Digital Commons Network^™

Bone Microenvironmental Control Of Skeletal Malignancy, Chen Hao Lo

USF Tampa Graduate Theses and Dissertations

Bone is a common site of metastasis for many solid malignancies. Bone-metastatic cancers pose a significant clinical problem worldwide and is among the main causes for cancer patient morbidity and mortality. Patients with advanced bone-metastatic diseases often present with either osteolytic or osteogenic bone diseases as their cancers progress. These bone pathologies are products of the cancer co-opting the local bone remodeling stroma to yield important growth nutrients and factors. Unfortunately, skeletal metastases remain incurable and are fatal. Identifying and understanding the causal multicellular and molecular interactions underlying skeletal malignancies can yield crucial ideas for targeting and inhibiting disease progression. …

Investigating The Recent History Of A Changing Planet With Innovative Isotopic Techniques And New Geologic Archives, Ryan A. Venturelli

USF Tampa Graduate Theses and Dissertations

Globally averaged Earth surface temperatures indicate a 0.8°C warming since 1880. Though this warming is a result of anthropogenic influence that has exceeded natural forcings, a thorough understanding of Earth's climate system requires a knowledge of changes in global temperatures beyond the instrumental record. To achieve this, we must supplement the temporally limited observational record with proxy records of environmental conditions in the geologic past. Though the foundational questions underlying interrogations of the geologic past are seemingly simple (What happened? When?), the accuracy of their answers depend upon accessibility and availability of geologic materials as well as the capabilities of …

Formation Of Gas Jets And Vortex Rings From Bursting Bubbles: Visualization, Kinematics, And Fluid Dynamics, Ali A. Dasouqi

USF Tampa Graduate Theses and Dissertations

The bursting of bubbles at an air-liquid interface is an important physical process in many environmental and industrial applications. Bubble bursting has implications for climate (e.g. marine aerosol formation; Veron 2015), human respiratory health (e.g. aerosolization of harmful substances; Prather et al 2013), food science (e.g. beer and champagne bubbles; Liger-Belair et al 2009), industry (e.g. processes such as gas fluxing and electrowinning; Zhang et al 2011), and volcanology (e.g. Strombolian eruptions; Chojnicki et al 2015). Much of the previous research into bubble bursting has focused on the fluid dynamics of the liquid component, namely the bubble cap film rupture …

Using Quantitative Methods To Investigate Student Attitudes Toward Chemistry: Women Of Color Deserve The Spotlight, Guizella A. Rocabado Delgadillo

USF Tampa Graduate Theses and Dissertations

The field of Chemistry Education Research (CER) has been interested in understanding the reasons why students struggle in organic chemistry courses. Reports show that students perceive the material as difficult and have trouble keeping pace with the volume of content taught within the course. Beyond these and other explanations of why students struggle in organic chemistry are affective factors, such as attitude toward chemistry, that influence students’ success and retention in this course. Studies have shown that in many instances, underrepresented groups of students report less positive attitudes than their peers. Of greater concern are the students representing multiple marginalized …

Saudi Early Childhood Educators' Perceptions Of Gender Roles In Children's Dramatic Play, Dalal Alanazi

USF Tampa Graduate Theses and Dissertations

This study was conducted in a unique time of cultural and social change in Saudi Arabia. The new Saudi Vision 2030 promotes gender equality, and it is within this context that I investigated four Saudi preschool teachers’ perceptions of children’s gender roles in dramatic play. I focused on how the teachers have responded to the current move to think differently about gender roles in the Saudi context and the relevant policy changes. Rogoff’s sociocultural theory, which highlights the significance of cultural contexts on peoples’ perceptions, framed the study. I conducted semi-structured interviews with the four Saudi teachers. As a result …

Biosynthetic Gene Clusters, Microbiomes, And Secondary Metabolites In Cold Water Marine Organisms, Nicole Elizabeth Avalon

USF Tampa Graduate Theses and Dissertations

Microorganisms and invertebrate animals from cold water marine environments, such as in Antarctica and in Ireland’s deep sea, are a rich source of secondary metabolites. In this dissertation, research was centered around secondary metabolism and natural product biosynthesis. The projects include the delineation of biosynthetic gene clusters hypothesized to be responsible for the biosynthesis of the palmerolides, stratification of the microbiome of the Antarctic ascidian Synoicum adareanum, genomic and peptidomic analysis of a host-associated Antarctic Pseudovibrio sp., and isolation and characterization of secondary metabolites from the Irish deep-sea coral Drifa sp.

