Environmental Engineering Commons^™

Temporal Endogenous Gene Expression Profiles In Response To Lipid-Mediated Transfection, Timothy M. Martin, Sarah A. Plautz, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background — Design of efficient nonviral gene delivery systems is limited as a result of the rudimentary understanding of the specific molecules and processes that facilitate DNA transfer.

Methods — Lipoplexes formed with Lipofectamine 2000 (LF2000) and plasmid-encoding green fluorescent protein (GFP) were delivered to the HEK 293T cell line. After treating cells with lipoplexes, HG-U133 Affymetrix microarrays were used to identify endogenous genes differentially expressed between treated and untreated cells (2 h exposure) or between flow-separated transfected cells (GFP+) and treated, untransfected cells (GFP–) at 8, 16 and 24 h after lipoplex treatment. Cell priming studies were conducted using …

Robust And Flexible Films From 100% Starch Cross-Linked By Biobased Disaccharide Derivative, Helan Xu, Hazal Canisag, Bingnan Mu, Yiqi Yang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In this research, oxidized sucrose, a novel aldehyde-based green cross-linker, endowed starch films with substantial improvement in both tensile strength and elongation, whereas many other cross-linkers did not. Starch films are usually weak, brittle, and highly moisture sensitive, and thus have restricted industrial applications. Cross-linking is one of the most common methods to tackle these problems. However, most of the available cross-linkers are either toxic, expensive, or with low cross-linking efficiencies. Oxidized sucrose is a green cross-linker with multiple aldehyde groups per molecule to cross-link starch molecules via forming hemiacetals/acetals. The starch films cross-linked with oxidized sucrose had tensile strength …

Bionanotechnology And The Future Of Glioma, Peter A. Chiarelli, Forrest M. Kievit, Miqin Zhang, Richard G. Ellenbogen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Designer nanoscaled materials have the potential to revolutionize diagnosis and treatment for glioma. This review summarizes current progress in nanoparticle‑based therapies for glioma treatment including targeting, drug delivery, gene delivery, and direct tumor ablation. Preclinical and current human clinical trials are discussed. Although progress in the field has been significant over the past decade, many successful strategies demonstrated in the laboratory have yet to be implemented in human clinical trials. Looking forward, we provide examples of combined treatment strategies, which harness the potential for nanoparticles to interact with their biochemical environment, and simultaneously with externally applied photons or magnetic fields. …

Integrating Ecological And Engineering Concepts Of Resilience In Microbial Communities, Hyun-Seob Song, Ryan S. Renslow, Jim K. Fredrickson, Stephen R. Lindemann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Many definitions of resilience have been proffered for natural and engineered ecosystems, but a conceptual consensus on resilience in microbial communities is still lacking. We argue that the disconnect largely results from the wide variance in microbial community complexity, which range from compositionally simple synthetic consortia to complex natural communities, and divergence between the typical practical outcomes emphasized by ecologists and engineers. Viewing microbial communities as elasto-plastic systems that undergo both recoverable and unrecoverable transitions, we argue that this gap between the engineering and ecological definitions of resilience stems from their respective emphases on elastic and plastic deformation, respectively. We …

Surface Energy Balance, Evapotranspiration, And Surface Coefficients During Non-Growing Season In A Maize-Soybean Cropping System, Lameck O. Odhiambo, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Surface energy balance components, including actual evapotranspiration (ET), were measured in a reducedtill maize-soybean field in south central Nebraska during three consecutive non-growing seasons (2006/2007, 2007/2008, and 2008/2009). The relative fractions of the energy balance components were compared across the non-growing seasons, and surface coefficients (K_c) were determined as a ratio of measured ET to estimated alfalfa (ET_r) and grass (ET_o) reference ET (ET_ref). The non-growing season following a maize crop had 25% to 35% more field surface covered with crop residue as compared to the non-growing seasons following soybean crops. Net …

Control System Development And Response Analysis Of An Electronically Actuated Variable-Orifice Nozzle For Agricultural Pesticide Applications, Joe D. Luck, S. A. Shearer, M. P. Sama, Santosh Pitla

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The goal of this research project was to further the development of an electromechanically controlled variable-orifice nozzle by creating an electronic control system and then evaluating that system based on step and ramp inputs. The control system was developed in a programming environment that combined an electronic data acquisition system and actuator with pressure and flow sensors. A proportional, variable-gain (based on system pressure) control system was developed to adjust nozzle flow rates to meet target application rates. The constraints were to achieve settling time of less than 1.0 s, overshoot of less than 10% of maximum flow (or minimum …

Trihalomethane Removal And Re-Formation In Spray Aeration Processes Treating Disinfected Groundwater, Cassandra Smith

Electronic Theses and Dissertations

Historically, chlorination has been widely utilized as a primary and secondary disinfectant in municipal water supplies. Although chlorine disinfection is effective in inactivating pathogenic microbes, the use of chlorine creates the unintentional formation of regulated chemicals. On January 4, 2006, the United States Environmental Protection Agency (EPA) promulgated the Stage 2 Disinfectants/Disinfection by-product rule (DBPR) that focuses on public health protection by limiting exposure to four trihalomethanes (THM) and five haloacetic acids (HAA5), formed when chlorine is used for microbial pathogen control. This thesis examines post-aeration TTHM formation when employing spray-aeration processes to remove semi-volatile TTHMs from chlorinated potable water …

