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1994

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Full-Text Articles in Medicine and Health Sciences

Comparison Of Peritoneal Fluid Analysis Before And After Exploratory Celiotomy And Omentopexy In Cattle, David Anderson, D Cornwell, G St-Jean, A Desrochers, Lane Anderson Feb 2013

Comparison Of Peritoneal Fluid Analysis Before And After Exploratory Celiotomy And Omentopexy In Cattle, David Anderson, D Cornwell, G St-Jean, A Desrochers, Lane Anderson

Lane S Anderson, DVM

The effect of right paralumbar fossa exploratory celiotomy and omentopexy on peritoneal fluid constituents was studied in 22 adult dairy cows. Six cows were eliminated on the basis of physical examination findings (n = 2), surgical findings (n = 2), or inability to obtain a sufficient volume of peritoneal fluid (n = 2). Sixteen cattle had normal results of CBC and serum biochemical analysis, and a minimum of 1 ml of peritoneal fluid was obtained by abdominocentesis. Abdominocentesis was repeated on days 1, 2, and 6 after surgery. Statistical analysis for repeated measures was performed, using a significance level of P < 0.05. Stage of gestation was evaluated for interaction with time. Mean total nucleated cell count was 3,200 cells/microliters before surgery, was significantly increased 2 days after surgery (16,336 cells/microliters), and continued to increase through day 6 (20,542 cells/microliters). Mean polymorphonuclear cell count was 1,312 cells/microliters before surgery and was significantly higher at 2 (11,043 cells/microliters) and 6 (10,619 cells/microliters) days after surgery. Mean lymphocyte count was 254 cells/microliters before surgery and was significantly increased 2 days (1,911 cells/microliters) after surgery. By day 6, lymphocyte numbers were similar to preoperative values. Mean mononuclear cell count was 770 cells/microliters before surgery and was significantly increased on days 1 (3,084 cells/microliters), 2 (3,285 cells/microliters), and 6 (2,349 cells/microliters) after surgery. Mean eosinophil numbers were 1,388 cells/microliters before surgery and were significantly increased on day 6 (6,347 cells/microliters) only. Interaction between time and stage of gestation was found only for specific gravity and total protein concentration.(ABSTRACT TRUNCATED AT 250 WORDS)


High-Performance Liquid Chromatographic Determination Of Intrathecally Administered [D-Ala2-D-Leu5]-Enkephalin Concentrations In Canine Cerebrospinal Fluid, Sherry Cox, Ralph Harvey, Donita Frazier Jun 2011

High-Performance Liquid Chromatographic Determination Of Intrathecally Administered [D-Ala2-D-Leu5]-Enkephalin Concentrations In Canine Cerebrospinal Fluid, Sherry Cox, Ralph Harvey, Donita Frazier

Sherry Cox

A rapid and useful method for high-performance liquid chromatographic analysis of exogenous [d-Ala2-d-Leu5]-enkephalin (DADLE) in cerebrospinal fluid (CSF) is described. CSF (0.5 ml) samples were filtered using a 0.22-μm Co-Star filter. Chromatography was performed on a μBondapak C18 column using a mobile phase of A, 0.05 M sodium phosphate (monobasic, pH 6.0) and B, 60% acetonitrile in 0.05 M sodium phosphate (pH 6.0) with a flow-rate of 1 ml/min. Absorbance at 210 nm was measured. The procedure produced a linear curve for the concentration range 1–10 μg/ml. The development of ...


Retiree Dies After Tractor Overturns Into Creek, Kentucky Injury Prevention And Research Center Dec 1994

Retiree Dies After Tractor Overturns Into Creek, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 58-year-old retired factory worker and part-time farmer was killed when the tractor he was operating overturned into a creek and pinned him. At the time of the incident, the victim was mowing a pasture along a creek bed. The tractor was not equipped with a Roll Over Protective Structure (ROPS) or a seat belt. Attached to the tractor was a five foot bush hog. About 3:00 pm the day of the incident, the victim began mowing a 30 acre bottom land pasture by entering a gate at the east of the pasture. The victim drove in a westward ...


