Agronomy and Crop Sciences Commons^™

Next-Generation Technologies Unlock New Possibilities To Track Rangeland Productivity And Quantify Multi-Scale Conservation Outcomes, Caleb P. Roberts, David E. Naugle, Brady W. Allred, Victoria M. Donovan, Dillon T. Fogarty, Matthew O. Jones, Jeremy D. Maestas, Andrew C. Olsen, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

Historically, relying on plot-level inventories impeded our ability to quantify large-scale change in plant biomass, a key indicator of conservation practice outcomes in rangeland systems. Recent technological advances enable assessment at scales appropriate to inform management by providing spatially comprehensive estimates of productivity that are partitioned by plant functional group across all contiguous US rangelands. We partnered with the Sage Grouse and Lesser Prairie-Chicken Initiatives and the Nebraska Natural Legacy Project to demonstrate the ability of these new datasets to quantify multi-scale changes and heterogeneity in plant biomass following mechanical tree removal, prescribed fire, and prescribed grazing. In Oregon’s sagebrush …

Cytogenetic And Genomic Characterization Of A Novel Tall Wheatgrass‑Derived Fhb7 Allele Integrated Into Wheat B Genome, Wei Zhang, Tatiana Danilova, Mingyi Zhang, Shuangfeng Ren, Xianwen Zhu, Qijun Zhang, Shaobin Zhong, Linda Dykes, Jason Fiedler, Steven Xu, Katherine Frels, Stephen Wegulo, Jeffrey Boehm Jr., Xiwen Cai

Department of Agronomy and Horticulture: Faculty Publications

A novel tall wheatgrass-derived (Thinopyrum elongatum, genome EE) Fhb7 allele, designated Fhb7^The2, was identified and integrated into the wheat B genome through a small 7B–7E translocation (7BS·7BL–7EL) involving the terminal regions of the long arms. Fhb7^The2 conditions significant Type II resistance to Fusarium head blight (FHB) in wheat. Integration of Fhb7^The2 into the wheat B genome makes this wild species-derived FHB resistance gene usable for breeding in both common and durum wheat. By contrast, other Fhb7 introgression lines involving wheat chromosome 7D can be utilized only in common wheat breeding programs, not in durum …

How Digital Is Agriculture In A Subset Of Countries From South America? Adoption And Limitations, Laila A. Puntel, É. L. Bolfe, R. J. M. Melchiori, R. Ortega, G. Tiscornia, A. Roel, F. Scaramuzza, S. Best, A. G. Berger, D. S. S. Hansel, D. Palacios Durán, G. R. Balboa

Department of Agronomy and Horticulture: Faculty Publications

Digital agriculture (DA) can contribute solutions to meet an increase in healthy, nutritious, and affordable food demands in an efficient and sustainable way. South America (SA) is one of the main grain and protein producers in the world but the status of DA in the region is unknown. A systematic review and case studies from Brazil, Argentina, Uruguay, and Chile were conducted to address the following objectives: (1) quantify adoption of existing DA technologies, (2) identify limitations for DA adoption; and (3) summarise existing metrics to benchmark DA benefits. Level of DA adoption was led by Brazil and Argentina followed …

Cropland Reference Ecological Unit: A Land Classification Unit For Comparative Soil Studies, Saurav Das, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

There is a growing consensus on a need for measuring the dynamic soil properties of croplands and even comparisons with a reference state or native land. These measurements and paired comparisons will create the capacity to determine soil health management effects and targets. However, the complex soil heterogeneity and climate variations make soil health potential variable and confound the effects of land-use and management practices and comparisons between soils from different sites. Identifying a discrete landmass unit where all soils have similar health potential will be critical in conducting meaningful comparative studies and measuring the impact of conservation practices. This …

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Herbaceous Production Lost To Tree Encroachment In United States Rangelands, Scott L. Morford, Brady W. Allred, Dirac L. Twidwell Jr, Matthew O. Jones, Jeremy D. Maestas, Caleb P. Roberts, David E. Naugle

Department of Agronomy and Horticulture: Faculty Publications

1. Rangelands of the United States provide ecosystem services that benefit society and rural economies. Native tree encroachment is often overlooked as a primary threat to rangelands due to the slow pace of tree cover expansion and the positive public perception of trees. Still, tree encroachment fragments these landscapes and reduces herbaceous production, thereby threatening habitat quality for grassland wildlife and the economic sustainability of animal agriculture.

