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Articles 31 - 60 of 150
Full-Text Articles in Medicine and Health Sciences
Detection Of Necrotizing Hepatopancreatitis (Nhp) In Wild Shrimp From Laguna Madre, Mexico By A Multiplex Polymerase Chain Reaction, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Roberto Pérez-Castañeda, Rafael Orta-Rodriguez
Detection Of Necrotizing Hepatopancreatitis (Nhp) In Wild Shrimp From Laguna Madre, Mexico By A Multiplex Polymerase Chain Reaction, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Roberto Pérez-Castañeda, Rafael Orta-Rodriguez
The Thai Journal of Veterinary Medicine
The Laguna Madre in Mexico is a nursery, growth, and refuge area for shrimp and other wildlife species. These organisms support an important fishery for local industry (third place in Mexico), which may suffer diseases that are few studied. Wild shrimp were randomly collected from two sampling stations (Carrizal and Carbonera) once a month for one year (September 2007 to September 2008) to screen for virus and bacteria. The shrimp were taxonomically identified, organized in pools of three organisms per species and analyzed for the presence of WSSV, IHHNV, NHP and HPV by a commercial multiplex PCR kit DiagXotics Inc. …
Comparison Of The Efficacy Of Enrofloxacin Against Escherichia Coli Or Pasteurella Multocida Infection In Chickens, Visut Rawiwet, Piyarat Chansiripornchai, Niwat Chansiripornchai
Comparison Of The Efficacy Of Enrofloxacin Against Escherichia Coli Or Pasteurella Multocida Infection In Chickens, Visut Rawiwet, Piyarat Chansiripornchai, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
This study compared the efficacy of enrofloxacin, given immediately or 6 hrs after infection against Escherichia coli or Pasteurella multocida. In the first experiment, thirty, 12-week-old male layer chickens were divided into 3 groups of 10 each. Chickens in group 1, 2 and 3 were infected with E. coli serotype O78 by left thoracic air sac injection. Group 1 was given enrofloxacin (10 mg/kg), dissolved in drinking water immediately receiving E. coli. Group 2 was given enrofloxacin 6 hrs after receiving E. coli. Group 3 served as a positive control. In the second experiment, twenty, 16-week-old male layer chickens were …
Forensic Veterinary Medicine, Nantarika Chansue
Forensic Veterinary Medicine, Nantarika Chansue
The Thai Journal of Veterinary Medicine
No abstract provided.
The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen
The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
A hundred-eighty Escherichia coli isolates from swine were tested for minimal inhibitory concentrations (MICs) of 9 antimicrobials and two heavy metals copper sulfate and zinc chloride. All the E. coli isolates were resistant to at least one antibiotic and 175 (97.2%) isolates were multi-drug resistant. Forty antibiotic resistance patterns were identified, of which the most common pattern was AMP-CHP-CIP-ERY-GEN-STR-SUL-TET-TRI (13.3%). All the isolates formed one large population of susceptibility to copper sulfate and zinc chloride at the concentrations of 6.4-12.8 mM and 3.2-6.4 mM, respectively. Exposure to copper sulfate and zinc chloride at sublethal concentration did not change the susceptibility. …
Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn
Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn
The Thai Journal of Veterinary Medicine
No abstract provided.
Evaluation Of Serum Nt-Probnp Levels In Dogs With Respiratory Distress Associated With Heart Disease And Respiratory Disease, Amornrate Sastravaha, Santi Kaewmokul, Pareeya Udomkusonsri, Naris Thengchaisri
Evaluation Of Serum Nt-Probnp Levels In Dogs With Respiratory Distress Associated With Heart Disease And Respiratory Disease, Amornrate Sastravaha, Santi Kaewmokul, Pareeya Udomkusonsri, Naris Thengchaisri
The Thai Journal of Veterinary Medicine
The association between severity of heart disease and serum N-terminal prohormone B-type natriuretic peptide (NT-proBNP) was determined in dogs presented with respiratory distress. Four groups of dogs were divided into healthy control (n=11), heart failure (n=58), heart disease without heart failure (n=17) and respiratory disease (n=20). Serum NT-proBNP concentrations were measured with an ELISA designed for using in dogs. Dirofilaria antigen tests were detected in dogs with heart disease or heart failure. Serum NT-proBNP concentrations of dogs with heart failure (2,977±184 pmol/l) were significantly higher than dogs with heart disease (611±46 pmol/l), respiratory disease (583±52 pmol/l) or physically healthy (426±32 …
Ultrasound Diagnosis, Phiwipha Kamonrat
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Myxomatous Degenerative Mitral Valve Disease: An Update, Sirilak Disatian
Myxomatous Degenerative Mitral Valve Disease: An Update, Sirilak Disatian
The Thai Journal of Veterinary Medicine
Myxomatous degenerative mitral valve disease (MVD) is the most common cardiac disease in dogs.Pathological changes of MVD include thickened redundant leaflets causing improper coaptation of leaflets, resultingin mitral valve regurgitation. The etiology of this disease is currently unknown. Thus, no drugs or treatments areavailable to slow down the disease progression. This article was written to summarize an update of canine MVD concerning clinical approach, etiology, and treatment.
