negotiations that produced the IHR (2005) and the importance of these changes to military-to-military activities and civilian-military cooperation. It identifies five areas in which military …

Demography, Paleopathology, And Health Status Of The Moche Remains In Huambacho, Peru: A Comprehensive Osteological Analysis, Emily Grace

LSU Master's Theses

The Moche civilization, which thrived in northern Peru from AD 100-800, influenced a vast area that extended to the southernmost monumental site of Pañamarca. Huambacho, an archaeological site in the Nepeña Valley, which dates to the Early Horizon (600-200 cal BC), has yielded multiple Moche graves uncovered by the Proyecto Huambacho (2003-2004). The graves provide the first evidence of Moche presence outside of the neighboring Pañamarca monumental complex. This research entailed the osteological analysis of eleven individuals from nine Moche grave contexts at Huambacho, including the analysis of demographic features, pathology, trauma, and antemortem cultural modifications. Methodology included the visual …

Arginine Metabolism In The Edwardsiella Ictaluri- Channel Catfish Macrophage Dynamic, Wes Arend Baumgartner

LSU Doctoral Dissertations

Edwardsiella ictaluri encodes a urease operon and an arginine decarboxylase (AdiA) that are required for virulence in head kidney derived macrophages (HKDM). The urease produces ammonia in amounts sufficient to alter environmental pH from acid to neutral. A hypothetical model was proposed, involving arginine metabolism in E. ictaluri infected HKDM, focusing on bacterial urease, AdiA, a second arginine decarboxylase (SpeA), and agmatinase (SpeB). Using fluorescence based ratiometric pH determination of E. ictaluri in live HKDM, it was shown that E. ictaluri modulates HKDM phagosome pH to above six. Urease and AdiA mutants failed to up-regulate vacuole pH, while vacuole pH …

Environmental Factors Impacting Coastal Beach Health, Kevin Michael Chenier

LSU Master's Theses

The objectives of this research were to 1) determine the variability of fecal-indicator bacteria in Gulf of Mexico marine waters, 2) determine the effect of environmental variables on enterococci concentrations, 3) determine if beach sand at Holly Beach, LA was capable of acting as a reservoir for fecal-indicator bacteria, and 4) determine if a model could be developed to reduce the time it takes to issue a beach swim advisory. Fecal-indicator bacteria concentrations varied from year to year in historical data collected from a Gulf Coast beach. Afternoon fecal-indicator bacteria concentrations were consistently lower than morning concentrations, demonstrating daily variability. …

Inhibitory Effects Of Berry Anthocyanins On Palmitic Acid- Or Lipopolysaccharide- Induced Inflammation In Human Preadipocytes, Chenfei Gao

LSU Master's Theses

Obesity is an inflammatory disease associated with the development and progression of chronic degenerative diseases including insulin resistance, cardiovascular disease (CVD), type 2 diabetes mellitus (T2DM), and cancer. Circulating free fatty acid (FFA) are known risk factors promoting inflammation that can lead to CVD. Pre/adipocytes are known storage of low grade inflammatory biomarkers associated with obesity. However, the effects of dietary anthocyanins on inflammatory biomarkers in preadipocytes have not been reported. Berries are rich sources of naturally occurring antioxidant polyphenolics. There is increasing interest in the ability of berry anthocyanins to provide health benefits against obesity. Anthocyanins were isolated from …

Use Of Condensed Tannins Supplementation And Inorganic Copper As Deworming Agents In Naturally Infected Ewes And Lambs, Moara De Santana Martins

LSU Master's Theses

Sheep production is an important socioeconomic activity for the small producers and it can represent their primary income source. Gastrointestinal parasites have become the principal limiting factor for this industry around the world, especially because of the anthelmintic resistance phenomenon that has been increasing worldwide. The negligent use of anthelmintics has been reported as the main factor in the development of resistance. Among the parasites that infect sheep, H. contortus is considered the most harmful and also the parasite responsible for the fastest development of nematode resistance in small ruminants. Due to the economic impact this parasite may bring upon …