Flow, Spray Pattern, And Droplet Spectra Characteristics Of An Electronically Actuated Variable-Orifice Nozzle, Joe D. Luck, Santosh Pitla, M. P. Sama, S. A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The purpose of this study was to develop and evaluate the flow rate, spray pattern, and droplet spectra characteristics of an actively controlled variable-orifice nozzle at constant carrier pressures. A commercially available variable- orifice nozzle (VariTarget) was modified to allow for direct electromechanical control of the metering stem. The modified system was tested at five carrier pressures ranging from 138 to 414 kPa and five metering stem (and thus orifice) positions. The metering stem position range was chosen because it provided a linear response in flow rate at each carrier pressure. Flow rate testing indicated a turndown ratio of 2.4:1 …

Strengthening Black Cotton Soil With Fly Ash And Moorum: An Investigation Of The Role Of Subgrade And Subbase Layers, Gourav Saxena, Nikhlesh Chaurasia

Coal Combustion and Gasification Products Journal Archive

One of the most effective and economical methods to strengthen clayey soils is addition of stabilizing agents such as fly ash to the soil. In this study, highly plastic clay was stabilized using fly ash. Geotechnical properties such as Atterberg limits, unconfined compressive strength (UCS), and the California bearing ratio (CBR) value of virgin black cotton soil and soil treated with fly ash were evaluated. Soil was stabilized with various proportions of fly ash, i.e., at 0%, 10%, 15%, 20%, and 30%. The UCS of black cotton soil–fly ash mixes is found to be at its maximum at 20% fly …

Using Coal Fly Ash In Agriculture: Combination Of Fly Ash And Poultry Litter As Soil Amendments For Bioenergy Feedstock Production, E. Kudjo Dzantor, Ekundayo Adeleke, Vanaha Kankarla, Oluwatosin Ogunmayowa, Dafeng Hui

Coal Combustion and Gasification Products Journal Archive

Increasing fiscal, human, and environmental costs of coal fly ash (FA) management are leading to advocacy for greater beneficial uses of the by-products as soil amendments in agriculture. Greenhouse experiments were conducted in Armour silt loam (ASL) soil that was amended with FA (10%, wt/wt) with and without poultry litter (PL 5 75 mg N/kg). Biomass productivity of eastern gamagrass (GG), a warm-season perennial grass, was measured to serve as complementary biofuel feedstock to switchgrass (SG). FA was obtained from a site at the 2008 ash spill in Kingston, TN. GG and SG were grown individually in 15-cm-wide 3 41-cm-high …

Fabrication And Testing Of Low-Energy Calcium Sulfoaluminate-Belite Cements That Utilize Circulating Fluidized Bed Combustion By-Products, Robert Jewell, Robert Rathbone, Tristana Duvallet, Thomas Robl, Kamyar Mahboub

Coal Combustion and Gasification Products Journal Archive

The utilization of circulating fluidized bed combustion (CFBC) ash to make cement products that provide added value and offset CO2 production is the objective of this research. CFBC burns coal in the presence of a bed of slaked limestone, which effectively absorbs sulfur dioxide (SO2) to form anhydrite (CaSO4). CFBC produces two kinds of spent bed materials, coarse bottom ash and a much finer fly ash. Both of these products are very high in calcium. When properly conditioned, these materials are capable of acting as hydraulic cements, forming both calcium aluminosulfate minerals, most importantly ettringite, as well as calcium-alumina-silica gels, …

Prediction Of The Unburned Carbon Content Of Fly Ash In Coal-Fired Power Plants, Saeid R. Dindarloo, James C. Hower

Coal Combustion and Gasification Products Journal Archive

Prediction of carbon content in fly ash is a valuable index in both designing and optimizing coal-fired boilers. In this study, methods of multiple regression (MR), decision trees (DT), and support vector regression (SVR) were used for prediction of unburned carbon content of fly ash in a 300-MW power plant with a tangentially fired furnace using a low-NOx concentric firing system. Twenty-one boiler operational and coal petrological variables from 21 experiments in a previous benchmark study were used. A variables correlation matrix was used for feature (independent variables) selection in order to reduce the dimensions (number) of input variables from …

Recycling Of Lignite Coal Fly Ash By Its Conversion Into Zeolites, Silviya Boycheva, Denitza Zgureva, Annie Shoumkova

Coal Combustion and Gasification Products Journal Archive

The present study emphasized the conversion of fly ash from lignite coals into zeolites by a double-stage fusion-hydrothermal synthesis. The raw and the converted fly ashes were characterized with respect to their morphology, composition, and crystallinity. The effects of process parameters such as fusion temperature, concentration of the alkaline activator, and the duration of the hydrothermal incubation on the zeolitization process were studied. The experimental results indicate that the fusion temperature does not influence the process mechanism in the range 550–850uC, but it has an acceleration effect. The elevation of the sodium hydroxide/fly ash ratio from 1.6 to 2.0 enhances …

