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2012

California Polytechnic State University, San Luis Obispo

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Articles 1 - 30 of 137

Full-Text Articles in Life Sciences

How Cultured Dairy Products Can Have A Long Shelf Life And A Clean Label, Phillip S. Tong Dec 2012

How Cultured Dairy Products Can Have A Long Shelf Life And A Clean Label, Phillip S. Tong

Dairy Science

No abstract provided.


Traversing Swanton Road, 10th Ed., James A. West Dec 2012

Traversing Swanton Road, 10th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …


Dietary Fat Composition Influences Tissue Lipid Profile And Gene Expression In Fischer-344 Rats, Albert L. Zhou, Korry J. Hintze, Rafael Jiménez-Flores, Robert E. Ward Dec 2012

Dietary Fat Composition Influences Tissue Lipid Profile And Gene Expression In Fischer-344 Rats, Albert L. Zhou, Korry J. Hintze, Rafael Jiménez-Flores, Robert E. Ward

Dairy Science

The AIN-76A diet causes fatty liver in rodents when fed for long periods of time. The aimof this study was to utilize fatty acid analysis and transcriptomics to investigate the effects of different fat sources in the AIN-76A diet on tissue lipid profiles and gene expression in male, weanling Fischer-344 rats. Animals were fed isocaloric diets that differed only in the fat source: (1) corn oil (CO) (2) anhydrous milk fat (AMF), and (3) AMF supplemented with 10 % phospholipids from the milk fat globule membrane (AMFMFGM). There were no differences in food intake, body weight, growth rate, or body …


Graduate Internship Report- Selma High School, Jalisca Jean Thomason Dec 2012

Graduate Internship Report- Selma High School, Jalisca Jean Thomason

Agricultural Education: Graduate Internship Reports

This internship report addresses the quality criteria of an agricultural education program and includes the supporting documents to meet the requirements of the California Department of Education specific to Selma High School. This document and the supporting evidence is intended to meet the requirements to obtain maximum funding for the Ag Incentive Grant.


Landscape Design, Cost Estimate, And Management Plan, Garrett Talbot Williams Dec 2012

Landscape Design, Cost Estimate, And Management Plan, Garrett Talbot Williams

Horticulture and Crop Science

The purpose of this project is to evolve a bleak landscape into something that the home owner can appreciate and love. This project will demonstrate the knowledge of this process to provide as a guide for others. This project gives an overview of all aspects of a landscape project including: drawings of an irrigation layout, planting plan, drainage plan, and a construction plan. This project takes place in Riverbank, California, which is located in the Central Valley. The property’s main centerpiece is a pool and adjacent waterfall. However, the landscape currently surrounding the pool is very bare and lacks aesthetic …


Effects Of Grazing Vs. Resting On Soil Chemistry And Plant Community Dynamics For The Central Coast Of California In 2012, Jessica Combs Dec 2012

Effects Of Grazing Vs. Resting On Soil Chemistry And Plant Community Dynamics For The Central Coast Of California In 2012, Jessica Combs

Animal Science

No abstract provided.


Mortality Assessment Of Redwood And Mixed Conifer Forest Types In Santa Cruz County Following Wildfire, Steve R. Auten Dec 2012

Mortality Assessment Of Redwood And Mixed Conifer Forest Types In Santa Cruz County Following Wildfire, Steve R. Auten

Master's Theses

On August 12, 2009, the Lockheed Fire ignited the west slope of the Santa Cruz Mountains burning approximately 7,819 acres. Foresters and other land managers were left with challenging decisions on how to evaluate tree mortality. Big Creek Lumber Company, California Polytechnic State University, San Luis Obispo (Cal Poly)’s Swanton Pacific Ranch (SPR), and other resource professionals familiar with this region teamed up to develop a method for evaluating damage and thereby mortality for redwood, California nutmeg, live oak, tanoak, California bay, Pacific madrone, big leaf maple, Douglas-fir, Monterey pine, and knobcone pine. Quantitative damage criteria were used to design …


Post-Fire Mortality And Response In A Redwood/ Douglas-Fir Forest, Santa Cruz Mountains, California, Garren M. Andrews Dec 2012

Post-Fire Mortality And Response In A Redwood/ Douglas-Fir Forest, Santa Cruz Mountains, California, Garren M. Andrews

Master's Theses

We investigated how fire severity impacts the survival and response (sprouting/seeding) of multiple species in the Santa Cruz Mountains of coastal California, including coast redwood (Sequoia sempervirens), Douglas-fir (Pseudotsuga menziesii), tanoak (Lithocarpus densiflorus), and Pacific madrone(Arbutus menziesii). During August 2009 the Lockheed Fire burned nearly 3,160ha of mixed-conifer stands with variable severity. Data from 37 Continuous Forest Inventory (CFI) plots were collected immediately before and for 2 successive years following the 2009 Lockheed Fire.

