Calibration Of A Common Shortwave Multispectral Camera System For Quantitative Agricultural Applications,
2020
University of Nebraska-Lincoln
Calibration Of A Common Shortwave Multispectral Camera System For Quantitative Agricultural Applications, J. Burdette Barker, Wayne Woldt, Brian Wardlow, Christopher Michael Usher Neale, Mitchell S. Maguire, Bryan Leavitt, Derek M. Heeren
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Unmanned aerial systems (UAS) for collecting multispectral imagery of agricultural fields are becoming more affordable and accessible. However, there is need to validate calibration of sensors on these systems when using them for quantitative analyses such as evapotranspiration, and other modeling for agricultural applications. The results of laboratory testing of a MicaSense (Seattle, WA, USA) RedEdge™ 3 multispectral camera and MicaSense Downwelling Light Sensor (irradiance sensor) system using a calibrated integrating sphere were presented. Responses of the camera and irradiance sensor were linear over many light levels and became non-linear at light levels below expected real-world, field conditions. Simple linear …
High-Throughput Screening Of Clinically Approved Drugs That Prime Nonviral Gene Delivery To Human Mesenchymal Stem Cells,
2020
University of Nebraska-Lincoln
High-Throughput Screening Of Clinically Approved Drugs That Prime Nonviral Gene Delivery To Human Mesenchymal Stem Cells, Tyler Kozisek, Andrew Hamann, Albert Nguyen, Michael Miller, Sarah Plautz, Angela K. Pannier
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Background: Human mesenchymal stem cells (hMSCs) are intensely researched for applications in cell therapeutics due to their unique properties, however, intrinsic therapeutic properties of hMSCs could be enhanced by genetic modification. Viral transduction is efficient, but suffers from safety issues. Conversely, nonviral gene delivery, while safer compared to viral, suffers from inefficiency and cytotoxicity, especially in hMSCs. To address the shortcomings of nonviral gene delivery to hMSCs, our lab has previously demonstrated that pharmacological ‘priming’ of hMSCs with the glucocorticoid dexamethasone can significantly increase transfection in hMSCs by modulating transfection-induced cytotoxicity. This work seeks to establish a library of transfection …
Deep Learning Predicts Microbial Interactions From Self-Organized Spatiotemporal Patterns,
2020
Pacific Northwest National Laboratory
Deep Learning Predicts Microbial Interactions From Self-Organized Spatiotemporal Patterns, Joon-Yong Lee, Natalie C. Sadler, Robert G. Egbert, Christopher R. Anderton, Kirsten S. Hofmockel, Janet K. Jansson, Hyun-Seob Song
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Microbial communities organize into spatial patterns that are largely governed by interspecies interactions. This phenomenon is an important metric for understanding community functional dynamics, yet the use of spatial patterns for predicting microbial interactions is currently lacking. Here we propose supervised deep learning as a new tool for network inference. An agent-based model was used to simulate the spatiotemporal evolution of two interacting organisms under diverse growth and interaction scenarios, the data of which was subsequently used to train deep neural networks. For small-size domains (100 mm x 100 mm) over which interaction coefficients are assumed to be invariant, we …
Swine Slurry Characteristics As Affected By Selected Additives And Disinfectants,
2020
University of Nebraska - Lincoln
Swine Slurry Characteristics As Affected By Selected Additives And Disinfectants, Jon Duerschner, Shannon L. Bartelt-Hunt, Kent Eskridge, John E. Gilley, Xu Li, Amy M. Schmidt, Daniel D. Snow
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Current swine industry practice is to house animals in confinement facilities which capture and store feces and urine as slurry in pits below the production area. Additives and disinfectants may be introduced into the manure pits. This study was conducted to measure the effects of additives and disinfectants on temporal changes in swine slurry characteristics. Slurry from a commercial swine production facility in southeast Nebraska, USA was collected and transferred to 57 L reactors located within a greenhouse. Selected additives and disinfectants were added to the reactors and physical properties, chemical characteristics, and antibiotic concentrations were monitored for 40 days. …
Influence Of Setback Distance On Antibiotics And Antibiotic Resistance Genes In Runoff And Soil Following The Land Application Of Swine Manure Slurry,