Palmerolide A, the principle secondary metabolite associated with S. …

Cognition In Health And Disease: Two Mouse Model Studies, Heather Lynn Mahoney

USF Tampa Graduate Theses and Dissertations

Cognitive and circadian dysfunction are common aspects of neuropsychiatric disease that are often overlooked in disease models and treatment strategies. In this work, we first evaluated the potential for cognitive enhancement in healthy adult mice by targeting a kinase involved in regulation of the molecular circadian clock. We then developed and characterized a model of neuropsychiatric disease that could present a target for rescuing circadian and cognitive deficits using this treatment.

Time-of-day effects have been noted in a wide variety of cognitive behavioral tests, and perturbation of circadian rhythms impairs hippocampus-dependent learning and memory. We first asked whether casein kinase …

Dendriplex - Cyclodextrin Conjugates For Gene Delivery To Retina, Durga Deepak Puro

USF Tampa Graduate Theses and Dissertations

Gene therapy holds promise for treating a wide range of diseases including cancer and several other polygenic and monogenic disorder arising in different organs. Due to its immune privilege nature, eye is an ideal organ for gene therapy,as various visual pathologies arise from gene defect and can lead to partial or complete vision loss. The last decade, several nanoparticles have been developed and tested for their transfection efficiency in vitro and in vivo. Among them, dendrimers with small size, good physicochemical properties and endosomal escape activity have shown high transfection efficiency in various cell lines. In an attempt to further …

Counseling Clients With Traumatic Brain Injury: Exploring Counselors’ Perceived Knowledge, Comfort, And Self-Awareness, Michelle Bradham-Cousar

USF Tampa Graduate Theses and Dissertations

The number of traumatic brain injury (TBI) diagnoses continues to rise each year. Counseling is a critical factor in TBI treatment, and although numerous studies have investigated TBI outcomes, a paucity of researchers have studied professional counselors’ knowledge, comfort, and self-awareness when working with TBI clients. Due to the diversity of counselor caseloads, it is likely that counselors will serve clients with a dual diagnosis that includes TBI. These dual diagnoses include depression, posttraumatic stress disorder, anxiety, psychosis, or another neurocognitive disorder. The purpose of this study was to explore counselors’ knowledge, comfort, and self-awareness when working with individuals with …

Data-Driven Modeling Of The Causes And Effects Of Interneuronal Dysfunction In Alzheimer’S Disease And Dravet Syndrome, Carlos Perez

USF Tampa Graduate Theses and Dissertations

One of the defining features of Alzheimer’s disease (AD) is the increased cleavage of the amyloid precursor protein (APP), causing abnormally high levels of the aggregation form of amyloid beta (Aβ ). Many studies have shown that both AD patients and AD mice models exhibit abnormal network activity, including hypersynchronous excitatory neuron behavior, altered brain rhythms, and in some instances epileptic seizures when exposed to high levels of Aβ In particular, strong experimental evidence suggests that it is the small globular amyloid oligomers (gOs) and curvilinear fibrils (CFs) rather than the more stable, late stage rigid fibrils (RFs) that cause …

Quantifying The Impact Of Chronic Stress On Racial Disparities In Cardiovascular Disease, Nnadozie Emechebe

USF Tampa Graduate Theses and Dissertations

Background: Despite declining mortality in cardiovascular diseases (CVD), racial disparities between non-Hispanic Blacks (NHB) and to non-Hispanic Whites (NHW) persist. Although the prevalence of traditional risk factors of CVD such as hypertension, is higher in NHB compared to NHW, adjusting for this difference does not eliminate the disparity completely. This suggests other factors might explain the persisting disparities. Thus, the purpose of this dissertation is to quantify the impact of chronic stress in explaining the racial disparities in cardiovascular diseases (CVD). This dissertation contains three studies that addressed the following Specific Aims: Specific aims: 1) To create and assess the …

Isolation Of Specific Phospholipids From Nannochloropsis Oculata Microalga For Cosmetic Applications, Ahmet Yener Manisali

USF Tampa Graduate Theses and Dissertations

Algae, photosynthetic aquatic organisms, have recently caught the attention of the food, cosmetic, pharmaceutical, and nutraceutical industries due to the variety of natural compounds in their cellular bodies such as carbohydrates, lipids, and proteins. Specifically, microalgae-derived natural compounds such as phospholipids are extensively utilized in cosmetics as part of liposome formers, emulsifiers, solubilizers, and wetting agents. Although phospholipids are currently extracted from food sources, this practice raises sustainability concerns. Hence, growing microalgae on the provision of macronutrients such as nitrates and phosphates will serve as a more sustainable source of the several specific phospholipids such as phosphatidylcholine (PC), phosphatidyl-ethanolamine (PE), …

