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Investigating The Novel Role Of Dextromethorphan In Mitigating Oxidative Stress Induced Changes In Retinal Pigment Epithelial Cells (Rpe), Zeinab A. Mohamed

USF Tampa Graduate Theses and Dissertations

Oxidative stress contributes to the degeneration of retinal pigment epithelial (RPE) cells and photoreceptors that lead to the dry form of age-related macular degeneration. Activation of N-Methyl-D-aspartate (NMDA) receptors contributes to the increase of free radicals that induce oxidative stress-induced changes. Our study investigates if Dextromethorphan, an NMDA receptor antagonist, reverses oxidative stress-induced changes in RPE. We used Paraquat and H2O2 as oxidative stress-inducing agents to induce oxidative damage in human ARPE-19 cells. To illustrate the change in cell viability and measure the apoptosis of ARPE-19 cells from oxidative damage, we performed MTT assay and Annexin V-DAPI flow cytometry analysis. …

Warming Up And Cooling Down: Perceptions And Behaviors Associated With Aerobic Exercise, Balea J. Schumacher

USF Tampa Graduate Theses and Dissertations

This study investigated the perceptual and behavioral responses regarding the use of warm-ups and cool-downs in aerobically active adults. There were 79 individuals who volunteered to participate in this study. Respondents were recruited by word of mouth or through social media and the Qualtrics survey link/QR code was on all recruitment materials in order to access the survey. The survey consisted of 47 questions. This study aimed to determine whether age, sex, or exercise location impacted perception or behavior related to warm-up and/or cool- down activities before or after the aerobic training exercise sessions. Primary results indicated the following: 1) …

The Perceived Utility Of Direct-To-Consumer Genetic Testing Marketed For Athletic Ability, Katherine L. Zimmerman

USF Tampa Graduate Theses and Dissertations

Factors that influence the public’s interest in pursuing genetic testing to determine athletic ability are not yet known. The purpose of this study is to compare interest in genetic testing marketed for athletic ability and susceptibility to sports-related injuries among athletes and the general public. Additionally, this study aims to determine if genetic determinism, knowledge and elements of perceived utility influence their overall intention to use the results of such genetic testing. Participants were recruited through social media platforms to complete an online survey measuring their intention to pursue testing under various circumstances, intention to use the results to change …

Passive Nitrifying Biofilters For Onsite Treatment Of Saline Domestic Wastewater, Daniel Arnulfo Delgado

USF Tampa Graduate Theses and Dissertations

The infrastructure to support the growing world population has polluted many water bodies with nitrogen from urban and agricultural runoff and failing wastewater systems, such as improperly sited and maintained septic systems. Septic systems present a low energy and maintenance option to treat domestic wastewater and is a treatment option that can be done far from a centralized grid. This further reduces cost, maintenance, and energy as it reduces the need to build and maintaining sewage piping and the infrastructure to move the water longer distances. However, these systems tend to fail when their upkeep is left to homeowners that …

A Comparative Study Of Male And Female Undergraduate Computer Science Students’ Educational Pathways, Stephanie Fitzsimmons

USF Tampa Graduate Theses and Dissertations

Science, Technology, Engineering and Mathematics (STEM), including Computer Science (CS) are fields that are in great demand globally. This study’s purpose was to explore the nature of the educational pathways, critical factors and commonalities/differences leading to CS undergraduate enrollment through the male and female perspectives focusing on personal/home, academic/attitude and psychological factors underlying the Social Cognitive Career Theory factors. Purposive sampling method was used for this multi-case study, comprised of CS undergraduate upperclassman. Participants shared their perspectives on their CS educational pathway via three interviews and journals. Thematic analysis of narrative for both individual and cumulative group analysis, plus researcher …

Bait-And-Kill: Targeting A Novel Heme Biochemical Pathway In Hundreds Of Cancers, Christopher G. Marinescu

USF Tampa Graduate Theses and Dissertations

Cancer cells are characterized by their ability to grow and proliferate in rates that far exceed normal cells by modifying their iron/heme metabolisms to levels higher than normal. This imbalance of heme biosynthesis can lead to cancer cells having a flux of heme intermediates to the point where they enter a state called heme-overdrive. Heme-overdrive is a process unique to a variety of cancers but absent in normal tissues. With this enhanced production, heme can act as an epigenic regulator for signaling proliferation (18). Through the novel strategy called ‘bait-and-kill,’ cancer cells will be coerced into a state of heme …

Americans’ Familiarity, Interest, And Actions With Direct-To-Consumer Genetic Testing, Riley L. Carroll

