Digital Commons Network^™

Rapid Communication Rescue Of Hantaan Virus Minigenomes, Kirsten Flick, Connie S. Schmaljohn, Ralf F. Petterson, Heinz Feldmann, Ramon Flick, Jay Hooper

United States Army: Publications

Hantavirus infections are a major public health concern worldwide. Their widespread geographical distribution and their ability to produce serious, often fatal, human disease underline the need for a system that allows manipulation of these viruses. We describe here the first successful establishment of a reverse genetics technology for Hantaan virus, the prototype of the genus Hantavirus. The system offers a unique opportunity to study the biology of hantaviruses, the pathogenesis of the diseases, and the efficacy of antiviral and prophylactic measures against hantavirus infections.

2003 Trafficking In Persons Report, U.S. Department Of State

Human Trafficking: Data and Documents

AS unimaginable as it seems, slavery and bondage still persist in the early twenty-first century. Millions of people around the world still suffer in silence in slave-like situations of forced labor and commercial sexual exploitation from which they cannot free themselves. Trafficking in persons is one of the greatest human rights challenges of our time. It is, as the International Labour Organization (ILO) points out, the “underside of globalization.”

Comparative And International Health Law, Timothy Stoltzfus Jost

Scholarly Articles

No abstract provided.

Clinical Issues Surrounding Once-Daily Aminoglycoside Dosing In Children, Chad A. Knoderer, Julie A. Everett, William F. Buss

Scholarship and Professional Work – COPHS

Aminoglycoside antibiotics are first-line treatment for many infectious diseases in the pediatric population and are effective in adults. The traditional dosing interval in children is every 8–12 hours. Studies in adults reported equivalent efficacy and equal or less toxicity with once-daily regimens. Despite many studies in the adult population, this approach has yet to become standard practice in most pediatric hospitals. Reasons for lack of acceptance of this strategy in children include rapid aminoglycoside clearance, unknown duration of postantibiotic effect, safety concerns, and limited clinical and efficacy data.

No More Kidding Around: Restructuring Non-Medical Childhood Immunization Exemptions To Ensure Public Health Protection, Ross D. Silverman

Annals of Health Law and Life Sciences

Professor Silverman's article examines the complex challenges faced by U.S. policymakers attempting to balance the public health protections of mandatory childhood immunization programs with the legal, religious, philosophical, and practical concerns raised by permitting non-medical exemptions under the programs. The article begins with a discussion of the history of childhood immunization programs, and continues by describing the inconsistency of enforcement of state immunization laws and exemptions. The author analyzes recent cases from New York, Wyoming, and Arkansas, and discusses how these decisions both pose threats to these programs' public health protections, while also offering insight into potential problems for other …

2003 Stethoscope, University Of Health Sciences College Of Osteopathic Medicine

Yearbooks (1927-2005)

Annual publication of the University of Health Sciences College of Osteopathic Medicine.

Print copy located in the Special Collections room in the D'Angelo Library.

Gyra Mutations In Ciprofloxacin-Resistant Bartonella Bacilliformis Strains Obtained In Vitro, Michael F. Minnick, Zachary R. Wilson, Laura S. Smitherman, D. Scott Samuels

Biological Sciences Faculty Publications

We isolated and characterized mutants of Bartonella bacilliformis that are resistant to the fluoroquinolone antibiotic ciprofloxacin, which targets the A subunit of DNA gyrase. Mutants had single point mutations in the gyrA gene that changed either Asp-90 to Gly or Asp-95 to Asn and had 3- or 16-fold higher resistance, respectively, to ciprofloxacin than did wild-type B. bacilliformis. Asp-95 is homologous to Asp-87 of Escherichia coli GyrA and is a common residue mutated in fluoroquinolone-resistant strains of other bacteria. This is the first report of a mutation at an Asp-90 homologue, which corresponds to Asp-82 in E. coli GyrA.