Circadian Rhythms And The Embodiment Of Social Zeitgebers: Linking The Bio And Social, Tiffany R. Moore

USF Tampa Graduate Theses and Dissertations

Virtually all biological processes are under direct or indirect control of the circadian system. Chronic disruption of circadian rhythms, termed chronodisruption, is linked to increased risk for adverse health outcomes such as cancer, depression, and cardiometabolic disease. Circadian rhythms are highly sensitive to sociocultural contexts. As a result, circadian rhythms provide a valuable entryway to explore issues of embodiment. Here, embodiment refers to processes through which social experiences ‘get under the skin’ to become biological and manifest in health. To better understand what proximate social factors influence chronodisruption, this study assessed chronodisruption and social zeitgebers among a population of 15 …

Plio-Pleistocene Antarctic Ice-Ocean Interactions In The Ross Sea, Catherine Prunella

USF Tampa Graduate Theses and Dissertations

Warm, intermediate-depth Southern Ocean waters are implicated in recent Antarctic ice mass loss. Direct observations of Antarctic Ice Sheet (AIS) retreat are temporally limited, necessitating paleoceanographic records of ocean-ice interactions during past warm climate intervals. Deepsea and ice-proximal sediments record orbitally-paced glacial-interglacial fluctuations in AIS volume during the Plio-Pleistocene (last 5 million years; Ma), but the total contribution of the AIS and the role of ocean heat in these fluctuations remain unresolved. To address the response of Antarctica’s ice sheets to changing ocean temperatures during the Plio-Pleistocene, International Ocean Discovery Program (IODP) Expedition 374 recovered sediments from the Ross Sea …

Modeling Early Life: Ontogenetic Growth And Behavior Affect Population Connectivity In Gulf Of Mexico Marine Fish, Kelly Vasbinder

USF Tampa Graduate Theses and Dissertations

This dissertation is an examination of growth, behavior, and dispersal during the early life stages of marine fishes in the Gulf of Mexico. Understanding movements of early life stages is a key part of managing exploited fish populations. Position in the water column can impact larval dispersal, since it determines those currents to which larvae are exposed. First, I investigated the relationship between length and age in early life stages of marine fishes. I found that demersal fish taxa tend to be represented by exponential models, while pelagic fish tend to be represented by linear models. I suggest this may …

Characterizing Childhood And Diet In Migration Period Hungary, Kirsten A. Verostick

USF Tampa Graduate Theses and Dissertations

This project investigates children, childhood and diet of two different Migration Period (4^th-8^th century AD) populations, the Gepids and the Avars, in the Great Hungarian Plain. The main goal was to assess whether there are differences in treatment of children and differences in breastfeeding and weaning practices in these distinct sites and populations. Secondarily, this research also focused on characterizing diet for the Gepids and the Avars at four different sites from the Migration Period, to understand how the migration and settling into the region and the assimilation of other groups into the two populations affected their …

Development Of Next-Generation, Fast, Accurate, Transferable, And Polarizable Force-Fields For Heterogenous Material Simulations, Adam E. Hogan

USF Tampa Graduate Theses and Dissertations

Theoretical modeling is extremely useful in guiding to experiment; however quantitatively accurate modeling of energy-relevant small molecule sorption at the heterogeneous interfaces present in metal-organic materials (MOMs) is currently challenging. MOMs are an emerging class of materials consisting of inorganic clusters and organic linkers that offer great potential in the areas of gas storage, gas separation, and catalysis due to the possibility of large surface areas, complex heterogeneous surfaces, and rational designability. Efficient chemical separations involving these materials could reduce the US’s total energy consumption by approximately 10 to 15%. In this dissertation, the parameterization of small molecules and metal …

Conversion From Metal Oxide To Mof Thin Films As A Platform Of Chemical Sensing, Meng Chen

USF Tampa Graduate Theses and Dissertations

Chemical sensor is working as a widely used device which can be applied to the detection of specific chemicals that are existing in the environment especially in gas phase. The detection of combustible and toxic chemicals can be extremely important in the field of both industrial and civil activities. The chemical sensor is commonly operating by utilizing a chemical or physical interaction between the specific chemical compound and the sensing functional unit, to obtain an electronic signal caused by the property change and realize the chemical detection. Traditional chemical gas sensors such as catalytic gas sensor, thermal conductivity gas sensor, …