Comparison Of Carprofen, Vedaprofen And Tepoxalin For Postoperative Analgesia And Serum Pge2 Level In Dogs After Ovariohysterectomy, Naris Thengchaisri, Panpicha Sattasathuchana, Sirirat Niyom, Wannasit Chantornvong
Comparison Of Carprofen, Vedaprofen And Tepoxalin For Postoperative Analgesia And Serum Pge2 Level In Dogs After Ovariohysterectomy, Naris Thengchaisri, Panpicha Sattasathuchana, Sirirat Niyom, Wannasit Chantornvong
The Thai Journal of Veterinary Medicine
Comparison of analgesic efficacy of 3 non-steroidal analgesic drugs (carprofen, vedaprofen and tepoxalin) were performed in 40 dogs after ovariohysterectomy. Oral administration of placebo (sugar pill), carprofen (4.4 mg/kg/day), vedaprofen (0.5 mg/kg/day), or tepoxalin (20 mg/kg/day) for 3 days were performed in dogs recovering from ovariohysterectomy. Numerical pain scores (descriptive and composite pain scores) were measured in blinded fashion by three investigators at 0, 12, 24, 36, 48 and 60 hrs following the recovery. The average descriptive and composite pain scores of dogs treated with carprofen or tepoxalin were significantly lower than that of the control group (p<0.05), however, no significance difference was found between the vedaprofen-treated and control groups (p>0.05). Serum …
Lateral Patellar Luxation In Three Pomeranian Dogs: A Case Report, Chalika Wangdee, Pinrath Torwattanachai
Lateral Patellar Luxation In Three Pomeranian Dogs: A Case Report, Chalika Wangdee, Pinrath Torwattanachai
The Thai Journal of Veterinary Medicine
Three Pomeranians with bilateral lateral patellar luxation (LPL) were present at the Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University. Trochlear block recession was performed on all the dogs. The additional procedures including lateral retinacular desmotomy, tibial derotation, imbrication of medial retinaculum, and/or proximal tube realignment. Medial transposition of the tibial tuberosity was additionally done in one dog. Recurrent right LPL was found and corrected in one leg of the second dog. Following at least 3 months, all three dogs walked, jumped, and put weight normally on the operated legs after the operation.
