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New Mechanisms That Control Fact Histone Chaperone And Transcription-Mediated Genome Stability, Angelo Vincenzo De Vivo Diaz

USF Tampa Graduate Theses and Dissertations

The Role of deubiquitinating enzymes (DUBs) in transcription, replication and genome integrity is not one that has been extensively researched. OTU DUBs are a particular class of enzyme with very little known about them.OTUD5 is a cysteine protease in the OTU family responsible to processing lysine 48 and lysine 63 ubiquitin chains. Recently, it has been implicated in to play a role in transcription through its binding partner UBR5. OTUD5 has also been shown to interact with proteins such as PDCD5 and p53, potentially have great importance in cell fate. In this study, I describe new discovered functions for OTUD5 …

Successful Novice Teachers’ Conceptualizations And Enactments Of The Collaborating Teacher Role: A Dual Case Study, Kia Sarnoff

USF Tampa Graduate Theses and Dissertations

The purpose of this descriptive, exploratory, dual case study was to understand the ways two successful novice middle school mathematics teachers serving as first-year collaborating teachers (CTs) for preservice teachers (PSTs) from the same “Grow Your Own” (GYO) educator preparation program (EPP) where they received their initial preparation conceptualized and enacted their CT roles. The CTs’ perceived impact of being alumni of the same middle school mathematics EPP as their PSTs was also explored. Analysis of interview, observation, and document data collected throughout the PSTs’ initial field experience and the CTs’ first-ever mentoring experience resulted in several key assertions including: …

Opto-Fluidic Manipulation Of Microparticles And Related Applications, Hao Wang

USF Tampa Graduate Theses and Dissertations

Contemporary biomedical technologies introduce many new instruments that are important for diagnosis and treatment of the patients. Instrument that can do rapid detection of bacterial infections, and further direct medical practitioner to prescribe optimal antibiotics is highly desirable. But unfortunately, this instrument is not available yet. Ideally, this instrument has to be portable to be used at point-of-care. This means it has to be small and inexpensive, but still highly accurate. Another critical requirement for the instrument is that the test results have to be available promptly. Because in case of such complications of bacterial infections as sepsis, each additional …

Save Water Drink Wine: Challenges Of Implementing The Ethnography Of The Temecula Valley Wine Industry Into Food-Energy-Water Nexus Decision-Making, Zaida E. Darley

USF Tampa Graduate Theses and Dissertations

This study demonstrates the interrelationships of people, food, energy, and water associated with Temecula Valley’s wine industry and reveals contradictions and biases in how people view these resources, which ultimately shape management and policies. The FEW (Food, Energy, and Water) Nexus is an approach increasingly used by policy- and decision-makers to understand the interrelationship of several resources. However, a FEW Nexus approach often lacks in social aspects that influence environmentally and economically sustainable outcomes, especially in the wine and wine tourism industry. When quantitative and qualitative data are available, the other challenge is which assessment to use. Two assessments often …

Coral Reef Restoration In The Tropical West Atlantic Amid The Covid-19 Pandemic, Linden Cheek

USF Tampa Graduate Theses and Dissertations

Climate change is increasing threats to coasts, both from storm surge and sea level rise. Healthy coral reefs provide reduction in storm surges, wave energy, coastal flooding and everyday erosion, and are found across a variety of spatial scales. Given the state of coral reefs worldwide, active Coral Reef Restoration (CRR) in emerging as a necessary component of coastal protection. CRR can be classified as a nature-based solutions (NbS) for coastal protection that also provides a multitude of ecosystem-based services to both humans and other life. Nearly all literature on coral restoration efforts assume a steady-state of human-ecological interactions, but …

A Framework For Assessing The Reliability, Availability, Maintainability, And Safety (Rams) Of Decentralized Sanitation, Adefunké Adeosun

USF Tampa Graduate Theses and Dissertations

In established industry sectors, Reliability, Availability, Maintainability, and Safety (RAMS) risk assessments are normally used to compare the risks associated with different technologies on process units. Sanitation solutions are split into traditional wastewater treatment which is centralized treatment and can be classified as an established industry, and decentralized treatment such as pit latrines and septic tank systems which are on much smaller scales. Also they can be classified as non-conventional methods such as reinvented advanced decentralized non-sewered sanitation solution techniques exist mainly in two forms as “Single-User Reinvented Toilet (SURT)” systems or “Multiple-User Reinvented Toilet (MURT)” systems. Advanced decentralized non-sewered …