Regulation Of Type 1 Diabetes By A Self-Mhc Class Ii Peptide: Role Of Transforming Growth Factor Beta (Tgf-Beta)., Bhagirath Singh, R Mukherjee, P Chaturvedi, Babita Agrawal

Microbiology & Immunology Publications

The present study was undertaken to analyze the regulatory T cells generated in response to class I derived self-I-A beta(g7) (54-76) peptide. It was observed T cells from young unprimed type 1 diabetes (T1D) prone NOD mice did not respond to self-I-A beta(g7) (54-76) peptide although T cells from primed young NOD mice showed a strong response. T cells from young unprimed BALB/c mice responded to self-I-A beta(d) (62-78) peptide. However, a breakdown of tolerance to these peptides was observed with age in both the strains. Culture supernatant from I-A beta(g7) (54-76) peptide-primed cells secreted large amounts of TGF-beta and …

Prevalence Of The Use Of Central Venous Access Devices Within And Outside Of The Intensive Care Unit: Results Of A Survey Among Hospitals In The Prevention Epicenter Program Of The Centers For Disease Control And Prevention, Michael Climo, Dan Diekema, David K. Warren, Loreen A. Herwaldt, Trish M. Perl, Lance Peterson, Theresa Plaskett, Connie Price, Kent Sepkowitz, Steve Solomon, Jerry Tokars, Victoria J. Fraser, Edward Wong

Open Access Publications

No abstract provided.

Prevalence And Distribution Of Adhesins In Invasive Non-Type B Encapsulated Haemophilus Influenzae, Carina A. Rodriguez, Vasanthi Avadhanula, Amy Buscher, Arnold L. Smith, Joseph W. St. Geme Iii, Elisabeth E. Adderson

Open Access Publications

No abstract provided.

The Rova Mutant Of Yersinia Enterocolitica Displays Differential Degrees Of Virulence Depending On The Route Of Infection, Peter H. Dube, Scott A. Handley, Paula A. Revell, Virginia L. Miller

Open Access Publications

No abstract provided.

Differences In Percutaneous Injury Patterns In A Multi-Hospital System, Hilary M. Babcock, Victoria Fraser

Open Access Publications

No abstract provided.

Viral Discovery And Sequence Recovery Using Dna Microarrays, David Wang, Anatoly Urisman, Yu-Tsueng Liu, Michael Springer, Thomas G. Ksiazek, Dean D. Erdman, Elaine R. Mardis, Matthew Hickenbotham, Vincent Magrini, James Eldred, J P. Latreille, Richard K. Wilson, Don Ganem, Joseph L. Derisi

Open Access Publications

No abstract provided.

Invasive Disease Due To Nontypeable Haemophilus Influenzae Among Children In Arkansas, Joshua M. O'Neill, Joseph W. St. Geme Iii, David Cutter, Elisabeth E. Adderson, Juliana Anyanwu, Richard F. Jacobs, Gordon E. Schutze

Open Access Publications

No abstract provided.

Gene Discovery In The Apicomplexa As Revealed By Est Sequencing And Assembly Of A Comparative Gene Database, Deana Pape, John Martin, Todd Wylie, Mike Dante, Robert H. Waterston, Sandra W. Clifton, Et Al

Open Access Publications

No abstract provided.

Focus On: News Of Interest In The Photochemical Sciences, Volume 16 Issue 3, Spectrum Editors

The Spectrum

Includes: Patterning Photonic Crystals; Liquid Immersion Lithography; World's First Integrated Photonic Circuit; Photochemical Mercury Removal Process; Solar Surgery; Conferences Carbon Costs; Optical Manipulation: Tweezing Out a Revolution; Photosynthesis Genes Discovered in a Virus; A Steady Nanoglow; International Materials Institutes Grants; SARS Travel Restrictions

Classification By Race, Ethnicity, Color, Or National Origin.