2020
University of Nebraska - Lincoln
Influence Of Setback Distance On Antibiotics And Antibiotic Resistance Genes In Runoff And Soil Following The Land Application Of Swine Manure Slurry, Maria C. Hall, Noelle Mware, John E. Gilley, Shannon L. Bartelt-Hunt, Daniel D. Snow, Amy M. Schmidt Schmidt, Kent M. Eskridge, Xu Li
Department of Agricultural and Biological Systems Engineering: Faculty Publications
The environmental spread of antibiotics and antibiotic resistance genes (ARGs) from the land application of livestock wastes can be a potential public health threat. The objective of this study was to assess the effects of setback distance, which determines how close manure may be applied in relation to surface water, on the transport of antibiotics and ARGs in runoff and soil following land application of swine manure slurry. Rainfall simulation tests were conducted on field plots covered with wheat residues, each of which contained an upslope manure region where slurry was applied and an adjacent downslope setback region that did …
Geostatistical Features Of Streambed Vertical Hydraulic Conductivities In Frenchman Creek Watershed In
Western Nebraska,
2020
University of Nebraska - Lincoln
Geostatistical Features Of Streambed Vertical Hydraulic Conductivities In Frenchman Creek Watershed In Western Nebraska, Olufemi P. Abimbola, Aaron R. Mittelstet, Troy E. Gilmore
Department of Agricultural and Biological Systems Engineering: Faculty Publications
This study evaluated the spatial variability of streambed vertical hydraulic conductivity (Kv) in different stream morphologies in the Frenchman Creek Watershed, Western Nebraska, using different variogram models. Streambed Kv values were determined in situ using permeameter tests at 10 sites in Frenchman, Stinking Water and Spring Creeks during the dry season at baseflow conditions. Measurements were taken both in straight and meandering stream channels during a 5 day period at similar flow conditions. Each test site comprised of at least three transects and each transect comprised of at least three Kv measurements. Linear, Gaussian, exponential …
Evaluation Of Low-Cost Depth Cameras For Agricultural Applications,
2020
“Luiz de Queiroz” College of Agriculture & University of Nebraska-Lincoln & University of Illinois at Urbana Champaign
Evaluation Of Low-Cost Depth Cameras For Agricultural Applications, Isabella C.F.S. Condotta, T. M. Brown-Brandl, Santosh Pitla, John P. Stinn, Késia O. Silva-Miranda
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Low-cost depth-cameras have been used in many agricultural applications with reported advantages of low cost, reliability and speed of measurement. However, some problems were also reported and seem to be technology- related, so understanding the limitations of each type of depth camera technology could provide a basis for technology selection and the development of research involving its use. The cameras use one or a combination of two of the three available technologies: structured light, time-of-flight (ToF), and stereoscopy. The objectives were to evaluate these different technologies for depth sensing, including measuring accuracy and repeatability of distance data and measurements at …
Classification Of Tactile And Motor Velocity-Evoked Hemodynamic Response In Primary Somatosensory And Motor Cortices As Measured By Functional Near-Infrared Spectroscopy,
2020
University of Nebraska - Lincoln
Classification Of Tactile And Motor Velocity-Evoked Hemodynamic Response In Primary Somatosensory And Motor Cortices As Measured By Functional Near-Infrared Spectroscopy, Mohsen Hozan, Jacob Greenwood, Michaela Sullivan, Steven M. Barlow
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Functional near-infrared spectroscopy (fNIRS) is an emerging technique in studying cerebral hemodynamics; however, consensus on the analysis methods and the clinical applications has yet to be established. In this study, we demonstrate the results of a pilot fNIRS study of cerebral hemodynamic response (HR) evoked by pneumotactile and sensorimotor stimuli on the dominant hand. Our goal is to find the optimal stimulus parameters to maximally evoke HR in the primary somatosensory and motor cortices. We use a pulsatile pneumatic array of 14 tactile cells that were attached to the glabrous surface of the dominant hand, with a patterned stimulus that …
Glucocorticoid Priming Of Nonviral Gene Delivery To Hmscs Increases Transfection By Reducing Induced Stresses,
2020
University of Nebraska - Lincoln
Glucocorticoid Priming Of Nonviral Gene Delivery To Hmscs Increases Transfection By Reducing Induced Stresses, Andrew Hamann, Tyler Kozisek, Kelly Broad, Angela K. Pannier