Integrated Modular Microfluidic System For Forensic Alu Dna Typing, Samuel Kimani Njoroge

LSU Doctoral Dissertations

Driven by the numerous applications of genome-related research, fully integrated microfluidic systems have been developed that have advanced the capabilities of molecular and, in particular, genetic analyses. A brief overview on integrated microfluidic systems for DNA analysis is given in Chapter 1 followed by a report on micro-capillary electrophoresis (µCE) of Alu elements with laser-induced fluorescence (LIF) detection, in which the monomorphic Alu insertions on the X and Y chromosomes were utilized to detect male DNA in large female DNA background (Y: X = 1:19) without cell sorting prior to the determination. The polymorphic Alu loci with known restricted geographical …

Rit Influenza Virus Database And Evidence For Differential Selection Pressures On Geographically Distinct Human Influenza A/H3n2 Virus Populations, Bindhu Satheeshkumar

Theses

Increasing amounts of data available from influenza virus genome sequencing projects have necessitated the need for centralized repositories for the collection, integration, and dissemination of these data to researchers for further research. The RIT Influenza Virus Database, a complete genome database for influenza viruses, has been created with a web interface so that users could query for viruses of interest and download complete genome sequences and metadata of isolates. The interface of the database is publicly accessible at http://bucatini.bioinformatics.rit.edu/bindhuURMC/. Even though it is known that influenza viruses are under constant evolution at the surface glycoproteins to develop drug resistance or …

Between "Voluntary Migrants" And War Refugees: The Health Of The Shan Burmese Migrant Workers In Northern Thailand, Nishant Verma, Celina Su, Coralie Chan, Peter Muennig

Publications and Research

The Shan are a large migrant group from Burma (Myanmar) found within Thailand. While some Shan migrate to escape the civil war within Burma, others migrate for economic opportunity. Despite the size of this population, little is known about their health. Our study highlights the need for expanded access to primary care among this Shan population. Despite the arduous and trying journeys of these Shan migrants, they do not display the level of health sometimes attributed to health selection among immigrant groups.

The Role Of Hiv-1 Proteins In Alzheimer's Disease Pathology, Brian Nelson Giunta

USF Tampa Graduate Theses and Dissertations

Prevalence of HIV-associated cognitive impairment is rising, the worst form of which is HIV-associated dementia (HAD). The disease is fuiled by a chronic innate type pro-inflammatory response in the brain which is highly dependent upon the activation of microglia. We first created an in vitro model of HAD composed of cultured microglial cells synergistically activated by the addition of IFN-gamma and the HIV-1 coat glycoprotein, gp120. This activation, as measured by TNF-alpha and NO release, is synergistically attenuated through the alpha7nAChR and p44/42 MAPK system by pretreatment with nicotine, and the cholinesterase inhibitor, galantamine. As these medications have been FDA …

Β-Lactams Interfering With Pbp1 Induce Panton-Valentine Leukocidin Expression By Triggering Sara And Rot Global Regulators Of Staphylococcus Aureus, Oana Dumitrescu, Priya Choudhury, Sandrine Boisset, Cedric Badiou, Michele Bes, Yvonne Benito, Christiane Wolz, Francois Vandenesch, Jerome Etienne, Ambrose L. Cheung

Dartmouth Scholarship

Previous articles reported that beta-lactam antibiotics increase the expression of Staphylococcus aureus Panton-Valentine leukocidin (PVL) by activating its transcription. We investigated the mechanisms underlying the inductor effect of beta-lactams on PVL expression by determining targets and regulatory pathways possibly implicated in this process. We measured PVL production in the presence of oxacillin (nonselective), imipenem (penicillin-binding protein 1 [PBP1] selective), cefotaxime (PBP2 s

Synthesis And Antimicrobial Activities Of S,S'-Heterosubstituted Disulfides, Praveen Ramaraju