Klinik Jual Obat Aborsi Di Subang 081215265852 Jual Obat Aborsi Cytotec Di Subang, Tiara Shoop01 Dec 1994

Klinik Jual Obat Aborsi Di Subang 081215265852 Jual Obat Aborsi Cytotec Di Subang, Tiara Shoop01

Jual Obat Aborsi - Obat Cytotec Penggugur Kandungan Ampuh Tuntas

Klinik Aborsi Penggugur Kandungan Ampuh Subang, Penjual Obat Penggugur Kandungan Di Area Subang, Obat Aborsi Di Subang, Pesan Obat Aborsi Di Subang, Obat Penggugur Kandungan di Subang, Obat Aborsi Di Apotik Subang, Obat Telat Datang Bulan Di Subang, jual obat aborsi daerah Subang


Obat Aborsi Kulon Progo 0812-1526-5852 Alamat Klinik Jual Obat Aborsi Cytotec Di Kulon Progo, Tiara Shoop01 Dec 1994

Obat Aborsi Kulon Progo 0812-1526-5852 Alamat Klinik Jual Obat Aborsi Cytotec Di Kulon Progo, Tiara Shoop01

Jual Obat Aborsi - Obat Cytotec Penggugur Kandungan Ampuh Tuntas

Penjual Obat Penggugur Kandungan di Kulon Progo, Obat Aborsi Di Apotik Kulon Progo, Obat Telat Datang Bulan Di Kulon Progo, Jual Obat Aborsi Di Kulon Progo, Obat Aborsi Kulon Progo, Obat Aborsi Di Kulon Progo, Obat Telat Bulan Kulon Progo, Jual Obat Aborsi Kulon Progo


Increasing Nurse's Knowledge Of Neonatal Pain Assessment Through Inservice Education Program, Carol Beloff Dec 1994

Increasing Nurse's Knowledge Of Neonatal Pain Assessment Through Inservice Education Program, Carol Beloff

FIU Electronic Theses and Dissertations

Recognizing neonatal pain is a challenge for nurses working with newborns due to the complexity of the pain phenomenon. Pain is subjective, and infants lack the ability to communicate, and their pain is difficult to recognize. The purpose of this study is to determine the effectiveness of education on the NICU nurses' ability to assess neonatal pain. With a better understanding of pain theory and the effects of pain on the newborn the nurse will be better able to assess newborns with pain. Designed as a quasi-experimental one-group pretest and posttest study, the data was collected on a convenience sample ...


Part-Time Farmer Is Killed In Rops-Equipped Tractor Rollover, Kentucky Injury Prevention And Research Center Dec 1994

Part-Time Farmer Is Killed In Rops-Equipped Tractor Rollover, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 60-year-old male minister and part-time farmer began mowing a 15-acre field near his home at about 11:00 am. Operating a farm tractor with a side sickle bar mower attached to the right side, he proceeded around the perimeter of the field. One edge of the field dropped off steeply to a ravine. While making the second pass around the field the ground under the left wheels gave way causing the tractor to roll over. The tractor came to rest on the victim causing massive internal injuries. A Roll Over Protective Structure (ROPS) had been retro-fitted to the 1962 ...


Farmer Pinned When Tractor Overturns Into Dry Creek Bed, Kentucky Injury Prevention And Research Center Dec 1994

Farmer Pinned When Tractor Overturns Into Dry Creek Bed, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 65-year-old farmer died after his tractor overturned, pinning him underneath. The victim, working alone, began mowing with a bush hog along the perimeter of a soy bean field. At approximately 12:30 pm, the victim was about 1/4 mile from the main road mowing blackberry briars along a creek bed. This area was between the bean field and a creek embankment. Having made two cuts into the brush about 30 feet apart for the purpose of marking the creek edge, he proceeded to make a third cut. He backed the tractor, bush hog attached, toward the embankment. The ...


Farm Worker Is Killed In Tractor Rollover, Kentucky Injury Prevention And Research Center Dec 1994

Farm Worker Is Killed In Tractor Rollover, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 42-year-old farm worker was killed when the tractor he was operating rolled over on him. The tractor was not equipped with Roll Over Protective Structures (ROPS) or a seat belt. The victim had been bush hogging alone in a field the morning of the incident. Having completed a lower pasture, he proceeded up a single lane loose gravel road carved in the side of the hill. The left side going up the hill was covered with trees and brush. The right side fell quickly off to form a steep embankment. As the victim maneuvered the tractor up the hill ...