This research entails three objectives. First, we quantified post-fire mortality of trees that vary in species, size, and …


Low Cost Neurochairs, Frankie Pike Dec 2012

Low Cost Neurochairs, Frankie Pike

Master's Theses

Electroencephalography (EEG) was formerly confined to clinical and research settings with the necessary hardware costing thousands of dollars. In the last five years a number of companies have produced simple electroencephalograms, priced below $300 and available direct to consumers. These have stirred the imaginations of enthusiasts and brought the prospects of "thought-controlled" devices ever closer to reality. While these new devices were largely targeted at video games and toys, active research on enabling people suffering from debilitating diseases to control wheelchairs was being pursued. A number of neurochairs have come to fruition offering a truly hands-free mobility solution, but whether …


House Finches, Carpodacus Mexicanus: Hormones, Stress, And Song Control Regions, Katherine Olivia Ganster Dec 2012

House Finches, Carpodacus Mexicanus: Hormones, Stress, And Song Control Regions, Katherine Olivia Ganster

Master's Theses

Song production in songbirds is controlled by parts of the brain known as the song control regions (SCRs). During spring, gonads increase in size, males sing to attract mates, and SCRs become larger. This neuroplasticity is controlled by the change in day length and increased plasma testosterone (T) levels. Plasma T can be reduced by stress through the production of corticosterone (CORT), through the production of beta-endorphin, or through direct effects on the testes via the nervous system. We determined the T, estradiol, and CORT hormonal profiles of wild House Finches by capturing and sampling blood from the finches every …


Effects Of Silvicultural Management On Coast Redwood Forest Composition, Density And Structure In Santa Cruz And San Mateo Counties, Michael J. Papa Dec 2012

Effects Of Silvicultural Management On Coast Redwood Forest Composition, Density And Structure In Santa Cruz And San Mateo Counties, Michael J. Papa

Master's Theses

The multiple-objective exploratory study investigates effects of various silvicultural management regimes commonly applied to coast redwood (Sequoia sempervirens [D. Don] Endl.) forests in Santa Cruz and San Mateo Counties, California, USA. A temporary forest inventory was installed in 24 harvest origin stands and 4 natural origin stands throughout the study area (sample area = 1189 acres). Data from the systematic sample of 233 one-quarter acre nested cluster plots (sample intensity = 4.9%) rendered overall forest descriptions in terms of species composition, density, and structure. The common forestry measures of trees per acre (TPA), basal area per acre (BA), and …


An Analysis Of Human Disturbance To Rocky Intertidal Communities Of San Luis Obispo County, Grant Tyler Waltz Dec 2012

An Analysis Of Human Disturbance To Rocky Intertidal Communities Of San Luis Obispo County, Grant Tyler Waltz

Master's Theses

ABSTRACT

An Analysis Of Human Disturbance To Rocky Intertidal Communities Of San Luis Obispo County

Grant Tyler Waltz

The number of coastal areas open to public access in California and San Luis Obispo County is increasing due to the acquisition by California State Parks of land previously owned by private entities. For example, California State Parks acquired property from the Hearst Corporation in 2005, which included 18 miles of coastline. California State Parks is responsible for providing public access in these newly acquired areas and also for maintaining the health of the natural systems found on these properties. Part of …


Food Fortification Improves The Intake Of All Fortified Nutrients, But Fails To Meet The Estimated Dietary Requirements For Vitamins A And B6, In Lactating South African Women, Peggy C. Papathakis, Kerry E. Pearson Oct 2012

Food Fortification Improves The Intake Of All Fortified Nutrients, But Fails To Meet The Estimated Dietary Requirements For Vitamins A And B6, In Lactating South African Women, Peggy C. Papathakis, Kerry E. Pearson

Food Science and Nutrition

Objective To investigate the impact of fortification by comparing food records and selected biochemical indicators of nutritional status pre- and post-fortification.

