Life Sciences Commons^™

Mccoy, Thomas N. (Sc 2635), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 2635. Bound typescript of paper by Thomas N. McCoy, Catlettsburg, Kentucky entitled “Sadie F. Price, 1849-1903,” Kentucky Botanist,” including typescripts of correspondence with Price about botany, and a collection of new clippings concerning Price.

Group Cohesion And Homework Adherence In Multi-Family Group Therapy For Schizophrenia, Frank P. Deane, Joanne Mercer, Anahita Talyarkhan, Gordon Lambert, Judy Pickard

Frank Deane

This study examined the relationship between levels of group cohesion, defined as whole group relationships, and between-session therapeutic homework adherence in a multi-family group therapy (MFGT) for people with schizophrenia. Participants from 18 consenting families attending MFGT groups completed weekly homework adherence ratings, group cohesion and spontaneous between-session activity measures. Levels of group cohesion at each session were compared with measures of scheduled and spontaneous homework adherence reported at the next session. It was hypothesised that higher levels of group cohesion would be related to homework adherence and other spontaneous between-session therapeutic activity completed by group members. Results show higher …

Study Protocol: A Randomized Controlled Trial Of A Computer-Based Depression And Substance Abuse Intervention For People Attending Residential Substance Abuse Treatment, Peter J. Kelly, Frances Kay-Lambkin, Amanda Baker, Frank P. Deane, Adam C. Brooks, Alexandra Mitchell, Sarah Marshall, Meredith Whittington, Genevieve A. Dingle

Frank Deane

"Background: A large proportion of people attending residential alcohol and other substance abuse treatment have a co-occurring mental illness. Empirical evidence suggests that it is important to treat both the substance abuse problem and co-occurring mental illness concurrently and in an integrated fashion. However, the majority of residential alcohol and other substance abuse services do not address mental illness in a systematic way. It is likely that computer delivered interventions could improve the ability of substance abuse services to address co-occurring mental illness. This protocol describes a study in which we will assess the effectiveness of adding a computer delivered …

A Base Composition Analysis Of Natural Patterns For The Preprocessing Of Metagenome Sequences, Oliver Bonham-Carter, Hesham Ali, Dhundy Raj Bastola

Information Systems and Quantitative Analysis Faculty Publications

Background: On the pretext that sequence reads and contigs often exhibit the same kinds of base usage that is also observed in the sequences from which they are derived, we offer a base composition analysis tool. Our tool uses these natural patterns to determine relatedness across sequence data. We introduce spectrum sets (sets of motifs) which are permutations of bacterial restriction sites and the base composition analysis framework to measure their proportional content in sequence data. We suggest that this framework will increase the efficiency during the pre-processing stages of metagenome sequencing and assembly projects. Results: Our method is able …

Neuroprotective Effects Of Long-Term Endurance Training On The Cortical Autonomic Network In The Aging Brain, Torri A. Luchyshyn

Electronic Thesis and Dissertation Repository

This study tested whether long-term endurance training in older adults (ET; n = 15, 55 ± 4 years, relative VO₂max = 50 ± 8 ml/kg/min) would alter cardiovagal control and preserve the cortical autonomic network compared to age-matched controls (CON; n = 15, 56 ± 4 years, relative VO₂max = 37 ± 9 ml/kg/min). The hypothesis predicts 1) altered deactivation patterns of the ventral medial prefrontal cortex (vMPFC) in response to isometric hand grip (IHG) and 2) greater indices of cardiovagal control; a) increased baroreflex sensitivity at rest, b) greater heart rate change (ΔHR) and c) …

Efficacy And Costs Of Controlling Eastern Redcedar, John Ortmann, James L. Stubbendieck, Robert A. Masters, George H. Pfeiffer, Thomas B. Bragg

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

(Juniperus virginiana L.) is reducing grassland productivity across much of the Great Plains. Control methods include broadcast prescribed fire, herbicides, cutting, and individual tree ignition. All methods have disadvantages when used alone. Fire can be ineffective against larger trees. Intensive methods can be too expensive for low-productivity grasslands. The objectives of this research were to determine the effects of broadcast prescribed fie alone as measured at 3 weeks after fire; to compare the effects of picloram herbicide application with or without fire, sawing with or without fire, and individual tree ignition with fire; and to compare all treatment costs. …

A Proposed Mechanism For Nitrogen Acquisition By Grass Seedlings Through Oxidation Of Symbiotic Bacteria, James F. White Jr., Holly Crawford, Mónica S. Torres, Robert Mattera, Ivelisse Irizarry, Marshall Bergen