USF Tampa Graduate Theses and Dissertations

Antibiotic resistance is a particularly critical health concern and has increased dramatically over the past two decades. For over a decade the Turos laboratory has been designing small molecules to target pathogenic microbes such as Staphylococcus aureus and the resistant variants like methicillin-resistant Staphylococcus aureus (MRSA). Previously, N-thiolated Β-lactams, N-thiolated 2-oxazolidinones and aromatic disulfides that were synthesized in Dr. Turos' lab have shown strong activity against these bacteria. The present work describes the synthesis and antimicrobial activities of a related structural class called S,S'-heterosubstituted disulfides. For ages, sulfur (elemental) has been used as an antibacterial for controlling infestation and bacterial …

Generation And Application Of Antigen-Specific Induced Regulatory T Cells In Allogeneic Bone Marrow Transplantation, Kenrick Semple

USF Tampa Graduate Theses and Dissertations

CD28 co-stimulation is required for the generation of naturally occurring regulatory T cells (nTregs) in the thymus through Lck-signaling. However, high level of CD28 suppresses the generation of induced Tregs (iTregs) from naïve CD4 T cells, although underlying mechanism(s) has not been defined. Here we investigated the role of CD28-mediated signaling pathways in the suppression of Treg generation. We used a series of transgenic (Tg) mice on CD28-deficient background that bears WT CD28 or mutated CD28 in its cytosolic tail incapable of binding to Lck, PI3K or Itk. Regardless of exogenous IL-2, strong CD28 costimulation suppressed iTreg generation through Lck …

Novel Roles For The Transcriptional Repressor Prdm1 In Human Natural Killer Cells And Identification Of An Inhibitor Of Its Interacting Methyltransferase G9a, Matthew Adams Smith

USF Tampa Graduate Theses and Dissertations

The studies presented within this dissertation provide the first description of PRDM1 (also known as Blimp-1 or PRDI-BF1) function in natural killer cells. NK cells are major effectors of the innate immune response via antigen-independent cytotoxicity and link to the adaptive immune response through cytokine release. Molecular mechanisms mediating NK activation are relatively well-studied; however, much less is known about the mechanisms that restrain activation.

In the first study, the transcriptional repressor PRDM1 is shown to be a critical negative regulator of NK function. Microarray analysis was used to characterize transcriptional changes associated with cytokine-mediated activation. PRDM1 is expressed at …

Growing Up With Hiv: Disease Management Among Perinatally Infected Adolescents, Barbara J. Szelag

USF Tampa Graduate Theses and Dissertations

Children born with HIV in the 1980s and 1990s are surviving into adolescence and adulthood, due to the availability of highly active antiretroviral therapy (HAART). Growing up with a chronic and stigmatized disease presents considerable challenges as young people explore their sexuality, develop relationships, and take steps to become independent and productive adults. Adherence to HAART is an essential and life-long practice for the maintenance of health and longevity. For adolescents born with HIV, a daily medication schedule is one aspect of disease management that also includes medical visits, HIV status acceptance, bouts of illness, and disclosure of HIV status …

Assessment Of Fertility Potential In Bottlenose Dolphins (Tursiops Truncatus): An Elisa-Based Biomarker Analysis, Leslie Schwierzke Wade

USF Tampa Graduate Theses and Dissertations

As apex predators in coastal systems, bottlenose dolphins (Tursiops truncatus) are susceptible to persistent organic pollutant (POP) accumulation and retention over time, which has prompted continued interest in understanding the extent to which contaminant body burdens or other stressors are sufficient to cause adverse sublethal effects on energetic fitness, immune function, or reproduction. Increasing our knowledge of reproductive endocrinology in bottlenose dolphins may provide insight into changes in reproductive rates, thereby expanding the capacity to assess conservation status. This study used the Enzyme-Linked ImmunoSorbent Assay (ELISA) technique to examine peptide fertility hormones [inhibin A, inhibin B and anti-Müllerian …

Sharps Injuries In Medical Training: Higher Risk For Residents Than For Medical Students, Rachel Williams

