Articles 1 - 1 of 1
Full-Text Articles in Molecular Biology
Characterization And Application Of A Novel Cholesterol-Reducing Anaerobe, Eubacterium Coprostanoligenes Atcc 51222 , Ling Li
Retrospective Theses and Dissertations
A novel cholesterol-reducing bacterium, Eubacterium coprostanoligenes ATCC 51222, was characterized and tested for its hypocholesterolemic effects in animal models. The bacterium did not require cholesterol for growth and synthesized cholesterol reductase constitutively. Lecithin was required for growth but excess lecithin in media did not improve bacterial growth and coprostanol production. Addition of pyruvate, lactose, and most reducing agents in media increased bacterial growth and coprostanol production. The bacterium was sensitive to most antibiotics but had strong resistance to tetracycline. A resting cell assay was established to evaluate the cholesterol reductase activity. E. coprostanoligenes reduced cholesterol through an indirect pathway. Intermediates ...