Design Mean intake from 24 h recalls (n 142) was compared with the Estimated Average Requirement (EAR) to determine the proportion with inadequate intake. In a subsample (n 34), diet and serum retinol, folate, ferritin and Zn were compared pre- and post-fortification for fortified nutrients vitamin A, thiamin, riboflavin, niacin, folic acid, Fe and Zn.

Setting South Africa.

Subjects Breast-feeding women (ninety-four HIV-infected, forty eight HIV-uninfected) measured at ∼6, 14, 24 weeks, and 9 and 12 months …


Production Of Highly Concentrated, Heat-Stable Hepatitis B Surface Antigen In Maize, Celine A. Hayden, Erin M. Egelkrout, Alessa M. Moscoso, Cristina Enrique, Todd K. Keener, Rafael Jiménez-Flores, Jeffrey C. Wong, John A. Howard Oct 2012

Production Of Highly Concentrated, Heat-Stable Hepatitis B Surface Antigen In Maize, Celine A. Hayden, Erin M. Egelkrout, Alessa M. Moscoso, Cristina Enrique, Todd K. Keener, Rafael Jiménez-Flores, Jeffrey C. Wong, John A. Howard

Dairy Science

Plant-based oral vaccines are a promising emergent technology that could help alleviate disease burden worldwide by providing a low-cost, heat-stable, oral alternative to parenterally administered commercial vaccines. Here, we describe high-level accumulation of the hepatitis B surface antigen (HBsAg) at a mean concentration of 0.51%TSP in maize T1 seeds using an improved version of the globulin1 promoter. This concentration is more than fourfold higher than any previously reported lines. HBsAg expressed in maize seeds was extremely heat stable, tolerating temperatures up to 55 °C for 1 month without degradation. Optimal heat stability was achieved after oil extraction of ground maize …


Regulated Deficit Irrigation Effect On Yield And Wine Color Of Cabernet Sauvignon In Central California, Michael J. Costello, W. Keith Patterson Oct 2012

Regulated Deficit Irrigation Effect On Yield And Wine Color Of Cabernet Sauvignon In Central California, Michael J. Costello, W. Keith Patterson

Wine and Viticulture

Regulated deficit irrigation (RDI) is a management strategy that on grape can improve shoot/fruit ratio, water efficiency, and wine quality but has the potential to reduce yield. As part of a study on the influence of RDI on leafhopper density, we evaluated the effects on grape yield, berry size, berry soluble solids, and wine color. The studies were conducted at commercial vineyards in the San Joaquin Valley and in the Paso Robles region, CA, with Cabernet Sauvignon as the cultivar. Water deficits were imposed at either 50% (moderate deficit) or 25% (severe deficit) of standard irrigation (the control) for a …


Rapid Invasion Of Indo-Pacific Lionfishes (Pterois Volitans And Pterois Miles) In The Florida Keys, Usa: Evidence From Multiple Pre- And Post-Invasion Data Sets, Benjamin I. Ruttenberg, Pamela J. Schofield, J. Lad Akins, Alejandro Acosta, Michael W. Feeley, Jeremiah Blondeau, Steven G. Smith, Jerald S. Ault Oct 2012

Rapid Invasion Of Indo-Pacific Lionfishes (Pterois Volitans And Pterois Miles) In The Florida Keys, Usa: Evidence From Multiple Pre- And Post-Invasion Data Sets, Benjamin I. Ruttenberg, Pamela J. Schofield, J. Lad Akins, Alejandro Acosta, Michael W. Feeley, Jeremiah Blondeau, Steven G. Smith, Jerald S. Ault

Biological Sciences

Over the past decade, Indo-Pacific lionfishes, Pterois volitans (Linnaeus, 1758) and Pterois miles (Bennett, 1828), venomous members of the scorpionfish family (Scorpaenidae), have invaded and spread throughout much of the tropical and subtropical northwestern Atlantic Ocean and Caribbean Sea. These species are generalist predators of fishes and invertebrates with the potential to disrupt the ecology of the invaded range. Lionfishes have been present in low numbers along the east coast of Florida since the 1980s, but were not reported in the Florida Keys until 2009. We document the appearance and rapid spread of lionfishes in the Florida Keys using multiple …


