Physical Sciences and Mathematics Commons^™

Environmental Variability Effects On Marine Fisheries: Four Case Histories, Eileen E. Hofmann, T. M. Powell

CCPO Publications

The changing nature of marine fisheries requires management approaches that recognize and include ecosystem and environmental effects. Therefore, we review some examples of exploited fishery stocks in which environmental control is a major contributor to structuring the abundance and distribution of the stock. Four examples, taken from studies of northern cod (Gadus morhua), cod and haddock (Melanogrammus aeglefinus) larvae, the eastern oyster (Crassostrea virginica), and Antarctic krill (Euphausia superba), are given that clearly illustrate environmental control on the fishery. From these examples, we argue that future management strategies for exploited fisheries must include effects of environmental variability. In particular, management …

Seasonal Patterns Of Nitrogen Fixation In Termites, A. D. Curtis, D. A. Waller

Biological Sciences Faculty Publications

Summary

1. Termite nitrogenase activity was highest in autumn and spring (≈ 3 µg N₂ fixed termite fresh mass (g)^–1 day^–1) and lowest in winter and summer (≈ 0·8 µg N₂ fixed termite fresh mass (g)^–1 day^–1).

2. The nitrogenase activity of worker termites was significantly higher than all other castes (1·58 ± 0·27 µg N₂ fixed termite fresh mass (g)^–1 day^–1).

3. Worker termites constituted the largest proportion of all the castes throughout the study period (≈ 90%).

4. The localized input of fixed nitrogen by termites …

Mesozooplankton Distribution And Abundance In The Pagan River: A Nutrient Enriched Subestuary Of The James River, Virginia, Lillian N. Davis, Harold G. Marshall

Biological Sciences Faculty Publications

The mesozooplankton in the Pagan River was dominated by calanoid copepods, with abundance peaks occurring during late winter-early spring and from late summer into early fall. This included spring, summer, and fall abundance maxima. The total mean abundance of the mesozooplankton was 3,008/m³.

Lead Isotopes And Selected Metals In Ice From Law Dome, Antarctica, K. J. R. Rosman, W. Chisholm, C. F. Boutron, S. Hong, R. Edwards, Vin Morgan, Peter N. Sedwick

OES Faculty Publications

The isotopic composition. of Pb and the concentrations of Pb, Ba and Bi were measured in selected ice-core samples from Law Dome, East Antarctica, to a depth of 1196 m. The range of concentrations found in decontaminated ice was 0.03-1.5 pg g^-1 for Pb, 0.9-6.1 pg g^-1 for Ba and 0.4-17 fg g^-1 for Bi, excluding the deepest sample which contained ∼1 ppm of rock dust. The abundances of all four stable lead isotopes were measured and gave ²⁰⁶Pb/²⁰⁷Pb ratios ≤ ∼1.23. A value of ²⁰⁸Pb/²⁰⁷Pb = 2.78 was measured in …

Phytoplankton Composition Within The Tidal Freshwater Region Of The James River, Virginia, Harold G. Marshall, Lubomira Burchardt

Biological Sciences Faculty Publications

Based on a 10.5 year data set, 271 taxa were identified at a single tidal freshwater station in the James River. The mean monthly concentrations of major algal categories, total biomass and productivity are given. Diatom maxima were associated with peak periods of river discharge, with chlorophytes, cyanobacteria, and autotrophic picoplankton abundance and productivity greater during reduced river flow and more stable water conditions.

Evaluation Of Toxicity, Bioavailability And Speciation Of Lead, Zinc And Cadmium In Mine/Mill Wastewaters, Mujde Erten-Unal, Bobby G. Wixson, Nord Gale, Jerry L. Pitt

Civil & Environmental Engineering Faculty Publications

The toxicity of common compounds of lead, cadmium and zinc was evaluated in waters similar to that found in the world's largest lead producing area in Missouri. Static, acute toxicity tests were performed using fathead minnows (Pimephales promelas) and water fleas (Daphnia magna) respectively. Test organisms were subjected to varying amounts of sulfide, carbonate, chloride and sulfate salts of lead, zinc and cadmium mixed in hard, alkaline waters typical to this region. Median lethal concentrations were calculated using nominal versus measured metal concentrations. Measured metal concentrations included four different metal fractionation (extraction/filtration) techniques at different pH …

The Detoxification Of Petroleum Contaminated Coastal Plain Sandy Soil Using An Amended Vermicomposting Approach, John Charles Kraemer