USF Tampa Graduate Theses and Dissertations

Background: In 2013, 36% of the U.S. public was aware of Direct-to-Consumer (DTC) genetic tests, but updated studies are needed to identify whether this has changed over time. Objective: The aims are to determine current awareness and utilization of DTC tests and to identify demographic factors that influence testing uptake. Methods: An online panel survey of 543 U.S. adults was conducted using age- and sex-based quotas. Descriptive statistics and binary regression identified factors associated with undergoing DTC testing and linear regression was conducted to identify factors associated with likelihood of using DTC testing for athletic ability to …

The Little Things That Count: Mapping Diversity Of West Florida’S Shelf, Nicole L. Seiden

USF Tampa Graduate Theses and Dissertations

Biodiversity is essential to the ecosystem functions responsible for generating the multi-billion-dollar industries that the US relies on for food, storm mitigation, tourism and recreation, but is facing increasing natural and anthropogenic threats. High-resolution diversity data covering large spatial areas would enhance current ecology knowledge and better address conservation concerns in the Gulf of Mexico. However, acquiring and handling data of this nature is resource intensive and has not yet been established. In this study, high spatial resolution data was collected from gastropod living and death assemblages collected over 10 years from the West Florida Shelf (WFS). A multivariate regression …

Drug Candidates Targeting Hsp90/Cdc37 For The Treatment Of Neurodegenerative Disease, Ryan Martin

USF Tampa Graduate Theses and Dissertations

Neurodegenerative disease refers to the death of specific populations of neurons that canresult in dementia, loss of voluntary muscle motility, and other abnormalities. Two major neurodegenerative diseases are Alzheimer’s disease (AD) and Parkinson’s disease (PD), which are linked to abnormalities in Tau and a-synuclein proteins, respectively. Currently, there is no cure for these diseases. Our group and others have shown that chaperone proteins play a critical role in the processing of Tau and a-synuclein. The inhibition of the Hsp90/Cdc37 chaperone complex is shown to affect the levels of these two proteins. Hence, we hypothesized that a drug molecule, conglobatin (Cb), …

Acclimatization Protocols And Their Outcomes, Ayub M. Odera

USF Tampa Graduate Theses and Dissertations

Heat acclimatization provides the opportunity to better tolerate heat stress. Different methods are used to acclimatize participants as part of heat stress studies in the laboratory. The usual acclimatization protocols are greater than the occupational exposure limit represented by the ACGIH TLV® and NIOSH REL. The purpose of this paper was to examine the acclimatization state prior to and at the end of a one-week acclimatization program using two acclimatization protocols.

Prior heat stress studies at the University of South Florida (USF) used two heat stress conditions for participants’ acclimatization. Participants (n = 43) were evaluated using four different studies …

Nest Site Choice As A Potential Climate Response In The Gopher Tortoise (Gopherus Polyphemus), A Threatened Species, Meghan Craft

USF Tampa Graduate Theses and Dissertations

Climate warming is expected to continue in coming decades, posing a threat to species worldwide. While some species may be able to shift their ranges in the pursuit of cooler temperatures, a large portion of species may have to find ways to adapt to warmer conditions in situ. Oviparous reptiles may be able to buffer against rising temperatures by altering nest site choice. In this study, I quantified nest site choice and its thermal consequences in a population of the gopher tortoise (Gopherus polyphemus) in southwestern Florida. I found marked variation in the openness of selected nest sites, …

Tourism, Education, And Identity Making: Agency And Representation Of Indigenous Communities In Public Sites Within Florida., Timothy R. Lomberk Ii

USF Tampa Graduate Theses and Dissertations

National parks have a history of complex relations with Native communities beginning with the advent of the National Park Service to representation of Native histories in the present. I will focus on two specific cases located within Florida, utilizing the lens of authorized heritage to show challenges of representation; and the range of ways that representation has been addressed and are currently being addressed with respect to Native communities in Florida. I utilize ethnohistorical and ethnographic methods to explore issues of representation in public spaces; such as museums and national parks. I am interested in critical representations of Native American …

Quantifying The Gametogenic Cycle And Reproductive Efforts In The Eastern Oyster (Crassostrea Virginica) In Florida Estuaries, Nicole Patrice Maloney

USF Tampa Graduate Theses and Dissertations

Oyster populations from six estuaries in Florida were sampled monthly from January to December of 2018 for reproduction and health analysis. Sites were located in Apalachicola Bay, Caloosahatchee River estuary, Lake Worth lagoon, Loxahatchee River estuary, St. Lucie estuary, and Tampa Bay. Oysters monitored for analysis in South Florida (Loxahatchee River estuary, St. Lucie estuary, and Caloosahatchee River estuary) were chosen as they are a part of a monitoring component of the Comprehensive Everglades Restoration Plan. These estuaries are highly influenced by freshwater input from Lake Okeechobee and other river basins. Like the South Florida sites Apalachicola Bay is also …

