Open Access. Powered by Scholars. Published by Universities.®
- Institution
-
- Old Dominion University (20)
- Chapman University (18)
- Himmelfarb Health Sciences Library, The George Washington University (9)
- Selected Works (8)
- City University of New York (CUNY) (7)
-
- East Tennessee State University (4)
- SelectedWorks (4)
- The Texas Medical Center Library (4)
- University of Connecticut (4)
- University of Kentucky (3)
- Rowan University (2)
- Virginia Commonwealth University (2)
- Aga Khan University (1)
- Butler University (1)
- California Polytechnic State University, San Luis Obispo (1)
- Duquesne University (1)
- Florida International University (1)
- Marshall University (1)
- Missouri State University (1)
- Murray State University (1)
- Purdue University (1)
- Roseman University of Health Sciences (1)
- Technological University Dublin (1)
- The University of Maine (1)
- Trinity College (1)
- University of Georgia School of Law (1)
- University of Louisville (1)
- University of Southern Maine (1)
- University of Tennessee, Knoxville (1)
- Western Michigan University (1)
- Keyword
-
- Animals (8)
- Biology (5)
- Cellular biology (4)
- Anatomy and physiology (3)
- Biological sciences (3)
-
- Calcium (3)
- Cells (3)
- Cilia (3)
- Collagen (3)
- Gene expression (3)
- Innate immunity (3)
- Metabolism (3)
- Mitochondria (3)
- Neurons (3)
- ROS (3)
- Anatomy (2)
- Antigens (2)
- Atoh1 (2)
- Cerebellum (2)
- Ciliopathy (2)
- Development (2)
- Endothelium (2)
- Genes (2)
- Glutamatergic neurons (2)
- Granule cells (2)
- Heart (2)
- Human (2)
- Humans (2)
- Hydrogen peroxide (2)
- Hypoxia (2)
- Publication Year
- Publication
-
- Pharmacy Faculty Articles and Research (12)
- Anatomy and Regenerative Biology Faculty Publications (9)
- Electronic Theses and Dissertations (6)
- Theses and Dissertations in Biomedical Sciences (6)
- School of Medical Diagnostics & Translational Sciences Faculty Publications (5)
-
- Arthur M. Mercurio (4)
- Dissertations & Theses (Open Access) (4)
- George McNamara (4)
- Biological Sciences Theses & Dissertations (3)
- Health Sciences and Kinesiology Faculty Articles (3)
- Honors Scholar Theses (3)
- Open Educational Resources (3)
- Bioelectrics Publications (2)
- Biological Sciences Faculty Publications (2)
- Mathematics, Physics, and Computer Science Faculty Articles and Research (2)
- Publications and Research (2)
- Annual Research Symposium (1)
- Biology, Chemistry, and Environmental Sciences Faculty Articles and Research (1)
- Center for Research on Environmental Disease Faculty Publications (1)
- Chancellor’s Honors Program Projects (1)
- Chemistry & Biochemistry Theses & Dissertations (1)
- Computer Science Faculty Publications (1)
- Department of Pathology and Laboratory Medicine (1)
- Dissertations and Theses (1)
- FIU Electronic Theses and Dissertations (1)
- Gyongyi Szabo (1)
- Honors Theses (1)
- Jennifer Kowalski (1)
- Katherine A. Fitzgerald (1)
- Katherine Campbell, PhD (1)
Articles 91 - 104 of 104
Full-Text Articles in Cell Biology
Insulinlike Growth Factor 1- And 2-Augmented Collagen Gel Repair Of Facial Osseous Defects, James S. Toung, Roy C. Ogle, Raymond F. Morgan, William H. Lindsey
Insulinlike Growth Factor 1- And 2-Augmented Collagen Gel Repair Of Facial Osseous Defects, James S. Toung, Roy C. Ogle, Raymond F. Morgan, William H. Lindsey
School of Medical Diagnostics & Translational Sciences Faculty Publications
BACKGROUND: Defects of the facial bone structure are common problems for the facial plastic surgeon. Native type 1 collagen gels (T1CGs) have been shown to mediate repair of facial critical-size defects in rat models.