All Faculty Scholarship for the College of the Sciences

In this paper we propose and provide evidence for a mechanism, oxidative nitrogen scavenging (ONS), whereby seedlings of some grass species may extract nitrogen from symbiotic diazotrophic bacteria through oxidation by plant-secreted reactive oxygen species (ROS). Experiments on this proposed mechanism employ tall fescue (Festuca arundinaceae) seedlings to elucidate features of the oxidative mechanism. We employed 15N₂ gas assimilation experiments to demonstrate nitrogen fixation, direct microscopic visualization of bacteria on seedling surfaces to visualize the bacterial oxidation process, reactive oxygen probes to test for the presence of H₂O₂ and cultural experiments to assess conditions …

Sp605-P Lead Poisoning Can Hurt Your Child Bookmark, Martha Keel

Environment (Indoor & Outdoor)

No abstract provided.

Analyzing Self-Similar And Fractal Properties Of The C. Elegans Neural Network, Tyler M. Reese, Antoni Brzoska, Daniel J. Kelleher

Open Access Author Fund Awardees' Articles

The brain is one of the most studied and highly complex systems in the biological world. While much research has concentrated on studying the brain directly, our focus is the structure of the brain itself: at its core an interconnected network of nodes (neurons). A better understanding of the structural connectivity of the brain should elucidate some of its functional properties. In this paper we analyze the connectome of the nematode Caenorhabditis elegans. Consisting of only 302 neurons, it is one of the better-understood neural networks. Using a Laplacian Matrix of the 279-neuron “giant component” of the network, we …

Sp605-F Keys To Healthy Eating, Martha Keel, Janie Burney, Janice Mccoy

Health, and Fitness

No abstract provided.

Sp605-A What Do You Know About Lead Poisoning? Common Myths, Misunderstandings And The Facts, Martha Keel, Janice Mccoy

Environment (Indoor & Outdoor)

No abstract provided.

Sp605-B Preventing Lead Poisioning, Martha Keel, Janice Mccoy

Environment (Indoor & Outdoor)

No abstract provided.

Rr13-01 Research Report: Wheat Variety Perfomance Tests In Tennessee 2012, Fred L. Allen, Richard D. Johnson, Robert C. Williams Jr., Chris Main

Field & Commercial Crops

No abstract provided.

Brainstorm: Head Injuries And The Nfl, Part 8: Cutting­Edge Nerves, John J. Medina Ph.D.

Brainstorm

Armed with information from the last installment about nerve cells’ basic biology, we can now talk about how they get injured in a more informed fashion. That’s the subject of this post, and also the next two.

Cartilage On The Move: Cartilage Lineage Tracing During Tadpole Metamorphosis, Ryan R. Kerney, Alison L. Brittain, Brian K. Hall, Daniel R. Buchholz

Biology Faculty Publications

The reorganization of cranial cartilages during tadpole metamorphosis is a set of complex processes. The fates of larval cartilage-forming cells (chondrocytes) and sources of adult chondrocytes are largely unknown. Individual larval cranial cartilages may either degenerate or remodel, while many adult cartilages appear to form de novo during metamorphosis. Determining the extent to which adult chondrocytes/cartilages are derived from larval chondrocytes during metamorphosis requires new techniques in chondrocyte lineage tracing. We have developed two transgenic systems to label cartilage cells throughout the body with fluorescent proteins. One system strongly labels early tadpole cartilages only. The other system inducibly labels forming …

Regulatory Elements Of Xenopus Col2a1 Drive Cartilaginous Gene Expression In Transgenic Frogs, Ryan R. Kerney, Brian K. Hall, James Hanken

Ryan Kerney

This study characterizes regulatory elements of collagen 2α1 (col2a1) in Xenopus that enable transgene expression in cartilage-forming chondrocytes. The reporters described in this study drive strong cartilage-specific gene expression, which will be a valuable tool for further investigations of Xenopus skeletal development. While endogenous col2a1 mRNA is expressed in many embryonic tissues, its expression becomes restricted to tadpole and adult chondrocytes. This chondrocyte-specific expression is recapitulated by col2a1 reporter constructs, which were tested through I-SceI meganuclease-mediated transgenesis. These constructs contain a portion of the Xenopus tropicalis col2a1 intron, which aligns to a cartilage-specific intronic enhancer that has been well characterized …

Visual And Electronic Evaluations Of Detached Strawberry Leaves Inoculated With Colletotrichum Species, M. A. Miller-Butler, K. J. Curry, B. R. Kreiser, B. J. Smith

Faculty Publications

Inoculation of detached strawberry leaves with Colletotrichum species may provide an accurate, rapid, non-destructive method of identifying anthracnose resistant germplasm. Two assessments of anthracnose disease severity were compared on detached strawberry leaves inoculated with Colletotrichum fragariae and C. gloeosporioides: a quantitative assessment made via computer-based image analysis and a visual assessment made by two independent raters. The image analysis provided a precise measurement of percent lesion area of infected leaves. There was a strong positive correlation between percent lesion area and the visual disease scores of the raters.