Intra-Uterine And Deep Intra-Uterine Insemination Using Cryopreserved Boar Semen In Spontaneously-Ovulating Sows, Kakanang Buranaamnuay, Termpong Wongtawan, Sutthatip Masuwatana, Padet Tummaruk, Mongkol Techakumphu
Intra-Uterine And Deep Intra-Uterine Insemination Using Cryopreserved Boar Semen In Spontaneously-Ovulating Sows, Kakanang Buranaamnuay, Termpong Wongtawan, Sutthatip Masuwatana, Padet Tummaruk, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The present study was performed to investigate the in vivo fertility of frozen-thawed (FT) boar semen after intra-uterine (IUI) and deep intra-uterine insemination (DIUI) in spontaneously-ovulating sows. A total of 48 weaned sows were included. The sows were divided into three groups, i.e. natural mating (NM) (n=30), IUI (n=9) and DIUI (n=9). In the IUI and DIUI groups, the sows were inseminated twice, at 24 and 36 h after the detection of oestrous by IUI with 2x109 spermatozoa/dose or DIUI with 1x109 spermatozoa/dose. Transrectal ultrasonography was used to determine the time of ovulation after insemination. The results revealed that the …
Clinical, Radiographic And Pathological Features Of Persistent Gastroesophageal Intussusception In An Adult Dog: A Case Report, Theerayuth Kaewamatawong, Wijit Banlunara, Varaporn Wangrattanaparanee, Srireepong Kiertkrittikhoon
Clinical, Radiographic And Pathological Features Of Persistent Gastroesophageal Intussusception In An Adult Dog: A Case Report, Theerayuth Kaewamatawong, Wijit Banlunara, Varaporn Wangrattanaparanee, Srireepong Kiertkrittikhoon
The Thai Journal of Veterinary Medicine
The aim of this report is to describe the clinical, radiographic and pathological appearances of a persistent gastroesophageal intussusception in associated with idiopathic egaesophagus in an adult dog. A 6-years old, male Golden Retriever was referred for evaluation of chronic regurgitation and vomiting. Radiographic diagnosis of persistent gastroesophageal intussusception (GEI) with megaesophagus was made. Survey and contrast radiography evidenced a distension of the esophageal medial and caudal portions due to a dense soft-tissue mass and the barium cloud not pass to abdominal portion, where outlined the striations of muscle of the distal esophagus around the mass.Necropsy findings revealed a completed …
Ophthalmology Snapshot, Nalinee Tuntivanich
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Effect Of Water Temperature On Hematology And Virulence Of Aeromonas Hydrophila In Hybrid Catfish (Clarias Gariepinus Burchell X C. Macrocephalus Gunther), Weena Koeypudsa, Malinee Jongjareanjai
Effect Of Water Temperature On Hematology And Virulence Of Aeromonas Hydrophila In Hybrid Catfish (Clarias Gariepinus Burchell X C. Macrocephalus Gunther), Weena Koeypudsa, Malinee Jongjareanjai
The Thai Journal of Veterinary Medicine
Water temperature was set up at 2 levels; high (Ht, 29.5±0.5oC) and low temperature (Lt, 19.5±0.5oC). Experimental catfish body weight and total length were 12.07±1.41 g and 11.57±0.62 cm respectively. Catfish were divided into 4 groups: high temperature with 0.1% sodium chloride (NaCl) (HtWs), high temperature without 0.1% NaCl (HtW/s), low temperature with 0.1% NaCl (LtWs), and low temperature without 0.1% NaCl (LtW/s). Catfish hematocrit (Hct) and clinical chemistry including serum glutamic oxaloacetic transminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), and creatinine (Cr) were measured. The results showed that water temperature, Ht (HtWs …
Ecg Quiz, Chollada Buranakarl, Monkon Trisiriroj
Ecg Quiz, Chollada Buranakarl, Monkon Trisiriroj
The Thai Journal of Veterinary Medicine
No abstract provided.
Roadmap Of Torture: Captive Thai Elephant And The Anecdote, Sumolya Kanchanapangka
Roadmap Of Torture: Captive Thai Elephant And The Anecdote, Sumolya Kanchanapangka
The Thai Journal of Veterinary Medicine
No abstract provided.