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Human mesenchymal stem cells (hMSCs) are under study for cell and gene therapeutics because of their immunomodulatory and regenerative properties. Safe and efficient gene delivery could increase hMSC clinical potential by enabling expression of transgenes for control over factor production, behavior, and differentiation. Viral delivery is efficient but suffers from safety issues, while nonviral methods are safe but highly inefficient, especially in hMSCs. We previously demonstrated that priming cells with glucocorticoids (Gcs) before delivery of DNA complexes significantly increases hMSC transfection, which correlates with a rescue of transfection-induced metabolic and protein synthesis decline, and apoptosis. In this work, we show …
Ultrasmall Mixed Eu−Gd Oxide Nanoparticles For Multimodal
Fluorescence And Magnetic Resonance Imaging Of Passive
Accumulation And Retention In Tbi,
2020
University of Nebraska - Lincoln
Ultrasmall Mixed Eu−Gd Oxide Nanoparticles For Multimodal Fluorescence And Magnetic Resonance Imaging Of Passive Accumulation And Retention In Tbi, Badrul Alam Bony, Hunter A. Miller, Aria W. Tarudji, Connor C. Gee, Anandakumar Sarella, Michael G. Nichols, Forrest Kievit
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. TBI can have a long-term impact on the quality of life for survivors of all ages. However, there remains no approved treatment that improves outcomes following TBI, which is partially due to poor delivery of therapies into the brain. Therefore, there is a significant unmet need to develop more effective delivery strategies that increase the accumulation and retention of potentially efficacious treatments in the injured brain. Recent work has revealed that nanoparticles (NPs) may offer a promising approach for site-specific delivery; however, a detailed understanding of the …
Robotic Detection And Grasp Of Maize And Sorghum:
Stem Measurement With Contact,
2020
University of Nebraska-Lincoln
Robotic Detection And Grasp Of Maize And Sorghum: Stem Measurement With Contact, Abbas Atefi, Yufeng Ge, James C. Schnable
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Frequent measurements of the plant phenotypes make it possible to monitor plant status during the growing season. Stem diameter is an important proxy for overall plant biomass and health. However, the manual measurement of stem diameter in plants is time consuming, error prone, and laborious. The use of agricultural robots to automatically collect plant phenotypic data for trait measurements can overcome many of the drawbacks of manual phenotyping. The objective of this research was to develop a robotic system that can automatically detect and grasp the stem, and measure its diameter of maize and sorghum plants. The robotic system comprises …
Rapeseed Stand Count Estimation At Leaf Development Stages With Uav Imagery And Convolutional Neural Networks,
2020
Huazhong Agricultural University, Wuhan & Ministry of Agriculture, Wuhan
Rapeseed Stand Count Estimation At Leaf Development Stages With Uav Imagery And Convolutional Neural Networks, Biquan Zhao, Chenghai Yang, Yeyin Shi, Qingxi Liao, Guangsheng Zhou, Chufeng Wang, Tianjin Xie, Zhao Jiang, Dongyan Zhang, Wanneng Yang, Chenglong Huang, Jing Xie, Jian Zhang
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Rapeseed is an important oil crop in China. Timely estimation of rapeseed stand count at early growth stages provides useful information for precision fertilization, irrigation, and yield prediction. Based on the nature of rapeseed, the number of tillering leaves is strongly related to its growth stages. However, no field study has been reported on estimating rapeseed stand count by the number of leaves recognized with convolutional neural networks (CNNs) in unmanned aerial vehicle (UAV) imagery. The objectives of this study were to provide a case for rapeseed stand counting with reference to the existing knowledge of the number of leaves …
Optimal Depth Jump Height Quantified As Percentage Of Athlete Stature,
2020
University of Nebraska - Lincoln
Optimal Depth Jump Height Quantified As Percentage Of Athlete Stature, Curtis L. Tomasevicz, Ryan Hasenkamp, Jack W. Ransone, David Jones
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Purpose: An individual’s optimal depth jump platform height provides a resistive force which allows an athlete to rebound with substantial velocity resulting in maximum power exertion. The objective of this investigation was to show that the optimal platform height in a depth jump can be quantified as a percentage of individual body stature which can serve as measurable quantified value. Although athlete height is not highly correlated to power ability nor does a universal height exist, this value can provide a basis for a rehabilitation or strength and conditioning program. The desired intensity of a program can be prescribed as …