¿De Dónde Eres?: Negotiating Identity As Third Culture Kids, Sophia Margulies

USF Tampa Graduate Theses and Dissertations

Sparked by David C. Pollock's (1988) concept of "third culture kids" (children of government officials and military personnel), this thesis uses autoethnography to examine "white-passing" Latinx identity and gender passing as it relates to individuals who identify as transgender to understand what it means to "pass" within these communication contexts. I situate the study at the intersections of queer, trans, and Latinx theories. Ultimately, I argue that the communicative and identity practices inherent to the liminal spaces in which third culture kids perform create the conditions for performances as transnational subjects. What the contexts of place and home are like, …

Glutathione-S-Transferase Mu 1 (Gstm1) Gene Augmentation Attenuated Oxidative Stress Induced Changes In Retinal Pigment Epithelium Cells (Rpe), Riddhi Sandeep Vichare

USF Tampa Graduate Theses and Dissertations

Purpose: Loss of Retinal Pigment Epithelial (RPE) cells and photoreceptors leads to Age-related macular degeneration (AMD), one of the leading causes of blindness in the elderly worldwide. Oxidative stress-mediated damage is one of the factors that impair the survival of RPE and photoreceptors in dry-AMD. We sought to determine the role of human Glutathione S-Transferase Mu-1 (GSTM1) in protecting RPE cells from oxidative stress-induced changes.

Method: We cloned human GSTM1 cDNA with P2A tag into a lenti-plasmid vector and later made a lentivector to express GSTM1. After that, we infected human retinal pigment epithelial cells (ARPE-19), screened stable ARPE-19 cells …

The Distribution In Native Populations From Mexico And Central America Of The C677t Variant In The Mthfr Gene, Lucio A. Reyes

USF Tampa Graduate Theses and Dissertations

Objectives: To explore evolutionary hypotheses for the high frequencies of a substitution in the methylenetetrahydrofolate reductase (MTHFR) gene, in Mexican and Central American Indigenous populations.

Materials and methods: We obtained allele frequencies for the C677T variant in the MTHFR gene and ecological information for 37 indigenous samples from Mexico and Central America. We calculated Hardy–Weinberg equilibrium and computed Fst statistics. We computed correlations between the samples' allele frequencies and ecological and geochemical variables.

Results: Many of the samples have extremely high frequencies of the T allele (q̄ = 0.62, median = 0.66). In this region, the frequency of the T …

Fabrication And Characterization Methods Of Self-Cleaning Coatings For Solar Panels Application, Mohammed Khaleel M Alhussain

USF Tampa Graduate Theses and Dissertations

Transparent self-cleaning coatings with superhydrophobic and superhydrophilic behavior are of great interest in a wide range of industrial applications such as automotive, solar panels, windows, and optical devices. Each has its uses, properties, and characteristics. For the use of these coatings on solar panels, certain features are important in maintaining the efficiency of the panels in different weather conditions and reducing the operation and maintenance cost, such as self-cleaning, high transparency, antifouling, anti-fogging, and anti-icing. Herein, we have investigated the fabrication of self-cleaning coatings using two simple methods and ways to improve their characteristics. In the first method, a hydrophobic …

Effect Of Temperature On Growth And Gene Expression In The Marine Diatom, Thalassiosira Pseudonana, John M. Foster

USF Tampa Graduate Theses and Dissertations

Diatoms are unicellular, eukaryotic, photosynthetic algae responsible for the production of 20% of our atmospheric oxygen. Diatoms contribute to several important biogeochemical functions. Diatoms contribute to the carbon cycle, sequestering carbon and forming oxygen as a product of photosynthesis. Moreover, by converting abiotic forms of energy such as sunlight into organic compounds (i.e., sugars, starches and lipids), primary producers including diatoms, plants, and phytoplankton feed organisms at higher trophic levels. Consequently, changes in temperature, light intensity, nutrients, salinity and other stress factors that affect primary producers can generate a potentially catastrophic ripple effect at higher trophic levels In this study …

Shorebird Response To Human-Induced Changes At Three Pinellas County Beaches, Rebecca J. Ruthberg-Campagna