Health Services Research Dissertations

This study explores the feasibility of utilizing an amended vermicomposting treatment approach for detoxifying petroleum hydrocarbon contaminated sandy soil. The bench-scale testing of gasoline and diesel contaminated soil using three test soil vessels and one control soil vessel was performed in a laboratory setting for a six week time period. The control soil received no treatment other than distilled water to maintain soil moisture. Treatment 1 consisted of direct application of liquid municipal biologic sludge on a weekly basis. Treatment 2 consisted of the addition of 30 Eisenia foetida earthworms and distilled water. Treatment 3 consisted of direct application of …

Stomach Content Analysis Of Stranded Bottlenose Dolphins, Tursiops Truncatus, In Virginia, 1987-1996, John Mcgurk

Biological Sciences Theses & Dissertations

I examined the stomach contents from 94 bottlenose dolphins, Tursiops truncatus, that were stranded along the Virginia coastline from 1987 through 1996. Samples from 1987 through 1991 were obtained from the National Museum of Natural History (NMNH) and samples from 1992 through 1996 were collected by the Virginia Marine Science Museum (VMSM) Stranding Team. Prey species were identified using fish otoliths and cephalopod beaks.

The dolphins examined fed on 23 species of fish and one genus of cephalopods. The primary prey species was Atlantic croaker (Micropogonias undulatus); secondary species were weakfish (Cynoscion regalia), spot (Leiostomus xanthurus), silver perch (Bairdiella chrysoura), …

Evaluation Of Solid Phase Extraction Followed By Supercritical Fluid Elution Of Semi-Volatile Organic Compounds In Toxicity Characteristic Leachates, William Edward Corl Iii

Chemistry & Biochemistry Theses & Dissertations

The Environmental Protection Agency (EPA) developed the toxicity characteristic leaching procedure (TCLP) to determine the potential for unknown wastes to leach haz.ardous constituents into the environment. Methods currently being used for the extraction of semi-volatile organic compounds from aqueous TCLP leachates involve acid/base partitioning into methylene chloride. The concentrated extract is then analyzed by gas chromatography/mass spectrometry (GC/MS). The methods are either timeconsuming or labor intensive and they generate significant volumes of haz.ardous wastes themselves. Considering the haz.ards and disposal costs of methylene chloride wastes, great interest has been elicited by the EPA in other extraction techniques which would reduce …

Development Of A Fluorescent Indicator For Inorganic Monochloramine, Xiaojun Dong Zou

Chemistry & Biochemistry Theses & Dissertations

Current methods used to determine combined residual chlorine cannot distinguish between inorganic monochloramine (NH₂Cl) and organic chloramines. Studies described in this thesis were carried out to develop a novel method of determining the concentration of inorganic monochloramine in aqueous solutions. Based on the observation that the reaction of 3-formyl-1-methylpyridinium chloride (3FMPCl) with NH₂Cl produces a solution which is fluorescent, experiments were conducted to characterize the reaction and optimize the experimental conditions. Using the finalized protocol, NH₂Cl measurements were made in the presence and absence of organic amines and compared with a commonly used N, …

Councilmanic Perceptions Of Water Policy For The Lake Gaston Water Supply Project In Virginia And North Carolina, Beverly Mcafee Hedberg

Theses and Dissertations in Urban Services - Urban Management

The purpose of this case study is to analyze the perceptions of city council members in formulating and implementing policy on the Lake Gaston Water Supply Project in Virginia and North Carolina. A comparison of the perceptions of twenty-three members of three city councils: Henderson, North Carolina; Roanoke Rapids, North Carolina; and Virginia Beach, Virginia serves as the basis for analysis. Their perceptions of the issues and how they view other actors in the intergovernmental arena, within the context of the Lake Gaston Water Supply Project.

Specifically, the research examines how city council members view (1) water-policy issues, (2) the …

Microzooplankton Dynamics In Lower Chesapeake Bay And Its Major Tributaries, Gyung Soo Park

Biological Sciences Theses & Dissertations

This study identified the temporal and spatial variability of microzooplankton density and biomass at 14 stations located within Chesapeake Bay and four rivers. Microzooplankton are defined as those heterotrophic organisms which range from 20 to 200 $\mu$m in size, and in this study were mainly composed of copepod nauplii, rotifers, and ciliates. Ciliates were the most abundant microzooplankton, comprising more than 90% of the total density, with copepod nauplii representing approximately 50% of the total microzooplankton biomass (carbon content). Rotifers contributed less than 5% for both density and biomass of the total microzooplankton.