Meeting Minutes, October 3rd, 2012, National Smokejumper Association Board Of Directors

National Smokejumper Association Meeting Minutes

Agenda: Orientation to facility/introductions/approval of agenda.; Additions to the agenda will be added to the second day's business.; By-law update.; Database report.; Membership report.; Report on the NSA Al Dunton Smokejumper Leadership award.; Update on FS Fire Program.; Website Committee report.; Website minority report.; Historical preservation activity & face book updating .; Trail report.; Missoula Jumper Reunion planning for 2013.; Magazine and merchandising report.; Report on feasibility of paid Executive Director for NSA.; Treasurer report and report from.; Financial report re: investment policy.; October 4th Second day of meeting.; Clean up any issues not resolved during the prior day's meeting.; …

Genetic Diversity Of Neotropical Myotis (Chiroptera: Vespertilionidae) With An Emphasis On South American Species, Roxanne J. Larsen, Michelle C. Knapp, Hugh H. Genoways, Faisal Ali Anwarali Khan, Peter A. Larsen, Don E. Wilson, Robert J. Baker

University of Nebraska State Museum: Mammalogy Papers

Background: Cryptic morphological variation in the Chiropteran genus Myotis limits the understanding of species boundaries and species richness within the genus. Several authors have suggested that it is likely there are unrecognized species-level lineages of Myotis in the Neotropics. This study provides an assessment of the diversity in New World Myotis by analyzing cytochrome-b gene variation from an expansive sample ranging throughout North, Central, and South America. We provide baseline genetic data for researchers investigating phylogeographic and phylogenetic patterns of Myotis in these regions, with an emphasis on South America.

Methodology and Principal Findings: Cytochrome-b sequences were generated and …

Regional Development And Local Government: Three Generations Of Federal Intervention, Andrew H. Kelly, Brian Dollery, Bligh Grant

Bligh Grant

Contemporary Australian local government faces several daunting problems, not least escalating financial un-sustainability and local infrastructure depletion. The main response of the various state and territory governments has taken the form of a series structural reform programs, with a strong emphasis on forced amalgamation. However, widespread dissatisfaction with the consequences of these compulsory consolidation programs has led to a search for alternative policy solutions based largely on shared services and various types of regional co-operation between local councils. This paper seeks to place proposed ‘regional’ solutions to contemporary problems in historical perspective by providing a comparative account of three distinct …

Simple, Compact Source For Low-Temperature Air Plasmas, D. Sheehan, J. Lawson, M. Sosa, Richard Long

Richard A. Long

A simple, compact source of low-temperature, spatially and temporally uniform air plasma using a Telsa induction coil driver is described. The low-power ionization discharge plasma is localized (2 cm X 0.5 cm X 0.1 cm) and essentially free of arc channels. A Teflon coated rolling cylindrical electrode and dielectric coated ground plate are essential to the source’s operation and allow flat test samples to be readily exposed to the plasma. The plasma is a copious source of ozone and nitrogen oxides. Its effects on various microbes are discussed.

Individual Cell Growth Rates Of Marine Bacteria, Measured By Bromodeoxyuridine Incorporation, Koji Hamasaki, Richard A. Long, Farooq Azam

Richard A. Long

We tested the application of 5-bromo-2’-deoxyuridine (BrdU), a thymidine analogue that becomes incorporated into DNA during growth, to measure growth rates of individual marine bacteria cells. Immunocytochemical detection of BrdU incorporation into bacterial DNA has the potential for single-cell-based growth measurement. Optimized procedure for immunocytochemistry was applicable to 14 marine heterotrophic bacterial isolates belonging to g-proteobacteria, α-proteobacteria, Cytophaga-Flavobacterium-Bacteroides (CFB) group and Gram-positive bacteria. The relationship between cell-specific fluorescence intensity and specific growth rate was linearly correlated among CFB group isolates, which indicated a potential of the method for quantitative measurement. Analysis of the detection limit indicated …

Coupling Between Bacterioplankton Species Composition, Population Dynamics, And Organic Matter Degradation, Jarone Pinhassi, Farooq Azam, Johanna Hemphälä, Richard A. Long, Josefina Martinez, Ulla Li Zweifel, Åke Hagström