USF Tampa Graduate Theses and Dissertations

Worldwide, shorebird habitat is being destroyed and degraded by development and sea level rise. Shorebirds depend on availability of pristine, undisturbed coastal habitats for resting and feeding during migration as well as for reproduction. Migratory shorebirds using the East Atlantic Flyway visit the Gulf of Mexico Beaches of Pinellas County, Florida as a stopover site during Fall and Spring migration. In addition to hosting migratory species, Pinellas County beaches are home to several year-round resident species that breed during Summer. Pinellas County is the most densely populated county in Florida, and its Gulf Coast is heavily developed with commercial and …

Eating And Body Image Disorders In The Time Of Covid19: An Anthropological Inquiry Into The Pandemic’S Effects On The Bodies, Theresa A. Stoddard

USF Tampa Graduate Theses and Dissertations

This thesis examines how the COVID-19 pandemic and its associated lifestyle changes are impacting the experiences of self-identifying women and females with body image disorders (BIDs) and/or eating disorders (EDs), focusing on the mental, physical, and emotional health of participants. Using surveys, person-centered semi-structured interviews, and autoethnography, I collected qualitative and quantitative data regarding the challenges, triumphs, hopes, and fears of participants regarding their EDs/BIDs during the pandemic and situated their experiences within their sociocultural context. Drawing on anthropological and psychological theory, I examine the data through the lenses of Scheper-Hughes’s and Lock’s “The Three Bodies” (the body politic, body …

Our Story, Our Homeland, Our Legacy: Settlement Patterns Of The Geechee At Sapelo Island Georgia, From 1860 To 1950, Colette D. Witcher

USF Tampa Graduate Theses and Dissertations

The Gullah Geechee are descendants of enslaved Africans who worked on the island coastal plantations of Georgia, North and South Carolina, and Northern Florida. The Gullah Geechee are known for having retained African cultural connections and practices in the United States through intergenerational knowledge transmission. On Sapelo Island, Georgia, during their 250 years on the island a least 15 historic communities have been acknowledged as representative of their distinct presence on the island. Hog Hammock which was established in 1878, is the last intact Gullah Geechee owned community, due to forced abandonment of the other communities during the early 1950s. …

The Impact Of Bahiagrass (Paspalum Notatum) On Florida Sand Skink (Plestiodon Reynoldsi) Movements, Chandler A. R. Eaglestone

USF Tampa Graduate Theses and Dissertations

The Florida Sand Skink (Plestiodon reynoldsi) is a federally threatened lizard precinctive to scrub habitat, a pyrogenic habitat that experiences fires infrequently. Plestiodon reynoldsi spends most of its time right under the sand surface, moving, or “sand swimming,” through the granular substrate found in Florida’s central ridges. While the distribution of this species has been greatly reduced, agricultural development of P. reynoldsi habitat has led to potentially suitable fragmented scrub occurring near bahiagrass (Paspalum notatum) pastures. Bahiagrass is widely used as a pasture grass in sandy soils of the southeast United States, because of its deep …

Novel Co2 Transporters From Autotrophic Bacteria From Extreme Environments, Sarah E. Schmid

USF Tampa Graduate Theses and Dissertations

Autotrophic organisms are responsible for introducing carbon into food webs. These organisms take up dissolved inorganic carbon (DIC) and use it as their major carbon source, converting it to biomass using a variety of carbon fixing pathways (e.g., the Calvin-Benson-Bassham cycle). In nature, DIC concentrations can be low or temporally heterogeneous, so autotrophic organisms adapt with CO2 concentrating mechanisms (CCMs) to facilitate the acquisition and fixation of this dissolved gas. CCMs consist of membrane-spanning DIC transporters, which elevate intracellular DIC concentrations, which in turn are harvested by carboxysomes, proteinaceous microcompartments that contain the carboxylase Ribulose-1,5-bisphosphate carboxylase-oxygenase (RubisCO) and carbonic anhydrase. …

Plasmonic Photocatalysis For Gas-Phase Degradation Of Total Volatile Organic Compounds: Theory, Experimentation, And Catalyst Stability, Amaury P. Betancourt Iii

USF Tampa Graduate Theses and Dissertations

Plasmonic nanomaterials have become a strong contender for improving the efficiency of photocatalytic degradation of air pollutants. This study demonstrates an easy and scalable fabrication method, employing electron beam evaporation and rapid thermal annealing, for producing plasmonic photocatalysts. Samples were made by either coating silver on, or layering silica-protected (i.e., silica-coated) or unprotected (i.e., uncoated) silver beneath, the photocatalyst (either zinc oxide or titanium dioxide). Stability and catalytic performance for gas-phase toluene degradation was assessed by monitoring total volatile organic compound (TVOC) concentration versus ultraviolet-A (UV-A) illumination time in a recirculating batch reactor (plate-type flow-through). Samples were characterized using a …