Maximum ciliate abundance occurred from spring to …

Seasonal Abundance Of Autotrophic Picoplankton In The Pagan River, A Nutrient Enriched Subestuary Of The James River, Virginia, Lillian N. Davis, Karen A. Phillips, Harold G. Marshall

Biological Sciences Faculty Publications

Autotrophic picoplankton had average monthly concentrations of 73.5 X 10⁶ cells/L in the Pagan River, with summer-early fall maxima of 10⁸ cells/L. The abundance peaks increased with rising water temperatures, declining to their least abundance in mid-winter (10⁵ cells/L).

A Test Of An Alternate Calibration Matrix For Niosh Method 7300, Robert T. Vitek

Community & Environmental Health Theses & Dissertations

The purpose of this study was to determine if the analytical results (when determining cadmium, lead, and zinc concentrations) for air sample filters prepared and analyzed according to N:OSH Method 7300, differ significantly, from those obtained when the instrument is calibrated using EPA Method 200.7 acid matrix standards. The null hypothesis was that no statistically significant difference, measured at the 95% confidence level, would exist between results obtained from the two calibration standards. Careful standard and sample preparation, the use of an internal standard, and the application of working standard and working internal standard correction factors served to isolate the …

Comparison Of Sapling Community Structure In Created And Natural Forested Wetlands In Southeastern Virginia, Sharon Ann Haines

Biological Sciences Theses & Dissertations

Forested wetlands are being destroyed at a rapid rate. Wetlands are valuable for flood control, aquifer recharge, and for their role in denitrification and storage of global carbon. When wetlands are lost, their functions and value to humans are lost as well. One response to the continued development of wetlands has been the artificial creation of new wetlands. This study compared the species density, relative density, coverage and frequency of occurrence of the woody vegetation in two southeastern Virginia created wetlands with that in two reference wetlands, also in southeastern Virginia, to determine the structural similarity of their respective communities. …

Development And Lipid Composition Of The Harpacticoid Copepod Nitocra Spinipes Reared On Different Diets, Gregory M. Weiss, George B. Mcmanus, H. Rodger Harvey

OES Faculty Publications

We reared the harpacticoid copepod Nitocra spinipes on diets of bacteria, a diatom, or a macroalga, evaluating survivorship and growth in short-term (≤ 1 generation) experiments. Lipid content of the copepods and their diets was measured and used as an index of nutrition. Although growth, survivorship and lipid content of N. spinipes were significantly greater when fed the diatom, which had the highest lipid content of the 3 diets, the copepod was able to develop from egg to adult when fed a lipid- poor bacterial diet. Furthermore, this species was able to go through developmental molts without the addition of …

Trophic Transfer Of Fatty Acids, Sterols, And A Triterpenoid Alcohol Between Bacteria, A Ciliate, And The Copepod Acartia Tonsa, Melissa C. Ederington, George B. Mcmanus, H. Rodger Harvey

OES Faculty Publications

The incorporation of lipids into the copepod Acartia tonsa and its eggs was measured when it was fed either a bacterivorous ciliate (Pleuronema sp.) or a diatom (Thalassiosira weissflogii). Egg production was 10-fold higher on the diatom diet, whereas hatch success of eggs was the same for algal and ciliate diets. Adult copepods fed diatoms contained more total fatty acid and sterols than copepods fed the ciliate diet, and individual lipids reflected the dietary source. Eggs from diatom-fed copepods had fewer fatty acids but more sterols than eggs from copepods on a ciliate diet. Ciliate-fed copepods and …

Zooplankton Community Dynamics In The Elizabeth River, Virginia, William C. Sharp

Biological Sciences Theses & Dissertations

Zooplankton community structure and dynamics were studied over a 12 month period in the Elizabeth River, an industrially polluted tidal river in the Chesapeake Bay estuarine system. An additional site in this estuary (Hampton Roads) was sampled for comparative purposes. Multivariate analysis indicated five distinct temporal groups occurred during the year, but there were no significant differences in spatial composition within the river. Maximum zooplankton concentrations were observed in July and lowest abundances were observed in February, March, and November. Copepods, especially Acartia species, were the most abundant components of the zooplankton community, comprising 67 to 93% of the total …

Time To Metamorphosis Of Blue Crab Callinectes Sapidus Megalopae: Effects Of Benthic Macroalgae, Robert D. Brumbaugh, John R. Mcconaugha