Daughter Cells Of Saccharomyces Cerevisiae From Old Mothers Display A Reduced Life Span, Nicanor Austriaco, Brian K. Kennedy, Leonard Guarente Dec 1994

Daughter Cells Of Saccharomyces Cerevisiae From Old Mothers Display A Reduced Life Span, Nicanor Austriaco, Brian K. Kennedy, Leonard Guarente

Biology Department Faculty Publications

The yeast Saccharomyces cerevisiae typically divides asymmetrically to give a large mother cell and a smaller daughter cell. As mother cells become old, they enlarge and produce daughter cells that are larger than daughters derived from young mother cells. We found that occasional daughter cells were indistinguishable in size from their mothers, giving rise to a symmetric division. The frequency of symmetric divisions became greater as mother cells aged and reached a maximum occurrence of 30% in mothers undergoing their last cell division. Symmetric divisions occurred similarly in rad9 and ste12 mutants. Strikingly, daughters from old mothers, whether they arose ...


Cytokines And Ovulation In The Mouse Ovary, Jong G. Kim Dec 1994

Cytokines And Ovulation In The Mouse Ovary, Jong G. Kim

Electronic Theses and Dissertations

Ovulation has been hypothesized as an inflammatory process. Interleukin(IL)-1$\alpha$, IL-1$\beta$ and tumor necrosis factor(TNF)-$\alpha$ are potent cytokines produced from macrophages and various other cell types, and are pivotal components of inflammation. Although previous studies have investigated cytokine activities in the reproductive system, there is little information on their precise localization and activities during the periovulatory period. To investigate the role of cytokines in ovulation, experiments were designed to determine the immunohistochemical localization and time specific production of cytokines IL-1 and TNF-$\alpha$ using a mouse model at 36h, 12h, 6h, 2h before ovulation, and ...


Microcirculation: Electrophysiological Basis For The Response Of Endothelial Cells To Inflammatory Mediators-Bradykinin, Kai Miao Dec 1994

Microcirculation: Electrophysiological Basis For The Response Of Endothelial Cells To Inflammatory Mediators-Bradykinin, Kai Miao

Electronic Theses and Dissertations

Using conventional microelectrodes, I studied the electrical basis for determining the resting V$\sb{\rm m}$ in intact EC's from hamsters. The resting V$\sb{\rm m}$ were found to be $-$40 mV for aortic EC's and $-$43 mV for vena caval EC's. The contributions of ions to the resting V$\sb{\rm m}$ of aortic EC's were compared in terms of the transference number (t$\sb{\rm ion}$). To develop a technique for in situ monitoring changes in V$\sb{\rm m}$ of postcapillary venular EC's in the hamster mesentery, a voltage-sensitive fluorescent probe ...


Women’S-Perceived Quality In Postpartum Care, Maryland A. Hunter Dec 1994

Women’S-Perceived Quality In Postpartum Care, Maryland A. Hunter

Theses and Dissertations (ETD)

Increased competition among healthcare organizations for the obstetric consumer market has led to increased interest in women’s perceptions of their obstetric experience. This study explored women’s perceptions of quality and women’s perceptions of benefits for women who received family-centered postpartum care (FCPPC) and women who received traditional postpartum care (TPPC). The study also assessed the relationship between women’s-perceived quality (WPQ) and women’s-perceived benefit (WPB) for those receiving FCPPC and those receiving TPPC. Quality and beneficence are the two concepts of Larrabee’s (1992) model of quality operationalized in this study. The sample consisted of 60 ...


The Effects Of Elevated Glucose Upon Na+/K+-Atpase In Bovine Retinal Pigment Epithelial Cells, Julie Y. Crider Dec 1994

The Effects Of Elevated Glucose Upon Na+/K+-Atpase In Bovine Retinal Pigment Epithelial Cells, Julie Y. Crider

Theses and Dissertations

Crider, Julie Y., The Effects of Elevated Glucose Upon Na+/K+-ATPase in Bovine Retinal Pigment Epithelial Cells. Doctor of Philosophy (Biomedical Sciences, Pharmacology), December, 1994, 154 pp., 14 tables, 31 illustrations, bibliography, 288 titles. Bovine retinal pigment epithelial (RPE) cells were cultured under 1, 4.5 and 10 g/l glucose conditions in order to characterize the effects of hyperglycemia upon Na+/K+-ATPase. Functional activity of Na+/K+-ATPase was measured as ouabain-sensitive Rb+ uptake. 3H ouabain was used to assess binding characteristics of Na+/K+-ATPase. The major contributors to rubidium (mRb+) uptake activity were the ouabain-sensitive ...