Dynamics Of An Acute Coral Disease Outbreak Associated With The Macroalgae Dictyota Spp. In Dry Tortugas National Park, Florida, Usa, Marilyn E. Brandt, Benjamin I. Ruttenberg, Rob Waara, Jeff Miller, Brian Witcher, Andrew J. Estep, Matt Patterson Oct 2012

Dynamics Of An Acute Coral Disease Outbreak Associated With The Macroalgae Dictyota Spp. In Dry Tortugas National Park, Florida, Usa, Marilyn E. Brandt, Benjamin I. Ruttenberg, Rob Waara, Jeff Miller, Brian Witcher, Andrew J. Estep, Matt Patterson

Biological Sciences

Reports of coral disease outbreaks have increased in recent decades, but often few details are known about these outbreaks, such as environmental triggers, associated biological variables, or even the precise temporal span of the outbreak. Here we document an acute outbreak of a rapid tissue loss disease on the highest live coral cover (15%–30%) reefs within Dry Tortugas National Park, Florida, USA. This disease exhibited similar signs to white plague disease with the notable exception that a white film often was observed on the recently denuded skeleton. The temporal span of the disease was short (<2 mo). Partial mortality was recorded but there was no detectable impact to overall coral cover. A significant increase and then decrease in the cover of macroalgae, primarily of the genus Dictyota, occurred simultaneously …


An Experimental Evaluation Of The Use Of C3 Δ13C Plant Tissue As A Proxy For The Paleoatmospheric Δ13Co2 Signature Of Air, B. H. Lomax, C. A. Knight, J. A. Lake Sep 2012

An Experimental Evaluation Of The Use Of C3 Δ13C Plant Tissue As A Proxy For The Paleoatmospheric Δ13Co2 Signature Of Air, B. H. Lomax, C. A. Knight, J. A. Lake

Biological Sciences

Previous work suggests that the relationship between the carbon isotope composition of air (d13Ca) and plant leaf tissue (d13Cp) can be used to track changes in the carbon isotope composition of paleo-atmospheric CO2. Here we test this assertion in a series of experiments using the model plant Arabidopsis thaliana grown under a range of atmospheric CO2 concentrations relevant to geologic time (380, 760, 1000, 1500, 2000 and 3000 ppm). Nested within these CO2 experiments water availability was controlled (giving two sets of experimental plants; low and high water treatment at each CO2 concentration) to manipulate stomatal opening, a key process …


Cultures And Stabilizers In Cultured Dairy Foods, Phillip S. Tong Sep 2012

Cultures And Stabilizers In Cultured Dairy Foods, Phillip S. Tong

Dairy Science

No abstract provided.


Environmental Stress Proteomics Of Two Blue Mussel (Genus Mytilus) Congeners, Lars Tomanek Sep 2012

Environmental Stress Proteomics Of Two Blue Mussel (Genus Mytilus) Congeners, Lars Tomanek

Biological Sciences

Abstract of paper presented at the 28th Congress of the New European Society of Comparative Physiology and Biochemistry Proceedings: Bilbao, Spain.


Gene Transcripts Encoding Hypoxia-Inducible Factor (Hif) Exhibit Tissue- And Muscle Fiber Type-Dependent Responses To Hypoxia And Hypercapnic Hypoxia In The Atlantic Blue Crab, Callinectes Sapidus, Kristin M. Hardy, Chandler R. Follett, Louis E. Burnett, Sean Lema Sep 2012

Gene Transcripts Encoding Hypoxia-Inducible Factor (Hif) Exhibit Tissue- And Muscle Fiber Type-Dependent Responses To Hypoxia And Hypercapnic Hypoxia In The Atlantic Blue Crab, Callinectes Sapidus, Kristin M. Hardy, Chandler R. Follett, Louis E. Burnett, Sean Lema

Biological Sciences

Hypoxia inducible factor (HIF) is a transcription factor that under low environmental oxygen regulates the expression of suites of genes involved in metabolism, angiogenesis, erythropoiesis, immune function, and growth. Here, we isolated and sequenced partial cDNAs encoding hif-α and arnt/hif-β from the Atlantic blue crab, Callinectes sapidus, an estuarine species that frequently encounters concurrent hypoxia (low O2) and hypercapnia (elevated CO2). We then examined the effects of acute exposure (1 h) to hypoxia (H) and hypercapnic hypoxia (HH) on relative transcript abundance for hif-α and arnt/hif-β in …