The Development Of Aapeptides, Lulu Wei

USF Tampa Graduate Theses and Dissertations

Non-natural peptide mimics are referred to as peptidomimetics, which contains unnatural backbones that mimic the primary peptide structure. There are a large number of peptidomimetics that have been developed including α-peptides, β-peptides, peptoids, arylamide oligomers, cationic polymers, and other peptidomimetics in the last decades. Compared to conventional peptides, these peptidomimetics have many advantages such as resistance to proteolysis, improved bioavailability, and reduced immunogenicity. However, the development of peptidomimetics is still far below the expectation due to the limitation of the availability of backbones. It is an urgent need to generate a new diverse library for novel drug discovery, design antimicrobial …

Analytical Methods And Critical Analyses Supporting Thermodynamically Consistent Characterizations Of The Marine Co2 System, Jonathan D. Sharp

USF Tampa Graduate Theses and Dissertations

Chemical equilibria describing the unique behavior of gaseous and ionic forms of dissolved carbon dioxide (CO₂) in seawater comprise what is known as the marine CO₂ (or carbonate) system. Observations of the marine CO₂ system with high degrees of accuracy, reproducibility, spatial coverage, and temporal resolution are critical for evaluating natural cycles of carbon within the Earth system, as well as chemical and biological responses to anthropogenic CO₂ emissions.

One component of the CO₂ system is the carbonate ion (CO²−₃), a dissolved ion that is produced when carbonic acid (H …

Fostering Success Through Coaching: Perspectives Of Help Seeking Within A Coaching Relationship With Post-Secondary Students From Foster Care, Jamie L. Bennett

USF Tampa Graduate Theses and Dissertations

Coaching, a humanistic approach to growth, has recently been utilized on college campuses as an alternative to standard student service strategies to address the needs of students from foster care. For this dissertation, I have collected interview data from four coaches and five college students who had spent time in the foster care system to explore the perceptions of help-seeking behaviors and coaching within a campus-based university program. The goal was to understand the role of coaching in the students’ help-seeking behaviors. The theories of self-determination and survivalist self-reliance were used to conceptualize my literature review, research design, and data …

Feasting On Words: What University Students Learn When They Study Food Writing And Food Media, Janet K. Keeler

USF Tampa Graduate Theses and Dissertations

The use of food in college curriculum is unique in its ability to create lasting impact because of the keen interest millennial and Generation Z students have in what they eat and drink. Studying media with food at its core is an underutilized mechanism to show how food intersects with the lives of all people thus encouraging students to look beyond their own experiences to consider the wider society. A program evaluation of 10 semesters of food writing and food media courses at a Florida public university reveals the ways in which students make deeper connections to culture and current …

Transformational School Leadership And Students' Gaokao Performance In China: A Multilevel Mediation Analysis, Yanzheng Li

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine how teachers’ psychological contract fulfillment (PCF) and organizational commitment (OC) mediated the effects of principals’ transformational school leadership (TSL) on students’ Gaokao performance in China by testing a hypothesized multilevel model. Survey data were collected online from 893 teachers at 10 public senior high schools in a city located in Northern China. The first city-wide mock Gaokao exam results were used as the indicator of students’ Gaokao performance. Data analysis techniques included correlational analysis, multiple regression analysis, confirmatory factor analysis, structural equation modeling analysis, and multilevel mediation analysis. Results indicated a relatively …

The Effect Of Hope On The Relationship Between Personal And Disease Characteristics And Anxiety And Depression In Adolescents And Young Adults With Cancer, Sharon B. Mcneil

USF Tampa Graduate Theses and Dissertations

Adolescents and young adults (AYAs) with cancer face physical, psychosocial, emotional, and developmental challenges that are unique to their age group. In facing the crisis of a cancer diagnosis, both social support and hope have been shown to be tools utilized by some AYAs. However, AYAs have higher rates of anxiety and depression than in older adults which may have an effect on their levels of hope.