Texas D.O. Volume 51, Number 11, Texas Osteopathic Medical Association Dec 1994

Texas D.O. Volume 51, Number 11, Texas Osteopathic Medical Association

Texas Osteopathic Physicians Journal

No abstract provided.


Dependence Of Thiol-Stimulated K-Cl Cotransport On Redox State Of Low K Sheep Red Blood Cells, Peter K. Lauf, Norma C. Adragna, Nihal S. Agar Dec 1994

Dependence Of Thiol-Stimulated K-Cl Cotransport On Redox State Of Low K Sheep Red Blood Cells, Peter K. Lauf, Norma C. Adragna, Nihal S. Agar

Pharmacology and Toxicology Faculty Publications

No abstract provided.


Update - December 1994, Loma Linda University Center For Christian Bioethics Dec 1994

Update - December 1994, Loma Linda University Center For Christian Bioethics

Update

In this issue:

-- Minding Our Language: Metaphors and Biomedical Ethics


Defining Success: The Politics Of Evaluation In Alcohol And Drug Abuse Treatment Programs, James L. Wolk, David J. Hartmann, William P. Sullivan Dec 1994

Defining Success: The Politics Of Evaluation In Alcohol And Drug Abuse Treatment Programs, James L. Wolk, David J. Hartmann, William P. Sullivan

The Journal of Sociology & Social Welfare

Alcohol and drug abuse treatment programs must respond to several important stakeholders or beneficiaries of services who have an investment in how success is defined. Utilizing data from recent statewide studies of treatment outcomes of alcohol and drug abuse services, this paper concludes that a strict adherence to an abstinence-only model of success, rigidly adopted by many in the treatment industry is counterproductive. Multiple measures of success are essential to fully understand and assess a changing model of intervention in the chemical dependency field.


The Relationships Among Competitiveness, Age And Ability In Distance Runners, Robert C. Eklund, Jeffrey J. Martin, Alan L. Smith Dec 1994

The Relationships Among Competitiveness, Age And Ability In Distance Runners, Robert C. Eklund, Jeffrey J. Martin, Alan L. Smith

Kinesiology, Health and Sport Studies

The purpose of this study was to examine relationships suggested by general achievement motivation literature and the popular literature in sport using the Sport Orientation Questionnaire (SOQ). The current study examined if faster runners are more competitive than slower runners, if older athletes were less competitive than younger athletes, and if faster runners were more goal oriented than slower runners. Distance runners (n=80), ranging from 10 to 61 years old completed race packets containing a cover letter, consent forms, the SOQ and a demographic questionnaire. Runners averaged 32.9 years of age and reported levels of competitiveness and goal ...


Sidedness Of H+ Action On K-Cl Cotransport In Dids Ph(I)-Clamped Low K Sheep Red Blood Cells With Reduced Mg(I), Peter K. Lauf, Norma C. Adragna Dec 1994

Sidedness Of H+ Action On K-Cl Cotransport In Dids Ph(I)-Clamped Low K Sheep Red Blood Cells With Reduced Mg(I), Peter K. Lauf, Norma C. Adragna

Pharmacology and Toxicology Faculty Publications

No abstract provided.


Overview And New Technology In Cyclodestructive Procedures, Jill Fishbaugh Dec 1994

Overview And New Technology In Cyclodestructive Procedures, Jill Fishbaugh

Department of Ophthalmology and Visual Sciences Publications

When all medical and surgical measures have failed to lower intraocular pressure (IOP) in patients with severe and uncontrolled glaucoma, ophthalmologists must turn to cyclodestructive procedures to decrease aqueous production. Several months ago, the FDA (Food and Drug Administration) came through with an approval for a new technology in cyclophotocoagulation therapy. The IRIS Medical G-Probe™ used in conjunction with a semiconductor diode laser may be the superior alternative to previously available cyclodestructive techniques for which ophthalmologists have been searching.