Comparison Of Object Detection And Patch-Based Classification Deep Learning Models On Mid- To Late-Season Weed Detection In Uav Imagery,
2020
University of Nebraska - Lincoln
Comparison Of Object Detection And Patch-Based Classification Deep Learning Models On Mid- To Late-Season Weed Detection In Uav Imagery, Arun Narenthiran Veeranampalayam Sivakumar, Jiating Li, Stephen Scott, Eric T. Psota, Amit J. Jhala, Joe D. Luck, Yeyin Shi
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Mid- to late-season weeds that escape from the routine early-season weed management threaten agricultural production by creating a large number of seeds for several future growing seasons. Rapid and accurate detection of weed patches in field is the first step of site-specific weed management. In this study, object detection-based convolutional neural network models were trained and evaluated over low-altitude unmanned aerial vehicle (UAV) imagery for mid- to late-season weed detection in soybean fields. The performance of two object detection models, Faster RCNN and the Single Shot Detector (SSD), were evaluated and compared in terms of weed detection performance using mean …
Temporal Trend Analysis Of Meteorological Variables And Reference Evapotranspiration In The Inter-Mountain Region Ofwyoming,
2020
University of Florida
Temporal Trend Analysis Of Meteorological Variables And Reference Evapotranspiration In The Inter-Mountain Region Ofwyoming, Vivek Sharma, Christopher Nicholson, Antony Bergantino, Suat Irmak, Dannele Peck
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Long-term trends in reference evapotranspiration (ETref) and its controlling factors are critical pieces of information in understanding how agricultural water requirements and water resources respond to a variable and changing climate. In this study, ETref, along with climate variables that directly and indirectly impact it, such as air temperature (T), incoming solar radiation (Rs), wind speed (u), relative humidity (RH), and precipitation (P), are discussed. All variables are analyzed for four weather stations located in irrigated agricultural regions of inter-mountain Wyoming: Pinedale, Torrington, Powell, andWorland. Non-parametric MannKendall (MK) trend test and Theil–Sen’s slope estimator were used to determine the statistical …
Classification Of Tactile And Motor Velocity-Evoked Hemodynamic Response In Primary Somatosensory And Motor Cortices As Measured By Functional Near-Infrared Spectroscopy,
2020
University of Nebraska - Lincoln
Classification Of Tactile And Motor Velocity-Evoked Hemodynamic Response In Primary Somatosensory And Motor Cortices As Measured By Functional Near-Infrared Spectroscopy
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Functional near-infrared spectroscopy (fNIRS) is an emerging technique in studying cerebral hemodynamics; however, consensus on the analysis methods and the clinical applications has yet to be established. In this study, we demonstrate the results of a pilot fNIRS study of cerebral hemodynamic response (HR) evoked by pneumotactile and sensorimotor stimuli on the dominant hand. Our goal is to find the optimal stimulus parameters to maximally evoke HR in the primary somatosensory and motor cortices. We use a pulsatile pneumatic array of 14 tactile cells that were attached to the glabrous surface of the dominant hand, with a patterned stimulus that …
Review—Single Walled Carbon Nanotubes As Optical Sensors For Biological Applications,
2020
University of Nebraska - Lincoln
Review—Single Walled Carbon Nanotubes As Optical Sensors For Biological Applications, Eric M. Hofferber, Joseph A. Stapleton, Nicole M. Iverson
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Since the discovery of the band gap fluorescence from single walled carbon nanotubes (SWNT) many advancements have been made towards the use of these unique fluorophores as optical biosensors in vitro, ex vivo in vivo. Attention has been given to these pure carbon structures due to their photostability, tunable properties, and bright near infrared emission that falls in the tissue transparency window. This review highlights some of the major advancements in the field of SWNT biosensors over the last two decades with a focus given to recent advances in biological applications.
