Environmental Engineering Commons^™

Comparative Study Of Human Pluripotent Stem Cell-Derived Endothelial Cells In Hydrogel-Based Culture Systems, Zhanqi Wang, Fuxing Zuo, Qing Liu, Xuesheng Wu, Qian Du, Yuguo Lei, Zhangmin Wu, Haishuang Lin

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Human pluripotent stem cell (hPSC)-derived endothelial cells (ECs) are promising cell sources for drug discovery, tissue engineering, and studying or treating vascular diseases. However, hPSC-ECs derived from different culture methods display different phenotypes. Herein, we made a detailed comparative study of hPSC-ECs from three different culture systems (e.g., 2D, 3D PNIPAAm-PEG hydrogel, and 3D alginate hydrogel cultures) based on our previous reports. We expanded hPSCs and differentiated them into ECs in three culture systems. Both 3D hydrogel systems could mimic an in vivo physiologically relevant microenvironment to protect cells from shear force and prevent cell agglomeration, leading to a high …

Adaptive Laboratory Evolution Of Scenedesmus Obliquus For Increased Carbohydrate Content And Biomass Productivity, Nahel Ali

Master's Theses

The economics of microalgal bioproduct commercialization would benefit from increased accumulation of energy storage compounds, such as carbohydrates and lipids, and increased biomass productivity. This thesis explores two adaptive laboratory evolution strategies for improving Scenedesmus obliquus: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection under outdoor conditions in a raceway pond at a high dilution rate to increase biomass productivity.

Two cultigens were generated with the goal of increased carbohydrate content: K5 and K7. Both were mutagenized for 5 seconds and cultivated in 50-mL …

Re-Aerosolization Of Dense Metal Oxide Simulating Radiological Contamination From Military Clothing, Joseph M. Chapman

Theses and Dissertations

Radiological contamination from nuclear accidents or the terrorist use of a Radiological Dispersal Device (RDD) are events that first responders must be prepared for. It is currently assumed that removal of the victims clothing will remove 80-90 of contamination. Many RDD radioisotopes produce dense aerosols that would then contaminate victim clothing and re-aerosolize during clothing removal. A series of experiments were conducted during which clothing was contaminated with a dense aerosol simulating the radioisotope of Strontium 90. Compared to background, there was statistically significant re-aerosolization from the clothing. This demonstrates inhalation hazards from re-aerosolization of RDD contamination.

An Analysis Of Various Hydrogen Production Methods For Fleet Vehicle Fuel, Eugene J. Denezza Ii

Theses and Dissertations

As conflict, the environment, and politics cause changes around the world, the United States Air Force is pursuing a diversification of drop-in and alternative fuel sources. Hydrogen fuel cells for vehicles are gaining popularity worldwide. Yet, how to best produce hydrogen gas still remains a question. This thesis will provide an overview of various hydrogen production methods and their respective environmental impacts, costs, efficiencies, and viability; and will perform sensitivity analysis to determine an optimal solution. The optimal production method is Thermolysis followed by Steam Reformation of Landfill or Natural Gas, Coal Gasification, and PEM Electrolysis. Based on varying requirements …

The Effect Of Molar Peroxide Ratio On The Oxidation Of Bisphenol A In An Ultraviolet Light Emitting Diode/Hydrogen Peroxide Advanced Oxidation Process, Megan C. Shade

Theses and Dissertations

Bisphenol A is a compound widely used in industry that is detrimental to human health and ecological systems. Ultraviolet light emitting diodes and hydrogen peroxide can be combined t produces hydroxyl radicals. These highly reactive radicals have the potential to degrade contaminants in water. This research utilized 50, 100, 250, 500, and 1000:1 H₂O₂:BPA molar ratios for analysis. These results illustrated that the reactions at the 50, 100, and 250:1 ratios were hydrogenperoxide limited. The 1000:1 results exhibited evidence of radical scavenging that limited the degradation of BPA.

The Effect Of Molar Peroxide Ratio On The Oxidation Of 2,4-Dinitroanisole In An Ultraviolet Light Emitting Diode Advanced Oxidation Process, Troy M. Searcy

Theses and Dissertations

Insensitive munitions (IMs) are increasingly employed by the U.S. military to prevent unintended detonations associated with conventional munitions. One IM used extensively is 2,4 dinitroanisole (DNAN). This research employed an ultraviolet light emitting diode (LED) and hydrogen peroxide advanced oxidation process (AOP) to treat DNAN-contaminated water at the laboratory scale. Five molar peroxide ratios (50:1, 100:1, 250:1, 500:1, 1000:1) were evaluated using the observed pseudo first-order rate constant as the performance metric. Both peroxide-limiting reactions and radical scavenging were observed over the range of ratios tested, with overall removal of DNAN decreasing above a ratio of 500:1.