Cross-Host Correlations And Multivariate Effects Of Herbivore Specialization, Daniel J. Zydek

USF Tampa Graduate Theses and Dissertations

The distribution of insect herbivores among plant hosts is largely nonrandom: most herbivores have limited sets of hosts within one or a few plant families. This host use specialization is reinforced by traits that confer differential fitness across host plant species. Classic explanations for herbivore specialization predict that evolutionary trade-offs reinforce these relationships by imposing costs in the form of reduced potential fitness on alternative hosts, due to negative genetic correlations in fitness across hosts. This prediction that trade-offs constrain host use in herbivores can be tested with experimental evolution, by showing the direct evolutionary effects of host manipulation on …

An Examination Of An Engagement Indicator For Non-Traditional First-Generation Black Students Enrolled In U.S. Institutions, Chantae D. Still

USF Tampa Graduate Theses and Dissertations

More students than ever have been provided access to higher education; however, the increase in enrollment is outpacing the data on successful completion rates. Student engagement has been identified as fundamental for college student success and retention.

The purpose of this study was to provide a description of the student engagement of non-traditional first-generation Black students attending 4-year public institutions in the United States. Using the Student-Faculty Interaction (SFI) National Survey of Student Engagement (NSSE) scales of student engagement, this study examined the relationship between SFI and selected personal characteristics of traditional and non-traditional, first-generation first-year and senior-year Black students, …

Maternal Social Status, Offspring 2d:4d Ratio And Postnatal Growth, In Macaca Mulatta (Rhesus Macaques), Juan Pablo Arroyo

USF Tampa Graduate Theses and Dissertations

Early life exposure to stressors can disrupt growth and development, resulting in long-term compromised function and increased risk for disease throughout the lifecourse. Maternal exposure to psychosocial stressors (i.e., stressors derived from social status, social inequalities, and social interactions) during pregnancy has been associated with reduced fetal growth, adverse birth outcomes, and increased morbidity for the offspring later in life. Maternal hormonal responses to stress, such as fluctuations in glucocorticoids (e.g., cortisol) and androgens (e.g., testosterone), can result in increased developmental instability, interfere with offspring growth in-utero, and may alter developmental processes of sexual dimorphism. Second digit to fourth digit …

Environmental Influences On Expression Of Virulence- And Survival-Associated Genes And Epigenetic Modifications Of Dna In Vibrio Vulnificus, James W. Conrad

USF Tampa Graduate Theses and Dissertations

Vibrio vulnificus is an autochthonous inhabitant of warm, brackish coastal waters and is an opportunistic pathogen that causes the highest mortality of all seafood-related illnesses (1). These fatal infections are generally caused by the clinically-associated vcgC/16S rRNA type B genotypes within the biotype I group (2–5). However, the reasons for the heightened infectiousness of the clinically-associated strains over environmentally-associated ones remains elusive, as no unique clinically-associated virulence genes have been identified through genomic sequencing or other strategies. DNA methylation may contribute to regulation of virulence by affecting gene transcription, and was investigated in the highly virulent V. vulnificus strain CMCP6. …

Reproduction And Parasite-Mediated Selection, Meredith A. Krause

USF Tampa Graduate Theses and Dissertations

Sexual reproduction generates genetic diversity that can help hosts respond to selection by parasites, and in this thesis, I test three predictions on how reproduction impacts predictions by the parasite theory of sex and the Red Queen hypothesis. In Chapter one, using a meta-analysis, I found that asexuals (lower genetic diversity) have more parasites than sexuals (higher genetic diversity), but this difference can be heavily mediated by the mode and origin of asexuality. Further, hybridization but not polyploidy can blunt predicted differences in parasite loads among sexual and asexual hosts. In Chapter two, I flip the perspective of Red Queen …

Posttranslational Modification And Protein Disorder Regulate Protein-Protein Interactions And Dna Binding Specificity Of P53, Robin Levy

USF Tampa Graduate Theses and Dissertations

p53 is an intrinsically disordered transcription factor that suppresses tumor development by arresting the cell cycle and promoting DNA repair. p53 deletions or mutations can lead to cancer due to the inability of cells to respond to stress. The protein levels and post-translational modification state of p53 changes in response to cellular stress like DNA damage. Previous studies have shown that p53 can undergo coupled folding and binding with the E3 ubiquitin ligase, Mdm2, and the histone deacetylase, p300. In normal cells, p53 is kept at a low level by Mdm2, which marks it with ubiquitin, targeting p53 for proteasome …

