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Articles 1 - 7 of 7

Full-Text Articles in Molecular Biology

Study Of The Structural Requirements Of E Coli Valine Transfer Rna In Aminoacylation And Protein Synthesis , Mingsong Liu Jan 1995

Study Of The Structural Requirements Of E Coli Valine Transfer Rna In Aminoacylation And Protein Synthesis , Mingsong Liu

Retrospective Theses and Dissertations

The structural requirements for the function of the 3[superscript]' CCA end of E. coli valine transfer RNA in aminoacylation and protein synthesis have been investigated, by studying the activities of 3[superscript]' end tRNA[superscript] Val variants in these processes. These studies show that 3[superscript]' end tRNA[superscript] Val variants can be obtained which are functional in both aminoacylation and later steps of protein synthesis. Replacement of the 3[superscript]' terminal adenosine with either cytosine or uracil yields tRNAs that retain almost full aminoacylation activity (40-50% that of wild type tRNA[superscript] Val). The tRNA[superscript] Val variant ...


Identification And Characterization Of G4-Dna Binding Proteins From Tetrahymena Thermophila , Ted Patrick Schierer Jan 1995

Identification And Characterization Of G4-Dna Binding Proteins From Tetrahymena Thermophila , Ted Patrick Schierer

Retrospective Theses and Dissertations

Alternative DNA conformations have been known to exist since before the elucidation of the double helix. The study of alternative DNA conformations formed by telomeric sequences has led to detailed studies of biologically relevant DNA capable of forming unusual structures. In order to understand the biological role of alternative DNA conformations formed by telomeric DNA, proteins which bind these structures have been identified and characterized;The initial portion of my research involved the identification of a protein from Tetrahymena thermophila which binds preferentially to parallel-stranded G-quartet structures. Competition and binding assays demonstrated that the protein, TGP (Tetrahymena G4 binding protein ...


Characterization And Application Of A Novel Cholesterol-Reducing Anaerobe, Eubacterium Coprostanoligenes Atcc 51222 , Ling Li Jan 1995

Characterization And Application Of A Novel Cholesterol-Reducing Anaerobe, Eubacterium Coprostanoligenes Atcc 51222 , Ling Li

Retrospective Theses and Dissertations

A novel cholesterol-reducing bacterium, Eubacterium coprostanoligenes ATCC 51222, was characterized and tested for its hypocholesterolemic effects in animal models. The bacterium did not require cholesterol for growth and synthesized cholesterol reductase constitutively. Lecithin was required for growth but excess lecithin in media did not improve bacterial growth and coprostanol production. Addition of pyruvate, lactose, and most reducing agents in media increased bacterial growth and coprostanol production. The bacterium was sensitive to most antibiotics but had strong resistance to tetracycline. A resting cell assay was established to evaluate the cholesterol reductase activity. E. coprostanoligenes reduced cholesterol through an indirect pathway. Intermediates ...


Calsensin, A Novel Calcium-Binding Protein Expressed In A Subset Of Peripheral Leech Neurons Fasciculating In A Single Axon Tract , Kristen Kay Briggs Jan 1995

Calsensin, A Novel Calcium-Binding Protein Expressed In A Subset Of Peripheral Leech Neurons Fasciculating In A Single Axon Tract , Kristen Kay Briggs

Retrospective Theses and Dissertations

The lan 3-6 monoclonal antibody recognizes a subpopulation of peripheral neurons whose afferents segregate into a single fascicle within the developing leech central nervous system. The lan 3-6 monoclonal antibody was used to isolate a clone encoding a novel protein, calsensin, which contains two EF-hand calcium-binding domains. In situ hybridizations with calsensin riboprobes, Western blots of lan 3-6 monoclonal antibody immunoprecipitations, and immunohistochemistry with polyclonal antibodies generated against a partially-purified calsensin fusion protein confirmed that the sequence encoding the lan 3-6 antigen has been identified. Partially-purified calsensin fusion protein binds [superscript]45Ca[superscript]2+ in vitro, suggesting that the calsensin ...


Sequence Variation Characteristics Of D-Loop, Rrna, Trna, And Polypeptide Coding Region Of Bovine Mitochondrial Dna , Jianming Wu Jan 1995

Sequence Variation Characteristics Of D-Loop, Rrna, Trna, And Polypeptide Coding Region Of Bovine Mitochondrial Dna , Jianming Wu

Retrospective Theses and Dissertations

By cloning and sequencing the mtDNA of cows from different maternal lineages, it was found that multiple heteroplasmic mtDNA states within the D-loop and rRNA genes of mtDNA existed extensively in Holstein cattle. Meanwhile, the nature and transmission of mtDNA were investigated. The mtDNA population in a specific animal was found to be a mixture of different genotypes. Because heteroplasmy was observed frequently and seemingly is persistent, selected amplification of specific mtDNA molecules may not be a part of mtDNA inheritance. At same time, four hypervariable sites were located at nt 169, 216, and 1594 between nt 352 to 364 ...


Biochemical And Molecular Biological Characterization Of Plant Acetyl-Coa Carboxylases , James J. Caffrey Jan 1995

Biochemical And Molecular Biological Characterization Of Plant Acetyl-Coa Carboxylases , James J. Caffrey

Retrospective Theses and Dissertations

Acetyl-CoA carboxylase is a biotin-containing enzyme which catalyzes the ATP-dependent carboxylation of acetyl-CoA to form malonyl-CoA. Plants are unique among organisms in that malonyl-CoA is utilized in both de novo fatty acid biosynthesis, and as an intermediate in a number of other plant primary and secondary metabolic pathways. Some of these metabolic pathways operate in different subcellular and cellular compartments. Therefore, plants have a mechanism to generate separate pools of malonyl-CoA. Understanding this mechanism has been the impetus for the research reported in this dissertation;Immunological and structural analyses of a 60 kDa biotinylated polypeptide (60kDBP) from carrot indicate that ...


Elm1p, A Protein Kinase That Regulates Cell Morphology In Saccharomyces Cerevisiae , Carla Marie Koehler Jan 1995

Elm1p, A Protein Kinase That Regulates Cell Morphology In Saccharomyces Cerevisiae , Carla Marie Koehler

Retrospective Theses and Dissertations

Saccharomyces cerevisiae is a pleimorphic ascomycete, capable of growing in the yeast form or the pseudohyphal form. The pseudohyphal form is characterized by elongated cells that possess a unipolar budding pattern and grow in chains away from the center of the colony. This change in morphology is induced by nitrogen starvation in the presence of carbon. A collection of 14 elm (elongated morphology) mutants previously have been isolated that grow as pseudohyphae constitutively. Of these mutants, the product of the ELM1 gene was identified and coded for a novel protein kinase. From genetic analyses, ELM1 seemingly functioned downstream of STE7 ...