Modeling The Impact Of Future Climate On Drainage Infrastructures, Tyler J. Baumbach

Electronic Theses and Dissertations

Research has shown a potential 20% increase in future heavy and extreme precipitation events over the Midwestern States. Drainage infrastructures designed using current design conditions may not be able to convey projected runoffs resulting in flooding and damage to infrastructure. The objective of this paper is to determine the effects of future climate variability on culvert selections in a southwest South Dakota watershed. The scope of the study was defined through a comprehensive literature review. Future climate events were based on a 20% increase in current annual precipitation over the Upper White River Subbasin Watershed. A portion of the White …

Differences In Waste Generation, Waste Composition, And Source Separation Across Three Waste Districts In A New York Suburb, Omkar Aphale, Krista L. Thyberg, David J. Tonjes

Technology & Society Faculty Publications

Six tonnes of discards and recyclables from three waste districts in a New York suburb were sorted in 2012. The districts were chosen because one had a higher recycling percentage, one had median performance, and one was a low performing district. ASTM standards were followed for the waste composition sorting. The results showed, as expected, that the waste district with the highest recycling rate appeared to have the highest separation efficiencies, leading to greater amounts of recyclable materials being source separated. The waste districts also had different overall waste generation, both in terms of the amounts of wastes generated, and …

A Management Framework For Municipal Solid Waste Systems And Its Application To Food Waste Prevention, Krista L. Thyberg, David J. Tonjes

Technology & Society Faculty Publications

Waste management is a complex task involving numerous waste fractions, a range of technological treatment options, and many outputs that are circulated back to society. A systematic, interdisciplinary systems management framework was developed to facilitate the planning, implementation, and maintenance of sustainable waste systems. It aims not to replace existing decision-making approaches, but rather to enable their integration to allow for inclusion of overall sustainability concerns and address the complexity of solid waste management. The framework defines key considerations for system design, steps for performance monitoring, and approaches for facilitating continual system improvements. It was developed by critically examining the …

Quantification Of Food Waste Disposal In The United States: A Meta-Analysis, Krista L. Thyberg, David J. Tonjes, Jessica Gurevitch

Technology & Society Faculty Publications

Food waste has major consequences for social, nutritional, economic, and environmental issues, and yet the amount of food waste disposed in the U.S. has not been accurately quantified. We introduce the transparent and repeatable methods of meta-analysis and systematic reviewing to determine how much food is discarded in the U.S., and to determine if specific factors drive increased disposal. The aggregate proportion of food waste in U.S. municipal solid waste from 1995 to 2013 was found to be 0.147 (95% CI 0.137-0.157) of total waste, which is lower than that estimated by USEPA for the same period (0.176). The proportion …

Sustainable Disposal Of Coal Processing Waste Streams, Mohammad Rezaee

Theses and Dissertations--Mining Engineering

Modern coal preparation facilities incorporate a wide array of solid-solid and solid-liquid separation processes for rejecting mineral matter to meet market specifications. The coarse mineral matter is typically placed into engineered refuse piles whereas the fine refuse is either stored in impoundments or co-disposed with the coarse refuse. The discharge water from the refuse material represents an environmental concern due to the potential release of trace elements, and the subsequent elevation of total dissolved solids and conductivity. The research findings reported in this dissertation addresses sustainable coal processing waste disposal through using strategies aimed at minimizing the environmental impacts. To …

A Technical And Cultural Feasibility Assessment Of Household Microbial Fuel Cells For Use In Hanan'g District, Tanzania, Erinn Kunik

Dissertations, Master's Theses and Master's Reports

Microbial fuel cells (MFCs) are an emerging electricity generation technology that has the potential for use in developing countries. This study assessed the feasibility of using MFCs in Tanzania through technical analysis of a MFC prototype alongside with interviews with Tanzanians for a cultural feasibility assessment of MFCs. For the technical analysis, this study evaluated the inputs needed for a household MFC to operate and produce electricity with cattle manure as a fuel and evaluated the electrochemical and chemical performance as well as the fecal coliform reduction of the manure during MFC operation. For this study, a MFC was built …

Pathogen Indicator Monitoring In The Ross Barnett Reservoir, Hannie Quay Parker Capps

Electronic Theses and Dissertations

Man-made reservoirs are often used for both water supply and recreation. The Ross Barnett Reservoir in central Mississippi, a 33,000-acre man-made lake, provides drinking water to the city of Jackson, MS, and forty-eight surrounding communities. Further, an estimated 2.5 million people visit the Reservoir each year for recreational purposes, including boating, fishing, water-skiing, and swimming. Protecting the water quality in the Reservoir is important for these visitors and inhabitants along the shoreline, and for these reasons, the U.S. Environmental Protection Agency has selected it as a Priority Watershed in Mississippi. Presently, there is a concern regarding recent data collected that …