Predicting Total Petroleum Hydrocarbons In Field Soils With
Vis–Nir Models Developed On Laboratory-Constructed
Samples,
2020
University of Nebraska - Lincoln
Predicting Total Petroleum Hydrocarbons In Field Soils With Vis–Nir Models Developed On Laboratory-Constructed Samples, Nuwan Wijewardane, Post Doc Research Associate, Yufeng Ge, Natasha Sihota, Thomas Hoelen, Toni Miao, David C. Weindorf
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Accurate quantification of petroleum hydrocarbons (PHCs) is required for opti- mizing remedial efforts at oil spill sites. While evaluating total petroleum hydro- carbons (TPH) in soils is often conducted using costly and time-consuming lab- oratory methods, visible and near-infrared reflectance spectroscopy (Vis–NIR) has been proven to be a rapid and cost-effective field-based method for soil TPH quantification. This study investigated whether Vis–NIR models calibrated from laboratory-constructed PHC soil samples could be used to accurately estimate TPH concentration of field samples. To evaluate this, a laboratory sample set was constructed by mixing crude oil with uncontaminated soil samples, and two field …
Representing Organic Matter Thermodynamics In Biogeochemical Reations Via Substrate-Explicit Modeling,
2020
Pacific Northwest National Laboratory, Richland & University of Nebraska - Lincoln
Representing Organic Matter Thermodynamics In Biogeochemical Reations Via Substrate-Explicit Modeling, Hyun-Seob Song, James C. Stegen, Emily B. Graham, Joon-Yong Lee, Vanessa A. Garayburu-Caruso, William C. Nelson, Xingyuan Chen, J. David Moulton, Timothy D. Scheibe
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Predictive biogeochemical modeling requires data-model integration that enables explicit representation of the sophisticated roles of microbial processes that transform substrates. Data from high-resolution organic matter (OM) characterization are increasingly available and can serve as a critical resource for this purpose, but their incorporation into biogeochemical models is often prohibited due to an over-simplified description of reaction networks. To fill this gap, we proposed a new concept of biogeochemical modeling—termed substrate-explicit modeling—that enables parameterizing OM-specific oxidative degradation pathways and reaction rates based on the thermodynamic properties of OM pools. Based on previous developments in the literature, we characterized the resulting kinetic …
Within-Field Variability In Granular Matrix Sensor Data And Its Implications For Irrigation Scheduling,
2020
University of Nebraska-Lincoln
Within-Field Variability In Granular Matrix Sensor Data And Its Implications For Irrigation Scheduling, T. Lo, H.C. Pringle Iii, Daran Rudnick, Geng Bai, L. Jason Krutz, D.M. Gholson, Xin Qiao
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Even when located within the same field, multiple units of the same soil moisture sensor rarely report identical values. Such within-field variability in soil moisture sensor data is caused by natural and manmade spatial heterogeneity and by inconsistencies in sensor construction and installation. To better describe this variability, daily soil water tension values from 14-23 sets of granular matrix sensors during the middle part of four soybean site-years in the Mississippi Delta were analyzed. The soil water tension data were found to follow approximately normal distributions, to exhibit moderately high temporal rank stability, and to show strong positive correlation between …