Evaluation Of Efficient Water Reuse Technologies For Sustainable Forward Operating Bases, Kelsie L. Crouch

Theses and Dissertations

A secure water source is essential to the resiliency and readiness of military installations and contingency operation locations, especially those located in the dry climates the US military operates in today. There are multiple issues of concern when identifying water sources, such as security, cleanliness, accessibility, and sustainability. Water resources pose a potential vulnerability to mission readiness in a remote, agile environment, such as a forward operating base (FOB). Having a secure water resource helps facilitate mission readiness in the contingency environment, offering the opportunity to be more resilient and cost-effective. Current water treatment technologies present the possibility to perform …

Fluid Dynamics Modeling For Per- And Polyfluoroalkyl Substances Removal In Legacy Fire Suppression Systems, Jacob G. Spaulding

Theses and Dissertations

Legacy per- and polyfluoroalkyl substances (PFASs) exist in the form of aqueous film-forming foams within hangar fire suppression systems throughout the country, posing a threat to both surrounding environments as well as local populations. The United States (US) Department of Defense (DoD), in conjunction with the US Environmental Protection Agency (EPA), is investigating best practices to flush out existing contaminants prior to disposal and replacement with a less environmentally harmful replacement. While current procedures dictate a triple rinse of the entire system to yield contaminant levels acceptable to the DoD, the practice has never truly been tested. Designed as a …

Determination Of Vadose Zone And Saturated Zone Nitrate Lag Times Using Long-Term Groundwater Monitoring Data And Statistical Machine Learning, Martin J. Wells, Troy E. Gilmore, Natalie Nelson, John K. Bohlke

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In this study, we explored the use of statistical machine learning and long-term groundwater nitrate monitoring data to estimate vadose zone and saturated zone lag times in an irrigated alluvial agricultural setting. Unlike most previous statistical machine learning studies that sought to predict groundwater nitrate concentrations within aquifers, the focus of this study was to leverage available groundwater nitrate concentrations and other environmental variables to determine mean regional vertical velocities (transport rates) of water and solutes in the vadose zone and saturated zone (3.50 and 3.75 m yr−1 , respectively). The statistical machine learning results are consistent with two primary …

How A Low-Cost Method For Cumulative Water-Sampling Shows Need For Improvement Of Legal Public-Contact Standards In The United States, Samuel C. Kessler

Grawemeyer Colloquium Papers

Across the world, it is estimated that 4.5 billion people live near water sources “impaired” for use or contact. Standards for human-interaction are established by international organizations such as the WHO, and legislative bodies from national to local levels with jurisdiction over the quality of our waterways to ensure public & environmental health. Standards are often assessed from “grab-samples” taken from a waterbody at a certain time, with a minimum number analyzed. Water-quality standards in the United States are enforced under the Clean Water Act (CWA) via the Environmental Protection Agency (EPA), applying to “waters of the United States” (WOTUS). …

Role Of Nadþ In Regulating Cellular And Metabolic Signaling Pathways, Sara Amjad, Sabah Nisar, Ajaz A. Bhat, Ab Rauf Shah, Michael P. Frenneaux, Khalid Fakhro, Mohammad Haris, Ravinder Reddy, Zoltan Patay, Joseph Baur, Puneet Bagga

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Nicotinamide adenine dinucleotide (NAD⁺), a critical coenzyme present in every living cell, is involved in a myriad of metabolic processes associated with cellular bioenergetics. For this reason, NAD⁺ is often studied in the context of aging, cancer, and neurodegenerative and metabolic disorders.

Scope of review: Cellular NAD⁺ depletion is associated with compromised adaptive cellular stress responses, impaired neuronal plasticity, impaired DNA repair, and cellular senescence. Increasing evidence has shown the efficacy of boosting NAD⁺ levels using NAD⁺ precursors in various diseases. This review provides a comprehensive understanding into the role of NAD^{+ …}

Historical Contingency In Microbial Resilience To Hydrologic Perturbations, Hyun-Seob Song, James C. Stegen, Emily B. Graham, Timothy D. Scheibe

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Development of reliable biogeochemical models requires a mechanistic consideration of microbial interactions with hydrology. Microbial response to and its recovery after hydrologic perturbations (i.e., resilience) is a critical component to understand in this regard, but generally difficult to predict because the impacts of future events can be dependent on the history of perturbations (i.e., historical contingency). Fundamental issues underlying this phenomenon include how microbial resilience to hydrologic perturbations is influenced by historical contingency and how their relationships vary depending on the characteristics of microbial functions. To answer these questions, we considered a simple microbial community composed of two species that …

Modeling The Release And Spreading Of Permanganate From Aerated Slow-Release Oxidants In A Laboratory Flow Tank, Ann Kambhu, Yusong Li, Troy E. Gilmore, Steve D. Comfort