This cross-sectional study examined the mechanistic effects of hope on the relationship between personal characteristics of age, gender, and social support, the disease characteristics of type of cancer and length of time since …

Multifunctional Metal-Organic Frameworks (Mofs) For Applications In Sustainability, Gaurav Verma

USF Tampa Graduate Theses and Dissertations

Climate change, affordable and clean energy, and responsible consumption and production are some of the important goals to be achieved for sustainable development. The current technologies used to address these challenges are energy and resource intensive. Furthermore, there are various limitations related to cost, storage, scalability and safety.Adsorbent materials are seen as a promising alternative remediation system due to their low energy consumption and minimal chemical waste production. However, most of the traditional adsorbents, such as activated carbon and zeolites lack the structural tunability to have long-term effectiveness and their use is limited. As discussed in Chapter 1, metal organic-frameworks …

Origins Of Amyloid Oligomers And Novel Approaches For Their Detection, Jeremy Barton

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease, type II diabetes, and other amyloid diseases are known to be associated with the formation of amyloid aggregates. It has been thoroughly researched whether amyloid fibrils or oligomers are the main culprit for these diseases, and recent evidence has connected oligomers as the most disease relevant aggregate species. However, many difficulties have arose in confirming this hypothesis. Techniques for oligomer detection are often limited in their sensitivity, and in many cases are unable to distinguish oligomers from rigid fibrils. Additionally, the role oligomer splay in fibril assembly is still unclear, and has led to the belief that different …

The Use Of Spanish Moss As A Biological Indicator To Examine Relationships Between Metal Air Pollution, Vegetation Cover, And Environmental Equity In Tampa, Florida, Yousif Abdullah

USF Tampa Graduate Theses and Dissertations

Studies of inequality in exposure to less common air pollutants, like metals, are often limited by the costs of high spatial resolution measurements. Spanish moss (Tillandsia usneoides) is a promising bioindicator for measuring air pollution due to its lower cost, enabling capture of time-average environmental concentrations at high spatial resolution. This study had three major aims. First, I aimed to use Spanish moss as a bioindicator to characterize ambient concentrations of selected metals (Ti, Cr, Mn, Co, Ni, Cd, Hg, Pb, As, and Sb) in Tampa, Florida. My second goal was to determine the impact of vegetation cover on metals …

Chinese International Students’ Narratives Of Their Experiences In A Graduate Pathway Program: An Exploratory Study, Lu Norstrand

USF Tampa Graduate Theses and Dissertations

Because of the tremendous growth of Chinese international students’ enrollment in American higher education and the rapid rise and development of Pathway programs in American universities, I determined the main purpose of this research was to determine the experiences of three Chinese international students in a Graduate Pathway program at a public four-year university in the Southeastern U.S. Three research questions were addressed:

1. How do three Chinese international students describe their academic experiences in a Graduate Pathway program in the U.S.?
2. How do these students describe differences in their learning experiences between the U.S. and China?
3. How do these students …

Assessing Many-Body Van Der Waals Contributions In Model Sorption Environments, Matthew K. Mostrom

USF Tampa Graduate Theses and Dissertations

The energy used in the separation and purification of small volatile atoms and compounds represents a sizablefraction of the world’s energy consumption as a whole 1 Noble gases in particular represent a unique challenge as their separation currently relies on distillation methods that work by virtue of small differences in the density of their pure phases. Highly porous Metal-Organic Materials (MOMs) offer a cheaper, more efficient alternative to cryogenic distillation, and studying their properties using various simulation methods has become an important and active area of research. The family of Potentials with “High Accuracy, Speed and Transferability” (PHAST) have been …

Applying The Situational Theory Of Publics To Children's Sex Education In China, Baoyi Zeng

USF Tampa Graduate Theses and Dissertations

In China, "sex" is a sensitive, even taboo topic. In this special cultural background, the lack of sex education for children is worthy of attention. This study aims to extend the Situational Theory of Public (STP) to the field of sex education for children. The results of an online questionnaire survey among 424 Chinese parents show that a high degree of problem recognition and high level of participation in child sex education will lead Chinese parents to actively search for relevant information on this issue. Being able to identify children's sex education problems, having a low barrier to solving the …

Succession In Native Groundwater Microbial Communities In Response To Effluent Wastewater, Chelsea M. Dinon