Spatial And Temporal Determinants Associated With Eastern Equine Encephalitis Virus Activity In Florida, Kristi M. Miley

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus (EEEV) is considered the most pathogenic mosquito-borne illness in the United States. Human mortality has been estimated to range between 35 to 75%, with horses experiencing mortality rates greater than 90%. A large number of EEEV cases occur in Florida. Though we have come a long way since the first human infection was identified in the 1930s, there is still much to learn regarding the virus’s ability to maintain transmission year-round in Florida. Phylogenic studies support that Florida may serve as the geographic reservoir for EEEV. This research investigated spatiotemporal and ecological variables associated with risk …

Thermoelectric Transport Control Using Single Phase Materials And Metamaterial Composites, Wencong Shi

USF Tampa Graduate Theses and Dissertations

The amount of annual energy consumption in today's human world is on an unprecedented level, as most of the energy used for industrial, transportation, and residential purposes are rejected or dissipated as waste heat, it is of crucial importance to find a way to harvest this wasted heat energy and convert it back into usages. Thermoelectric (TE) devices can help to achieve this purpose by utilizing the principle of thermoelectricity such as the Seebeck effect to generate the electric energy from the waste heat. The main challenges are to find TE materials that are highly efficient in energy conversion, low …

Evaluation Of Aging And Genetic Mutation Variants On Tauopathy, Amber M. Tetlow

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is characterized by amyloid β plaques and neurofibrillary tau tangles (NFTs). While research has demonstrated amyloid pathology occurs prior to tau pathology, or tauopathy, tau has proven to be more toxic. Tauopathy is associated with cognitive declines and neurodegeneration. These findings have highlighted the importance of further understanding tauopathy. In the progression of tauopathy, there is an observable immune response that can be measured by glial cells such as microglia. Activated microglia are known to exacerbate tauopathy rather than reducing the pathology. Research has indicated that with increased age there is an increased risk for AD-related tauopathy …

Discrete Models And Algorithms For Analyzing Dna Rearrangements, Jasper Braun

USF Tampa Graduate Theses and Dissertations

In this work, language and tools are introduced, which model many-to-many mappings that comprise DNA rearrangements in nature. Existing theoretical models and data processing methods depend on the premise that DNA segments in the rearrangement precursor are in a clear one-to-one correspondence with their destinations in the recombined product. However, ambiguities in the rearrangement maps obtained from the ciliate species Oxytricha trifallax violate this assumption demonstrating a necessity for the adaptation of theory and practice.

In order to take into account the ambiguities in the rearrangement maps, generalizations of existing recombination models are proposed. Edges in an ordered graph model …

Porosity And Permeability Extremes In An Eogenetic Carbonate Platform: Mechanisms For Formation And Implications For Fluid Flow, Charles I. Breithaupt

USF Tampa Graduate Theses and Dissertations

Carbonate rocks contain about a third of the worlds drinking water and host 60-70% of proven hydrocarbon reserves. Effective development and management of these resources relies heavily on geologic concepts used to predict the distribution, and magnitude of porosity and permeability in the aquifer or reservoir. Most geologic concepts used for flow prediction have been developed in telegenic limestones, where fracture networks, bedding plains, and conduits hosted in effectively impermeable bedrock control the movement of fluids, and evolution of porosity. However, a growing body of work has recognized fluid flow within eogenetic limestones is fundamentally different, and that new concepts …

“We Developed Solidarity”: Family, Race, Identity, And Space-Time In Recent Multiethnic U.S. American Fiction, Kimber L. Wiggs

USF Tampa Graduate Theses and Dissertations

In Diversity in Families, sociologists Maxine Baca Zinn, D. Stanley Eitzen, and Barbara Wells assert, “At a very personal level, families are crucial shapers of who we are and what our opportunities have been and will be” (xvii). The novels in this dissertation—Octavia Butler’s Kindred (1979), Karen Tei Yamashita’s Tropic of Orange (1997), and Rosaura Sánchez and Beatrice Pita’s Lunar Braceros 2125-2148 (2009)—examine the role of family in the development of individual identity and the practice of social justice. These authors foreground characters from various ethnic backgrounds and depict how the characters form new, multiethnic families. My dissertation explores the …