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Full-Text Articles in Medicine and Health Sciences
Prevalence Of The Cellular And Molecular Antimicrobial Resistance Against E. Coli Isolated From Thai Broilers, Sarawoot Mooljuntee, Piyarat Chansiripornchai, Niwat Chansiripornchai
Prevalence Of The Cellular And Molecular Antimicrobial Resistance Against E. Coli Isolated From Thai Broilers, Sarawoot Mooljuntee, Piyarat Chansiripornchai, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
The antimicrobial resistant characteristics both of the cellular and molecular levels of Escherichia coli in broilers were performed by disk diffusion test and polymerase chain reaction technique. Specific primers for the aadA, aac(3)-IV, cmlA, cat1, tetA, sul1, SHV, CITM, ereA and dhfrV gene were used to detect resistance to gentamicin, chloramphenicol, tetracycline, cephalothin, ampicillin, erythromycin, sulfonamide+trimetroprim. The 30 samples of E. coli were found to be 100% antimicrobial disk resistant to tetracycline, ampicillin and erythromycin and revealed a 90%, 93.3% and 73.3% resistant gene to tetA, CITM and ereA, respectively. While resistance to 73.3% cephalothin and 26.7% sulfonamide+trimethoprim was revealed …
The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen
The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
A hundred-eighty Escherichia coli isolates from swine were tested for minimal inhibitory concentrations (MICs) of 9 antimicrobials and two heavy metals copper sulfate and zinc chloride. All the E. coli isolates were resistant to at least one antibiotic and 175 (97.2%) isolates were multi-drug resistant. Forty antibiotic resistance patterns were identified, of which the most common pattern was AMP-CHP-CIP-ERY-GEN-STR-SUL-TET-TRI (13.3%). All the isolates formed one large population of susceptibility to copper sulfate and zinc chloride at the concentrations of 6.4-12.8 mM and 3.2-6.4 mM, respectively. Exposure to copper sulfate and zinc chloride at sublethal concentration did not change the susceptibility. …