2. Recent innovations in satellite remote sensing permit the tracking of tree encroachment and the corresponding impact on herbaceous production. We analysed tree cover change and herbaceous production across the western United States …

From Free-Energy Profiles To Activation Free Energies, Johannes C. Dietschreit, Dennis J. Diestler, Andreas Hulm, Christian Ochsenfeld, Rafael Gómez-Bombarelli

Department of Agronomy and Horticulture: Faculty Publications

Given a chemical reaction going from reactant (R) to the product (P) on a potential energy surface (PES) and a collective variable (CV) discriminating between R and P, we define the free-energy profile (FEP) as the logarithm of the marginal Boltzmann distribution of the CV. This FEP is not a true free energy. Nevertheless, it is common to treat the FEP as the “free-energy” analog of the minimum potential energy path and to take the activation free energy, ΔF‡ _RP, as the difference between the maximum at the transition state and the minimum at R. We show that this …

A Yield Comparison Between Small-Plot And On-Farm Foliar Fungicide Trials In Soybean And Maize, Anabelle Laurent, Emily Heaton, Peter Kyveryga, David Makowski, Laila A. Puntel, Alison E. Robertson, Laura J. Thompson, Fernando Miguez

Department of Agronomy and Horticulture: Faculty Publications

Agronomic research provides management recommendations based on small-plot trials (SPTs) and on-farm trials (OFTs) with very different characteristics. SPTs are traditionally conducted at agricultural experiment stations by research institutes or universities, while OFTs are conducted under commercial-scale conditions and managed by farmers using their own equipment. Several researchers claimed that discrepancies could occur between these two types of trials, which can make the extrapolation of results from SPTs to the farm level difficult. In our study, we conducted an extensive comparison of small-plot and on-farm trials to analyze the effect of foliar fungicide application on maize and soybean yields. We …

Snp Discovery In Proso Millet (Panicum Miliaceum L.) Using Lowpass Genome Sequencing, Rituraj Khound, Guangchao Sun, Ravi V. Mural, James C. Schnable, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Domesticated ~10,000 years ago in northern China, Proso millet (Panicum miliaceum L.) is a climate-resilient and human health-promoting cereal crop. The genome size of this self-pollinated allotetraploid is 923 Mb. Proso millet seeds are an important part of the human diet in many countries. In the USA, its use is restricted to the birdseed and pet food market. Proso millet is witnessing gradual demand in the global human health and wellness food market owing to its health-promoting properties such as low glycemic index and gluten-free. The breeding efforts for developing improved proso millet cultivars are hindered by the …

The Genetic Basis For Panicle Trait Variation In Switchgrass (Panicum Virgatum), Li Zhang, Alice Macqueen, Xiaoyu Weng, Kathrine D. Behrman, Jason Bonnette, John L. Reilley, Francis M. Rouquette, Philip A. Fay, Yanqi Wu, Felix B. Fritschi, Robert B. Mitchell, David B. Lowry, Arvid R. Boe, Thomas E. Juenger

Department of Agronomy and Horticulture: Faculty Publications

Key message: We investigate the genetic basis of panicle architecture in switchgrass in two mapping populations across a latitudinal gradient, and find many stable, repeatable genetic effects and limited genetic interactions with the environment. Abstract: Grass species exhibit large diversity in panicle architecture influenced by genes, the environment, and their interaction. The genetic study of panicle architecture in perennial grasses is limited. In this study, we evaluate the genetic basis of panicle architecture including panicle length, primary branching number, and secondary branching number in an outcrossed switchgrass QTL population grown across ten field sites in the central USA through multi-environment …

Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan Mcpherson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Arbuscular mycorrhizal fungi (AMF) remain a vital obligate symbiont of nearly all plants. It is well established that the symbiosis between AMF and host plant improves plant nutrient acquisition, alleviates abiotic and biotic environmental stressors, defends against plant pathogens, and contributes to overall plant fitness and productivity through modification of the soil habitat. Modifications include increased soil aggregation and stability, carbon sequestration through provision of fungal wall precursors to soil organic matter (SOM) formation, and enhanced nutrient cycling in the mycorrhizosphere. The goal of this dissertation was to assess how AMF respond to nitrogen (N) fertilization regimes in maize cropping …

Quantification And Machine Learning Based N2o–N And Co2–C Emissions Predictions From A Decomposing Rye Cover Crop, Deepak R. Joshi, David E. Clay, Sharon A. Clay, Janet Moriles-Miller, Aaron L. M. Daigh, Graig Reicks, Shaina Westhoff

Department of Agronomy and Horticulture: Faculty Publications

Cover crops improve soil health and reduce the risk of soil erosion. However, their impact on the carbon dioxide equivalence (CO_2e) is unknown. Therefore, the objective of this 2-yr study was to quantify the effect of cover crop-induced differences in soil moisture, temperature, organic C, and microorganisms on CO_2e, and to develop machine learning algorithms that predict daily N₂O–N and CO₂–C emissions. The prediction models tested were multiple linear regression, partial least square regression, support vector machine, random forest (RF), and artificial neural network. Models’ performance was accessed using R², …

Registration Of Lcs ‘Valiant’ Hard Red Winter Wheat, P. Stephen Baenziger, S. D. Masterson, J. D. Boehm Jr., Vikas Belamkar, M. D. Barnett, Devin J. Rose, Lan Xu, Stephen N. Wegulo, T. Regassa, Amanda Easterly, Cody F Creech, Dipak K. Santra, G. R. Kruger, G. W. Hergert, R. N. Klein, J. Kolmer, J. Kolmer, M.-S. Chen, Gary L. Hein, R. L. Bowden, M. J. Guttieri, G. Bai, El-Basyoni Salah, J. Poland

Department of Agronomy and Horticulture: Faculty Publications

Historically cultivars developed jointly by the University of Nebraska-Lincoln and USDA-ARS wheat (Triticum aestivum L.) improvement project tend to be late and better adapted to the northern Great Plains. LCS ‘Valiant’ (Reg. no. CV-1196, PI 693223; tested as NE10478-1) was released based on the merits of its earliness, agronomic performance, relevant disease resistances, and end-use quality characteristics and its adaptation to the central Great Plains. As such, the line was licensed to Limagrain Cereal Seeds for their ability to market outside of Nebraska. LCS Valiant hard red winter wheat was released in March 2020 by the developing institutions and …

Optimization Of Nozzle, Application Height, And Speed For Uass Pesticide Applications, Trenton Houston

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Unmanned aerial spray systems (UASS) applications have the potential to be efficient pesticide application platforms under conditions that are not accessible or fit for typical pesticide application equipment. Although this type of application is still under development in the U.S., UASS pesticide applications are common in Asia, as they have replaced backpack sprayers. There is limited literature on the optimization of UASS applications and many parameters need to be investigated to identify the best combination of application variables such as flight height, flight speed, and nozzle selection. The objectives were to identify the deposition patterns of a four rotor UASS …

Association Analyses Of Host Genetics, Root-Colonizing Microbes, And Plant Phenotypes Under Different Nitrogen Conditions In Maize, Michael A. Meier, Gen Xu, Martha G. Lopez-Guerrero, Guangyong Li, Christine Smith, Brandi Sigmon, Joshua R. Herr, James R. Alfano, Yufeng Ge, James C. Schnable, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

