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Molecular Biology Commons

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Theses/Dissertations

And developmental biology

Nutrition

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Full-Text Articles in Molecular Biology

Characterization And Application Of A Novel Cholesterol-Reducing Anaerobe, Eubacterium Coprostanoligenes Atcc 51222 , Ling Li Jan 1995

Characterization And Application Of A Novel Cholesterol-Reducing Anaerobe, Eubacterium Coprostanoligenes Atcc 51222 , Ling Li

Retrospective Theses and Dissertations

A novel cholesterol-reducing bacterium, Eubacterium coprostanoligenes ATCC 51222, was characterized and tested for its hypocholesterolemic effects in animal models. The bacterium did not require cholesterol for growth and synthesized cholesterol reductase constitutively. Lecithin was required for growth but excess lecithin in media did not improve bacterial growth and coprostanol production. Addition of pyruvate, lactose, and most reducing agents in media increased bacterial growth and coprostanol production. The bacterium was sensitive to most antibiotics but had strong resistance to tetracycline. A resting cell assay was established to evaluate the cholesterol reductase activity. E. coprostanoligenes reduced cholesterol through an indirect pathway. Intermediates ...


Studies On The Role Of Expression Of Phosphoenolpyruvate Carboxykinase Gene In The Development Of Bovine Lactation Ketosis , Sowmya Moorthi Jan 1992

Studies On The Role Of Expression Of Phosphoenolpyruvate Carboxykinase Gene In The Development Of Bovine Lactation Ketosis , Sowmya Moorthi

Retrospective Theses and Dissertations

Changes in expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene, which codes for a key regulatory enzyme of gluconeogenesis, were studied in bovine liver tissue. Induction of ketosis was attempted in cows by a protocol of restricting feed intake (FR) and supplementing with 1,3-butanediol (BD), a ketogenic precursor, beginning at 15 d postpartum (FRBD protocol). Twenty Holstein cows were distributed in equal numbers among four treatments: normal control, obese control, normal cows given FRBD, and obese cows given FRBD. Liver biopsies were obtained at nine different times during early lactation: 10 d prepartum, 5 and 14 d postpartum (just before ...