School of Natural Resources: Faculty Publications

Aerated, slow-release oxidants are a relatively new technology for treating contaminated aquifers. A critical need for advancing this technology is developing a reliable method for predicting the radius of influence (ROI) around each drive point. In this work, we report a series of laboratory flow tank experiments and numerical modeling efforts designed to predict the release and spreading of permanganate from aerated oxidant candles (oxidant-wax composites). To mimic the design of the oxidant delivery system used in the field, a double screen was used in a series of flow tank experiments where the oxidant was placed inside the inner screen …

Improving Vertical Axis Wind Turbine (Vawt) Performance With Placement Strategies: Final Report, Patrick A. Tebbe, Nazli A. Yilmaz Wodzinski, Namyong Lee

Mechanical and Civil Engineering Department Publications

This project was focused on the use and placement of vertical axis wind turbines (VAWTs). Studies were focused on areas not typically addressed in previous work; small wind at very low elevations (i.e. within the boundary layer). Numerical studies were made of how VAWTs function as well as the potential wind environments they may be placed in. A simplified numerical code, based on two-dimensional potential flow functions, was developed to model flow around buildings and other structures. The code allows a rapid determination of regions where flow may experience speed-up and where turbulence may result.

Experimental facilities were designed and …

A Life Cycle Comparison Of Remote, Deployed Expeditionary Waste Management Scenarios, David J. Chester, Chandrani Mukherjee, Jeremy M. Slagley, Eric Mbonimpa, Thomas Hornstein

Faculty Publications

The current barrier to acquisition and utilization of viable waste-to-energy (WTE) technologies at remote or deployed expeditionary sites requires high capital and operation & maintenance costs. The impacts to environment and human health of differing expeditionary waste management strategies were compared using the Life Cycle Assessment software SimaPro 8.0. Emissions of individual waste management scenarios were compiled from peer-reviewed literature, converted to values compatible with SimaPro’s waste scenario inputs, and the calculated impacts compared using SimaPro’s pre-loaded methodologies. These calculated impacts and the economic impacts confirm that open-air burning of waste is not only dangerous to humans and the environment, …

2021 Final Butte Reduction Works (Brw) Phase I Quality Assurance Project Plan (Qapp) Revision 3. February 2021, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Predicting The Effect Of Fly Ash On Concrete’S Mechanical Properties By Ann, Mohammad Mehdi Roshani, Seyed Hamidreza Kargar, Visar Farhangi, Moses Karakouzian

Civil and Environmental Engineering and Construction Faculty Research

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. Fly ash, as a supplemental pozzolanic material, reduces concrete’s adverse environmental footprint by decreasing the emission of carbon dioxide (CO2 ) during the cement manufacturing process. Fly ash, which is a waste material, can enhance both the mechanical characteristics and durability of concrete, and has the capability to play an important role in sustainable design. Considering the widespread interest in applying Fly ash, and despite research studies, the level of replacement is still unclear. In this paper, a novel method using artificial neural networks (ANN) is presented to predict concrete’s mechanical …

Modeling The Effectiveness Of Cooling Trenches For Stormwater Temperature Mitigation, Scott A. Wells

Civil and Environmental Engineering Faculty Publications and Presentations

Due to elevated runoff stormwater temperatures from impervious areas, one management strategy to reduce stormwater temperature is the use of underground flow through rock media termed a cooling trench. This paper examines the governing equations for the liquid phase and media phases for modeling the temperature leaving a cooling trench assuming that changes in temperature occurred longitudinally through the cooling trench. This model is dependent on parameters such as the media type, porosity, media initial temperature, inflow rate, and inflow temperature. Several approaches were explored mathematically for evaluating the change in temperature of the water and the cooling trench media. …

Covid-19: A Spectio-Temporal Analysis Of Air Quality Status In Coking Coalfields Of India Due To Nationwide Lockdown In India, Amarjeet Singh Mr., Gurdeep Singh Dr.

Journal of Sustainable Mining

A novel infectious corona virus disease (COVID-19) was identified in month of December 2019.It has now been announced as a worldwide pandemic by the World Health Organization [1]. The COVID-19 pandemic has positive impacts on the environmental pollutants. In present work, Coalfield areas of Jharia Coalfields (JCF), India have been taken as a case study to evaluate the effect of lockdown on air quality at 10 locations. This study had been selected to estimate the reduction in concentration of pollutants like PM₁₀, PM_2.5, SO₂, and NOx during 3 Seasons (summer, Post-Monsoon and winter season) …

New Particle Formation In The Remote Marine Boundary Layer, Guangjie Zheng, Yang Wang, For Full List Of Authors, See Publisher's Website.

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Marine low clouds play an important role in the climate system, and their properties are sensitive to cloud condensation nuclei concentrations. While new particle formation represents a major source of cloud condensation nuclei globally, the prevailing view is that new particle formation rarely occurs in remote marine boundary layer over open oceans. Here we present evidence of the regular and frequent occurrence of new particle formation in the upper part of remote marine boundary layer following cold front passages. The new particle formation is facilitated by a combination of efficient removal of existing particles by precipitation, cold air temperatures, vertical …