Canine Heartworm (Dirofilaria Immitis) Infection And Immunoglobulin G Antibodies Against Wolbachia (Rickettsiales: Rickettsiaceae) In Stray Dogs In Bangkok, Thailand, Sonthaya Tiawsirisup, Teerat Thanapaisarnkit, Euapong Varatorn, Taddao Apichonpongsa, Nattapon Bumpenkiattikun, Sirirat Rattanapuchpong, Sudchit Chungpiwat, Vivornpun Sanprasert, Surang Nuchprayoon
Canine Heartworm (Dirofilaria Immitis) Infection And Immunoglobulin G Antibodies Against Wolbachia (Rickettsiales: Rickettsiaceae) In Stray Dogs In Bangkok, Thailand, Sonthaya Tiawsirisup, Teerat Thanapaisarnkit, Euapong Varatorn, Taddao Apichonpongsa, Nattapon Bumpenkiattikun, Sirirat Rattanapuchpong, Sudchit Chungpiwat, Vivornpun Sanprasert, Surang Nuchprayoon
The Thai Journal of Veterinary Medicine
Canine heartworm (Dirofilaria immitis) infection in stray dogs was studied by using parasitological (fresh blood smear, thin blood smear, thick blood smear, and modified Knott’s test) and serological methods. Blood samples were collected from stray dogs in Bangkok metropolitan area during 2006 and 2008. There were 10% (50/500) of stray dogs infected with D. immitis, which was indicated by using parasitological methods. Microfilaria levels were evaluated from 36 infected dogs and the range of microfilarial levels were between 17 and 78,417 microfilariae per milliliter of blood. Fifty serum samples from D. immitis infected dogs and 310 serum samples from non-infected …
The Sperm Dna Damage After Cryopreservation Of Boar Semen In Relation To Post-Thawed Semen Qualities, Antioxidant Supplementation And Boars Effects, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk
The Sperm Dna Damage After Cryopreservation Of Boar Semen In Relation To Post-Thawed Semen Qualities, Antioxidant Supplementation And Boars Effects, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The objectives of the present study were to evaluate the damage of DNA of the frozen-thawed (FT) boar spermatozoa and to investigate the effect of various concentrations of L-cysteine supplementation on the sperm DNA damage. A total of 104 cryopreserved semen samples from twenty-six ejaculates of 16 proven boars were analyzed. Of these samples, each semen sample contained a different concentration of L-cysteine i.e., 0 (n=41), 5 (n=41), 10 (n=11) and 15 (n=11) mM. All of the semen samples were cryopreserved by controlled-rate freezer. The semen was thawed at 50oC for 12 sec and the damage to the sperm DNA …
Effects Of Cooling And Recombinant Bovine Somatotropin Supplementation On Body Fluids, Mammary Blood Flow, And Nutrients Uptake By The Mammary Gland In Different Stages Of Lactation Of Crossbred Holstein Cattle, Siravit Sitprija, Somchai Chanpongsang, Narongsak Chaiyabutr
Effects Of Cooling And Recombinant Bovine Somatotropin Supplementation On Body Fluids, Mammary Blood Flow, And Nutrients Uptake By The Mammary Gland In Different Stages Of Lactation Of Crossbred Holstein Cattle, Siravit Sitprija, Somchai Chanpongsang, Narongsak Chaiyabutr
The Thai Journal of Veterinary Medicine
and in shaded with misty-fan cooling (MFC) as cooled cows. The cows were treated with recombinant bovine somatotropin (rbST) in early, mid and late lactation with three consecutive injections of rbST 500 mg of rbST (POSILAC) in every 14 days. During the study, ambient temperature at the hottest period daily (1400 hr) in the MFC barn was significantly lower, while relative humidity was higher than that of the NS barn. The temperature humidity index (THI) in both barns ranged from 80.7-85.5 throughout the periods of study. Cows in the MFC barn showed a lower rectal temperature and respiration rate as …
Characterization And Probiotic Properties Of Bacillus Strains Isolated From Broiler, Neungnut Chaiyawan, Punnathorn Taveeteptaikul, Bhusita Wannissorn, Supatjaree Ruengsomwong, Prapaipat Klungsupya, Wannaluk Buaban, Pariyaporn Itsaranuwat
Characterization And Probiotic Properties Of Bacillus Strains Isolated From Broiler, Neungnut Chaiyawan, Punnathorn Taveeteptaikul, Bhusita Wannissorn, Supatjaree Ruengsomwong, Prapaipat Klungsupya, Wannaluk Buaban, Pariyaporn Itsaranuwat
The Thai Journal of Veterinary Medicine
In order to isolate local strain of spore-forming bacteria having probiotic properties, 164 isolates of the sporeforming bacteria were isolated from 152 samples including duodenum, jejunum, ileum and cecum collected from 38 local backyard chickens raised in the northern part of Thailand. These isolates were preliminarily screened for antibiotic susceptibility and antimicrobial acitivity. Only antibiotic-susceptible isolates with antimicrobial activity were further characterized for the ability to resist certain harsh environments. Identification of the potential isolates was carried out by morphological and biochemical studies, followed by analysis of 16S rDNA sequence. Of these isolates, only one strain designated T3-1 showed antimicrobial …
What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph
What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph
The Thai Journal of Veterinary Medicine
No abstract provided.