USF Tampa Graduate Theses and Dissertations

Aquifer storage and recovery techniques are used globally to mitigate increasing demands for groundwater. Microcosms were used to evaluate the effect of wastewater effluent that may be used for aquifer storage and recovery injection on native aquifer microbial communities. Sulfur Springs water was used as the source of aquifer water. The microcosms were 100% spring water, 100% unchlorinated filtered wastewater effluent, or one of three mixtures of unchlorinated filtered wastewater effluent and spring water. The mixture microcosms were 50%, 30%, and 10% unchlorinated filtered wastewater effluent with the balance as spring water. The effluent water was UV treated prior to …

Identifying The Academic And Emotional Risk And Resource Factors Of Underrepresented Students In Accelerated Coursework, Jasmine L. Gray

USF Tampa Graduate Theses and Dissertations

There are many benefits of student participation in accelerated curricula in high school such as the International Baccalaureate Diploma Program (IBD) or Advanced Placement (AP) coursework. Benefits include skipping introductory coursework in college, being better prepared to deal with the stressors of college, and positive impacts on peer relationships, self-image, and the development of the concept of success (The International Baccalaureate Organization [IBO], 2019). However, the extent to which all students, regardless of demographic background, are able to participate in and benefit from such programs is a little less known. Previous research indicates that the IBD tends to enroll high-achieving …

Honokiol Loaded Mpeg-Pcl Micelles For The Treatment Of Age-Related Macular Degeneration, Amna Shahid

USF Tampa Graduate Theses and Dissertations

Age related Macular Degeneration (AMD), a multi-factorial age-related retinal hypoxic disorder resulting in irreversible loss of vision is the foremost cause of blindness in United States. Current treatment strategies used to treat disease involve multiple intraocular injections of Anti – VEGF agents into the vitreous of eye. In addition to the issues of drug localization and targeted delivery, the need to frequently inject drug into the eye raise patient compliance issues. These challenges call for sustained drug delivery system. In this study, a sustained drug delivery system was prepared by loading an anti- HIF agent, honokiol into MPEG-PCL polymer. These …

Hepatobiliary Polycyclic Aromatic Hydrocarbons In Pelagic Fishes Of The Gulf Of Mexico, Madison R. Schwaab

USF Tampa Graduate Theses and Dissertations

Fisheries populations and their ecosystems are negatively impacted by both chronic and acute inputs of pollutants, including oil spills such as the Deepwater Horizon platform blowout in 2010 in the Gulf of Mexico (GoM). After Deepwater Horizon, toxicological studies of demersal fishes of the northern GoM were undertaken to characterize impacts and to establish baseline contaminant levels in the aftermath of the spill. In this study, I quantify polycyclic aromatic hydrocarbon (PAH) concentrations in eight pelagic fish species to demonstrate oil exposure differences between species, region and time. Analysis of biliary PAH metabolite equivalents using high performance liquid chromatography was …

Convergence Of Auditory Nerve Fibers Onto Globular Bushy Cells, Matthew Kersting

USF Tampa Graduate Theses and Dissertations

Globular bushy cells (GBCs) of the cochlear nucleus are specialized neurons that encode the temporal features of sound. Multiple auditory nerve inputs are known to synapse onto a single GBC, but the exact number and sizes of these inputs have not been systematically investigated in adult mice. To gain a high-resolution and unbiased look at the auditory inputs contacting GBCs, our lab utilized Serial Block-Face Scanning Electron Microscopy. Specifically, 21 GBCs and all their large inputs were reconstructed at nanometer resolution. To produce the most precise results, we applied careful attention to the reconstruction and implemented cutting-edge meshing algorithms. We …

Why Are Women Leaving Stem? An Examination Of Workplace Rivalry, Joseph Regina

USF Tampa Graduate Theses and Dissertations

Past research on workplace rivalry has framed the construct as a motivating force that is desirable for individuals and organizations. Using social comparison theory as a framework, the potential harmful correlates of rivalry were examined. Specifically, the relationships between the status of having a rival, as well as one’s perception of their relative standing to (i.e. being better or worse than) their rival, and the outcomes of turnover intention, perceived competence, and imposter syndrome were analyzed. Further, to examine how STEM fields may be particularly impacted by these relationships, both job and STEM turnover were measured, and the moderating effect …

Immune Modulation As A Therapeutic Target In An Α-Synuclein Model Of Parkinson’S Disease, Meena Subhashini Subbarayan