Investigation Of The Importance Of Rock Chemistry For Saxicolous Lichen Communities Of The New Idria Serpentinite Mass, San Benito County, California, Usa, Nishanta Rajakaruna, Kerry Knudsen, Alan M. Fryday, Ryan E. O'Dell, Nathaniel S. Pope, Fred C. Olday, Suzie Woolhouse Sep 2012

Investigation Of The Importance Of Rock Chemistry For Saxicolous Lichen Communities Of The New Idria Serpentinite Mass, San Benito County, California, Usa, Nishanta Rajakaruna, Kerry Knudsen, Alan M. Fryday, Ryan E. O'Dell, Nathaniel S. Pope, Fred C. Olday, Suzie Woolhouse

Biological Sciences

Although several lichen inventories exist for European ultramafic sites, only four surveys of serpentine lichens for North America have been published to date. Of those, only one has been conducted in California. We conducted a survey of saxicolous lichens from ultramafic rocks (including nephrite, partially serpentinized peridotite, and serpentinite) and non-ultramafic rocks (including silica-carbonate, shale, and sandstone) at the New Idria serpentinite mass, San Benito County, California. X-ray Fluorescence Analysis of the rocks from which the lichens were collected revealed significant elemental differences between the ultramafic and non-ultramafic rocks for 26 of the 32 major and trace elements analyzed. We …


Evaluation Of Headway G For Control Of Brown Patch Of Lawn Height Tall Fescue In Kansas, 2011, C Thompson, M Kennelly Aug 2012

Evaluation Of Headway G For Control Of Brown Patch Of Lawn Height Tall Fescue In Kansas, 2011, C Thompson, M Kennelly

Horticulture and Crop Science

No abstract provided.


Physiologically-Based Pharmacokinetic Modeling Of Acetaminophen Metabolism And Toxicity, David M. Ng, Ali Navid Aug 2012

Physiologically-Based Pharmacokinetic Modeling Of Acetaminophen Metabolism And Toxicity, David M. Ng, Ali Navid

STAR Program Research Presentations

Acetaminophen is a common analgesic and antipyretic. Metabolism of acetaminophen and acetaminophen-induced liver necrosis are predicted using physiologically-based pharmacokinetic (PBPK) modeling. Pharmacokinetic means the model determines where the drug is distributed in the body over time. Physiologically-based means the anatomy and physiology of the human body is reflected in the structure and functioning of the model. Acetaminophen is usually safe and effective when taken as recommended, but consumption at higher levels may lead to liver damage. Additionally, other factors such as alcoholic liver disease, smoking, and malnutrition affect the maximum safe dose of acetaminophen.


Improving The Efficiency Of Homologous Gene Replacement By Disrupting Non-Homologous End Joining Pathway Gene Kusa In The Oleaginous Fungus Mortierella Alpina, Kathleen T. Krueger, Ziyu Dai Dr, Ugur Uzuner, Scott E. Baker Dr Aug 2012

Improving The Efficiency Of Homologous Gene Replacement By Disrupting Non-Homologous End Joining Pathway Gene Kusa In The Oleaginous Fungus Mortierella Alpina, Kathleen T. Krueger, Ziyu Dai Dr, Ugur Uzuner, Scott E. Baker Dr

STAR Program Research Presentations

Mortierella alpina , a oleaginous filamentous fungus, is one of industrial fungal strains known for the production of arachidonic acid. It is also of particular interest for hydrocarbon biofuel production since it is able to produce up to 50% of its mass in rich, long-chain polyunsaturated fatty acids [PUFA’s]. In addition to high fatty acid production, M. alpina like many other oleaginous fungi, already have mechanisms for accumulating significant concentrations of hydrophobic compounds making it a naturally equipped candidate to handle potential toxic concentrations of hydrocarbons. To date, three different transformation methods have been established for M. alpina that are …


Nutritional Content Of Rhinoceros Auklet (Cerorhinca Monocerata) Bill Loads, Dustin Taylor, Ron Heintz Aug 2012

Nutritional Content Of Rhinoceros Auklet (Cerorhinca Monocerata) Bill Loads, Dustin Taylor, Ron Heintz