Propositions

CLASSIFICATION BY RACE, ETHNICITY, COLOR, OR NATIONAL ORIGIN. INITIATIVE CONSTITUTIONAL AMENDMENT. • Amends Constitution to prohibit state and local governments from using race, ethnicity, color, or national origin to classify current or prospective students, contractors or employees in public education, contracting, or employment operations. Does not prohibit classification by sex. • Prohibition also covers persons subject to other operations of government unless Legislature finds compelling state interest, authorizes by two-thirds of each house, and Governor approves. • “Classifying” defined as separating, sorting, or organizing persons or personal data. Exemptions include: medical data; law enforcement descriptions; prisoner and undercover assignments; actions …

Expression Of Spot In Borrelia Burgdorferi During Serum Starvation, Marc B. Concepcion, David R. Nelson

Cell and Molecular Biology Faculty Publications

Borrelia burgdorferi, the causative agent of Lyme disease, is transmitted by the tick Ixodes scapularis. A 2.9-kb fragment containing a putative spoT gene was isolated from B. burgdorferi genomic DNA by PCR amplification and cloned into a pBAD24 vector. The cloned gene complemented Escherichia coli mutant strain CF1693, which contains deletions of both the relA and spoT genes. The spoT gene in E. coli encodes a bifunctional enzyme capable of synthesizing and degrading (p)ppGpp, which mediates the stringent response during carbon source starvation. B. burgdorferi has been reported to have a stress response to serum starvation. Thin-layer chromatography …

Hepp Report: Infectious Diseases In Corrections, Vol. 6 No. 1, Hiv & Hepatitis Education Prison Project

Infectious Diseases in Corrections Report (IDCR)

No abstract provided.

Enlt 495.80: From The Black Death To Aids, The Cultural And Scientific Impact Of Plagues, Herbert M. Swick

University of Montana Course Syllabi

No abstract provided.

Monte Carlo Assessments Of Goodness-Of-Fit For Ecological Simulation Models, Lance A. Waller, David Smith, James E. Childs, Leslie A. Real

Zoonotics and Wildlife Disease: Publications

One often develops stochastic ecologic simulation models based on local interactions between individuals or groups and bases systemic conclusions on trends summarized over multiple data sets generated from the model. In many cases, such models generate data sets (“realizations”) each violating the usual assumptions associated with traditional statistical tests of goodness-of-fit, most notably that of independent observations. Monte Carlo hypothesis tests applied to multiple realizations from such models provide appropriate goodness-of-fit tests regardless of within-model peculiarities. The Monte Carlo tests address the question “Do the observed data appear consistent with the model?” in contrast to the usual question “Does the …

Abundant PrpCwd In Tonsil From Mule Deer With Preclinical Chronic Wasting Disease, Katherine I. O'Rourke, Dongyue Zhuang, Amy Lyda, Gabriel Gomez, Elizabeth S. Williams, Wenbin Tuo, Michael W. Miller

Zoonotics and Wildlife Disease: Publications

A monoclonal antibody dot-blot assay was used to evaluate detergent lysates of tonsil tissue from mule deer to detect PrP^CWD, the marker for the cervid transmissible spongiform encephalopathy chronic wasting disease (CWD). Samples of formalin-fixed brain and tonsil tissues from mule deer were examined for PrP^CWD using immunohistochemistry (IHC) with Mab F99/97.6.1, the gold standard for diagnosis of preclinical CWD. The contralateral tonsil from each of the 143 deer was prepared for confirmatory IHC and as a 10% (wt/vol) detergent lysate without purification or enrichment steps for monoclonal antibody dot-blot assay. PrP^CWD was detected by dot-blot …

Comparison Of The Protective Efficacy Of Dna And Baculovirusderived Protein Vaccines For Ebola Virus In Guinea Pigs, Jenny Mellquist-Riemenschneider, Aura Garrison, Joan Geisbert, Kamal Saikh, Kelli Heidebrink, Peter Jahrling, Robert Ulrich, Connie Schmaljohn