The root-associated microbiome (rhizobiome) affects plant health, stress tolerance, and nutrient use efficiency. However, it remains unclear to what extent the composition of the rhizobiome is governed by intraspecific variation in host plant genetics in the field and the degree to which host plant selection can reshape the composition of the rhizobiome. Here, we quantify the rhizosphere microbial communities associated with a replicated diversity panel of 230 maize (Zea mays L.) genotypes grown in agronomically relevant conditions under high N (+N) and low N (-N) treatments. We analyze the maize rhizobiome in terms of 150 abundant and consistently reproducible …

Replacing Fallow With Field Pea In Wheat Production Systems Across Western Nebraska, Samuel T. Koeshall, Amanda Easterly, Rodrigo Werle, Strahinja Stepanovic, Cody F. Creech

Department of Agronomy and Horticulture: Faculty Publications

Integration of field pea (Pisum sativum L.) (FP) into dryland cropping systems has increased due to ecological and economic benefits, paired with a growing market for pea-derived products. Challenges exist in the High Plains that limit the integration of crop rotations to replace fallow periods with FP in wheat (Triticum aestivum L.)- based systems. This experiment compares chemical summer fallow to FP in a fallow– wheat rotation at two locations in western Nebraska. Soil water content, soil fertility, N mineralization, FP yield, and subsequent hard red winter wheat (HWW) yields were recorded. Subsequent HWW yields were not different …

Non-Destructive Classification And Quality Evaluation Of Proso Millet Cultivars Using Nir Hyperspectral Imaging With Machine Learning, Laruen E. Doyle, Julia R. Loeb, Nader Ekramirad, Dipak K. Santra, Akinbode A. Adedeji

Department of Agronomy and Horticulture: Faculty Publications

Millet is a small-seeded cereal crop with big potential and remarkable characteristics such as high drought resistance, short growing time, low water footprint, and the ability to grow in acidic soil. There is a need to develop nondestructive methods for differentiation and evaluation of the quality attributes of different of proso millet cultivars grown in the U.S. Current methods of cultivar classification are either subjective or destructive, time consuming, not allowing for the whole population to be tested, and requiring trained operators and special equipment. In this study, the feasibility of using near-infrared (NIR) hyperspectral imaging (900-1700 nm) to predict …

Characterization And Selection Of Hop Cultivars Adapted To Nebraska, Kristina Alas

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Hop (Humulus lupulus L.) is an ingredient in the beer brewing industry that provides beer its flavor and aroma. High demand from the brewing industry has encouraged production outside of the traditional Pacific Northwest, the primary production region. Producers in the Midwest are attempting to grow cultivars adapted to the Pacific Northwest, but environmental differences have caused low yields and changes in secondary metabolite content. To aid producers, a regional breeding program was initiated to develop cultivars adapted to the Midwest. Success of any breeding program relies on the selection of genetically superior parents to generate progeny with the …

Dicamba Tank Mixtures And Formulations And Their Effects On Sensitive Crops During Cleanout Procedures, Vinicius Velho

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The introduction of dicamba-tolerant (DT) soybeans (Glycine max L. Merr) and cotton (Gossypium hirsutum L) in 2017 provided an additional tool for herbicide resistant weeds management. In the subsequent years, off-target movement of dicamba allegedly caused damage to sensitive crops and vegetation.

Possible causes of off-target movement include tank contamination, physical drift, and volatility. Additional products, such as herbicides to control grass, are often added to tank with dicamba, which is used to control broadleaf weeds, to increase the spectrum of control and application efficiency. Dicamba products registered for DT crops require the use of drift reducing …

Increased Signal-To-Noise Ratios Within Experimental Field Trials By Regressing Spatially Distributed Soil Properties As Principal Components, Jeffrey C. Berry, Mingsheng Qi, Balasaheb V. Sonawane, Amy Sheflin, Asaph Cousins, Jessica Prenni, Daniel P. Schachtman, Peng Liu, Rebecca S. Bart