The General Psychopathology Factor (P) From Adolescence To Adulthood: Disentangling The Developmental Trajectories Of P Using A Multi-Method Approach, Alexandria M. Choate

USF Tampa Graduate Theses and Dissertations

Considerable attention is directed towards studying co-occurring psychopathology through the lens of a general factor (p-factor). However, the developmental trajectories and stability of the p-factor have yet to be fully understood. Study 1 first examined the explanatory power of dynamic mutualism theory — an alternative framework positing the p-factor to be a product of lower-level symptom interactions rather than the inherent cause of them. Predictions of dynamic mutualism were tested using three distinct statistical approaches including: longitudinal bifactor models, random-intercept cross-lagged panel models (RI-CLPMs), and network models. Next, given prior suggestions that borderline personality disorder (BPD) could be a marker …

The Relationship Between New Graduate Registered Nurse Knowledge, Experiences, Attitudes, And Age Bias Toward The Older Adult, Michelle Fox

USF Tampa Graduate Theses and Dissertations

There had been a lack of research on the relationship between a new graduate Registered Nurses’ knowledge, experiences, attitudes, and age bias toward older adults. The purpose of this study was to investigate whether there was a relationship between new graduate Registered Nurses’ knowledge, experiences, attitudes, and age bias toward older adults.

The researcher used correlational, non-experimental, quantitative design for this study. The instruments used in this study were the Facts on Aging Quiz, the Kogan Attitude Toward Old People scale, and a demographic questionnaire. Descriptive statistics were used to analyze the demographic data and correlation coefficients were used to …

Does Better A1c Control Worsen Osteoarthritis? An Electronic Health Record Cross-Sectional Study, Sarah C. Cattaneo

USF Tampa Graduate Theses and Dissertations

Obesity is a major risk factor for osteoarthritis (OA). There is evidence that diabetes also increases risk. Our hypothesis is that A1C is a predictor of OA severity. The aim is to investigate the association between A1C, BMI, and knee and hip OA severity. This is a cross-sectional study within the Veterans Health Administration (VHA) database containing 818 patients with diagnosed diabetes. Patients at one VHA facility with recorded diabetes in fiscal year 2020 were identified. A1C and BMI data was obtained from the electronic health record. Chart reviews were performed to collect data on imaging reports of weight-bearing joints …

Synthesizing Laccol Based Polymers/Copolymers And Polyurethanes; Characterization And Their Applications, Imalka Marasinghe Arachchilage

USF Tampa Graduate Theses and Dissertations

In modern world research scopes are highly favored the areas of developing new polymer materials which are more integrated with the environment. Synthesizing eco-friendly, bio degradable polymer materials to replace petroleum based synthetic polymers to utilize in radiation hard applications is one of the main objectives of this study. 'Laccol' extracted from Vietnamese lacquer tree (Toxicodendron succedanea) polymerized with other monomers namely styrene and d-limonene (from citrus waste) respectively to develop copolymers via cationic polymerization. Laccol contains hydroxyl phenyl groups that act as radical scavengers, enhancing radiation resistance via their ability to convert excitation energy into non-chemistry inducing energy in …

Saudi Teachers’ Perceptions Of Rough-And-Tumble Play In Early Learning, Rana Alghamdi

USF Tampa Graduate Theses and Dissertations

This study explored teachers’ perceptions of rough-and- tumble (R&T) play in early childhood education in Saudi Arabia. The literature on rough-and-tumble play in Saudi Arabia is limited in scope, and more research is needed to explore teachers’ perceptions on this type of play for early learners. The pertinent literature reveals that R&T play, which includes running, jumping, fighting, wrestling, chasing, pulling, pushing, and climbing, among other rough playful activities, can positively impact learning and development across psychosocial, emotional, and cognitive domains. Teachers’ understanding of R & T play is key, and the attitudes of Saudi early childhood teachers who are …

Plastic Pollution In Urban Rivers: Spatial And Temporal Patterns Of Plastic Release And Transport, Charlotte Juliane Haberstroh

USF Tampa Graduate Theses and Dissertations

This dissertation investigates plastic release and transport in urban rivers with a focus on the driving spatial and temporal factors. Plastic is a key pollutant of this century that affects humans and the environment around the world. Rivers are a focal point of release and management, as they concentrate and accumulate plastics from urban watersheds and convey them to the world’s oceans.

This research assessed cross-sectional, longitudinal, and seasonal patterns of plastics in rivers, and evaluated the role of urban pollution and watershed hydrology. A detailed background is provided in the literature review. This work is built on two case …