United States Army: Publications

The filoviruses Ebola virus (EBOV) and Marburg virus (MARV) cause severe hemorrhagic fever in humans for which no vaccines are available. Previously, a priming dose of a DNA vaccine expressing the glycoprotein (GP) gene of MARV followed by boosting with recombinant baculovirus-derived GP protein was found to confer protective immunity to guinea pigs (Hevey et al., 2001. Vaccine 20, 568-593). To determine whether a similar prime-boost vaccine approach would be effective for EBOV, we generated and characterized recombinant baculoviruses expressing full-length EBOV GP (GP_1,2) or a terminally-deleted GP (GPa-) and examined their immunogenicity in guinea pigs. As expected, …

Washington University School Of Medicine Bulletin, 2003-2004

Washington University School of Medicine Bulletins

No abstract provided.

Reclaiming Civil Rights In Uncivil Times, Eric K. Yamamoto

UC Law Journal of Race and Economic Justice

Three Cs: Celebration, Caution, and Challenge. Three C's of both worry and hope. The first C is to celebrate the Civil Rights Movement's accomplishments, then and now-the innumerable ways as the movement broadened it has bettered the daily lives of communities of color and, indeed, all Americans. The second C is caution-to caution against the dismantling of civil rights fueled in part by the conservative New Federalism. The third C is challenge-to envision our role in justice struggles in the post-September 11th Bush-Ashcroft era and, specifically, to think hard about what "Civil Rights" now means and why "Reclaiming Civil Rights' …

Arrested Adults Awaiting Arraignment: Mental Health, Substance Abuse, And Criminal Justice Characteristics And Needs, Nahama Broner, Stacy S. Lamon, Damon W. Mayrl, Martin G. Karopkin

Fordham Urban Law Journal

This Study is one of the first to look at the mentally ill during the pre-arraignment process. The pre-arraignment process is an excellent place to identify individuals with mental health and substance abuse problems, to examine those problems, to consider legal interventions, such as diversion or routing to specialized courts, for instance, drug and mental health courts, and to plan for community mental health, substance abuse, health, and social service interventions. Following a brief review of the literature on rates of substance abuse and mental health problems for ciminal justice populations, the process from arrest to arraignment in Kings County …

A Priori Prediction Of Disease Invasion Dynamics In A Novel Environment, Colin A. Russell, David L. Smith, Lance A. Waller, James E. Childs, Leslie A. Real

Zoonotics and Wildlife Disease: Publications

Directly transmitted infectious diseases spread through wildlife populations as travelling waves away from the sites of original introduction. These waves often become distorted through their interaction with environmental and population heterogeneities and by long-distance translocation of infected individuals. Accurate a priori predictions of travelling waves of infection depend upon understanding and quantifying these distorting factors. We assess the effects of anisotropies arising from the orientation of rivers in relation to the direction of disease-front propagation and the damming effect of mountains on disease movement in natural populations. The model successfully predicts the local and large-scale prevaccination spread of raccoon rabies …

Pt 525.01: Clinical Medicine, Pharmacology And Exercise Prescription, Steve Fehrer, James J. Laskin

University of Montana Course Syllabi

No abstract provided.

Part 5: Research And Development At Eastern Virginia Medical School, Regional Studies Institute, Old Dominion University

State of the Region Reports: Hampton Roads

Our region’s medical school gradually has been developing a core of significant research and development thrusts despite tremendous challenges. What is EVMS doing and what can we do to stimulate this highly valuable activity?

Office Of Research And Graduate Studies -- Annual Report 2002/2003

Office of Research and Economic Development: Publications

Contents
01 Introduction
03 Nanomagnetics & Mesospin Structures
05 Redox Biology
07 Virology & Infectious Diseases
09 Bioprocessing & Vaccine Development
11 Plant Genomics
13 Complex Environmental Systems
15 Behavioral Sciences
17 Education in the 21st Century
19 Digital Texts & Contemporary Writing
21 Nanomanufacturing & Advanced Materials
23 Initiatives
25 Future
27 Graduate Studies
29 Technology Development
31 University of Nebraska Press
32 Financials
33 Inspiring Excellence