STAR Program Research Presentations

Nutritional Content of Rhinoceros Auklet Bill Loads

Dustin E Taylor

Abstract

An adult Rhinoceros Auklet (Cerorhinca monocerata) can only catch and carry a limited amount of prey to their nestlings after a foraging trip. The auklets therefore must maximize their efficiency by bringing back the most proportionally nutritious prey items to their nestlings. The prey carried back to the nesting sites (known as a ‘bill load’) can contain whole fish, as well as parts, most commonly fish heads. This study is aimed to determine whether returning with just heads to the nestlings was proportionally more nutritious than bringing …


A Genetic Survey Of English Sole Populations In The Salish Sea, Elizabeth S. Gutierrez, Gary A. Winans, Jon Baker, Amanda Cope Aug 2012

A Genetic Survey Of English Sole Populations In The Salish Sea, Elizabeth S. Gutierrez, Gary A. Winans, Jon Baker, Amanda Cope

STAR Program Research Presentations

This summer I interned at the Northwest Fisheries Science Center in Seattle, WA and participated in NOAA’s Salish Sea Project. The Salish Sea Project’s goal is to identify genetically distinctive groups of species in the Salish Sea that may have unique evolutionary and/or adaptive backgrounds. These findings will allow NOAA to promote and monitor the natural production of species in the Salish Sea, to select representative populations for experimental work regarding pollution, ocean acidification and climate change, to contribute to managing the ecosystem for intra- and inter-species diversity, and to help make informed decisions about adaptive management and marine protected …


Using Stable Isotope Analysis To Study Zooplankton Trophic Ecology In San Francisco Estuary, Steven C. Westbrook, Julien Moderan, Wim Kimmerer Aug 2012

Using Stable Isotope Analysis To Study Zooplankton Trophic Ecology In San Francisco Estuary, Steven C. Westbrook, Julien Moderan, Wim Kimmerer

STAR Program Research Presentations

Zooplankton biomasses in estuaries are often high and represent an important food source for fish, like theendangered Delta Smelt. Human interferences (nitrates from crops, freshwater flow alteration, invasive species introduction…) have altered the structure of the San Francisco Estuary (SFE) ecosystem. We use stable isotope analysis to improve our knowledge of the planktonic food web in the SFE and gain insights into its evolution over the past decades. Every living thing has a specific isotopic signature. For example, in the plankton we study exists Carbon 13 and Carbon 12. Carbon 13 is different only because it has one extra neutron, …


Functional Response Of Protected Larval Stage Delta Smelt (Hypomesus Transpacificus), Jorge Ruiz, Lindsay Sullivan, Wim Kimmerer, Joan Lindberg Aug 2012

Functional Response Of Protected Larval Stage Delta Smelt (Hypomesus Transpacificus), Jorge Ruiz, Lindsay Sullivan, Wim Kimmerer, Joan Lindberg

STAR Program Research Presentations

Delta smelt (Hypomesus transpacificus) is a protected slender-bodied fish endemic to the San Francisco Estuary. Smelt prey upon various zooplankton including the copepods such as Pseudodiaptomus forbesi. This experiment studied the prey maximum feeding rate of 2 larval stages (21 DHP, 35 DPH) of Delta smelt on various concentrations of P. forbesi. The copepod prey was offered at 7 different concentrations (2No./L–120No./L). After feeding was terminated the free prey and ingested prey were counted and analyzed to find the feeding rate of Delta smelt. Maximum feeding rate was found to be at much higher prey densities …


Quantification And In Vitro Analysis Of Nanolipoproteins (Nlps) Containing Adjuvants, Purna Venkataraman, Craig Blanchette, Nicholas O. Fischer Aug 2012

Quantification And In Vitro Analysis Of Nanolipoproteins (Nlps) Containing Adjuvants, Purna Venkataraman, Craig Blanchette, Nicholas O. Fischer

STAR Program Research Presentations

Nanolipoprotein particles (NLPs) self-assemble into nanoscale structures that can be used as vaccines or drug delivery agents. Due to the nature of the NLPs, a variety of immune stimulating compounds or adjuvants can be readily incorporated into NLPs: a characteristic difficult to engineer into most other nanoscale platforms. In light of this, a method for quantifying the amount adjuvant actually incorporated into NLPs is a question of high importance. Through the use of reverse phase High-Performance Liquid Chromatography (HPLC) and an Evaporative Light Scattering Detector (ELSD), standard curves can be constructed by analyzing mixtures of NLP components of known concentration, …