Richard A. Long

To study the effect of substrate addition on short term bacterial population dynamics and species composition in seawater mesocosms were maintained with water collected off Scripps Pier (La Jolla, California USA) Protein enrichment (BSA) triggered a dynamic response from the microbial food web, whereas enrichment with starch had no effect. In the protein enriched mesocosm the number of both nucleoid-containing cells and metabolically active cells increased by 3.0 X 105 cells ml-1 from Day 1 to Day 4 In the same time period the density of a set of 31 phylogenetically different bacteria (α- and g-Proteobactena as well as Flexibacter-Cytophaga-Bacteroides) …

Abundant Protein-Containing Particles In The Sea, Richard A. Long, Farooq Azam

Richard A. Long

The interaction of bacteria with particulate organic matter has implications for organic matter cycling and bacterial ecology in the ocean. Until recently, the focus has been on 'classical' particles visible by unaided eye (marine snow) or light microscopy. Recent discoveries of several new types of abundant particles, from sub-micrometer to sub-marine snow, are changing our ideas of the physical and chemical nature of the particle field with which pelagic bacteria interact. Previous workers have discovered polysaccharide-containing (Alcian Blue stainable) transparent exopolymer particles (TEP) ranging from 3 to 100s of micrometers. Looking for additional components of the sub-marine snow particle field, …

U.S. Drought Monitor, October 2, 2012, Anthony Artusa

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for October 2, 2012 (10/2/2012) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Hay, Cattle, Winter wheat.

The Institutional Repository: Open Access’S Green Alternative, Douglas T. Macbeth

Douglas Thomas Macbeth

No abstract provided.

Iqgap Family Members In Yeast, Dictyostelium, And Mammalian Cells, Katie Shannon

Biological Sciences Faculty Research & Creative Works

IQGAPs are a family of scaffolding proteins with multiple domains, named for the IQ motifs and GTPase activating protein (GAP) related domains. Despite their GAP homology, IQGAP proteins act as effectors for GTP-bound GTPases of the Ras superfamily and do not stimulate GTP hydrolysis. IQGAPs are found in eukaryotic cells from yeast to human, and localize to actin-containing structures such as lamellipodia, membrane ruffles, cell-cell adhesions, phagocytic cups, and the actomyosin ring formed during cytokinesis. Mammalian IQGAPs also act as scaffolds for signaling pathways. IQGAPs perform their myriad functions through association with a large number of proteins including filamentous actin …

Prelude To A Master Plan: Ware, Massachusetts, Belen Alfaro, Bruno Carneiro, Margaret Engesser, Kathryn E. Fox, Evadne R. Friedman, Timothy Inacio, Anita Lockesmith, Christina Mills, Stephanie Molden, Meagen Mulherin, Russell Pandres, Vinicius Pereira, Brian Reid, Pedro Soto, Jennifer Stromsten

Landscape Architecture & Regional Planning Studio and Student Research and Creative Activity

Prelude to a Master Plan offers ideas, recommendations, and a toolkit to help the town chart its own path towards that future. While the teams and individual students worked to ‘drill down’ into specific topic areas, the Studio defined three basic areas in order to think about how the various assets, challenges and ideas undermine or reinforce one another. The report is loosely organized in those terms: addressing the outlying rural areas and issues specific to these places, considering one of the key growth areas that has extended from town and the conflicts that arise from the many uses occurring …

Derivation Of Screening Benchmarks For Dietary Methylmercury Exposure For The Common Loon (Gavia Immer): Rationale For Use In Ecological Risk Assessment, David C. Depew, Niladri Basu, Neil M. Burgess, Linda M. Campbell

University Faculty Publications and Creative Works

The current understanding of methylmercury (MeHg) toxicity to avian species has improved considerably in recent years and indicates that exposure to environmentally relevant concentrations of MeHg through the diet can adversely affect various aspects of avian health, reproduction, and survival. Because fish-eating birds are at particular risk for elevated MeHg exposure, the authors surveyed the available primary and secondary literature to summarize the effects of dietary MeHg on the common loon (Gavia immer) and to derive ecologically relevant toxic thresholds for dietary exposure to MeHg in fish prey. After considering the available data, the authors propose three screening benchmarks of …

Bicatalytic Allylation–Cross-Metathesis Reactions As Γ-Carbonyl Cation Equivalents, Jake R. Henkie, Sugadar Dhaliwal, James R. Green

Chemistry and Biochemistry Publications

The products corresponding to the reactions of arenes and γ-carbonyl cations may be obtained by a one-pot, bicatalytic process involving InCl₃-catalyzed arene allylation and cross metathesis with electron-deficient alkenes. The process is successful with electronically neutral and electron-rich arenes, and modestly Lewis basic donor groups are tolerated with an increase in InCl₃ loading from 10 mol% to 15 mol%, and in one case, 20 mol%.