USF Tampa Graduate Theses and Dissertations

Parkinson’s disease (PD) is the second most common neurodegenerative disorder affecting about 1.5 million people in the United States with more than 60,000 people diagnosed each year. It is classically characterized by four major symptoms: tremor, postural instability, stiffness in joints, and slow movement (bradykinesia). Pathologically PD is characterized by up to 70% loss of dopaminergic neurons in substantia nigra pars compacta (SNpc) of midbrain and accumulation of presynaptic protein called α-synuclein (α-syn) within dopaminergic neurons that extend to the striatum. This disrupts the nigrostriatal pathway leading to the motor symptoms seen in PD patients. Microglia, the innate immune cells …

Bodily Harm: An Analysis Of The Phenomenological And Linguistic Aspects Of Harm And Trauma, Grant Samuel Peeler

USF Tampa Graduate Theses and Dissertations

This work seeks to explore the phenomenological experience of harm through an investigation of trauma and its existential features. Harm, despite its importance for many topics in both Political Science and Political Theory, is not often investigated as a subject in itself. By interrogating elements of Merleau-Ponty’s uniquely embodied philosophy, this work seeks to further our understanding of harm as a phenomenon which is both uniquely subjective and yet socially informed.

The text is split into two halves – with the first offering an exegesis of relevant sections of Merleau-Ponty’s Phenomenology of Perception, and the second engaging with contemporary secondary …

New Mechanisms That Regulate Dna Double-Strand Break-Induced Gene Silencing And Genome Integrity, Dante Francis Deascanis

USF Tampa Graduate Theses and Dissertations

Proliferating cells are constantly threatened by genotoxic stressors that can potentially lead to genomic instability. Breaks in the DNA, namely double-strand breaks, are detrimental sources of damage that must be repaired to maintain genomic integrity and prevent potential tumorigenesis. Here we discuss a gene silencing mechanism flanking damaged chromatin. Gene silencing and transcriptional repression at damaged DNA are necessary to prevent potential genomic aberrations from occurring through conflicts with the DNA repair machinery. BMI1, a core polycomb protein in the polycomb repressive complex 1 (PRC1) has been known to play a role in gene silencing at damaged chromatin. However, the …

Optimal Strategies For Grassland Restoration To Increase Spatial Carrying Capacity Of Florida Sandhill Cranes In Pasco County, Florida, Ibrahim Kaya

USF Tampa Graduate Theses and Dissertations

The Florida sandhill crane (Antigone canadensis pratensis) is a non-migratory bird species occurring throughout the Florida peninsula and the Okefenokee Swamp in southeastern Georgia. Human land-altering activities have led to a substantial decline in the amount and condition of suitable habitat, including grasslands, for the species in Florida, thereby decreasing the population size significantly. Grasslands located in close proximity to nesting locations are one of the habitat types on which the population viability of the species depends, as they provide foraging and brooding habitat which is crucial for successful reproduction. The purpose of this study is to propose the most …

Regulation Of The Heat Shock Response And Hsf-1 Nuclear Stress Bodies In C. Elegans, Andrew Deonarine

USF Tampa Graduate Theses and Dissertations

The Heat Shock Response (HSR) is a highly conserved stress responsive molecular pathway that functions to promote appropriate protein folding in the cell. The HSR accomplishes this primarily through the use of molecular chaperones that serve to bind to misfolded or unfolded proteins to assist in stabilizing and folding proteins back to their native functional state. The master regulator of this pathway is a transcription factor known as Heat Shock Factor 1 (HSF1). HSF1 regulates molecular chaperone expression in the cell’s basal state, but can also be stress induced by diverse biotic and abiotic signals including thermal shock, oxidative stress, …

Reproductive Ecology And Phenology Of Thalassia Testudinum (Hydrocharitaceae) In Tampa Bay, Florida, Sheila Scolaro

USF Tampa Graduate Theses and Dissertations

Successful sexual reproduction and recolonization are critical in maintaining genetic diversity within seagrass meadows. Thalassia testudinum flower, fruit and seedling production were assessed visually at 10 sites within Tampa Bay, Florida approximately every 4 weeks from May to July 2017 to determine if there is spatial and temporal variation in sexual reproductive effort and if location within a meadow effects sexual reproductive effort. Results from this study reveal strong temporal variability throughout the reproductive season. The month of May was observed as peak Thalassia testudinum anthesis and June was peak fruiting in Tampa Bay during 2017. Flower and fruit density …