OES Faculty Publications

There is growing evidence that postlarvae (megalopae) of the blue crab Callinectes sapidus can slow the progression through the molt cycle while in offshore nursery grounds, and delay metamorphosis until reinvasion of coastal habitat occurs. However, the cues that trigger metamorphosis of megalopae are not well known. This study tested the hypothesis that the time to metamorphosis (TTM) from the postlarval megalops stage to the first crab stage is shortened in the presence of 2 potential macroalgal settlement substrates, Ulva lactuca (Chlorophyta), and Gracilaria spp. (Rhodophyta). Megalopae and test water were collected from 3 locations (offshore, at a coastal inlet, …

The Pollution Effects Of Pleasure/Work Boat Sewage Discharges On Fecal Contamination Levels Of Waters In And Around Marinas And Other Places Where Boats Are Moored On The Eastern Shore Of Virginia, George H. Bangs

Community & Environmental Health Theses & Dissertations

Contamination of seafood harvested from and recreational areas of the Chesapeake Bay, its tributaries and the Atlantic coast inshore waters may cause health problems for people consuming the seafood or coming in contact with the waters by swimming, water skiing, or other water sports. These health problems include, but not limited to, dysentery, typhoid and hepatitis (Natchez, 1990). This is a preliminary study into the level of fecal pollution generated by sewage discharges from boats in marinas and other places where boats are moored on the Eastern Shore of Virginia. Sites selected for the study included; (I) marinas and other …

The Speciation Of Dissolved Uranium In Marine Waters, Darrin Kent Mann

OES Theses and Dissertations

Uranium has been used extensively for quantifying geochemical processes in the marine environment. Due to its low concentration, about 3.3 μg/1, some form of preconcentration is required for analysis. The two most widely used pre-concentration schemes are co-precipitation with iron hydroxide, and chelating ion exchange chromatography. These methods were developed by following the behavior of inorganic uranium isotopes and seemed to give identical results. However, when used for trace metal analysis, these methods have been shown to extract different fractions from the same sample. Chelex-100 resin removes only inorganic or weakly bound trace metals from a solution, while the precipitates …

Materials Recovery Facilities In The United States Virgin Islands: A Regional Facilities Location Model Study, Lloyd O. Prince Jr.

Engineering Management & Systems Engineering Theses & Dissertations

Continental municipalities have derived many benefits from the economies of scale associated with a regional approach to facilities location and management planning. Centralized solid waste processing facilities is an example. Island communities, however, surrounded by miles of ocean, are constrained to a fragmented approach to the facilities location solution. This research was conducted to determine if the regional paradigm suggested in the literature is applicable to a set of island communities connected by an ocean transportation infrastructure. A linear programming (LP) model, constraints and data requirements were developed and applied to a network of islands. A series of hypothetical material …

Modeling Daily Production Of Aquatic Macrophytes From Irradiance Measurements: A Comparative Analysis, Richard C. Zimmerman, Alejandro C. Pasini, Randall S. Alberte

OES Faculty Publications

The importance of submerged aquatic macrophytes to coastal ecosystems has generated a need for knowledge of minimum light levels that will support the maintenance and restoration of healthy populations. Our goals were (1) to evaluate the sensitivity to natural, non-sinusoidal fluctuations in irradiance I of analytical integration techniques for calculating daily carbon gain, (2) to evaluate the H_sat (the daily period of I-saturated photosynthesis) model of daily production relative to models based on instantaneous photosynthesis vs irradiance (P vs I) and (3) to provide some guidance for the temporal density of irradiance data required for accurate estimation of …

A Study Of The Chlorination Products Of Methionine In Model Solutions And In Wastewater, D. Scott Winters

Chemistry & Biochemistry Theses & Dissertations

Model solutions of the amino acid methionine were chlorinated to seven chlorine-to-nitrogen (Cl₂/N) molar ratios. After 30 minutes, the solutions were analyzed by high performance liquid chromatography (HPLC). At a Cl₂/N molar ratio of 1, methionine sulfoxide was found to be the main product. At Cl₂/N molar ratios ≥ 2, a mixture of methionine sulfoxide, methionine sulfone, an unidentified sulfoxide (product 1), and N-chloro-3-(methylsulfonyl) propionaldimine are formed. These products were identified by comparison to synthesized standards which were characterized by ¹H-NMR and ¹³C-NMR nuclear magnetic resonance spectroscopy, ion trap mass spectroscopy, …

Chlorination Products Of Glycylphenylalanine In Model Solutions And A Wastewater, Daniel J. Keefe