OBJECTIVE: To evaluate the efficacy of T1CG augmented with insulinlike growth factor (IGF) 1, IGF-2, and a combination of IGF-1 and IGF-2 on the repair of facial critical-size defects in a rodent model.
METHODS: Twenty-four retired male breeder Sprague-Dawley rats were divided into 4 groups of 6 animals. Facial critical-size defects were created by removing the nasalis bones with a bone-cutting drill. Defects were treated with …
Efficacy Of AllowashTm Solution In Solubilization Of Bone Marrow, Alyce Marie Linthurst
Efficacy Of AllowashTm Solution In Solubilization Of Bone Marrow, Alyce Marie Linthurst
Biological Sciences Theses & Dissertations
LifeNet currently cleans allograft bone utilizing AllowashTM Technology, which encompasses the use of a closed continuous circulation chamber to remove bone marrow by flushing it with AllowashTM Solution for a prescribed time and temperature after which, it is rinsed with various solutions to remove residual detergent that could be toxic to the recipient. A portion of this process employs AllowashTM Solution to improve the solubilization and removal of protein and cellular elements in the red bone marrow. This study examines the effectiveness of AllowashTM Solution in comparison to its component detergents Nonidet P40, Nonoxynol 9 and …
Pressure-Induced Intracellular Signaling In Isolated Arteries, Victor A. Miriel
Pressure-Induced Intracellular Signaling In Isolated Arteries, Victor A. Miriel
Theses and Dissertations in Biomedical Sciences
The ability of cells to respond to mechanical stimuli has been studied through a variety of techniques in numerous cell types. The cells of the vascular wall have adapted to specific mechanical stresses through the activation of intracellular signaling pathways which result in cell-specific responses such as hypertrophy, hyperplasia, proliferation, and migration. Vascular smooth muscle of the arteries have been shown to be sensitive to mechanical stimuli such as stretch, and pressure.
This study attempts to add to the current knowledge of mechanotransduction by utilizing the isolated artery preparation. This preparation allows for the study of vascular smooth muscle signal …
The Role Of Glycoconjugates In Mediating Human Fertilization And Induction Of Fetomaternal Tolerance, Manish S. Patankar
The Role Of Glycoconjugates In Mediating Human Fertilization And Induction Of Fetomaternal Tolerance, Manish S. Patankar
Theses and Dissertations in Biomedical Sciences
Using the hemizona assay (HZA), a in vitro sperm-egg binding assay, we show that specific glycoconjugates known to inhibit immune cell interactions mediated by the selectins, potently block human sperm-egg binding. The selectin ligand sialyl Lewisx inhibits sperm binding in the HZA by 60% at a concentration of 1 mg/ml. Our data indicates that glycodelin-A, a endometrial glycoprotein known to block sperm-egg binding in the HZA at low concentrations expresses unusual fucosylated lacdiNAc type glycans. The fucosylated lacdiNAc type sugars have been previously shown to be 15-20 fold more potent ligands of E-selectin. Glycodelin-S a seminal plasma glycoform of …
A Kinetic Investigation Of The Mechanism Of Muscle Contraction With A Series Of Nucleotides, Wei Jiang
A Kinetic Investigation Of The Mechanism Of Muscle Contraction With A Series Of Nucleotides, Wei Jiang
Theses and Dissertations in Biomedical Sciences
Muscle contraction is thought to be accomplished by sliding of myosin filaments along actin filaments. Although actomyosin naturally uses ATP as an energy source, it can also use other nucleoside triphosphates (NTP) as substrates. In this work, the dependence of rate and equilibrium constants of the various steps in the muscle contraction mechanism upon nucleotide structure was investigated to unravel the dependence of the mechanical properties of the muscle upon changes in different biochemical steps of the mechanism.
The experiments measuring the dissociation of actomyosin by NTP (or mant-NTP) shows that both the rate constant of actomyosin-S1 dissociation and second …
Cellular Mechanisms Underlying Myogenic Reactivity In Isolated Arterioles, Hui Zou
Cellular Mechanisms Underlying Myogenic Reactivity In Isolated Arterioles, Hui Zou
Theses and Dissertations in Biomedical Sciences
The myogenic reactivity provides one of the principal mechanisms for blood flow autoregulation. The aims of the performed studies described in this dissertation were to test the role of [Ca2+]i and MLC phosphorylation in arteriolar myogenic reactivity and further examine the source(s) of activator Ca2+ required to initiate and maintain myogenic vasoconstriction. In addition, the possible underlying mechanism of contractile protein expression was also addressed.