Effect Of Using Supernatant For Post-Thawing Solution And Semen Extender Prior To Insemination On Sow Reproductive Performance, Cholthida Kasetrtut, Kampon Kaeoket
Effect Of Using Supernatant For Post-Thawing Solution And Semen Extender Prior To Insemination On Sow Reproductive Performance, Cholthida Kasetrtut, Kampon Kaeoket
The Thai Journal of Veterinary Medicine
The objective of the present study was to determine the effects of using supernatant (seminal plasma:extender, 50:50 v/v) as post-thawing solution and semen extender on sow fertility. Fifteen sows were allocated into the following experimental groups: Groups A (control), sows (n=5) were inseminated with fresh semen, using a dose of 4x109 spermatozoa in 60 ml of Modena™ extender; Group B, sows (n=5) were inseminated with frozen semen (supplemented with 10 mM of L-cysteine), using a dose of 2x109 spermatozoa in 60 ml of supernatant (50% v/v of seminal plasma plus ModenaTM extender); Group C, sows (n=5) were inseminated with frozen …
Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Sirilak Disatian, Winai Chansaisakorn, Piyasiri Glangosol
Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Sirilak Disatian, Winai Chansaisakorn, Piyasiri Glangosol
The Thai Journal of Veterinary Medicine
No abstract provided.
Cloned Asian Elephant (Elephas Maximus) Embryos Reconstructed From Rabbit Recipient Oocytes, Mongkol Techakumphu, Ruttachuk Rungsiwiwut, Pranee Numchaisrika, Ampika Thongphakdee
Cloned Asian Elephant (Elephas Maximus) Embryos Reconstructed From Rabbit Recipient Oocytes, Mongkol Techakumphu, Ruttachuk Rungsiwiwut, Pranee Numchaisrika, Ampika Thongphakdee
The Thai Journal of Veterinary Medicine
The information about reproductive biology, especially embryo development of Asian elephant (Elephas maximus) generated from naturally fertilization is lacking. In the present study, somatic cell nuclear transfer (SCNT) was applied as an alternative way to produce the elephant embryos. The fibroblasts derived from ear skin of Asian elephant and rabbit were used as the donor cells and rabbit oocytes were used as the recipient cytoplasm. The objectives of the study were 1) to find the optimal conditions for fusion and activation by using electrical pulses (experiment I) and 2) to investigate the in vitro development of cloned Asian elephant in …
Efficacy Of Infectious Bursal Disease Vaccine In Broiler Chickens Receiving Different Vaccination Programs, Chatchai Sarachai, Niwat Chansiripornchai, Jiroj Sasipreeyajan
Efficacy Of Infectious Bursal Disease Vaccine In Broiler Chickens Receiving Different Vaccination Programs, Chatchai Sarachai, Niwat Chansiripornchai, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
The objective of this study was to evaluate the efficacy of 3 infectious bursal disease vaccination programs in commercial broiler chickens. One hundred and forty, 1-day-old female broiler chickens were divided into 4 groups of 35 chickens each. Groups 1-3 were vaccinated with infectious bursal disease (IBD) vaccine via oral administration at 1, 1 and 16, and 16-day-old, respectively. Group 4 served as a positive control group. Body weight, sera collection, bursa: body weight (B:BW) ratios and histopathological lesion scores (HLS) were evaluated at 16, 30 and 40-day-old. All the chickens in groups 1 to 4 were challenged with IBD …
Studies Of Oestrogen And Progesterone Receptors In Reproductive Organs Of Prepubertal Gilts With Reproductive Disturbance, Sayamon Srisuwatanasagul, Padet Tummaruk, Annop Kunavongkrit
Studies Of Oestrogen And Progesterone Receptors In Reproductive Organs Of Prepubertal Gilts With Reproductive Disturbance, Sayamon Srisuwatanasagul, Padet Tummaruk, Annop Kunavongkrit
The Thai Journal of Veterinary Medicine
The present study aims to study the localization of steroid receptor, oestrogen receptor alpha and progesterone receptor by immunohistochemical technique in the reproductive organs of prepubertal culling gilts with reproductive disturbance. The genital organs from 20 culled gilts before puberty were collected. Historical data, reasons of culling and the macroscopic lesions of the reproductive tracts were recorded. The results of immunohistochemistry revealed that the expression of ER and PR was lower in prepubertal gilts with reproductive lesion compared to normal gilts. However, a significant difference was found only in some compartments of the cervix and uterus. In the cervix, a …
Unilateral Polydactylism In A German Holstein-Friesian Calf - A Case Report, Bianca Carstanjen, Juliane Pennecke, Sara Boehart, Kerstin E. Müller
Unilateral Polydactylism In A German Holstein-Friesian Calf - A Case Report, Bianca Carstanjen, Juliane Pennecke, Sara Boehart, Kerstin E. Müller
The Thai Journal of Veterinary Medicine
This case report describes the diagnosis and surgical treatment of unilateral polydactylism of unknown origin in a calf. A six-week-old female German Holstein-Friesian calf was referred to the clinic with a hyperflexion of the fetlock and carpal joints of the right forelimb, associated with two surplus digits in the same leg, which was confirmed by radiographic examination. Surgery consisted of an osteotomy of the second metacarpal bone, a disarticulation in the antebrachiocarpal joint of the additional limb combined with an amputation of both odd digits. The follow-up of 12 months revealed a functionally sound heifer showing a good cosmetic outcome.