The Effects Of Low Frequency Electrical Stimulation On Muscular Strength And Endurance In Individuals With Multiple Sclerosis, Lisa L. Oglesby Dec 1994

The Effects Of Low Frequency Electrical Stimulation On Muscular Strength And Endurance In Individuals With Multiple Sclerosis, Lisa L. Oglesby

Doctoral Dissertations

The purpose of this study was to investigate the results of a practical application of a low frequency electrical stimulation (LFES) program of 8 pps to individuals with multiple sclerosis. Previous research has shown that such a program induces a conversion of fast twitch muscle properties to those of slow twitch. Electrical stimulation was applied for 3 hours per day, 6 days a week, for 6 weeks, to the quadriceps femoris muscle of nine subjects with multiple sclerosis. Pre and post measurements of average peak torque, mean force, and fatigue slope were taken as indices of the muscle's strength ...


A Comparison Of Six Personality Factors Between Professional, College, And High School Basketball Players, William G. Bowe Jr. Dec 1994

A Comparison Of Six Personality Factors Between Professional, College, And High School Basketball Players, William G. Bowe Jr.

Kinesiology, Sport Studies, and Physical Education Master’s Theses

This investigation was concerned with comparing six personality factors among professional, college, and high school basketball players. The different factors measured include competitive trait anxiety, trait self-confidence, concentration, mental preparation skills, achievement motivation levels, and leadership skills. A self-evaluation questionnaire was administered to five basketball teams (two high school, two college, and one professional). Each subject's questionnaire was scored and a Mental Toughness Profile for each athlete was developed. The purpose of this investigation was to determine if there is a difference in personality factors among basketball players at the professional, collegiate, and high school levels.

An Analysis Of ...


Ileocaecal Tuberculosis: A Pictorial Essay Of Various Appearances On Small Bowel Enema, M Y. Shaikh, T U. Haq, U M. Islam, Z A. Khan Dec 1994

Ileocaecal Tuberculosis: A Pictorial Essay Of Various Appearances On Small Bowel Enema, M Y. Shaikh, T U. Haq, U M. Islam, Z A. Khan

Department of Radiology

No abstract provided.


Non-Opsonic Attachment Of Bordetella Bronchiseptica Mediated By Cd11/Cd18 And Cell Surface Carbohydrates, Karen B. Register, Mark R. Ackermann, Marcus E. Kehrli Jr. Dec 1994

Non-Opsonic Attachment Of Bordetella Bronchiseptica Mediated By Cd11/Cd18 And Cell Surface Carbohydrates, Karen B. Register, Mark R. Ackermann, Marcus E. Kehrli Jr.

Mark R. Ackermann

Porcine atrophic rhinitis associated with Bordetella bronchiseptica is characterized by a severe inflammatory response in the mucosa of the nasal turbinates. Initial infiltrates of polymorphonuclear leukocytes (PMN) are followed by accumulations of mononuclear cells. In this report, we have investigated the interaction between porcine PMN and B. bronchiseptica. PMN incubated in PBS with a fluorescently labeled hemagglutinating porcine isolate, but not a non-hemagglutinating variant, had high levels of cell-associated fluorescence as determined by flow cytometry. Light microscopy indicated that most cell-associated bacteria were ingested. Transmission electron microscopy confirmed the presence of intracellular bacteria, which were contained within membrane-bound phagosomes. A ...


Destructive Hostility: The Jeffrey Dahmer Case: A Psychiatric And Forensic Study Of A Serial Killer, Jeffrey Jentzen, George Palermo, L. Thomas Johnson, Khang-Cheng Ho, K. Alan Stormo, John Teggatz Dec 1994

Destructive Hostility: The Jeffrey Dahmer Case: A Psychiatric And Forensic Study Of A Serial Killer, Jeffrey Jentzen, George Palermo, L. Thomas Johnson, Khang-Cheng Ho, K. Alan Stormo, John Teggatz

School of Dentistry Faculty Research and Publications

We were involved as forensic experts in the case of the serial killer Jeffrey Dahmer. We discuss the scene and victim autopsy findings, with a brief consideration of the basic emotion of hostility. These findings support the thesis that at the basis of this serial killer's behavior were primary unconscious feelings of hate that he had channeled into a sadistic programmed destruction of 17 young men. The interview of the serial killer, the photographic scene documentation, and the autopsy findings stress the ambivalent homosexuality of the killer, his sexual sadism, his obsessive fetishism, and his possible cannibalism and necrophilia.