Chemistry & Biochemistry Theses & Dissertations

Model solutions of the dipeptide glycylphenylalanine were chlorinated to five different chlorine-to-amino nitrogen (Cl/N) mole ratios and analyzed after 30 min by high performance liquid chromatography. At mole ratios of ≤ 1, N-chloroglycylphenylalanine (I) appeared to be the only major product. At a mole ratio ≤ 2, N, N-dichloroglycylphenylalanine (II) was the only product. Compound II decomposes in model solutions (t_1/2= 6.4 hr) at pH 7.0 to form a compound tentatively identified as an H-chloroaldimine (III). Compound III in turn decomposes (t_1/2= 36 hr) in model solutions at pH 7. o to (IV) . From ^{13 …}

Changes In The Sea-Ice Brine Community During The Spring-Summer Transition, Mcmurdo Sound, Antarctica .2. Phagotrophic Protists, Diane K. Stoecker, Kurt R. Buck, Mary Putt

OES Faculty Publications

The land-fast sea-ice brine contains a diverse phagotrophic protist assemblage consisting of < 5 mum heterotrophic flagellates, Cryothecomonas spp., heterotrophic dinoflagellates, and heterotrophic and mixotrophic ciliates. Fine-scale horizontal spatial variability is a feature of this assemblage; samples taken within 1 m of each other can be dominated by different heterotrophic protists. Many of the larger heterotrophic protists found in the brine are also found in the water column. The photosynthetic ciliate Mesodinium rubrum is also common. In mid to late austral spring, the heterotrophic assemblage accounts for ca 10% of the total protist biomass in the brine and is dominated by Cryothecomonas spp. This flagellate …

Benthic Microalgal Production At Stellwagen Bank, Massachusetts Bay, Usa, Lawrence B. Cahoon, Guy R. Beretich Jr., Carrie J. Thomas, Amy M. Mcdonald

OES Faculty Publications

Benthic microalgal chlorophyll a and production were measured at 3 sites at Stellwagen Bank, a cold temperate continental shelf habitat in Massachusetts Bay, USA, during August 1991. Benthic microalgal chlorophyll a averaged 39.8 Mg M^-2, vs average integrated phytoplankton chlorophyll a of 25.9 mg m^-2. Gross benthic microalgal production, measured by oxygen exchange in clear and opaque benthic chambers, averaged 20.9 mg C m^-2 h^-1. This production was supported by average daily light fluxes to the bottom that never exceeded 1 % of surface incident radiation and were as low as 4.7 μE …

Molecular And Physiological Responses Of Diatoms To Variable Levels Of Irradiance And Nitrogen Availability: Growth Of Skeletonema Costatum In Simulated Upwelling Conditions, G. Jason Smith, Richard C. Zimmerman, Randall S. Alberte

OES Faculty Publications

Molecular mechanisms that drive metabolic acclimation to environmental shifts have been poorly characterized in phytoplankton. In this laboratory study. the response of light- and N-limited Skeletonema costatum cells to an increase in light and NO₃ availability was examined. C assimilation was depressed relative to N assimilation early in enrichment, and the photosynthetic quotient (O₂: CO₂) increased, consistent with the shunting of reducing equivalents from CO₂ fixation to NO₃- reduction. The concomitant increase in dark respiration was consistent with the increased energetic demand associated with macromolecular synthesis. The accelerations of N-specific rates of …

Development And Standardization Of A Short-Term Assay For Evaluating Polluted Estuarine And Coastal Environments: The Medaka Embryo-Larval Assay, Michael Frederick Helmstetter

OES Theses and Dissertations

The eggs of the Japanese medaka (Oryzias latipes) were employed in a number of studies to develop a routine, standardized assay which can assess the acute and sublethal impacts of individual toxicants and complex mixtures. The eggs of this Cyprinodont minnow were topically treated with each toxicant or mixture dissolved in membrane permeable dimethylsulfoxide (DMSO) solvent. Nine chemicals were initially evaluated with two, pentachlorophenol (PCP) and tributyltin chloride (TBTCl), subsequently selected for extensive evaluations of salinity tolerance and the accuracy of the topical exposure method for representing a typical immersion exposure. The amount of material actually penetrating the eggs was …

Community Structure Of The Macrobenthos In Back Bay, Virginia, Michael F. Lane

Biological Sciences Theses & Dissertations

A study of the subtidal macrobenthos in Back Bay, Virginia was conducted to examine community structure in relation to sedimentary and water quality characteristics. Samples were collected in August and November of 1987 and February and May of 1988 at ten stations.

Three site groups and three temporal groups were identified by cluster analyses of species abundance. Species composition between site groups was relatively homogeneous and eight species accounted for most of the variation between site groups. A comparison of plots of the biological and physical data in discriminant space suggested that variations in the biological data between site groups …