These studies used male Sprague Dawley rats of 200 ~ 350 grams body weight. Experiments were carried out using rat cremaster first order arterioles and mesenteric vessels. Gel electrophoresis and immunoblotting …
Microcirculation: Electrophysiological Basis For The Response Of Endothelial Cells To Inflammatory Mediators-Bradykinin, Kai Miao
Electronic Theses and Dissertations
Using conventional microelectrodes, I studied the electrical basis for determining the resting V$\sb{\rm m}$ in intact EC's from hamsters. The resting V$\sb{\rm m}$ were found to be $-$40 mV for aortic EC's and $-$43 mV for vena caval EC's. The contributions of ions to the resting V$\sb{\rm m}$ of aortic EC's were compared in terms of the transference number (t$\sb{\rm ion}$). To develop a technique for in situ monitoring changes in V$\sb{\rm m}$ of postcapillary venular EC's in the hamster mesentery, a voltage-sensitive fluorescent probe, bisoxonol, was used to load the cells and the fluorescence signals were analyzed under an …
Changes In Intracellular Chloride During Osmotic Stress And L-Alanine Uptake In Mouse Hepatocytes, Kening Wang
Changes In Intracellular Chloride During Osmotic Stress And L-Alanine Uptake In Mouse Hepatocytes, Kening Wang
Electronic Theses and Dissertations
A stable intracellular ionic environment is necessary for hepatocytes to function normally. Thus, during hypotonic shock or L-alanine uptake, hepatocytes swell and then exhibit a regulatory volume decrease (RVD), which comprises an increase in K$\sp+$ conductance (G$\sb{\rm K}$), an increased K$\sp+$ efflux, and a hyperpolarization of transmembrane potential (V$\sb{\rm m}$). Since hepatocyte intracellular Cl$\sp-$ has been demonstrated to distribute passively with V$\sb{\rm m}$, this study is designed to test the hypothesis that the hypotonic shock- or L-alanine uptake-induced hyperpolarization of V$\sb{\rm m}$ might provide an electromotive force for the efflux of hepatocyte intracellular Cl$\sp-$, which in turn would contribute osmotically …
Osteoinductivity Of Demineralized Bone: A Quantitative In Vitro Assessment, Yihong Zheng
Osteoinductivity Of Demineralized Bone: A Quantitative In Vitro Assessment, Yihong Zheng
Biological Sciences Theses & Dissertations
Acid demineralized bone (DMB) implanted in extraosseous sites induces bone formation. In vivo studies have shown that DMB stimulates the differentiation of mesenchymal cells into osteogenic cells. In this study an in vitro system was developed to demonstrate the osteoinductivity of DMB quantitatively. This in vitro working system was used to assess the osteoinductivity of variously processed DMB products as a means to evaluate their processing protocols. A human dermal fibroblastic (HDF) cell line was initiated from tissue explants as a working model, along with a human periosteal (HPO) cell line as a control. Ground DMB processed by LifeNet Transplant …
Electrophysiology, Cell Calcium, And Mechanisms Of Hepatocyte Volume Regulation, Walid E. Khalbuss
Electrophysiology, Cell Calcium, And Mechanisms Of Hepatocyte Volume Regulation, Walid E. Khalbuss
Electronic Theses and Dissertations
The electrophysiologic technique (Reuss, L., Proc. Natl. Acad. Sci. USA 82:6014, 1985) was modified to measure changes in steady-state hepatocyte volume during osmotic stress. Hepatocytes in mouse liver slices were loaded with tetramethylammonium ion (TMA$\sp{+}$) during transient exposure of cells to nystatin. Intracellular TMA$\sp{+}$ activity (a$\sp{\rm i}\sb{\rm TMA}$) was measured with TMA$\sp{+}$-sensitive, double-barreled microelectrodes. Loading hepatocytes with TMA$\sp{+}$ did not change their membrane potential (V$\sb{\rm m}$), and under steady-state conditions a$\sp{\rm i}\sb{\rm TMA}$ remained constant over 4 min in single impalements. Hyperosmotic solutions (50, 100, & 150 mM sucrose added to media) and hyposmotic solutions (sucrose in media reduced by …
Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little
Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little
School of Medical Diagnostics & Translational Sciences Faculty Publications
Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment of cells adherent to a planar substratum. This process of antibody-mediated substratum detachment resulted in a clustering of CBP and cell-associated extracellular matrix at the cell surface, and the rearrangement of filamentous actin. Other functional studies showed that cells grown within a three-dimensional gel of type I collagen cannot be immunostained at the cell surface with CBP …
Collagen Binding Proteins Derived From The Embryonic Fibroblast Cell Surface Recognize Arginine-Glycine-Aspartic Acid, Roy C. Ogle, Charles D. Little
Collagen Binding Proteins Derived From The Embryonic Fibroblast Cell Surface Recognize Arginine-Glycine-Aspartic Acid, Roy C. Ogle, Charles D. Little
School of Medical Diagnostics & Translational Sciences Faculty Publications
Several cell surface proteins (Mr = 120,000, 90,000, 63,000 and 47,000) apparently integral to embryonic fibroblast plasma membranes were extracted with detergent and isolated by collagen affinity chromatography. Certain of these proteins (Mr = 120,000, 90,000, and 47,000) were specifically eluted from collagen affinity columns by synthetic peptides containing the amino acid sequence arginyl-glycyl-aspartic acid (RGD). These data show that a number of collagen binding proteins exist on the embryonic fibroblast cell surface. Some of the proteins may be collagen receptors binding to RGD sequences in the collagen molecule while at least one of the proteins (Mr = 63,000) recognizes …
Laminin Receptors For Neurite Formation, H. K. Kleinman, Roy C. Ogle, F. B. Cannon, C. D. Little, T. M. Sweeney, L. Luckenbill-Edds
Laminin Receptors For Neurite Formation, H. K. Kleinman, Roy C. Ogle, F. B. Cannon, C. D. Little, T. M. Sweeney, L. Luckenbill-Edds
School of Medical Diagnostics & Translational Sciences Faculty Publications
Laminin, a basement membrane glycoprotein promotes both cell attachment and neurite outgrowth. Separate domains on laminin elicit these responses, suggesting that distinct receptors occur on the surface of cells. NG108-15 neuroblastoma-glioma cells rapidly extend long processes in the presence of laminin. We report here that 125I-labeled laminin specifically binds to these cells and to three membrane proteins of 67, 110, and 180 kDa. These proteins were isolated by affinity chromatography on laminin-Sepharose. The 67-kDa protein reacted with antibody to the previously characterized receptor for cell attachment to laminin. Antibodies to the 110-kDa and 180-kDa bands demonstrated that the 110-kDa protein …
The Effects Of Luteinizing Hormone And Adenosine 3',5'-Cyclic Monophosphate On Phospholipid Metabolism By Luteal Mitochondria, Jim John Sadighian
The Effects Of Luteinizing Hormone And Adenosine 3',5'-Cyclic Monophosphate On Phospholipid Metabolism By Luteal Mitochondria, Jim John Sadighian
Chemistry & Biochemistry Theses & Dissertations
Luteinizing hormone (LH) increases intracellular concentrations of adenosine 3', 5 '-cyclic monophosphate and the phosphoinositides, phosphatidylinositol (PI), PI 4'-phosphate (PIP) and PI 4' .5 1 - bispbosphate (PIP2). It is believed that cAMP and the phosphoinositides act concertedly to regulate mitochondrial conversion of cholesterol to pregnenolone. This study examined the effects of LH and N6 ,O2 -dibutyryl cAMP (dbcAMP) on phospholipids metabolism by luteal mitochondria and the influence of dbcAMP and the phosphoinosi tides on mitochondrial steroid production. Mitochondria were isolated from unincubated and incubated luteal tissue by differential centrifugation. Phospholipids were extracted from the mitochondria …