Signal Transduction By Tumor Necrosis Factor Mediated By Jnk Protein Kinases, Hayla Karen Sluss, Tamera Barrett, Benoit Derijard, Roger J. Davis Dec 1994

Signal Transduction By Tumor Necrosis Factor Mediated By Jnk Protein Kinases, Hayla Karen Sluss, Tamera Barrett, Benoit Derijard, Roger J. Davis

Open Access Articles

JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1. Here we describe the molecular cloning of a second member of the JNK group, the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation. Furthermore, JNK protein kinase activation is observed in cells treated with tumor necrosis factor. Although both JNK isoforms phosphorylate the NH2-terminal activation domain of the transcription factor c-Jun, the activity of JNK2 was approximately ...


Regulation Of The Human Cellular Glutathione Peroxidase Gene During In Vitro Myeloid And Monocytic Differentiation, Qichang Shen, Sunil Chada, Constance Whitney, Peter E. Newburger Dec 1994

Regulation Of The Human Cellular Glutathione Peroxidase Gene During In Vitro Myeloid And Monocytic Differentiation, Qichang Shen, Sunil Chada, Constance Whitney, Peter E. Newburger

Open Access Articles

We have used the HL-60 and PLB-985 myeloid leukemia cell lines to examine the regulation of expression of the important intracellular antioxidant enzyme, glutathione peroxidase (GSH-Px), during phagocytic cell differentiation in vitro. Induction of differentiation along the monocytic pathway by phorbol ester results in an approximately twofold rise in enzyme activity and a parallel increase in the rate of 75Se incorporation into immunoprecipitable GSH-Px protein. Induction along the granulocytic pathway by dimethyl formamide (DMF) results in similar changes in steady-state enzyme levels and rates of GSH-Px protein synthesis. Steady-state levels of GSH-Px gene transcripts also increase more than twofold, approximately ...


Knowledge, Attitudes, Behavior, And Decision-Making Orientation Relating To Cardiovascular Health In High School Students, Patricia E. Bromfield Dec 1994

Knowledge, Attitudes, Behavior, And Decision-Making Orientation Relating To Cardiovascular Health In High School Students, Patricia E. Bromfield

Master's Theses

Planning for cardiovascular health (CVH) education for adolescents

requires an awareness of students' cardiovascular knowledge and attitudes

toward "heart healthy" behavior, and an understanding of the dynamics

of health-related decision-making in this age group. This study attempted

to measure these parameters using an 81-item survey administered to 93

9th graders (45 females, 46 males, 2 undisclosed) and 107 12th graders (57

females, 47 males, 3 undisclosed). The survey was administered m a

suburban high school in southwest Michigan in the spring of 1994.

Results were evaluated descriptively for knowledge, attitudes, and

behavior, and for differences across grade and gender. ANOVA ...


Chronic Granulomatous Disease And Glutathione Peroxidase Deficiency, Revisited, Peter E. Newburger, Stephen E. Malawista, Mary C. Dinauer, Terri Gelbart, Richard C. Woodman, Sunil Chada, Qichang Shen, Gretchen Van Blaricom, Paul G. Quie, John T. Curnutte Dec 1994

Chronic Granulomatous Disease And Glutathione Peroxidase Deficiency, Revisited, Peter E. Newburger, Stephen E. Malawista, Mary C. Dinauer, Terri Gelbart, Richard C. Woodman, Sunil Chada, Qichang Shen, Gretchen Van Blaricom, Paul G. Quie, John T. Curnutte

Open Access Articles

We have restudied two kindreds that formed the basis of the original report of autosomal recessive chronic granulomatous disease (CGD) associated with leukocyte glutathione peroxidase deficiency. Case 1 from the original study and the surviving brother of the originally reported case 2 both have severe CGD, with no detectable respiratory burst activity in purified intact neutrophils. However, their leukocytes exhibit normal glutathione peroxidase enzyme activity and gene expression. Examination of phagocyte nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase components known to be defective in CGD reveals no detectable cytochrome b558 nor any membrane activity in a cell-free NADPH oxidase assay system ...