Department of Agronomy and Horticulture: Faculty Publications

Environmental variability poses a major challenge to any field study. Researchers attempt to mitigate this challenge through replication. Thus, the ability to detect experimental signals is deter-mined by the degree of replication and the amount of environmental variation, noise, within the experimental system. A major source of noise in field studies comes from the natural heterogeneity of soil properties which create microtreatments throughout the field. In addition, the variation within different soil properties is often nonrandomly distributed across a field. We explore this challenge through a sorghum field trial dataset with accompanying plant, microbiome, and soil property data. Diverse sorghum …

Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang

Department of Agronomy and Horticulture: Faculty Publications

Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo’s responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and …

Neuropilin-2 Regulates Androgen-Receptor Transcriptional Activity In Advanced Prostate Cancer, Samikshan Dutta, Navatha Shree Polavaram, Ridwan Islam, Sreyashi Bhattacharya, Sanika Bodas, Thomas Mayr, Sohini Roy, Sophie Alvarez, Marieta I. Toma, Anza Darehshouri, Angelika Borkowetz, Stefanie Conrad, Susanne Fuessel, Manfred Wirth, Gustavo B. Baretton, Paramita Ghosh, Kenneth J. Pienta, David L. Klinkebiel, Surinder K. Batra, Michael H. Muders, Kaustubh Datta

Department of Agronomy and Horticulture: Faculty Publications

Aberrant transcriptional activity of androgen receptor (AR) is one of the dominant mechanisms for developing of castration-resistant prostate cancer (CRPC). Analyzing AR-transcriptional complex related to CRPC is therefore important towards understanding the mechanism of therapy-resistance. While studying its mechanism, we observed that a transmembrane protein called neuropilin-2 (NRP2) plays a contributory role in forming a novel AR-transcriptional complex containing nuclear pore proteins. Using immunogold electron microscopy, high-resolution confocal microscopy, chromatin immunoprecipitation, proteomics, and other biochemical techniques, we delineated the molecular mechanism of how a specific splice variant of NRP2 becomes sumoylated upon ligand stimulation and translocates to the inner nuclear …

Identification Of Putative Snp Markers Associated With Resistance To Egyptian Loose Smut Race(S) In Spring Barley, Kamal A.M. Abo-Elyousr, Amira M.I. Mourad, P. Stephen Baenziger, Abdelaal H.A. Shehata, Peter E. Eckstein, Aaron D. Beattie, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Loose smut (LS) disease is a serious problem that affects barley yield. Breeding of resistant cultivars and identifying new genes controlling LS has received very little attention. Therefore, it is important to understand the genetic basis of LS control in order to genetically improve LS resistance. To address this challenge, a set of 57 highly diverse barley genotypes were inoculated with Egyptian loose smut race(s) and the infected seeds/plants were evaluated in two growing seasons. Loose smut resistance (%) was scored on each genotype. High genetic variation was found among all tested genotypes indicating considerable differences in LS resistance that …

Sorghum Association Panel Whole-Genome Sequencing Establishes Cornerstone Resource For Dissecting Genomic Diversity, J. Lucas Boatwright, Sirjan Sapkota, Hongyu Jin, James C. Schnable, Zachary Brenton, Richard Boyles, Stephen Kresovich

Department of Agronomy and Horticulture: Faculty Publications

Association mapping panels represent foundational resources for understanding the genetic basis of phenotypic diversity and serve to advance plant breeding by exploring genetic variation across diverse accessions. We report the whole-genome sequencing (WGS) of 400 sorghum (Sorghum bicolor (L.) Moench) accessions from the Sorghum Association Panel (SAP) at an average coverage of 38× (25–72×), enabling the development of a high-density genomic marker set of 43 983 694 variants including single-nucleotide polymorphisms (approximately 38 million), insertions/deletions (indels) (approximately 5 million), and copy number variants (CNVs) (approximately 170 000). We observe slightly more deletions among indels and a much higher prevalence …