Morphological Changes And Infiltration Of Immune Cells In The Endometrium Of Anoestrus Gilt In Relation To The Ovarian Appearance And Serum Progesterone, Yuttapol Teamsuwan, Kampon Kaeoket, Paisan Tienthai, Padet Tummaruk
Morphological Changes And Infiltration Of Immune Cells In The Endometrium Of Anoestrus Gilt In Relation To The Ovarian Appearance And Serum Progesterone, Yuttapol Teamsuwan, Kampon Kaeoket, Paisan Tienthai, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigates morphological changes and distribution of the leukocyte subpopulation in the endometrium of anoestrus gilts in relation to reproductive cycles and serum progesterone (P4). Selected genital organs from 30 gilts culled due to anoestrus were examined. The genital organs were classified according to the ovarian appearance into 3 groups, i.e. inactive (n = 10); follicular (n = 10); and luteal phase (n = 10). Blood samples were collected prior to slaughter to determine serum P4. Seven tissue samples were randomly collected from the uteri of the gilts and were examined for histological structures, i.e. epithelial types and …
The Use Of Computer-Assisted Sperm Analysis For Discriminating Series Of Motility Pattern Of Frozen-Thawed Boar Semen, Chanyuth Tretipskul, Kakanang Buranaamnuay, Seri Koonjaenak, Padet Tummaruk, Mongkol Techakumphu
The Use Of Computer-Assisted Sperm Analysis For Discriminating Series Of Motility Pattern Of Frozen-Thawed Boar Semen, Chanyuth Tretipskul, Kakanang Buranaamnuay, Seri Koonjaenak, Padet Tummaruk, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Computer-assisted sperm analysis (CASA) was initiated to reduce subjective bias on the motility assessment and to discriminate a series of motility patterns of boar semen. The objectives of this present study were to evaluate the motility pattern of frozen-thawed boar semen at 0 and 60 min after thawing using CASA. Forty one ejaculates from Landrace (n=14), Yorkshire (n=12) and Duroc (n=15) boars were cryopreserved and included in the experiment. The semen was thawed at 37°C for 30 sec. The post-thawed sperm qualities including subjective motility, plasma membrane integrity and motility pattern were determined immediately after thawing (T0) and at 60 …
Avian Influenza Virus (H5n1) Inactivation By Binary Ethylenimine, Chatchai Sarachai, Jiroj Sasipreeyajan, Niwat Chansiripornchai
Avian Influenza Virus (H5n1) Inactivation By Binary Ethylenimine, Chatchai Sarachai, Jiroj Sasipreeyajan, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
Avian influenza virus (AIV) subtype H5N1 is known as a highly pathogenic agent for man and various kinds of animals, especially avian species. Herein, AIV H5N1 was inactivated by binary ethylenimine (BEI), an aziridine compound, produced from 2-bromoethylamine hydrobromide. BEI was used at concentrations of 0.001 and 0.01 M for AIV inactivation. At the lower concentration (0.001 M), AIV could be inactivated within 24 hr. At the higher concentration (0.01 M), AIV could be inactivated within 6 hr. After inactivation, no hemagglutination activity had been found meaning that AIV had been completely inactivated.