USF Tampa Graduate Theses and Dissertations

Because of their relative inexperience in performing procedures and handling sharps devices, medical students and resident physicians are considered to be at high risk for sharps injuries. A higher rate of sharps injuries for medical trainees implies a higher risk for occupationally-acquired infection with bloodborne pathogens and may have financial and legal implications for training institutions. This study examines the prevalence of sharps injuries among US medical students and resident physicians. A systematic review of the literature yielded 10 studies that gave data on sharps injuries for US medical students or residents, and those data were combined with data from …

Assessing The Relationship Of Monocytes With Primary And Secondary Dengue Infection Among Hospitalized Dengue Patients In Malaysia, 2010: A Cross-Sectional Study, Benjamin Glenn Klekamp

USF Tampa Graduate Theses and Dissertations

Dengue, a group of four similar viruses transmitted through the bite of a mosquito, is estimated to infect upwards of 100 million annually in over 100 nations throughout the global equatorial belt. Distribution of global dengue is highly skewed as Southeast Asian and Western Pacific regions endure 75% of the global dengue burden. Similar to other regional countries, Malaysia has been rapidly urbanizing, which has supported a hyperendemic dengue state.

The biological pathway by which dengue infection causes a wide range of clinical manifestations, spanning asymptomatic to life-threatening severe complications, is not comprehensively understood. Historically, severe dengue complications have primarily …

Evaluating The Get Into Fitness Today (Gift) Program: Weight Loss And The Roles Of Education And Empowerment, Emily Koby Novicki

USF Tampa Graduate Theses and Dissertations

This study investigated the effects of Get Into Fitness Today (GIFT), a health program for adults that promotes balanced nutrition, physical activity, and weight loss through weekly meetings that provide education and social support. In addition to investigating the effects of GIFT, this evaluation sought to better understand explanatory models used by participants and program staff, and the roles of education and empowerment in weight loss and health education. This mixed methods, case study evaluation consisted of quantitative analysis of existing program records for 664 participants, 40 hours of participant observation of class sessions with ten GIFT groups, and follow-up …

Environmental Health In The Latin American And Caribbean Region: Use Of Water Storage Containers, Water Quality, And Community Perception, Erlande Omisca

USF Tampa Graduate Theses and Dissertations

Water quality and availability are important issues in many developing countries where portions of populations still lack access to potable water. Throughout the English-speaking Caribbean and parts of Latin America, households and businesses invest in water supply systems even when they are connected to and pay for water services from a private or state owned provider. Inconsistent supplies of water from the water companies have led many people to invest in storage tanks which, if operated correctly, can provide water throughout the day even when the supply from the main is low or zero. While these individual systems help to …

Surface Functionalization And Analysis Thereof For An Ovarian Cancer Diagnostic Biosensor, Asad Ali Ahmad

USF Tampa Graduate Theses and Dissertations

Ovarian cancer is the fifth leading cause of cancer death among women in United States and has an alarming 1.4% (1 in 71) lifetime risk. The lack of overt symptoms and the absence of a reliable screening test to detect ovarian cancer result in over 70% of women being diagnosed after the disease has spread beyond the ovary resulting in a poor prognosis. A key characteristic of ovarian cancer is the ability of tumor cells to evade apoptosis, or programmed cell death contributing to the limitless replicative potential, which is a hallmark of all carcinogenesis. There is conclusive evidence that …

Development Of A Non-Invasive Electrode For Intradermal Electrically Mediated Dna Vaccination, Amy Lynn Donate

USF Tampa Graduate Theses and Dissertations

Current progress in the development of vaccines has decreased the incidence of fatal and non-fatal infections and increased longevity. However, new technologies need to be developed to combat an emerging generation of infectious diseases. DNA vaccination has been demonstrated to have great potential for use against a wide variety of diseases. Alone, this vaccine technology does not generate a significant immune response for vaccination, but combined with delivery by electroporation (EP), can enhance plasmid expression and immunity against the expressed antigen. Most EP systems, while effective, can be invasive and painful making them less desirable for use in vaccination. Our …