Water Quality, Carrier Volume And Droplet Size Effects On Herbicide Efficacy And Drift Potential, Barbara Houston

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Herbicide performance is directly related to the amount of active ingredient that has been deposited on the plant. Hence, spray solution characteristics and application parameters are crucial in determining the efficacy of an herbicide application. To maximize the effectiveness of chemical control, methods to deliver full chemical dose must be utilized: allowing the active ingredient to be readily absorbed once added to the carrier and mitigating off-target movement and low herbicide doses. Water is the most frequently used carrier in herbicide applications. Chemical parameters, such as water hardness and pH, can have a critical role in herbicide performance. It is …

Recombination Hotspots In Soybean [Glycine Max (L.) Merr.], Samantha J. Mcconaughy

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Recombination allows for the exchange of genetic material between two parents which plant breeders exploit to make new and improved varieties. This recombination is not distributed evenly across the chromosome. In crops, it mostly occurs in the euchromatic regions of the genome and even then, recombination is focused into recombination hotspots flanked by recombination cold spots. Understanding the distribution of these hotspots along with the sequence motifs associated with them may lead to methods that enable breeders to better exploit recombination in breeding.

In chapter 1 background information on recombination, recombination hotspots detection methods, landscape of recombination (describe recombination patterns …

Regional Plant Community Differences In The Nebraska Sandhills, Travis Millikan

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The Nebraska Sandhills is very valuable to the state of Nebraska, representing one of the most in-tact and largest grassland ecosystems in temperate regions in the world. Rangeland managers must understand plant community dynamics across the Sandhills to better inform management decisions. The first objective of this study was to evaluate plant community variability on upland Sands ecological sites across different precipitation zones in the Nebraska Sandhills. The second objective of our study was to utilize the Rangeland Analysis Platform (RAP) to examine spatial and temporal variability in biomass production and cover on pastures of ranches analyzed in the first …

Site-Specific Pest Management In Nebraska Corn And Soybean Production Systems, Zachary D. Rystrom

Doctor of Plant Health Program: Dissertations and Student Research

Site-specific management (SSM) is widely used by farm producers to fertilize their fields. However, whole field management is currently practiced in integrated pest management (IPM). Site-specific management and agricultural technology can improve IPM especially when precision application of inputs can reduce selection pressure on pest populations, benefit the environment, or save costs of inputs. There is potential for site-specific pest management (SSPM) where pests, or environments vary spatially, and recommended management practices can be applied with precision. Three case studies are evaluated for SSPM to be applied in Nebraska corn and soybean production systems including corn rootworm, preemergence herbicides, and …

Chromosome-Level Thlaspi Arvense Genome Provides New Tools For Translational Research And For A Newly Domesticated Cash Cover Crop Of The Cooler Climates, Adam Nunn, Isaac Rodríguez-Arévalo, Zenith Tandukar, Katherine Anna Frels, Adrián Contreras-Garrido, Pablo Carbonell-Bejerano, Panpan Zhang, Daniela Ramos Cruz, Katharina Jandrasits, Christa Lanz, Anthony Brusa, Marie Mirouze, Kevin Dorn, David W. Galbraith, Brice A. Jarvis, John C. Sedbrook, Donald L. Wyse, Christian Otto, David Langenberger, Peter F. Stadler, Detlef Weigel, M. David Marks, James A. Anderson, Claude Becker, Ratan Chopra

Department of Agronomy and Horticulture: Faculty Publications

Thlaspi arvense (field pennycress) is being domesticated as a winter annual oilseed crop capable of improving ecosystems and intensifying agricultural productivity without increasing land use. It is a selfing diploid with a short life cycle and is amenable to genetic manipulations, making it an accessible field-based model species for genetics and epigenetics. The availability of a high-quality reference genome is vital for understanding pennycress physiology and for clarifying its evolutionary history within the Brassicaceae. Here, we present a chromosome-level genome assembly of var. MN106-Ref with improved gene annotation and use it to investigate gene structure differences between two accessions (MN108 …